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Costa A., de Barros J. P. Pais, Keith G., Baranowski W., Desgres J.

Determination of queuosine derivatives by reverse-phase liquid chromatography for the hypomodification study of Q-bearing tRNAs from various mammal liver cells Article de journal

Dans: J Chromatogr B Analyt Technol Biomed Life Sci, vol. 801, no. 2, p. 237-47, 2004, (1570-0232 Journal Article).

Résumé | BibTeX | Étiquettes: *Chromatography, &, Acyl/chemistry, Amino, Animals, Asn/chemistry, Cells, Chickens, Cultured, derivatives/*analysis, Experimental, Gov't, Hepatocytes/chemistry, high, KEITH, liquid, Liver, Liver/*chemistry, Neoplasms, Non-U.S., Nucleoside, Pressure, purification, Q/*analogs, Rats, RNA, Support, Transfer, Transfer/*chemistry/isolation, tumor

@article{,

title = {Determination of queuosine derivatives by reverse-phase liquid chromatography for the hypomodification study of Q-bearing tRNAs from various mammal liver cells},

author = { A. Costa and J. P. Pais de Barros and G. Keith and W. Baranowski and J. Desgres},

year  = {2004},

date = {2004-01-01},

journal = {J Chromatogr B Analyt Technol Biomed Life Sci},

volume = {801},

number = {2},

pages = {237-47},

abstract = {Three queuosine derivatives (Q-derivatives) have been found at position 34 of four mammalian so-called Q-tRNAs: queuosine (Q) in tRNA(Asn) and tRNA(His), mannosyl-queuosine (manQ) in tRNA(Asp), and galactosyl-queuosine (galQ) in tRNA(Tyr). An analytical procedure based on the combined means of purified tRNA isolation from liver cells and ribonucleoside analysis by reverse-phase high performance liquid chromatography coupled with real-time UV-spectrometry (RPLC-UV) was developed for the quantitative analysis of the three Q-derivatives present in total tRNA from liver tissues and liver cell cultures. Using this analytical procedure, the rates of Q-tRNA modification were studied in total tRNAs from various mammalian hepatic cells. Our results show that the four Q-tRNAs are fully modified in liver tissues from adult mammals, regardless of the mammal species. However, a lack in the Q-modification level was observed in Q-tRNAs from newborn rat liver, as well in Q-tRNAs from normal rat liver cell cultures growing in a low queuine content medium, and from a rat hepatoma cell line. It is noteworthy that in all cases of Q-tRNA hypomodification, our analytical procedure showed that tRNA(Asp) is always the least affected by the hypomodification. The biological significance of this phenomenon is discussed.},

note = {1570-0232 

Journal Article},

keywords = {*Chromatography, &, Acyl/chemistry, Amino, Animals, Asn/chemistry, Cells, Chickens, Cultured, derivatives/*analysis, Experimental, Gov't, Hepatocytes/chemistry, high, KEITH, liquid, Liver, Liver/*chemistry, Neoplasms, Non-U.S., Nucleoside, Pressure, purification, Q/*analogs, Rats, RNA, Support, Transfer, Transfer/*chemistry/isolation, tumor},

pubstate = {published},

tppubtype = {article}

}

Fermer

Keith G.

Mobilities of modified ribonucleotides on two-dimensional cellulose thin-layer chromatography Article de journal

Dans: Biochimie, vol. 77, no. 1-2, p. 142-4, 1995, (0300-9084 Journal Article).

BibTeX | Étiquettes: *Chromatography, Layer, Purines/chemistry, Pyrimidines/chemistry, Ribonucleases/metabolism, Ribonucleotides/*chemistry, RNA, Thin, Transfer/chemistry