Publications
2016
Jasim Dhifaf A, Boutin Herve, Fairclough Michael, Ménard-Moyon Cécilia, Prenant Christian, Bianco Alberto, Kostarelos Kostas
Thickness of functionalized graphene oxide sheets plays critical role in tissue accumulation and urinary excretion: A pilot PET/CT study Journal Article
In: Applied Materials Today, vol. 4, pp. 24–30, 2016, ISSN: 2352-9407.
Abstract | Links | BibTeX | Tags: carbon, I2CT, imaging, Nanomedicine, Pharmacokinetics, Pharmacology, Team-Bianco
@article{jasim_thickness_2016,
title = {Thickness of functionalized graphene oxide sheets plays critical role in tissue accumulation and urinary excretion: A pilot PET/CT study},
author = {Dhifaf A Jasim and Herve Boutin and Michael Fairclough and Cécilia Ménard-Moyon and Christian Prenant and Alberto Bianco and Kostas Kostarelos},
url = {http://www.sciencedirect.com/science/article/pii/S2352940716300099},
doi = {10.1016/j.apmt.2016.04.003},
issn = {2352-9407},
year = {2016},
date = {2016-09-01},
urldate = {2020-04-01},
journal = {Applied Materials Today},
volume = {4},
pages = {24--30},
abstract = {We have recently reported that administration of thin graphene oxide (GO) sheets in the systemic circulation of rodents leads to rapid urinary excretion for the majority of injected dose and accumulation by the reticuloendothelial system organs for the remaining dose. In this study, graphene oxide was functionalized with a chelating moiety (DOTA, (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid)) and labeled with [64Cu] for positron emission computed tomography (PET/CT) imaging. The thin functionalized graphene oxide material (f-GO-thin) consisted of a few layers (∼5nm) in thickness. Aging of the f-GO-thin material led to re-stacking of the flakes that resulted in materials of increased thickness (f-GO-thick) without altering their lateral dimension. These two types of f-GOs were comparatively studied pharmacologically to reveal the previously unexplored in vivo role of graphene oxide sheet thickness. Our results showed that a significantly larger fraction of the thicker GO sheets (47.5% of injected dose) remained within the body of living animals 24h after intravenous administration, residing mainly in the spleen and liver. The thinner GO sheets were predominantly (76.9% of injected dose) excreted through the glomerular filter into the urine. This pilot study provides an initial correlation between graphene-based material structure and pharmacological profile that is imperative towards understanding of how 2D structures behave in vivo to give information on potential biomedical applications.},
keywords = {carbon, I2CT, imaging, Nanomedicine, Pharmacokinetics, Pharmacology, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
2014
Servant A, Bianco A, Prato M, Kostarelos K
Graphene for multi-functional synthetic biology: the last 'zeitgeist' in nanomedicine Journal Article
In: Bioorganic & Medicinal Chemistry Letters, vol. 24, no. 7, pp. 1638–1649, 2014, ISSN: 1464-3405.
Abstract | Links | BibTeX | Tags: Antineoplastic Agents, carbon, Drug delivery, Drug Design, Graphite, I2CT, Nanomaterials, Nanomedicine, nanotechnology, Synthetic Biology, Team-Bianco
@article{servant_graphene_2014,
title = {Graphene for multi-functional synthetic biology: the last 'zeitgeist' in nanomedicine},
author = {A Servant and A Bianco and M Prato and K Kostarelos},
doi = {10.1016/j.bmcl.2014.01.051},
issn = {1464-3405},
year = {2014},
date = {2014-01-01},
journal = {Bioorganic & Medicinal Chemistry Letters},
volume = {24},
number = {7},
pages = {1638--1649},
abstract = {The high versatility of graphene has attracted significant attention in many areas of scientific research from electronics to physics and mechanics. One of the most intriguing utilisation of graphene remains however in nanomedicine and synthetic biology. In particular, the last decade has witnessed an exponential growth in the generation of novel candidate therapeutics of multiple biological activities based on graphene constructs with small molecules, such as anti-cancer drugs. In this Digest, we summarise the different synthetic strategies and routes available to fabricate these promising graphene conjugates and the opportunities for the design of multi-functional tools for synthetic biology that they offer.},
keywords = {Antineoplastic Agents, carbon, Drug delivery, Drug Design, Graphite, I2CT, Nanomaterials, Nanomedicine, nanotechnology, Synthetic Biology, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Marangon Iris, Ménard‐Moyon Cécilia, Kolosnjaj‐Tabi Jelena, Béoutis Marie Lys, Lartigue Lénaic, Alloyeau Damien, Pach Elzbieta, Ballesteros Belén, Autret Gwennhael, Ninjbadgar Tsedev, Brougham Dermot F, Bianco Alberto, Gazeau Florence
Covalent Functionalization of Multi-walled Carbon Nanotubes with a Gadolinium Chelate for Efficient T1-Weighted Magnetic Resonance Imaging Journal Article
In: Advanced Functional Materials, vol. 24, no. 45, pp. 7173–7186, 2014, ISSN: 1616-3028.
Abstract | Links | BibTeX | Tags: Carbon nanotubes, contrast agents, I2CT, magnetic resonance imaging, Nanomedicine, Team-Bianco
@article{marangon_covalent_2014,
title = {Covalent Functionalization of Multi-walled Carbon Nanotubes with a Gadolinium Chelate for Efficient T1-Weighted Magnetic Resonance Imaging},
author = {Iris Marangon and Cécilia Ménard‐Moyon and Jelena Kolosnjaj‐Tabi and Marie Lys Béoutis and Lénaic Lartigue and Damien Alloyeau and Elzbieta Pach and Belén Ballesteros and Gwennhael Autret and Tsedev Ninjbadgar and Dermot F Brougham and Alberto Bianco and Florence Gazeau},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.201402234},
doi = {10.1002/adfm.201402234},
issn = {1616-3028},
year = {2014},
date = {2014-01-01},
urldate = {2020-04-02},
journal = {Advanced Functional Materials},
volume = {24},
number = {45},
pages = {7173--7186},
abstract = {Given the promise of carbon nanotubes (CNTs) for photothermal therapy, drug delivery, tissue engineering, and gene therapy, there is a need for non-invasive imaging methods to monitor CNT distribution and fate in the body. In this study, non-ionizing whole-body high field magnetic resonance imaging (MRI) is used to follow the distribution of water-dispersible non-toxic functionalized CNTs administrated intravenously to mice. Oxidized CNTs are endowed with positive MRI contrast properties by covalent functionalization with the chelating ligand diethylenetriaminepentaacetic dianhydride (DTPA), followed by chelation to Gd3+. The structural and magnetic properties, MR relaxivities, cellular uptake, and application for MRI cell imaging of Gd-CNTs in comparison to the precursor oxidized CNTs are evaluated. Despite the intrinsic T2 contrast of oxidized CNTs internalized in macrophages, the anchoring of paramagnetic gadolinium onto the nanotube sidewall allows efficient T1 contrast and MR signal enhancement, which is preserved after CNT internalization by cells. Hence, due to their high dispersibility, Gd-CNTs have the potential to produce positive contrast in vivo following injection into the bloodstream. The uptake of Gd-CNTs in the liver and spleen is assessed using MRI, while rapid renal clearance of extracellular Gd-CNTs is observed, confirming the evidences of other studies using different imaging modalities.},
keywords = {Carbon nanotubes, contrast agents, I2CT, magnetic resonance imaging, Nanomedicine, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
2011
Al-Jamal Khuloud T, Gherardini Lisa, Bardi Giuseppe, Nunes Antonio, Guo Chang, Bussy Cyrill, Herrero Antonia M, Bianco Alberto, Prato Maurizio, Kostarelos Kostas, Pizzorusso Tommaso
Functional motor recovery from brain ischemic insult by carbon nanotube-mediated siRNA silencing Journal Article
In: Proceedings of the National Academy of Sciences of the United States of America, vol. 108, no. 27, pp. 10952–10957, 2011, ISSN: 1091-6490.
Abstract | Links | BibTeX | Tags: Animals, Apoptosis, Base Sequence, Brain Ischemia, carbon, Caspase 3, Caspase Inhibitors, Cell Line, Cells, Cultured, Electron, Endothelin-1, Female, Genetic Therapy, I2CT, Inbred C57BL, Mice, Microscopy, Nanomedicine, Nanotubes, Neurons, Psychomotor Performance, Rats, RNA, RNA Interference, Small Interfering, Sprague-Dawley, Team-Bianco, Transmission
@article{al-jamal_functional_2011,
title = {Functional motor recovery from brain ischemic insult by carbon nanotube-mediated siRNA silencing},
author = {Khuloud T Al-Jamal and Lisa Gherardini and Giuseppe Bardi and Antonio Nunes and Chang Guo and Cyrill Bussy and Antonia M Herrero and Alberto Bianco and Maurizio Prato and Kostas Kostarelos and Tommaso Pizzorusso},
doi = {10.1073/pnas.1100930108},
issn = {1091-6490},
year = {2011},
date = {2011-07-01},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {108},
number = {27},
pages = {10952--10957},
abstract = {Stroke is the second cause of death worldwide with ischemic stroke accounting for 80% of all stroke insults. Caspase-3 activation contributes to brain tissue loss and downstream biochemical events that lead to programmed cell death after traumatic brain injury. Alleviation of symptoms following ischemic neuronal injury can be potentially achieved by either genetic disruption or pharmacological inhibition of caspases. Here, we studied whether silencing of Caspase-3 using carbon nanotube-mediated in vivo RNA interference (RNAi) could offer a therapeutic opportunity against stroke. Effective delivery of siRNA directly to the CNS has been shown to normalize phenotypes in animal models of several neurological diseases. It is shown here that peri-lesional stereotactic administration of a Caspase-3 siRNA (siCas 3) delivered by functionalized carbon nanotubes (f-CNT) reduced neurodegeneration and promoted functional preservation before and after focal ischemic damage of the rodent motor cortex using an endothelin-1 induced stroke model. These observations illustrate the opportunity offered by carbon nanotube-mediated siRNA delivery and gene silencing of neuronal tissue applicable to a variety of different neuropathological conditions where intervention at well localized brain foci may offer therapeutic and functional benefits.},
keywords = {Animals, Apoptosis, Base Sequence, Brain Ischemia, carbon, Caspase 3, Caspase Inhibitors, Cell Line, Cells, Cultured, Electron, Endothelin-1, Female, Genetic Therapy, I2CT, Inbred C57BL, Mice, Microscopy, Nanomedicine, Nanotubes, Neurons, Psychomotor Performance, Rats, RNA, RNA Interference, Small Interfering, Sprague-Dawley, Team-Bianco, Transmission},
pubstate = {published},
tppubtype = {article}
}
2009
Kostarelos K, Bianco A, Prato M
Promises, facts and challenges for carbon nanotubes in imaging and therapeutics Journal Article
In: Nature Nanotechnology, vol. 4, no. 10, pp. 627–633, 2009, ISSN: 1748-3395.
Abstract | Links | BibTeX | Tags: Animals, carbon, Diagnostic Imaging, Drug Evaluation, Humans, I2CT, Nanomedicine, Nanotubes, Preclinical, Team-Bianco, therapeutics
@article{kostarelos_promises_2009,
title = {Promises, facts and challenges for carbon nanotubes in imaging and therapeutics},
author = {K Kostarelos and A Bianco and M Prato},
doi = {10.1038/nnano.2009.241},
issn = {1748-3395},
year = {2009},
date = {2009-10-01},
journal = {Nature Nanotechnology},
volume = {4},
number = {10},
pages = {627--633},
abstract = {The use of carbon nanotubes in medicine is now at the crossroads between a proof-of-principle concept and an established preclinical candidate for a variety of therapeutic and diagnostic applications. Progress towards clinical trials will depend on the outcomes of efficacy and toxicology studies, which will provide the necessary risk-to-benefit assessments for carbon-nanotube-based materials. Here we focus on carbon nanotubes that have been studied in preclinical animal models, and draw attention to the promises, facts and challenges of these materials as they transition from research to the clinical phase. We address common questions regarding the use of carbon nanotubes in disease imaging and therapy, and highlight the opportunities and challenges ahead.},
keywords = {Animals, carbon, Diagnostic Imaging, Drug Evaluation, Humans, I2CT, Nanomedicine, Nanotubes, Preclinical, Team-Bianco, therapeutics},
pubstate = {published},
tppubtype = {article}
}
Podesta Jennifer E, Al-Jamal Khuloud T, Herrero Antonia M, Tian Bowen, Ali-Boucetta Hanene, Hegde Vikas, Bianco Alberto, Prato Maurizio, Kostarelos Kostas
Antitumor activity and prolonged survival by carbon-nanotube-mediated therapeutic siRNA silencing in a human lung xenograft model Journal Article
In: Small (Weinheim an Der Bergstrasse, Germany), vol. 5, no. 10, pp. 1176–1185, 2009, ISSN: 1613-6829.
Abstract | Links | BibTeX | Tags: Animals, Antineoplastic Agents, Apoptosis, carbon, Cell Line, Cell Proliferation, Electrophoresis, Gene Silencing, Humans, I2CT, Liposomes, Lung Neoplasms, Mice, Nanomedicine, Nanotubes, RNA, Small Interfering, Survival Analysis, Team-Bianco, tumor, Xenograft Model Antitumor Assays
@article{podesta_antitumor_2009,
title = {Antitumor activity and prolonged survival by carbon-nanotube-mediated therapeutic siRNA silencing in a human lung xenograft model},
author = {Jennifer E Podesta and Khuloud T Al-Jamal and Antonia M Herrero and Bowen Tian and Hanene Ali-Boucetta and Vikas Hegde and Alberto Bianco and Maurizio Prato and Kostas Kostarelos},
doi = {10.1002/smll.200801572},
issn = {1613-6829},
year = {2009},
date = {2009-05-01},
journal = {Small (Weinheim an Der Bergstrasse, Germany)},
volume = {5},
number = {10},
pages = {1176--1185},
abstract = {Carbon nanotubes are novel nanomaterials that are thought to offer potential benefits to a variety of biomedical and clinical applications. In this study, the treatment of a human lung carcinoma model in vivo using siRNA sequences leading to cytotoxicity and cell death is carried out using either cationic liposomes (DOTAP:cholesterol) or amino-functionalized multi-walled carbon nanotubes (MWNT - NH(+)(3)). Validation for the most cytotoxic siRNA sequence using a panel of human carcinoma and murine cells reveals that the proprietary siTOX sequence is human specific and can lead to significant cytotoxic activities delivered both by liposome or MWNT - NH(+)(3) in vitro. A comparative study using both types of vector indicates that only MWNT - NH(+)(3):siRNA complexes administered intratumorally can elicit delayed tumor growth and increased survival of xenograft-bearing animals. siTOX delivery via the cationic MWNT - NH(+)(3) is biologically active in vivo by triggering an apoptotic cascade, leading to extensive necrosis of the human tumor mass. This suggests that carbon-nanotube-mediated delivery of siRNA by intratumoral administration leads to successful and statistically significant suppression of tumor volume, followed by a concomitant prolongation of survival of human lung tumor-bearing animals. The direct comparison between carbon nanotubes and liposomes demonstrates the potential advantages offered by carbon nanotubes for the intracellular delivery of therapeutic agents in vivo. The present work may act as the impetus for further studies to explore the therapeutic capacity of chemically functionalized carbon nanotubes to deliver siRNA directly into the cytoplasm of target cells and achieve effective therapeutic silencing in various disease indications where local delivery is feasible or desirable.},
keywords = {Animals, Antineoplastic Agents, Apoptosis, carbon, Cell Line, Cell Proliferation, Electrophoresis, Gene Silencing, Humans, I2CT, Liposomes, Lung Neoplasms, Mice, Nanomedicine, Nanotubes, RNA, Small Interfering, Survival Analysis, Team-Bianco, tumor, Xenograft Model Antitumor Assays},
pubstate = {published},
tppubtype = {article}
}
2008
Kostarelos Kostas, Bianco Alberto, Prato Maurizio
Hype around nanotubes creates unrealistic hopes Journal Article
In: Nature, vol. 453, no. 7193, pp. 280, 2008, ISSN: 1476-4687.
Links | BibTeX | Tags: Adult Stem Cells, carbon, Humans, I2CT, Nanomedicine, Nanotubes, Reproducibility of Results, Team-Bianco
@article{kostarelos_hype_2008,
title = {Hype around nanotubes creates unrealistic hopes},
author = {Kostas Kostarelos and Alberto Bianco and Maurizio Prato},
doi = {10.1038/453280c},
issn = {1476-4687},
year = {2008},
date = {2008-05-01},
journal = {Nature},
volume = {453},
number = {7193},
pages = {280},
keywords = {Adult Stem Cells, carbon, Humans, I2CT, Nanomedicine, Nanotubes, Reproducibility of Results, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Bianco Alberto, Kostarelos Kostas, Prato Maurizio
Opportunities and challenges of carbon-based nanomaterials for cancer therapy Journal Article
In: Expert Opinion on Drug Delivery, vol. 5, no. 3, pp. 331–342, 2008, ISSN: 1742-5247.
Abstract | Links | BibTeX | Tags: Animals, carbon, Electron, Humans, I2CT, Microscopy, Nanomedicine, Nanostructures, Nanotubes, Neoplasms, Pharmaceutical, Team-Bianco, Technology, Transmission
@article{bianco_opportunities_2008,
title = {Opportunities and challenges of carbon-based nanomaterials for cancer therapy},
author = {Alberto Bianco and Kostas Kostarelos and Maurizio Prato},
doi = {10.1517/17425247.5.3.331},
issn = {1742-5247},
year = {2008},
date = {2008-01-01},
journal = {Expert Opinion on Drug Delivery},
volume = {5},
number = {3},
pages = {331--342},
abstract = {The possibility of incorporating carbon-based nanomaterials into living systems has opened the way for the investigation of their potential applications in the emerging field of nanomedicine. A wide variety of different nanomaterials based on allotropic forms of carbon, such as nanotubes, nanohorns and nanodiamonds, are currently being explored towards different biomedical applications. In this review, we discuss the recent advances in the development of these novel nanomaterials for cancer therapy. A comparison between the characteristics, the advantages, the drawbacks, the benefits and the risks associated with these novel biocompatible forms of carbon is presented here.},
keywords = {Animals, carbon, Electron, Humans, I2CT, Microscopy, Nanomedicine, Nanostructures, Nanotubes, Neoplasms, Pharmaceutical, Team-Bianco, Technology, Transmission},
pubstate = {published},
tppubtype = {article}
}
2006
Lacerda Lara, Bianco Alberto, Prato Maurizio, Kostarelos Kostas
Carbon nanotubes as nanomedicines: from toxicology to pharmacology Journal Article
In: Advanced Drug Delivery Reviews, vol. 58, no. 14, pp. 1460–1470, 2006, ISSN: 0169-409X.
Abstract | Links | BibTeX | Tags: Animals, carbon, Humans, I2CT, Nanomedicine, Nanotubes, Pharmacokinetics, Pharmacology, Team-Bianco
@article{lacerda_carbon_2006,
title = {Carbon nanotubes as nanomedicines: from toxicology to pharmacology},
author = {Lara Lacerda and Alberto Bianco and Maurizio Prato and Kostas Kostarelos},
doi = {10.1016/j.addr.2006.09.015},
issn = {0169-409X},
year = {2006},
date = {2006-12-01},
journal = {Advanced Drug Delivery Reviews},
volume = {58},
number = {14},
pages = {1460--1470},
abstract = {Various biomedical applications of carbon nanotubes have been proposed in the last few years leading to the emergence of a new field in diagnostics and therapeutics. Most of these applications will involve the administration or implantation of carbon nanotubes and their matrices into patients. The toxicological and pharmacological profile of such carbon nanotube systems developed as nanomedicines will have to be determined prior to any clinical studies undertaken. This review brings together all the toxicological and pharmacological in vivo studies that have been carried out using carbon nanotubes, to offer the first summary of the state-of-the-art in the pharmaceutical development of carbon nanotubes on the road to becoming viable and effective nanomedicines.},
keywords = {Animals, carbon, Humans, I2CT, Nanomedicine, Nanotubes, Pharmacokinetics, Pharmacology, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
2005
Kostarelos K, Lacerda L, Partidos C D, Prato M, Bianco A
Carbon nanotube-mediated delivery of peptides and genes to cells: translating nanobiotechnology to therapeutics Journal Article
In: Journal of Drug Delivery Science and Technology, vol. 15, no. 1, pp. 41–47, 2005, ISSN: 1773-2247.
Abstract | Links | BibTeX | Tags: Carbon nanotubes, gene delivery, gene therapy, I2CT, Nanomedicine, Peptide delivery, Team-Bianco, Vaccination
@article{kostarelos_carbon_2005,
title = {Carbon nanotube-mediated delivery of peptides and genes to cells: translating nanobiotechnology to therapeutics},
author = {K Kostarelos and L Lacerda and C D Partidos and M Prato and A Bianco},
url = {http://www.sciencedirect.com/science/article/pii/S1773224705500054},
doi = {10.1016/S1773-2247(05)50005-4},
issn = {1773-2247},
year = {2005},
date = {2005-01-01},
urldate = {2020-03-31},
journal = {Journal of Drug Delivery Science and Technology},
volume = {15},
number = {1},
pages = {41--47},
abstract = {During the last few years, there has been a tremendous amount of optimism and expectation about nanotechnology and its impact on various fields including medicine and pharmaceutical development. One of the most promising materials being developed during the nanotechnological renaissance we are currently experiencing is the carbon nanotube. Before any biology-related application can even be envisaged, the aqueous solubility of carbon nanotubes has to be resolved. Recently, a variety of methodologies have been proposed which lead to biologically compatible carbon nanotubes. Covalent functionalization of their surface is one methodology, allowing the first attempts towards applications in the field of nanomedicine. The possibility of incorporating functionalized carbon nanotubes into cells and the biological milieu offers numerous advantages for potential applications in biology and pharmacology. One of the most promising is their utilization as a new carrier system for the delivery of therapeutic molecules. In the present article, the first attempts to transform carbon nanotubes from biologically incompatible nanomaterials to biologically relevant components of advanced therapeutics and the ensuing novel structures obtained in our laboratories are presented.},
keywords = {Carbon nanotubes, gene delivery, gene therapy, I2CT, Nanomedicine, Peptide delivery, Team-Bianco, Vaccination},
pubstate = {published},
tppubtype = {article}
}