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@article{,

title = {Growth kinetics, diffraction properties and effect of agarose on the stability of a novel crystal form of Thermus thermophilus aspartyl-tRNA synthetase-1},

author = {D W Zhu and B Lorber and C Sauter and J D Ng and P Benas and C Le Grimellec and R Giege},

url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11264584},

isbn = {11264584},

year  = {2001},

date = {2001-01-01},

journal = {Acta Crystallogr D Biol Crystallogr},

volume = {57},

number = {Pt 4},

pages = {552-558},

abstract = {Growth kinetics and diffraction properties of monoclinic crystals of eubacterial Thermus thermophilus aspartyl-tRNA synthetase-1 (AspRS-1) prepared in the presence of polyethylene glycol and agarose are studied. Their solubility and two-dimensional phase diagram are compared with those of orthorhombic crystals which grow in the presence of sodium formate or ammonium sulfate. The growth mechanism of the novel crystals was monitored by atomic force microscopy. The gel stabilizes the crystal lattice under the cryogenic conditions used for structure determination at high resolution.},

note = {0907-4449 

Journal Article},

keywords = {Aspartate-tRNA Ligase/*chemistry/*metabolism  Crystallization  Crystallography, Atomic Force  Osmolar Concentration  Sepharose/*metabolism  Solubility  Support, Non-U.S. Gov't  Temperature  Thermodynamics  Thermus thermophilus/*enzymology, SAUTER, Unité ARN, X-Ray/methods  Enzyme Stability  Gels  Kinetics  Microscopy},

pubstate = {published},

tppubtype = {article}

}