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2023
PELLETIER Julien, DAWIT Mengistu, GHANINIA Majid, MAROIS Eric, IGNELL Rickard
A mosquito-specific antennal protein is critical for the attraction to human odor in the malaria vector Anopheles gambiae Article de journal
Dans: Insect Biochemistry and Molecular Biology, vol. 159, iss. August 2023, 2023.
Résumé | Liens | BibTeX | Étiquettes: antenna, chemoreceptor, M3i, marois, mosquitoes, olfaction
@article{IGNELL2023,
title = {A mosquito-specific antennal protein is critical for the attraction to human odor in the malaria vector Anopheles gambiae},
author = {Julien PELLETIER AND Mengistu DAWIT AND Majid GHANINIA AND Eric MAROIS AND Rickard IGNELL},
editor = { },
url = {https://doi.org/10.1016/j.ibmb.2023.103988},
doi = {j.ibmb.2023.103988},
year = {2023},
date = {2023-07-11},
urldate = {2023-07-11},
journal = {Insect Biochemistry and Molecular Biology},
volume = {159},
issue = {August 2023},
abstract = {Mosquitoes rely mainly on the sense of smell to decipher their environment and locate suitable food sources, hosts for blood feeding and oviposition sites. The molecular bases of olfaction involve multigenic families of olfactory proteins that have evolved to interact with a narrow set of odorants that are critical for survival. Understanding the complex interplay between diversified repertoires of olfactory proteins and ecologically-relevant odorant signals, which elicit important behaviors, is fundamental for the design of novel control strategies targeting the sense of smell of disease vector mosquitoes. Previously, large multigene families of odorant receptor and ionotropic receptor proteins, as well as a subset of odorant-binding proteins have been shown to mediate the selectivity and sensitivity of the mosquito olfactory system. In this study, we identify a mosquito-specific antennal protein (MSAP) gene as a novel molecular actor of odorant reception. MSAP is highly conserved across mosquito species and is transcribed at an extremely high level in female antennae. In order to understand its role in the mosquito olfactory system, we generated knockout mutant lines in Anopheles gambiae, and performed comparative analysis of behavioral and physiological responses to human-associated odorants. We found that MSAP promotes female mosquito attraction to human odor and enhances the sensitivity of the antennae to a variety of odorants. These findings suggest that MSAP is an important component of the mosquito olfactory system, which until now has gone completely unnoticed.},
keywords = {antenna, chemoreceptor, M3i, marois, mosquitoes, olfaction},
pubstate = {published},
tppubtype = {article}
}
Mosquitoes rely mainly on the sense of smell to decipher their environment and locate suitable food sources, hosts for blood feeding and oviposition sites. The molecular bases of olfaction involve multigenic families of olfactory proteins that have evolved to interact with a narrow set of odorants that are critical for survival. Understanding the complex interplay between diversified repertoires of olfactory proteins and ecologically-relevant odorant signals, which elicit important behaviors, is fundamental for the design of novel control strategies targeting the sense of smell of disease vector mosquitoes. Previously, large multigene families of odorant receptor and ionotropic receptor proteins, as well as a subset of odorant-binding proteins have been shown to mediate the selectivity and sensitivity of the mosquito olfactory system. In this study, we identify a mosquito-specific antennal protein (MSAP) gene as a novel molecular actor of odorant reception. MSAP is highly conserved across mosquito species and is transcribed at an extremely high level in female antennae. In order to understand its role in the mosquito olfactory system, we generated knockout mutant lines in Anopheles gambiae, and performed comparative analysis of behavioral and physiological responses to human-associated odorants. We found that MSAP promotes female mosquito attraction to human odor and enhances the sensitivity of the antennae to a variety of odorants. These findings suggest that MSAP is an important component of the mosquito olfactory system, which until now has gone completely unnoticed.
Klug Dennis, Gautier Amandine, Calvo Eric, Marois Eric, Blandin Stéphanie A.
The salivary protein Saglin facilitates efficient midgut colonization of Anopheles mosquitoes by malaria parasites Article de journal
Dans: Plos Pathogens, vol. 19, iss. 3, no. 3, 2023.
Résumé | Liens | BibTeX | Étiquettes: blandin, BLOOD, M3i, malarial parasites, marois, mosquitoes, Oocysts, Parasitic Diseases, Plasmodium, salivary glands, sporozoites
@article{Klug2023,
title = {The salivary protein Saglin facilitates efficient midgut colonization of Anopheles mosquitoes by malaria parasites},
author = {Dennis Klug and Amandine Gautier and Eric Calvo and Eric Marois and Stéphanie A. Blandin},
url = {https://doi.org/10.1371/journal.ppat.1010538},
doi = {10.1371/journal.ppat.1010538},
year = {2023},
date = {2023-03-02},
urldate = {2023-03-02},
booktitle = {Plos Pathogens},
journal = {Plos Pathogens},
volume = {19},
number = {3},
issue = {3},
abstract = {Female mosquitoes rely on blood feeding to acquire sufficient nutrients for egg development. Because of the importance of this process mosquitoes evolved salivary proteins with a broad range of functions acting as blood thinners, anti-coagulants and immunosuppressants. The effect of these proteins on the blood at the bite site directly influences the size of the blood bolus a female takes up in a given time frame. Both, time of feeding and bolus size, are important parameters for fecundity and survival. Recent studies have shown that a significant proportion of salivated proteins is re-ingested during feeding and becomes part of the blood meal. Here we investigated the salivary protein Saglin which has been previously suggested as putative receptor mediating malaria parasite entry into the salivary gland. By engineering a loss-of-function mutant in An. coluzzi we could show that the absence of Saglin impairs the development of parasite stages in the blood meal of the rodent malaria parasite P. berghei and the human malaria parasite P. falciparum lowering the parasite burden of subsequent stages and preventing efficient transmission at low infection densities. Furthermore, we could show that Saglin is present in the blood meal after feeding possibly indicating a previously overlooked parasite-vector interaction.},
keywords = {blandin, BLOOD, M3i, malarial parasites, marois, mosquitoes, Oocysts, Parasitic Diseases, Plasmodium, salivary glands, sporozoites},
pubstate = {published},
tppubtype = {article}
}
Female mosquitoes rely on blood feeding to acquire sufficient nutrients for egg development. Because of the importance of this process mosquitoes evolved salivary proteins with a broad range of functions acting as blood thinners, anti-coagulants and immunosuppressants. The effect of these proteins on the blood at the bite site directly influences the size of the blood bolus a female takes up in a given time frame. Both, time of feeding and bolus size, are important parameters for fecundity and survival. Recent studies have shown that a significant proportion of salivated proteins is re-ingested during feeding and becomes part of the blood meal. Here we investigated the salivary protein Saglin which has been previously suggested as putative receptor mediating malaria parasite entry into the salivary gland. By engineering a loss-of-function mutant in An. coluzzi we could show that the absence of Saglin impairs the development of parasite stages in the blood meal of the rodent malaria parasite P. berghei and the human malaria parasite P. falciparum lowering the parasite burden of subsequent stages and preventing efficient transmission at low infection densities. Furthermore, we could show that Saglin is present in the blood meal after feeding possibly indicating a previously overlooked parasite-vector interaction.
2020
Volohonsky Gloria, Paul-Gilloteaux Perrine, Stafkova Jitka, Soichot Julien, Salamero Jean, Levashina Elena A.
Kinetics of Plasmodium midgut invasion in Anopheles mosquitoes Article de journal
Dans: PLoS Pathog, vol. 16, no. 9, 2020, ISSN: 1553-7374.
Résumé | Liens | BibTeX | Étiquettes: Anopheles, M3i, Malaria, marois, midgut, Plasmodium
@article{volohonsky_plasmodium_2020,
title = {Kinetics of Plasmodium midgut invasion in Anopheles mosquitoes},
author = {Gloria Volohonsky and Perrine Paul-Gilloteaux and Jitka Stafkova and Julien Soichot and Jean Salamero and Elena A. Levashina
},
editor = {Kenneth D. Vernick, Institut Pasteur, FRANCE},
url = {https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1008739},
doi = {10.1371/journal.ppat.1008739},
issn = {1553-7374},
year = {2020},
date = {2020-09-18},
journal = {PLoS Pathog},
volume = {16},
number = {9},
abstract = {The traversal of the mosquito midgut cells is one of the critical stages in the life cycle of malaria parasites. Motile parasite forms, called ookinetes, traverse the midgut epithelium in a dynamic process which is not fully understood. Here, we harnessed transgenic reporters to track invasion of Plasmodium parasites in African and Indian mosquito species. We found important differences in parasite dynam- ics between the two Anopheles species and demonstrated a role of the mosquito comple- ment-like system in regulation of parasite invasion of the midgut cells.},
keywords = {Anopheles, M3i, Malaria, marois, midgut, Plasmodium},
pubstate = {published},
tppubtype = {article}
}
The traversal of the mosquito midgut cells is one of the critical stages in the life cycle of malaria parasites. Motile parasite forms, called ookinetes, traverse the midgut epithelium in a dynamic process which is not fully understood. Here, we harnessed transgenic reporters to track invasion of Plasmodium parasites in African and Indian mosquito species. We found important differences in parasite dynam- ics between the two Anopheles species and demonstrated a role of the mosquito comple- ment-like system in regulation of parasite invasion of the midgut cells.
2018
Olmo RP, Ferreira AGA, Izidoro-Toledo TC, Aguiar ERGR, de Faria IJS, de Souza KPR, Osório KP, Kuhn L, Hammann P, de Andrade EG, Todjro YM, Rocha MN, Leite THJF, Amadou SCG, Armache JN, Paro S, de Oliveira CD, Carvalho FD, Moreira LA, Marois E, Imler JL, Marques JT
Control of dengue virus in the midgut of Aedes aegypti by ectopic expression of the dsRNA-binding protein Loqs2 Article de journal
Dans: Nature Microbiology, vol. 3, no. 12, p. 1385-1393, 2018.
Résumé | Liens | BibTeX | Étiquettes: Aedes aegypti, Dengue, imler, M3i, marois, Marques, Zika
@article{Olmo_2018,
title = {Control of dengue virus in the midgut of Aedes aegypti by ectopic expression of the dsRNA-binding protein Loqs2 },
author = {RP Olmo and AGA Ferreira and TC Izidoro-Toledo and ERGR Aguiar and IJS de Faria and KPR de Souza and KP Osório and L Kuhn and P Hammann and EG de Andrade and YM Todjro and MN Rocha and THJF Leite and SCG Amadou and JN Armache and S Paro and CD de Oliveira and FD Carvalho and LA Moreira and E Marois and JL Imler and JT Marques},
url = {https://www.nature.com/articles/s41564-018-0268-6},
doi = {10.1038/s41564-018-0268-6},
year = {2018},
date = {2018-10-29},
journal = {Nature Microbiology},
volume = {3},
number = {12},
pages = {1385-1393},
abstract = {Dengue virus (DENV) is an arbovirus transmitted to humans by Aedes mosquitoes. In the insect vector, the small interfering RNA (siRNA) pathway is an important antiviral mechanism against DENV. However, it remains unclear when and where the siRNA pathway acts during the virus cycle. Here, we show that the siRNA pathway fails to efficiently silence DENV in the midgut of Aedes aegypti although it is essential to restrict systemic replication. Accumulation of DENV-derived siRNAs in the midgut reveals that impaired silencing results from a defect downstream of small RNA biogenesis. Notably, silencing triggered by endogenous and exogenous dsRNAs remained effective in the midgut where known components of the siRNA pathway, including the double-stranded RNA (dsRNA)-binding proteins Loquacious and r2d2, had normal expression levels. We identified an Aedes-specific paralogue of loquacious and r2d2, hereafter named loqs2, which is not expressed in the midgut. Loqs2 interacts with Loquacious and r2d2 and is required to control systemic replication of DENV and also Zika virus. Furthermore, ectopic expression of Loqs2 in the midgut of transgenic mosquitoes is sufficient to restrict DENV replication and dissemination. Together, our data reveal a mechanism of tissue-specific regulation of the mosquito siRNA pathway controlled by Loqs2. },
keywords = {Aedes aegypti, Dengue, imler, M3i, marois, Marques, Zika},
pubstate = {published},
tppubtype = {article}
}
Dengue virus (DENV) is an arbovirus transmitted to humans by Aedes mosquitoes. In the insect vector, the small interfering RNA (siRNA) pathway is an important antiviral mechanism against DENV. However, it remains unclear when and where the siRNA pathway acts during the virus cycle. Here, we show that the siRNA pathway fails to efficiently silence DENV in the midgut of Aedes aegypti although it is essential to restrict systemic replication. Accumulation of DENV-derived siRNAs in the midgut reveals that impaired silencing results from a defect downstream of small RNA biogenesis. Notably, silencing triggered by endogenous and exogenous dsRNAs remained effective in the midgut where known components of the siRNA pathway, including the double-stranded RNA (dsRNA)-binding proteins Loquacious and r2d2, had normal expression levels. We identified an Aedes-specific paralogue of loquacious and r2d2, hereafter named loqs2, which is not expressed in the midgut. Loqs2 interacts with Loquacious and r2d2 and is required to control systemic replication of DENV and also Zika virus. Furthermore, ectopic expression of Loqs2 in the midgut of transgenic mosquitoes is sufficient to restrict DENV replication and dissemination. Together, our data reveal a mechanism of tissue-specific regulation of the mosquito siRNA pathway controlled by Loqs2.
2017
Volohonsky Gloria, Hopp Ann-Katrin, Saenger Mélanie, Soichot Julien, Scholze Heidi, Boch Jens, Blandin Stéphanie A, Marois Eric
Transgenic Expression of the Anti-parasitic Factor TEP1 in the Malaria Mosquito Anopheles gambiae Article de journal
Dans: PLOS Pathogens, vol. 13, no. 1, p. e1006113, 2017, ISSN: 1553-7374.
Liens | BibTeX | Étiquettes: Anopheles gambiae, anti-parasitic factor, blandin, M3i, Malaria, marois, TEP1, transgenic
@article{volohonsky_transgenic_2017,
title = {Transgenic Expression of the Anti-parasitic Factor TEP1 in the Malaria Mosquito Anopheles gambiae},
author = {Gloria Volohonsky and Ann-Katrin Hopp and Mélanie Saenger and Julien Soichot and Heidi Scholze and Jens Boch and Stéphanie A Blandin and Eric Marois},
editor = {Kenneth D Vernick},
url = {http://dx.plos.org/10.1371/journal.ppat.1006113},
doi = {10.1371/journal.ppat.1006113},
issn = {1553-7374},
year = {2017},
date = {2017-01-01},
urldate = {2017-02-01},
journal = {PLOS Pathogens},
volume = {13},
number = {1},
pages = {e1006113},
keywords = {Anopheles gambiae, anti-parasitic factor, blandin, M3i, Malaria, marois, TEP1, transgenic},
pubstate = {published},
tppubtype = {article}
}
2015
Volohonsky Gloria, Terenzi Olivier, Soichot Julien, Naujoks Daniel A, Nolan Tony, Windbichler Nikolai, Kapps Delphine, Smidler Andie L, Vittu Anaïs, Costa Giulia, Steinert Stefanie, Levashina Elena A, Blandin Stéphanie A, Marois Eric
Tools for Anopheles gambiae Transgenesis Article de journal
Dans: G3 (Bethesda), vol. 5, no. 6, p. 1151-63, 2015.
Résumé | Liens | BibTeX | Étiquettes: Anopheles gambiae, bioinformatic, blandin, M3i, marois, transgenesis
@article{G2015,
title = {Tools for Anopheles gambiae Transgenesis},
author = {Gloria Volohonsky and Olivier Terenzi and Julien Soichot and Daniel A Naujoks and Tony Nolan and Nikolai Windbichler and Delphine Kapps and Andie L Smidler and Anaïs Vittu and Giulia Costa and Stefanie Steinert and Elena A Levashina and Stéphanie A Blandin and Eric Marois},
url = {http://www.ncbi.nlm.nih.gov/pubmed/25869647},
year = {2015},
date = {2015-04-13},
journal = {G3 (Bethesda)},
volume = {5},
number = {6},
pages = {1151-63},
abstract = {Transgenesis is an essential tool to investigate gene function and to introduce desired characters in laboratory organisms. Setting-up transgenesis in non-model organisms is challenging due to the diversity of biological life traits and due to knowledge gaps in genomic information. Some procedures will be broadly applicable to many organisms, and others have to be specifically developed for the target species. Transgenesis in disease vector mosquitoes has existed since the 2000s but has remained limited by the delicate biology of these insects. Here, we report a compilation of the transgenesis tools that we have designed for the malaria vector Anopheles gambiae, including new docking strains, convenient transgenesis plasmids, a puromycin resistance selection marker, mosquitoes expressing cre recombinase, and various reporter lines defining the activity of cloned promoters. This toolbox contributed to rendering transgenesis routine in this species and is now enabling the development of increasingly refined genetic manipulations such as targeted mutagenesis. Some of the reagents and procedures reported here are easily transferable to other nonmodel species, including other disease vector or agricultural pest insects.},
keywords = {Anopheles gambiae, bioinformatic, blandin, M3i, marois, transgenesis},
pubstate = {published},
tppubtype = {article}
}
Transgenesis is an essential tool to investigate gene function and to introduce desired characters in laboratory organisms. Setting-up transgenesis in non-model organisms is challenging due to the diversity of biological life traits and due to knowledge gaps in genomic information. Some procedures will be broadly applicable to many organisms, and others have to be specifically developed for the target species. Transgenesis in disease vector mosquitoes has existed since the 2000s but has remained limited by the delicate biology of these insects. Here, we report a compilation of the transgenesis tools that we have designed for the malaria vector Anopheles gambiae, including new docking strains, convenient transgenesis plasmids, a puromycin resistance selection marker, mosquitoes expressing cre recombinase, and various reporter lines defining the activity of cloned promoters. This toolbox contributed to rendering transgenesis routine in this species and is now enabling the development of increasingly refined genetic manipulations such as targeted mutagenesis. Some of the reagents and procedures reported here are easily transferable to other nonmodel species, including other disease vector or agricultural pest insects.
Hammond Andrew, Galizi Roberto, Kyrou Kyros, Simoni Alekos, Siniscalchi Carla, Katsanos Dimitris, Gribble Matthew, Baker Dean, Marois Eric, Russell Steven, Burt Austin, Windbichler Nikolai, Crisanti Andrea, Nolan Tony
A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae Article de journal
Dans: Nature Biotechnology, vol. 34, no. 1, p. 78–83, 2015, ISSN: 1087-0156, 1546-1696.
Liens | BibTeX | Étiquettes: M3i, marois
@article{hammond_crispr-cas9_2015,
title = {A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae},
author = {Andrew Hammond and Roberto Galizi and Kyros Kyrou and Alekos Simoni and Carla Siniscalchi and Dimitris Katsanos and Matthew Gribble and Dean Baker and Eric Marois and Steven Russell and Austin Burt and Nikolai Windbichler and Andrea Crisanti and Tony Nolan},
url = {http://www.nature.com/doifinder/10.1038/nbt.3439},
doi = {10.1038/nbt.3439},
issn = {1087-0156, 1546-1696},
year = {2015},
date = {2015-01-01},
urldate = {2016-01-26},
journal = {Nature Biotechnology},
volume = {34},
number = {1},
pages = {78--83},
keywords = {M3i, marois},
pubstate = {published},
tppubtype = {article}
}
2014
Gabrieli P, Marois Eric, Catteruccia Flaminia
Transgenic insects: techniques and applications Chapitre d'ouvrage
Dans: Benedicts, MQ (Ed.): Chapitre Sexual sterilization of mosquitoes, p. 188-207, CABI, 2014.
BibTeX | Étiquettes: insects, M3i, marois, sterile
@inbook{P2014,
title = {Transgenic insects: techniques and applications},
author = {P Gabrieli and Eric Marois and Flaminia Catteruccia},
editor = {MQ Benedicts},
year = {2014},
date = {2014-10-01},
pages = {188-207},
publisher = {CABI},
chapter = {Sexual sterilization of mosquitoes},
keywords = {insects, M3i, marois, sterile},
pubstate = {published},
tppubtype = {inbook}
}
Bernardini F, Galizi R, Menichelli M, Papathanos P A, Dritsou V, Marois Eric, Crisanti Andrea, Windbichler Nikolai
Site-specific genetic engineering of the Anopheles gambiae Y chromosome Article de journal
Dans: Proc Natl Acad Sci U S A., vol. 111, no. 21, p. 7600-5, 2014.
Résumé | Liens | BibTeX | Étiquettes: Anopheles gambiae, Biotechnology, M3i, marois, SIT, transgenesis
@article{F2014b,
title = {Site-specific genetic engineering of the Anopheles gambiae Y chromosome},
author = {F Bernardini and R Galizi and M Menichelli and P A Papathanos and V Dritsou and Eric Marois and Andrea Crisanti and Nikolai Windbichler},
url = {http://www.ncbi.nlm.nih.gov/pubmed/24821795},
year = {2014},
date = {2014-05-27},
journal = {Proc Natl Acad Sci U S A.},
volume = {111},
number = {21},
pages = {7600-5},
abstract = {Despite its function in sex determination and its role in driving genome evolution, the Y chromosome remains poorly understood in most species. Y chromosomes are gene-poor, repeat-rich and largely heterochromatic and therefore represent a difficult target for genetic engineering. The Y chromosome of the human malaria vector Anopheles gambiae appears to be involved in sex determination although very little is known about both its structure and function. Here, we characterize a transgenic strain of this mosquito species, obtained by transposon-mediated integration of a transgene construct onto the Y chromosome. Using meganuclease-induced homologous repair we introduce a site-specific recombination signal onto the Y chromosome and show that the resulting docking line can be used for secondary integration. To demonstrate its utility, we study the activity of a germ-line-specific promoter when located on the Y chromosome. We also show that Y-linked fluorescent transgenes allow automated sex separation of this important vector species, providing the means to generate large single-sex populations. Our findings will aid studies of sex chromosome function and enable the development of male-exclusive genetic traits for vector control.},
keywords = {Anopheles gambiae, Biotechnology, M3i, marois, SIT, transgenesis},
pubstate = {published},
tppubtype = {article}
}
Despite its function in sex determination and its role in driving genome evolution, the Y chromosome remains poorly understood in most species. Y chromosomes are gene-poor, repeat-rich and largely heterochromatic and therefore represent a difficult target for genetic engineering. The Y chromosome of the human malaria vector Anopheles gambiae appears to be involved in sex determination although very little is known about both its structure and function. Here, we characterize a transgenic strain of this mosquito species, obtained by transposon-mediated integration of a transgene construct onto the Y chromosome. Using meganuclease-induced homologous repair we introduce a site-specific recombination signal onto the Y chromosome and show that the resulting docking line can be used for secondary integration. To demonstrate its utility, we study the activity of a germ-line-specific promoter when located on the Y chromosome. We also show that Y-linked fluorescent transgenes allow automated sex separation of this important vector species, providing the means to generate large single-sex populations. Our findings will aid studies of sex chromosome function and enable the development of male-exclusive genetic traits for vector control.
2013
Smidler Andie L, Terenzi Olivier, Soichot Julien, Levashina Elena A, Marois Eric
Targeted Mutagenesis in the Malaria Mosquito Using TALE Nucleases Article de journal
Dans: PLoS One, vol. 8, no. 8, p. e74511, 2013.
Résumé | Liens | BibTeX | Étiquettes: M3i, marois, Mutagenesis, TALEN
@article{AL2013,
title = {Targeted Mutagenesis in the Malaria Mosquito Using TALE Nucleases},
author = {Andie L Smidler and Olivier Terenzi and Julien Soichot and Elena A Levashina and Eric Marois},
url = {http://www.ncbi.nlm.nih.gov/pubmed/23977401},
year = {2013},
date = {2013-08-15},
journal = {PLoS One},
volume = {8},
number = {8},
pages = {e74511},
abstract = {Anopheles gambiae, the main mosquito vector of human malaria, is a challenging organism to manipulate genetically. As a consequence, reverse genetics studies in this disease vector have been largely limited to RNA interference experiments. Here, we report the targeted disruption of the immunity gene TEP1 using transgenic expression of Transcription-Activator Like Effector Nucleases (TALENs), and the isolation of several TEP1 mutant A. gambiae lines. These mutations inhibited protein production and rendered TEP1 mutants hypersusceptible to Plasmodium berghei. The TALEN technology opens up new avenues for genetic analysis in this disease vector and may offer novel biotechnology-based approaches for malaria control.},
keywords = {M3i, marois, Mutagenesis, TALEN},
pubstate = {published},
tppubtype = {article}
}
Anopheles gambiae, the main mosquito vector of human malaria, is a challenging organism to manipulate genetically. As a consequence, reverse genetics studies in this disease vector have been largely limited to RNA interference experiments. Here, we report the targeted disruption of the immunity gene TEP1 using transgenic expression of Transcription-Activator Like Effector Nucleases (TALENs), and the isolation of several TEP1 mutant A. gambiae lines. These mutations inhibited protein production and rendered TEP1 mutants hypersusceptible to Plasmodium berghei. The TALEN technology opens up new avenues for genetic analysis in this disease vector and may offer novel biotechnology-based approaches for malaria control.
2012
Marois Eric, Scali C, Soichot Julien, Kappler Christine, Levashina Elena A, Catteruccia Flaminia
High-throughput sorting of mosquito larvae for laboratory studies and for future vector control interventions Article de journal
Dans: Malaria J., vol. 11, p. 302, 2012.
Résumé | Liens | BibTeX | Étiquettes: COPAS, M3i, marois, Sorting, transgenesis
@article{E2012,
title = {High-throughput sorting of mosquito larvae for laboratory studies and for future vector control interventions},
author = {Eric Marois and C Scali and Julien Soichot and Christine Kappler and Elena A Levashina and Flaminia Catteruccia},
url = {http://www.ncbi.nlm.nih.gov/pubmed/22929810},
year = {2012},
date = {2012-08-28},
journal = {Malaria J.},
volume = {11},
pages = {302},
abstract = {BACKGROUND: Mosquito transgenesis offers new promises for the genetic control of vector-borne infectious diseases such as malaria and dengue fever. Genetic control strategies require the release of large number of male mosquitoes into field populations, whether they are based on the use of sterile males (sterile insect technique, SIT) or on introducing genetic traits conferring refractoriness to disease transmission (population replacement). However, the current absence of high-throughput techniques for sorting different mosquito populations impairs the application of these control measures. METHODS: A method was developed to generate large mosquito populations of the desired sex and genotype. This method combines flow cytometry and the use of Anopheles gambiae transgenic lines that differentially express fluorescent markers in males and females. RESULTS: Fluorescence-assisted sorting allowed single-step isolation of homozygous transgenic mosquitoes from a mixed population. This method was also used to select wild-type males only with high efficiency and accuracy, a highly desirable tool for genetic control strategies where the release of transgenic individuals may be problematic. Importantly, sorted males showed normal mating ability compared to their unsorted brothers. CONCLUSIONS: The developed method will greatly facilitate both laboratory studies of mosquito vectorial capacity requiring high-throughput approaches and future field interventions in the fight against infectious disease vectors.},
keywords = {COPAS, M3i, marois, Sorting, transgenesis},
pubstate = {published},
tppubtype = {article}
}
BACKGROUND: Mosquito transgenesis offers new promises for the genetic control of vector-borne infectious diseases such as malaria and dengue fever. Genetic control strategies require the release of large number of male mosquitoes into field populations, whether they are based on the use of sterile males (sterile insect technique, SIT) or on introducing genetic traits conferring refractoriness to disease transmission (population replacement). However, the current absence of high-throughput techniques for sorting different mosquito populations impairs the application of these control measures. METHODS: A method was developed to generate large mosquito populations of the desired sex and genotype. This method combines flow cytometry and the use of Anopheles gambiae transgenic lines that differentially express fluorescent markers in males and females. RESULTS: Fluorescence-assisted sorting allowed single-step isolation of homozygous transgenic mosquitoes from a mixed population. This method was also used to select wild-type males only with high efficiency and accuracy, a highly desirable tool for genetic control strategies where the release of transgenic individuals may be problematic. Importantly, sorted males showed normal mating ability compared to their unsorted brothers. CONCLUSIONS: The developed method will greatly facilitate both laboratory studies of mosquito vectorial capacity requiring high-throughput approaches and future field interventions in the fight against infectious disease vectors.
2011
Marois Eric
The multifaceted mosquito anti-Plasmodium response Article de journal
Dans: Curr Opin Microbiol., vol. 14, no. 4, p. 429-35, 2011.
Résumé | Liens | BibTeX | Étiquettes: anti-Plasmodium response, M3i, marois
@article{E2011,
title = {The multifaceted mosquito anti-Plasmodium response},
author = {Eric Marois},
url = {http://www.ncbi.nlm.nih.gov/pubmed/21802348},
year = {2011},
date = {2011-07-27},
journal = {Curr Opin Microbiol.},
volume = {14},
number = {4},
pages = {429-35},
abstract = {Plasmodium development within its mosquito vector is an essential step in malaria transmission, as illustrated in world regions where malaria was successfully eradicated via vector control. The innate immune system of most mosquitoes is able to completely clear a Plasmodium infection, preventing parasite transmission to humans. Understanding the biological basis of this phenomenon is expected to inspire new strategies to curb malaria incidence in countries where vector control via insecticides is unpractical, or inefficient because insecticide resistance genes have spread across mosquito populations. Several aspects of mosquito biology that condition the success of the parasite in colonizing its vector begin to be understood at the molecular level, and a wealth of recently published data highlights the multifaceted nature of the mosquito response against parasite invasion. In this brief review, we attempt to provide an integrated view of the challenges faced by the parasite to successfully invade its mosquito host, and discuss the possible intervention strategies that could exploit this knowledge for the fight against human malaria.},
keywords = {anti-Plasmodium response, M3i, marois},
pubstate = {published},
tppubtype = {article}
}
Plasmodium development within its mosquito vector is an essential step in malaria transmission, as illustrated in world regions where malaria was successfully eradicated via vector control. The innate immune system of most mosquitoes is able to completely clear a Plasmodium infection, preventing parasite transmission to humans. Understanding the biological basis of this phenomenon is expected to inspire new strategies to curb malaria incidence in countries where vector control via insecticides is unpractical, or inefficient because insecticide resistance genes have spread across mosquito populations. Several aspects of mosquito biology that condition the success of the parasite in colonizing its vector begin to be understood at the molecular level, and a wealth of recently published data highlights the multifaceted nature of the mosquito response against parasite invasion. In this brief review, we attempt to provide an integrated view of the challenges faced by the parasite to successfully invade its mosquito host, and discuss the possible intervention strategies that could exploit this knowledge for the fight against human malaria.
2010
Rono Martin K, Whitten Miranda M, Oulad-Abdelghani M, Levashina Elena A, Marois Eric
The Major Yolk Protein Vitellogenin Interferes with the Anti-Plasmodium Response in the Malaria Mosquito Anopheles gambiae Article de journal
Dans: PLoS Biol., vol. 10, no. 7, p. e1000434, 2010.
Résumé | Liens | BibTeX | Étiquettes: anti-Plasmodium response, M3i, marois, vittelogenin
@article{MK2010,
title = {The Major Yolk Protein Vitellogenin Interferes with the Anti-Plasmodium Response in the Malaria Mosquito Anopheles gambiae},
author = {Martin K Rono and Miranda M Whitten and M Oulad-Abdelghani and Elena A Levashina and Eric Marois},
url = {http://www.ncbi.nlm.nih.gov/pubmed/20652016},
year = {2010},
date = {2010-07-20},
journal = {PLoS Biol.},
volume = {10},
number = {7},
pages = {e1000434},
abstract = {When taking a blood meal on a person infected with malaria, female Anopheles gambiae mosquitoes, the major vector of human malaria, acquire nutrients that will activate egg development (oogenesis) in their ovaries. Simultaneously, they infect themselves with the malaria parasite. On traversing the mosquito midgut epithelium, invading Plasmodium ookinetes are met with a potent innate immune response predominantly controlled by mosquito blood cells. Whether the concomitant processes of mosquito reproduction and immunity affect each other remains controversial. Here, we show that proteins that deliver nutrients to maturing mosquito oocytes interfere with the antiparasitic response. Lipophorin (Lp) and vitellogenin (Vg), two nutrient transport proteins, reduce the parasite-killing efficiency of the antiparasitic factor TEP1. In the absence of either nutrient transport protein, TEP1 binding to the ookinete surface becomes more efficient. We also show that Lp is required for the normal expression of Vg, and for later Plasmodium development at the oocyst stage. Furthermore, our results uncover an inhibitory role of the Cactus/REL1/REL2 signaling cassette in the expression of Vg, but not of Lp. We reveal molecular links that connect reproduction and immunity at several levels and provide a molecular basis for a long-suspected trade-off between these two processes.},
keywords = {anti-Plasmodium response, M3i, marois, vittelogenin},
pubstate = {published},
tppubtype = {article}
}
When taking a blood meal on a person infected with malaria, female Anopheles gambiae mosquitoes, the major vector of human malaria, acquire nutrients that will activate egg development (oogenesis) in their ovaries. Simultaneously, they infect themselves with the malaria parasite. On traversing the mosquito midgut epithelium, invading Plasmodium ookinetes are met with a potent innate immune response predominantly controlled by mosquito blood cells. Whether the concomitant processes of mosquito reproduction and immunity affect each other remains controversial. Here, we show that proteins that deliver nutrients to maturing mosquito oocytes interfere with the antiparasitic response. Lipophorin (Lp) and vitellogenin (Vg), two nutrient transport proteins, reduce the parasite-killing efficiency of the antiparasitic factor TEP1. In the absence of either nutrient transport protein, TEP1 binding to the ookinete surface becomes more efficient. We also show that Lp is required for the normal expression of Vg, and for later Plasmodium development at the oocyst stage. Furthermore, our results uncover an inhibitory role of the Cactus/REL1/REL2 signaling cassette in the expression of Vg, but not of Lp. We reveal molecular links that connect reproduction and immunity at several levels and provide a molecular basis for a long-suspected trade-off between these two processes.
2008
Blandin Stéphanie A, Marois Eric, Levashina Elena A
Antimalarial responses in Anopheles gambiae: from a complement-like protein to a complement-like pathway Article de journal
Dans: Cell Host Microbe., vol. 3, no. 6, p. 364-74, 2008.
Résumé | Liens | BibTeX | Étiquettes: blandin, M3i, marois, TEP1
@article{SA2008,
title = {Antimalarial responses in Anopheles gambiae: from a complement-like protein to a complement-like pathway},
author = {Stéphanie A Blandin and Eric Marois and Elena A Levashina},
url = {http://www.ncbi.nlm.nih.gov/pubmed/18541213},
year = {2008},
date = {2008-06-12},
journal = {Cell Host Microbe.},
volume = {3},
number = {6},
pages = {364-74},
abstract = {Malaria transmission between humans depends on the ability of Anopheles mosquitoes to support Plasmodium development. New perspectives in vector control are emerging from understanding the mosquito immune system, which plays critical roles in parasite recognition and killing. A number of factors controlling this process have been recently identified, and key among them is TEP1, a homolog of human complement factor C3 whose binding to the parasite surface targets it for subsequent killing. Here, we review our current knowledge of mosquito factors that respond to Plasmodium infection and elaborate on the activity and mode of action of the TEP1 complement-like pathway.},
keywords = {blandin, M3i, marois, TEP1},
pubstate = {published},
tppubtype = {article}
}
Malaria transmission between humans depends on the ability of Anopheles mosquitoes to support Plasmodium development. New perspectives in vector control are emerging from understanding the mosquito immune system, which plays critical roles in parasite recognition and killing. A number of factors controlling this process have been recently identified, and key among them is TEP1, a homolog of human complement factor C3 whose binding to the parasite surface targets it for subsequent killing. Here, we review our current knowledge of mosquito factors that respond to Plasmodium infection and elaborate on the activity and mode of action of the TEP1 complement-like pathway.
