Publications
2018
Muller Quentin, Beaudet Marie-Josée, Serres-Bérard Thiéry De, Bellenfant Sabrina, Flacher Vincent, Berthod François
Development of an innervated tissue-engineered skin with human sensory neurons and Schwann cells differentiated from iPS cells Journal Article
In: Acta Biomaterialia, vol. 82, pp. 93–101, 2018, ISSN: 1878-7568.
Abstract | Links | BibTeX | Tags: atopic dermatitis, Axonal migration, Biological, Canada, Cells, CGRP, Chemistry, COLLAGEN, Culture, Dermatitis, development, disease, Endothelial Cells, ENDOTHELIAL-CELLS, Epidermis, Expression, Fibroblast, Fibroblasts, function, Human, Humans, Immune System, Immunology, immunopathology, IN VITRO, Induced Pluripotent Stem Cells, inflammation, INNERVATION, Maturation, migration, Models, mouse, murine, Nerve, Neurites, Neurogenic Inflammation, Neurons, NEUROPEPTIDE, Neuropeptides, physiopathology, Pluripotent Stem Cells, Psoriasis, SCHWANN CELLS, Sensory Receptor Cells, Skin, skin disease, Skin Diseases, stem, Stem Cells, SUBSTANCE, SUBSTANCE P, Team-Mueller, Tissue Engineering, TRPV1
@article{muller_development_2018,
title = {Development of an innervated tissue-engineered skin with human sensory neurons and Schwann cells differentiated from iPS cells},
author = {Quentin Muller and Marie-Josée Beaudet and Thiéry De Serres-Bérard and Sabrina Bellenfant and Vincent Flacher and François Berthod},
doi = {10.1016/j.actbio.2018.10.011},
issn = {1878-7568},
year = {2018},
date = {2018-01-01},
journal = {Acta Biomaterialia},
volume = {82},
pages = {93--101},
abstract = {Cutaneous innervation is increasingly recognized as a major element of skin physiopathology through the neurogenic inflammation driven by neuropeptides that are sensed by endothelial cells and the immune system. To investigate this process in vitro, models of innervated tissue-engineered skin (TES) were developed, yet exclusively with murine sensory neurons extracted from dorsal root ganglions. In order to build a fully human model of innervated TES, we used induced pluripotent stem cells (iPSC) generated from human skin fibroblasts. Nearly 100% of the iPSC differentiated into sensory neurons were shown to express the neuronal markers BRN3A and β3-tubulin after 19 days of maturation. In addition, these cells were also positive to TRPV1 and neurofilament M, and some of them expressed Substance P, TrkA and TRPA1. When stimulated with molecules inducing neuropeptide release, iPSC-derived neurons released Substance P and CGRP, both in conventional monolayer culture and after seeding in a 3D fibroblast-populated collagen sponge model. Schwann cells, the essential partners of neurons for function and axonal migration, were also successfully differentiated from human iPSC as shown by their expression of the markers S100, GFAP, p75 and SOX10. When cultured for one additional month in the TES model, iPSC-derived neurons seeded at the bottom of the sponge formed a network of neurites spanning the whole TES up to the epidermis, but only when combined with mouse or iPSC-derived Schwann cells. This unique model of human innervated TES should be highly useful for the study of cutaneous neuroinflammation. STATEMENT OF SIGNIFICANCE: The purpose of this work was to develop in vitro an innovative fully human tissue-engineered skin enabling the investigation of the influence of cutaneous innervation on skin pathophysiology. To reach that aim, neurons were differentiated from human induced pluripotent stem cells (iPSCs) generated from normal human skin fibroblasts. This innervated tissue-engineered skin model will be the first one to show iPSC-derived neurons can be successfully used to build a 3D nerve network in vitro. Since innervation has been recently recognized to play a central role in many human skin diseases, such as psoriasis and atopic dermatitis, this construct promises to be at the forefront to model these diseases while using patient-derived cells.},
keywords = {atopic dermatitis, Axonal migration, Biological, Canada, Cells, CGRP, Chemistry, COLLAGEN, Culture, Dermatitis, development, disease, Endothelial Cells, ENDOTHELIAL-CELLS, Epidermis, Expression, Fibroblast, Fibroblasts, function, Human, Humans, Immune System, Immunology, immunopathology, IN VITRO, Induced Pluripotent Stem Cells, inflammation, INNERVATION, Maturation, migration, Models, mouse, murine, Nerve, Neurites, Neurogenic Inflammation, Neurons, NEUROPEPTIDE, Neuropeptides, physiopathology, Pluripotent Stem Cells, Psoriasis, SCHWANN CELLS, Sensory Receptor Cells, Skin, skin disease, Skin Diseases, stem, Stem Cells, SUBSTANCE, SUBSTANCE P, Team-Mueller, Tissue Engineering, TRPV1},
pubstate = {published},
tppubtype = {article}
}
2017
Russier Julie, León Verónica, Orecchioni Marco, Hirata Eri, Virdis Patrizia, Fozza Claudio, Sgarrella Francesco, Cuniberti Gianaurelio, Prato Maurizio, Vázquez Ester, Bianco Alberto, Delogu Lucia G
Few-Layer Graphene Kills Selectively Tumor Cells from Myelomonocytic Leukemia Patients Journal Article
In: Angewandte Chemie (International Ed. in English), vol. 56, no. 11, pp. 3014–3019, 2017, ISSN: 1521-3773.
Abstract | Links | BibTeX | Tags: Acute, cancer therapy, Chronic, Cultured, graphene, Graphite, Humans, I2CT, Immune System, leukemia, Leukocytes, Mononuclear, Myeloid, Myelomonocytic, myelomonocytic leukemia, Nanomaterials, Particle Size, Surface Properties, Team-Bianco, Tumor Cells
@article{russier_few-layer_2017,
title = {Few-Layer Graphene Kills Selectively Tumor Cells from Myelomonocytic Leukemia Patients},
author = {Julie Russier and Verónica León and Marco Orecchioni and Eri Hirata and Patrizia Virdis and Claudio Fozza and Francesco Sgarrella and Gianaurelio Cuniberti and Maurizio Prato and Ester Vázquez and Alberto Bianco and Lucia G Delogu},
doi = {10.1002/anie.201700078},
issn = {1521-3773},
year = {2017},
date = {2017-01-01},
journal = {Angewandte Chemie (International Ed. in English)},
volume = {56},
number = {11},
pages = {3014--3019},
abstract = {In the cure of cancer, a major cause of today's mortality, chemotherapy is the most common treatment, though serious frequent challenges are encountered by current anticancer drugs. We discovered that few-layer graphene (FLG) dispersions have a specific killer action on monocytes, showing neither toxic nor activation effects on other immune cells. We confirmed the therapeutic application of graphene on an aggressive type of cancer that is myelomonocytic leukemia, where the monocytes are in their malignant form. We demonstrated that graphene has the unique ability to target and boost specifically the necrosis of monocytic cancer cells. Moreover, the comparison between FLG and a common chemotherapeutic drug, etoposide, confirmed the higher specificity and toxicity of FLG. Since current chemotherapy treatments of leukemia still cause serious problems, these findings open the way to new and safer therapeutic approaches.},
keywords = {Acute, cancer therapy, Chronic, Cultured, graphene, Graphite, Humans, I2CT, Immune System, leukemia, Leukocytes, Mononuclear, Myeloid, Myelomonocytic, myelomonocytic leukemia, Nanomaterials, Particle Size, Surface Properties, Team-Bianco, Tumor Cells},
pubstate = {published},
tppubtype = {article}
}
2013
Dumortier Hélène
When carbon nanotubes encounter the immune system: desirable and undesirable effects Journal Article
In: Advanced Drug Delivery Reviews, vol. 65, no. 15, pp. 2120–2126, 2013, ISSN: 1872-8294.
Abstract | Links | BibTeX | Tags: Animals, Biomedical application, carbon, Carbon nanotubes, Dumortier, Environmental Exposure, Functionalization, Humans, I2CT, Immune cell activation, Immune System, inflammation, Inhalation Exposure, Lymphocyte, Macrophage, Nanotubes, Occupational Exposure, Team-Dumortier, Toxicity
@article{dumortier_when_2013,
title = {When carbon nanotubes encounter the immune system: desirable and undesirable effects},
author = {Hélène Dumortier},
doi = {10.1016/j.addr.2013.09.005},
issn = {1872-8294},
year = {2013},
date = {2013-01-01},
journal = {Advanced Drug Delivery Reviews},
volume = {65},
number = {15},
pages = {2120--2126},
abstract = {The role of our immune system is to bring efficient protection against invasion by foreign elements, not only pathogens but also any material it may be in contact with. Nanoparticles may enter the body and encounter the immune system either intentionally (e.g. administration for biomedical application) or not (e.g. respiratory occupational exposure). Therefore, it is of fundamental importance to get a thorough knowledge of the way they interact with immune cells and all related consequences. Among nanomaterials, carbon nanotubes (CNTs) are of special interest because of their tremendous field of applications. Consequently, their increasing production, processing and eventual incorporation into new types of composites and/or into biological systems have raised fundamental issues regarding their potential impact on health. This review aims at giving an overview of the known desirable and undesirable effects of CNTs on the immune system, i.e. beneficial modulation of immune cells by CNTs engineered for biomedical applications versus toxicity, inflammation and unwanted immune reactions triggered by CNTs themselves.},
keywords = {Animals, Biomedical application, carbon, Carbon nanotubes, Dumortier, Environmental Exposure, Functionalization, Humans, I2CT, Immune cell activation, Immune System, inflammation, Inhalation Exposure, Lymphocyte, Macrophage, Nanotubes, Occupational Exposure, Team-Dumortier, Toxicity},
pubstate = {published},
tppubtype = {article}
}
2012
Meister Marie, Ferrandon Dominique
Immune cell transdifferentiation: a complex crosstalk between circulating immune cells and the haematopoietic niche Journal Article
In: EMBO Rep., vol. 13, no. 1, pp. 3–4, 2012, ISSN: 1469-3178.
Links | BibTeX | Tags: Animals, Cell Communication, Cell Transdifferentiation, ferrandon, Hematopoietic Stem Cells, Humans, Immune System, M3i, Signal Transduction, Stem Cell Niche
@article{meister_immune_2012,
title = {Immune cell transdifferentiation: a complex crosstalk between circulating immune cells and the haematopoietic niche},
author = {Marie Meister and Dominique Ferrandon},
doi = {10.1038/embor.2011.238},
issn = {1469-3178},
year = {2012},
date = {2012-01-01},
journal = {EMBO Rep.},
volume = {13},
number = {1},
pages = {3--4},
keywords = {Animals, Cell Communication, Cell Transdifferentiation, ferrandon, Hematopoietic Stem Cells, Humans, Immune System, M3i, Signal Transduction, Stem Cell Niche},
pubstate = {published},
tppubtype = {article}
}
Hess E, Duheron V, Decossas M, Lezot F, Berdal A, Chea S, Golub R, Bosisio M R, Bridal S L, Choi Y, Yagita H, Mueller C G
RANKL induces organized lymph node growth by stromal cell proliferation Journal Article
In: Journal of Immunology, vol. 188, no. 1550-6606 (Electronic), pp. 1245–1254, 2012.
Abstract | Links | BibTeX | Tags: Animals, Cell Adhesion, Cell Adhesion Molecules, Cell Proliferation, Chemokine CCL19, Chemokine CXCL13, chemokines, CXCL13, cytology, development, Growth, growth & development, Hair, hair follicle, Homeostasis, Human, Immune System, Immunization, ligand, LYMPH, LYMPH NODE, Lymph Nodes, Mice, mouse, physiology, plasticity, Proliferation, Protein, rank, RANK ligand, Regulation, Secondary, Stromal Cells, Team-Mueller, transgenic, VCAM1
@article{hess_rankl_2012,
title = {RANKL induces organized lymph node growth by stromal cell proliferation},
author = {E Hess and V Duheron and M Decossas and F Lezot and A Berdal and S Chea and R Golub and M R Bosisio and S L Bridal and Y Choi and H Yagita and C G Mueller},
doi = {10.4049/jimmunol.1101513},
year = {2012},
date = {2012-01-01},
journal = {Journal of Immunology},
volume = {188},
number = {1550-6606 (Electronic)},
pages = {1245--1254},
abstract = {RANK and its ligand RANKL play important roles in the development and regulation of the immune system. We show that mice transgenic for Rank in hair follicles display massive postnatal growth of skin-draining lymph nodes. The proportions of hematopoietic and nonhematopoietic stromal cells and their organization are maintained, with the exception of an increase in B cell follicles. The hematopoietic cells are not activated and respond to immunization by foreign Ag and adjuvant. We demonstrate that soluble RANKL is overproduced from the transgenic hair follicles and that its neutralization normalizes lymph node size, inclusive area, and numbers of B cell follicles. Reticular fibroblastic and vascular stromal cells, important for secondary lymphoid organ formation and organization, express RANK and undergo hyperproliferation, which is abrogated by RANKL neutralization. In addition, they express higher levels of CXCL13 and CCL19 chemokines, as well as MAdCAM-1 and VCAM-1 cell-adhesion molecules. These findings highlight the importance of tissue-derived cues for secondary lymphoid organ homeostasis and identify RANKL as a key molecule for controlling the plasticity of the immune system},
keywords = {Animals, Cell Adhesion, Cell Adhesion Molecules, Cell Proliferation, Chemokine CCL19, Chemokine CXCL13, chemokines, CXCL13, cytology, development, Growth, growth & development, Hair, hair follicle, Homeostasis, Human, Immune System, Immunization, ligand, LYMPH, LYMPH NODE, Lymph Nodes, Mice, mouse, physiology, plasticity, Proliferation, Protein, rank, RANK ligand, Regulation, Secondary, Stromal Cells, Team-Mueller, transgenic, VCAM1},
pubstate = {published},
tppubtype = {article}
}
2008
Romani N, Ebner S, Flacher V, Tripp C H, Heufler C, Clausen B E, Stoitzner P
Langerhans cells - dendritic cells of the epidermis and other epithelia Book Section
In: Saeland, S (Ed.): Recent Advances in Skin Immunology, Research Signpost, Trivandrum, Kerala, India, 2008.
Abstract | BibTeX | Tags: BLOOD, Dendritic Cells, Epidermis, Epithelium, function, Immune System, Immunology, In vivo, Langerhans Cells, Skin, Team-Mueller
@incollection{romani_langerhans_2008,
title = {Langerhans cells - dendritic cells of the epidermis and other epithelia},
author = {N Romani and S Ebner and V Flacher and C H Tripp and C Heufler and B E Clausen and P Stoitzner},
editor = {S Saeland},
year = {2008},
date = {2008-01-01},
booktitle = {Recent Advances in Skin Immunology},
publisher = {Research Signpost},
address = {Trivandrum, Kerala, India},
abstract = {Langerhans cells are dendritic cells that reside in epithelia, formeost in the epidermis. Like dendritic cells from non-epithelial tissues or from the blood, they form a functional bridge between the innate and the adaptive immune system. Although Langerhans cells have first been described 140 years ago, only recently has a lively scientific debate arisen as to their functional role in vivo, i.e., in the living organism. This is mainly due to the advent of modern, sophisticated experimental models that allow to tackle hitherto unaddressed problems. It is not yet entirely clear whether an immunogenic or a tolerogenic function of Langerhans cells prevails in vivo. Here, we attempt to summarize and discuss the current knowledge on the immunobiology of Langerhans cells with emphasis on their role in vivo.},
keywords = {BLOOD, Dendritic Cells, Epidermis, Epithelium, function, Immune System, Immunology, In vivo, Langerhans Cells, Skin, Team-Mueller},
pubstate = {published},
tppubtype = {incollection}
}
2007
Ferrandon Dominique, Gottar Marie, Gobert Vanessa
[New mechanism for detection of infections using the innate immune system of animals] Journal Article
In: Med Sci (Paris), vol. 23, no. 8-9, pp. 707–709, 2007, ISSN: 0767-0974.
Links | BibTeX | Tags: Animal, Animals, Drosophila/immunology, ferrandon, Gram-Positive Bacteria, Gram-Positive Bacteria/pathogenicity, Gram-Positive Bacterial Infections, Gram-Positive Bacterial Infections/immunology, Humans, Immune System, infection, Infection/*diagnosis/*immunology, M3i, Models
@article{ferrandon_[new_2007b,
title = {[New mechanism for detection of infections using the innate immune system of animals]},
author = {Dominique Ferrandon and Marie Gottar and Vanessa Gobert},
doi = {10.1051/medsci/20072389707},
issn = {0767-0974},
year = {2007},
date = {2007-09-01},
journal = {Med Sci (Paris)},
volume = {23},
number = {8-9},
pages = {707--709},
keywords = {Animal, Animals, Drosophila/immunology, ferrandon, Gram-Positive Bacteria, Gram-Positive Bacteria/pathogenicity, Gram-Positive Bacterial Infections, Gram-Positive Bacterial Infections/immunology, Humans, Immune System, infection, Infection/*diagnosis/*immunology, M3i, Models},
pubstate = {published},
tppubtype = {article}
}
Monneaux Fanny, Muller Sylviane
Peptide-based therapy in lupus: promising data Journal Article
In: Advances in Experimental Medicine and Biology, vol. 601, pp. 105–112, 2007, ISSN: 0065-2598.
Abstract | Links | BibTeX | Tags: Adrenal Cortex Hormones, Animals, Autoantigens, Autoimmune Diseases, Cyclophosphamide, Epitopes, Humans, I2CT, Immune System, Immunosuppressive Agents, inflammation, Lupus Erythematosus, Monneaux, Peptides, Systemic, T-Lymphocytes, Team-Dumortier
@article{monneaux_peptide-based_2007,
title = {Peptide-based therapy in lupus: promising data},
author = {Fanny Monneaux and Sylviane Muller},
doi = {10.1007/978-0-387-72005-0_11},
issn = {0065-2598},
year = {2007},
date = {2007-01-01},
journal = {Advances in Experimental Medicine and Biology},
volume = {601},
pages = {105--112},
abstract = {Systemic lupus erythematosus (SLE) is a multisystem chronic inflammatory disease of multifactorial aetiology, characterized by inflammation and damage of various tissues and organs. Current treatments of the disease are mainly based on immunosuppressive drugs such as corticosteroids and cyclophosphamide. Although these treatments have reduced mortality and morbidity, they cause a non-specific immune suppression. To avoid these side effects, our efforts should focus on the development of alternative therapeutic strategies, which consist, for example in specific T cell targeting using autoantigen-derived peptides identified as sequences encompassing major epitopes.},
keywords = {Adrenal Cortex Hormones, Animals, Autoantigens, Autoimmune Diseases, Cyclophosphamide, Epitopes, Humans, I2CT, Immune System, Immunosuppressive Agents, inflammation, Lupus Erythematosus, Monneaux, Peptides, Systemic, T-Lymphocytes, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}