Biologie Digitale de l’ARN

@article{,

title = {Molecular insights into the ligand-controlled organization of the SAM-I riboswitch},

author = {B Heppell and S Blouin and A M Dussault and J Mulhbacher and E Ennifar and J C Penedo and D A Lafontaine},

url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=21532599},

doi = {10.1038/nchembio.563},

isbn = {ISBN/1552-4469 (Electronic) 

1552-4450 (Linking)},

year  = {2011},

date = {2011-01-01},

journal = {Nat Chem Biol},

volume = {7},

number = {6},

pages = {384-92},

abstract = {S-adenosylmethionine (SAM) riboswitches are widespread in bacteria, and up to five different SAM riboswitch families have been reported, highlighting the relevance of SAM regulation. On the basis of crystallographic and biochemical data, it has been postulated, but never demonstrated, that ligand recognition by SAM riboswitches involves key conformational changes in the RNA architecture. We show here that the aptamer follows a two-step hierarchical folding selectively induced by metal ions and ligand binding, each of them leading to the formation of one of the two helical stacks observed in the crystal structure. Moreover, we find that the anti-antiterminator P1 stem is rotated along its helical axis upon ligand binding, a mechanistic feature that could be common to other riboswitches. We also show that the nonconserved P4 helical domain is used as an auxiliary element to enhance the ligand-binding affinity. This work provides the first comprehensive characterization, to our knowledge, of a ligand-controlled riboswitch folding pathway.},

note = {Published online 01 May 2011},

keywords = {Aptamers, Bacterial/*chemistry  *Riboswitch  S-Adenosylmethionine/*chemistry, DUMAS, ENNIFAR, Nucleotide/chemistry  Bacillus subtilis/genetics  Binding Sites  Crystallography, Unité ARN, X-Ray  Ligands  Metals  Nucleic Acid Conformation  RNA},

pubstate = {published},

tppubtype = {article}

}

@article{van_den_bossche_efficient_2010,

title = {Efficient receptor-independent intracellular translocation of aptamers mediated by conjugation to carbon nanotubes},

author = {Jeroen Van den Bossche and Wafa' T Al-Jamal and Bowen Tian and Antonio Nunes and Chiara Fabbro and Alberto Bianco and Maurizio Prato and Kostas Kostarelos},

doi = {10.1039/c0cc02092c},

issn = {1364-548X},

year  = {2010},

date = {2010-10-01},

journal = {Chemical Communications (Cambridge, England)},

volume = {46},

number = {39},

pages = {7379--7381},

abstract = {We have covalently grafted aptamers onto carboxylated carbon nanotubes to design a novel vector system that can easily translocate into the cytosol of different cell types independent of receptor-mediated uptake. We propose the use of carbon nanotubes for the efficient intracellular delivery of biologically active aptamers for potential therapeutic applications.},

keywords = {Aptamers, Base Sequence, Biological Transport, carbon, Cell Line, Cell Surface, DNA Primers, Electron, Electrophoresis, Humans, I2CT, Microscopy, Nanotubes, Nucleotide, Polyacrylamide Gel, Receptors, Team-Bianco, Transmission, tumor},

pubstate = {published},

tppubtype = {article}

}