ARN

Fixed-term contract,Researcher

The project will consist in studying the innate immune response during infection of human cells, mosquitoes and ticks by the Zika virus (ZIKV) and tick-borne encephalitis virus (TBEV). More specifically, the aim will be to characterize the interactome of the viral RNA replication intermediate in order to identify new factors in antiviral response. Mass spectrometry and high throughput sequencing approaches will be implemented in these infection models.

Fixed-term contract,Researcher

The mission of the project will consist in the analysis of RNA modifications (mainly tRNA and rRNA) in different strains of Staphylococcus aureus and in different stress conditions encountered during infections. This mapping will be performed using innovative methodologies, including mass spectrometry and high-throughput sequencing. The impact of variations in the epitranscriptome on the translation process and on the decoding rules will be analyzed at global scale by means of "ribosome profiling" and in details by in vitro translation systems. Based on the obtained results, mutations in key modifying enzymes will be analyzed for their function in the physiology and pathogenicity of S. aureus.

Fixed-term contract,Researcher

The project will analyse the impact of myeloperoxidase-derived oxidants during interactions between neutrophils and the pathogenic bacterium Salmonella. In particular, the project will develop cellular models (i.e. immune cells) for the study of host-pathogen interactions.

Fixed-term contract,Researcher

The droplet microfluidic systems used by the "Digital RNA Biology" team (Institute of Molecular and Cellular Biology, Strasbourg) allow the production and manipulation (e.g., controlled melting, sorting) of water-in-oil microdroplets of a few picolitres in size and very homogeneous in size at a rate of several thousand events per second. The present project will aim to use biomolecule capture technology to coat the inner surface of the droplets with synthetic RNA motifs. This approach will also allow the identification of molecules capable of interfering with the RNA/protein interaction.

Fixed-term contract,Researcher

The mission of the project will consist in analyzing the function of regulatory RNAs of Staphylococcus aureus in an infectious context. In particular, the project will require the development and use of eukaryotic cells (i.e. immune cells) and mouse models for the study of host-pathogenic bacteria interactions. Different high-throughput sequencing approaches will be used to analyze RNA expression in these different infection models.