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2018
Rodrigues Artur Filipe, Newman Leon, Jasim Dhifaf A, Vacchi Isabella A, Ménard-Moyon Cécilia, Crica Livia E, Bianco Alberto, Kostarelos Kostas, Bussy Cyrill
Immunological impact of graphene oxide sheets in the abdominal cavity is governed by surface reactivity Article de journal
Dans: Archives of Toxicology, vol. 92, no. 11, p. 3359–3379, 2018, ISSN: 1432-0738.
Résumé | Liens | BibTeX | Étiquettes: 2D Materials, Animals, carbon, Epithelium, Female, graphene oxide, Graphite, I2CT, In vivo, Inbred C57BL, inflammation, Intraperitoneal, Macrophages, Mesothelium, Mice, Nanotubes, Peritoneal, Peritoneal Cavity, Protein coating, Team-Bianco, Tissue Distribution, Toxicity
@article{rodrigues_immunological_2018,
title = {Immunological impact of graphene oxide sheets in the abdominal cavity is governed by surface reactivity},
author = {Artur Filipe Rodrigues and Leon Newman and Dhifaf A Jasim and Isabella A Vacchi and Cécilia Ménard-Moyon and Livia E Crica and Alberto Bianco and Kostas Kostarelos and Cyrill Bussy},
doi = {10.1007/s00204-018-2303-z},
issn = {1432-0738},
year = {2018},
date = {2018-01-01},
journal = {Archives of Toxicology},
volume = {92},
number = {11},
pages = {3359--3379},
abstract = {Graphene oxide (GO) is an oxidised form of graphene that has attracted commercial interest in multiple applications, including inks, printed electronics and spray coatings, which all raise health concerns due to potential creation of inhalable aerosols. Although a number of studies have discussed the toxicity of GO sheets, the in vivo impact of their lateral dimensions is still not clear. Here, we compared the effects of large GO sheets (l-GO, 1-20 µm) with those of small GO sheets (s-GO, textbackslashtextless 1 µm) in terms of mesothelial damage and peritoneal inflammation, after intraperitoneal (i.p.) injection in mice. To benchmark the outcomes, long and rigid multi-walled carbon nanotubes (MWCNTs) that were shown to be associated with asbestos-like pathogenicity on the mesothelium were also tested. Our aim was to assess whether lateral dimensions can be a predictor of inflammogenicity for GO sheets in a similar fashion as length is for MWCNTs. While long MWCNTs dispersed in 0.5% BSA induced a granulomatous response on the diaphragmatic mesothelium and immune cell recruitment to the peritoneal cavity, GO sheets dispersed under similar conditions did not cause any response, regardless of their lateral dimensions. We further interrogated whether tuning the surface reactivity of GO by testing different dispersions (5% dextrose instead of 0.5% BSA) may change the biological outcome. Although the change of dispersion did not alter the impact of GO on the mesothelium (i.e. no granuloma), we observed that, when dispersed in protein-free 5% dextrose solution, s-GO elicited a greater recruitment of monocytic cells to the peritoneal cavity than l-GO, or when dispersed in protein-containing solution. Such recruitment coincided with the greater ability of s-GO to interact in vivo with peritoneal macrophages and was associated with a greater surface reactivity in comparison to l-GO. In conclusion, large dimension was not a determining factor of the immunological impact of GO sheets after i.p. administration. For an equal dose, GO sheets with lateral dimensions similar to the length of long MWCNTs were less pathogenic than the MWCNTs. On the other hand, surface reactivity and the ability of some smaller GO sheets to interact more readily with immune cells seem to be key parameters that can be tuned to improve the safety profile of GO. In particular, the choice of dispersion modality, which affected these two parameters, was found to be of crucial importance in the assessment of GO impact in this model. Overall, these findings are essential for a better understanding of the parameters governing GO toxicity and inflammation, and the rational design of safe GO-based formulations for various applications, including biomedicine.},
keywords = {2D Materials, Animals, carbon, Epithelium, Female, graphene oxide, Graphite, I2CT, In vivo, Inbred C57BL, inflammation, Intraperitoneal, Macrophages, Mesothelium, Mice, Nanotubes, Peritoneal, Peritoneal Cavity, Protein coating, Team-Bianco, Tissue Distribution, Toxicity},
pubstate = {published},
tppubtype = {article}
}
Graphene oxide (GO) is an oxidised form of graphene that has attracted commercial interest in multiple applications, including inks, printed electronics and spray coatings, which all raise health concerns due to potential creation of inhalable aerosols. Although a number of studies have discussed the toxicity of GO sheets, the in vivo impact of their lateral dimensions is still not clear. Here, we compared the effects of large GO sheets (l-GO, 1-20 µm) with those of small GO sheets (s-GO, textbackslashtextless 1 µm) in terms of mesothelial damage and peritoneal inflammation, after intraperitoneal (i.p.) injection in mice. To benchmark the outcomes, long and rigid multi-walled carbon nanotubes (MWCNTs) that were shown to be associated with asbestos-like pathogenicity on the mesothelium were also tested. Our aim was to assess whether lateral dimensions can be a predictor of inflammogenicity for GO sheets in a similar fashion as length is for MWCNTs. While long MWCNTs dispersed in 0.5% BSA induced a granulomatous response on the diaphragmatic mesothelium and immune cell recruitment to the peritoneal cavity, GO sheets dispersed under similar conditions did not cause any response, regardless of their lateral dimensions. We further interrogated whether tuning the surface reactivity of GO by testing different dispersions (5% dextrose instead of 0.5% BSA) may change the biological outcome. Although the change of dispersion did not alter the impact of GO on the mesothelium (i.e. no granuloma), we observed that, when dispersed in protein-free 5% dextrose solution, s-GO elicited a greater recruitment of monocytic cells to the peritoneal cavity than l-GO, or when dispersed in protein-containing solution. Such recruitment coincided with the greater ability of s-GO to interact in vivo with peritoneal macrophages and was associated with a greater surface reactivity in comparison to l-GO. In conclusion, large dimension was not a determining factor of the immunological impact of GO sheets after i.p. administration. For an equal dose, GO sheets with lateral dimensions similar to the length of long MWCNTs were less pathogenic than the MWCNTs. On the other hand, surface reactivity and the ability of some smaller GO sheets to interact more readily with immune cells seem to be key parameters that can be tuned to improve the safety profile of GO. In particular, the choice of dispersion modality, which affected these two parameters, was found to be of crucial importance in the assessment of GO impact in this model. Overall, these findings are essential for a better understanding of the parameters governing GO toxicity and inflammation, and the rational design of safe GO-based formulations for various applications, including biomedicine.
Arosio Paolo, Comito Giuseppina, Orsini Francesco, Lascialfari Alessandro, Chiarugi Paola, Ménard-Moyon Cécilia, Nativi Cristina, Richichi Barbara
Conjugation of a GM3 lactone mimetic on carbon nanotubes enhances the related inhibition of melanoma-associated metastatic events Article de journal
Dans: Organic & Biomolecular Chemistry, vol. 16, no. 33, p. 6086–6095, 2018, ISSN: 1477-0539.
Résumé | Liens | BibTeX | Étiquettes: Antineoplastic Agents, Biomimetic Materials, carbon, Cell Line, G(M3) Ganglioside, Humans, I2CT, Melanoma, Models, Molecular, Molecular Conformation, Nanotubes, Neoplasm Metastasis, Team-Bianco, tumor
@article{arosio_conjugation_2018,
title = {Conjugation of a GM3 lactone mimetic on carbon nanotubes enhances the related inhibition of melanoma-associated metastatic events},
author = {Paolo Arosio and Giuseppina Comito and Francesco Orsini and Alessandro Lascialfari and Paola Chiarugi and Cécilia Ménard-Moyon and Cristina Nativi and Barbara Richichi},
doi = {10.1039/c8ob01817k},
issn = {1477-0539},
year = {2018},
date = {2018-01-01},
journal = {Organic & Biomolecular Chemistry},
volume = {16},
number = {33},
pages = {6086--6095},
abstract = {GM3-ganglioside is known to be involved in melanoma proliferation. In order to modulate metastatic-related events, we have functionalized multi-walled carbon nanotubes (MWCNTs) with multiple copies of a GM3-lactone mimetic. The MWCNTs proved to guarantee the appropriate spatial arrangement of the mimetic allowing a stronger inhibition of migration and invasiveness of human melanoma (A375) cells compared to other multivalent constructs reported before. In addition, the effect of the multivalent tubular conjugate on the inhibition of specific tyrosine kinases, which are associated with the ganglioside complexes within the membrane domains, was demonstrated. Finally, the short-term fate of the conjugate was assessed, for the first time, by means of the 1H NMR relaxometry technique by exploiting the signal arising from the CNTs.},
keywords = {Antineoplastic Agents, Biomimetic Materials, carbon, Cell Line, G(M3) Ganglioside, Humans, I2CT, Melanoma, Models, Molecular, Molecular Conformation, Nanotubes, Neoplasm Metastasis, Team-Bianco, tumor},
pubstate = {published},
tppubtype = {article}
}
GM3-ganglioside is known to be involved in melanoma proliferation. In order to modulate metastatic-related events, we have functionalized multi-walled carbon nanotubes (MWCNTs) with multiple copies of a GM3-lactone mimetic. The MWCNTs proved to guarantee the appropriate spatial arrangement of the mimetic allowing a stronger inhibition of migration and invasiveness of human melanoma (A375) cells compared to other multivalent constructs reported before. In addition, the effect of the multivalent tubular conjugate on the inhibition of specific tyrosine kinases, which are associated with the ganglioside complexes within the membrane domains, was demonstrated. Finally, the short-term fate of the conjugate was assessed, for the first time, by means of the 1H NMR relaxometry technique by exploiting the signal arising from the CNTs.
2013
Dumortier Hélène
When carbon nanotubes encounter the immune system: desirable and undesirable effects Article de journal
Dans: Advanced Drug Delivery Reviews, vol. 65, no. 15, p. 2120–2126, 2013, ISSN: 1872-8294.
Résumé | Liens | BibTeX | Étiquettes: Animals, Biomedical application, carbon, Carbon nanotubes, Dumortier, Environmental Exposure, Functionalization, Humans, I2CT, Immune cell activation, Immune System, inflammation, Inhalation Exposure, Lymphocyte, Macrophage, Nanotubes, Occupational Exposure, Team-Dumortier, Toxicity
@article{dumortier_when_2013,
title = {When carbon nanotubes encounter the immune system: desirable and undesirable effects},
author = {Hélène Dumortier},
doi = {10.1016/j.addr.2013.09.005},
issn = {1872-8294},
year = {2013},
date = {2013-01-01},
journal = {Advanced Drug Delivery Reviews},
volume = {65},
number = {15},
pages = {2120--2126},
abstract = {The role of our immune system is to bring efficient protection against invasion by foreign elements, not only pathogens but also any material it may be in contact with. Nanoparticles may enter the body and encounter the immune system either intentionally (e.g. administration for biomedical application) or not (e.g. respiratory occupational exposure). Therefore, it is of fundamental importance to get a thorough knowledge of the way they interact with immune cells and all related consequences. Among nanomaterials, carbon nanotubes (CNTs) are of special interest because of their tremendous field of applications. Consequently, their increasing production, processing and eventual incorporation into new types of composites and/or into biological systems have raised fundamental issues regarding their potential impact on health. This review aims at giving an overview of the known desirable and undesirable effects of CNTs on the immune system, i.e. beneficial modulation of immune cells by CNTs engineered for biomedical applications versus toxicity, inflammation and unwanted immune reactions triggered by CNTs themselves.},
keywords = {Animals, Biomedical application, carbon, Carbon nanotubes, Dumortier, Environmental Exposure, Functionalization, Humans, I2CT, Immune cell activation, Immune System, inflammation, Inhalation Exposure, Lymphocyte, Macrophage, Nanotubes, Occupational Exposure, Team-Dumortier, Toxicity},
pubstate = {published},
tppubtype = {article}
}
The role of our immune system is to bring efficient protection against invasion by foreign elements, not only pathogens but also any material it may be in contact with. Nanoparticles may enter the body and encounter the immune system either intentionally (e.g. administration for biomedical application) or not (e.g. respiratory occupational exposure). Therefore, it is of fundamental importance to get a thorough knowledge of the way they interact with immune cells and all related consequences. Among nanomaterials, carbon nanotubes (CNTs) are of special interest because of their tremendous field of applications. Consequently, their increasing production, processing and eventual incorporation into new types of composites and/or into biological systems have raised fundamental issues regarding their potential impact on health. This review aims at giving an overview of the known desirable and undesirable effects of CNTs on the immune system, i.e. beneficial modulation of immune cells by CNTs engineered for biomedical applications versus toxicity, inflammation and unwanted immune reactions triggered by CNTs themselves.
2012
Delogu Lucia Gemma, Venturelli Enrica, Manetti Roberto, Pinna Gérard Aimé, Carru Ciriaco, Madeddu Roberto, Murgia Luciano, Sgarrella Francesco, Dumortier Hélène, Bianco Alberto
Ex vivo impact of functionalized carbon nanotubes on human immune cells Article de journal
Dans: Nanomedicine (London, England), vol. 7, no. 2, p. 231–243, 2012, ISSN: 1748-6963.
Résumé | Liens | BibTeX | Étiquettes: carbon, Cells, Cultured, Cytokines, Dumortier, Humans, I2CT, Immunity, Innate, Materials Testing, Nanotubes, T-Lymphocytes, Team-Bianco, Team-Dumortier
@article{delogu_ex_2012,
title = {Ex vivo impact of functionalized carbon nanotubes on human immune cells},
author = {Lucia Gemma Delogu and Enrica Venturelli and Roberto Manetti and Gérard Aimé Pinna and Ciriaco Carru and Roberto Madeddu and Luciano Murgia and Francesco Sgarrella and Hélène Dumortier and Alberto Bianco},
doi = {10.2217/nnm.11.101},
issn = {1748-6963},
year = {2012},
date = {2012-02-01},
journal = {Nanomedicine (London, England)},
volume = {7},
number = {2},
pages = {231--243},
abstract = {AIM: Different studies, carried out by us and others, have investigated the impact of carbon nanotubes (CNTs) in vitro and in animal models. To date, only a few studies have been performed on human cells ex vivo. There is also a lack of comparison between CNTs with varied functionalization and structural properties and their impact on different cell types.
MATERIALS & METHODS: The present ex vivo human study focuses on the impact of a series of functionalized multiwalled CNTs on human T and B lymphocytes, natural killer (NK) cells and monocytes.
RESULTS: Smaller diameter nanotubes are internalized more efficiently. Viability assays displayed the absence of cytotoxicity of all multiwalled CNTs used. Activation assay demonstrated a strong effect on monocytes and NK cells.
CONCLUSION: Our results, on human cells ex vivo, confirmed previous studies demonstrating appropriately functionalized CNTs are nontoxic. The effects on cell functionality were significant for the monocytes and NK cells. These findings encourage the possible use of CNTs for biomedical applications either as carriers of therapeutic molecules or as immune modulator systems.},
keywords = {carbon, Cells, Cultured, Cytokines, Dumortier, Humans, I2CT, Immunity, Innate, Materials Testing, Nanotubes, T-Lymphocytes, Team-Bianco, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}
AIM: Different studies, carried out by us and others, have investigated the impact of carbon nanotubes (CNTs) in vitro and in animal models. To date, only a few studies have been performed on human cells ex vivo. There is also a lack of comparison between CNTs with varied functionalization and structural properties and their impact on different cell types.
MATERIALS & METHODS: The present ex vivo human study focuses on the impact of a series of functionalized multiwalled CNTs on human T and B lymphocytes, natural killer (NK) cells and monocytes.
RESULTS: Smaller diameter nanotubes are internalized more efficiently. Viability assays displayed the absence of cytotoxicity of all multiwalled CNTs used. Activation assay demonstrated a strong effect on monocytes and NK cells.
CONCLUSION: Our results, on human cells ex vivo, confirmed previous studies demonstrating appropriately functionalized CNTs are nontoxic. The effects on cell functionality were significant for the monocytes and NK cells. These findings encourage the possible use of CNTs for biomedical applications either as carriers of therapeutic molecules or as immune modulator systems.
MATERIALS & METHODS: The present ex vivo human study focuses on the impact of a series of functionalized multiwalled CNTs on human T and B lymphocytes, natural killer (NK) cells and monocytes.
RESULTS: Smaller diameter nanotubes are internalized more efficiently. Viability assays displayed the absence of cytotoxicity of all multiwalled CNTs used. Activation assay demonstrated a strong effect on monocytes and NK cells.
CONCLUSION: Our results, on human cells ex vivo, confirmed previous studies demonstrating appropriately functionalized CNTs are nontoxic. The effects on cell functionality were significant for the monocytes and NK cells. These findings encourage the possible use of CNTs for biomedical applications either as carriers of therapeutic molecules or as immune modulator systems.
Serag Maged F, Kaji Noritada, Tokeshi Manabu, Bianco Alberto, Baba Yoshinobu
The plant cell uses carbon nanotubes to build tracheary elements Article de journal
Dans: Integrative Biology: Quantitative Biosciences from Nano to Macro, vol. 4, no. 2, p. 127–131, 2012, ISSN: 1757-9708.
Résumé | Liens | BibTeX | Étiquettes: Arabidopsis, Atomic Force, carbon, Cell Differentiation, Confocal, Endocytosis, I2CT, Lignin, Microscopy, Nanotubes, Plant Cells, Team-Bianco
@article{serag_plant_2012,
title = {The plant cell uses carbon nanotubes to build tracheary elements},
author = {Maged F Serag and Noritada Kaji and Manabu Tokeshi and Alberto Bianco and Yoshinobu Baba},
doi = {10.1039/c2ib00135g},
issn = {1757-9708},
year = {2012},
date = {2012-02-01},
journal = {Integrative Biology: Quantitative Biosciences from Nano to Macro},
volume = {4},
number = {2},
pages = {127--131},
abstract = {Since their discovery, carbon nanotubes (CNTs) have been eminent members of the nanomaterial family. Because of their unique physical, chemical and mechanical properties, they are regarded as new potential materials to bring enormous benefits in cell biology studies. Undoubtedly, the first step to prove the advantages of CNTs is to understand the basic behavior of CNTs inside the cells. In a number of studies, CNTs have been demonstrated as new carrier systems for the delivery of DNA, proteins and therapeutic molecules into living cells. However, post-uptake behavior of CNTs inside the cells has not received much consideration. Utilizing the plant cell model, we have shown in this study that the plant cells, differentiating into tracheary elements, incorporate cup-stacked carbon nanotubes (CSCNTs) into cell structure via oxidative cross-linking of monolignols to the nanotubes surface during lignin biosynthesis. This finding highlights the fate of CNTs inside plant cells and provides an example on how the plant cell can handle internalized carbon nanomaterials.},
keywords = {Arabidopsis, Atomic Force, carbon, Cell Differentiation, Confocal, Endocytosis, I2CT, Lignin, Microscopy, Nanotubes, Plant Cells, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Since their discovery, carbon nanotubes (CNTs) have been eminent members of the nanomaterial family. Because of their unique physical, chemical and mechanical properties, they are regarded as new potential materials to bring enormous benefits in cell biology studies. Undoubtedly, the first step to prove the advantages of CNTs is to understand the basic behavior of CNTs inside the cells. In a number of studies, CNTs have been demonstrated as new carrier systems for the delivery of DNA, proteins and therapeutic molecules into living cells. However, post-uptake behavior of CNTs inside the cells has not received much consideration. Utilizing the plant cell model, we have shown in this study that the plant cells, differentiating into tracheary elements, incorporate cup-stacked carbon nanotubes (CSCNTs) into cell structure via oxidative cross-linking of monolignols to the nanotubes surface during lignin biosynthesis. This finding highlights the fate of CNTs inside plant cells and provides an example on how the plant cell can handle internalized carbon nanomaterials.
Lacerda Lara, Russier Julie, Pastorin Giorgia, Herrero Antonia M, Venturelli Enrica, Dumortier Hélène, Al-Jamal Khuloud T, Prato Maurizio, Kostarelos Kostas, Bianco Alberto
Translocation mechanisms of chemically functionalised carbon nanotubes across plasma membranes Article de journal
Dans: Biomaterials, vol. 33, no. 11, p. 3334–3343, 2012, ISSN: 1878-5905.
Résumé | Liens | BibTeX | Étiquettes: Animals, carbon, Cell Line, Cell Membrane, Dumortier, I2CT, Macrophages, Mice, Nanotubes, Phagocytosis, Team-Bianco, Team-Dumortier
@article{lacerda_translocation_2012,
title = {Translocation mechanisms of chemically functionalised carbon nanotubes across plasma membranes},
author = {Lara Lacerda and Julie Russier and Giorgia Pastorin and Antonia M Herrero and Enrica Venturelli and Hélène Dumortier and Khuloud T Al-Jamal and Maurizio Prato and Kostas Kostarelos and Alberto Bianco},
doi = {10.1016/j.biomaterials.2012.01.024},
issn = {1878-5905},
year = {2012},
date = {2012-01-01},
journal = {Biomaterials},
volume = {33},
number = {11},
pages = {3334--3343},
abstract = {Understanding the mechanisms responsible for carbon nanotube (CNT) internalisation into live cells is considered critical both from a fundamental point of view and for further engineering of CNT-based delivery systems to intracellular targets. While several studies are focused on the development of such CNT-based delivery systems, attempts to systematically elucidate the cellular uptake mechanisms of CNTs are still rather limited. The aim of the present study is to evaluate the cellular internalisation of chemically functionalised multi-walled carbon nanotubes (f-MWCNTs) in the presence of different well-known cellular uptake inhibitors. Our data reveal how f-MWCNTs are able to translocate across cell membranes of both phagocytic and non-phagocytic cell lines. We have evidenced that at least 30-50% of f-MWCNTs are taken up by cells through an energy-independent mechanism. This characteristic makes nanotubes loaded with therapeutic or diagnostic cargos extremely interesting as the release of active molecules directly into the cytoplasm increase their biological activity and therapeutic efficacy.},
keywords = {Animals, carbon, Cell Line, Cell Membrane, Dumortier, I2CT, Macrophages, Mice, Nanotubes, Phagocytosis, Team-Bianco, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}
Understanding the mechanisms responsible for carbon nanotube (CNT) internalisation into live cells is considered critical both from a fundamental point of view and for further engineering of CNT-based delivery systems to intracellular targets. While several studies are focused on the development of such CNT-based delivery systems, attempts to systematically elucidate the cellular uptake mechanisms of CNTs are still rather limited. The aim of the present study is to evaluate the cellular internalisation of chemically functionalised multi-walled carbon nanotubes (f-MWCNTs) in the presence of different well-known cellular uptake inhibitors. Our data reveal how f-MWCNTs are able to translocate across cell membranes of both phagocytic and non-phagocytic cell lines. We have evidenced that at least 30-50% of f-MWCNTs are taken up by cells through an energy-independent mechanism. This characteristic makes nanotubes loaded with therapeutic or diagnostic cargos extremely interesting as the release of active molecules directly into the cytoplasm increase their biological activity and therapeutic efficacy.
2011
Serag Maged F, Kaji Noritada, Venturelli Enrica, Okamoto Yukihiro, Terasaka Kazuyoshi, Tokeshi Manabu, Mizukami Hajime, Braeckmans Kevin, Bianco Alberto, Baba Yoshinobu
Functional platform for controlled subcellular distribution of carbon nanotubes Article de journal
Dans: ACS nano, vol. 5, no. 11, p. 9264–9270, 2011, ISSN: 1936-086X.
Résumé | Liens | BibTeX | Étiquettes: Biological Transport, carbon, Catharanthus, Exocytosis, Fluorescence Recovery After Photobleaching, Fluorescent Dyes, I2CT, Intracellular Space, Nanotubes, Surface Properties, Team-Bianco, Vacuoles
@article{serag_functional_2011,
title = {Functional platform for controlled subcellular distribution of carbon nanotubes},
author = {Maged F Serag and Noritada Kaji and Enrica Venturelli and Yukihiro Okamoto and Kazuyoshi Terasaka and Manabu Tokeshi and Hajime Mizukami and Kevin Braeckmans and Alberto Bianco and Yoshinobu Baba},
doi = {10.1021/nn2035654},
issn = {1936-086X},
year = {2011},
date = {2011-11-01},
journal = {ACS nano},
volume = {5},
number = {11},
pages = {9264--9270},
abstract = {As nanoparticles can cross different cellular barriers and access different tissues, control of their uptake and cellular fate presents a functional approach that will be broadly applicable to nanoscale technologies in cell biology. Here we show that the trafficking of single-walled carbon nanotubes (SWCNTs) through various subcellular membranes of the plant cell is facilitated or inhibited by attaching a suitable functional tag and controlling medium components. This enables a unique control over the uptake and the subcellular distribution of SWCNTs and provides a key strategy to promote their cellular elimination to minimize toxicity. Our results also demonstrate that SWCNTs are involved in a carrier-mediated transport (CMT) inside cells; this is a phenomenon that scientists could use to obtain novel molecular insights into CMT, with the potential translation to advances in subcellular nanobiology.},
keywords = {Biological Transport, carbon, Catharanthus, Exocytosis, Fluorescence Recovery After Photobleaching, Fluorescent Dyes, I2CT, Intracellular Space, Nanotubes, Surface Properties, Team-Bianco, Vacuoles},
pubstate = {published},
tppubtype = {article}
}
As nanoparticles can cross different cellular barriers and access different tissues, control of their uptake and cellular fate presents a functional approach that will be broadly applicable to nanoscale technologies in cell biology. Here we show that the trafficking of single-walled carbon nanotubes (SWCNTs) through various subcellular membranes of the plant cell is facilitated or inhibited by attaching a suitable functional tag and controlling medium components. This enables a unique control over the uptake and the subcellular distribution of SWCNTs and provides a key strategy to promote their cellular elimination to minimize toxicity. Our results also demonstrate that SWCNTs are involved in a carrier-mediated transport (CMT) inside cells; this is a phenomenon that scientists could use to obtain novel molecular insights into CMT, with the potential translation to advances in subcellular nanobiology.
Ali-Boucetta Hanene, Al-Jamal Khuloud T, Müller Karin H, Li Shouping, Porter Alexandra E, Eddaoudi Ayad, Prato Maurizio, Bianco Alberto, Kostarelos Kostas
Cellular uptake and cytotoxic impact of chemically functionalized and polymer-coated carbon nanotubes Article de journal
Dans: Small (Weinheim an Der Bergstrasse, Germany), vol. 7, no. 22, p. 3230–3238, 2011, ISSN: 1613-6829.
Résumé | Liens | BibTeX | Étiquettes: Annexin A5, carbon, Cell Death, Cell Line, Endocytosis, Flow Cytometry, Fluorescein-5-isothiocyanate, Humans, I2CT, L-Lactate Dehydrogenase, mitochondria, Nanotubes, Polymers, Propidium, Surface Properties, Team-Bianco, tumor, water
@article{ali-boucetta_cellular_2011,
title = {Cellular uptake and cytotoxic impact of chemically functionalized and polymer-coated carbon nanotubes},
author = {Hanene Ali-Boucetta and Khuloud T Al-Jamal and Karin H Müller and Shouping Li and Alexandra E Porter and Ayad Eddaoudi and Maurizio Prato and Alberto Bianco and Kostas Kostarelos},
doi = {10.1002/smll.201101004},
issn = {1613-6829},
year = {2011},
date = {2011-11-01},
journal = {Small (Weinheim an Der Bergstrasse, Germany)},
volume = {7},
number = {22},
pages = {3230--3238},
abstract = {The impact of nanomaterials such as carbon nanotubes on biological matter is a topic of increasing interest and concern and requires a multifaceted approach to be resolved. A modified cytotoxic (lactate dehydrogenase (LDH)) assay is developed in an attempt to offer a valid and reliable methodology for screening carbon nanotube toxicity in vitro. Two of the most widely used types of surface-modified multiwalled carbon nanotubes (MWNTs) are tested: ammonium-functionalized MWNTs (MWNT-NH3+ ) and Pluronic F127 coated MWNTs (MWNT:F127). Chemically functionalized MWNTs show significantly greater cellular uptake into lung epithelial A549 cells compared to the non-covalently Pluronic F127-coated MWNTs. In spite of this, MWNT:F127 exhibit enhanced cytotoxicity according to the modified LDH assay. The validity of the modified LDH assay is further validated by direct comparison with other less reliable or accurate cytotoxicity assays. These findings indicate the reliability of the modified LDH assay as a screening tool to assess carbon nanotube cytotoxicity and illustrate that high levels of carbon nanotube cellular internalization do not necessarily lead to adverse responses.},
keywords = {Annexin A5, carbon, Cell Death, Cell Line, Endocytosis, Flow Cytometry, Fluorescein-5-isothiocyanate, Humans, I2CT, L-Lactate Dehydrogenase, mitochondria, Nanotubes, Polymers, Propidium, Surface Properties, Team-Bianco, tumor, water},
pubstate = {published},
tppubtype = {article}
}
The impact of nanomaterials such as carbon nanotubes on biological matter is a topic of increasing interest and concern and requires a multifaceted approach to be resolved. A modified cytotoxic (lactate dehydrogenase (LDH)) assay is developed in an attempt to offer a valid and reliable methodology for screening carbon nanotube toxicity in vitro. Two of the most widely used types of surface-modified multiwalled carbon nanotubes (MWNTs) are tested: ammonium-functionalized MWNTs (MWNT-NH3+ ) and Pluronic F127 coated MWNTs (MWNT:F127). Chemically functionalized MWNTs show significantly greater cellular uptake into lung epithelial A549 cells compared to the non-covalently Pluronic F127-coated MWNTs. In spite of this, MWNT:F127 exhibit enhanced cytotoxicity according to the modified LDH assay. The validity of the modified LDH assay is further validated by direct comparison with other less reliable or accurate cytotoxicity assays. These findings indicate the reliability of the modified LDH assay as a screening tool to assess carbon nanotube cytotoxicity and illustrate that high levels of carbon nanotube cellular internalization do not necessarily lead to adverse responses.
Singh Prabhpreet, Lamanna Giuseppe, Ménard-Moyon Cécilia, Toma Francesca Maria, Magnano Elena, Bondino Federica, Prato Maurizio, Verma Sandeep, Bianco Alberto
Formation of efficient catalytic silver nanoparticles on carbon nanotubes by adenine functionalization Article de journal
Dans: Angewandte Chemie (International Ed. in English), vol. 50, no. 42, p. 9893–9897, 2011, ISSN: 1521-3773.
Résumé | Liens | BibTeX | Étiquettes: Adenine, carbon, Catalysis, I2CT, Metal Nanoparticles, Molecular Structure, Nanotubes, Silver, Surface Properties, Team-Bianco
@article{singh_formation_2011,
title = {Formation of efficient catalytic silver nanoparticles on carbon nanotubes by adenine functionalization},
author = {Prabhpreet Singh and Giuseppe Lamanna and Cécilia Ménard-Moyon and Francesca Maria Toma and Elena Magnano and Federica Bondino and Maurizio Prato and Sandeep Verma and Alberto Bianco},
doi = {10.1002/anie.201102976},
issn = {1521-3773},
year = {2011},
date = {2011-10-01},
journal = {Angewandte Chemie (International Ed. in English)},
volume = {50},
number = {42},
pages = {9893--9897},
abstract = {Stuck together: adenine/carbon nanotube hybrids trigger the formation of controlled-size catalytic silver nanoparticles on the nanotube surface. The catalytic efficiency of the resulting species was assessed in the oxidation of 2-methylhydroquinone to its corresponding benzoquinone, with complete recovery and without loss of activity of the catalyst.},
keywords = {Adenine, carbon, Catalysis, I2CT, Metal Nanoparticles, Molecular Structure, Nanotubes, Silver, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Stuck together: adenine/carbon nanotube hybrids trigger the formation of controlled-size catalytic silver nanoparticles on the nanotube surface. The catalytic efficiency of the resulting species was assessed in the oxidation of 2-methylhydroquinone to its corresponding benzoquinone, with complete recovery and without loss of activity of the catalyst.
Bianco Alberto, Kostarelos Kostas, Prato Maurizio
Making carbon nanotubes biocompatible and biodegradable Article de journal
Dans: Chemical Communications (Cambridge, England), vol. 47, no. 37, p. 10182–10188, 2011, ISSN: 1364-548X.
Résumé | Liens | BibTeX | Étiquettes: Animals, Biocompatible, carbon, Coated Materials, HeLa Cells, Humans, I2CT, nanotechnology, Nanotubes, Team-Bianco
@article{bianco_making_2011,
title = {Making carbon nanotubes biocompatible and biodegradable},
author = {Alberto Bianco and Kostas Kostarelos and Maurizio Prato},
doi = {10.1039/c1cc13011k},
issn = {1364-548X},
year = {2011},
date = {2011-10-01},
journal = {Chemical Communications (Cambridge, England)},
volume = {47},
number = {37},
pages = {10182--10188},
abstract = {Carbon nanotubes are promising nanomaterials with great potential in the field of nanomedicine for both therapeutic and diagnostic applications. Different approaches have been developed to render this material biocompatible and to modulate any ensuing toxic effects. In the context of medical use, although chemically functionalised carbon nanotubes display reduced toxicity, they are still considered with scepticism due to their perceived non-biodegradability. Recently, it has been demonstrated that functionalised carbon nanotubes can be degraded by oxidative enzymes. This finding is offering a new perspective for the development of carbon nanotubes in medicine. This article highlights recent advances that can act as paradigm-shifts towards the design of biocompatible and biodegradable functionalised carbon nanotubes and allow their translation into the clinic.},
keywords = {Animals, Biocompatible, carbon, Coated Materials, HeLa Cells, Humans, I2CT, nanotechnology, Nanotubes, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes are promising nanomaterials with great potential in the field of nanomedicine for both therapeutic and diagnostic applications. Different approaches have been developed to render this material biocompatible and to modulate any ensuing toxic effects. In the context of medical use, although chemically functionalised carbon nanotubes display reduced toxicity, they are still considered with scepticism due to their perceived non-biodegradability. Recently, it has been demonstrated that functionalised carbon nanotubes can be degraded by oxidative enzymes. This finding is offering a new perspective for the development of carbon nanotubes in medicine. This article highlights recent advances that can act as paradigm-shifts towards the design of biocompatible and biodegradable functionalised carbon nanotubes and allow their translation into the clinic.
Venturelli Enrica, Fabbro Chiara, Chaloin Olivier, Ménard-Moyon Cécilia, Smulski Cristian R, Ros Tatiana Da, Kostarelos Kostas, Prato Maurizio, Bianco Alberto
Antibody covalent immobilization on carbon nanotubes and assessment of antigen binding Article de journal
Dans: Small (Weinheim an Der Bergstrasse, Germany), vol. 7, no. 15, p. 2179–2187, 2011, ISSN: 1613-6829.
Résumé | Liens | BibTeX | Étiquettes: Antibodies, Antigens, carbon, I2CT, Immobilized, Mucin-1, nanotechnology, Nanotubes, Protein Binding, Team-Bianco, Thermogravimetry
@article{venturelli_antibody_2011,
title = {Antibody covalent immobilization on carbon nanotubes and assessment of antigen binding},
author = {Enrica Venturelli and Chiara Fabbro and Olivier Chaloin and Cécilia Ménard-Moyon and Cristian R Smulski and Tatiana Da Ros and Kostas Kostarelos and Maurizio Prato and Alberto Bianco},
doi = {10.1002/smll.201100137},
issn = {1613-6829},
year = {2011},
date = {2011-08-01},
journal = {Small (Weinheim an Der Bergstrasse, Germany)},
volume = {7},
number = {15},
pages = {2179--2187},
abstract = {Controlling the covalent bonding of antibodies onto functionalized carbon nanotubes is a key step in the design and preparation of nanotube-based conjugates for targeting cancer cells. For this purpose, an anti-MUC1 antibody (Ab) is linked to both multi-walled (MWCNTs) and double-walled carbon nanotubes (DWCNTs) using different synthetic strategies. The presence of the Ab attached to the nanotubes is confirmed by gel electrophoresis and thermogravimetric analysis. Most importantly, molecular recognition of the antigen by surface plasmon resonance is able to determine similar Ab binding capacities for both Ab-DWCNTs and Ab-MWCNTs. These results are very relevant for the design of future receptor-targeting strategies using chemically functionalized carbon nanotubes.},
keywords = {Antibodies, Antigens, carbon, I2CT, Immobilized, Mucin-1, nanotechnology, Nanotubes, Protein Binding, Team-Bianco, Thermogravimetry},
pubstate = {published},
tppubtype = {article}
}
Controlling the covalent bonding of antibodies onto functionalized carbon nanotubes is a key step in the design and preparation of nanotube-based conjugates for targeting cancer cells. For this purpose, an anti-MUC1 antibody (Ab) is linked to both multi-walled (MWCNTs) and double-walled carbon nanotubes (DWCNTs) using different synthetic strategies. The presence of the Ab attached to the nanotubes is confirmed by gel electrophoresis and thermogravimetric analysis. Most importantly, molecular recognition of the antigen by surface plasmon resonance is able to determine similar Ab binding capacities for both Ab-DWCNTs and Ab-MWCNTs. These results are very relevant for the design of future receptor-targeting strategies using chemically functionalized carbon nanotubes.
Al-Jamal Khuloud T, Gherardini Lisa, Bardi Giuseppe, Nunes Antonio, Guo Chang, Bussy Cyrill, Herrero Antonia M, Bianco Alberto, Prato Maurizio, Kostarelos Kostas, Pizzorusso Tommaso
Functional motor recovery from brain ischemic insult by carbon nanotube-mediated siRNA silencing Article de journal
Dans: Proceedings of the National Academy of Sciences of the United States of America, vol. 108, no. 27, p. 10952–10957, 2011, ISSN: 1091-6490.
Résumé | Liens | BibTeX | Étiquettes: Animals, Apoptosis, Base Sequence, Brain Ischemia, carbon, Caspase 3, Caspase Inhibitors, Cell Line, Cells, Cultured, Electron, Endothelin-1, Female, Genetic Therapy, I2CT, Inbred C57BL, Mice, Microscopy, Nanomedicine, Nanotubes, Neurons, Psychomotor Performance, Rats, RNA, RNA Interference, Small Interfering, Sprague-Dawley, Team-Bianco, Transmission
@article{al-jamal_functional_2011,
title = {Functional motor recovery from brain ischemic insult by carbon nanotube-mediated siRNA silencing},
author = {Khuloud T Al-Jamal and Lisa Gherardini and Giuseppe Bardi and Antonio Nunes and Chang Guo and Cyrill Bussy and Antonia M Herrero and Alberto Bianco and Maurizio Prato and Kostas Kostarelos and Tommaso Pizzorusso},
doi = {10.1073/pnas.1100930108},
issn = {1091-6490},
year = {2011},
date = {2011-07-01},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {108},
number = {27},
pages = {10952--10957},
abstract = {Stroke is the second cause of death worldwide with ischemic stroke accounting for 80% of all stroke insults. Caspase-3 activation contributes to brain tissue loss and downstream biochemical events that lead to programmed cell death after traumatic brain injury. Alleviation of symptoms following ischemic neuronal injury can be potentially achieved by either genetic disruption or pharmacological inhibition of caspases. Here, we studied whether silencing of Caspase-3 using carbon nanotube-mediated in vivo RNA interference (RNAi) could offer a therapeutic opportunity against stroke. Effective delivery of siRNA directly to the CNS has been shown to normalize phenotypes in animal models of several neurological diseases. It is shown here that peri-lesional stereotactic administration of a Caspase-3 siRNA (siCas 3) delivered by functionalized carbon nanotubes (f-CNT) reduced neurodegeneration and promoted functional preservation before and after focal ischemic damage of the rodent motor cortex using an endothelin-1 induced stroke model. These observations illustrate the opportunity offered by carbon nanotube-mediated siRNA delivery and gene silencing of neuronal tissue applicable to a variety of different neuropathological conditions where intervention at well localized brain foci may offer therapeutic and functional benefits.},
keywords = {Animals, Apoptosis, Base Sequence, Brain Ischemia, carbon, Caspase 3, Caspase Inhibitors, Cell Line, Cells, Cultured, Electron, Endothelin-1, Female, Genetic Therapy, I2CT, Inbred C57BL, Mice, Microscopy, Nanomedicine, Nanotubes, Neurons, Psychomotor Performance, Rats, RNA, RNA Interference, Small Interfering, Sprague-Dawley, Team-Bianco, Transmission},
pubstate = {published},
tppubtype = {article}
}
Stroke is the second cause of death worldwide with ischemic stroke accounting for 80% of all stroke insults. Caspase-3 activation contributes to brain tissue loss and downstream biochemical events that lead to programmed cell death after traumatic brain injury. Alleviation of symptoms following ischemic neuronal injury can be potentially achieved by either genetic disruption or pharmacological inhibition of caspases. Here, we studied whether silencing of Caspase-3 using carbon nanotube-mediated in vivo RNA interference (RNAi) could offer a therapeutic opportunity against stroke. Effective delivery of siRNA directly to the CNS has been shown to normalize phenotypes in animal models of several neurological diseases. It is shown here that peri-lesional stereotactic administration of a Caspase-3 siRNA (siCas 3) delivered by functionalized carbon nanotubes (f-CNT) reduced neurodegeneration and promoted functional preservation before and after focal ischemic damage of the rodent motor cortex using an endothelin-1 induced stroke model. These observations illustrate the opportunity offered by carbon nanotube-mediated siRNA delivery and gene silencing of neuronal tissue applicable to a variety of different neuropathological conditions where intervention at well localized brain foci may offer therapeutic and functional benefits.
Murphy Fiona A, Poland Craig A, Duffin Rodger, Al-Jamal Khuloud T, Ali-Boucetta Hanene, Nunes Antonio, Byrne Fiona, Prina-Mello Adriele, Volkov Yuri, Li Shouping, Mather Stephen J, Bianco Alberto, Prato Maurizio, Macnee William, Wallace William A, Kostarelos Kostas, Donaldson Ken
Length-dependent retention of carbon nanotubes in the pleural space of mice initiates sustained inflammation and progressive fibrosis on the parietal pleura Article de journal
Dans: The American Journal of Pathology, vol. 178, no. 6, p. 2587–2600, 2011, ISSN: 1525-2191.
Résumé | Liens | BibTeX | Étiquettes: Animals, carbon, Cell Proliferation, Disease Progression, Emission-Computed, Epithelium, Fibrosis, I2CT, inflammation, Lymph Nodes, Mediastinum, Mice, Nanotubes, Nanowires, Particle Size, Pleura, Pleural Cavity, Single-Photon, Team-Bianco, Time Factors, Tomography, X-Ray Computed
@article{murphy_length-dependent_2011,
title = {Length-dependent retention of carbon nanotubes in the pleural space of mice initiates sustained inflammation and progressive fibrosis on the parietal pleura},
author = {Fiona A Murphy and Craig A Poland and Rodger Duffin and Khuloud T Al-Jamal and Hanene Ali-Boucetta and Antonio Nunes and Fiona Byrne and Adriele Prina-Mello and Yuri Volkov and Shouping Li and Stephen J Mather and Alberto Bianco and Maurizio Prato and William Macnee and William A Wallace and Kostas Kostarelos and Ken Donaldson},
doi = {10.1016/j.ajpath.2011.02.040},
issn = {1525-2191},
year = {2011},
date = {2011-06-01},
journal = {The American Journal of Pathology},
volume = {178},
number = {6},
pages = {2587--2600},
abstract = {The fibrous shape of carbon nanotubes (CNTs) raises concern that they may pose an asbestos-like inhalation hazard, leading to the development of diseases, especially mesothelioma. Direct instillation of long and short CNTs into the pleural cavity, the site of mesothelioma development, produced asbestos-like length-dependent responses. The response to long CNTs and long asbestos was characterized by acute inflammation, leading to progressive fibrosis on the parietal pleura, where stomata of strictly defined size limit the egress of long, but not short, fibers. This was confirmed by demonstrating clearance of short, but not long, CNT and nickel nanowires and by visualizing the migration of short CNTs from the pleural space by single-photon emission computed tomographic imaging. Our data confirm the hypothesis that, although a proportion of all deposited particles passes through the pleura, the pathogenicity of long CNTs and other fibers arises as a result of length-dependent retention at the stomata on the parietal pleura.},
keywords = {Animals, carbon, Cell Proliferation, Disease Progression, Emission-Computed, Epithelium, Fibrosis, I2CT, inflammation, Lymph Nodes, Mediastinum, Mice, Nanotubes, Nanowires, Particle Size, Pleura, Pleural Cavity, Single-Photon, Team-Bianco, Time Factors, Tomography, X-Ray Computed},
pubstate = {published},
tppubtype = {article}
}
The fibrous shape of carbon nanotubes (CNTs) raises concern that they may pose an asbestos-like inhalation hazard, leading to the development of diseases, especially mesothelioma. Direct instillation of long and short CNTs into the pleural cavity, the site of mesothelioma development, produced asbestos-like length-dependent responses. The response to long CNTs and long asbestos was characterized by acute inflammation, leading to progressive fibrosis on the parietal pleura, where stomata of strictly defined size limit the egress of long, but not short, fibers. This was confirmed by demonstrating clearance of short, but not long, CNT and nickel nanowires and by visualizing the migration of short CNTs from the pleural space by single-photon emission computed tomographic imaging. Our data confirm the hypothesis that, although a proportion of all deposited particles passes through the pleura, the pathogenicity of long CNTs and other fibers arises as a result of length-dependent retention at the stomata on the parietal pleura.
Al-Jamal Khuloud T, Nerl Hannah, Müller Karin H, Ali-Boucetta Hanene, Li Shouping, Haynes Peter D, Jinschek Joerg R, Prato Maurizio, Bianco Alberto, Kostarelos Kostas, Porter Alexandra E
Cellular uptake mechanisms of functionalised multi-walled carbon nanotubes by 3D electron tomography imaging Article de journal
Dans: Nanoscale, vol. 3, no. 6, p. 2627–2635, 2011, ISSN: 2040-3372.
Résumé | Liens | BibTeX | Étiquettes: carbon, Cell Line, Cell Membrane, Cytoplasm, Electron Microscope Tomography, Humans, I2CT, imaging, Macrophages, Nanotubes, Phagocytosis, Phagosomes, Team-Bianco, Three-Dimensional, tumor
@article{al-jamal_cellular_2011,
title = {Cellular uptake mechanisms of functionalised multi-walled carbon nanotubes by 3D electron tomography imaging},
author = {Khuloud T Al-Jamal and Hannah Nerl and Karin H Müller and Hanene Ali-Boucetta and Shouping Li and Peter D Haynes and Joerg R Jinschek and Maurizio Prato and Alberto Bianco and Kostas Kostarelos and Alexandra E Porter},
doi = {10.1039/c1nr10080g},
issn = {2040-3372},
year = {2011},
date = {2011-06-01},
journal = {Nanoscale},
volume = {3},
number = {6},
pages = {2627--2635},
abstract = {Carbon nanotubes (CNTs) are being investigated for a variety of biomedical applications. Despite numerous studies, the pathways by which carbon nanotubes enter cells and their subsequent intracellular trafficking and distribution remain poorly determined. Here, we use 3-D electron tomography techniques that offer optimum enhancement of contrast between carbon nanotubes and the plasma membrane to investigate the mechanisms involved in the cellular uptake of shortened, functionalised multi-walled carbon nanotubes (MWNT-NH(3)(+)). Both human lung epithelial (A549) cells, that are almost incapable of phagocytosis and primary macrophages, capable of extremely efficient phagocytosis, were used. We observed that MWNT-NH(3)(+) were internalised in both phagocytic and non-phagocytic cells by any one of three mechanisms: (a) individually via membrane wrapping; (b) individually by direct membrane translocation; and (c) in clusters within vesicular compartments. At early time points following intracellular translocation, we noticed accumulation of nanotube material within various intracellular compartments, while a long-term (14-day) study using primary human macrophages revealed that MWNT-NH(3)(+) were able to escape vesicular (phagosome) entrapment by translocating directly into the cytoplasm.},
keywords = {carbon, Cell Line, Cell Membrane, Cytoplasm, Electron Microscope Tomography, Humans, I2CT, imaging, Macrophages, Nanotubes, Phagocytosis, Phagosomes, Team-Bianco, Three-Dimensional, tumor},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes (CNTs) are being investigated for a variety of biomedical applications. Despite numerous studies, the pathways by which carbon nanotubes enter cells and their subsequent intracellular trafficking and distribution remain poorly determined. Here, we use 3-D electron tomography techniques that offer optimum enhancement of contrast between carbon nanotubes and the plasma membrane to investigate the mechanisms involved in the cellular uptake of shortened, functionalised multi-walled carbon nanotubes (MWNT-NH(3)(+)). Both human lung epithelial (A549) cells, that are almost incapable of phagocytosis and primary macrophages, capable of extremely efficient phagocytosis, were used. We observed that MWNT-NH(3)(+) were internalised in both phagocytic and non-phagocytic cells by any one of three mechanisms: (a) individually via membrane wrapping; (b) individually by direct membrane translocation; and (c) in clusters within vesicular compartments. At early time points following intracellular translocation, we noticed accumulation of nanotube material within various intracellular compartments, while a long-term (14-day) study using primary human macrophages revealed that MWNT-NH(3)(+) were able to escape vesicular (phagosome) entrapment by translocating directly into the cytoplasm.
Singh Prabhpreet, Toma Francesca Maria, Kumar Jitendra, Venkatesh V, Raya Jesus, Prato Maurizio, Verma Sandeep, Bianco Alberto
Carbon nanotube-nucleobase hybrids: nanorings from uracil-modified single-walled carbon nanotubes Article de journal
Dans: Chemistry (Weinheim an Der Bergstrasse, Germany), vol. 17, no. 24, p. 6772–6780, 2011, ISSN: 1521-3765.
Résumé | Liens | BibTeX | Étiquettes: carbon, I2CT, Magnetic Resonance Spectroscopy, Molecular Structure, Nanotubes, Team-Bianco, Uracil
@article{singh_carbon_2011,
title = {Carbon nanotube-nucleobase hybrids: nanorings from uracil-modified single-walled carbon nanotubes},
author = {Prabhpreet Singh and Francesca Maria Toma and Jitendra Kumar and V Venkatesh and Jesus Raya and Maurizio Prato and Sandeep Verma and Alberto Bianco},
doi = {10.1002/chem.201100312},
issn = {1521-3765},
year = {2011},
date = {2011-06-01},
journal = {Chemistry (Weinheim an Der Bergstrasse, Germany)},
volume = {17},
number = {24},
pages = {6772--6780},
abstract = {Single-walled carbon nanotubes (SWCNTs) have been covalently functionalized with uracil nucleobase. The hybrids have been characterized by using complementary spectroscopic and microscopic techniques including solid-state NMR spectroscopy. The uracil-functionalized SWCNTs are able to self-assemble into regular nanorings with a diameter of 50-70 nm, as observed by AFM and TEM. AFM shows that the rings do not have a consistent height and thickness, which indicates that they may be formed by separate bundles of CNTs. The simplest model for the nanoring formation likely involves two bundles of CNTs interacting with each other via uracil-uracil base-pairing at both CNT ends. These nanorings can be envisaged for the development of advanced electronic circuits.},
keywords = {carbon, I2CT, Magnetic Resonance Spectroscopy, Molecular Structure, Nanotubes, Team-Bianco, Uracil},
pubstate = {published},
tppubtype = {article}
}
Single-walled carbon nanotubes (SWCNTs) have been covalently functionalized with uracil nucleobase. The hybrids have been characterized by using complementary spectroscopic and microscopic techniques including solid-state NMR spectroscopy. The uracil-functionalized SWCNTs are able to self-assemble into regular nanorings with a diameter of 50-70 nm, as observed by AFM and TEM. AFM shows that the rings do not have a consistent height and thickness, which indicates that they may be formed by separate bundles of CNTs. The simplest model for the nanoring formation likely involves two bundles of CNTs interacting with each other via uracil-uracil base-pairing at both CNT ends. These nanorings can be envisaged for the development of advanced electronic circuits.
Russier Julie, Ménard-Moyon Cécilia, Venturelli Enrica, Gravel Edmond, Marcolongo Gabriele, Meneghetti Moreno, Doris Eric, Bianco Alberto
Oxidative biodegradation of single- and multi-walled carbon nanotubes Article de journal
Dans: Nanoscale, vol. 3, no. 3, p. 893–896, 2011, ISSN: 2040-3372.
Résumé | Liens | BibTeX | Étiquettes: Absorbable Implants, Biocompatible Materials, Body Fluids, carbon, Horseradish Peroxidase, Hydrogen Peroxide, I2CT, Macromolecular Substances, Materials Testing, Molecular Conformation, Nanotubes, Oxidation-Reduction, Particle Size, Surface Properties, Team-Bianco
@article{russier_oxidative_2011,
title = {Oxidative biodegradation of single- and multi-walled carbon nanotubes},
author = {Julie Russier and Cécilia Ménard-Moyon and Enrica Venturelli and Edmond Gravel and Gabriele Marcolongo and Moreno Meneghetti and Eric Doris and Alberto Bianco},
doi = {10.1039/c0nr00779j},
issn = {2040-3372},
year = {2011},
date = {2011-03-01},
journal = {Nanoscale},
volume = {3},
number = {3},
pages = {893--896},
abstract = {In this study we compare the biodegradation of both single-walled (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) using two different oxidative conditions. In particular, we demonstrate that oxidized multi-walled carbon nanotubes are highly degraded, although not to completeness when treated with horseradish peroxidase (HRP) in the presence of hydrogen peroxide.},
keywords = {Absorbable Implants, Biocompatible Materials, Body Fluids, carbon, Horseradish Peroxidase, Hydrogen Peroxide, I2CT, Macromolecular Substances, Materials Testing, Molecular Conformation, Nanotubes, Oxidation-Reduction, Particle Size, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
In this study we compare the biodegradation of both single-walled (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) using two different oxidative conditions. In particular, we demonstrate that oxidized multi-walled carbon nanotubes are highly degraded, although not to completeness when treated with horseradish peroxidase (HRP) in the presence of hydrogen peroxide.
Ménard-Moyon Cécilia, Fabbro Chiara, Prato Maurizio, Bianco Alberto
One-pot triple functionalization of carbon nanotubes Article de journal
Dans: Chemistry (Weinheim an Der Bergstrasse, Germany), vol. 17, no. 11, p. 3222–3227, 2011, ISSN: 1521-3765.
Résumé | Liens | BibTeX | Étiquettes: Aniline Compounds, Azo Compounds, Benzylamines, carbon, I2CT, Nanotubes, Raman, Spectrophotometry, Spectrum Analysis, Surface Properties, Team-Bianco
@article{menard-moyon_one-pot_2011,
title = {One-pot triple functionalization of carbon nanotubes},
author = {Cécilia Ménard-Moyon and Chiara Fabbro and Maurizio Prato and Alberto Bianco},
doi = {10.1002/chem.201003050},
issn = {1521-3765},
year = {2011},
date = {2011-03-01},
journal = {Chemistry (Weinheim an Der Bergstrasse, Germany)},
volume = {17},
number = {11},
pages = {3222--3227},
abstract = {Carbon nanotubes (CNTs) are very promising as carriers for the delivery of bioactive molecules. The multifunctionalization of CNTs is necessary to impart multimodalities for the development of future CNT-based multipotent therapeutic constructs. In this context, we report the first example of covalent trifunctionalization of different types of CNTs. Our strategy is a simple and efficient methodology based on the simultaneous functionalization of the nanotube surface with three different active groups. The reaction is performed in one step by arylation with diazonium salts generated in situ. The CNTs are functionalized with benzylamine moieties blocked with three different protecting groups that can be selectively removed under specific conditions. The trifunctionalized CNTs were characterized by TEM, thermogravimetric analysis, and Raman and UV/Vis/NIR spectroscopy, while the amine loading was determined by using the Kaiser test. The sequential removal of the protecting groups of the amine functions allows the grafting of the molecules of interest on the nanotube surface to be controlled.},
keywords = {Aniline Compounds, Azo Compounds, Benzylamines, carbon, I2CT, Nanotubes, Raman, Spectrophotometry, Spectrum Analysis, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes (CNTs) are very promising as carriers for the delivery of bioactive molecules. The multifunctionalization of CNTs is necessary to impart multimodalities for the development of future CNT-based multipotent therapeutic constructs. In this context, we report the first example of covalent trifunctionalization of different types of CNTs. Our strategy is a simple and efficient methodology based on the simultaneous functionalization of the nanotube surface with three different active groups. The reaction is performed in one step by arylation with diazonium salts generated in situ. The CNTs are functionalized with benzylamine moieties blocked with three different protecting groups that can be selectively removed under specific conditions. The trifunctionalized CNTs were characterized by TEM, thermogravimetric analysis, and Raman and UV/Vis/NIR spectroscopy, while the amine loading was determined by using the Kaiser test. The sequential removal of the protecting groups of the amine functions allows the grafting of the molecules of interest on the nanotube surface to be controlled.
Gaillard Claire, Duval Monique, Dumortier Hélène, Bianco Alberto
Carbon nanotube-coupled cell adhesion peptides are non-immunogenic: a promising step toward new biomedical devices Article de journal
Dans: Journal of Peptide Science: An Official Publication of the European Peptide Society, vol. 17, no. 2, p. 139–142, 2011, ISSN: 1099-1387.
Résumé | Liens | BibTeX | Étiquettes: carbon, Dumortier, Enzyme-Linked Immunosorbent Assay, I2CT, Nanotubes, Peptides, Team-Bianco, Team-Dumortier
@article{gaillard_carbon_2011,
title = {Carbon nanotube-coupled cell adhesion peptides are non-immunogenic: a promising step toward new biomedical devices},
author = {Claire Gaillard and Monique Duval and Hélène Dumortier and Alberto Bianco},
doi = {10.1002/psc.1290},
issn = {1099-1387},
year = {2011},
date = {2011-02-01},
journal = {Journal of Peptide Science: An Official Publication of the European Peptide Society},
volume = {17},
number = {2},
pages = {139--142},
abstract = {Carbon nanotubes functionalized with cell adhesion peptides can be considered as novel, promising candidates for the development of advanced drug delivery systems or for designing new generation of self-assembling nerve 'bridges'. An important step toward the integration of these types of conjugates in living bodies is the assessment of their impact on the immune system. In this direction, an integrin-derived peptide has been covalently conjugated to carbon nanotubes. Following intraperitoneal administration, peptide-carbon nanotubes do not trigger an anti-peptide antibody production. Demonstration of the immune neutrality of peptide-carbon nanotubes reinforces their potential use as substrates for neuronal regeneration in vivo.},
keywords = {carbon, Dumortier, Enzyme-Linked Immunosorbent Assay, I2CT, Nanotubes, Peptides, Team-Bianco, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes functionalized with cell adhesion peptides can be considered as novel, promising candidates for the development of advanced drug delivery systems or for designing new generation of self-assembling nerve 'bridges'. An important step toward the integration of these types of conjugates in living bodies is the assessment of their impact on the immune system. In this direction, an integrin-derived peptide has been covalently conjugated to carbon nanotubes. Following intraperitoneal administration, peptide-carbon nanotubes do not trigger an anti-peptide antibody production. Demonstration of the immune neutrality of peptide-carbon nanotubes reinforces their potential use as substrates for neuronal regeneration in vivo.
Serag Maged F, Kaji Noritada, Gaillard Claire, Okamoto Yukihiro, Terasaka Kazuyoshi, Jabasini Mohammad, Tokeshi Manabu, Mizukami Hajime, Bianco Alberto, Baba Yoshinobu
Trafficking and subcellular localization of multiwalled carbon nanotubes in plant cells Article de journal
Dans: ACS nano, vol. 5, no. 1, p. 493–499, 2011, ISSN: 1936-086X.
Résumé | Liens | BibTeX | Étiquettes: Biological Transport, carbon, Catharanthus, Cell Membrane, Endosomes, I2CT, Intracellular Space, Nanotubes, Protoplasts, Team-Bianco
@article{serag_trafficking_2011,
title = {Trafficking and subcellular localization of multiwalled carbon nanotubes in plant cells},
author = {Maged F Serag and Noritada Kaji and Claire Gaillard and Yukihiro Okamoto and Kazuyoshi Terasaka and Mohammad Jabasini and Manabu Tokeshi and Hajime Mizukami and Alberto Bianco and Yoshinobu Baba},
doi = {10.1021/nn102344t},
issn = {1936-086X},
year = {2011},
date = {2011-01-01},
journal = {ACS nano},
volume = {5},
number = {1},
pages = {493--499},
abstract = {Major barriers to delivery of biomolecules are crossing the cellular membranes and achieving a high cytoplasmic concentration by circumventing entrapment into endosomes and other lytic organelles. Motivated by such aim, we have investigated the capability of multiwalled carbon nanotubes (MWCNTs) to penetrate the cell membrane of plant protoplasts (plant cells made devoid of their cell walls via enzymatic treatment) and studied their internalization mechanism via confocal imaging and TEM techniques. Our results indentified an endosome-escaping uptake mode of MWCNTs by plant protoplasts. Moreover, short MWCNTs (textbackslashtextless100 nm) were observed to target specific cellular substructures including the nucleus, plastids, and vacuoles. These findings are expected to have a significant impact on plant cell biology and transformation technologies.},
keywords = {Biological Transport, carbon, Catharanthus, Cell Membrane, Endosomes, I2CT, Intracellular Space, Nanotubes, Protoplasts, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Major barriers to delivery of biomolecules are crossing the cellular membranes and achieving a high cytoplasmic concentration by circumventing entrapment into endosomes and other lytic organelles. Motivated by such aim, we have investigated the capability of multiwalled carbon nanotubes (MWCNTs) to penetrate the cell membrane of plant protoplasts (plant cells made devoid of their cell walls via enzymatic treatment) and studied their internalization mechanism via confocal imaging and TEM techniques. Our results indentified an endosome-escaping uptake mode of MWCNTs by plant protoplasts. Moreover, short MWCNTs (textbackslashtextless100 nm) were observed to target specific cellular substructures including the nucleus, plastids, and vacuoles. These findings are expected to have a significant impact on plant cell biology and transformation technologies.
Benincasa Monica, Pacor Sabrina, Wu Wei, Prato Maurizio, Bianco Alberto, Gennaro Renato
Antifungal activity of amphotericin B conjugated to carbon nanotubes Article de journal
Dans: ACS nano, vol. 5, no. 1, p. 199–208, 2011, ISSN: 1936-086X.
Résumé | Liens | BibTeX | Étiquettes: Amphotericin B, Antifungal Agents, Candida, carbon, Cell Membrane, Deoxycholic Acid, Drug Design, Drug Resistance, Fungal, Humans, I2CT, Jurkat Cells, Kinetics, Membrane Potentials, Nanotubes, Team-Bianco
@article{benincasa_antifungal_2011,
title = {Antifungal activity of amphotericin B conjugated to carbon nanotubes},
author = {Monica Benincasa and Sabrina Pacor and Wei Wu and Maurizio Prato and Alberto Bianco and Renato Gennaro},
doi = {10.1021/nn1023522},
issn = {1936-086X},
year = {2011},
date = {2011-01-01},
journal = {ACS nano},
volume = {5},
number = {1},
pages = {199--208},
abstract = {Amphotericin B (AMB) has long been considered the most effective drug in the treatment of serious invasive fungal infections. There are, however, major limitations to its use, due to several adverse effects, including acute infusional reactions and, most relevant, a dose-dependent nephrotoxicity. At least some of these effects are attributed to the aggregation of AMB as a result of its poor water solubility. To overcome this problem, reformulated versions of the drug have been developed, including a micellar dispersion of AMB with sodium deoxycholate (AMBD), its encapsulation into liposomes, or its incorporation into lipidic complexes. The development of nanobiotechnologies provides novel potential drug delivery systems that make use of nanomaterials such as functionalized carbon nanotubes (f-CNTs), which are emerging as an innovative and efficient tool for the transport and cellular translocation of therapeutic molecules. In this study, we prepared two conjugates between f-CNTs and AMB. The antifungal activity of these conjugates was tested against a collection of reference and clinical fungal strains, in comparison to that of AMB alone or AMBD. Measured minimum inhibition concentration (MIC) values for f-CNT-AMB conjugates were either comparable to or better than those displayed by AMB and AMBD. Furthermore, AMBD-resistant Candida strains were found to be susceptible to f-CNT-AMB 1. Additional studies, aimed at understanding the mechanism of action of the conjugates, suggest a nonlytic mechanism, since the compounds show a major permeabilizing effect on the tested fungal strains only after extended incubation. Interestingly, the f-CNT-AMB 1 does not show any significant toxic effect on Jurkat cells at antifungal concentrations.},
keywords = {Amphotericin B, Antifungal Agents, Candida, carbon, Cell Membrane, Deoxycholic Acid, Drug Design, Drug Resistance, Fungal, Humans, I2CT, Jurkat Cells, Kinetics, Membrane Potentials, Nanotubes, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Amphotericin B (AMB) has long been considered the most effective drug in the treatment of serious invasive fungal infections. There are, however, major limitations to its use, due to several adverse effects, including acute infusional reactions and, most relevant, a dose-dependent nephrotoxicity. At least some of these effects are attributed to the aggregation of AMB as a result of its poor water solubility. To overcome this problem, reformulated versions of the drug have been developed, including a micellar dispersion of AMB with sodium deoxycholate (AMBD), its encapsulation into liposomes, or its incorporation into lipidic complexes. The development of nanobiotechnologies provides novel potential drug delivery systems that make use of nanomaterials such as functionalized carbon nanotubes (f-CNTs), which are emerging as an innovative and efficient tool for the transport and cellular translocation of therapeutic molecules. In this study, we prepared two conjugates between f-CNTs and AMB. The antifungal activity of these conjugates was tested against a collection of reference and clinical fungal strains, in comparison to that of AMB alone or AMBD. Measured minimum inhibition concentration (MIC) values for f-CNT-AMB conjugates were either comparable to or better than those displayed by AMB and AMBD. Furthermore, AMBD-resistant Candida strains were found to be susceptible to f-CNT-AMB 1. Additional studies, aimed at understanding the mechanism of action of the conjugates, suggest a nonlytic mechanism, since the compounds show a major permeabilizing effect on the tested fungal strains only after extended incubation. Interestingly, the f-CNT-AMB 1 does not show any significant toxic effect on Jurkat cells at antifungal concentrations.
2010
Cellot Giada, Ballerini Laura, Prato Maurizio, Bianco Alberto
Neurons are able to internalize soluble carbon nanotubes: new opportunities or old risks? Article de journal
Dans: Small (Weinheim an Der Bergstrasse, Germany), vol. 6, no. 23, p. 2630–2633, 2010, ISSN: 1613-6829.
Liens | BibTeX | Étiquettes: carbon, Cell Line, Cells, Cultured, Humans, I2CT, Nanotubes, Neurons, Team-Bianco, tumor
@article{cellot_neurons_2010,
title = {Neurons are able to internalize soluble carbon nanotubes: new opportunities or old risks?},
author = {Giada Cellot and Laura Ballerini and Maurizio Prato and Alberto Bianco},
doi = {10.1002/smll.201000906},
issn = {1613-6829},
year = {2010},
date = {2010-12-01},
journal = {Small (Weinheim an Der Bergstrasse, Germany)},
volume = {6},
number = {23},
pages = {2630--2633},
keywords = {carbon, Cell Line, Cells, Cultured, Humans, I2CT, Nanotubes, Neurons, Team-Bianco, tumor},
pubstate = {published},
tppubtype = {article}
}
Al-Jamal Khuloud T, Toma Francesca M, Yilmazer Açelya, Ali-Boucetta Hanene, Nunes Antonio, Herrero Maria-Antonia, Tian Bowen, Eddaoudi Ayad, Eddaoui Ayad, Al-Jamal Wafa' T, Bianco Alberto, Prato Maurizio, Kostarelo Kostas
Enhanced cellular internalization and gene silencing with a series of cationic dendron-multiwalled carbon nanotube:siRNA complexes Article de journal
Dans: FASEB journal: official publication of the Federation of American Societies for Experimental Biology, vol. 24, no. 11, p. 4354–4365, 2010, ISSN: 1530-6860.
Résumé | Liens | BibTeX | Étiquettes: Biological Transport, carbon, Cations, Cell Line, Cell Survival, Gene Silencing, HeLa Cells, Humans, I2CT, Models, Molecular, Nanotubes, RNA, Small Interfering, Team-Bianco, Transfection, tumor
@article{al-jamal_enhanced_2010,
title = {Enhanced cellular internalization and gene silencing with a series of cationic dendron-multiwalled carbon nanotube:siRNA complexes},
author = {Khuloud T Al-Jamal and Francesca M Toma and Açelya Yilmazer and Hanene Ali-Boucetta and Antonio Nunes and Maria-Antonia Herrero and Bowen Tian and Ayad Eddaoudi and Ayad Eddaoui and Wafa' T Al-Jamal and Alberto Bianco and Maurizio Prato and Kostas Kostarelo},
doi = {10.1096/fj.09-141036},
issn = {1530-6860},
year = {2010},
date = {2010-11-01},
journal = {FASEB journal: official publication of the Federation of American Societies for Experimental Biology},
volume = {24},
number = {11},
pages = {4354--4365},
abstract = {One of the major obstacles to the clinical development of gene silencing by small interfering RNA (siRNA) is its effective cytoplasmic delivery. Carbon nanotubes have been proposed as novel nanomaterials that can offer significant advantages for the intracellular delivery of nucleic acids, such as siRNA. We recently demonstrated in a proof-of-principle study that amino-functionalized multiwalled carbon nanotubes (f-MWNT) can effectively deliver in vivo an siRNA sequence, triggering cell apoptosis that results in human lung xenograft eradication and prolonged survival. In the present study, we demonstrate how a newly synthesized series of polycationic dendron-MWNT constructs with a precisely tailored number of amino functions (dendron generations) can complex and effectively deliver double-stranded siRNA to achieve gene silencing in vitro. A systematic comparison between the f-MWNT series in terms of cellular uptake, cytotoxicity, and siRNA complexation is offered. Significant improvement in siRNA delivery with the dendron-MWNT conjugates is shown, and gene silencing was obtained in 2 human cell lines using 2 different siRNA sequences. The study reveals that through f-MWNT structure-biological function analysis novel nanotube-based siRNA transfer vectors can be designed with minimal cytotoxicity and effective delivery and gene-silencing capabilities.},
keywords = {Biological Transport, carbon, Cations, Cell Line, Cell Survival, Gene Silencing, HeLa Cells, Humans, I2CT, Models, Molecular, Nanotubes, RNA, Small Interfering, Team-Bianco, Transfection, tumor},
pubstate = {published},
tppubtype = {article}
}
One of the major obstacles to the clinical development of gene silencing by small interfering RNA (siRNA) is its effective cytoplasmic delivery. Carbon nanotubes have been proposed as novel nanomaterials that can offer significant advantages for the intracellular delivery of nucleic acids, such as siRNA. We recently demonstrated in a proof-of-principle study that amino-functionalized multiwalled carbon nanotubes (f-MWNT) can effectively deliver in vivo an siRNA sequence, triggering cell apoptosis that results in human lung xenograft eradication and prolonged survival. In the present study, we demonstrate how a newly synthesized series of polycationic dendron-MWNT constructs with a precisely tailored number of amino functions (dendron generations) can complex and effectively deliver double-stranded siRNA to achieve gene silencing in vitro. A systematic comparison between the f-MWNT series in terms of cellular uptake, cytotoxicity, and siRNA complexation is offered. Significant improvement in siRNA delivery with the dendron-MWNT conjugates is shown, and gene silencing was obtained in 2 human cell lines using 2 different siRNA sequences. The study reveals that through f-MWNT structure-biological function analysis novel nanotube-based siRNA transfer vectors can be designed with minimal cytotoxicity and effective delivery and gene-silencing capabilities.
den Bossche Jeroen Van, Al-Jamal Wafa' T, Tian Bowen, Nunes Antonio, Fabbro Chiara, Bianco Alberto, Prato Maurizio, Kostarelos Kostas
Efficient receptor-independent intracellular translocation of aptamers mediated by conjugation to carbon nanotubes Article de journal
Dans: Chemical Communications (Cambridge, England), vol. 46, no. 39, p. 7379–7381, 2010, ISSN: 1364-548X.
Résumé | Liens | BibTeX | Étiquettes: Aptamers, Base Sequence, Biological Transport, carbon, Cell Line, Cell Surface, DNA Primers, Electron, Electrophoresis, Humans, I2CT, Microscopy, Nanotubes, Nucleotide, Polyacrylamide Gel, Receptors, Team-Bianco, Transmission, tumor
@article{van_den_bossche_efficient_2010,
title = {Efficient receptor-independent intracellular translocation of aptamers mediated by conjugation to carbon nanotubes},
author = {Jeroen Van den Bossche and Wafa' T Al-Jamal and Bowen Tian and Antonio Nunes and Chiara Fabbro and Alberto Bianco and Maurizio Prato and Kostas Kostarelos},
doi = {10.1039/c0cc02092c},
issn = {1364-548X},
year = {2010},
date = {2010-10-01},
journal = {Chemical Communications (Cambridge, England)},
volume = {46},
number = {39},
pages = {7379--7381},
abstract = {We have covalently grafted aptamers onto carboxylated carbon nanotubes to design a novel vector system that can easily translocate into the cytosol of different cell types independent of receptor-mediated uptake. We propose the use of carbon nanotubes for the efficient intracellular delivery of biologically active aptamers for potential therapeutic applications.},
keywords = {Aptamers, Base Sequence, Biological Transport, carbon, Cell Line, Cell Surface, DNA Primers, Electron, Electrophoresis, Humans, I2CT, Microscopy, Nanotubes, Nucleotide, Polyacrylamide Gel, Receptors, Team-Bianco, Transmission, tumor},
pubstate = {published},
tppubtype = {article}
}
We have covalently grafted aptamers onto carboxylated carbon nanotubes to design a novel vector system that can easily translocate into the cytosol of different cell types independent of receptor-mediated uptake. We propose the use of carbon nanotubes for the efficient intracellular delivery of biologically active aptamers for potential therapeutic applications.
Marega Riccardo, Aroulmoji Vincent, Bergamin Massimo, Feruglio Luigi, Dinon Francesca, Bianco Alberto, Murano Erminio, Prato Maurizio
Two-dimensional diffusion-ordered NMR spectroscopy as a tool for monitoring functionalized carbon nanotube purification and composition Article de journal
Dans: ACS nano, vol. 4, no. 4, p. 2051–2058, 2010, ISSN: 1936-086X.
Résumé | Liens | BibTeX | Étiquettes: carbon, Diffusion, I2CT, Magnetic Resonance Spectroscopy, Nanotubes, Polyethylene Glycols, Solubility, Team-Bianco, Temperature, water
@article{marega_two-dimensional_2010,
title = {Two-dimensional diffusion-ordered NMR spectroscopy as a tool for monitoring functionalized carbon nanotube purification and composition},
author = {Riccardo Marega and Vincent Aroulmoji and Massimo Bergamin and Luigi Feruglio and Francesca Dinon and Alberto Bianco and Erminio Murano and Maurizio Prato},
doi = {10.1021/nn100257h},
issn = {1936-086X},
year = {2010},
date = {2010-04-01},
journal = {ACS nano},
volume = {4},
number = {4},
pages = {2051--2058},
abstract = {Functionalized carbon nanotube (CNT) derivatives are currently under thorough investigation in different biomedical investigations. In this field of research, the composition of sample either in terms of covalently attached or physisorbed moieties can greatly affect the observed results and hamper the comparison between different studies. Therefore, the availability of a fast and reliable analytical technique to assess both the type of interaction (covalent vs noncovalent) and the composition of CNT conjugates is of great importance. Here we describe that the two-dimensional diffusion-ordered (DOSY) NMR spectroscopy is extremely useful to discriminate between conjugated and unconjugated polyethylene glycol groups in samples obtained by condensation with oxidized single-walled carbon nanotubes (SWNTs). This fast and nondestructive technique allows us to follow the removal of unconjugated polyethylene glycol chains during the purification. In particular, DOSY analysis reveal that about 1/3 (wt %) of the polyethylene glycol used for the condensation remained physisorbed to functionalized SWNTs after dialysis. Complete elimination of physisorbed polyethylene glycol was achieved using diafiltration.},
keywords = {carbon, Diffusion, I2CT, Magnetic Resonance Spectroscopy, Nanotubes, Polyethylene Glycols, Solubility, Team-Bianco, Temperature, water},
pubstate = {published},
tppubtype = {article}
}
Functionalized carbon nanotube (CNT) derivatives are currently under thorough investigation in different biomedical investigations. In this field of research, the composition of sample either in terms of covalently attached or physisorbed moieties can greatly affect the observed results and hamper the comparison between different studies. Therefore, the availability of a fast and reliable analytical technique to assess both the type of interaction (covalent vs noncovalent) and the composition of CNT conjugates is of great importance. Here we describe that the two-dimensional diffusion-ordered (DOSY) NMR spectroscopy is extremely useful to discriminate between conjugated and unconjugated polyethylene glycol groups in samples obtained by condensation with oxidized single-walled carbon nanotubes (SWNTs). This fast and nondestructive technique allows us to follow the removal of unconjugated polyethylene glycol chains during the purification. In particular, DOSY analysis reveal that about 1/3 (wt %) of the polyethylene glycol used for the condensation remained physisorbed to functionalized SWNTs after dialysis. Complete elimination of physisorbed polyethylene glycol was achieved using diafiltration.
Samorì Cristian, Ali-Boucetta Hanene, Sainz Raquel, Guo Chang, Toma Francesca Maria, Fabbro Chiara, da Ros Tatiana, Prato Maurizio, Kostarelos Kostas, Bianco Alberto
Enhanced anticancer activity of multi-walled carbon nanotube-methotrexate conjugates using cleavable linkers Article de journal
Dans: Chemical Communications (Cambridge, England), vol. 46, no. 9, p. 1494–1496, 2010, ISSN: 1364-548X.
Résumé | Liens | BibTeX | Étiquettes: Antineoplastic Agents, Azo Compounds, carbon, Cell Line, Cross-Linking Reagents, Humans, I2CT, Methotrexate, Nanotubes, Team-Bianco, Thiosemicarbazones, tumor
@article{samori_enhanced_2010,
title = {Enhanced anticancer activity of multi-walled carbon nanotube-methotrexate conjugates using cleavable linkers},
author = {Cristian Samorì and Hanene Ali-Boucetta and Raquel Sainz and Chang Guo and Francesca Maria Toma and Chiara Fabbro and Tatiana da Ros and Maurizio Prato and Kostas Kostarelos and Alberto Bianco},
doi = {10.1039/b923560d},
issn = {1364-548X},
year = {2010},
date = {2010-03-01},
journal = {Chemical Communications (Cambridge, England)},
volume = {46},
number = {9},
pages = {1494--1496},
abstract = {Methotrexate was tethered to multi-walled carbon nanotubes through different cleavable linkers exploiting the ammonium functionalities introduced by 1,3-dipolar cycloaddition reaction of azomethine ylides to the nanotubes. The new nanobio-hybrid conjugates were internalized into human breast cancer cells and it was shown that the cytotoxic activity was strongly dependent on the presence and type of linker.},
keywords = {Antineoplastic Agents, Azo Compounds, carbon, Cell Line, Cross-Linking Reagents, Humans, I2CT, Methotrexate, Nanotubes, Team-Bianco, Thiosemicarbazones, tumor},
pubstate = {published},
tppubtype = {article}
}
Methotrexate was tethered to multi-walled carbon nanotubes through different cleavable linkers exploiting the ammonium functionalities introduced by 1,3-dipolar cycloaddition reaction of azomethine ylides to the nanotubes. The new nanobio-hybrid conjugates were internalized into human breast cancer cells and it was shown that the cytotoxic activity was strongly dependent on the presence and type of linker.
Ménard-Moyon Cécilia, Kostarelos Kostas, Prato Maurizio, Bianco Alberto
Functionalized carbon nanotubes for probing and modulating molecular functions Article de journal
Dans: Chemistry & Biology, vol. 17, no. 2, p. 107–115, 2010, ISSN: 1879-1301.
Résumé | Liens | BibTeX | Étiquettes: Antibodies, Antigens, Atomic Force, Biosensing Techniques, carbon, Drug Delivery Systems, enzymes, Glycoproteins, I2CT, Ion Channels, Microscopy, Nanotubes, RNA, Small Interfering, Team-Bianco
@article{menard-moyon_functionalized_2010,
title = {Functionalized carbon nanotubes for probing and modulating molecular functions},
author = {Cécilia Ménard-Moyon and Kostas Kostarelos and Maurizio Prato and Alberto Bianco},
doi = {10.1016/j.chembiol.2010.01.009},
issn = {1879-1301},
year = {2010},
date = {2010-02-01},
journal = {Chemistry & Biology},
volume = {17},
number = {2},
pages = {107--115},
abstract = {Carbon nanotubes (CNTs) entered the domain of biological research a few years ago, creating a significant amount of interest due to their extraordinary physicochemical properties. The integration of CNT-based strategies with biology necessitates a multidisciplinary approach that requires competences in the diverse fields of chemistry, physics, and life sciences. In the biomedical domain CNTs are extensively explored as novel drug delivery systems for therapy and diagnosis. Additionally, CNTs can also be designed as new tools for modulation of molecular functions, by directly affecting various biological processes or by interaction with bioactive molecules. The aim of this review is to discuss how CNTs can be exploited as new probes for molecular functions. The different sections illustrate various applications of CNTs, including gene silencing, surface cell interactions via glycoproteins, biosensing, intracellular drug delivery using an atomic force microscopy tip-based nanoinjector, modulation of antibody/antigen interaction and enzyme activity, and blocking of ion channels.},
keywords = {Antibodies, Antigens, Atomic Force, Biosensing Techniques, carbon, Drug Delivery Systems, enzymes, Glycoproteins, I2CT, Ion Channels, Microscopy, Nanotubes, RNA, Small Interfering, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes (CNTs) entered the domain of biological research a few years ago, creating a significant amount of interest due to their extraordinary physicochemical properties. The integration of CNT-based strategies with biology necessitates a multidisciplinary approach that requires competences in the diverse fields of chemistry, physics, and life sciences. In the biomedical domain CNTs are extensively explored as novel drug delivery systems for therapy and diagnosis. Additionally, CNTs can also be designed as new tools for modulation of molecular functions, by directly affecting various biological processes or by interaction with bioactive molecules. The aim of this review is to discuss how CNTs can be exploited as new probes for molecular functions. The different sections illustrate various applications of CNTs, including gene silencing, surface cell interactions via glycoproteins, biosensing, intracellular drug delivery using an atomic force microscopy tip-based nanoinjector, modulation of antibody/antigen interaction and enzyme activity, and blocking of ion channels.
2009
Partidos Charalambos D, Hoebeke Johan, Wieckowski Sébastien, Chaloin Olivier, Bianco Alberto, Moreau Emmanuel, Briand Jean-Paul, Desgranges Claude, Muller Sylviane
Immunomodulatory consequences of ODN CpG-polycation complexes Article de journal
Dans: Methods (San Diego, Calif.), vol. 49, no. 4, p. 328–333, 2009, ISSN: 1095-9130.
Résumé | Liens | BibTeX | Étiquettes: Animals, carbon, CpG Islands, Humans, I2CT, Immunologic Factors, Nanotubes, Oligodeoxyribonucleotides, Polyamines, Team-Bianco, Transcriptional Activation
@article{partidos_immunomodulatory_2009,
title = {Immunomodulatory consequences of ODN CpG-polycation complexes},
author = {Charalambos D Partidos and Johan Hoebeke and Sébastien Wieckowski and Olivier Chaloin and Alberto Bianco and Emmanuel Moreau and Jean-Paul Briand and Claude Desgranges and Sylviane Muller},
doi = {10.1016/j.ymeth.2009.03.005},
issn = {1095-9130},
year = {2009},
date = {2009-12-01},
journal = {Methods (San Diego, Calif.)},
volume = {49},
number = {4},
pages = {328--333},
abstract = {Immunostimulatory ODN CpGs have extensively been tested as adjuvants and immunotherapeutics and hold a lot of promise for human use. In our studies we took advantage of their negative charge to study their biological activities after being complexed with carbon nanotubes, a novel vector for vaccine delivery and Tat protein of HIV, a target protein for therapeutic or prophylactic intervention. In the case of carbon nanotubes, ODN CpGs were able to form stable complexes based on charge interaction and exert increased immunostimulatory activity in vitro. With regard to the Tat protein, ODN CpGs were shown to bind effectively through the basic domain of the protein representing residues 44-61. Moreover, using surface Plasmon Resonance Technology and an in vitro cellular system, ODN CpGs were shown to inhibit the interaction of Tat protein with the transactivation responsive element, a bulged RNA hairpin structure. However, when ODN CpGs were complexed with Tat they readily increased the apoptotic properties of this protein as studied in CD3-stimulated Jurkat cells. Overall, our findings together with published data support the view that for harnessing the beneficial effects of ODN CpGs a careful consideration has to be given depending on the target intervention.},
keywords = {Animals, carbon, CpG Islands, Humans, I2CT, Immunologic Factors, Nanotubes, Oligodeoxyribonucleotides, Polyamines, Team-Bianco, Transcriptional Activation},
pubstate = {published},
tppubtype = {article}
}
Immunostimulatory ODN CpGs have extensively been tested as adjuvants and immunotherapeutics and hold a lot of promise for human use. In our studies we took advantage of their negative charge to study their biological activities after being complexed with carbon nanotubes, a novel vector for vaccine delivery and Tat protein of HIV, a target protein for therapeutic or prophylactic intervention. In the case of carbon nanotubes, ODN CpGs were able to form stable complexes based on charge interaction and exert increased immunostimulatory activity in vitro. With regard to the Tat protein, ODN CpGs were shown to bind effectively through the basic domain of the protein representing residues 44-61. Moreover, using surface Plasmon Resonance Technology and an in vitro cellular system, ODN CpGs were shown to inhibit the interaction of Tat protein with the transactivation responsive element, a bulged RNA hairpin structure. However, when ODN CpGs were complexed with Tat they readily increased the apoptotic properties of this protein as studied in CD3-stimulated Jurkat cells. Overall, our findings together with published data support the view that for harnessing the beneficial effects of ODN CpGs a careful consideration has to be given depending on the target intervention.
Kostarelos K, Bianco A, Prato M
Promises, facts and challenges for carbon nanotubes in imaging and therapeutics Article de journal
Dans: Nature Nanotechnology, vol. 4, no. 10, p. 627–633, 2009, ISSN: 1748-3395.
Résumé | Liens | BibTeX | Étiquettes: Animals, carbon, Diagnostic Imaging, Drug Evaluation, Humans, I2CT, Nanomedicine, Nanotubes, Preclinical, Team-Bianco, therapeutics
@article{kostarelos_promises_2009,
title = {Promises, facts and challenges for carbon nanotubes in imaging and therapeutics},
author = {K Kostarelos and A Bianco and M Prato},
doi = {10.1038/nnano.2009.241},
issn = {1748-3395},
year = {2009},
date = {2009-10-01},
journal = {Nature Nanotechnology},
volume = {4},
number = {10},
pages = {627--633},
abstract = {The use of carbon nanotubes in medicine is now at the crossroads between a proof-of-principle concept and an established preclinical candidate for a variety of therapeutic and diagnostic applications. Progress towards clinical trials will depend on the outcomes of efficacy and toxicology studies, which will provide the necessary risk-to-benefit assessments for carbon-nanotube-based materials. Here we focus on carbon nanotubes that have been studied in preclinical animal models, and draw attention to the promises, facts and challenges of these materials as they transition from research to the clinical phase. We address common questions regarding the use of carbon nanotubes in disease imaging and therapy, and highlight the opportunities and challenges ahead.},
keywords = {Animals, carbon, Diagnostic Imaging, Drug Evaluation, Humans, I2CT, Nanomedicine, Nanotubes, Preclinical, Team-Bianco, therapeutics},
pubstate = {published},
tppubtype = {article}
}
The use of carbon nanotubes in medicine is now at the crossroads between a proof-of-principle concept and an established preclinical candidate for a variety of therapeutic and diagnostic applications. Progress towards clinical trials will depend on the outcomes of efficacy and toxicology studies, which will provide the necessary risk-to-benefit assessments for carbon-nanotube-based materials. Here we focus on carbon nanotubes that have been studied in preclinical animal models, and draw attention to the promises, facts and challenges of these materials as they transition from research to the clinical phase. We address common questions regarding the use of carbon nanotubes in disease imaging and therapy, and highlight the opportunities and challenges ahead.
Singh Prabhpreet, Kumar Jitendra, Toma Francesca Maria, Raya Jesus, Prato Maurizio, Fabre Bruno, Verma Sandeep, Bianco Alberto
Synthesis and characterization of nucleobase-carbon nanotube hybrids Article de journal
Dans: Journal of the American Chemical Society, vol. 131, no. 37, p. 13555–13562, 2009, ISSN: 1520-5126.
Résumé | Liens | BibTeX | Étiquettes: Adenine, Amides, Amines, Biosensing Techniques, carbon, Catalysis, Electrochemistry, Graphite, I2CT, Magnetic Resonance Spectroscopy, Nanotubes, Nanowires, Surface Properties, Team-Bianco
@article{singh_synthesis_2009,
title = {Synthesis and characterization of nucleobase-carbon nanotube hybrids},
author = {Prabhpreet Singh and Jitendra Kumar and Francesca Maria Toma and Jesus Raya and Maurizio Prato and Bruno Fabre and Sandeep Verma and Alberto Bianco},
doi = {10.1021/ja905041b},
issn = {1520-5126},
year = {2009},
date = {2009-09-01},
journal = {Journal of the American Chemical Society},
volume = {131},
number = {37},
pages = {13555--13562},
abstract = {We report the synthesis and characterization of adenine-single-walled carbon nanotube (SWCNT) hybrid materials, where for the first time nucleobases are covalently attached to the exosurface of SWCNTs. The structural properties of all hybrids have been characterized using usual spectroscopic and microscopic techniques. The degree of functional groups for functionalized SWCNTs (f-SWCNTs) 2a and 2b is one adenine group for each 26 and 37 carbon atoms, respectively. Solid-state magic angle spinning (13)C NMR spectroscopy (MAS NMR) and electrochemistry have been also applied for the characterization of these f-SWCNTs. AFM images of f-SWCNT 2b showed an interesting feature of horizontally aligned nanotubes along the surface when deposited on highly oriented pyrolytic graphite surface. Furthermore, we evaluated the coordinating ability of these hybrid materials toward silver ions, and interestingly, we found a pattern of silver nanoparticles localized over the surface of the carbon nanotube network. The presence of aligned and randomly oriented CNTs and their ability to coordinate with metal ions make this class of materials very interesting for applications in the development of novel electronic devices and as new supports for different catalytic transformations.},
keywords = {Adenine, Amides, Amines, Biosensing Techniques, carbon, Catalysis, Electrochemistry, Graphite, I2CT, Magnetic Resonance Spectroscopy, Nanotubes, Nanowires, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
We report the synthesis and characterization of adenine-single-walled carbon nanotube (SWCNT) hybrid materials, where for the first time nucleobases are covalently attached to the exosurface of SWCNTs. The structural properties of all hybrids have been characterized using usual spectroscopic and microscopic techniques. The degree of functional groups for functionalized SWCNTs (f-SWCNTs) 2a and 2b is one adenine group for each 26 and 37 carbon atoms, respectively. Solid-state magic angle spinning (13)C NMR spectroscopy (MAS NMR) and electrochemistry have been also applied for the characterization of these f-SWCNTs. AFM images of f-SWCNT 2b showed an interesting feature of horizontally aligned nanotubes along the surface when deposited on highly oriented pyrolytic graphite surface. Furthermore, we evaluated the coordinating ability of these hybrid materials toward silver ions, and interestingly, we found a pattern of silver nanoparticles localized over the surface of the carbon nanotube network. The presence of aligned and randomly oriented CNTs and their ability to coordinate with metal ions make this class of materials very interesting for applications in the development of novel electronic devices and as new supports for different catalytic transformations.
Singh Prabhpreet, Campidelli Stéphane, Giordani Silvia, Bonifazi Davide, Bianco Alberto, Prato Maurizio
Organic functionalisation and characterisation of single-walled carbon nanotubes Article de journal
Dans: Chemical Society Reviews, vol. 38, no. 8, p. 2214–2230, 2009, ISSN: 1460-4744.
Résumé | Liens | BibTeX | Étiquettes: Alkylation, carbon, Esterification, Free Radicals, Halogenation, I2CT, Microscopy, Nanotubes, Oxidation-Reduction, Spectrum Analysis, Team-Bianco
@article{singh_organic_2009,
title = {Organic functionalisation and characterisation of single-walled carbon nanotubes},
author = {Prabhpreet Singh and Stéphane Campidelli and Silvia Giordani and Davide Bonifazi and Alberto Bianco and Maurizio Prato},
doi = {10.1039/b518111a},
issn = {1460-4744},
year = {2009},
date = {2009-08-01},
journal = {Chemical Society Reviews},
volume = {38},
number = {8},
pages = {2214--2230},
abstract = {Since carbon nanotubes (CNTs) display unique structures and remarkable physical properties, a variety of applications have emerged in both materials and life sciences. In terms of applications, the functionalisation of nanotubes is extremely important, as it increases their solubility and processability, and combines the unique properties of single-walled carbon nanotubes (SWCNTs) with those of other classes of materials. A number of methods have been developed, which can be divided into two major approaches: (1) non-covalent supramolecular modifications, and (2) covalent functionalisation. In this tutorial review, we survey the covalent modification of SWCNTs with organic moieties, and illustrate the major analytical techniques routinely used to characterise the functionalised materials.},
keywords = {Alkylation, carbon, Esterification, Free Radicals, Halogenation, I2CT, Microscopy, Nanotubes, Oxidation-Reduction, Spectrum Analysis, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Since carbon nanotubes (CNTs) display unique structures and remarkable physical properties, a variety of applications have emerged in both materials and life sciences. In terms of applications, the functionalisation of nanotubes is extremely important, as it increases their solubility and processability, and combines the unique properties of single-walled carbon nanotubes (SWCNTs) with those of other classes of materials. A number of methods have been developed, which can be divided into two major approaches: (1) non-covalent supramolecular modifications, and (2) covalent functionalisation. In this tutorial review, we survey the covalent modification of SWCNTs with organic moieties, and illustrate the major analytical techniques routinely used to characterise the functionalised materials.
Herrero Antonia M, Toma Francesca M, Al-Jamal Khuloud T, Kostarelos Kostas, Bianco Alberto, Ros Tatiana Da, Bano Fouzia, Casalis Loredana, Scoles Giacinto, Prato Maurizio
Synthesis and characterization of a carbon nanotube-dendron series for efficient siRNA delivery Article de journal
Dans: Journal of the American Chemical Society, vol. 131, no. 28, p. 9843–9848, 2009, ISSN: 1520-5126.
Résumé | Liens | BibTeX | Étiquettes: Acrylates, Animals, Azo Compounds, Biological Transport, carbon, Cytoplasm, Dendrimers, Drug Carriers, Ethylenediamines, Gene Silencing, HeLa Cells, Humans, I2CT, Nanotubes, Polyamines, RNA, Small Interfering, Solubility, Team-Bianco, Thiosemicarbazones, Transfection, water
@article{herrero_synthesis_2009,
title = {Synthesis and characterization of a carbon nanotube-dendron series for efficient siRNA delivery},
author = {Antonia M Herrero and Francesca M Toma and Khuloud T Al-Jamal and Kostas Kostarelos and Alberto Bianco and Tatiana Da Ros and Fouzia Bano and Loredana Casalis and Giacinto Scoles and Maurizio Prato},
doi = {10.1021/ja903316z},
issn = {1520-5126},
year = {2009},
date = {2009-07-01},
journal = {Journal of the American Chemical Society},
volume = {131},
number = {28},
pages = {9843--9848},
abstract = {A new series of dendron-functionalized multiwalled carbon nanotube (MWNT) derivatives, characterized by the presence of numerous positively charged tetraalkyl ammonium salts at the periphery of the dendron, has been synthesized. The positive charges on the MWNT surface, coupled with the unique ability of carbon nanotubes (CNTs) to penetrate cell membranes, make the new derivatives potentially ideal vectors for siRNA delivery. Using a fluorescently labeled, noncoding siRNA sequence, we demonstrate that cytoplasmic delivery of the nucleic acid is remarkably increased throughout the different dendron generations. The work reported here highlights the fact that dendron-functionalized CNTs can be rationally designed as efficient carriers of siRNA that can eventually lead to gene silencing.},
keywords = {Acrylates, Animals, Azo Compounds, Biological Transport, carbon, Cytoplasm, Dendrimers, Drug Carriers, Ethylenediamines, Gene Silencing, HeLa Cells, Humans, I2CT, Nanotubes, Polyamines, RNA, Small Interfering, Solubility, Team-Bianco, Thiosemicarbazones, Transfection, water},
pubstate = {published},
tppubtype = {article}
}
A new series of dendron-functionalized multiwalled carbon nanotube (MWNT) derivatives, characterized by the presence of numerous positively charged tetraalkyl ammonium salts at the periphery of the dendron, has been synthesized. The positive charges on the MWNT surface, coupled with the unique ability of carbon nanotubes (CNTs) to penetrate cell membranes, make the new derivatives potentially ideal vectors for siRNA delivery. Using a fluorescently labeled, noncoding siRNA sequence, we demonstrate that cytoplasmic delivery of the nucleic acid is remarkably increased throughout the different dendron generations. The work reported here highlights the fact that dendron-functionalized CNTs can be rationally designed as efficient carriers of siRNA that can eventually lead to gene silencing.
Marega Riccardo, Aroulmoji Vincent, Dinon Francesca, Vaccari Lisa, Giordani Silvia, Bianco Alberto, Murano Erminio, Prato Maurizio
Diffusion-ordered NMR spectroscopy in the structural characterization of functionalized carbon nanotubes Article de journal
Dans: Journal of the American Chemical Society, vol. 131, no. 25, p. 9086–9093, 2009, ISSN: 1520-5126.
Résumé | Liens | BibTeX | Étiquettes: carbon, Diffusion, I2CT, Magnetic Resonance Spectroscopy, Nanotubes, Oxidation-Reduction, Surface Properties, Team-Bianco
@article{marega_diffusion-ordered_2009,
title = {Diffusion-ordered NMR spectroscopy in the structural characterization of functionalized carbon nanotubes},
author = {Riccardo Marega and Vincent Aroulmoji and Francesca Dinon and Lisa Vaccari and Silvia Giordani and Alberto Bianco and Erminio Murano and Maurizio Prato},
doi = {10.1021/ja902728w},
issn = {1520-5126},
year = {2009},
date = {2009-07-01},
journal = {Journal of the American Chemical Society},
volume = {131},
number = {25},
pages = {9086--9093},
abstract = {The emerging applications of functionalized carbon nanotubes (CNTs) in various research domains necessitate the use of many different analytical techniques to confirm their structural modifications in a fast and reliable manner. Thus far, NMR spectroscopy has not been among the main tools for characterization of organically modified carbon nanostructures. (1)H analysis is limited because the signals in these derivatives are typically weak and broad, resulting in uncertainties of a few parts per million, and because of the strong interference of residual solvent signals. To overcome these limitations, we investigated the applicability of proton NMR spectroscopy based on gradient-edited diffusion pulse sequences (1D diffusion-ordered spectroscopy, DOSY) in the characterization of CNT derivatives. In general, diffusion NMR experiments allow the separation of NMR signals of different species present in a mixture, according to their own diffusion coefficients, merging spectroscopy information with size analysis. In the present study, a selected set of CNT derivatives was synthesized and analyzed using 1D DOSY experiments by applying strong magnetic field gradients (up to 42.6 G cm(-1)). Colorimetric tests (i.e., Kaiser test) and TGA analysis support the NMR findings, which are related to isolated and/or bundled short SWNTs, on the basis of TEM and AFM characterization. The overall results show that the diffusion-based NMR spectroscopy is a fast and promising approach for the characterization of covalently modified CNT derivatives.},
keywords = {carbon, Diffusion, I2CT, Magnetic Resonance Spectroscopy, Nanotubes, Oxidation-Reduction, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
The emerging applications of functionalized carbon nanotubes (CNTs) in various research domains necessitate the use of many different analytical techniques to confirm their structural modifications in a fast and reliable manner. Thus far, NMR spectroscopy has not been among the main tools for characterization of organically modified carbon nanostructures. (1)H analysis is limited because the signals in these derivatives are typically weak and broad, resulting in uncertainties of a few parts per million, and because of the strong interference of residual solvent signals. To overcome these limitations, we investigated the applicability of proton NMR spectroscopy based on gradient-edited diffusion pulse sequences (1D diffusion-ordered spectroscopy, DOSY) in the characterization of CNT derivatives. In general, diffusion NMR experiments allow the separation of NMR signals of different species present in a mixture, according to their own diffusion coefficients, merging spectroscopy information with size analysis. In the present study, a selected set of CNT derivatives was synthesized and analyzed using 1D DOSY experiments by applying strong magnetic field gradients (up to 42.6 G cm(-1)). Colorimetric tests (i.e., Kaiser test) and TGA analysis support the NMR findings, which are related to isolated and/or bundled short SWNTs, on the basis of TEM and AFM characterization. The overall results show that the diffusion-based NMR spectroscopy is a fast and promising approach for the characterization of covalently modified CNT derivatives.
Podesta Jennifer E, Al-Jamal Khuloud T, Herrero Antonia M, Tian Bowen, Ali-Boucetta Hanene, Hegde Vikas, Bianco Alberto, Prato Maurizio, Kostarelos Kostas
Antitumor activity and prolonged survival by carbon-nanotube-mediated therapeutic siRNA silencing in a human lung xenograft model Article de journal
Dans: Small (Weinheim an Der Bergstrasse, Germany), vol. 5, no. 10, p. 1176–1185, 2009, ISSN: 1613-6829.
Résumé | Liens | BibTeX | Étiquettes: Animals, Antineoplastic Agents, Apoptosis, carbon, Cell Line, Cell Proliferation, Electrophoresis, Gene Silencing, Humans, I2CT, Liposomes, Lung Neoplasms, Mice, Nanomedicine, Nanotubes, RNA, Small Interfering, Survival Analysis, Team-Bianco, tumor, Xenograft Model Antitumor Assays
@article{podesta_antitumor_2009,
title = {Antitumor activity and prolonged survival by carbon-nanotube-mediated therapeutic siRNA silencing in a human lung xenograft model},
author = {Jennifer E Podesta and Khuloud T Al-Jamal and Antonia M Herrero and Bowen Tian and Hanene Ali-Boucetta and Vikas Hegde and Alberto Bianco and Maurizio Prato and Kostas Kostarelos},
doi = {10.1002/smll.200801572},
issn = {1613-6829},
year = {2009},
date = {2009-05-01},
journal = {Small (Weinheim an Der Bergstrasse, Germany)},
volume = {5},
number = {10},
pages = {1176--1185},
abstract = {Carbon nanotubes are novel nanomaterials that are thought to offer potential benefits to a variety of biomedical and clinical applications. In this study, the treatment of a human lung carcinoma model in vivo using siRNA sequences leading to cytotoxicity and cell death is carried out using either cationic liposomes (DOTAP:cholesterol) or amino-functionalized multi-walled carbon nanotubes (MWNT - NH(+)(3)). Validation for the most cytotoxic siRNA sequence using a panel of human carcinoma and murine cells reveals that the proprietary siTOX sequence is human specific and can lead to significant cytotoxic activities delivered both by liposome or MWNT - NH(+)(3) in vitro. A comparative study using both types of vector indicates that only MWNT - NH(+)(3):siRNA complexes administered intratumorally can elicit delayed tumor growth and increased survival of xenograft-bearing animals. siTOX delivery via the cationic MWNT - NH(+)(3) is biologically active in vivo by triggering an apoptotic cascade, leading to extensive necrosis of the human tumor mass. This suggests that carbon-nanotube-mediated delivery of siRNA by intratumoral administration leads to successful and statistically significant suppression of tumor volume, followed by a concomitant prolongation of survival of human lung tumor-bearing animals. The direct comparison between carbon nanotubes and liposomes demonstrates the potential advantages offered by carbon nanotubes for the intracellular delivery of therapeutic agents in vivo. The present work may act as the impetus for further studies to explore the therapeutic capacity of chemically functionalized carbon nanotubes to deliver siRNA directly into the cytoplasm of target cells and achieve effective therapeutic silencing in various disease indications where local delivery is feasible or desirable.},
keywords = {Animals, Antineoplastic Agents, Apoptosis, carbon, Cell Line, Cell Proliferation, Electrophoresis, Gene Silencing, Humans, I2CT, Liposomes, Lung Neoplasms, Mice, Nanomedicine, Nanotubes, RNA, Small Interfering, Survival Analysis, Team-Bianco, tumor, Xenograft Model Antitumor Assays},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes are novel nanomaterials that are thought to offer potential benefits to a variety of biomedical and clinical applications. In this study, the treatment of a human lung carcinoma model in vivo using siRNA sequences leading to cytotoxicity and cell death is carried out using either cationic liposomes (DOTAP:cholesterol) or amino-functionalized multi-walled carbon nanotubes (MWNT - NH(+)(3)). Validation for the most cytotoxic siRNA sequence using a panel of human carcinoma and murine cells reveals that the proprietary siTOX sequence is human specific and can lead to significant cytotoxic activities delivered both by liposome or MWNT - NH(+)(3) in vitro. A comparative study using both types of vector indicates that only MWNT - NH(+)(3):siRNA complexes administered intratumorally can elicit delayed tumor growth and increased survival of xenograft-bearing animals. siTOX delivery via the cationic MWNT - NH(+)(3) is biologically active in vivo by triggering an apoptotic cascade, leading to extensive necrosis of the human tumor mass. This suggests that carbon-nanotube-mediated delivery of siRNA by intratumoral administration leads to successful and statistically significant suppression of tumor volume, followed by a concomitant prolongation of survival of human lung tumor-bearing animals. The direct comparison between carbon nanotubes and liposomes demonstrates the potential advantages offered by carbon nanotubes for the intracellular delivery of therapeutic agents in vivo. The present work may act as the impetus for further studies to explore the therapeutic capacity of chemically functionalized carbon nanotubes to deliver siRNA directly into the cytoplasm of target cells and achieve effective therapeutic silencing in various disease indications where local delivery is feasible or desirable.
Bianco Alberto
Potential usefulness of carbon nanotubes for cancer therapy Article de journal
Dans: Medecine Sciences: M/S, vol. 25, no. 2, p. 125–127, 2009, ISSN: 0767-0974.
Liens | BibTeX | Étiquettes: carbon, Graphite, Humans, I2CT, Nanotubes, Neoplasms, Team-Bianco
@article{bianco_potential_2009,
title = {Potential usefulness of carbon nanotubes for cancer therapy},
author = {Alberto Bianco},
doi = {10.1051/medsci/2009252125},
issn = {0767-0974},
year = {2009},
date = {2009-02-01},
journal = {Medecine Sciences: M/S},
volume = {25},
number = {2},
pages = {125--127},
keywords = {carbon, Graphite, Humans, I2CT, Nanotubes, Neoplasms, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
2008
Lacerda Lara, Herrero Maria A, Venner Kerrie, Bianco Alberto, Prato Maurizio, Kostarelos Kostas
Carbon-nanotube shape and individualization critical for renal excretion Article de journal
Dans: Small (Weinheim an Der Bergstrasse, Germany), vol. 4, no. 8, p. 1130–1132, 2008, ISSN: 1613-6829.
Liens | BibTeX | Étiquettes: Animals, Biological Transport, carbon, Electron, Female, Glomerular Filtration Rate, I2CT, Inbred BALB C, Kidney Glomerulus, Mice, Microscopy, Nanoparticles, nanotechnology, Nanotubes, Team-Bianco, Transmission
@article{lacerda_carbon-nanotube_2008,
title = {Carbon-nanotube shape and individualization critical for renal excretion},
author = {Lara Lacerda and Maria A Herrero and Kerrie Venner and Alberto Bianco and Maurizio Prato and Kostas Kostarelos},
doi = {10.1002/smll.200800323},
issn = {1613-6829},
year = {2008},
date = {2008-08-01},
journal = {Small (Weinheim an Der Bergstrasse, Germany)},
volume = {4},
number = {8},
pages = {1130--1132},
keywords = {Animals, Biological Transport, carbon, Electron, Female, Glomerular Filtration Rate, I2CT, Inbred BALB C, Kidney Glomerulus, Mice, Microscopy, Nanoparticles, nanotechnology, Nanotubes, Team-Bianco, Transmission},
pubstate = {published},
tppubtype = {article}
}
Fabre Bruno, Hauquier Fanny, Herrier Cyril, Pastorin Giorgia, Wu Wei, Bianco Alberto, Prato Maurizio, Hapiot Philippe, Zigah Dodzi, Prasciolu Mauro, Vaccari Lisa
Covalent assembly and micropatterning of functionalized multiwalled carbon nanotubes to monolayer-modified Si(111) surfaces Article de journal
Dans: Langmuir: the ACS journal of surfaces and colloids, vol. 24, no. 13, p. 6595–6602, 2008, ISSN: 0743-7463.
Résumé | Liens | BibTeX | Étiquettes: Atomic Force, carbon, Electrochemistry, Electron, I2CT, Microscopy, Nanotubes, scanning, Silicon, Surface Properties, Team-Bianco
@article{fabre_covalent_2008,
title = {Covalent assembly and micropatterning of functionalized multiwalled carbon nanotubes to monolayer-modified Si(111) surfaces},
author = {Bruno Fabre and Fanny Hauquier and Cyril Herrier and Giorgia Pastorin and Wei Wu and Alberto Bianco and Maurizio Prato and Philippe Hapiot and Dodzi Zigah and Mauro Prasciolu and Lisa Vaccari},
doi = {10.1021/la800358w},
issn = {0743-7463},
year = {2008},
date = {2008-06-01},
journal = {Langmuir: the ACS journal of surfaces and colloids},
volume = {24},
number = {13},
pages = {6595--6602},
abstract = {Multiwalled carbon nanotubes (MWNTs) covalently bound to monocrystalline p-type Si(111) surfaces have been prepared by attaching soluble amine-functionalized MWNTs onto a preassembled undecanoic acid monolayer using carbodiimide coupling. SEM analysis of these functionalized surfaces shows that the bound MWNTs are parallel to the surface rather than perpendicular. The voltammetric and electrochemical impedance spectroscopy measurements reveal that the electron transfer at the MWNT-modified surface is faster than that observed at a MWNT-free alkyl monolayer. We have also demonstrated that it is possible to prepare MWNT micropatterns using this surface amidation reaction and a "reagentless" UV photolithography technique. Following this approach, MWNT patterns surrounded by n-dodecyl areas have been produced and the local electrochemical properties of these micropatterned surfaces have been examined by scanning electrochemical microscopy. In particular, it is demonstrated that the MWNT patterns allow a faster charge transfer which is consistent with the results obtained for the uniformly modified surfaces.},
keywords = {Atomic Force, carbon, Electrochemistry, Electron, I2CT, Microscopy, Nanotubes, scanning, Silicon, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Multiwalled carbon nanotubes (MWNTs) covalently bound to monocrystalline p-type Si(111) surfaces have been prepared by attaching soluble amine-functionalized MWNTs onto a preassembled undecanoic acid monolayer using carbodiimide coupling. SEM analysis of these functionalized surfaces shows that the bound MWNTs are parallel to the surface rather than perpendicular. The voltammetric and electrochemical impedance spectroscopy measurements reveal that the electron transfer at the MWNT-modified surface is faster than that observed at a MWNT-free alkyl monolayer. We have also demonstrated that it is possible to prepare MWNT micropatterns u