Intranet
Acces
Contact
Accès direct
Unités de recherche
Bienvenue
M3i
Modèles Insectes d’Immunité Innée
Publications
2015
Aguiar Eric Roberto Guimarães Rocha, Olmo Roenick Proveti, Paro Simona, Ferreira Flavia Viana, da de Faria Isaque João Silva, Todjro Yaovi Mathias Honore, Lobo Francisco Pereira, Kroon Erna Geessien, Meignin Carine, Gatherer Derek, Imler Jean-Luc, Marques João Trindade
Sequence-independent characterization of viruses based on the pattern of viral small RNAs produced by the host Article de journal
Dans: Nucleic Acids Research, vol. 43, no. 13, p. 6191–6206, 2015, ISSN: 1362-4962.
Résumé | Liens | BibTeX | Étiquettes: Animals, Contig Mapping, Female, imler, insects, M3i, meignin, Ovary, Plants, RNA, Sequence Analysis, Small Untranslated, Vertebrates, Viral, Viral Tropism, viruses
@article{aguiar_sequence-independent_2015,
title = {Sequence-independent characterization of viruses based on the pattern of viral small RNAs produced by the host},
author = {Eric Roberto Guimarães Rocha Aguiar and Roenick Proveti Olmo and Simona Paro and Flavia Viana Ferreira and Isaque João Silva da de Faria and Yaovi Mathias Honore Todjro and Francisco Pereira Lobo and Erna Geessien Kroon and Carine Meignin and Derek Gatherer and Jean-Luc Imler and João Trindade Marques},
url = {http://nar.oxfordjournals.org/lookup/doi/10.1093/nar/gkv587},
doi = {10.1093/nar/gkv587},
issn = {1362-4962},
year = {2015},
date = {2015-07-01},
journal = {Nucleic Acids Research},
volume = {43},
number = {13},
pages = {6191--6206},
abstract = {Virus surveillance in vector insects is potentially of great benefit to public health. Large-scale sequencing of small and long RNAs has previously been used to detect viruses, but without any formal comparison of different strategies. Furthermore, the identification of viral sequences largely depends on similarity searches against reference databases. Here, we developed a sequence-independent strategy based on virus-derived small RNAs produced by the host response, such as the RNA interference pathway. In insects, we compared sequences of small and long RNAs, demonstrating that viral sequences are enriched in the small RNA fraction. We also noted that the small RNA size profile is a unique signature for each virus and can be used to identify novel viral sequences without known relatives in reference databases. Using this strategy, we characterized six novel viruses in the viromes of laboratory fruit flies and wild populations of two insect vectors: mosquitoes and sandflies. We also show that the small RNA profile could be used to infer viral tropism for ovaries among other aspects of virus biology. Additionally, our results suggest that virus detection utilizing small RNAs can also be applied to vertebrates, although not as efficiently as to plants and insects.},
keywords = {Animals, Contig Mapping, Female, imler, insects, M3i, meignin, Ovary, Plants, RNA, Sequence Analysis, Small Untranslated, Vertebrates, Viral, Viral Tropism, viruses},
pubstate = {published},
tppubtype = {article}
}
Virus surveillance in vector insects is potentially of great benefit to public health. Large-scale sequencing of small and long RNAs has previously been used to detect viruses, but without any formal comparison of different strategies. Furthermore, the identification of viral sequences largely depends on similarity searches against reference databases. Here, we developed a sequence-independent strategy based on virus-derived small RNAs produced by the host response, such as the RNA interference pathway. In insects, we compared sequences of small and long RNAs, demonstrating that viral sequences are enriched in the small RNA fraction. We also noted that the small RNA size profile is a unique signature for each virus and can be used to identify novel viral sequences without known relatives in reference databases. Using this strategy, we characterized six novel viruses in the viromes of laboratory fruit flies and wild populations of two insect vectors: mosquitoes and sandflies. We also show that the small RNA profile could be used to infer viral tropism for ovaries among other aspects of virus biology. Additionally, our results suggest that virus detection utilizing small RNAs can also be applied to vertebrates, although not as efficiently as to plants and insects.
1988
Lanot R, Thiebold J, Costet-Corio M F, Benveniste P, Hoffmann Jules A
Further experimental evidence for the involvement of ecdysone in the control of meiotic reinitiation in oocytes of Locusta migratoria (Insecta, Orthoptera) Article de journal
Dans: Dev. Biol., vol. 126, no. 1, p. 212–214, 1988, ISSN: 0012-1606.
Résumé | BibTeX | Étiquettes: Animals, Ecdysone, Female, Grasshoppers, hoffmann, M3i, Meiosis, Oocytes, Ovary
@article{lanot_further_1988,
title = {Further experimental evidence for the involvement of ecdysone in the control of meiotic reinitiation in oocytes of Locusta migratoria (Insecta, Orthoptera)},
author = {R Lanot and J Thiebold and M F Costet-Corio and P Benveniste and Jules A Hoffmann},
issn = {0012-1606},
year = {1988},
date = {1988-03-01},
journal = {Dev. Biol.},
volume = {126},
number = {1},
pages = {212--214},
abstract = {Ecdysone has recently been shown to be able to trigger meiotic reinitiation in vitro in submature oocytes of Locusta. In the present study we have experimentally depressed (by 60-70%) ecdysone biosynthesis in the ovaries of adult females by rearing them on a diet with a modified sterol profile. Mature oocytes from such females fail to undergo normal reinitiation, but when incubated in vitro, can be induced to break their meiotic arrest by the addition of exogenous ecdysone. These results lend further support to the hypothesis that in Locusta, ovarian ecdysone is involved in the control of meiotic reinitiation.},
keywords = {Animals, Ecdysone, Female, Grasshoppers, hoffmann, M3i, Meiosis, Oocytes, Ovary},
pubstate = {published},
tppubtype = {article}
}
Ecdysone has recently been shown to be able to trigger meiotic reinitiation in vitro in submature oocytes of Locusta. In the present study we have experimentally depressed (by 60-70%) ecdysone biosynthesis in the ovaries of adult females by rearing them on a diet with a modified sterol profile. Mature oocytes from such females fail to undergo normal reinitiation, but when incubated in vitro, can be induced to break their meiotic arrest by the addition of exogenous ecdysone. These results lend further support to the hypothesis that in Locusta, ovarian ecdysone is involved in the control of meiotic reinitiation.
1982
Hetru Charles, Kappler Christine, Hoffmann Jules A, Nearn R, Bang Lee, Horn D H
The biosynthetic pathway of ecdysone: studies with vitellogenic ovaries of Locusta migratoria (Orthoptera) Article de journal
Dans: Mol. Cell. Endocrinol., vol. 26, no. 1-2, p. 51–80, 1982, ISSN: 0303-7207.
Résumé | BibTeX | Étiquettes: Animals, Chromatography, Ecdysone, Female, Gas Chromatography-Mass Spectrometry, High Pressure Liquid, hoffmann, M3i, Orthoptera, Ovary
@article{hetru_biosynthetic_1982,
title = {The biosynthetic pathway of ecdysone: studies with vitellogenic ovaries of Locusta migratoria (Orthoptera)},
author = {Charles Hetru and Christine Kappler and Jules A Hoffmann and R Nearn and Lee Bang and D H Horn},
issn = {0303-7207},
year = {1982},
date = {1982-04-01},
journal = {Mol. Cell. Endocrinol.},
volume = {26},
number = {1-2},
pages = {51--80},
abstract = {Ovaries of adult females of Locusta migratoria synthesize impressive amounts of the steroid hormone ecdysone (and related ecdysteroids) during the late phases of vitellogenesis. The present study, aimed at elucidating the sequence of the biosynthetic steps that lead from cholesterol to ecdysone, has taken benefit of this remarkable biological model by using a double approach: (1) isolation and physico-chemical identification of endogenous biogenetic intermediates; (2) metabolic study of labelled putative precursor molecules. The data presented in this paper lead us to propose the following sequence of events: conversion of cholesterol to 3 beta-hydroxy-5 beta-cholest-7-en-6-one (via several intermediates not identified in this study) followed by 14 beta-hydroxylation to 3 beta, 14 alpha-dihydroxy-5 beta-cholest-7-en-6-one; hydroxylation on the side-chain at C-25 and C-22 (in this order) to 2-deoxyecdysone; hydroxylation at C-2 to ecdysone.},
keywords = {Animals, Chromatography, Ecdysone, Female, Gas Chromatography-Mass Spectrometry, High Pressure Liquid, hoffmann, M3i, Orthoptera, Ovary},
pubstate = {published},
tppubtype = {article}
}
Ovaries of adult females of Locusta migratoria synthesize impressive amounts of the steroid hormone ecdysone (and related ecdysteroids) during the late phases of vitellogenesis. The present study, aimed at elucidating the sequence of the biosynthetic steps that lead from cholesterol to ecdysone, has taken benefit of this remarkable biological model by using a double approach: (1) isolation and physico-chemical identification of endogenous biogenetic intermediates; (2) metabolic study of labelled putative precursor molecules. The data presented in this paper lead us to propose the following sequence of events: conversion of cholesterol to 3 beta-hydroxy-5 beta-cholest-7-en-6-one (via several intermediates not identified in this study) followed by 14 beta-hydroxylation to 3 beta, 14 alpha-dihydroxy-5 beta-cholest-7-en-6-one; hydroxylation on the side-chain at C-25 and C-22 (in this order) to 2-deoxyecdysone; hydroxylation at C-2 to ecdysone.
1981
Lachaise F, Goudeau M, Hetru Charles, Kappler Christine, Hoffmann Jules A
Ecdysteroids and ovarian development in the shore crab, Carcinus maenas Article de journal
Dans: Hoppe-Seyler's Z. Physiol. Chem., vol. 362, no. 5, p. 521–529, 1981, ISSN: 0018-4888.
Résumé | BibTeX | Étiquettes: aging, Animals, Brachyura, Cross Reactions, Ecdysone, Ecdysterone, Female, hoffmann, M3i, Ovary, Radioimmunoassay, Sexual Maturation
@article{lachaise_ecdysteroids_1981,
title = {Ecdysteroids and ovarian development in the shore crab, Carcinus maenas},
author = {F Lachaise and M Goudeau and Charles Hetru and Christine Kappler and Jules A Hoffmann},
issn = {0018-4888},
year = {1981},
date = {1981-01-01},
journal = {Hoppe-Seyler's Z. Physiol. Chem.},
volume = {362},
number = {5},
pages = {521--529},
abstract = {Mature ovaries of the shore crab Carcinus maenas contain large concentrations of three major ecdysteroids which we have identified by physicochemical methods as ecdysone, 20-hydroxyecdysone and ponasterone A. The fluctuations of ovarian and blood ecdysteroid concentrations are presented in relation to the various stages of ovarian development.},
keywords = {aging, Animals, Brachyura, Cross Reactions, Ecdysone, Ecdysterone, Female, hoffmann, M3i, Ovary, Radioimmunoassay, Sexual Maturation},
pubstate = {published},
tppubtype = {article}
}
Mature ovaries of the shore crab Carcinus maenas contain large concentrations of three major ecdysteroids which we have identified by physicochemical methods as ecdysone, 20-hydroxyecdysone and ponasterone A. The fluctuations of ovarian and blood ecdysteroid concentrations are presented in relation to the various stages of ovarian development.
1978
Goltzené F, Lagueux Marie, Charlet Maurice, Hoffmann Jules A
The follicle cell epithelium of maturing ovaries of Locusta migratoria: a new biosynthetic tissue for ecdysone Article de journal
Dans: Hoppe-Seyler's Z. Physiol. Chem., vol. 359, no. 10, p. 1427–1434, 1978, ISSN: 0018-4888.
Résumé | BibTeX | Étiquettes: Animals, Ecdysone, Epithelium, Female, Grasshoppers, hoffmann, Kinetics, M3i, Oocytes, Ovarian Follicle, Ovary, Sexual Maturation
@article{goltzene_follicle_1978,
title = {The follicle cell epithelium of maturing ovaries of Locusta migratoria: a new biosynthetic tissue for ecdysone},
author = {F Goltzené and Marie Lagueux and Maurice Charlet and Jules A Hoffmann},
issn = {0018-4888},
year = {1978},
date = {1978-10-01},
journal = {Hoppe-Seyler's Z. Physiol. Chem.},
volume = {359},
number = {10},
pages = {1427--1434},
abstract = {Follicle cells of maturing ovaries of Locusta migratoria are demonstrated to synthesize the moulting hormone ecdysone (2beta,3beta,14alpha,22R,25-pentahydroxy-5beta-cholest-7-en-6-one). Studies of secretory kinetics under in vitro conditions show that the intensity of hormone secretion is strictly dependent on the stage of maturation of the excised ovaries.},
keywords = {Animals, Ecdysone, Epithelium, Female, Grasshoppers, hoffmann, Kinetics, M3i, Oocytes, Ovarian Follicle, Ovary, Sexual Maturation},
pubstate = {published},
tppubtype = {article}
}
Follicle cells of maturing ovaries of Locusta migratoria are demonstrated to synthesize the moulting hormone ecdysone (2beta,3beta,14alpha,22R,25-pentahydroxy-5beta-cholest-7-en-6-one). Studies of secretory kinetics under in vitro conditions show that the intensity of hormone secretion is strictly dependent on the stage of maturation of the excised ovaries.
Hetru Charles, Lagueux Marie, Bang L, Hoffmann Jules A
Adult ovaries of Locusta migratoria contain the sequence of biosynthetic intermediates for ecdysone Article de journal
Dans: Life Sci., vol. 22, no. 23, p. 2141–2154, 1978, ISSN: 0024-3205.
BibTeX | Étiquettes: Animals, Chemical Phenomena, Chemistry, Cholesterol, Ecdysone, Female, Grasshoppers, hoffmann, M3i, Mass Spectrometry, Ovary, Ovum
@article{hetru_adult_1978,
title = {Adult ovaries of Locusta migratoria contain the sequence of biosynthetic intermediates for ecdysone},
author = {Charles Hetru and Marie Lagueux and L Bang and Jules A Hoffmann},
issn = {0024-3205},
year = {1978},
date = {1978-06-01},
journal = {Life Sci.},
volume = {22},
number = {23},
pages = {2141--2154},
keywords = {Animals, Chemical Phenomena, Chemistry, Cholesterol, Ecdysone, Female, Grasshoppers, hoffmann, M3i, Mass Spectrometry, Ovary, Ovum},
pubstate = {published},
tppubtype = {article}
}
1977
Lagueux Marie, Hirn M, Hoffmann Jules A
Ecdysone during ovarian development in Locusta migratoria Article de journal
Dans: J. Insect Physiol., vol. 23, no. 1, p. 109–119, 1977, ISSN: 0022-1910.
BibTeX | Étiquettes: Animals, Ecdysone, Female, Grasshoppers, hoffmann, M3i, Male, Oogenesis, Ovary
@article{lagueux_ecdysone_1977,
title = {Ecdysone during ovarian development in Locusta migratoria},
author = {Marie Lagueux and M Hirn and Jules A Hoffmann},
issn = {0022-1910},
year = {1977},
date = {1977-01-01},
journal = {J. Insect Physiol.},
volume = {23},
number = {1},
pages = {109--119},
keywords = {Animals, Ecdysone, Female, Grasshoppers, hoffmann, M3i, Male, Oogenesis, Ovary},
pubstate = {published},
tppubtype = {article}
}
1976
Bang L, Lagueux Marie, Hirn M, Hoffmann Jules A
Identification, by gas chromatography and mass spectrometry, of ecdysone synthesized in the ovaries of adult female Locusta migratoria (Insecta, Orthoptera) Article de journal
Dans: C.R. Hebd. Seances Acad. Sci., Ser. D, Sci. Nat., vol. 283, no. 9, p. 1081–1084, 1976.
Résumé | BibTeX | Étiquettes: Age Factors, Animals, Chromatography, Ecdysone, Female, Gas, Grasshoppers, hoffmann, M3i, Mass Spectrometry, Ovary
@article{bang_identification_1976,
title = {Identification, by gas chromatography and mass spectrometry, of ecdysone synthesized in the ovaries of adult female Locusta migratoria (Insecta, Orthoptera)},
author = {L Bang and Marie Lagueux and M Hirn and Jules A Hoffmann},
year = {1976},
date = {1976-10-01},
journal = {C.R. Hebd. Seances Acad. Sci., Ser. D, Sci. Nat.},
volume = {283},
number = {9},
pages = {1081--1084},
abstract = {Gas chromatographic analysis, coupled to mass spectrometry, demonstrates that the considerable amounts of ecdysteroids produced in the ovaries of female adults of Locusta migratoria at the end of each ovarian cycle, consist predominantly of ecdysone.},
keywords = {Age Factors, Animals, Chromatography, Ecdysone, Female, Gas, Grasshoppers, hoffmann, M3i, Mass Spectrometry, Ovary},
pubstate = {published},
tppubtype = {article}
}
Gas chromatographic analysis, coupled to mass spectrometry, demonstrates that the considerable amounts of ecdysteroids produced in the ovaries of female adults of Locusta migratoria at the end of each ovarian cycle, consist predominantly of ecdysone.
Lagueux Marie, Hirn M, de Reggi M, Hoffmann Jules A
Ecdysteroid levels and ovarian development in adult female Locust migratoria Article de journal
Dans: C.R. Hebd. Seances Acad. Sci., Ser. D, Sci. Nat., vol. 282, no. 12, p. 1187–1190, 1976.
Résumé | BibTeX | Étiquettes: Adipose Tissue, Animals, Castration, Female, Grasshoppers, Hemolymph, hoffmann, Invertebrate Hormones, M3i, Neurosecretory Systems, Oogenesis, Ovary, Oviposition
@article{lagueux_ecdysteroid_1976,
title = {Ecdysteroid levels and ovarian development in adult female Locust migratoria},
author = {Marie Lagueux and M Hirn and M de Reggi and Jules A Hoffmann},
year = {1976},
date = {1976-03-01},
journal = {C.R. Hebd. Seances Acad. Sci., Ser. D, Sci. Nat.},
volume = {282},
number = {12},
pages = {1187--1190},
abstract = {During ovarian development in adult females of Locusta, an important biosynthesis of ecdysteroids is observed at the end of the maturation process of the terminal oocyte. This phenomenon occurs regularly during the different ovarian cycles. No synthesis is observed in allatectomized or ovariectomized female adults.},
keywords = {Adipose Tissue, Animals, Castration, Female, Grasshoppers, Hemolymph, hoffmann, Invertebrate Hormones, M3i, Neurosecretory Systems, Oogenesis, Ovary, Oviposition},
pubstate = {published},
tppubtype = {article}
}
During ovarian development in adult females of Locusta, an important biosynthesis of ecdysteroids is observed at the end of the maturation process of the terminal oocyte. This phenomenon occurs regularly during the different ovarian cycles. No synthesis is observed in allatectomized or ovariectomized female adults.