Publications
2021
Holleufer Andreas, Winther Kasper Grønbjerg, Gad Hans Henrik, Ai Xianlong, Chen Yuqiang, Li Lihua, Wei Ziming, Deng Huimin, Liu Jiyong, Frederiksen Ninna Ahlmann, Simonsen Bine, Andersen Line Lykke, Kleigrewe Karin, Dalskov Louise, Pichlmair Andreas, Cai Hua, Imler Jean-Luc, Hartmann Rune
Two cGAS-like receptors induce antiviral immunity in Drosophila Article de journal
Dans: Nature, vol. 597, p. 114-118, 2021.
Résumé | Liens | BibTeX | Étiquettes: antiviral immunity, cGAS-like receptors, Drosophila, imler, M3i
@article{Hartmann2021,
title = {Two cGAS-like receptors induce antiviral immunity in Drosophila},
author = {Andreas Holleufer and Kasper Grønbjerg Winther and Hans Henrik Gad and Xianlong Ai and Yuqiang Chen and Lihua Li and Ziming Wei and Huimin Deng and Jiyong Liu and Ninna Ahlmann Frederiksen and Bine Simonsen and Line Lykke Andersen and Karin Kleigrewe and Louise Dalskov and Andreas Pichlmair and Hua Cai and Jean-Luc Imler and Rune Hartmann},
editor = {Nature Publishing Group},
doi = {https://doi.org/10.1038/s41586-021-03800-z},
year = {2021},
date = {2021-07-14},
urldate = {2021-07-14},
journal = {Nature},
volume = {597},
pages = {114-118},
abstract = {In mammals, cyclic GMP-AMP (cGAMP) synthase (cGAS) produces the cyclic dinucleotide (CDN) 2'3'-cGAMP in response to cytosolic DNA and this triggers an antiviral immune response. cGAS belongs to a large family of cGAS/DncV-like nucleotidyltransferases, present in both prokaryotes1 and eukaryotes2–5. In bacteria, these enzymes synthesize a range of cyclic oligonucleotide and have recently emerged as important regulators of phage infections6–8. Here, we identify two novel cGAS-like receptors (cGLRs) in the insect Drosophila melanogaster. We show that cGLR1 and cGLR2 activate Sting and NF-κB dependent antiviral immunity in response to infection with RNA or DNA viruses. cGLR1 is activated by dsRNA to produce the novel CDN 3'2'-cGAMP whereas cGLR2 produces a combination of 2'3'-cGAMP and 3'2' cGAMP in response to a yet unidentified stimulus. Our data establish cGAS as the founding member of a family of receptors sensing different types of nucleic acids and triggering immunity through production of CDNs beyond 2'3'-cGAMP.},
keywords = {antiviral immunity, cGAS-like receptors, Drosophila, imler, M3i},
pubstate = {published},
tppubtype = {article}
}
2019
Olmo RP, Martins NE, Aguiar ERGR, Marques JT, Imler JL
The insect reservoir of biodiversity for viruses and for antiviral mechanisms Article de journal
Dans: An Acad Bras Cienc , vol. 91, no. Suppl 3, p. e20190122, 2019.
Résumé | Liens | BibTeX | Étiquettes: antiviral immunity, imler, insects, M3i, Marques, metagenomics, restriction factors, RNA Interference, virome
@article{Olmo_2019,
title = {The insect reservoir of biodiversity for viruses and for antiviral mechanisms},
author = {RP Olmo and NE Martins and ERGR Aguiar and JT Marques and JL Imler},
url = {https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000600604&lng=en&nrm=iso&tlng=en},
doi = {10.1590/0001-3765201920190122},
year = {2019},
date = {2019-06-03},
journal = { An Acad Bras Cienc },
volume = {91},
number = {Suppl 3},
pages = {e20190122},
abstract = {Insects are the most diverse group of animals. They can be infected by an extraordinary diversity of viruses. Among them, arthropod-borne viruses (arboviruses) can be transmitted to humans. High-throughput sequencing of small RNAs from insects provides insight into their virome, which may help understand the dynamics of vector borne infectious diseases. Furthermore, investigating the mechanisms that restrict viral infections in insects points to genetic innovations that may inspire novel antiviral strategies. },
keywords = {antiviral immunity, imler, insects, M3i, Marques, metagenomics, restriction factors, RNA Interference, virome},
pubstate = {published},
tppubtype = {article}
}
Martins NE, Olmo RP, Aguiar ERGR, Marques JT, Imler JL
Les insectes : un fantastique réservoir de virus et de gènes antiviraux Article de journal
Dans: Biologie Aujourd'hui, vol. 212, no. 3-4, p. 101-106, 2019.
Résumé | Liens | BibTeX | Étiquettes: antiviral immunity, ARN interference, imler, Insect, M3i, Marques, metagenomic, virome
@article{Martins_2019jbio,
title = {Les insectes : un fantastique réservoir de virus et de gènes antiviraux},
author = {NE Martins and RP Olmo and ERGR Aguiar and JT Marques and JL Imler},
url = {https://www.biologie-journal.org/articles/jbio/abs/2018/02/jbio190008/jbio190008.html},
doi = {10.1051/jbio/2019008},
year = {2019},
date = {2019-04-11},
journal = {Biologie Aujourd'hui},
volume = {212},
number = {3-4},
pages = {101-106},
abstract = {Les insectes forment le groupe d’animaux qui présente la plus grande diversité. Des travaux récents de métagénomique montrent qu’ils peuvent être infectés par une diversité extraordinaire de virus. Parmi eux, les arbovirus (arthropod-borne viruses) peuvent être transmis à l’Homme par les insectes hématophages, notamment les moustiques. Le séquençage à haut débit des petits ARN des insectes fournit des informations sur leur virome, un paramètre qui pourrait contribuer à expliquer la dynamique de la transmission des maladies infectieuses par des insectes vecteurs. D’autre part, la caractérisation des mécanismes qui restreignent les infections virales chez les insectes révèle des innovations génétiques qui pourraient à terme inspirer de nouvelles stratégies antivirales.},
keywords = {antiviral immunity, ARN interference, imler, Insect, M3i, Marques, metagenomic, virome},
pubstate = {published},
tppubtype = {article}
}
Martins N, Lemoine A, Santiago E, Paro S, Imler JL, Meignin C
A Transgenic Flock House Virus Replicon Reveals an RNAi Independent Antiviral Mechanism Acting in Drosophila Follicular Somatic Cells Article de journal
Dans: G3 (Bethesda), vol. 9, no. 2, p. 403-412, 2019.
Liens | BibTeX | Étiquettes: antiviral immunity, Drosophila melanogaster, Follicular somatic cells, Genetics of Immunity, imler, M3i, meignin, replicon, Viral replicon
@article{Martins_2019i,
title = {A Transgenic Flock House Virus Replicon Reveals an RNAi Independent Antiviral Mechanism Acting in Drosophila Follicular Somatic Cells },
author = {N Martins and A Lemoine and E Santiago and S Paro and JL Imler and C Meignin},
url = {https://www.g3journal.org/content/9/2/403.long},
doi = {10.1534/g3.118.200872},
year = {2019},
date = {2019-02-07},
journal = { G3 (Bethesda)},
volume = {9},
number = {2},
pages = {403-412},
keywords = {antiviral immunity, Drosophila melanogaster, Follicular somatic cells, Genetics of Immunity, imler, M3i, meignin, replicon, Viral replicon},
pubstate = {published},
tppubtype = {article}
}
2016
Lamiable Olivier, Kellenberger Christine, Kemp Cordula, Troxler Laurent, Pelte Nadège, Boutros Michael, Marques Joao Trindade, Daeffler Laurent, Hoffmann Jules A, Roussel Alain, Imler Jean-Luc
Cytokine Diedel and a viral homologue suppress the IMD pathway in Drosophila Article de journal
Dans: PNAS, vol. 113, no. 3, p. 698–703, 2016, ISSN: 0027-8424, 1091-6490.
Résumé | Liens | BibTeX | Étiquettes: antiviral immunity, bioinformatic, cytokine, Edin, hoffmann, imler, M3i, Sindbis Virus, virokine
@article{lamiable_cytokine_2016,
title = {Cytokine Diedel and a viral homologue suppress the IMD pathway in Drosophila},
author = {Olivier Lamiable and Christine Kellenberger and Cordula Kemp and Laurent Troxler and Nadège Pelte and Michael Boutros and Joao Trindade Marques and Laurent Daeffler and Jules A Hoffmann and Alain Roussel and Jean-Luc Imler},
url = {http://www.pnas.org/content/113/3/698.abstract},
doi = {10.1073/pnas.1516122113},
issn = {0027-8424, 1091-6490},
year = {2016},
date = {2016-01-19},
urldate = {2016-01-07},
journal = {PNAS},
volume = {113},
number = {3},
pages = {698–703},
abstract = {Viruses are obligatory intracellular parasites that suffer strong evolutionary pressure from the host immune system. Rapidly evolving viral genomes can adapt to this pressure by acquiring genes that counteract host defense mechanisms. For example, many vertebrate DNA viruses have hijacked cellular genes encoding cytokines or cytokine receptors to disrupt host cell communication. Insect viruses express suppressors of RNA interference or apoptosis, highlighting the importance of these cell intrinsic antiviral mechanisms in invertebrates. Here, we report the identification and characterization of a family of proteins encoded by insect DNA viruses that are homologous to a 12-kDa circulating protein encoded by the virus-induced Drosophila gene diedel (die). We show that die mutant flies have shortened lifespan and succumb more rapidly than controls when infected with Sindbis virus. This reduced viability is associated with deregulated activation of the immune deficiency (IMD) pathway of host defense and can be rescued by mutations in the genes encoding the homolog of IKKγ or IMD itself. Our results reveal an endogenous pathway that is exploited by insect viruses to modulate NF-κB signaling and promote fly survival during the antiviral response.},
keywords = {antiviral immunity, bioinformatic, cytokine, Edin, hoffmann, imler, M3i, Sindbis Virus, virokine},
pubstate = {published},
tppubtype = {article}
}
Lamiable Olivier, Arnold Johan, da de Faria Isaque Joao Silva, Olmo Roenick Proveti, Bergami Francesco, Meignin Carine, Hoffmann Jules A, Marques Joao Trindade, Imler Jean-Luc
Analysis of the Contribution of Hemocytes and Autophagy to Drosophila Antiviral Immunity Article de journal
Dans: J. Virol., vol. 90, no. 11, p. 5415–5426, 2016, ISSN: 0022-538X, 1098-5514.
Liens | BibTeX | Étiquettes: antiviral immunity, Autophagy, Hemocytes, hoffmann, imler, M3i, meignin
@article{lamiable_analysis_2016,
title = {Analysis of the Contribution of Hemocytes and Autophagy to Drosophila Antiviral Immunity},
author = {Olivier Lamiable and Johan Arnold and Isaque Joao Silva da de Faria and Roenick Proveti Olmo and Francesco Bergami and Carine Meignin and Jules A Hoffmann and Joao Trindade Marques and Jean-Luc Imler},
url = {http://jvi.asm.org/content/90/11/5415},
doi = {10.1128/JVI.00238-16},
issn = {0022-538X, 1098-5514},
year = {2016},
date = {2016-01-01},
urldate = {2016-06-05},
journal = {J. Virol.},
volume = {90},
number = {11},
pages = {5415--5426},
keywords = {antiviral immunity, Autophagy, Hemocytes, hoffmann, imler, M3i, meignin},
pubstate = {published},
tppubtype = {article}
}
2012
Imler Jean-Luc, Hoffmann Jules A
Nucleic Acid Sensors and Antiviral Immunity Chapitre d'ouvrage
Dans: eds T. Fujita & P. Sambhara, (Ed.): Chapitre 1 : Antiviral responses in invertebrates, p. 1-18, Landes Bioscience, 2012.
BibTeX | Étiquettes: antiviral immunity, hoffmann, imler, Invertebrate, M3i
@inbook{Imler2012,
title = {Nucleic Acid Sensors and Antiviral Immunity},
author = {Jean-Luc Imler and Jules A Hoffmann},
editor = {eds T. Fujita & P. Sambhara},
year = {2012},
date = {2012-06-01},
pages = {1-18},
publisher = {Landes Bioscience},
chapter = {1 : Antiviral responses in invertebrates},
keywords = {antiviral immunity, hoffmann, imler, Invertebrate, M3i},
pubstate = {published},
tppubtype = {inbook}
}
2011
Chtarbanova Stanislava, Imler Jean-Luc
Immunité innée antivirale chez la drosophile Article de journal
Dans: Virologie, vol. 15, no. 5, p. 296-306, 2011, ISSN: 1267-8694.
Résumé | Liens | BibTeX | Étiquettes: *RNA Interference, antiviral immunity, Argonaute, dicer, helicase, imler, M3i, viruses
@article{Chtarbanova2011,
title = {Immunité innée antivirale chez la drosophile},
author = {Stanislava Chtarbanova and Jean-Luc Imler},
url = {http://www.jle.com/fr/revues/vir/e-docs/immunite_innee_antivirale_chez_la_drosophile_290366/article.phtml?tab=texte},
doi = {10.1684/vir.2011.0417},
issn = {1267-8694},
year = {2011},
date = {2011-10-01},
journal = {Virologie},
volume = {15},
number = {5},
pages = {296-306},
abstract = {La drosophile est utilisée depuis une quinzaine d’années comme modèle pour l’étude des mécanismes de l’immunité innée contre les infections bactériennes et fongiques. Les mécanismes de défense de cet insecte contre les infections virales sont maintenant abordés. L’interférence ARN joue un rôle essentiel dans la détection et le contrôle des virus. Ce mécanisme implique la reconnaissance des ARN double brins viraux par Dicer-2 et leur coupure pour former des petits ARN interférants (ARNsi) de 21 nucléotides. Ces ARNsi sont ensuite chargés sur l’enzyme Argonaute-2, qui inhibera spécifiquement les ARN viraux contenant une séquence complémentaire. Une réponse immunitaire inductible contribue également au contrôle des infections virales chez la drosophile. Cette réponse implique les voies de signalisation Toll et IMD, d’une part, (régulant des facteurs de transcription de la famille NF-κB) et JAK/STAT, d’autre part. On ne sait encore que peu de choses sur la nature des molécules effectrices régulées par ces voies de signalisation et sur les récepteurs qui les activent. Même si de nombreux points d’interrogation demeurent, l’intérêt du modèle drosophile pour identifier des mécanismes de défense antivirale conservés au cours de l’évolution, et impliqués dans la transmission des arbovirus par les moustiques, est aujourd’hui bien établi.},
keywords = {*RNA Interference, antiviral immunity, Argonaute, dicer, helicase, imler, M3i, viruses},
pubstate = {published},
tppubtype = {article}
}