Hoffmann Jules A, Kafatos Fotis C, Janeway Charles A, Ezekowitz Alan R B
Phylogenetic perspectives in innate immunity Article de journal
Dans: Science, vol. 284, no. 5418, p. 1313–1318, 1999, ISSN: 0036-8075.
Résumé | BibTeX | Étiquettes: Active, Animals, Culicidae, hoffmann, Humans, Immunity, Immunological, infection, Innate, Insect Vectors, M3i, Mammals, Models, Phagocytosis, Phylogeny, Proteins
@article{hoffmann_phylogenetic_1999,
title = {Phylogenetic perspectives in innate immunity},
author = {Jules A Hoffmann and Fotis C Kafatos and Charles A Janeway and Alan R B Ezekowitz},
issn = {0036-8075},
year = {1999},
date = {1999-05-01},
journal = {Science},
volume = {284},
number = {5418},
pages = {1313--1318},
abstract = {The concept of innate immunity refers to the first-line host defense that serves to limit infection in the early hours after exposure to microorganisms. Recent data have highlighted similarities between pathogen recognition, signaling pathways, and effector mechanisms of innate immunity in Drosophila and mammals, pointing to a common ancestry of these defenses. In addition to its role in the early phase of defense, innate immunity in mammals appears to play a key role in stimulating the subsequent, clonal response of adaptive immunity.},
keywords = {Active, Animals, Culicidae, hoffmann, Humans, Immunity, Immunological, infection, Innate, Insect Vectors, M3i, Mammals, Models, Phagocytosis, Phylogeny, Proteins},
pubstate = {published},
tppubtype = {article}
}
Lowenberger C A, Kamal S, Chiles J, Paskewitz S, Bulet Philippe, Hoffmann Jules A, Christensen B M
Mosquito-Plasmodium interactions in response to immune activation of the vector Article de journal
Dans: Exp. Parasitol., vol. 91, no. 1, p. 59–69, 1999, ISSN: 0014-4894.
Résumé | Liens | BibTeX | Étiquettes: Aedes, Animals, Anopheles, Culicidae, Defensins, Digestive System, Escherichia coli, Female, Genetic, Hemolymph, hoffmann, Insect Vectors, M3i, messenger, Micrococcus luteus, Plasmodium, Plasmodium berghei, Plasmodium gallinaceum, Proteins, Reverse Transcriptase Polymerase Chain Reaction, RNA, Transcription
@article{lowenberger_mosquito-plasmodium_1999,
title = {Mosquito-Plasmodium interactions in response to immune activation of the vector},
author = {C A Lowenberger and S Kamal and J Chiles and S Paskewitz and Philippe Bulet and Jules A Hoffmann and B M Christensen},
doi = {10.1006/expr.1999.4350},
issn = {0014-4894},
year = {1999},
date = {1999-01-01},
journal = {Exp. Parasitol.},
volume = {91},
number = {1},
pages = {59--69},
abstract = {During the development of Plasmodium sp. within the mosquito midgut, the parasite undergoes a series of developmental changes. The elongated ookinete migrates through the layers of the midgut where it forms the oocyst under the basal lamina. We demonstrate here that if Aedes aegypti or Anopheles gambiae, normally susceptible to Plasmodium gallinaceum and P. berghei, respectively, are immune activated by the injection of bacteria into the hemocoel, and subsequently are fed on an infectious bloodmeal, there is a significant reduction in the prevalence and mean intensity of infection of oocysts on the midgut. Only those mosquitoes immune activated prior to, or immediately after, parasite ingestion exhibit this reduction in parasite development. Mosquitoes immune activated 2-5 days after bloodfeeding show no differences in parasite burdens compared with naive controls. Northern analyses reveal that transcriptional activity for mosquito defensins is not detected in the whole bodies of Ae. aegypti from 4 h to 10 days after ingesting P. gallinaceum, suggesting that parasite ingestion, passage from the food bolus through the midgut, oocyst formation, and subsequent release of sporozoites into the hemolymph do not induce the production of defensin. However, reverse transcriptase-PCR of RNA isolated solely from the midguts of Ae. aegypti indicates that transcription of mosquito defensins occurs in the midguts of naive mosquitoes and those ingesting an infectious or noninfectious bloodmeal. Bacteria-challenged Ae. aegypti showed high levels of mature defensin in the hemolymph that correlate with a lower prevalence and mean intensity of infection with oocysts. Because few oocysts were found on the midgut of immune-activated mosquitoes, the data suggest that some factor, induced by bacterial challenge, kills the parasite at a preoocyst stage.},
keywords = {Aedes, Animals, Anopheles, Culicidae, Defensins, Digestive System, Escherichia coli, Female, Genetic, Hemolymph, hoffmann, Insect Vectors, M3i, messenger, Micrococcus luteus, Plasmodium, Plasmodium berghei, Plasmodium gallinaceum, Proteins, Reverse Transcriptase Polymerase Chain Reaction, RNA, Transcription},
pubstate = {published},
tppubtype = {article}
}
Lowenberger C A, Ferdig M T, Bulet Philippe, Khalili S, Hoffmann Jules A, Christensen B M
Aedes aegypti: induced antibacterial proteins reduce the establishment and development of Brugia malayi Article de journal
Dans: Exp. Parasitol., vol. 83, no. 2, p. 191–201, 1996, ISSN: 0014-4894.
Résumé | Liens | BibTeX | Étiquettes: Aedes, Analysis of Variance, Animals, Anti-Bacterial Agents, Base Sequence, Blood Proteins, Blotting, Brugia malayi, Culicidae, Defensins, DNA, Escherichia coli, Fat Body, Genetic, Gerbillinae, hoffmann, M3i, Micrococcus luteus, Microfilaria, Northern, RNA, Transcription
@article{lowenberger_aedes_1996,
title = {Aedes aegypti: induced antibacterial proteins reduce the establishment and development of Brugia malayi},
author = {C A Lowenberger and M T Ferdig and Philippe Bulet and S Khalili and Jules A Hoffmann and B M Christensen},
doi = {10.1006/expr.1996.0066},
issn = {0014-4894},
year = {1996},
date = {1996-07-01},
journal = {Exp. Parasitol.},
volume = {83},
number = {2},
pages = {191--201},
abstract = {The effect of host immune activation on the development of Brugia malayi in one susceptible and four refractory strains of Aedes aegypti and in Armigeres subalbatus was assessed. A. aegypti that were immune activated by the injection of saline or bacteria 24 hr before feeding on a B. malayi-infected gerbil had significantly reduced prevalences and mean intensities of infection from those of naive controls when exposed to bloodmeals with low (105 mf/20 microliters) and medium (160 mf/20 microliters) microfilaremias. At a higher microfilaremia (237 mf/20 microliters) there were no significant differences in mean intensities, suggesting that the number of parasites ingested may affect the host's ability to mount an effective defense response. Because the major immune proteins in A. aegypti are defensins, we did Northern analyses of fat body RNA 8 hr after immune activation or bloodfeeding. All mosquitoes demonstrated rapid transcriptional activity for defensins following immune activation by intrathoracic inoculation with either saline or bacteria. However, no strain of A. aegypti, susceptible or refractory to B. malayi, nor Ar. subalbatus produced defensin transcripts after bloodfeeding on an uninfected or a B. malayi-infected gerbil. These data suggest that inducible immune proteins of mosquitoes can reduce the prevalence and mean intensity of infections with ingested parasites, but these proteins are not expressed routinely after parasite ingestion and midgut penetration and probably do not contribute to existing refractory mechanisms. Immune proteins such as defensins, however, represent potential candidates to genetically engineer mosquitoes for resistance to filarial worms.},
keywords = {Aedes, Analysis of Variance, Animals, Anti-Bacterial Agents, Base Sequence, Blood Proteins, Blotting, Brugia malayi, Culicidae, Defensins, DNA, Escherichia coli, Fat Body, Genetic, Gerbillinae, hoffmann, M3i, Micrococcus luteus, Microfilaria, Northern, RNA, Transcription},
pubstate = {published},
tppubtype = {article}
}