M3i

@article{,

title = {Toward predicting self-splicing and protein-facilitated splicing of group I introns},

author = {Q Vicens and P J Paukstelis and E Westhof and A M Lambowitz and T R Cech},

url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18768647},

isbn = {18768647},

year  = {2008},

date = {2008-01-01},

journal = {RNA},

volume = {14},

number = {10},

pages = {2013-2029},

abstract = {In the current era of massive discoveries of noncoding RNAs within genomes, being able to infer a function from a nucleotide sequence is of paramount interest. Although studies of individual group I introns have identified self-splicing and nonself-splicing examples, there is no overall understanding of the prevalence of self-splicing or the factors that determine it among the >2300 group I introns sequenced to date. Here, the self-splicing activities of 12 group I introns from various organisms were assayed under six reaction conditions that had been shown previously to promote RNA catalysis for different RNAs. Besides revealing that assessing self-splicing under only one condition can be misleading, this survey emphasizes that in vitro self-splicing efficiency is correlated with the GC content of the intron (>35% GC was generally conductive to self-splicing), and with the ability of the introns to form particular tertiary interactions. Addition of the Neurospora crassa CYT-18 protein activated splicing of two nonself-splicing introns, but inhibited the second step of self-splicing for two others. Together, correlations between sequence, predicted structure and splicing begin to establish rules that should facilitate our ability to predict the self-splicing activity of any group I intron from its sequence.},

note = {1469-9001 (Electronic) 

Journal Article 

Research Support, N.I.H., Extramural 

Research Support, Non-U.S. Gov't},

keywords = {Base Composition Base Sequence Catalysis *Introns Neurospora crassa/enzymology *Nucleic Acid Conformation *RNA Splicing RNA, Catalytic/*chemistry  RNA, Messenger/*chemistry  Tyrosine-tRNA Ligase/*chemistry, Unité ARN, WESTHOF, WESTHOF  Base Composition  Base Sequence  Catalysis  *Introns  Neurospora crassa/enzymology  *Nucleic Acid Conformation  *RNA Splicing  RNA},

pubstate = {published},

tppubtype = {article}

}