Génétique de la Réponse Immunitaire

@article{pmid34452932,

title = {Protein Phosphatase 4 Negatively Regulates the Immune Deficiency-NF-κB Pathway during the  Immune Response},

author = {Layale Salem Wehbe and Dana Barakat and Adrian Acker and Rita El Khoury and Jean-Marc Reichhart and Nicolas Matt and Laure El Chamy},

doi = {10.4049/jimmunol.1901497},

issn = {1550-6606},

year  = {2021},

date = {2021-08-27},

urldate = {2021-08-27},

journal = {J Immunol},

volume = {207},

number = {6},

pages = {1616--1626},

abstract = {The evolutionarily conserved immune deficiency (IMD) signaling pathway shields  against bacterial infections. It regulates the expression of antimicrobial peptides encoding genes through the activation of the NF-κB transcription factor Relish. Tight regulation of the signaling cascade ensures a balanced immune response, which is otherwise highly harmful. Several phosphorylation events mediate intracellular progression of the IMD pathway. However, signal termination by dephosphorylation remains largely elusive. Here, we identify the highly conserved protein phosphatase 4 (PP4) complex as a bona fide negative regulator of the IMD pathway. RNA interference-mediated gene silencing of , , and  which encode the catalytic and regulatory subunits of the phosphatase complex, respectively, caused a marked upregulation of bacterial-induced antimicrobial peptide gene expression in both  S2 cells and adult flies. Deregulated IMD signaling is associated with reduced lifespan of -deficient flies in the absence of any infection. In contrast, flies overexpressing this phosphatase are highly sensitive to bacterial infections. Altogether, our results highlight an evolutionarily conserved function of PP4c in the regulation of NF-κB signaling from  to mammals.},

keywords = {Drosophila, IKK complex, IMD, immune response, M3i, matt, NF-κB, PP4 complex},

pubstate = {published},

tppubtype = {article}

}