M3i

@article{,

title = {A single internal ribosome entry site containing a G quartet RNA structure drives fibroblast growth factor 2 gene expression at four alternative translation initiation codons},

author = {S Bonnal and C Schaeffer and L Creancier and S Clamens and H Moine and A C Prats and S Vagner},

url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12857733},

isbn = {12857733},

year  = {2003},

date = {2003-01-01},

journal = {J Biol Chem},

volume = {278},

number = {41},

pages = {39330-39336},

abstract = {The 484-nucleotide (nt) alternatively translated region (ATR) of the human fibroblast growth factor 2 (FGF-2) mRNA contains four CUG and one AUG translation initiation codons. Although the 5'-end proximal CUG codon is initiated by a cap-dependent translation process, the other four initiation codons are initiated by a mechanism of internal entry of ribosomes. We undertook here a detailed analysis of the cis-acting elements defining the FGF-2 internal ribosome entry site (IRES). A thorough deletion analysis study within the 5'-ATR led us to define a 176-nt region as being necessary and sufficient for IRES function at four codons present in a downstream 308-nt RNA segment. Unexpectedly, a single IRES module is therefore responsible for translation initiation at four distantly localized codons. The determination of the FGF-2 5'-ATR RNA secondary structure by enzymatic and chemical probing experiments showed that the FGF-2 IRES contained two stem-loop regions and a G quartet motif that constitute novel structural determinants of IRES function.},

note = {0021-9258 

Journal Article},

keywords = {Alternative Splicing  Base Sequence  Cell Line  Codon, Complementary/genetics  Fibroblast Growth Factor 2/*genetics  Gene Expression  Human  Molecular Sequence Data  Nucleic Acid Conformation  Peptide Chain Initiation  RNA, Initiator/genetics  DNA, Messenger/*chemistry/*genetics  Ribosomes/*metabolism  Sequence Deletion  Support, Non-U.S. Gov't  Transfection, Unité ARN},

pubstate = {published},

tppubtype = {article}

}