@article{,
title = {Mutational analysis of the pseudoknot structure of the S15 translational operator from Escherichia coli},
author = {L Benard and C Philippe and L Dondon and M Grunberg-Manago and B Ehresmann and C Ehresmann and C Portier},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7830558},
isbn = {7830558},
year = {1994},
date = {1994-01-01},
journal = {Mol Microbiol},
volume = {14},
number = {1},
pages = {31-40},
abstract = {Expression of rpsO, the gene encoding the small ribosomal protein S15, is autoregulated at the translational level by S15, which binds to its mRNA in a region overlapping the ribosome-binding site. By measuring the effect of mutations on the expression of a translational rpsO-lacZ fusion and the S15 binding affinity for the translational operator, the formation of a pseudoknot in the operator site in vivo is fully demonstrated and appears to be a prerequisite for S15 binding. The mutational analysis suggests also that specific determinants for S15 binding are located in very limited regions of the structure formed by the pseudoknot. It is deduced that a specific pseudoknot conformation is a key element for autoregulation.},
note = {0950-382x
Journal Article},
keywords = {Bacterial Molecular Sequence Data *Nucleic Acid Conformation Plasmids Protein Binding RNA, Bacteriophage lambda/genetics Base Sequence Binding Sites Codon DNA Mutational Analysis Escherichia coli/*genetics/metabolism *Gene Expression Regulation, Genetic beta-Galactosidase/biosynthesis, Genetic Translation, Messenger/*chemistry/*metabolism Recombinant Fusion Proteins/biosynthesis Restriction Mapping Ribosomal Proteins/biosynthesis/*genetics/metabolism Support, Non-U.S. Gov't Transcription, Unité ARN},
pubstate = {published},
tppubtype = {article}
}
Expression of rpsO, the gene encoding the small ribosomal protein S15, is autoregulated at the translational level by S15, which binds to its mRNA in a region overlapping the ribosome-binding site. By measuring the effect of mutations on the expression of a translational rpsO-lacZ fusion and the S15 binding affinity for the translational operator, the formation of a pseudoknot in the operator site in vivo is fully demonstrated and appears to be a prerequisite for S15 binding. The mutational analysis suggests also that specific determinants for S15 binding are located in very limited regions of the structure formed by the pseudoknot. It is deduced that a specific pseudoknot conformation is a key element for autoregulation.