@inbook{,
title = {Modification of Selenoprotein mRNAs by Cap Tri-methylation},
author = {A S Gribling-Burrer and G Eriani and C Allmang},
editor = {L Chavatte},
url = {https://www.ncbi.nlm.nih.gov/pubmed/28917041?dopt=Abstract},
doi = {1007/978-1-4939-7258-6_9},
isbn = {28917041},
year = {2018},
date = {2018-01-01},
booktitle = {Selenoproteins: Methods and Protocols},
volume = {1661},
pages = {125-141},
publisher = {Springer Protocols, Humana Press},
series = {Methods in Molecular Biology},
abstract = {Several selenoprotein mRNAs undergo 5' cap maturation events whereby their classical monomethylated m7G cap becomes trimethylated (m32,2,7G) by the trimethylguanosine synthase 1 (Tgs1). Here, we describe immunoprecipitation methods for the detection of endogenous m32,2,7G-capped selenoprotein mRNAs from total cell extracts or after polysome fractionation of cytoplasmic extracts. We have also developed a method for the in vitro cap hypermethylation of selenoprotein mRNA transcripts using purified Tgs1 enzyme.},
keywords = {2, 7G cap, Cap hypermethylation Cap immunoprecipitation Selenoprotein mRNAs TMG cap Tgs1 m3 2, ERIANI, Unité ARN},
pubstate = {published},
tppubtype = {inbook}
}
Several selenoprotein mRNAs undergo 5' cap maturation events whereby their classical monomethylated m7G cap becomes trimethylated (m32,2,7G) by the trimethylguanosine synthase 1 (Tgs1). Here, we describe immunoprecipitation methods for the detection of endogenous m32,2,7G-capped selenoprotein mRNAs from total cell extracts or after polysome fractionation of cytoplasmic extracts. We have also developed a method for the in vitro cap hypermethylation of selenoprotein mRNA transcripts using purified Tgs1 enzyme.