Réponses antivirales chez le moustique Aedes

@article{,

title = {Tertiary network in mammalian mitochondrial tRNAAsp revealed by solution probing and phylogeny},

author = {M Messmer and J Putz and T Suzuki and C Sauter and M Sissler and C Florentz},

url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19767615},

isbn = {19767615},

year  = {2009},

date = {2009-01-01},

journal = {Nucleic Acids Res},

volume = {37},

number = {20},

pages = {6881-6895},

abstract = {Primary and secondary structures of mammalian mitochondrial (mt) tRNAs are divergent from canonical tRNA structures due to highly skewed nucleotide content and large size variability of D- and T-loops. The nonconservation of nucleotides involved in the expected network of tertiary interactions calls into question the rules governing a functional L-shaped three-dimensional (3D) structure. Here, we report the solution structure of human mt-tRNA(Asp) in its native post-transcriptionally modified form and as an in vitro transcript. Probing performed with nuclease S1, ribonuclease V1, dimethylsulfate, diethylpyrocarbonate and lead, revealed several secondary structures for the in vitro transcribed mt-tRNA(Asp) including predominantly the cloverleaf. On the contrary, the native tRNA(Asp) folds into a single cloverleaf structure, highlighting the contribution of the four newly identified post-transcriptional modifications to correct folding. Reactivities of nucleotides and phosphodiester bonds in the native tRNA favor existence of a full set of six classical tertiary interactions between the D-domain and the variable region, forming the core of the 3D structure. Reactivities of D- and T-loop nucleotides support an absence of interactions between these domains. According to multiple sequence alignments and search for conservation of Leontis-Westhof interactions, the tertiary network core building rules apply to all tRNA(Asp) from mammalian mitochondria.},

note = {1362-4962 (Electronic) 

0305-1048 (Linking) 

Journal Article 

Research Support, Non-U.S. Gov't},

keywords = {Asp/*chemistry/*metabolism  Transcription, Base Sequence Databases, FLORENTZ, FRUGIER, Genetic, Nucleic Acid  Humans  Molecular Sequence Data  Nucleic Acid Conformation  Phylogeny  RNA/*chemistry/*metabolism  RNA, SISSLER, Transfer, Unité ARN},

pubstate = {published},

tppubtype = {article}

}