Chagot M E, Quinternet M, Rothé B, Charpentier B, Coutant J, Manival X, Lebars I
The yeast C/D box snoRNA U14 adopts a Article de journal
Dans: Biochimie, vol. 164, p. 70-82, 2019, ISBN: 30914254.
Résumé | Liens | BibTeX | Étiquettes: ENNIFAR, K-turn NMR Snu13 protein snoRNA U14, Unité ARN
@article{,
title = {The yeast C/D box snoRNA U14 adopts a },
author = {M E Chagot and M Quinternet and B Rothé and B Charpentier and J Coutant and X Manival and I Lebars},
url = {https://www.ncbi.nlm.nih.gov/pubmed/30914254?dopt=Abstract},
doi = {10.1016/j.biochi.2019.03.014},
isbn = {30914254},
year = {2019},
date = {2019-01-01},
journal = {Biochimie},
volume = {164},
pages = {70-82},
abstract = {Non-coding RNAs associate with proteins to form ribonucleoproteins (RNPs), such as ribosome, box C/D snoRNPs, H/ACA snoRNPs, ribonuclease P, telomerase and spliceosome to ensure cell viability. The assembly of these RNA-protein complexes relies on the ability of the RNA to adopt the correct bound conformation. K-turn motifs represent ubiquitous binding platform for proteins found in several cellular environment. This structural motif has an internal three-nucleotide bulge flanked on its 3' side by a G●A/A●G tandem pairs followed by one or two non-Watson-Crick pairs, and on its 5' side by a classical RNA helix. This peculiar arrangement induces a strong curvature of the phosphodiester backbone, which makes it conducive to multiple tertiary interactions. SNU13/Snu13p (Human/Yeast) binds specifically the U14 C/D box snoRNA K-turn sequence motif. This event is the prerequisite to promote the assembly of the RNP, which contains NOP58/Nop58 and NOP56/Nop56 core proteins and the 2'-O-methyl-transferase, Fibrillarin/Nop1p. The U14 small nucleolar RNA is a conserved non-coding RNA found in yeast and vertebrates required for the pre-rRNA maturation and ribose methylation. Here, we report the solution structure of the free U14 snoRNA K-turn motif (kt-U14) as determined by Nuclear Magnetic Resonance. We demonstrate that a major fraction of free kt-U14 adopts a pre-folded conformation similar to protein bound K-turn, even in the absence of divalent ions. In contrast to the kt-U4 or tyrS RNA, kt-U14 displays a sharp bent in the phosphodiester backbone. The U●U and G●A tandem base pairs are formed through weak hydrogen bonds. Finally, we show that the structure of kt-U14 is stabilized upon Snu13p binding. The structure of the free U14 RNA is the first reference example for the canonical motifs of the C/D box snoRNA family.},
keywords = {ENNIFAR, K-turn NMR Snu13 protein snoRNA U14, Unité ARN},
pubstate = {published},
tppubtype = {article}
}