Réponses antivirales chez le moustique Aedes

@article{,

title = {Structural specificity of Rn nuclease I as probed on yeast tRNA(Phe) and tRNA(Asp)},

author = { A. Przykorska and C. el Adlouni and G. Keith and J. W. Szarkowski and G. Dirheimer},

year  = {1992},

date = {1992-01-01},

journal = {Nucleic Acids Res},

volume = {20},

number = {4},

pages = {659-63},

abstract = {A single-strand-specific nuclease from rye germ (Rn nuclease I) was characterized as a tool for secondary and tertiary structure investigation of RNAs. To test the procedure, yeast tRNA(Phe) and tRNA(Asp) for which the tertiary structures are known, as well as the 3'-half of tRNA(Asp) were used as substrates. In tRNA(Phe) the nuclease introduced main primary cuts at positions U33 and A35 of the anticodon loop and G18 and G19 of the D loop. No primary cuts were observed within the double stranded stems. In tRNA(Asp) the main cuts occurred at positions U33, G34, U35, C36 of the anticodon loop and G18 and C20:1 positions in the D loop. No cuts were observed in the T loop in intact tRNA(Asp) but strong primary cleavages occurred at positions psi 55, C56, A57 within that loop in the absence of the tertiary interactions between T and D loops (use of 3'-half tRNA(Asp)). These results show that Rn nuclease I is specific for exposed single-stranded regions.},

note = {0305-1048 

Journal Article},

keywords = {Acid, Asp/chemistry/genetics/*metabolism, Base, cereale, Composition, Conformation, Data, Gov't, Molecular, Non-U.S., Nucleic, Pancreatic/*metabolism, Phe/chemistry/genetics/*metabolism, Ribonuclease, RNA, Secale, Sequence, Specificity, Substrate, Support, Transfer, Yeasts/genetics},

pubstate = {published},

tppubtype = {article}

}

@article{,

title = {Rapid transfer of small RNAs from a polyacrylamide gel onto a nylon membrane using a gel dryer},

author = { M. L. Wilhelm and W. Baranowski and G. Keith and F. X. Wilhelm},

year  = {1992},

date = {1992-01-01},

journal = {Nucleic Acids Res},

volume = {20},

number = {15},

pages = {4106},

note = {0305-1048 

Journal Article},

keywords = {&, 5S/*isolation, Acrylic, Human, Nuclear/*isolation, Nylons, purification, Resins, Ribosomal, RNA, Small, Transfer/*isolation, Yeasts/genetics},

pubstate = {published},

tppubtype = {article}

}