Publications
2007
Marzi S, Myasnikov A G, Serganov A, Ehresmann C, Romby P, Yusupov M, Klaholz B P
Structured mRNAs regulate translation initiation by binding to the platform of the ribosome Article de journal
Dans: Cell, vol. 130, no. 6, p. 1019-31, 2007, ISBN: 17889647, (0092-8674 (Print) Comparative Study Journal Article Research Support, Non-U.S. Gov't).
Résumé | Liens | BibTeX | Étiquettes: 5' Untranslated Regions Amino Acid Sequence Base Sequence Binding Sites Cryoelectron Microscopy Escherichia coli/*genetics/metabolism Escherichia coli Proteins/chemistry/genetics/*metabolism *Gene Expression Regulation, Amino Acid Sequence Homology, Bacterial Models, Bacterial/chemistry/*metabolism RNA, Messenger/*metabolism RNA, Molecular Molecular Sequence Data Mutation Nucleic Acid Conformation *Peptide Chain Initiation, Nucleic Acid Structural Homology, Protein Time Factors, Ribonucleic Acid Ribosomal Proteins/chemistry/genetics/*metabolism Ribosomes/chemistry/*metabolism/ultrastructure Sequence Homology, ROMBY, Transfer/metabolism Regulatory Sequences, Translational Protein Binding Protein Conformation RNA, Unité ARN
@article{,
title = {Structured mRNAs regulate translation initiation by binding to the platform of the ribosome},
author = {S Marzi and A G Myasnikov and A Serganov and C Ehresmann and P Romby and M Yusupov and B P Klaholz},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17889647},
isbn = {17889647},
year = {2007},
date = {2007-01-01},
journal = {Cell},
volume = {130},
number = {6},
pages = {1019-31},
abstract = {Gene expression can be regulated at the level of initiation of protein biosynthesis via structural elements present at the 5' untranslated region of mRNAs. These folded mRNA segments may bind to the ribosome, thus blocking translation until the mRNA unfolds. Here, we report a series of cryo-electron microscopy snapshots of ribosomal complexes directly visualizing either the mRNA structure blocked by repressor protein S15 or the unfolded, active mRNA. In the stalled state, the folded mRNA prevents the start codon from reaching the peptidyl-tRNA (P) site inside the ribosome. Upon repressor release, the mRNA unfolds and moves into the mRNA channel allowing translation initiation. A comparative structure and sequence analysis suggests the existence of a universal stand-by site on the ribosome (the 30S platform) dedicated for binding regulatory 5' mRNA elements. Different types of mRNA structures may be accommodated during translation preinitiation and regulate gene expression by transiently stalling the ribosome.},
note = {0092-8674 (Print)
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't},
keywords = {5' Untranslated Regions Amino Acid Sequence Base Sequence Binding Sites Cryoelectron Microscopy Escherichia coli/*genetics/metabolism Escherichia coli Proteins/chemistry/genetics/*metabolism *Gene Expression Regulation, Amino Acid Sequence Homology, Bacterial Models, Bacterial/chemistry/*metabolism RNA, Messenger/*metabolism RNA, Molecular Molecular Sequence Data Mutation Nucleic Acid Conformation *Peptide Chain Initiation, Nucleic Acid Structural Homology, Protein Time Factors, Ribonucleic Acid Ribosomal Proteins/chemistry/genetics/*metabolism Ribosomes/chemistry/*metabolism/ultrastructure Sequence Homology, ROMBY, Transfer/metabolism Regulatory Sequences, Translational Protein Binding Protein Conformation RNA, Unité ARN},
pubstate = {published},
tppubtype = {article}
}