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Missions :
- The Animal Experimentation technical platform is in charge of the implementation of experimental procedures as part of protocols established by researchers, as well as the breeding management of murine strains.
Activities :
- The technical platform manage the breeding of around thirty different mouse strains in connection with researchers, including some intricate transgenic strain. Several murine models are used, notably basic inbred strains as well as lupic mice models.
- The Animal Experimentation platform team carries out the protocols requested by the different teams of the Unit in compliance with ethics and regulations in force.
- The platform agents also perform different molecular biology techniques to genotype the Unit’s transgenic strains.
Composition :
- 2 dedicated technicians
Link :
Missions :
We produce, by solid phase synthesis and Fmoc strategy, a wide variety of peptides for our customers (public research or industry)
Activities:
Under standard conditions, the length of the peptides ranges from 2 to 60 AA, the synthesis scale from 25 to 100 micromoles, and the purity is greater than 95%
Many modifications can be introduced: N-terminal acetylation, C-terminal amidation, biotinylation, conjugation (to KLH, BSA…), incorporation of exotic amino acid or fluorescent tags…
The peptides are purified and analyzed by RP-HPLC, lyophilized and then characterized by mass spectrometry (LC/MS)
The peptides are supplied with an analysis report (HPLC and mass spectrum)
Information request:
For all information requests ( peptide modifications, quotation, deadline…): Olivier Chaloin
Presentation of the UPR3572 imaging technical platform
The imaging technical platform belongs to the UPR3572 research unit of the IBMC. Its objective is to make microscopy tools and techniques available to this unit to answer the biological questions raised during the study of immune responses in normal or pathological contexts. The technical platform also works in collaboration with the UPR9002 and UPR9022 research units of the institute and interacts closely with other Strasbourg imaging platforms.
Missions
- Develop or implement and implement imaging tools and techniques.
- Train, help and advise users
- Maintening the optimal performance of the systems
- Ensure a technological watch.
- Offer support for researchers’ projects.
Equipment
- Manufacturer: Leica
- Model: Ultracut R
- Manufacturer: Leica
- Model: RM2235
- Manufacturer: Leica
- Model: RM2235
- Manufacturer: Zeiss
- Model: Axiovert 40CFL
- Microscope: Inverted phase contrast
- Objectives:
| Magnifiaction | Type | NA | Coverslip correction | Immersion | Contraste | Reference |
| 5x | CP Achromat | 0,12 | Dry | 440920 | ||
| 10x | A-Plan | 0,25 | Dry | Ph1 | 441031 | |
| 20x | LD A-Plan | 0.3 | 1 | Dry | Ph1 | 1006591 |
- Camera : Axiocam ICc 1
- Software : Axiovision
- Manufacturer: Nikon
- Model: TS100
- Microscope: Inverted phase contrast
- Objectives:
| Magnifiaction | Type | NA | Coverslip correction | Immersion | Contraste | Reference |
| 4x | CFI Achro | 0,1 | Dry | MRP00042 | ||
| 10x | CFI Achro | 0,25 | 1.2 | Dry | Ph1 ADL | MRP40102 |
| 20x | CFI Achro LWD | 0.4 | 1.2 | Dry | Ph1 ADL | MRP46202 |
| 40x | CFI Achro LWD | 0.55 | 1.2 | Dry | Ph1 ADL | MRP46402 |
- Manufacturer: Motic
- Model: AE2000
- Microscope: Inverted phase contrast
- Objectives:
| Magnifiaction | Type | NA | Coverslip correction | Immersion | Contraste | |
| 4x | Plan | 0,1 | Dry | |||
| 10x | Plan | 0,25 | 1.1 | Dry | Ph1 | |
| 20x | LWD Plan | 0.3 | 1.1 | Dry | Ph1 | |
| 40x | LWD Plan | 0.5 | 1.1 | Dry | Ph1 |
- Manufacturer: Leica
- Model: DME
- Microscope: Upright phase contrast
- Objectives:
| Magnifiaction | Type | NA | Coverslip correction | Immersion | Contraste | Reference |
| 4x | HI Plan | 0,1 | Dry | 506266 | ||
| 10x | C Plan | 0,25 | Dry | Ph1 | 506078 | |
| 40x | C Plan | 0.3 | 0,17 | Dry | Ph2 | 506080 |
| 100x | C Plan | 0.5 | 0,17 | Oil | Ph3 | 506073 |
- Manufacturer: Zeiss
- Model: Axio Observer Z1
- Microscope: Inverted with differential interference contrast
- Advance device : Yokogawa CSU confocal head
- Motorized devices:
- Objective turret
- filter wheel
- Stage
- Shutter
- Objectives:
| Magnifiaction | Type | NA | Coverslip correction | Immersion | Contraste | Reference |
| 10x | EC Plan-Neofluar | 0.3 | 0.17 | Dry | Ph1 | 420341 9911 |
| 20x | Plan-Apochromat | 0.8 | 0.17 | Dry | DIC2 | 420650 9901 |
| 40x | Plan-Apochromat | 1.4 | 0.17 | Oil | DIC3 | 420762 9900 |
| 63x | Plan-Apochromat | 1.4 | 0.17 | Oil | DIC3 | 420782 9900 |
| 100x | Plan-Apochromat | 1.4 | 0.17 | Oil | DIC3 | 420792 9900 |
- Wide-field fluorescence illumination
- Source : Spectra X
- Filter cubes:
| Name | Excitation (nm) | Dichroic (nm) | Emission (nm) | |
| Set 49 | G 365 (300-400) | 395 | 420-470 | |
| Set 44 | 455-495 | 510 | 505-555 | |
| Set 43 | 532.5-557.5 | 570 | 570-640 | |
| Set 50 | 625-655 | 660 | 665-715 |
- Confocal fluorescence illumintation:
- Excitation : 405, 488, 561, 633
- Dichroic: QDM 405+488+561+640; 13x15x0.5 (E)
- Emission:
- BP450/50
- BP525/50
- BP629/62
- BP690/50
