Publications
1997
Eckardt S, Romby P, Sczakiel G
Implications of RNA structure on the annealing of a potent antisense RNA directed against the human immunodeficiency virus type 1 Journal Article
In: Biochemistry, vol. 36, no. 42, pp. 12711-12721, 1997, ISBN: 9335527, (0006-2960 Journal Article).
Abstract | Links | BibTeX | Tags: Anti-HIV Agents/*chemistry/pharmacology Base Sequence Cells, Antisense/*chemistry/*pharmacology RNA, Calf Thymus Ribonuclease T1 Software Support, Cultured Electrophoresis, Genetic, Insertional Mutagenesis, Non-U.S. Gov't Thermodynamics Transcription, Polyacrylamide Gel HIV-1/*drug effects/genetics Human Kinetics Molecular Sequence Data Mutagenesis, ROMBY, Site-Directed *Nucleic Acid Conformation Oligodeoxyribonucleotides Oligoribonucleotides/*chemistry/pharmacology RNA, Unité ARN, Viral/*chemistry/drug effects Ribonuclease H
@article{,
title = {Implications of RNA structure on the annealing of a potent antisense RNA directed against the human immunodeficiency virus type 1},
author = {S Eckardt and P Romby and G Sczakiel},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9335527},
isbn = {9335527},
year = {1997},
date = {1997-01-01},
journal = {Biochemistry},
volume = {36},
number = {42},
pages = {12711-12721},
abstract = {Antisense RNA-mediated regulation in bacterial systems is related to the kinetics of RNA-RNA annealing in vitro. Here, we investigated the secondary structure of alphaY69, an effective HIV-directed antisense RNA in human cells. Purified RNA preparations contain a single conformer. The global structure was identified by a cleavage experiment under native conditions using a short complementary oligonucleotide and RNase H. Structural analyses indicate a three-domain structure of alphaY69 consisting of two stem-loop elements connected by a seven-nucleotide single-stranded hinge region. Kinetic data suggest that the formation of base pairs between a CGC triplet of alphaY69 and its target RNA is essential for fast annealing. The complementary sequence stretch of the target folds into a high-energy secondary structure. The relationship between modifications in structural elements of alphaY69 and the annealing kinetics suggested that rate-limiting steps of the annealing involve a single site of alphaY69 and do not involve its 5' or 3'-end. Further, the data indicate that both initial base-specific interactions and duplex formation are dependent on the CGC triplet of the central region of alphaY69. This mechanism represents a specific and efficient way of RNA-RNA annealing that is initiated by the interaction of unstructured RNA regions.},
note = {0006-2960
Journal Article},
keywords = {Anti-HIV Agents/*chemistry/pharmacology Base Sequence Cells, Antisense/*chemistry/*pharmacology RNA, Calf Thymus Ribonuclease T1 Software Support, Cultured Electrophoresis, Genetic, Insertional Mutagenesis, Non-U.S. Gov't Thermodynamics Transcription, Polyacrylamide Gel HIV-1/*drug effects/genetics Human Kinetics Molecular Sequence Data Mutagenesis, ROMBY, Site-Directed *Nucleic Acid Conformation Oligodeoxyribonucleotides Oligoribonucleotides/*chemistry/pharmacology RNA, Unité ARN, Viral/*chemistry/drug effects Ribonuclease H},
pubstate = {published},
tppubtype = {article}
}