Publications
2009
Chevalier C, Geissmann T, Helfer A C, Romby P
Probing mRNA structure and sRNA-mRNA interactions in bacteria using enzymes and lead(II) Chapitre d'ouvrage
Dans: Serganov, A (Ed.): Riboswitches: Methods and Protocols, vol. 540, p. 215-232, Springer Protocols, Humana Press, 2009, ISBN: 19381563.
Résumé | Liens | BibTeX | Étiquettes: Bacterial/chemical synthesis RNA, Base Sequence Chemical Fractionation Enzymes/*metabolism Hydrolysis/drug effects Lead/*pharmacology Molecular Biology/*methods Molecular Sequence Data Nucleic Acid Conformation/drug effects RNA/metabolism RNA, Messenger/*chemistry/genetics/*metabolism RNA, ROMBY, Unité ARN, Untranslated/chemistry/genetics/*metabolism Staphylococcus aureus/drug effects/*metabolism
@inbook{,
title = {Probing mRNA structure and sRNA-mRNA interactions in bacteria using enzymes and lead(II)},
author = {C Chevalier and T Geissmann and A C Helfer and P Romby},
editor = {A Serganov},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19381563},
doi = {978-1-59745-558-9_16},
isbn = {19381563},
year = {2009},
date = {2009-01-01},
booktitle = {Riboswitches: Methods and Protocols},
volume = {540},
pages = {215-232},
publisher = {Springer Protocols, Humana Press},
series = {Methods in Molecular Biology},
abstract = {Enzymatic probing and lead(II)-induced cleavages have been developed to study the secondary structure of RNA molecules either free or engaged in complex with different ligands. Using a combination of probes with different specificities (unpaired vs. paired regions), it is possible to get information on the accessibility of each nucleotide, on the binding site of a ligand (noncoding RNAs, protein, metabolites), and on RNA conformational changes that accompanied ligand binding or environmental conditions (temperature, pH, ions, etc.). The detection of the cleavages can be conducted by two different ways, which are chosen according to the length of the studied RNA. The first method uses end-labeled RNA molecules and the second one involves primer extension by reverse transcriptase. We provide here an experimental procedure that was designed to map the structure of mRNA and mRNA-sRNA interaction in vitro.},
keywords = {Bacterial/chemical synthesis RNA, Base Sequence Chemical Fractionation Enzymes/*metabolism Hydrolysis/drug effects Lead/*pharmacology Molecular Biology/*methods Molecular Sequence Data Nucleic Acid Conformation/drug effects RNA/metabolism RNA, Messenger/*chemistry/genetics/*metabolism RNA, ROMBY, Unité ARN, Untranslated/chemistry/genetics/*metabolism Staphylococcus aureus/drug effects/*metabolism},
pubstate = {published},
tppubtype = {inbook}
}
Enzymatic probing and lead(II)-induced cleavages have been developed to study the secondary structure of RNA molecules either free or engaged in complex with different ligands. Using a combination of probes with different specificities (unpaired vs. paired regions), it is possible to get information on the accessibility of each nucleotide, on the binding site of a ligand (noncoding RNAs, protein, metabolites), and on RNA conformational changes that accompanied ligand binding or environmental conditions (temperature, pH, ions, etc.). The detection of the cleavages can be conducted by two different ways, which are chosen according to the length of the studied RNA. The first method uses end-labeled RNA molecules and the second one involves primer extension by reverse transcriptase. We provide here an experimental procedure that was designed to map the structure of mRNA and mRNA-sRNA interaction in vitro.