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1999
Petyuk V A, Zenkova M A, Giege R, Vlassov V V
Hybridization of antisense oligonucleotides with the 3'part of tRNA(Phe) Journal Article
In: FEBS Lett, vol. 444, no. 2-3, pp. 217-221, 1999, ISBN: 10050762, (0014-5793 Journal Article).
Abstract | Links | BibTeX | Tags: Antisense/*genetics RNA, Base Sequence Electrophoresis, Calf Thymus/metabolism Support, Fungal/genetics RNA, Non-U.S. Gov't, Phe/*genetics Ribonuclease H, Polyacrylamide Gel Molecular Sequence Data Nucleic Acid Conformation Nucleic Acid Hybridization/*genetics Oligodeoxyribonucleotides/genetics Oligonucleotides, Transfer, Unité ARN
@article{,
title = {Hybridization of antisense oligonucleotides with the 3'part of tRNA(Phe)},
author = {V A Petyuk and M A Zenkova and R Giege and V V Vlassov},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10050762},
isbn = {10050762},
year = {1999},
date = {1999-01-01},
journal = {FEBS Lett},
volume = {444},
number = {2-3},
pages = {217-221},
abstract = {The interaction of antisense oligodeoxyribonucleotides with yeast tRNA(Phe) was investigated. 14-15-mers complementary to the 3'-terminal sequence including the ACCA end bind to the tRNA under physiological conditions. At low oligonucleotide concentrations the binding occurs at the unique complementary site. At higher oligonucleotide concentrations, the second oligonucleotide molecule binds to the complex due to non-perfect duplex formation in the T-loop stabilized by stacking between the two bound oligonucleotides. In these complexes the acceptor stem is open and the 5'-terminal sequence of the tRNA is accessible for binding of a complementary oligonucleotide. The results prove that the efficient binding of oligonucleotides to the 3'-terminal sequence of the tRNA occurs through initial binding to the single-stranded sequence ACCA followed by invasion in the acceptor stem and strand displacement.},
note = {0014-5793
Journal Article},
keywords = {Antisense/*genetics RNA, Base Sequence Electrophoresis, Calf Thymus/metabolism Support, Fungal/genetics RNA, Non-U.S. Gov't, Phe/*genetics Ribonuclease H, Polyacrylamide Gel Molecular Sequence Data Nucleic Acid Conformation Nucleic Acid Hybridization/*genetics Oligodeoxyribonucleotides/genetics Oligonucleotides, Transfer, Unité ARN},
pubstate = {published},
tppubtype = {article}
}
The interaction of antisense oligodeoxyribonucleotides with yeast tRNA(Phe) was investigated. 14-15-mers complementary to the 3'-terminal sequence including the ACCA end bind to the tRNA under physiological conditions. At low oligonucleotide concentrations the binding occurs at the unique complementary site. At higher oligonucleotide concentrations, the second oligonucleotide molecule binds to the complex due to non-perfect duplex formation in the T-loop stabilized by stacking between the two bound oligonucleotides. In these complexes the acceptor stem is open and the 5'-terminal sequence of the tRNA is accessible for binding of a complementary oligonucleotide. The results prove that the efficient binding of oligonucleotides to the 3'-terminal sequence of the tRNA occurs through initial binding to the single-stranded sequence ACCA followed by invasion in the acceptor stem and strand displacement.
1997
Wilhelm M, Heyman T, Friant S, Wilhelm F X
Heterogeneous terminal structure of Ty1 and Ty3 reverse transcripts Journal Article
In: Nucleic Acids Res, vol. 25, no. 11, pp. 2161-2166, 1997, ISBN: 9153316, (0305-1048 Journal Article).
Abstract | Links | BibTeX | Tags: Calf Thymus/metabolism Support, DNA Replication DNA, Fungal/*chemistry/metabolism *Nucleic Acid Conformation Nucleic Acid Hybridization Plasmids/chemistry/genetics/metabolism Polymerase Chain Reaction RNA, Genetic, Non-U.S. Gov't *Transcription, Transfer/chemistry Retroelements/*genetics Ribonuclease H, Unité ARN
@article{,
title = {Heterogeneous terminal structure of Ty1 and Ty3 reverse transcripts},
author = {M Wilhelm and T Heyman and S Friant and F X Wilhelm},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9153316},
isbn = {9153316},
year = {1997},
date = {1997-01-01},
journal = {Nucleic Acids Res},
volume = {25},
number = {11},
pages = {2161-2166},
abstract = {A specific terminal structure of preintegrative DNA is required for transposition of retroviruses and LTR-retrotransposons. We have used an anchored PCR technique to map the 3'ends of DNA intermediates synthesized inside yeast Ty1 and Ty3 retrotransposon virus-like particles. We find that, unlike retroviruses, Ty1 replicated DNA does not have two extra base pairs at its 3'ends. In contrast some Ty3 preintegrative DNA molecules have two extra nucleotides at the 3'end of upstream and downstream long terminal repeats. Moreover we find that some molecules of replicated Ty3 DNA have more than two extra nucleotides at the 3'end of the upstream LTR. This observation could be accounted for by imprecise RNAse H cutting of the PPT sequence. The site of Ty1 and Ty3 plus-strand strong-stop DNA termination was also examined. Our results confirm that the prominent Ty1 and Ty3 plus-strand strong-stop molecules harbor 12 tRNA templated bases but also show that some Ty1 and Ty3 plus-strand strong-stop DNA molecules harbor less tRNA templated bases. We propose that these less than full length plus-strand molecules could be active intermediates in Ty retrotransposon replication.},
note = {0305-1048
Journal Article},
keywords = {Calf Thymus/metabolism Support, DNA Replication DNA, Fungal/*chemistry/metabolism *Nucleic Acid Conformation Nucleic Acid Hybridization Plasmids/chemistry/genetics/metabolism Polymerase Chain Reaction RNA, Genetic, Non-U.S. Gov't *Transcription, Transfer/chemistry Retroelements/*genetics Ribonuclease H, Unité ARN},
pubstate = {published},
tppubtype = {article}
}
A specific terminal structure of preintegrative DNA is required for transposition of retroviruses and LTR-retrotransposons. We have used an anchored PCR technique to map the 3'ends of DNA intermediates synthesized inside yeast Ty1 and Ty3 retrotransposon virus-like particles. We find that, unlike retroviruses, Ty1 replicated DNA does not have two extra base pairs at its 3'ends. In contrast some Ty3 preintegrative DNA molecules have two extra nucleotides at the 3'end of upstream and downstream long terminal repeats. Moreover we find that some molecules of replicated Ty3 DNA have more than two extra nucleotides at the 3'end of the upstream LTR. This observation could be accounted for by imprecise RNAse H cutting of the PPT sequence. The site of Ty1 and Ty3 plus-strand strong-stop DNA termination was also examined. Our results confirm that the prominent Ty1 and Ty3 plus-strand strong-stop molecules harbor 12 tRNA templated bases but also show that some Ty1 and Ty3 plus-strand strong-stop DNA molecules harbor less tRNA templated bases. We propose that these less than full length plus-strand molecules could be active intermediates in Ty retrotransposon replication.
