M3i

@article{,

title = {The secondary structure of the 5'-noncoding region of beet necrotic yellow vein virus RNA 3: evidence for a role in viral RNA replication},

author = {D Gilmer and C Allmang and C Ehresmann and H Guilley and K Richards and G Jonard and B Ehresmann},

url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8464729},

isbn = {8464729},

year  = {1993},

date = {1993-01-01},

journal = {Nucleic Acids Res},

volume = {21},

number = {6},

pages = {1389-1395},

abstract = {Secondary structure-sensitive chemical and enzymatic probes have been used to produce a model for the folding of the first 312 residues of the long 5'-noncoding region of beet necrotic yellow vein virus RNA 3. The structure consists of two major domains, one of which includes long distance base-pairing interactions between two short sequence elements (Box I and Box II) situated between positions 237 and 292 and complementary elements (Box I' and II') near the 5'-terminus. Previous studies have shown that base pairing between these sequence elements (in either the plus-strand or minus-strand RNA) is important for RNA 3 accumulation during infection. RNA 3 transcripts were produced containing mutations which preferentially disrupted Box II-II' base pairing in either the plus- or minus-strand. In infection experiments, transcripts with mutations which disrupted the Box II-II' interaction in the plus-strand structure replicated less efficiently than mutants in which the Box II-II' interaction was disrupted in the minus-strand. These findings indicate that the complex 5'-proximal plus-strand structure to which the Box II-II' interaction contributes comprises at least part of the promoter for plus-strand RNA synthesis.},

note = {0305-1048 

Journal Article},

keywords = {Base Sequence  Gene Expression  Hydrogen Bonding  Molecular Sequence Data  Mutagenesis, Messenger/genetics  RNA, Site-Directed  Nucleic Acid Conformation  Plant Viruses/*genetics/ultrastructure  Promoter Regions (Genetics)  RNA Viruses/*genetics/ultrastructure  RNA, Unité ARN, Viral/chemistry/*genetics/ultrastructure  Vegetables  *Virus Replication},

pubstate = {published},

tppubtype = {article}

}