Imaging

@article{,

title = {Cleavage of RNA with synthetic ribonuclease mimics},

author = {R Giege and B Felden and M A Zenkova and V N Sil'nikov and V V Vlassov},

url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10889986},

isbn = {10889986},

year  = {2000},

date = {2000-01-01},

journal = {Methods Enzymol},

volume = {318},

pages = {147-165},

note = {0076-6879 

Journal Article},

keywords = {Asp/chemistry  Ribonuclease, Base Sequence  Electrophoresis, Chemical  Molecular Sequence Data  Nucleic Acid Conformation  Nucleic Acid Hybridization  Phosphorylation  Plasmids/metabolism  RNA/chemistry/*metabolism  RNA, Non-U.S. Gov't, Pancreatic/chemistry/pharmacology  Ribonucleases/*chemistry/pharmacology  Saccharomyces cerevisiae/genetics  Spectrophotometry  Support, Polyacrylamide Gel  Genetic Techniques  Hydrolysis  Imidazoles/pharmacology  Models, Transfer, Unité ARN},

pubstate = {published},

tppubtype = {article}

}

@article{,

title = {Hybridization of antisense oligonucleotides with the 3'part of tRNA(Phe)},

author = {V A Petyuk and M A Zenkova and R Giege and V V Vlassov},

url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10050762},

isbn = {10050762},

year  = {1999},

date = {1999-01-01},

journal = {FEBS Lett},

volume = {444},

number = {2-3},

pages = {217-221},

abstract = {The interaction of antisense oligodeoxyribonucleotides with yeast tRNA(Phe) was investigated. 14-15-mers complementary to the 3'-terminal sequence including the ACCA end bind to the tRNA under physiological conditions. At low oligonucleotide concentrations the binding occurs at the unique complementary site. At higher oligonucleotide concentrations, the second oligonucleotide molecule binds to the complex due to non-perfect duplex formation in the T-loop stabilized by stacking between the two bound oligonucleotides. In these complexes the acceptor stem is open and the 5'-terminal sequence of the tRNA is accessible for binding of a complementary oligonucleotide. The results prove that the efficient binding of oligonucleotides to the 3'-terminal sequence of the tRNA occurs through initial binding to the single-stranded sequence ACCA followed by invasion in the acceptor stem and strand displacement.},

note = {0014-5793 

Journal Article},

keywords = {Antisense/*genetics  RNA, Base Sequence  Electrophoresis, Calf Thymus/metabolism  Support, Fungal/genetics  RNA, Non-U.S. Gov't, Phe/*genetics  Ribonuclease H, Polyacrylamide Gel  Molecular Sequence Data  Nucleic Acid Conformation  Nucleic Acid Hybridization/*genetics  Oligodeoxyribonucleotides/genetics  Oligonucleotides, Transfer, Unité ARN},

pubstate = {published},

tppubtype = {article}

}