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I2CT
Immunologie, Immunopathologie et Chimie Thérapeutique
Publications
2020
Ruiz Amalia, Lucherelli Matteo Andrea, Murera Diane, Lamon Delphine, Ménard-Moyon Cécilia, Bianco Alberto
Toxicological evaluation of highly water dispersible few-layer graphene in vivo Article de journal
Dans: Carbon, vol. 170, p. 347–360, 2020, ISSN: 00086223.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{ruiz_toxicological_2020,
title = {Toxicological evaluation of highly water dispersible few-layer graphene in vivo},
author = {Amalia Ruiz and Matteo Andrea Lucherelli and Diane Murera and Delphine Lamon and Cécilia Ménard-Moyon and Alberto Bianco},
url = {https://linkinghub.elsevier.com/retrieve/pii/S0008622320307855},
doi = {10.1016/j.carbon.2020.08.023},
issn = {00086223},
year = {2020},
date = {2020-12-01},
urldate = {2020-11-20},
journal = {Carbon},
volume = {170},
pages = {347--360},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Spenlé Caroline, Loustau Thomas, Murdamoothoo Devadarssen, Erne William, la Forest Divonne Stephanie Beghelli-de, Veber Romain, Petti Luciana, Bourdely Pierre, Mörgelin Matthias, Brauchle Eva-Maria, Cremel Gérard, Randrianarisoa Vony, Camara Abdouramane, Rekima Samah, Schaub Sebastian, Nouhen Kelly, Imhof Thomas, Hansen Uwe, Paul Nicodème, Carapito Raphael, Pythoud Nicolas, Hirschler Aurélie, Carapito Christine, Dumortier Hélène, Mueller Christopher G, Koch Manuel, Schenke-Layland Katja, Kon Shigeyuki, Sudaka Anne, Anjuère Fabienne, Obberghen-Schilling Ellen Van, Orend Gertraud
Tenascin-C Orchestrates an Immune-Suppressive Tumor Microenvironment in Oral Squamous Cell Carcinoma Article de journal
Dans: Cancer Immunology Research, vol. 8, no. 9, p. 1122–1138, 2020, ISSN: 2326-6074.
Résumé | Liens | BibTeX | Étiquettes: Dumortier, I2CT, Team-Dumortier, Team-Mueller
@article{spenle_tenascin-c_2020,
title = {Tenascin-C Orchestrates an Immune-Suppressive Tumor Microenvironment in Oral Squamous Cell Carcinoma},
author = {Caroline Spenlé and Thomas Loustau and Devadarssen Murdamoothoo and William Erne and Stephanie Beghelli-de la Forest Divonne and Romain Veber and Luciana Petti and Pierre Bourdely and Matthias Mörgelin and Eva-Maria Brauchle and Gérard Cremel and Vony Randrianarisoa and Abdouramane Camara and Samah Rekima and Sebastian Schaub and Kelly Nouhen and Thomas Imhof and Uwe Hansen and Nicodème Paul and Raphael Carapito and Nicolas Pythoud and Aurélie Hirschler and Christine Carapito and Hélène Dumortier and Christopher G Mueller and Manuel Koch and Katja Schenke-Layland and Shigeyuki Kon and Anne Sudaka and Fabienne Anjuère and Ellen Van Obberghen-Schilling and Gertraud Orend},
doi = {10.1158/2326-6066.CIR-20-0074},
issn = {2326-6074},
year = {2020},
date = {2020-09-01},
journal = {Cancer Immunology Research},
volume = {8},
number = {9},
pages = {1122--1138},
abstract = {Inherent immune suppression represents a major challenge in the treatment of human cancer. The extracellular matrix molecule tenascin-C promotes cancer by multiple mechanisms, yet the roles of tenascin-C in tumor immunity are incompletely understood. Using a 4NQO-induced oral squamous cell carcinoma (OSCC) model with abundant and absent tenascin-C, we demonstrated that tenascin-C enforced an immune-suppressive lymphoid stroma via CCL21/CCR7 signaling, leading to increased metastatic tumors. Through TLR4, tenascin-C increased expression of CCR7 in CD11c+ myeloid cells. By inducing CCL21 in lymphatic endothelial cells via integrin α9β1 and binding to CCL21, tenascin-C immobilized CD11c+ cells in the stroma. Inversion of the lymph node-to-tumor CCL21 gradient, recruitment of T regulatory cells, high expression of anti-inflammatory cytokines, and matrisomal components were hallmarks of the tenascin-C-instructed lymphoid stroma. Ablation of tenascin-C or CCR7 blockade inhibited the lymphoid immune-suppressive stromal properties, reducing tumor growth, progression, and metastasis. Thus, targeting CCR7 could be relevant in human head and neck tumors, as high tenascin-C expression and an immune-suppressive stroma correlate to poor patient survival.},
keywords = {Dumortier, I2CT, Team-Dumortier, Team-Mueller},
pubstate = {published},
tppubtype = {article}
}
Inherent immune suppression represents a major challenge in the treatment of human cancer. The extracellular matrix molecule tenascin-C promotes cancer by multiple mechanisms, yet the roles of tenascin-C in tumor immunity are incompletely understood. Using a 4NQO-induced oral squamous cell carcinoma (OSCC) model with abundant and absent tenascin-C, we demonstrated that tenascin-C enforced an immune-suppressive lymphoid stroma via CCL21/CCR7 signaling, leading to increased metastatic tumors. Through TLR4, tenascin-C increased expression of CCR7 in CD11c+ myeloid cells. By inducing CCL21 in lymphatic endothelial cells via integrin α9β1 and binding to CCL21, tenascin-C immobilized CD11c+ cells in the stroma. Inversion of the lymph node-to-tumor CCL21 gradient, recruitment of T regulatory cells, high expression of anti-inflammatory cytokines, and matrisomal components were hallmarks of the tenascin-C-instructed lymphoid stroma. Ablation of tenascin-C or CCR7 blockade inhibited the lymphoid immune-suppressive stromal properties, reducing tumor growth, progression, and metastasis. Thus, targeting CCR7 could be relevant in human head and neck tumors, as high tenascin-C expression and an immune-suppressive stroma correlate to poor patient survival.
Lin Hazel, Ji Ding‐Kun, Lucherelli Matteo Andrea, Reina Giacomo, Ippolito Stefano, Samorì Paolo, Bianco Alberto
Comparative Effects of Graphene and Molybdenum Disulfide on Human Macrophage Toxicity Article de journal
Dans: Small, vol. 16, no. 35, p. 2002194, 2020, ISSN: 1613-6810, 1613-6829.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{lin_comparative_2020,
title = {Comparative Effects of Graphene and Molybdenum Disulfide on Human Macrophage Toxicity},
author = {Hazel Lin and Ding‐Kun Ji and Matteo Andrea Lucherelli and Giacomo Reina and Stefano Ippolito and Paolo Samorì and Alberto Bianco},
url = {https://onlinelibrary.wiley.com/doi/10.1002/smll.202002194},
doi = {10.1002/smll.202002194},
issn = {1613-6810, 1613-6829},
year = {2020},
date = {2020-09-01},
urldate = {2020-11-20},
journal = {Small},
volume = {16},
number = {35},
pages = {2002194},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Newman Leon, Rodrigues Artur Filipe, Jasim Dhifaf A, Vacchi Isabella Anna, Ménard-Moyon Cécilia, Bianco Alberto, Bussy Cyrill, Kostarelos Kostas
Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide Nanosheets Article de journal
Dans: Cell Reports Physical Science, vol. 1, no. 9, p. 100176, 2020, ISSN: 26663864.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{newman_nose--brain_2020,
title = {Nose-to-Brain Translocation and Cerebral Biodegradation of Thin Graphene Oxide Nanosheets},
author = {Leon Newman and Artur Filipe Rodrigues and Dhifaf A Jasim and Isabella Anna Vacchi and Cécilia Ménard-Moyon and Alberto Bianco and Cyrill Bussy and Kostas Kostarelos},
url = {https://linkinghub.elsevier.com/retrieve/pii/S2666386420301879},
doi = {10.1016/j.xcrp.2020.100176},
issn = {26663864},
year = {2020},
date = {2020-09-01},
urldate = {2020-11-20},
journal = {Cell Reports Physical Science},
volume = {1},
number = {9},
pages = {100176},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Reina Giacomo, Peng Shiyuan, Jacquemin Lucas, Andrade Andrés Felipe, Bianco Alberto
Hard Nanomaterials in Time of Viral Pandemics Article de journal
Dans: ACS Nano, vol. 14, no. 8, p. 9364–9388, 2020, ISSN: 1936-0851, 1936-086X.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{reina_hard_2020,
title = {Hard Nanomaterials in Time of Viral Pandemics},
author = {Giacomo Reina and Shiyuan Peng and Lucas Jacquemin and Andrés Felipe Andrade and Alberto Bianco},
url = {https://pubs.acs.org/doi/10.1021/acsnano.0c04117},
doi = {10.1021/acsnano.0c04117},
issn = {1936-0851, 1936-086X},
year = {2020},
date = {2020-08-01},
urldate = {2020-11-20},
journal = {ACS Nano},
volume = {14},
number = {8},
pages = {9364--9388},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Pelin Marco, Lin Hazel, Gazzi Arianna, Sosa Silvio, Ponti Cristina, Ortega Amaya, Zurutuza Amaia, Vázquez Ester, Prato Maurizio, Tubaro Aurelia, Bianco Alberto
Partial Reversibility of the Cytotoxic Effect Induced by Graphene-Based Materials in Skin Keratinocytes Article de journal
Dans: Nanomaterials, vol. 10, no. 8, p. 1602, 2020, ISSN: 2079-4991.
Résumé | Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{pelin_partial_2020,
title = {Partial Reversibility of the Cytotoxic Effect Induced by Graphene-Based Materials in Skin Keratinocytes},
author = {Marco Pelin and Hazel Lin and Arianna Gazzi and Silvio Sosa and Cristina Ponti and Amaya Ortega and Amaia Zurutuza and Ester Vázquez and Maurizio Prato and Aurelia Tubaro and Alberto Bianco},
url = {https://www.mdpi.com/2079-4991/10/8/1602},
doi = {10.3390/nano10081602},
issn = {2079-4991},
year = {2020},
date = {2020-08-01},
urldate = {2020-11-20},
journal = {Nanomaterials},
volume = {10},
number = {8},
pages = {1602},
abstract = {In the frame of graphene-based material (GBM) hazard characterization, particular attention should be given to the cutaneous effects. Hence, this study investigates if HaCaT skin keratinocytes exposed to high concentrations of few-layer graphene (FLG) or partially dehydrated graphene oxide (d-GO) for a short time can recover from the cytotoxic insult, measured by means of cell viability, mitochondrial damage and oxidative stress, after GBM removal from the cell medium. When compared to 24 or 72 h continuous exposure, recovery experiments suggest that the cytotoxicity induced by 24 h exposure to GBM is only partially recovered after 48 h culture in GBM-free medium. This partial recovery, higher for FLG as compared to GO, is not mediated by autophagy and could be the consequence of GBM internalization into cells. The ability of GBMs to be internalized inside keratinocytes together with the partial reversibility of the cellular damage is important in assessing the risk associated with skin exposure to GBM-containing devices.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
In the frame of graphene-based material (GBM) hazard characterization, particular attention should be given to the cutaneous effects. Hence, this study investigates if HaCaT skin keratinocytes exposed to high concentrations of few-layer graphene (FLG) or partially dehydrated graphene oxide (d-GO) for a short time can recover from the cytotoxic insult, measured by means of cell viability, mitochondrial damage and oxidative stress, after GBM removal from the cell medium. When compared to 24 or 72 h continuous exposure, recovery experiments suggest that the cytotoxicity induced by 24 h exposure to GBM is only partially recovered after 48 h culture in GBM-free medium. This partial recovery, higher for FLG as compared to GO, is not mediated by autophagy and could be the consequence of GBM internalization into cells. The ability of GBMs to be internalized inside keratinocytes together with the partial reversibility of the cellular damage is important in assessing the risk associated with skin exposure to GBM-containing devices.
Rodrigues Artur Filipe, Newman Leon, Jasim Dhifaf, Mukherjee Sourav P, Wang Jun, Vacchi Isabella A, Ménard‐Moyon Cécilia, Bianco Alberto, Fadeel Bengt, Kostarelos Kostas, Bussy Cyrill
Size‐Dependent Pulmonary Impact of Thin Graphene Oxide Sheets in Mice: Toward Safe‐by‐Design Article de journal
Dans: Advanced Science, vol. 7, no. 12, p. 1903200, 2020, ISSN: 2198-3844, 2198-3844.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{rodrigues_sizedependent_2020,
title = {Size‐Dependent Pulmonary Impact of Thin Graphene Oxide Sheets in Mice: Toward Safe‐by‐Design},
author = {Artur Filipe Rodrigues and Leon Newman and Dhifaf Jasim and Sourav P Mukherjee and Jun Wang and Isabella A Vacchi and Cécilia Ménard‐Moyon and Alberto Bianco and Bengt Fadeel and Kostas Kostarelos and Cyrill Bussy},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.201903200},
doi = {10.1002/advs.201903200},
issn = {2198-3844, 2198-3844},
year = {2020},
date = {2020-06-01},
urldate = {2020-11-20},
journal = {Advanced Science},
volume = {7},
number = {12},
pages = {1903200},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Orecchioni Marco, Bordoni Valentina, Fuoco Claudia, Reina Giacomo, Lin Hazel, Zoccheddu Martina, Yilmazer Acelya, Zavan Barbara, Cesareni Gianni, Bedognetti Davide, Bianco Alberto, Delogu Lucia Gemma
Toward High‐Dimensional Single‐Cell Analysis of Graphene Oxide Biological Impact: Tracking on Immune Cells by Single‐Cell Mass Cytometry Article de journal
Dans: Small, vol. 16, no. 21, p. 2000123, 2020, ISSN: 1613-6810, 1613-6829.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{orecchioni_toward_2020,
title = {Toward High‐Dimensional Single‐Cell Analysis of Graphene Oxide Biological Impact: Tracking on Immune Cells by Single‐Cell Mass Cytometry},
author = {Marco Orecchioni and Valentina Bordoni and Claudia Fuoco and Giacomo Reina and Hazel Lin and Martina Zoccheddu and Acelya Yilmazer and Barbara Zavan and Gianni Cesareni and Davide Bedognetti and Alberto Bianco and Lucia Gemma Delogu},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/smll.202000123},
doi = {10.1002/smll.202000123},
issn = {1613-6810, 1613-6829},
year = {2020},
date = {2020-05-01},
urldate = {2020-11-20},
journal = {Small},
volume = {16},
number = {21},
pages = {2000123},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Vacchi Isabella A, Guo Shi, Raya Jésus, Bianco Alberto, Ménard‐Moyon Cécilia
Strategies for the Controlled Covalent Double Functionalization of Graphene Oxide Article de journal
Dans: Chemistry – A European Journal, vol. 26, no. 29, p. 6591–6598, 2020, ISSN: 0947-6539, 1521-3765.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{vacchi_strategies_2020,
title = {Strategies for the Controlled Covalent Double Functionalization of Graphene Oxide},
author = {Isabella A Vacchi and Shi Guo and Jésus Raya and Alberto Bianco and Cécilia Ménard‐Moyon},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/chem.201905785},
doi = {10.1002/chem.201905785},
issn = {0947-6539, 1521-3765},
year = {2020},
date = {2020-05-01},
urldate = {2020-11-20},
journal = {Chemistry – A European Journal},
volume = {26},
number = {29},
pages = {6591--6598},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Malanagahalli Sowmya, Murera Diane, Martín Cristina, Lin Hazel, Wadier Nadége, Dumortier Hélène, Vázquez Ester, Bianco Alberto
Few Layer Graphene Does Not Affect Cellular Homeostasis of Mouse Macrophages Article de journal
Dans: Nanomaterials (Basel, Switzerland), vol. 10, no. 2, 2020, ISSN: 2079-4991.
Résumé | Liens | BibTeX | Étiquettes: Autophagy, bone marrow derived macrophages, carbon nanomaterials, Dumortier, graphene, I2CT, primary immune cells, Team-Bianco, Team-Dumortier
@article{malanagahalli_few_2020,
title = {Few Layer Graphene Does Not Affect Cellular Homeostasis of Mouse Macrophages},
author = {Sowmya Malanagahalli and Diane Murera and Cristina Martín and Hazel Lin and Nadége Wadier and Hélène Dumortier and Ester Vázquez and Alberto Bianco},
doi = {10.3390/nano10020228},
issn = {2079-4991},
year = {2020},
date = {2020-01-01},
journal = {Nanomaterials (Basel, Switzerland)},
volume = {10},
number = {2},
abstract = {: Graphene-related materials (GRMs) are widely used in various applications due to their unique properties. A growing number of reports describe the impact of different carbon nanomaterials, including graphene oxide (GO), reduced GO (rGO), and carbon nanotubes (CNT), on immune cells, but there is still a very limited number of studies on graphene. In this work, we investigated the biological responses of few layer graphene (FLG) on mouse macrophages (bone marrow derived macrophages, BMDMs), which are part of the first line of defense in innate immunity. In particular, our paper describes our findings of short-term FLG treatment in BMDMs with a focus on observing material internalization and changes in general cell morphology. Subsequent investigation of cytotoxicity parameters showed that increasing doses of FLG did not hamper the viability of cells and did not trigger inflammatory responses. Basal level induced autophagic activity sufficed to maintain the cellular homeostasis of FLG treated cells. Our results shed light on the impact of FLG on primary macrophages and show that FLG does not elicit immunological responses leading to cell death.},
keywords = {Autophagy, bone marrow derived macrophages, carbon nanomaterials, Dumortier, graphene, I2CT, primary immune cells, Team-Bianco, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}
: Graphene-related materials (GRMs) are widely used in various applications due to their unique properties. A growing number of reports describe the impact of different carbon nanomaterials, including graphene oxide (GO), reduced GO (rGO), and carbon nanotubes (CNT), on immune cells, but there is still a very limited number of studies on graphene. In this work, we investigated the biological responses of few layer graphene (FLG) on mouse macrophages (bone marrow derived macrophages, BMDMs), which are part of the first line of defense in innate immunity. In particular, our paper describes our findings of short-term FLG treatment in BMDMs with a focus on observing material internalization and changes in general cell morphology. Subsequent investigation of cytotoxicity parameters showed that increasing doses of FLG did not hamper the viability of cells and did not trigger inflammatory responses. Basal level induced autophagic activity sufficed to maintain the cellular homeostasis of FLG treated cells. Our results shed light on the impact of FLG on primary macrophages and show that FLG does not elicit immunological responses leading to cell death.
Fujii R, Okubo K, Takashiba S, Bianco A, Nishina Y
Tailoring the interaction between graphene oxide and antibacterial pyridinium salts by terminal functional groups Article de journal
Dans: Carbon, vol. 160, p. 204–210, 2020, ISSN: 00086223.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{fujii_tailoring_2020,
title = {Tailoring the interaction between graphene oxide and antibacterial pyridinium salts by terminal functional groups},
author = {R Fujii and K Okubo and S Takashiba and A Bianco and Y Nishina},
url = {https://linkinghub.elsevier.com/retrieve/pii/S0008622319311972},
doi = {10.1016/j.carbon.2019.11.094},
issn = {00086223},
year = {2020},
date = {2020-01-01},
urldate = {2020-11-20},
journal = {Carbon},
volume = {160},
pages = {204--210},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Guo Shi, Nishina Yuta, Bianco Alberto, Ménard‐Moyon Cécilia
A Flexible Method for Covalent Double Functionalization of Graphene Oxide Article de journal
Dans: Angewandte Chemie International Edition, vol. 59, no. 4, p. 1542–1547, 2020, ISSN: 1433-7851, 1521-3773.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{guo_flexible_2020,
title = {A Flexible Method for Covalent Double Functionalization of Graphene Oxide},
author = {Shi Guo and Yuta Nishina and Alberto Bianco and Cécilia Ménard‐Moyon},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201913461},
doi = {10.1002/anie.201913461},
issn = {1433-7851, 1521-3773},
year = {2020},
date = {2020-01-01},
urldate = {2020-11-20},
journal = {Angewandte Chemie International Edition},
volume = {59},
number = {4},
pages = {1542--1547},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Wang Julie T -W, Klippstein Rebecca, Martincic Markus, Pach Elzbieta, Feldman Robert, Šefl Martin, Michel Yves, Asker Daniel, Sosabowski Jane K, Kalbac Martin, Ros Tatiana Da, Ménard-Moyon Cécilia, Bianco Alberto, Kyriakou Ioanna, Emfietzoglou Dimitris, Saccavini Jean-Claude, Ballesteros Belén, Al-Jamal Khuloud T, Tobias Gerard
Neutron Activated 153Sm Sealed in Carbon Nanocapsules for textitin Vivo Imaging and Tumor Radiotherapy Article de journal
Dans: ACS Nano, vol. 14, no. 1, p. 129–141, 2020, ISSN: 1936-0851, 1936-086X.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{wang_neutron_2020,
title = {Neutron Activated 153Sm Sealed in Carbon Nanocapsules for textitin Vivo Imaging and Tumor Radiotherapy},
author = {Julie T -W Wang and Rebecca Klippstein and Markus Martincic and Elzbieta Pach and Robert Feldman and Martin Šefl and Yves Michel and Daniel Asker and Jane K Sosabowski and Martin Kalbac and Tatiana Da Ros and Cécilia Ménard-Moyon and Alberto Bianco and Ioanna Kyriakou and Dimitris Emfietzoglou and Jean-Claude Saccavini and Belén Ballesteros and Khuloud T Al-Jamal and Gerard Tobias},
url = {https://pubs.acs.org/doi/10.1021/acsnano.9b04898},
doi = {10.1021/acsnano.9b04898},
issn = {1936-0851, 1936-086X},
year = {2020},
date = {2020-01-01},
urldate = {2020-11-20},
journal = {ACS Nano},
volume = {14},
number = {1},
pages = {129--141},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Backes Claudia, Abdelkader Amr M, Alonso Concepción, Andrieux-Ledier Amandine, Arenal Raul, Azpeitia Jon, Balakrishnan Nilanthy, Banszerus Luca, Barjon Julien, Bartali Ruben, Bellani Sebastiano, Berger Claire, Berger Reinhard, Ortega Bernal M M, Bernard Carlo, Beton Peter H, Beyer André, Bianco Alberto, Bøggild Peter, Bonaccorso Francesco, Barin Gabriela Borin, Botas Cristina, Bueno Rebeca A, Carriazo Daniel, Castellanos-Gomez Andres, Christian Meganne, Ciesielski Artur, Ciuk Tymoteusz, Cole Matthew T, Coleman Jonathan, Coletti Camilla, Crema Luigi, Cun Huanyao, Dasler Daniela, Fazio Domenico De, Díez Noel, Drieschner Simon, Duesberg Georg S, Fasel Roman, Feng Xinliang, Fina Alberto, Forti Stiven, Galiotis Costas, Garberoglio Giovanni, García Jorge M, Garrido Jose Antonio, Gibertini Marco, Gölzhäuser Armin, Gómez Julio, Greber Thomas, Hauke Frank, Hemmi Adrian, Hernandez-Rodriguez Irene, Hirsch Andreas, Hodge Stephen A, Huttel Yves, Jepsen Peter U, Jimenez Ignacio, Kaiser Ute, Kaplas Tommi, Kim HoKwon, Kis Andras, Papagelis Konstantinos, Kostarelos Kostas, Krajewska Aleksandra, Lee Kangho, Li Changfeng, Lipsanen Harri, Liscio Andrea, Lohe Martin R, Loiseau Annick, Lombardi Lucia, López Maria Francisca, Martin Oliver, Martín Cristina, Martínez Lidia, Martin-Gago Jose Angel, Martínez José Ignacio, Marzari Nicola, Mayoral Álvaro, McManus John, Melucci Manuela, Méndez Javier, Merino Cesar, Merino Pablo, Meyer Andreas P, Miniussi Elisa, Miseikis Vaidotas, Mishra Neeraj, Morandi Vittorio, Munuera Carmen, Muñoz Roberto, Nolan Hugo, Ortolani Luca, Ott Anna K, Palacio Irene, Palermo Vincenzo, Parthenios John, Pasternak Iwona, Patane Amalia, Prato Maurizio, Prevost Henri, Prudkovskiy Vladimir, Pugno Nicola, Rojo Teófilo, Rossi Antonio, Ruffieux Pascal, Samorì Paolo, Schué Léonard, Setijadi Eki, Seyller Thomas, Speranza Giorgio, Stampfer Christoph, Stenger Ingrid, Strupinski Wlodek, Svirko Yuri, Taioli Simone, Teo Kenneth B K, Testi Matteo, Tomarchio Flavia, Tortello Mauro, Treossi Emanuele, Turchanin Andrey, Vazquez Ester, Villaro Elvira, Whelan Patrick R, Xia Zhenyuan, Yakimova Rositza, Yang Sheng, Yazdi Reza G, Yim Chanyoung, Yoon Duhee, Zhang Xianghui, Zhuang Xiaodong, Colombo Luigi, Ferrari Andrea C, Garcia-Hernandez Mar
Production and processing of graphene and related materials Article de journal
Dans: 2D Materials, vol. 7, no. 2, p. 022001, 2020, ISSN: 2053-1583.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{backes_production_2020,
title = {Production and processing of graphene and related materials},
author = {Claudia Backes and Amr M Abdelkader and Concepción Alonso and Amandine Andrieux-Ledier and Raul Arenal and Jon Azpeitia and Nilanthy Balakrishnan and Luca Banszerus and Julien Barjon and Ruben Bartali and Sebastiano Bellani and Claire Berger and Reinhard Berger and Bernal M M Ortega and Carlo Bernard and Peter H Beton and André Beyer and Alberto Bianco and Peter Bøggild and Francesco Bonaccorso and Gabriela Borin Barin and Cristina Botas and Rebeca A Bueno and Daniel Carriazo and Andres Castellanos-Gomez and Meganne Christian and Artur Ciesielski and Tymoteusz Ciuk and Matthew T Cole and Jonathan Coleman and Camilla Coletti and Luigi Crema and Huanyao Cun and Daniela Dasler and Domenico De Fazio and Noel Díez and Simon Drieschner and Georg S Duesberg and Roman Fasel and Xinliang Feng and Alberto Fina and Stiven Forti and Costas Galiotis and Giovanni Garberoglio and Jorge M García and Jose Antonio Garrido and Marco Gibertini and Armin Gölzhäuser and Julio Gómez and Thomas Greber and Frank Hauke and Adrian Hemmi and Irene Hernandez-Rodriguez and Andreas Hirsch and Stephen A Hodge and Yves Huttel and Peter U Jepsen and Ignacio Jimenez and Ute Kaiser and Tommi Kaplas and HoKwon Kim and Andras Kis and Konstantinos Papagelis and Kostas Kostarelos and Aleksandra Krajewska and Kangho Lee and Changfeng Li and Harri Lipsanen and Andrea Liscio and Martin R Lohe and Annick Loiseau and Lucia Lombardi and Maria Francisca López and Oliver Martin and Cristina Martín and Lidia Martínez and Jose Angel Martin-Gago and José Ignacio Martínez and Nicola Marzari and Álvaro Mayoral and John McManus and Manuela Melucci and Javier Méndez and Cesar Merino and Pablo Merino and Andreas P Meyer and Elisa Miniussi and Vaidotas Miseikis and Neeraj Mishra and Vittorio Morandi and Carmen Munuera and Roberto Muñoz and Hugo Nolan and Luca Ortolani and Anna K Ott and Irene Palacio and Vincenzo Palermo and John Parthenios and Iwona Pasternak and Amalia Patane and Maurizio Prato and Henri Prevost and Vladimir Prudkovskiy and Nicola Pugno and Teófilo Rojo and Antonio Rossi and Pascal Ruffieux and Paolo Samorì and Léonard Schué and Eki Setijadi and Thomas Seyller and Giorgio Speranza and Christoph Stampfer and Ingrid Stenger and Wlodek Strupinski and Yuri Svirko and Simone Taioli and Kenneth B K Teo and Matteo Testi and Flavia Tomarchio and Mauro Tortello and Emanuele Treossi and Andrey Turchanin and Ester Vazquez and Elvira Villaro and Patrick R Whelan and Zhenyuan Xia and Rositza Yakimova and Sheng Yang and Reza G Yazdi and Chanyoung Yim and Duhee Yoon and Xianghui Zhang and Xiaodong Zhuang and Luigi Colombo and Andrea C Ferrari and Mar Garcia-Hernandez},
url = {https://iopscience.iop.org/article/10.1088/2053-1583/ab1e0a},
doi = {10.1088/2053-1583/ab1e0a},
issn = {2053-1583},
year = {2020},
date = {2020-01-01},
urldate = {2020-11-20},
journal = {2D Materials},
volume = {7},
number = {2},
pages = {022001},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Dinesh Bhimareddy, Medelin Manuela, Scaini Denis, Faccini Federica Lareno, Quici Federica, Ballerini Laura, Bianco Alberto
Hybrid Interfaces Made of Nanotubes and Backbone-Altered Dipeptides Tune Neuronal Network Architecture Article de journal
Dans: ACS Chemical Neuroscience, vol. 11, no. 2, p. 162–172, 2020, ISSN: 1948-7193, 1948-7193.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{dinesh_hybrid_2020,
title = {Hybrid Interfaces Made of Nanotubes and Backbone-Altered Dipeptides Tune Neuronal Network Architecture},
author = {Bhimareddy Dinesh and Manuela Medelin and Denis Scaini and Federica Lareno Faccini and Federica Quici and Laura Ballerini and Alberto Bianco},
url = {https://pubs.acs.org/doi/10.1021/acschemneuro.9b00522},
doi = {10.1021/acschemneuro.9b00522},
issn = {1948-7193, 1948-7193},
year = {2020},
date = {2020-01-01},
urldate = {2020-11-20},
journal = {ACS Chemical Neuroscience},
volume = {11},
number = {2},
pages = {162--172},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Aloisi Adriano, Christensen Niels Johan, Sørensen Kasper K, Guilbaud-Chéreau Chloé, Jensen Knud J, Bianco Alberto
Dans: European Journal of Organic Chemistry, vol. 2020, no. 7, p. 815–820, 2020, ISSN: 1434193X.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{aloisi_synthesis_2020,
title = {Synthesis and Characterization of Adamantane-Containing Heteropeptides with a Chirality Switch: Synthesis and Characterization of Adamantane-Containing Heteropeptides with a Chirality Switch},
author = {Adriano Aloisi and Niels Johan Christensen and Kasper K Sørensen and Chloé Guilbaud-Chéreau and Knud J Jensen and Alberto Bianco},
url = {http://doi.wiley.com/10.1002/ejoc.201901666},
doi = {10.1002/ejoc.201901666},
issn = {1434193X},
year = {2020},
date = {2020-01-01},
urldate = {2020-11-20},
journal = {European Journal of Organic Chemistry},
volume = {2020},
number = {7},
pages = {815--820},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Wang Julie Tzu-Wen, Spinato Cinzia, Klippstein Rebecca, Costa Pedro Miguel, Martincic Markus, Pach Elzbieta, de Garibay Aritz Perez Ruiz, Ménard-Moyon Cécilia, Feldman Robert, Michel Yves, Šefl Martin, Kyriakou Ioanna, Emfietzoglou Dimitris, Saccavini Jean-Claude, Ballesteros Belén, Tobias Gerard, Bianco Alberto, Al-Jamal Khuloud T
Neutron-irradiated antibody-functionalised carbon nanocapsules for targeted cancer radiotherapy Article de journal
Dans: Carbon, vol. 162, p. 410–422, 2020, ISSN: 00086223.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{wang_neutron-irradiated_2020,
title = {Neutron-irradiated antibody-functionalised carbon nanocapsules for targeted cancer radiotherapy},
author = {Julie Tzu-Wen Wang and Cinzia Spinato and Rebecca Klippstein and Pedro Miguel Costa and Markus Martincic and Elzbieta Pach and Aritz Perez Ruiz de Garibay and Cécilia Ménard-Moyon and Robert Feldman and Yves Michel and Martin Šefl and Ioanna Kyriakou and Dimitris Emfietzoglou and Jean-Claude Saccavini and Belén Ballesteros and Gerard Tobias and Alberto Bianco and Khuloud T Al-Jamal},
url = {https://linkinghub.elsevier.com/retrieve/pii/S0008622320302062},
doi = {10.1016/j.carbon.2020.02.060},
issn = {00086223},
year = {2020},
date = {2020-01-01},
urldate = {2020-11-20},
journal = {Carbon},
volume = {162},
pages = {410--422},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Bianco Alberto, Chen Yuan, Frackowiak Elzbieta, Holzinger Michael, Koratkar Nikhil, Meunier Vincent, Mikhailovsky Sergey, Strano Michael, Tascon Juan M D, Terrones Mauricio
Carbon science perspective in 2020: Current research and future challenges Article de journal
Dans: Carbon, vol. 161, p. 373–391, 2020, ISSN: 00086223.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{bianco_carbon_2020,
title = {Carbon science perspective in 2020: Current research and future challenges},
author = {Alberto Bianco and Yuan Chen and Elzbieta Frackowiak and Michael Holzinger and Nikhil Koratkar and Vincent Meunier and Sergey Mikhailovsky and Michael Strano and Juan M D Tascon and Mauricio Terrones},
url = {https://linkinghub.elsevier.com/retrieve/pii/S0008622320300622},
doi = {10.1016/j.carbon.2020.01.055},
issn = {00086223},
year = {2020},
date = {2020-01-01},
urldate = {2020-11-20},
journal = {Carbon},
volume = {161},
pages = {373--391},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Lucherelli Matteo Andrea, Yu Yue, Reina Giacomo, Abellán Gonzalo, Miyako Eijiro, Bianco Alberto
Rational Chemical Multifunctionalization of Graphene Interface Enhances Targeted Cancer Therapy Article de journal
Dans: Angewandte Chemie International Edition, vol. 59, no. 33, p. 14034–14039, 2020, ISSN: 1433-7851, 1521-3773.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{lucherelli_rational_2020,
title = {Rational Chemical Multifunctionalization of Graphene Interface Enhances Targeted Cancer Therapy},
author = {Matteo Andrea Lucherelli and Yue Yu and Giacomo Reina and Gonzalo Abellán and Eijiro Miyako and Alberto Bianco},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201916112},
doi = {10.1002/anie.201916112},
issn = {1433-7851, 1521-3773},
year = {2020},
date = {2020-01-01},
urldate = {2020-11-20},
journal = {Angewandte Chemie International Edition},
volume = {59},
number = {33},
pages = {14034--14039},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Soltani Rym, Guo Shi, Bianco Alberto, Ménard‐Moyon Cécilia
Carbon Nanomaterials Applied for the Treatment of Inflammatory Diseases: Preclinical Evidence Article de journal
Dans: Advanced Therapeutics, vol. 3, no. 9, p. 2000051, 2020, ISSN: 2366-3987, 2366-3987.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{soltani_carbon_2020,
title = {Carbon Nanomaterials Applied for the Treatment of Inflammatory Diseases: Preclinical Evidence},
author = {Rym Soltani and Shi Guo and Alberto Bianco and Cécilia Ménard‐Moyon},
url = {https://onlinelibrary.wiley.com/doi/10.1002/adtp.202000051},
doi = {10.1002/adtp.202000051},
issn = {2366-3987, 2366-3987},
year = {2020},
date = {2020-01-01},
urldate = {2020-11-20},
journal = {Advanced Therapeutics},
volume = {3},
number = {9},
pages = {2000051},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Gazzi Arianna, Fusco Laura, Orecchioni Marco, Ferrari Silvia, Franzoni Giulia, Yan Stephen J, Rieckher Matthias, Peng Guotao, Lucherelli Matteo Andrea, Vacchi Isabella Anna, Chau Ngoc Do Quyen, Criado Alejandro, Istif Akcan, Mancino Donato, Dominguez Antonio, Eckert Hagen, Vázquez Ester, Ros Tatiana Da, Nicolussi Paola, Palermo Vincenzo, Schumacher Björn, Cuniberti Gianaurelio, Mai Yiyong, Clementi Cecilia, Pasquali Matteo, Feng Xinliang, Kostarelos Kostas, Yilmazer Acelya, Bedognetti Davide, Fadeel Bengt, Prato Maurizio, Bianco Alberto, Delogu Lucia Gemma
Graphene, other carbon nanomaterials and the immune system: toward nanoimmunity-by-design Article de journal
Dans: Journal of Physics: Materials, vol. 3, no. 3, p. 034009, 2020, ISSN: 2515-7639.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{gazzi_graphene_2020,
title = {Graphene, other carbon nanomaterials and the immune system: toward nanoimmunity-by-design},
author = {Arianna Gazzi and Laura Fusco and Marco Orecchioni and Silvia Ferrari and Giulia Franzoni and Stephen J Yan and Matthias Rieckher and Guotao Peng and Matteo Andrea Lucherelli and Isabella Anna Vacchi and Ngoc Do Quyen Chau and Alejandro Criado and Akcan Istif and Donato Mancino and Antonio Dominguez and Hagen Eckert and Ester Vázquez and Tatiana Da Ros and Paola Nicolussi and Vincenzo Palermo and Björn Schumacher and Gianaurelio Cuniberti and Yiyong Mai and Cecilia Clementi and Matteo Pasquali and Xinliang Feng and Kostas Kostarelos and Acelya Yilmazer and Davide Bedognetti and Bengt Fadeel and Maurizio Prato and Alberto Bianco and Lucia Gemma Delogu},
url = {https://iopscience.iop.org/article/10.1088/2515-7639/ab9317},
doi = {10.1088/2515-7639/ab9317},
issn = {2515-7639},
year = {2020},
date = {2020-01-01},
urldate = {2020-11-20},
journal = {Journal of Physics: Materials},
volume = {3},
number = {3},
pages = {034009},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Raya Jésus, Bianco Alberto, Hirschinger Jérôme
Dans: Physical Chemistry Chemical Physics, vol. 22, no. 21, p. 12209–12227, 2020, ISSN: 1463-9076, 1463-9084.
Résumé | Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{raya_kinetics_2020,
title = {Kinetics of 1H–13C multiple-contact multiple-contact cross-polarization as a powerful tool to determine the structure and dynamics of complex materials: application to graphene oxide},
author = {Jésus Raya and Alberto Bianco and Jérôme Hirschinger},
url = {http://xlink.rsc.org/?DOI=D0CP00454E},
doi = {10.1039/D0CP00454E},
issn = {1463-9076, 1463-9084},
year = {2020},
date = {2020-01-01},
urldate = {2020-11-20},
journal = {Physical Chemistry Chemical Physics},
volume = {22},
number = {21},
pages = {12209--12227},
abstract = {Structural and dynamical details are probed by kinetics of 1 H– 13 C single- and multiple-contact cross-polarization in graphene oxide. , Hartmann–Hahn cross-polarization (HHCP) is the most widely used solid-state NMR technique to enhance the magnetization of dilute spins from abundant spins. Furthermore, as the kinetics of CP depends on dipolar interactions, it contains valuable information on molecular structure and dynamics. In this work, analytical solutions are derived for the kinetics of HHCP and multiple-contact CP (MC-CP) using both classical and non-classical spin-coupling models including the effects of molecular dynamics and several 1 H, 13 C relaxation and 1 H– 13 C CP experiments are performed in graphene oxide (GO). HHCP is found to be inefficient in our GO sample due to very fast 1 H T 1ρ relaxation. By contrast, the MC-CP technique which alleviates most of the magnetization loss by 1 H T 1ρ relaxation leads to a much larger polarization transfer efficiency reducing the measuring time by an order of magnitude. A detailed analysis of the HHCP and MC-CP kinetics indicates the existence of at least two different kinds of hydroxyl (C–OH) functional groups in GO, the major fraction (∼90%) of these groups being in the unusual “slow CP regime” in which the rate of 1 H T 1ρ relaxation is fast compared to the rate of cross-polarization. This 13 C signal component is attributed to mobile C–OH groups interacting preferentially with fast-relaxing water molecules while the remaining carbons (∼10%) in the usual “fast CP regime” are assigned to C–OH groups involved in hydrogen bonding with neighboring hydroxyl and/or epoxy groups.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Structural and dynamical details are probed by kinetics of 1 H– 13 C single- and multiple-contact cross-polarization in graphene oxide. , Hartmann–Hahn cross-polarization (HHCP) is the most widely used solid-state NMR technique to enhance the magnetization of dilute spins from abundant spins. Furthermore, as the kinetics of CP depends on dipolar interactions, it contains valuable information on molecular structure and dynamics. In this work, analytical solutions are derived for the kinetics of HHCP and multiple-contact CP (MC-CP) using both classical and non-classical spin-coupling models including the effects of molecular dynamics and several 1 H, 13 C relaxation and 1 H– 13 C CP experiments are performed in graphene oxide (GO). HHCP is found to be inefficient in our GO sample due to very fast 1 H T 1ρ relaxation. By contrast, the MC-CP technique which alleviates most of the magnetization loss by 1 H T 1ρ relaxation leads to a much larger polarization transfer efficiency reducing the measuring time by an order of magnitude. A detailed analysis of the HHCP and MC-CP kinetics indicates the existence of at least two different kinds of hydroxyl (C–OH) functional groups in GO, the major fraction (∼90%) of these groups being in the unusual “slow CP regime” in which the rate of 1 H T 1ρ relaxation is fast compared to the rate of cross-polarization. This 13 C signal component is attributed to mobile C–OH groups interacting preferentially with fast-relaxing water molecules while the remaining carbons (∼10%) in the usual “fast CP regime” are assigned to C–OH groups involved in hydrogen bonding with neighboring hydroxyl and/or epoxy groups.
Ji Ding-Kun, Reina Giacomo, Guo Shi, Eredia Matilde, Samorì Paolo, Ménard-Moyon Cécilia, Bianco Alberto
Controlled functionalization of carbon nanodots for targeted intracellular production of reactive oxygen species Article de journal
Dans: Nanoscale Horizons, vol. 5, no. 8, p. 1240–1249, 2020, ISSN: 2055-6756, 2055-6764.
Résumé | Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{ji_controlled_2020,
title = {Controlled functionalization of carbon nanodots for targeted intracellular production of reactive oxygen species},
author = {Ding-Kun Ji and Giacomo Reina and Shi Guo and Matilde Eredia and Paolo Samorì and Cécilia Ménard-Moyon and Alberto Bianco},
url = {http://xlink.rsc.org/?DOI=D0NH00300J},
doi = {10.1039/D0NH00300J},
issn = {2055-6756, 2055-6764},
year = {2020},
date = {2020-01-01},
urldate = {2020-11-20},
journal = {Nanoscale Horizons},
volume = {5},
number = {8},
pages = {1240--1249},
abstract = {Multifunctional carbon nanodots with deep-red emission were prepared. These carbon nanodots are suitable for targeted intracellular production of reactive oxygen species by laser irradiation leading to efficient cancer cell death. , Controlled intracellular release of exogenous reactive oxygen species (ROS) is an innovative and efficient strategy for cancer treatment. Well-designed materials, which can produce ROS in targeted cells, minimizing side effects, still need to be explored as new generation nanomedicines. Here, red-emissive carbon nanodots (CNDs) with intrinsic theranostic properties are devised, and further modified with folic acid (FA) ligand through a controlled covalent functionalization for targeted cell imaging and intracellular production of ROS. We demonstrated that covalent functionalization is an effective strategy to prevent the aggregation of the dots, leading to superior colloidal stability, enhanced luminescence and ROS generation. Indeed, the functional nanodots possess a deep-red emission and good dispersibility under physiological conditions. Importantly, they show excellent targeting properties and generation of high levels of ROS under 660 nm laser irradiation, leading to efficient cell death. These unique properties enable FA-modified carbon nanodots to act as a multifunctional nanoplatform for simultaneous targeted imaging and efficient photodynamic therapy to induce cancer cell death.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Multifunctional carbon nanodots with deep-red emission were prepared. These carbon nanodots are suitable for targeted intracellular production of reactive oxygen species by laser irradiation leading to efficient cancer cell death. , Controlled intracellular release of exogenous reactive oxygen species (ROS) is an innovative and efficient strategy for cancer treatment. Well-designed materials, which can produce ROS in targeted cells, minimizing side effects, still need to be explored as new generation nanomedicines. Here, red-emissive carbon nanodots (CNDs) with intrinsic theranostic properties are devised, and further modified with folic acid (FA) ligand through a controlled covalent functionalization for targeted cell imaging and intracellular production of ROS. We demonstrated that covalent functionalization is an effective strategy to prevent the aggregation of the dots, leading to superior colloidal stability, enhanced luminescence and ROS generation. Indeed, the functional nanodots possess a deep-red emission and good dispersibility under physiological conditions. Importantly, they show excellent targeting properties and generation of high levels of ROS under 660 nm laser irradiation, leading to efficient cell death. These unique properties enable FA-modified carbon nanodots to act as a multifunctional nanoplatform for simultaneous targeted imaging and efficient photodynamic therapy to induce cancer cell death.
Guo Shi, Raya Jésus, Ji Dingkun, Nishina Yuta, Ménard-Moyon Cécilia, Bianco Alberto
Is carboxylation an efficient method for graphene oxide functionalization? Article de journal
Dans: Nanoscale Advances, vol. 2, no. 9, p. 4085–4092, 2020, ISSN: 2516-0230.
Résumé | Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{guo_is_2020,
title = {Is carboxylation an efficient method for graphene oxide functionalization?},
author = {Shi Guo and Jésus Raya and Dingkun Ji and Yuta Nishina and Cécilia Ménard-Moyon and Alberto Bianco},
url = {http://xlink.rsc.org/?DOI=D0NA00561D},
doi = {10.1039/D0NA00561D},
issn = {2516-0230},
year = {2020},
date = {2020-01-01},
urldate = {2020-11-20},
journal = {Nanoscale Advances},
volume = {2},
number = {9},
pages = {4085--4092},
abstract = {We investigated the carboxylation of graphene oxide using chloroacetic acid and different amounts of NaOH. Increase of carboxyl groups was observed only at high amounts of NaOH, but partial reduction attenuates the yield of further functionalization. , Graphene oxide (GO) is one of the most popular materials applied in different research areas thanks to its unique properties. The application of GO requires well-designed protocols to introduce different functionalities on its surface, exploiting the oxygenated groups already present. Due to the complex and unstable chemical environment on the GO surface, it is recommended to perform the functionalization under mild conditions. The carboxylation of GO is a widely used method to introduce additional carboxylic acids, which could be further modified through amidation or esterification reactions. The strategy already reported in the literature requires harsh conditions (excess amount of sodium hydroxide). GO is readily reduced under basic conditions, but the reduction of GO during the carboxylation is barely studied. In this work, we performed the carboxylation using chloroacetic acid with different amounts of sodium hydroxide and characterized the functionalized GO with various techniques. The carboxylated GO was exploited to develop a double functionalization approach combining an epoxide ring opening reaction and an amidation. The results showed that strong basic conditions were necessary to derivatize GO. Nevertheless, these conditions resulted in a partial reduction of GO and some functionalities on GO were removed during the reaction, thus reducing the total efficiency of the functionalization in comparison to an epoxide ring opening reaction, indicating that carboxylation is not an efficient approach for the functionalization of GO.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
We investigated the carboxylation of graphene oxide using chloroacetic acid and different amounts of NaOH. Increase of carboxyl groups was observed only at high amounts of NaOH, but partial reduction attenuates the yield of further functionalization. , Graphene oxide (GO) is one of the most popular materials applied in different research areas thanks to its unique properties. The application of GO requires well-designed protocols to introduce different functionalities on its surface, exploiting the oxygenated groups already present. Due to the complex and unstable chemical environment on the GO surface, it is recommended to perform the functionalization under mild conditions. The carboxylation of GO is a widely used method to introduce additional carboxylic acids, which could be further modified through amidation or esterification reactions. The strategy already reported in the literature requires harsh conditions (excess amount of sodium hydroxide). GO is readily reduced under basic conditions, but the reduction of GO during the carboxylation is barely studied. In this work, we performed the carboxylation using chloroacetic acid with different amounts of sodium hydroxide and characterized the functionalized GO with various techniques. The carboxylated GO was exploited to develop a double functionalization approach combining an epoxide ring opening reaction and an amidation. The results showed that strong basic conditions were necessary to derivatize GO. Nevertheless, these conditions resulted in a partial reduction of GO and some functionalities on GO were removed during the reaction, thus reducing the total efficiency of the functionalization in comparison to an epoxide ring opening reaction, indicating that carboxylation is not an efficient approach for the functionalization of GO.
Ma Baojin, Martín Cristina, Kurapati Rajendra, Bianco Alberto
Degradation-by-design: how chemical functionalization enhances the biodegradability and safety of 2D materials Article de journal
Dans: Chemical Society Reviews, vol. 49, no. 17, p. 6224–6247, 2020, ISSN: 0306-0012, 1460-4744.
Résumé | Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{ma_degradation-by-design_2020,
title = {Degradation-by-design: how chemical functionalization enhances the biodegradability and safety of 2D materials},
author = {Baojin Ma and Cristina Martín and Rajendra Kurapati and Alberto Bianco},
url = {http://xlink.rsc.org/?DOI=C9CS00822E},
doi = {10.1039/C9CS00822E},
issn = {0306-0012, 1460-4744},
year = {2020},
date = {2020-01-01},
urldate = {2020-11-20},
journal = {Chemical Society Reviews},
volume = {49},
number = {17},
pages = {6224--6247},
abstract = {A large number of graphene and other 2D materials are currently explored for the development of new technologies. The assessment of their biodegradability is one of the fundamental aspects for their safe application. , A large number of graphene and other 2D materials are currently used for the development of new technologies, increasingly entering different industrial sectors. Interrogating the impact of such 2D materials on health and environment is crucial for both modulating their potential toxicity in living organisms and eliminating them from the environment. In this context, understanding if 2D materials are bio-persistent is mandatory. In this review we describe the importance of biodegradability and decomposition of 2D materials. We initially cover the biodegradation of graphene family materials, followed by other emerging classes of 2D materials including transition metal dichalcogenides and oxides, Xenes, Mxenes and other non-metallic 2D materials. We explain the role of defects and functional groups, introduced onto the surface of the materials during their preparation, and the consequences of chemical functionalization on biodegradability. In strong relation to the chemistry on 2D materials, we describe the concept of “degradation-by-design” that we contributed to develop, and which concerns the covalent modification with appropriate molecules to enhance the biodegradability of 2D materials. Finally, we cover the importance of designing new biodegradable 2D conjugates and devices for biomedical applications as drug delivery carriers, in bioelectronics, and tissue engineering. We would like to highlight that the biodegradation of 2D materials mainly depends on the type of material, the chemical functionalization, the aqueous dispersibility and the redox potentials of the different oxidative environments. Biodegradation is one of the necessary conditions for the safe application of 2D materials. Therefore, we hope that this review will help to better understand their biodegradation processes, and will stimulate the chemists to explore new chemical strategies to design safer products, composites and devices containing 2D materials.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
A large number of graphene and other 2D materials are currently explored for the development of new technologies. The assessment of their biodegradability is one of the fundamental aspects for their safe application. , A large number of graphene and other 2D materials are currently used for the development of new technologies, increasingly entering different industrial sectors. Interrogating the impact of such 2D materials on health and environment is crucial for both modulating their potential toxicity in living organisms and eliminating them from the environment. In this context, understanding if 2D materials are bio-persistent is mandatory. In this review we describe the importance of biodegradability and decomposition of 2D materials. We initially cover the biodegradation of graphene family materials, followed by other emerging classes of 2D materials including transition metal dichalcogenides and oxides, Xenes, Mxenes and other non-metallic 2D materials. We explain the role of defects and functional groups, introduced onto the surface of the materials during their preparation, and the consequences of chemical functionalization on biodegradability. In strong relation to the chemistry on 2D materials, we describe the concept of “degradation-by-design” that we contributed to develop, and which concerns the covalent modification with appropriate molecules to enhance the biodegradability of 2D materials. Finally, we cover the importance of designing new biodegradable 2D conjugates and devices for biomedical applications as drug delivery carriers, in bioelectronics, and tissue engineering. We would like to highlight that the biodegradation of 2D materials mainly depends on the type of material, the chemical functionalization, the aqueous dispersibility and the redox potentials of the different oxidative environments. Biodegradation is one of the necessary conditions for the safe application of 2D materials. Therefore, we hope that this review will help to better understand their biodegradation processes, and will stimulate the chemists to explore new chemical strategies to design safer products, composites and devices containing 2D materials.
Luan Xiangfeng, Martín Cristina, Zhang Pengfei, Li Qian, Vacchi Isabella Anna, Delogu Lucia Gemma, Mai Yiyong, Bianco Alberto
Degradation of Structurally Defined Graphene Nanoribbons by Myeloperoxidase and the Photo‐Fenton Reaction Article de journal
Dans: Angewandte Chemie International Edition, vol. 59, no. 42, p. 18515–18521, 2020, ISSN: 1433-7851, 1521-3773.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{luan_degradation_2020,
title = {Degradation of Structurally Defined Graphene Nanoribbons by Myeloperoxidase and the Photo‐Fenton Reaction},
author = {Xiangfeng Luan and Cristina Martín and Pengfei Zhang and Qian Li and Isabella Anna Vacchi and Lucia Gemma Delogu and Yiyong Mai and Alberto Bianco},
url = {https://onlinelibrary.wiley.com/doi/10.1002/anie.202008925},
doi = {10.1002/anie.202008925},
issn = {1433-7851, 1521-3773},
year = {2020},
date = {2020-01-01},
urldate = {2020-11-20},
journal = {Angewandte Chemie International Edition},
volume = {59},
number = {42},
pages = {18515--18521},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
2019
Gurcan Cansu, Taheri Hadiseh, Bianco Alberto, Delogu Lucia Gemma, Yilmazer Acelya
A closer look at the genotoxicity of graphene based materials Article de journal
Dans: Journal of Physics: Materials, vol. 3, no. 1, p. 014007, 2019, ISSN: 2515-7639.
Résumé | Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{gurcan_closer_2019,
title = {A closer look at the genotoxicity of graphene based materials},
author = {Cansu Gurcan and Hadiseh Taheri and Alberto Bianco and Lucia Gemma Delogu and Acelya Yilmazer},
url = {https://doi.org/10.1088%2F2515-7639%2Fab5844},
doi = {10.1088/2515-7639/ab5844},
issn = {2515-7639},
year = {2019},
date = {2019-12-01},
urldate = {2020-04-01},
journal = {Journal of Physics: Materials},
volume = {3},
number = {1},
pages = {014007},
abstract = {Graphene-based materials (GBMs) have attracted many scientists because of their optical, thermal, mechanical and electronic properties. Their good dispersibility in different type of solvents including water, the possibility to formulate them according to desired function, and the wide surface area, which can allow various chemical modifications, expanded the use of these materials in biological systems. For these reasons, GBMs have been extensively studied in vitro and in vivo in the biomedical field. However, the toxicity and genotoxicity of GBMs must be thoroughly investigated before they can be translated into clinical settings. The main mechanism of graphene toxicity is thought to be caused by reactive oxygen species produced in cells, which in turn interact with various biomolecules including DNA. In this review we aimed to discuss different genotoxicity studies performed with GBMs with specific focus on the different cell types and conditions. By comparing and discussing such reports, scientists will be able to engineer non toxic GBMs for future preclinical and/or clinical studies. In order to allow a safer and faster transition to clinic, future studies should involve state-of-the-art technologies such as systems biology approaches or three-dimensional microfluidic systems, which can better predict the normal physiological scenario.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Graphene-based materials (GBMs) have attracted many scientists because of their optical, thermal, mechanical and electronic properties. Their good dispersibility in different type of solvents including water, the possibility to formulate them according to desired function, and the wide surface area, which can allow various chemical modifications, expanded the use of these materials in biological systems. For these reasons, GBMs have been extensively studied in vitro and in vivo in the biomedical field. However, the toxicity and genotoxicity of GBMs must be thoroughly investigated before they can be translated into clinical settings. The main mechanism of graphene toxicity is thought to be caused by reactive oxygen species produced in cells, which in turn interact with various biomolecules including DNA. In this review we aimed to discuss different genotoxicity studies performed with GBMs with specific focus on the different cell types and conditions. By comparing and discussing such reports, scientists will be able to engineer non toxic GBMs for future preclinical and/or clinical studies. In order to allow a safer and faster transition to clinic, future studies should involve state-of-the-art technologies such as systems biology approaches or three-dimensional microfluidic systems, which can better predict the normal physiological scenario.
Bordoni Valentina, Reina Giacomo, Orecchioni Marco, Furesi Giulia, Thiele Stefanie, Gardin Chiara, Zavan Barbara, Cuniberti Gianaurelio, Bianco Alberto, Rauner Martina, Delogu Lucia G
Stimulation of bone formation by monocyte-activator functionalized graphene oxide in vivo Article de journal
Dans: Nanoscale, vol. 11, no. 41, p. 19408–19421, 2019, ISSN: 2040-3372.
Résumé | Liens | BibTeX | Étiquettes: Animals, Biocompatible Materials, Bone Morphogenetic Protein 2, Calcium Phosphates, Cell Differentiation, Cell Survival, Coculture Techniques, Graphite, Humans, I2CT, Inbred C57BL, Male, Mesenchymal Stem Cells, Mice, Monocytes, Oncostatin M, Osteoblasts, Osteogenesis, Signal Transduction, Team-Bianco, Tibia, Wnt Proteins
@article{bordoni_stimulation_2019,
title = {Stimulation of bone formation by monocyte-activator functionalized graphene oxide in vivo},
author = {Valentina Bordoni and Giacomo Reina and Marco Orecchioni and Giulia Furesi and Stefanie Thiele and Chiara Gardin and Barbara Zavan and Gianaurelio Cuniberti and Alberto Bianco and Martina Rauner and Lucia G Delogu},
doi = {10.1039/c9nr03975a},
issn = {2040-3372},
year = {2019},
date = {2019-11-01},
journal = {Nanoscale},
volume = {11},
number = {41},
pages = {19408--19421},
abstract = {Nanosystems are able to enhance bone regeneration, a complex process requiring the mutual interplay between immune and skeletal cells. Activated monocytes can communicate pro-osteogenic signals to mesenchymal stem cells and promote osteogenesis. Thus, the activation of monocytes is a promising strategy to improve bone regeneration. Nanomaterials specifically selected to provoke immune-mediated bone formation are still missing. As a proof of concept, we apply here the intrinsic immune-characteristics of graphene oxide (GO) with the well-recognized osteoinductive capacity of calcium phosphate (CaP) in a biocompatible nanomaterial called maGO-CaP (monocytes activator GO complexed with CaP). In the presence of monocytes, the alkaline phosphatase activity and the expression of osteogenic markers increased. Studying the mechanisms of action, we detected an up-regulation of Wnt and BMP signaling, two key osteogenic pathways. The role of the immune activation was evidenced by the over-production of oncostatin M, a pro-osteogenic factor produced by monocytes. Finally, we tested the pro-osteogenic effects of maGO-CaP in vivo. maGO-CaP injected into the tibia of mice enhanced local bone mass and the bone formation rate. Our study suggests that maGO-CaP can activate monocytes to enhance osteogenesis ex vivo and in vivo.},
keywords = {Animals, Biocompatible Materials, Bone Morphogenetic Protein 2, Calcium Phosphates, Cell Differentiation, Cell Survival, Coculture Techniques, Graphite, Humans, I2CT, Inbred C57BL, Male, Mesenchymal Stem Cells, Mice, Monocytes, Oncostatin M, Osteoblasts, Osteogenesis, Signal Transduction, Team-Bianco, Tibia, Wnt Proteins},
pubstate = {published},
tppubtype = {article}
}
Nanosystems are able to enhance bone regeneration, a complex process requiring the mutual interplay between immune and skeletal cells. Activated monocytes can communicate pro-osteogenic signals to mesenchymal stem cells and promote osteogenesis. Thus, the activation of monocytes is a promising strategy to improve bone regeneration. Nanomaterials specifically selected to provoke immune-mediated bone formation are still missing. As a proof of concept, we apply here the intrinsic immune-characteristics of graphene oxide (GO) with the well-recognized osteoinductive capacity of calcium phosphate (CaP) in a biocompatible nanomaterial called maGO-CaP (monocytes activator GO complexed with CaP). In the presence of monocytes, the alkaline phosphatase activity and the expression of osteogenic markers increased. Studying the mechanisms of action, we detected an up-regulation of Wnt and BMP signaling, two key osteogenic pathways. The role of the immune activation was evidenced by the over-production of oncostatin M, a pro-osteogenic factor produced by monocytes. Finally, we tested the pro-osteogenic effects of maGO-CaP in vivo. maGO-CaP injected into the tibia of mice enhanced local bone mass and the bone formation rate. Our study suggests that maGO-CaP can activate monocytes to enhance osteogenesis ex vivo and in vivo.
Fillatre Jonathan, Fauny Jean-Daniel, Fels Jasmine Alexandra, Li Cheng, Goll Mary, Thisse Christine, Thisse Bernard
TEADs, Yap, Taz, Vgll4s transcription factors control the establishment of Left-Right asymmetry in zebrafish Article de journal
Dans: eLife, vol. 8, 2019, ISSN: 2050-084X.
Résumé | Liens | BibTeX | Étiquettes: Animals, Body Patterning, Developmental, developmental biology, Gene Expression Regulation, Hippo pathway, I2CT, Imagerie, Left-Right asymmetry, Left-Right Organizer, Signal Transduction, Taz, Transcription Factors, Vgll4, Yap, Zebrafish
@article{fillatre_teads_2019,
title = {TEADs, Yap, Taz, Vgll4s transcription factors control the establishment of Left-Right asymmetry in zebrafish},
author = {Jonathan Fillatre and Jean-Daniel Fauny and Jasmine Alexandra Fels and Cheng Li and Mary Goll and Christine Thisse and Bernard Thisse},
doi = {10.7554/eLife.45241},
issn = {2050-084X},
year = {2019},
date = {2019-01-01},
journal = {eLife},
volume = {8},
abstract = {In many vertebrates, establishment of Left-Right (LR) asymmetry results from the activity of a ciliated organ functioning as the LR Organizer (LRO). While regulation of the formation of this structure by major signaling pathways has been described, the transcriptional control of LRO formation is poorly understood. Using the zebrafish model, we show that the transcription factors and cofactors mediating or regulating the transcriptional outcome of the Hippo signaling pathway play a pivotal role in controlling the expression of genes essential to the formation of the LRO including ligands and receptors of signaling pathways involved in this process and most genes required for motile ciliogenesis. Moreover, the transcription cofactor, Vgll4l regulates epigenetic programming in LRO progenitors by controlling the expression of writers and readers of DNA methylation marks. Altogether, our study uncovers a novel and essential role for the transcriptional effectors and regulators of the Hippo pathway in establishing LR asymmetry.},
keywords = {Animals, Body Patterning, Developmental, developmental biology, Gene Expression Regulation, Hippo pathway, I2CT, Imagerie, Left-Right asymmetry, Left-Right Organizer, Signal Transduction, Taz, Transcription Factors, Vgll4, Yap, Zebrafish},
pubstate = {published},
tppubtype = {article}
}
In many vertebrates, establishment of Left-Right (LR) asymmetry results from the activity of a ciliated organ functioning as the LR Organizer (LRO). While regulation of the formation of this structure by major signaling pathways has been described, the transcriptional control of LRO formation is poorly understood. Using the zebrafish model, we show that the transcription factors and cofactors mediating or regulating the transcriptional outcome of the Hippo signaling pathway play a pivotal role in controlling the expression of genes essential to the formation of the LRO including ligands and receptors of signaling pathways involved in this process and most genes required for motile ciliogenesis. Moreover, the transcription cofactor, Vgll4l regulates epigenetic programming in LRO progenitors by controlling the expression of writers and readers of DNA methylation marks. Altogether, our study uncovers a novel and essential role for the transcriptional effectors and regulators of the Hippo pathway in establishing LR asymmetry.
Murera Diane, Malaganahalli Sowmya, Martín Cristina, Reina Giacomo, Fauny Jean-Daniel, Dumortier Hélène, Vázquez Ester, Bianco Alberto
Few layer graphene does not affect the function and the autophagic activity of primary lymphocytes Article de journal
Dans: Nanoscale, vol. 11, no. 21, p. 10493–10503, 2019, ISSN: 2040-3372.
Résumé | Liens | BibTeX | Étiquettes: Animals, Autophagy, B-Lymphocytes, Dumortier, Graphite, I2CT, Inbred BALB C, Mice, Nanostructures, T-Lymphocytes, Team-Bianco, Team-Dumortier
@article{murera_few_2019,
title = {Few layer graphene does not affect the function and the autophagic activity of primary lymphocytes},
author = {Diane Murera and Sowmya Malaganahalli and Cristina Martín and Giacomo Reina and Jean-Daniel Fauny and Hélène Dumortier and Ester Vázquez and Alberto Bianco},
doi = {10.1039/c9nr00846b},
issn = {2040-3372},
year = {2019},
date = {2019-01-01},
journal = {Nanoscale},
volume = {11},
number = {21},
pages = {10493--10503},
abstract = {Carbon-based nanomaterials represent a new tool in future medical applications. Thus, focusing on the evaluation of the degree of their safety has been growing in the last years. In this study we were particularly interested in understanding the impact of few layer graphene (FLG) on primary murine lymphocytes. These B and T cells, that are the second, but specialized, line of defense of the immune system, rely on various mechanisms to ensure their efficient function and maintenance. One of these mechanisms is autophagy that can be triggered by various nanomaterials in some types of cells. For these reasons, we were interested in evaluating the way FLG could affect this process in lymphocytes. Our results point out that FLG neither impacts the viability and activation of T and B cells nor their autophagic activity. Using confocal microscopy, we were also able to see that FLG does not appear to cause any membrane damage and does not penetrate inside of these cells. Overall, our data do not show any effect of this material on lymphocyte homeostasis, which is one more argument in favor of the continuation of studies investigating the potential of FLG for therapeutic applications.},
keywords = {Animals, Autophagy, B-Lymphocytes, Dumortier, Graphite, I2CT, Inbred BALB C, Mice, Nanostructures, T-Lymphocytes, Team-Bianco, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}
Carbon-based nanomaterials represent a new tool in future medical applications. Thus, focusing on the evaluation of the degree of their safety has been growing in the last years. In this study we were particularly interested in understanding the impact of few layer graphene (FLG) on primary murine lymphocytes. These B and T cells, that are the second, but specialized, line of defense of the immune system, rely on various mechanisms to ensure their efficient function and maintenance. One of these mechanisms is autophagy that can be triggered by various nanomaterials in some types of cells. For these reasons, we were interested in evaluating the way FLG could affect this process in lymphocytes. Our results point out that FLG neither impacts the viability and activation of T and B cells nor their autophagic activity. Using confocal microscopy, we were also able to see that FLG does not appear to cause any membrane damage and does not penetrate inside of these cells. Overall, our data do not show any effect of this material on lymphocyte homeostasis, which is one more argument in favor of the continuation of studies investigating the potential of FLG for therapeutic applications.
Reina Giacomo, Zhao Li, Bianco Alberto, Komatsu Naoki
Chemical Functionalization of Nanodiamonds: Opportunities and Challenges Ahead Article de journal
Dans: Angewandte Chemie International Edition, vol. 58, no. 50, p. 17918–17929, 2019, ISSN: 1521-3773.
Résumé | Liens | BibTeX | Étiquettes: carbon materials, diamond, Functionalization, I2CT, imaging, Team-Bianco, therapy
@article{reina_chemical_2019,
title = {Chemical Functionalization of Nanodiamonds: Opportunities and Challenges Ahead},
author = {Giacomo Reina and Li Zhao and Alberto Bianco and Naoki Komatsu},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201905997},
doi = {10.1002/anie.201905997},
issn = {1521-3773},
year = {2019},
date = {2019-01-01},
urldate = {2020-04-01},
journal = {Angewandte Chemie International Edition},
volume = {58},
number = {50},
pages = {17918--17929},
abstract = {Nanodiamond(ND)-based technologies are flourishing in a wide variety of fields spanning from electronics and optics to biomedicine. NDs are considered a family of nanomaterials with an sp3 carbon core and a variety of sizes, shapes, and surfaces. They show interesting physicochemical properties such as hardness, stiffness, and chemical stability. Additionally, they can undergo ad-hoc core and surface functionalization, which tailors them for the desired applications. Noteworthy, the properties of NDs and their surface chemistry are highly dependent on the synthetic method used to prepare them. In this Minireview, we describe the preparation of NDs from the materials-chemistry viewpoint. The different methodologies of synthesis, purification, and surface functionalization as well as biomedical applications are critically discussed. New synthetic approaches as well as limits and obstacles of NDs are presented and analyzed.},
keywords = {carbon materials, diamond, Functionalization, I2CT, imaging, Team-Bianco, therapy},
pubstate = {published},
tppubtype = {article}
}
Nanodiamond(ND)-based technologies are flourishing in a wide variety of fields spanning from electronics and optics to biomedicine. NDs are considered a family of nanomaterials with an sp3 carbon core and a variety of sizes, shapes, and surfaces. They show interesting physicochemical properties such as hardness, stiffness, and chemical stability. Additionally, they can undergo ad-hoc core and surface functionalization, which tailors them for the desired applications. Noteworthy, the properties of NDs and their surface chemistry are highly dependent on the synthetic method used to prepare them. In this Minireview, we describe the preparation of NDs from the materials-chemistry viewpoint. The different methodologies of synthesis, purification, and surface functionalization as well as biomedical applications are critically discussed. New synthetic approaches as well as limits and obstacles of NDs are presented and analyzed.
Rive Corvin, Reina Giacomo, Wagle Prerana, Treossi Emanuele, Palermo Vincenzo, Bianco Alberto, Delogu Lucia Gemma, Rieckher Matthias, Schumacher Björn
Improved Biocompatibility of Amino-Functionalized Graphene Oxide in Caenorhabditis elegans Article de journal
Dans: Small (Weinheim an Der Bergstrasse, Germany), vol. 15, no. 45, p. e1902699, 2019, ISSN: 1613-6829.
Résumé | Liens | BibTeX | Étiquettes: amino-functionalized graphene oxide, Caenorhabditis elegans, graphene oxide, I2CT, innate immunity, Team-Bianco
@article{rive_improved_2019,
title = {Improved Biocompatibility of Amino-Functionalized Graphene Oxide in Caenorhabditis elegans},
author = {Corvin Rive and Giacomo Reina and Prerana Wagle and Emanuele Treossi and Vincenzo Palermo and Alberto Bianco and Lucia Gemma Delogu and Matthias Rieckher and Björn Schumacher},
doi = {10.1002/smll.201902699},
issn = {1613-6829},
year = {2019},
date = {2019-01-01},
journal = {Small (Weinheim an Der Bergstrasse, Germany)},
volume = {15},
number = {45},
pages = {e1902699},
abstract = {Graphene oxide (GO) holds high promise for diagnostic and therapeutic applications in nanomedicine but reportedly displays immunotoxicity, underlining the need for developing functionalized GO with improved biocompatibility. This study describes adverse effects of GO and amino-functionalized GO (GONH2 ) during Caenorhabditis elegans development and ageing upon acute or chronic exposure. Chronic GO treatment throughout the C. elegans development causes decreased fecundity and a reduction of animal size, while acute treatment does not lead to any measurable physiological decline. However, RNA-Sequencing data reveal that acute GO exposure induces innate immune gene expression. The p38 MAP kinase, PMK-1, which is a well-established master regulator of innate immunity, protects C. elegans from chronic GO toxicity, as pmk-1 mutants show reduced tissue-functionality and facultative vivipary. In a direct comparison, GONH2 exposure does not cause detrimental effects in the wild type or in pmk-1 mutants, and the innate immune response is considerably less pronounced. This work establishes enhanced biocompatibility of amino-functionalized GO in a whole-organism, emphasizing its potential as a biomedical nanomaterial.},
keywords = {amino-functionalized graphene oxide, Caenorhabditis elegans, graphene oxide, I2CT, innate immunity, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Graphene oxide (GO) holds high promise for diagnostic and therapeutic applications in nanomedicine but reportedly displays immunotoxicity, underlining the need for developing functionalized GO with improved biocompatibility. This study describes adverse effects of GO and amino-functionalized GO (GONH2 ) during Caenorhabditis elegans development and ageing upon acute or chronic exposure. Chronic GO treatment throughout the C. elegans development causes decreased fecundity and a reduction of animal size, while acute treatment does not lead to any measurable physiological decline. However, RNA-Sequencing data reveal that acute GO exposure induces innate immune gene expression. The p38 MAP kinase, PMK-1, which is a well-established master regulator of innate immunity, protects C. elegans from chronic GO toxicity, as pmk-1 mutants show reduced tissue-functionality and facultative vivipary. In a direct comparison, GONH2 exposure does not cause detrimental effects in the wild type or in pmk-1 mutants, and the innate immune response is considerably less pronounced. This work establishes enhanced biocompatibility of amino-functionalized GO in a whole-organism, emphasizing its potential as a biomedical nanomaterial.
Lucherelli Matteo Andrea, Raya Jésus, Edelthalhammer Konstantin F, Hauke Frank, Hirsch Andreas, Abellán Gonzalo, Bianco Alberto
A Straightforward Approach to Multifunctional Graphene Article de journal
Dans: Chemistry (Weinheim an Der Bergstrasse, Germany), vol. 25, no. 57, p. 13218–13223, 2019, ISSN: 1521-3765.
Résumé | Liens | BibTeX | Étiquettes: carbon materials, diazonium salts, Functionalization, Graphite, I2CT, orthogonal protection, Team-Bianco
@article{lucherelli_straightforward_2019,
title = {A Straightforward Approach to Multifunctional Graphene},
author = {Matteo Andrea Lucherelli and Jésus Raya and Konstantin F Edelthalhammer and Frank Hauke and Andreas Hirsch and Gonzalo Abellán and Alberto Bianco},
doi = {10.1002/chem.201903165},
issn = {1521-3765},
year = {2019},
date = {2019-01-01},
journal = {Chemistry (Weinheim an Der Bergstrasse, Germany)},
volume = {25},
number = {57},
pages = {13218--13223},
abstract = {Graphene has been covalently functionalized through a one-pot reductive pathway using graphite intercalation compounds (GICs), in particular KC8 , with three different orthogonally protected derivatives of 4-aminobenzylamine. This novel multifunctional platform exhibits excellent bulk functionalization homogeneity (Hbulk ) and degree of addition while preserving the chemical functionalities of the organic addends through different protecting groups, namely: tert-butyloxycarbonyl (Boc), benzyloxycarbonyl (Cbz) and phthalimide (Pht). We have employed (temperature-dependent) statistical Raman spectroscopy (SRS), X-ray photoelectron spectroscopy (XPS), magic angle spinning solid state 13 C NMR (MAS-NMR), and a characterization tool consisting of thermogravimetric analysis coupled with gas chromatography and mass spectrometry (TG-GC-MS) to unambiguously demonstrate the covalent binding and the chemical nature of the different molecular linkers. This work paves the way for the development of smart graphene-based materials of great interest in biomedicine or electronics, to name a few, and will serve as a guide in the design of new 2D multifunctional materials.},
keywords = {carbon materials, diazonium salts, Functionalization, Graphite, I2CT, orthogonal protection, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Graphene has been covalently functionalized through a one-pot reductive pathway using graphite intercalation compounds (GICs), in particular KC8 , with three different orthogonally protected derivatives of 4-aminobenzylamine. This novel multifunctional platform exhibits excellent bulk functionalization homogeneity (Hbulk ) and degree of addition while preserving the chemical functionalities of the organic addends through different protecting groups, namely: tert-butyloxycarbonyl (Boc), benzyloxycarbonyl (Cbz) and phthalimide (Pht). We have employed (temperature-dependent) statistical Raman spectroscopy (SRS), X-ray photoelectron spectroscopy (XPS), magic angle spinning solid state 13 C NMR (MAS-NMR), and a characterization tool consisting of thermogravimetric analysis coupled with gas chromatography and mass spectrometry (TG-GC-MS) to unambiguously demonstrate the covalent binding and the chemical nature of the different molecular linkers. This work paves the way for the development of smart graphene-based materials of great interest in biomedicine or electronics, to name a few, and will serve as a guide in the design of new 2D multifunctional materials.
Rauti Rossana, Medelin Manuela, Newman Leon, Vranic Sandra, Reina Giacomo, Bianco Alberto, Prato Maurizio, Kostarelos Kostas, Ballerini Laura
Graphene Oxide Flakes Tune Excitatory Neurotransmission in Vivo by Targeting Hippocampal Synapses Article de journal
Dans: Nano Letters, vol. 19, no. 5, p. 2858–2870, 2019, ISSN: 1530-6992.
Résumé | Liens | BibTeX | Étiquettes: Animals, Excitatory Amino Acid Agents, glutamate, Glutamic Acid, graphene, Graphite, hippocampal network, Hippocampus, Humans, I2CT, Nanostructures, Neurodegenerative Diseases, Neurons, Newborn, Primary Cell Culture, quantum dots, Rats, synapses, Synaptic Transmission, Team-Bianco, Wistar
@article{rauti_graphene_2019,
title = {Graphene Oxide Flakes Tune Excitatory Neurotransmission in Vivo by Targeting Hippocampal Synapses},
author = {Rossana Rauti and Manuela Medelin and Leon Newman and Sandra Vranic and Giacomo Reina and Alberto Bianco and Maurizio Prato and Kostas Kostarelos and Laura Ballerini},
doi = {10.1021/acs.nanolett.8b04903},
issn = {1530-6992},
year = {2019},
date = {2019-01-01},
journal = {Nano Letters},
volume = {19},
number = {5},
pages = {2858--2870},
abstract = {Synapses compute and transmit information to connect neural circuits and are at the basis of brain operations. Alterations in their function contribute to a vast range of neuropsychiatric and neurodegenerative disorders and synapse-based therapeutic intervention, such as selective inhibition of synaptic transmission, may significantly help against serious pathologies. Graphene is a two-dimensional nanomaterial largely exploited in multiple domains of science and technology, including biomedical applications. In hippocampal neurons in culture, small graphene oxide nanosheets (s-GO) selectively depress glutamatergic activity without altering cell viability. Glutamate is the main excitatory neurotransmitter in the central nervous system and growing evidence suggests its involvement in neuropsychiatric disorders. Here we demonstrate that s-GO directly targets the release of presynaptic vesicle. We propose that s-GO flakes reduce the availability of transmitter, via promoting its fast release and subsequent depletion, leading to a decline ofglutamatergic neurotransmission. We injected s-GO in the hippocampus in vivo, and 48 h after surgery ex vivo patch-clamp recordings from brain slices show a significant reduction in glutamatergic synaptic activity in respect to saline injections.},
keywords = {Animals, Excitatory Amino Acid Agents, glutamate, Glutamic Acid, graphene, Graphite, hippocampal network, Hippocampus, Humans, I2CT, Nanostructures, Neurodegenerative Diseases, Neurons, Newborn, Primary Cell Culture, quantum dots, Rats, synapses, Synaptic Transmission, Team-Bianco, Wistar},
pubstate = {published},
tppubtype = {article}
}
Synapses compute and transmit information to connect neural circuits and are at the basis of brain operations. Alterations in their function contribute to a vast range of neuropsychiatric and neurodegenerative disorders and synapse-based therapeutic intervention, such as selective inhibition of synaptic transmission, may significantly help against serious pathologies. Graphene is a two-dimensional nanomaterial largely exploited in multiple domains of science and technology, including biomedical applications. In hippocampal neurons in culture, small graphene oxide nanosheets (s-GO) selectively depress glutamatergic activity without altering cell viability. Glutamate is the main excitatory neurotransmitter in the central nervous system and growing evidence suggests its involvement in neuropsychiatric disorders. Here we demonstrate that s-GO directly targets the release of presynaptic vesicle. We propose that s-GO flakes reduce the availability of transmitter, via promoting its fast release and subsequent depletion, leading to a decline ofglutamatergic neurotransmission. We injected s-GO in the hippocampus in vivo, and 48 h after surgery ex vivo patch-clamp recordings from brain slices show a significant reduction in glutamatergic synaptic activity in respect to saline injections.
Ji Ding-Kun, Ménard-Moyon Cécilia, Bianco Alberto
Physically-triggered nanosystems based on two-dimensional materials for cancer theranostics Article de journal
Dans: Advanced Drug Delivery Reviews, vol. 138, p. 211–232, 2019, ISSN: 1872-8294.
Résumé | Liens | BibTeX | Étiquettes: 2D Materials, Animals, Diagnosis, graphene, Graphite, Humans, I2CT, Light, Magnetic Fields, Nanomaterials, Nanostructures, Neoplasms, Team-Bianco, Theragnosis, Theranostic Nanomedicine, therapy
@article{ji_physically-triggered_2019,
title = {Physically-triggered nanosystems based on two-dimensional materials for cancer theranostics},
author = {Ding-Kun Ji and Cécilia Ménard-Moyon and Alberto Bianco},
doi = {10.1016/j.addr.2018.08.010},
issn = {1872-8294},
year = {2019},
date = {2019-01-01},
journal = {Advanced Drug Delivery Reviews},
volume = {138},
pages = {211--232},
abstract = {There is an increasing demand to develop effective methods for treating malignant diseases to improve healthcare in our society. Stimuli-responsive nanosystems, which can respond to internal or external stimuli are promising in cancer therapy and diagnosis due to their functionality and versatility. As a newly emerging class of nanomaterials, two-dimensional (2D) nanomaterials have attracted huge interest in many different fields including biomedicine due to their unique physical and chemical properties. In the past decade, stimuli-responsive nanosystems based on 2D nanomaterials have been widely studied, showing promising applications in cancer therapy and diagnosis, including phototherapies, magnetic therapy, drug and gene delivery, and non-invasive imaging. Here, we will focus our attention on the state-of-the-art of physically-triggered nanosystems based on graphene and two-dimensional nanomaterials for cancer therapy and diagnosis. The physical triggers include light, temperature, magnetic and electric fields.},
keywords = {2D Materials, Animals, Diagnosis, graphene, Graphite, Humans, I2CT, Light, Magnetic Fields, Nanomaterials, Nanostructures, Neoplasms, Team-Bianco, Theragnosis, Theranostic Nanomedicine, therapy},
pubstate = {published},
tppubtype = {article}
}
There is an increasing demand to develop effective methods for treating malignant diseases to improve healthcare in our society. Stimuli-responsive nanosystems, which can respond to internal or external stimuli are promising in cancer therapy and diagnosis due to their functionality and versatility. As a newly emerging class of nanomaterials, two-dimensional (2D) nanomaterials have attracted huge interest in many different fields including biomedicine due to their unique physical and chemical properties. In the past decade, stimuli-responsive nanosystems based on 2D nanomaterials have been widely studied, showing promising applications in cancer therapy and diagnosis, including phototherapies, magnetic therapy, drug and gene delivery, and non-invasive imaging. Here, we will focus our attention on the state-of-the-art of physically-triggered nanosystems based on graphene and two-dimensional nanomaterials for cancer therapy and diagnosis. The physical triggers include light, temperature, magnetic and electric fields.
2018
Bianco Alberto, Chen Yongsheng, Chen Yuan, Ghoshal Debjit, Hurt Robert H, Kim Yoong Ahm, Koratkar Nikhil, Meunier Vincent, Terrones Mauricio
A carbon science perspective in 2018: Current achievements and future challenges Article de journal
Dans: Carbon, vol. 132, p. 785–801, 2018, ISSN: 0008-6223.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{bianco_carbon_2018,
title = {A carbon science perspective in 2018: Current achievements and future challenges},
author = {Alberto Bianco and Yongsheng Chen and Yuan Chen and Debjit Ghoshal and Robert H Hurt and Yoong Ahm Kim and Nikhil Koratkar and Vincent Meunier and Mauricio Terrones},
url = {http://www.sciencedirect.com/science/article/pii/S0008622318301829},
doi = {10.1016/j.carbon.2018.02.058},
issn = {0008-6223},
year = {2018},
date = {2018-06-01},
urldate = {2020-03-31},
journal = {Carbon},
volume = {132},
pages = {785--801},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Vacchi Isabella A, Raya Jésus, Bianco Alberto, Ménard-Moyon Cécilia
Controlled derivatization of hydroxyl groups of graphene oxide in mild conditions Article de journal
Dans: 2D Materials, vol. 5, no. 3, p. 035037, 2018, ISSN: 2053-1583.
Résumé | Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{vacchi_controlled_2018,
title = {Controlled derivatization of hydroxyl groups of graphene oxide in mild conditions},
author = {Isabella A Vacchi and Jésus Raya and Alberto Bianco and Cécilia Ménard-Moyon},
url = {https://doi.org/10.1088%2F2053-1583%2Faac8a9},
doi = {10.1088/2053-1583/aac8a9},
issn = {2053-1583},
year = {2018},
date = {2018-06-01},
urldate = {2020-04-01},
journal = {2D Materials},
volume = {5},
number = {3},
pages = {035037},
abstract = {Graphene oxide (GO) is constituted of various oxygen-containing functionalities, primarily epoxides and hydroxyl groups on the basal plane, with a very low amount of carbonyl, quinone, carboxylic acid, phenol, and lactone functions at the edges. The high chemical reactivity of these oxygenated groups makes functionalization difficult to control as different reactions can occur concomitantly. In this study we have investigated the reactivity of GO towards orthogonal reactions to selectively functionalize the hydroxyl groups, which are present in a high amount. We explored both the esterification and the Williamson reaction. Our strategies present the main advantage to occur in mild conditions, thus preserving the intrinsic properties of GO, whereas most reactions reported in literature require relatively harsh conditions, leading to (partial) reduction, and/or are not chemoselective. We have also extended our study to the ketones and examined their derivatization by the Wittig reaction. This work has allowed developing two facile methods for the covalent derivatization of the hydroxyl groups in mild conditions, while GO was not reactive towards the Wittig reaction, probably due to the low amount of ketones. Overall, this work leads to a better understanding of the reactivity of GO for controlled derivatization. This opens promising perspectives for multi-functionalization of GO in order to design graphene-based nanomaterials endowed of multiple properties.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Graphene oxide (GO) is constituted of various oxygen-containing functionalities, primarily epoxides and hydroxyl groups on the basal plane, with a very low amount of carbonyl, quinone, carboxylic acid, phenol, and lactone functions at the edges. The high chemical reactivity of these oxygenated groups makes functionalization difficult to control as different reactions can occur concomitantly. In this study we have investigated the reactivity of GO towards orthogonal reactions to selectively functionalize the hydroxyl groups, which are present in a high amount. We explored both the esterification and the Williamson reaction. Our strategies present the main advantage to occur in mild conditions, thus preserving the intrinsic properties of GO, whereas most reactions reported in literature require relatively harsh conditions, leading to (partial) reduction, and/or are not chemoselective. We have also extended our study to the ketones and examined their derivatization by the Wittig reaction. This work has allowed developing two facile methods for the covalent derivatization of the hydroxyl groups in mild conditions, while GO was not reactive towards the Wittig reaction, probably due to the low amount of ketones. Overall, this work leads to a better understanding of the reactivity of GO for controlled derivatization. This opens promising perspectives for multi-functionalization of GO in order to design graphene-based nanomaterials endowed of multiple properties.
Schaeffer Evelyne, Flacher Vincent, Neuberg Patrick, Hoste Astrid, Brulefert Adrien, Fauny Jean-Daniel, Wagner Alain, Mueller Christopher G
Inhibition of dengue virus infection by mannoside glycolipid conjugates Article de journal
Dans: Antiviral Research, vol. 154, p. 116–123, 2018, ISSN: 1872-9096.
Résumé | Liens | BibTeX | Étiquettes: Cell Membrane, Dendritic Cells, Dengue virus, I2CT, Imagerie, inhibitors, Macrophages, Skin, Team-Mueller
@article{schaeffer_inhibition_2018,
title = {Inhibition of dengue virus infection by mannoside glycolipid conjugates},
author = {Evelyne Schaeffer and Vincent Flacher and Patrick Neuberg and Astrid Hoste and Adrien Brulefert and Jean-Daniel Fauny and Alain Wagner and Christopher G Mueller},
doi = {10.1016/j.antiviral.2018.04.005},
issn = {1872-9096},
year = {2018},
date = {2018-01-01},
journal = {Antiviral Research},
volume = {154},
pages = {116--123},
abstract = {Dengue virus (DENV), a mosquito-borne flavivirus, causes severe and potentially fatal symptoms in millions of infected individuals each year. Although dengue fever represents a major global public health problem, the vaccines or antiviral drugs proposed so far have not shown sufficient efficacy and safety, calling for new antiviral developments. Here we have shown that a mannoside glycolipid conjugate (MGC) bearing a trimannose head with a saturated lipid chain inhibited DENV productive infection. It showed remarkable cell promiscuity, being active in human skin dendritic cells, hepatoma cell lines and Vero cells, and was active against all four DENV serotypes, with an IC50 in the low micromolar range. Time-of-addition experiments and structure-activity analyses revealed the importance of the lipid chain to interfere with an early viral infection step. This, together with a correlation between antiviral activity and membrane polarization by the lipid moiety indicated that the inhibitor functions by blocking viral envelope fusion with the endosome membrane. These finding establish MGCs as a novel class of antivirals against the DENV.},
keywords = {Cell Membrane, Dendritic Cells, Dengue virus, I2CT, Imagerie, inhibitors, Macrophages, Skin, Team-Mueller},
pubstate = {published},
tppubtype = {article}
}
Dengue virus (DENV), a mosquito-borne flavivirus, causes severe and potentially fatal symptoms in millions of infected individuals each year. Although dengue fever represents a major global public health problem, the vaccines or antiviral drugs proposed so far have not shown sufficient efficacy and safety, calling for new antiviral developments. Here we have shown that a mannoside glycolipid conjugate (MGC) bearing a trimannose head with a saturated lipid chain inhibited DENV productive infection. It showed remarkable cell promiscuity, being active in human skin dendritic cells, hepatoma cell lines and Vero cells, and was active against all four DENV serotypes, with an IC50 in the low micromolar range. Time-of-addition experiments and structure-activity analyses revealed the importance of the lipid chain to interfere with an early viral infection step. This, together with a correlation between antiviral activity and membrane polarization by the lipid moiety indicated that the inhibitor functions by blocking viral envelope fusion with the endosome membrane. These finding establish MGCs as a novel class of antivirals against the DENV.
Sawaf Matthieu, Fauny Jean-Daniel, Felten Renaud, Sagez Flora, Gottenberg Jacques-Eric, Dumortier Hélène, Monneaux Fanny
Defective BTLA functionality is rescued by restoring lipid metabolism in lupus CD4+ Ŧ cells Article de journal
Dans: JCI insight, vol. 3, no. 13, 2018, ISSN: 2379-3708.
Résumé | Liens | BibTeX | Étiquettes: 80 and over, Adolescent, Adult, Aged, Autoimmune Diseases, Autoimmunity, CD4-Positive T-Lymphocytes, Cell Proliferation, CTLA-4 Antigen, Dumortier, Female, France, Humans, I2CT, Imagerie, Immunologic, Immunology, Lipid Metabolism, lupus, Lupus Erythematosus, Lymphocyte Activation, Male, Middle Aged, Monneaux, Programmed Cell Death 1 Receptor, Receptors, Signal Transduction, Systemic, Team-Dumortier, Young Adult
@article{sawaf_defective_2018,
title = {Defective BTLA functionality is rescued by restoring lipid metabolism in lupus CD4+ Ŧ cells},
author = {Matthieu Sawaf and Jean-Daniel Fauny and Renaud Felten and Flora Sagez and Jacques-Eric Gottenberg and Hélène Dumortier and Fanny Monneaux},
doi = {10.1172/jci.insight.99711},
issn = {2379-3708},
year = {2018},
date = {2018-01-01},
journal = {JCI insight},
volume = {3},
number = {13},
abstract = {Coinhibitory receptors play an important role in the prevention of autoimmune diseases, such as systemic lupus erythematosus (SLE), by limiting T cell activation. B and T lymphocyte attenuator (BTLA) is an inhibitory receptor, similar to cytotoxic T lymphocyte-associated protein 4 (CTLA-4) and programmed death 1 (PD1), that negatively regulates the immune response. The role of BTLA in the pathogenesis of autoimmune diseases in humans and, more specifically, in SLE is largely unknown. We investigated BTLA expression on various T cell subsets, and we did not observe significant variations of BTLA expression between lupus patients and healthy controls. However, the enhancement of BTLA expression after activation was significantly lower in SLE patients compared with that in healthy controls. Furthermore, we found an impaired capacity of BTLA to inhibit T cell activation in SLE due to a poor BTLA recruitment to the immunological synapse following T cell stimulation. Finally, we demonstrated that defective BTLA function can be corrected by restoring intracellular trafficking and by normalizing the lipid metabolism in lupus CD4+ T cells. Collectively, our results evidence that the BTLA signaling pathway is altered in SLE T cells and highlight the potential of targeting this pathway for the development of new therapeutic strategies in lupus.},
keywords = {80 and over, Adolescent, Adult, Aged, Autoimmune Diseases, Autoimmunity, CD4-Positive T-Lymphocytes, Cell Proliferation, CTLA-4 Antigen, Dumortier, Female, France, Humans, I2CT, Imagerie, Immunologic, Immunology, Lipid Metabolism, lupus, Lupus Erythematosus, Lymphocyte Activation, Male, Middle Aged, Monneaux, Programmed Cell Death 1 Receptor, Receptors, Signal Transduction, Systemic, Team-Dumortier, Young Adult},
pubstate = {published},
tppubtype = {article}
}
Coinhibitory receptors play an important role in the prevention of autoimmune diseases, such as systemic lupus erythematosus (SLE), by limiting T cell activation. B and T lymphocyte attenuator (BTLA) is an inhibitory receptor, similar to cytotoxic T lymphocyte-associated protein 4 (CTLA-4) and programmed death 1 (PD1), that negatively regulates the immune response. The role of BTLA in the pathogenesis of autoimmune diseases in humans and, more specifically, in SLE is largely unknown. We investigated BTLA expression on various T cell subsets, and we did not observe significant variations of BTLA expression between lupus patients and healthy controls. However, the enhancement of BTLA expression after activation was significantly lower in SLE patients compared with that in healthy controls. Furthermore, we found an impaired capacity of BTLA to inhibit T cell activation in SLE due to a poor BTLA recruitment to the immunological synapse following T cell stimulation. Finally, we demonstrated that defective BTLA function can be corrected by restoring intracellular trafficking and by normalizing the lipid metabolism in lupus CD4+ T cells. Collectively, our results evidence that the BTLA signaling pathway is altered in SLE T cells and highlight the potential of targeting this pathway for the development of new therapeutic strategies in lupus.
Rodrigues Artur Filipe, Newman Leon, Jasim Dhifaf A, Vacchi Isabella A, Ménard-Moyon Cécilia, Crica Livia E, Bianco Alberto, Kostarelos Kostas, Bussy Cyrill
Immunological impact of graphene oxide sheets in the abdominal cavity is governed by surface reactivity Article de journal
Dans: Archives of Toxicology, vol. 92, no. 11, p. 3359–3379, 2018, ISSN: 1432-0738.
Résumé | Liens | BibTeX | Étiquettes: 2D Materials, Animals, carbon, Epithelium, Female, graphene oxide, Graphite, I2CT, In vivo, Inbred C57BL, inflammation, Intraperitoneal, Macrophages, Mesothelium, Mice, Nanotubes, Peritoneal, Peritoneal Cavity, Protein coating, Team-Bianco, Tissue Distribution, Toxicity
@article{rodrigues_immunological_2018,
title = {Immunological impact of graphene oxide sheets in the abdominal cavity is governed by surface reactivity},
author = {Artur Filipe Rodrigues and Leon Newman and Dhifaf A Jasim and Isabella A Vacchi and Cécilia Ménard-Moyon and Livia E Crica and Alberto Bianco and Kostas Kostarelos and Cyrill Bussy},
doi = {10.1007/s00204-018-2303-z},
issn = {1432-0738},
year = {2018},
date = {2018-01-01},
journal = {Archives of Toxicology},
volume = {92},
number = {11},
pages = {3359--3379},
abstract = {Graphene oxide (GO) is an oxidised form of graphene that has attracted commercial interest in multiple applications, including inks, printed electronics and spray coatings, which all raise health concerns due to potential creation of inhalable aerosols. Although a number of studies have discussed the toxicity of GO sheets, the in vivo impact of their lateral dimensions is still not clear. Here, we compared the effects of large GO sheets (l-GO, 1-20 µm) with those of small GO sheets (s-GO, textbackslashtextless 1 µm) in terms of mesothelial damage and peritoneal inflammation, after intraperitoneal (i.p.) injection in mice. To benchmark the outcomes, long and rigid multi-walled carbon nanotubes (MWCNTs) that were shown to be associated with asbestos-like pathogenicity on the mesothelium were also tested. Our aim was to assess whether lateral dimensions can be a predictor of inflammogenicity for GO sheets in a similar fashion as length is for MWCNTs. While long MWCNTs dispersed in 0.5% BSA induced a granulomatous response on the diaphragmatic mesothelium and immune cell recruitment to the peritoneal cavity, GO sheets dispersed under similar conditions did not cause any response, regardless of their lateral dimensions. We further interrogated whether tuning the surface reactivity of GO by testing different dispersions (5% dextrose instead of 0.5% BSA) may change the biological outcome. Although the change of dispersion did not alter the impact of GO on the mesothelium (i.e. no granuloma), we observed that, when dispersed in protein-free 5% dextrose solution, s-GO elicited a greater recruitment of monocytic cells to the peritoneal cavity than l-GO, or when dispersed in protein-containing solution. Such recruitment coincided with the greater ability of s-GO to interact in vivo with peritoneal macrophages and was associated with a greater surface reactivity in comparison to l-GO. In conclusion, large dimension was not a determining factor of the immunological impact of GO sheets after i.p. administration. For an equal dose, GO sheets with lateral dimensions similar to the length of long MWCNTs were less pathogenic than the MWCNTs. On the other hand, surface reactivity and the ability of some smaller GO sheets to interact more readily with immune cells seem to be key parameters that can be tuned to improve the safety profile of GO. In particular, the choice of dispersion modality, which affected these two parameters, was found to be of crucial importance in the assessment of GO impact in this model. Overall, these findings are essential for a better understanding of the parameters governing GO toxicity and inflammation, and the rational design of safe GO-based formulations for various applications, including biomedicine.},
keywords = {2D Materials, Animals, carbon, Epithelium, Female, graphene oxide, Graphite, I2CT, In vivo, Inbred C57BL, inflammation, Intraperitoneal, Macrophages, Mesothelium, Mice, Nanotubes, Peritoneal, Peritoneal Cavity, Protein coating, Team-Bianco, Tissue Distribution, Toxicity},
pubstate = {published},
tppubtype = {article}
}
Graphene oxide (GO) is an oxidised form of graphene that has attracted commercial interest in multiple applications, including inks, printed electronics and spray coatings, which all raise health concerns due to potential creation of inhalable aerosols. Although a number of studies have discussed the toxicity of GO sheets, the in vivo impact of their lateral dimensions is still not clear. Here, we compared the effects of large GO sheets (l-GO, 1-20 µm) with those of small GO sheets (s-GO, textbackslashtextless 1 µm) in terms of mesothelial damage and peritoneal inflammation, after intraperitoneal (i.p.) injection in mice. To benchmark the outcomes, long and rigid multi-walled carbon nanotubes (MWCNTs) that were shown to be associated with asbestos-like pathogenicity on the mesothelium were also tested. Our aim was to assess whether lateral dimensions can be a predictor of inflammogenicity for GO sheets in a similar fashion as length is for MWCNTs. While long MWCNTs dispersed in 0.5% BSA induced a granulomatous response on the diaphragmatic mesothelium and immune cell recruitment to the peritoneal cavity, GO sheets dispersed under similar conditions did not cause any response, regardless of their lateral dimensions. We further interrogated whether tuning the surface reactivity of GO by testing different dispersions (5% dextrose instead of 0.5% BSA) may change the biological outcome. Although the change of dispersion did not alter the impact of GO on the mesothelium (i.e. no granuloma), we observed that, when dispersed in protein-free 5% dextrose solution, s-GO elicited a greater recruitment of monocytic cells to the peritoneal cavity than l-GO, or when dispersed in protein-containing solution. Such recruitment coincided with the greater ability of s-GO to interact in vivo with peritoneal macrophages and was associated with a greater surface reactivity in comparison to l-GO. In conclusion, large dimension was not a determining factor of the immunological impact of GO sheets after i.p. administration. For an equal dose, GO sheets with lateral dimensions similar to the length of long MWCNTs were less pathogenic than the MWCNTs. On the other hand, surface reactivity and the ability of some smaller GO sheets to interact more readily with immune cells seem to be key parameters that can be tuned to improve the safety profile of GO. In particular, the choice of dispersion modality, which affected these two parameters, was found to be of crucial importance in the assessment of GO impact in this model. Overall, these findings are essential for a better understanding of the parameters governing GO toxicity and inflammation, and the rational design of safe GO-based formulations for various applications, including biomedicine.
Fadeel Bengt, Bussy Cyrill, Merino Sonia, Vázquez Ester, Flahaut Emmanuel, Mouchet Florence, Evariste Lauris, Gauthier Laury, Koivisto Antti J, Vogel Ulla, Martín Cristina, Delogu Lucia G, Buerki-Thurnherr Tina, Wick Peter, Beloin-Saint-Pierre Didier, Hischier Roland, Pelin Marco, Carniel Fabio Candotto, Tretiach Mauro, Cesca Fabrizia, Benfenati Fabio, Scaini Denis, Ballerini Laura, Kostarelos Kostas, Prato Maurizio, Bianco Alberto
Safety Assessment of Graphene-Based Materials: Focus on Human Health and the Environment Article de journal
Dans: ACS nano, vol. 12, no. 11, p. 10582–10620, 2018, ISSN: 1936-086X.
Résumé | Liens | BibTeX | Étiquettes: Animals, carbon nanomaterials, environment, Environmental Monitoring, Exposure, graphene, Graphite, hazard, Health, Humans, I2CT, life cycle assessment, Materials Testing, Nanostructures, Risk Assessment, safety, Structure-Activity Relationship, Team-Bianco, Toxicity
@article{fadeel_safety_2018,
title = {Safety Assessment of Graphene-Based Materials: Focus on Human Health and the Environment},
author = {Bengt Fadeel and Cyrill Bussy and Sonia Merino and Ester Vázquez and Emmanuel Flahaut and Florence Mouchet and Lauris Evariste and Laury Gauthier and Antti J Koivisto and Ulla Vogel and Cristina Martín and Lucia G Delogu and Tina Buerki-Thurnherr and Peter Wick and Didier Beloin-Saint-Pierre and Roland Hischier and Marco Pelin and Fabio Candotto Carniel and Mauro Tretiach and Fabrizia Cesca and Fabio Benfenati and Denis Scaini and Laura Ballerini and Kostas Kostarelos and Maurizio Prato and Alberto Bianco},
doi = {10.1021/acsnano.8b04758},
issn = {1936-086X},
year = {2018},
date = {2018-01-01},
journal = {ACS nano},
volume = {12},
number = {11},
pages = {10582--10620},
abstract = {Graphene and its derivatives are heralded as "miracle" materials with manifold applications in different sectors of society from electronics to energy storage to medicine. The increasing exploitation of graphene-based materials (GBMs) necessitates a comprehensive evaluation of the potential impact of these materials on human health and the environment. Here, we discuss synthesis and characterization of GBMs as well as human and environmental hazard assessment of GBMs using in vitro and in vivo model systems with the aim to understand the properties that underlie the biological effects of these materials; not all GBMs are alike, and it is essential that we disentangle the structure-activity relationships for this class of materials.},
keywords = {Animals, carbon nanomaterials, environment, Environmental Monitoring, Exposure, graphene, Graphite, hazard, Health, Humans, I2CT, life cycle assessment, Materials Testing, Nanostructures, Risk Assessment, safety, Structure-Activity Relationship, Team-Bianco, Toxicity},
pubstate = {published},
tppubtype = {article}
}
Graphene and its derivatives are heralded as "miracle" materials with manifold applications in different sectors of society from electronics to energy storage to medicine. The increasing exploitation of graphene-based materials (GBMs) necessitates a comprehensive evaluation of the potential impact of these materials on human health and the environment. Here, we discuss synthesis and characterization of GBMs as well as human and environmental hazard assessment of GBMs using in vitro and in vivo model systems with the aim to understand the properties that underlie the biological effects of these materials; not all GBMs are alike, and it is essential that we disentangle the structure-activity relationships for this class of materials.
Arosio Paolo, Comito Giuseppina, Orsini Francesco, Lascialfari Alessandro, Chiarugi Paola, Ménard-Moyon Cécilia, Nativi Cristina, Richichi Barbara