Publications
2009
Dhouib K, Malek C Khan, Pfleging W, Gauthier-Manuel B, Duffait R, Thuillier G, Ferrigno R, Jacquamet L, Ohana J, Ferrer J L, Théobald-Dietrich A, Giege R, Lorber B, Sauter C
Microfluidic chips for the crystallization of biomacromolecules by counter-diffusion and on-chip crystal X-ray analysis Article de journal
Dans: Lab Chip, vol. 9, no. 10, p. 1412-1421, 2009, ISBN: 19417908, (1473-0197 (Print) 1473-0189 (Linking) Journal Article Research Support, Non-U.S. Gov't).
Résumé | Liens | BibTeX | Étiquettes: FRUGIER, SAUTER, Unité ARN, X-Ray/*instrumentation Dimethylpolysiloxanes/chemistry Macromolecular Substances/*chemistry Microfluidic Analytical Techniques/*instrumentation Polymethyl Methacrylate/chemistry
@article{,
title = {Microfluidic chips for the crystallization of biomacromolecules by counter-diffusion and on-chip crystal X-ray analysis},
author = {K Dhouib and C Khan Malek and W Pfleging and B Gauthier-Manuel and R Duffait and G Thuillier and R Ferrigno and L Jacquamet and J Ohana and J L Ferrer and A Théobald-Dietrich and R Giege and B Lorber and C Sauter},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19417908},
isbn = {19417908},
year = {2009},
date = {2009-01-01},
journal = {Lab Chip},
volume = {9},
number = {10},
pages = {1412-1421},
abstract = {Microfluidic devices were designed to perform on micromoles of biological macromolecules and viruses the search and the optimization of crystallization conditions by counter-diffusion, as well as the on-chip analysis of crystals by X-ray diffraction. Chips composed of microchannels were fabricated in poly-dimethylsiloxane (PDMS), poly-methyl-methacrylate (PMMA) and cyclo-olefin-copolymer (COC) by three distinct methods, namely replica casting, laser ablation and hot embossing. The geometry of the channels was chosen to ensure that crystallization occurs in a convection-free environment. The transparency of the materials is compatible with crystal growth monitoring by optical microscopy. The quality of the protein 3D structures derived from on-chip crystal analysis by X-ray diffraction using a synchrotron radiation was used to identify the most appropriate polymers. Altogether the results demonstrate that for a novel biomolecule, all steps from the initial search of crystallization conditions to X-ray diffraction data collection for 3D structure determination can be performed in a single chip.},
note = {1473-0197 (Print)
1473-0189 (Linking)
Journal Article
Research Support, Non-U.S. Gov't},
keywords = {FRUGIER, SAUTER, Unité ARN, X-Ray/*instrumentation Dimethylpolysiloxanes/chemistry Macromolecular Substances/*chemistry Microfluidic Analytical Techniques/*instrumentation Polymethyl Methacrylate/chemistry},
pubstate = {published},
tppubtype = {article}
}
Microfluidic devices were designed to perform on micromoles of biological macromolecules and viruses the search and the optimization of crystallization conditions by counter-diffusion, as well as the on-chip analysis of crystals by X-ray diffraction. Chips composed of microchannels were fabricated in poly-dimethylsiloxane (PDMS), poly-methyl-methacrylate (PMMA) and cyclo-olefin-copolymer (COC) by three distinct methods, namely replica casting, laser ablation and hot embossing. The geometry of the channels was chosen to ensure that crystallization occurs in a convection-free environment. The transparency of the materials is compatible with crystal growth monitoring by optical microscopy. The quality of the protein 3D structures derived from on-chip crystal analysis by X-ray diffraction using a synchrotron radiation was used to identify the most appropriate polymers. Altogether the results demonstrate that for a novel biomolecule, all steps from the initial search of crystallization conditions to X-ray diffraction data collection for 3D structure determination can be performed in a single chip.