@article{,
title = {Activation of the catalytic core of a group I intron by a remote 3' splice junction},
author = {F Michel and L Jaeger and E Westhof and R Kuras and F Tihy and M Q Xu and D A Shub},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1644285},
isbn = {1644285},
year = {1992},
date = {1992-01-01},
journal = {Genes Dev},
volume = {6},
number = {8},
pages = {1373-1385},
abstract = {Over 1000 nucleotides may separate the ribozyme core of some group I introns from their 3' splice junctions. Using the sunY intron of bacteriophage T4 as a model system, we have investigated the mechanisms by which proximal splicing events are suppressed in vitro, as well as in vivo. Exon ligation as well as cleavage at the 5' splice site are shown to require long-range pairing between one of the peripheral components of the ribozyme core and some of the nucleotides preceding the authentic 3' splice junction. Consistent with our three-dimensional modeling of the entire sunY ribozyme, we propose that this novel interaction is necessary to drive 5' exon-core transcripts into an active conformation. A requirement for additional stabilizing interactions, either RNA-based or mediated by proteins, appears to be a general feature of group I self-splicing. A role for these interactions in mediating putative alternative splicing events is discussed.},
note = {0890-9369
Journal Article},
keywords = {Base Sequence DNA Mutational Analysis Escherichia coli/genetics Genes, Catalytic/genetics/*metabolism RNA, Messenger/genetics/*metabolism Support, P.H.S. T-Phages/genetics, U.S. Gov't, Unité ARN, Viral/*genetics Introns/genetics/*physiology Magnesium/metabolism Molecular Sequence Data Mutation/genetics Nucleic Acid Conformation Plasmids/genetics RNA Splicing/*genetics RNA},
pubstate = {published},
tppubtype = {article}
}
Over 1000 nucleotides may separate the ribozyme core of some group I introns from their 3' splice junctions. Using the sunY intron of bacteriophage T4 as a model system, we have investigated the mechanisms by which proximal splicing events are suppressed in vitro, as well as in vivo. Exon ligation as well as cleavage at the 5' splice site are shown to require long-range pairing between one of the peripheral components of the ribozyme core and some of the nucleotides preceding the authentic 3' splice junction. Consistent with our three-dimensional modeling of the entire sunY ribozyme, we propose that this novel interaction is necessary to drive 5' exon-core transcripts into an active conformation. A requirement for additional stabilizing interactions, either RNA-based or mediated by proteins, appears to be a general feature of group I self-splicing. A role for these interactions in mediating putative alternative splicing events is discussed.