M3i

@article{,

title = {A sequence immediately upstream of the plus-strand primer is essential for plus-strand DNA synthesis of the Saccharomyces cerevisiae Ty1 retrotransposon},

author = { M. Wilhelm and T. Heyman and M. Boutabout and F. X. Wilhelm},

year  = {1999},

date = {1999-01-01},

journal = {Nucleic Acids Res},

volume = {27},

number = {23},

pages = {4547-52},

abstract = {Priming of plus-strand DNA is a critical step in reverse transcription of retroviruses and retrotransposons. All retroelements use an RNase H-resistant oligoribonucleotide spanning a purine-rich sequence (the polypurine tract or PPT) to prime plus-strand DNA synthesis. Plus-strand DNA synthesis of the yeast Saccharomyces cerevisiae Ty1-H3 retrotransposon is initiated at two sites, PPT1 and PPT2, located at the upstream boundary of the 3'-long terminal repeat and near the middle of the pol gene in the integrase coding region. The two plus-strand primers have the same purine-rich sequence GGGTGGTA. This sequence is not sufficient by itself to generate a plus-strand origin since two identical sequences located upstream of PPT2 in the integrase coding region are not used efficiently as primers for plus-strand DNA synthesis. Thus, other factors must be involved in the formation of a specific plus-strand DNA primer. We show here that mutations upstream of the PPT in a highly conserved T-rich region severely alters plus-strand DNA priming of Ty1. Our results demonstrate the importance of sequences or structural elements upstream of the PPT for initiation of plus-strand DNA synthesis.},

note = {0305-1048 

Journal Article},

keywords = {*DNA, *Retroelements, Base, cerevisiae/*genetics, DNA, Elements, Fungal/*biosynthesis, Gov't, Mutation, Non-U.S., Primers, response, Saccharomyces, Sequence, Support},

pubstate = {published},

tppubtype = {article}

}