Publications
2012
Dehuyser L, Schaeffer E, Chaloin O, Mueller C G, Baati R, Wagner A
Synthesis of Novel Mannoside Glycolipid Conjugates for Inhibition of HIV-1 Trans-Infection Article de journal
Dans: Bioconjug.Chem., no. 1520-4812 (Electronic), 2012.
Résumé | BibTeX | Étiquettes: Dendritic Cells, HIV-1, Human, immunodeficiency, infection, inhibition, LECTIN, Lectins, lipid, Mannose-Binding Lectins, prevention, Solubility, Surface Plasmon Resonance, synthesis, Team-Mueller, virus
@article{dehuyser_synthesis_2012,
title = {Synthesis of Novel Mannoside Glycolipid Conjugates for Inhibition of HIV-1 Trans-Infection},
author = {L Dehuyser and E Schaeffer and O Chaloin and C G Mueller and R Baati and A Wagner},
year = {2012},
date = {2012-01-01},
journal = {Bioconjug.Chem.},
number = {1520-4812 (Electronic)},
abstract = {Mannose-binding lectins, such as dendritic cell-specific ICAM-3-grabbing non-integrin (DC-SIGN), are expressed at the surface of human dendritic cells (DCs) that capture and transmit human immunodeficiency virus type-1 (HIV-1) to CD4(+) cells. With the goal of reducing viral trans-infection by targeting DC-SIGN, we have designed a new class of mannoside glycolipid conjugates. We report the synthesis of amphiphiles composed of a mannose head, a hydrophilic linker essential for solubility in aqueous media, and a lipid chain of variable length. These conjugates presented unusual properties based on a cooperation between the mannoside head and the lipid chain, which enhanced the affinity and decreased the need for multivalency. With an optimal lipid length, they exhibited strong binding affinity for DC-SIGN (K(d) in the micromolar range) as assessed by surface plasmon resonance. The most active molecules were branched trimannoside conjugates, able to inhibit the interaction of the HIV-1 envelope with DCs, and to drastically reduce trans-infection of HIV-1 mediated by DCs (IC(50s) in the low micromolar range). This new class of compounds may be of potential use for prevention of HIV-1 dissemination, and also of infection by other DC-SIGN-binding human pathogens},
keywords = {Dendritic Cells, HIV-1, Human, immunodeficiency, infection, inhibition, LECTIN, Lectins, lipid, Mannose-Binding Lectins, prevention, Solubility, Surface Plasmon Resonance, synthesis, Team-Mueller, virus},
pubstate = {published},
tppubtype = {article}
}
2011
Nehme Nadine T, Quintin Jessica, Cho Ju Hyun, Lee Janice, Lafarge Marie-Céline, Kocks Christine, Ferrandon Dominique
Relative roles of the cellular and humoral responses in the Drosophila host defense against three gram-positive bacterial infections Article de journal
Dans: PLoS ONE, vol. 6, no. 3, p. e14743, 2011, ISSN: 1932-6203.
Résumé | Liens | BibTeX | Étiquettes: Animals, Antimicrobial Cationic Peptides, Carrier Proteins, Cell Surface, Cellular, Enterococcus faecalis, ferrandon, Gram-Positive Bacteria, Gram-Positive Bacterial Infections, Host-Pathogen Interactions, Humoral, Immunity, Innate, M3i, Micrococcus luteus, Opsonin Proteins, Phagocytosis, Receptors, Signal Transduction, Solubility, Staphylococcus aureus
@article{nehme_relative_2011b,
title = {Relative roles of the cellular and humoral responses in the Drosophila host defense against three gram-positive bacterial infections},
author = {Nadine T Nehme and Jessica Quintin and Ju Hyun Cho and Janice Lee and Marie-Céline Lafarge and Christine Kocks and Dominique Ferrandon},
doi = {10.1371/journal.pone.0014743},
issn = {1932-6203},
year = {2011},
date = {2011-01-01},
journal = {PLoS ONE},
volume = {6},
number = {3},
pages = {e14743},
abstract = {BACKGROUND: Two NF-kappaB signaling pathways, Toll and immune deficiency (imd), are required for survival to bacterial infections in Drosophila. In response to septic injury, these pathways mediate rapid transcriptional activation of distinct sets of effector molecules, including antimicrobial peptides, which are important components of a humoral defense response. However, it is less clear to what extent macrophage-like hemocytes contribute to host defense. METHODOLOGY/PRINCIPAL FINDINGS: In order to dissect the relative importance of humoral and cellular defenses after septic injury with three different gram-positive bacteria (Micrococcus luteus, Enterococcus faecalis, Staphylococcus aureus), we used latex bead pre-injection to ablate macrophage function in flies wildtype or mutant for various Toll and imd pathway components. We found that in all three infection models a compromised phagocytic system impaired fly survival--independently of concomitant Toll or imd pathway activation. Our data failed to confirm a role of the PGRP-SA and GNBP1 Pattern Recognition Receptors for phagocytosis of S. aureus. The Drosophila scavenger receptor Eater mediates the phagocytosis by hemocytes or S2 cells of E. faecalis and S. aureus, but not of M. luteus. In the case of M. luteus and E. faecalis, but not S. aureus, decreased survival due to defective phagocytosis could be compensated for by genetically enhancing the humoral immune response. CONCLUSIONS/SIGNIFICANCE: Our results underscore the fundamental importance of both cellular and humoral mechanisms in Drosophila immunity and shed light on the balance between these two arms of host defense depending on the invading pathogen.},
keywords = {Animals, Antimicrobial Cationic Peptides, Carrier Proteins, Cell Surface, Cellular, Enterococcus faecalis, ferrandon, Gram-Positive Bacteria, Gram-Positive Bacterial Infections, Host-Pathogen Interactions, Humoral, Immunity, Innate, M3i, Micrococcus luteus, Opsonin Proteins, Phagocytosis, Receptors, Signal Transduction, Solubility, Staphylococcus aureus},
pubstate = {published},
tppubtype = {article}
}
2010
Marega Riccardo, Aroulmoji Vincent, Bergamin Massimo, Feruglio Luigi, Dinon Francesca, Bianco Alberto, Murano Erminio, Prato Maurizio
Two-dimensional diffusion-ordered NMR spectroscopy as a tool for monitoring functionalized carbon nanotube purification and composition Article de journal
Dans: ACS nano, vol. 4, no. 4, p. 2051–2058, 2010, ISSN: 1936-086X.
Résumé | Liens | BibTeX | Étiquettes: carbon, Diffusion, I2CT, Magnetic Resonance Spectroscopy, Nanotubes, Polyethylene Glycols, Solubility, Team-Bianco, Temperature, water
@article{marega_two-dimensional_2010,
title = {Two-dimensional diffusion-ordered NMR spectroscopy as a tool for monitoring functionalized carbon nanotube purification and composition},
author = {Riccardo Marega and Vincent Aroulmoji and Massimo Bergamin and Luigi Feruglio and Francesca Dinon and Alberto Bianco and Erminio Murano and Maurizio Prato},
doi = {10.1021/nn100257h},
issn = {1936-086X},
year = {2010},
date = {2010-04-01},
journal = {ACS nano},
volume = {4},
number = {4},
pages = {2051--2058},
abstract = {Functionalized carbon nanotube (CNT) derivatives are currently under thorough investigation in different biomedical investigations. In this field of research, the composition of sample either in terms of covalently attached or physisorbed moieties can greatly affect the observed results and hamper the comparison between different studies. Therefore, the availability of a fast and reliable analytical technique to assess both the type of interaction (covalent vs noncovalent) and the composition of CNT conjugates is of great importance. Here we describe that the two-dimensional diffusion-ordered (DOSY) NMR spectroscopy is extremely useful to discriminate between conjugated and unconjugated polyethylene glycol groups in samples obtained by condensation with oxidized single-walled carbon nanotubes (SWNTs). This fast and nondestructive technique allows us to follow the removal of unconjugated polyethylene glycol chains during the purification. In particular, DOSY analysis reveal that about 1/3 (wt %) of the polyethylene glycol used for the condensation remained physisorbed to functionalized SWNTs after dialysis. Complete elimination of physisorbed polyethylene glycol was achieved using diafiltration.},
keywords = {carbon, Diffusion, I2CT, Magnetic Resonance Spectroscopy, Nanotubes, Polyethylene Glycols, Solubility, Team-Bianco, Temperature, water},
pubstate = {published},
tppubtype = {article}
}
2009
Herrero Antonia M, Toma Francesca M, Al-Jamal Khuloud T, Kostarelos Kostas, Bianco Alberto, Ros Tatiana Da, Bano Fouzia, Casalis Loredana, Scoles Giacinto, Prato Maurizio
Synthesis and characterization of a carbon nanotube-dendron series for efficient siRNA delivery Article de journal
Dans: Journal of the American Chemical Society, vol. 131, no. 28, p. 9843–9848, 2009, ISSN: 1520-5126.
Résumé | Liens | BibTeX | Étiquettes: Acrylates, Animals, Azo Compounds, Biological Transport, carbon, Cytoplasm, Dendrimers, Drug Carriers, Ethylenediamines, Gene Silencing, HeLa Cells, Humans, I2CT, Nanotubes, Polyamines, RNA, Small Interfering, Solubility, Team-Bianco, Thiosemicarbazones, Transfection, water
@article{herrero_synthesis_2009,
title = {Synthesis and characterization of a carbon nanotube-dendron series for efficient siRNA delivery},
author = {Antonia M Herrero and Francesca M Toma and Khuloud T Al-Jamal and Kostas Kostarelos and Alberto Bianco and Tatiana Da Ros and Fouzia Bano and Loredana Casalis and Giacinto Scoles and Maurizio Prato},
doi = {10.1021/ja903316z},
issn = {1520-5126},
year = {2009},
date = {2009-07-01},
journal = {Journal of the American Chemical Society},
volume = {131},
number = {28},
pages = {9843--9848},
abstract = {A new series of dendron-functionalized multiwalled carbon nanotube (MWNT) derivatives, characterized by the presence of numerous positively charged tetraalkyl ammonium salts at the periphery of the dendron, has been synthesized. The positive charges on the MWNT surface, coupled with the unique ability of carbon nanotubes (CNTs) to penetrate cell membranes, make the new derivatives potentially ideal vectors for siRNA delivery. Using a fluorescently labeled, noncoding siRNA sequence, we demonstrate that cytoplasmic delivery of the nucleic acid is remarkably increased throughout the different dendron generations. The work reported here highlights the fact that dendron-functionalized CNTs can be rationally designed as efficient carriers of siRNA that can eventually lead to gene silencing.},
keywords = {Acrylates, Animals, Azo Compounds, Biological Transport, carbon, Cytoplasm, Dendrimers, Drug Carriers, Ethylenediamines, Gene Silencing, HeLa Cells, Humans, I2CT, Nanotubes, Polyamines, RNA, Small Interfering, Solubility, Team-Bianco, Thiosemicarbazones, Transfection, water},
pubstate = {published},
tppubtype = {article}
}
2006
Tasis Dimitrios, Tagmatarchis Nikos, Bianco Alberto, Prato Maurizio
Chemistry of carbon nanotubes Article de journal
Dans: Chemical Reviews, vol. 106, no. 3, p. 1105–1136, 2006, ISSN: 0009-2665.
Liens | BibTeX | Étiquettes: Animals, Biopolymers, carbon, Chemical Phenomena, Chemistry, I2CT, Molecular Structure, Nanotubes, Solubility, Surface Properties, Team-Bianco
@article{tasis_chemistry_2006,
title = {Chemistry of carbon nanotubes},
author = {Dimitrios Tasis and Nikos Tagmatarchis and Alberto Bianco and Maurizio Prato},
doi = {10.1021/cr050569o},
issn = {0009-2665},
year = {2006},
date = {2006-03-01},
journal = {Chemical Reviews},
volume = {106},
number = {3},
pages = {1105--1136},
keywords = {Animals, Biopolymers, carbon, Chemical Phenomena, Chemistry, I2CT, Molecular Structure, Nanotubes, Solubility, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Pastorin Giorgia, Wu Wei, Wieckowski Sébastien, Briand Jean-Paul, Kostarelos Kostas, Prato Maurizio, Bianco Alberto
Double functionalization of carbon nanotubes for multimodal drug delivery Article de journal
Dans: Chemical Communications (Cambridge, England), no. 11, p. 1182–1184, 2006, ISSN: 1359-7345.
Résumé | Liens | BibTeX | Étiquettes: Azo Compounds, carbon, Cyclization, Drug Carriers, I2CT, Microscopy, Nanotubes, Pharmaceutical Preparations, Scanning Tunneling, Solubility, Team-Bianco
@article{pastorin_double_2006,
title = {Double functionalization of carbon nanotubes for multimodal drug delivery},
author = {Giorgia Pastorin and Wei Wu and Sébastien Wieckowski and Jean-Paul Briand and Kostas Kostarelos and Maurizio Prato and Alberto Bianco},
doi = {10.1039/b516309a},
issn = {1359-7345},
year = {2006},
date = {2006-03-01},
journal = {Chemical Communications (Cambridge, England)},
number = {11},
pages = {1182--1184},
abstract = {Multi-walled carbon nanotubes have been covalently functionalized via 1,3-dipolar cycloaddition of azomethine ylides with orthogonally protected amino functions that can be selectively deprotected and subsequently modified with drugs and fluorescent probes.},
keywords = {Azo Compounds, carbon, Cyclization, Drug Carriers, I2CT, Microscopy, Nanotubes, Pharmaceutical Preparations, Scanning Tunneling, Solubility, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
2005
Wu Wei, Wieckowski Sébastien, Pastorin Giorgia, Benincasa Monica, Klumpp Cédric, Briand Jean-Paul, Gennaro Renato, Prato Maurizio, Bianco Alberto
Targeted delivery of amphotericin B to cells by using functionalized carbon nanotubes Article de journal
Dans: Angewandte Chemie (International Ed. in English), vol. 44, no. 39, p. 6358–6362, 2005, ISSN: 1433-7851.
Liens | BibTeX | Étiquettes: Amphotericin B, Antifungal Agents, carbon, Drug Carriers, Drug Delivery Systems, Fungi, Humans, I2CT, Jurkat Cells, Molecular Structure, Nanotubes, Particle Size, Solubility, Surface Properties, Team-Bianco
@article{wu_targeted_2005,
title = {Targeted delivery of amphotericin B to cells by using functionalized carbon nanotubes},
author = {Wei Wu and Sébastien Wieckowski and Giorgia Pastorin and Monica Benincasa and Cédric Klumpp and Jean-Paul Briand and Renato Gennaro and Maurizio Prato and Alberto Bianco},
doi = {10.1002/anie.200501613},
issn = {1433-7851},
year = {2005},
date = {2005-10-01},
journal = {Angewandte Chemie (International Ed. in English)},
volume = {44},
number = {39},
pages = {6358--6362},
keywords = {Amphotericin B, Antifungal Agents, carbon, Drug Carriers, Drug Delivery Systems, Fungi, Humans, I2CT, Jurkat Cells, Molecular Structure, Nanotubes, Particle Size, Solubility, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
2004
Pantarotto Davide, Tagmatarchis Nikos, Bianco Alberto, Prato Maurizio
Synthesis and biological properties of fullerene-containing amino acids and peptides Article de journal
Dans: Mini Reviews in Medicinal Chemistry, vol. 4, no. 7, p. 805–814, 2004, ISSN: 1389-5575.
Résumé | BibTeX | Étiquettes: Amino Acids, Animals, Fullerenes, Humans, I2CT, Molecular Structure, Peptides, Solubility, Team-Bianco
@article{pantarotto_synthesis_2004,
title = {Synthesis and biological properties of fullerene-containing amino acids and peptides},
author = {Davide Pantarotto and Nikos Tagmatarchis and Alberto Bianco and Maurizio Prato},
issn = {1389-5575},
year = {2004},
date = {2004-09-01},
journal = {Mini Reviews in Medicinal Chemistry},
volume = {4},
number = {7},
pages = {805--814},
abstract = {Organofullerene derivatives have shown a great potential in a wide variety of biological activities such as DNA photocleavage, HIV-protease inhibition, neuroprotection and apoptosis. Among the plethora of functionalized organofullerenes that have been synthesized, fullerene-based amino acids are particularly appealing for structural studies and biological applications. When the fullerene-framework is incorporated into peptides, its original properties can be substantially modified. In addition, the water-solubility of the fullerene derivatives is enhanced, which makes such molecules amenable to biological studies. In this review, recent advances in the growing field of medicinal chemistry of fullerene derivatives will be discussed. Emphasis will be given to the synthesis of the biggest unnatural amino acid 3,4-fulleroproline (Fpr) and its derivatives. For example, Fpr derivatives have been found to interact with different hydrolytic enzymes and selectively discriminate between rationally designed peptides. Fullerene-based peptides have been found to substantially activate enzymes involved in the oxidative deamination of biogenic amines. In addition, their membranotropic properties and effects on the structure and permeability of the lipid bilayer of phosphatidylcholine liposomes as well as the transmembrane transport of bivalent metal ions have been studied. Finally, applications in medicinal chemistry of such types of amino acids and peptides will be highlighted.},
keywords = {Amino Acids, Animals, Fullerenes, Humans, I2CT, Molecular Structure, Peptides, Solubility, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Marco Valeria De, Stier Gunter, Blandin Stephanie A, de Marco Ario
The solubility and stability of recombinant proteins are increased by their fusion to NusA Article de journal
Dans: Biochem. Biophys. Res. Commun., vol. 322, no. 3, p. 766–771, 2004, ISSN: 0006-291X.
Résumé | Liens | BibTeX | Étiquettes: blandin, Drug Stability, Escherichia coli Proteins, Genetic Vectors, Glutathione Transferase, Kinetics, M3i, Oxidation-Reduction, Peptide Elongation Factors, Recombinant Fusion Proteins, Recombinant Proteins, Solubility, Transcription Factors, Transcriptional Elongation Factors
@article{de_marco_solubility_2004,
title = {The solubility and stability of recombinant proteins are increased by their fusion to NusA},
author = {Valeria De Marco and Gunter Stier and Stephanie A Blandin and Ario de Marco},
doi = {10.1016/j.bbrc.2004.07.189},
issn = {0006-291X},
year = {2004},
date = {2004-01-01},
journal = {Biochem. Biophys. Res. Commun.},
volume = {322},
number = {3},
pages = {766--771},
abstract = {The new bacterial vector pETM60 enables the expression of His-tagged recombinant proteins fused to the C-terminus of NusA through a TEV protease recognition sequence. Three sequences coding for two protein domains (Xklp3A and Tep3Ag) and one membrane-bound viral protein (E8R) could not be expressed in a soluble form in bacteria. Their GST-fusions were mostly soluble but quickly degraded during purification. The same sequences cloned in pETM60 were efficiently purified by metal affinity and recovered soluble after the removal of the fusion partner. The NusA-fused constructs enabled to yield 13-20mg of fusion protein per litre of culture and 2.5-5mg of pure protein per litre of culture. Structural analysis indicated that the purified proteins were monodispersed and correctly folded. NusA has been used to raise antibodies that have been successfully used for Western blot and immunoprecipitation of NusA fusion proteins.},
keywords = {blandin, Drug Stability, Escherichia coli Proteins, Genetic Vectors, Glutathione Transferase, Kinetics, M3i, Oxidation-Reduction, Peptide Elongation Factors, Recombinant Fusion Proteins, Recombinant Proteins, Solubility, Transcription Factors, Transcriptional Elongation Factors},
pubstate = {published},
tppubtype = {article}
}
2002
Georgakilas Vasilios, Tagmatarchis Nikos, Pantarotto Davide, Bianco Alberto, Briand Jean-Paul, Prato Maurizio
Amino acid functionalisation of water soluble carbon nanotubes Article de journal
Dans: Chemical Communications (Cambridge, England), no. 24, p. 3050–3051, 2002, ISSN: 1359-7345.
Résumé | Liens | BibTeX | Étiquettes: Amino Acids, carbon, I2CT, Nanotubes, Solubility, Team-Bianco, water
@article{georgakilas_amino_2002,
title = {Amino acid functionalisation of water soluble carbon nanotubes},
author = {Vasilios Georgakilas and Nikos Tagmatarchis and Davide Pantarotto and Alberto Bianco and Jean-Paul Briand and Maurizio Prato},
doi = {10.1039/b209843a},
issn = {1359-7345},
year = {2002},
date = {2002-12-01},
journal = {Chemical Communications (Cambridge, England)},
number = {24},
pages = {3050--3051},
abstract = {High solubility of SWNTs and MWNTs in water is obtained by organic functionalisation; derivatisation with N-protected glycine is also easily achieved.},
keywords = {Amino Acids, carbon, I2CT, Nanotubes, Solubility, Team-Bianco, water},
pubstate = {published},
tppubtype = {article}
}