Wilhelm M., Wilhelm F. X.
Reverse transcription of retroviruses and LTR retrotransposons Article de journal
Dans: Cell Mol Life Sci, vol. 58, no. 9, p. 1246-62, 2001, (1420-682x Journal Article Review Review, Academic).
Résumé | BibTeX | Étiquettes: *Retroelements, *Terminal, Acid, Alignment, Amino, Animals, Base, Conformation, Data, DNA, Homology, Human, Molecular, Nucleic, Polymerase/*chemistry/*metabolism, Repeat, Retroviridae/*enzymology/*genetics, RNA-Directed, Sequence, Sequences
@article{,
title = {Reverse transcription of retroviruses and LTR retrotransposons},
author = { M. Wilhelm and F. X. Wilhelm},
year = {2001},
date = {2001-01-01},
journal = {Cell Mol Life Sci},
volume = {58},
number = {9},
pages = {1246-62},
abstract = {Retroelements are mobile genetic entities that replicate via reverse transcription of a template RNA. A key component to the life cycle of these elements is the enzyme reverse transcriptase (RT), which copies the single-stranded genomic RNA of the element into a linear double-stranded DNA that is ultimately integrated into the host genome by the element-encoded integrase. RT is a multifunctionnal enzyme which possesses RNA-dependent and DNA-dependent DNA polymerase activities as well as RNase H activity that specifically degrades the RNA strand of RNA-DNA duplexes. At some stages of the replication a strand-displacement activity of RT is also necessary. All activities are essential for the conversion of single-stranded genomic RNA into the double-stranded preintegrative DNA. This review focuses on the role of RT in the different steps of the replication process of retroelements. The features of retrotransposon replication which differ from the retroviral ones will be emphasized. In a second part of the review, the biochemical and enzymatic properties of two newly characterized retrotransposon RTs will be described. The role of the integrase domain in reverse transcriptase activity of some retroviral and retrotransposon RTs will be discussed.},
note = {1420-682x
Journal Article
Review
Review, Academic},
keywords = {*Retroelements, *Terminal, Acid, Alignment, Amino, Animals, Base, Conformation, Data, DNA, Homology, Human, Molecular, Nucleic, Polymerase/*chemistry/*metabolism, Repeat, Retroviridae/*enzymology/*genetics, RNA-Directed, Sequence, Sequences},
pubstate = {published},
tppubtype = {article}
}
Wilhelm M., Boutabout M., Heyman T., Wilhelm F. X.
Reverse transcription of the yeast Ty1 retrotransposon: the mode of first strand transfer is either intermolecular or intramolecular Article de journal
Dans: J Mol Biol, vol. 288, no. 4, p. 505-10, 1999, (0022-2836 Journal Article).
Résumé | BibTeX | Étiquettes: *Retroelements, *Transcription, Acid, Base, cerevisiae/*genetics, DNA, Genetic, Gov't, Non-U.S., Nucleic, Repetitive, Saccharomyces, Sequence, Sequences, Single-Stranded/genetics, Support
@article{,
title = {Reverse transcription of the yeast Ty1 retrotransposon: the mode of first strand transfer is either intermolecular or intramolecular},
author = { M. Wilhelm and M. Boutabout and T. Heyman and F. X. Wilhelm},
year = {1999},
date = {1999-01-01},
journal = {J Mol Biol},
volume = {288},
number = {4},
pages = {505-10},
abstract = {Replication of the yeast Ty1 retrotransposon occurs by a mechanism similar to that of retroviruses. According to the current model of retroviral reverse transcription, two strand transfers (the so-called minus-strand and plus-strand strong-stop DNA transfers) are required to produce full-length preintegrative DNA. Because two genomic RNA molecules are packaged inside the viral particles, the strand transfers can be either intra- or intermolecular. To study the mode of transfer of minus-strand strong-stop DNA during reverse transcription of the yeast Ty1 retrotransposon, we have analyzed the cDNA products that accumulate in the cytoplasmic virus-like particles of yeast cells harboring two marked Ty1 elements. Our results indicate that Ty1 minus-strand transfer occurs in a random manner with approximately similar frequencies of intra- and intermolecular transfer. It has been observed recently that intra- and intermolecular minus-strand transfer occur at similar frequencies during replication of a complex retrovirus such as HIV-1. These results together with the observation that genetic recombination occurs with a high frequency during minus-strand synthesis suggest that both packaged RNA molecules are needed for the synthesis of one minus-strand DNA.},
note = {0022-2836
Journal Article},
keywords = {*Retroelements, *Transcription, Acid, Base, cerevisiae/*genetics, DNA, Genetic, Gov't, Non-U.S., Nucleic, Repetitive, Saccharomyces, Sequence, Sequences, Single-Stranded/genetics, Support},
pubstate = {published},
tppubtype = {article}
}
Heyman T., Agoutin B., Friant S., Wilhelm F. X., Wilhelm M. L.
Plus-strand DNA synthesis of the yeast retrotransposon Ty1 is initiated at two sites, PPT1 next to the 3' LTR and PPT2 within the pol gene. PPT1 is sufficient for Ty1 transposition Article de journal
Dans: J Mol Biol, vol. 253, no. 2, p. 291-303, 1995, (0022-2836 Journal Article).
Résumé | BibTeX | Étiquettes: *DNA, *Genes, *Repetitive, *Retroelements, Acid, Base, C/analysis, cerevisiae/genetics/*virology, Chain, Cloning, Data, DNA, Fungal, Fungal/biosynthesis, Genes, Genetic, Genome, Gov't, Mapping, Molecular, Non-U.S., Nucleic, pol, Poly, Polymerase, Primers, Reaction, Replication, Restriction, Saccharomyces, Sequence, Sequences, Support, Transcription, Viral, Viral/*biosynthesis
@article{,
title = {Plus-strand DNA synthesis of the yeast retrotransposon Ty1 is initiated at two sites, PPT1 next to the 3' LTR and PPT2 within the pol gene. PPT1 is sufficient for Ty1 transposition},
author = { T. Heyman and B. Agoutin and S. Friant and F. X. Wilhelm and M. L. Wilhelm},
year = {1995},
date = {1995-01-01},
journal = {J Mol Biol},
volume = {253},
number = {2},
pages = {291-303},
abstract = {Long terminal repeat elements and retroviruses require primers for initiation of minus and plus-strand DNA synthesis by reverse transcriptase. Here we demonstrate genetically that plus-strand DNA synthesis of the yeast Ty1 element is initiated at two sites located at the 5' boundary of the 3' long terminal repeat (PPT1) and near the middle of the pol gene in the integrase coding sequence (PPT2). A consequence of the presence of two PPTs is that Ty1 plus-strand DNA exists as segments at some time during replication. Three fragments have been identified: the plus-strand strong-stop DNA initiated at PPT1, a downstream fragment initiated at PPT2 and an upstream fragment spanning the 5'-terminal part of Ty1 and a portion of the TyB gene. Characterization of the 3' ends of the plus-strand DNA fragments reveals (1) that the upstream fragment is elongated beyond PPT2 creating a plus-strand overlap and (2) that the majority of plus-strand strong-stop DNA fragments bear a copy of the minus-strand primer binding site in agreement with the accepted model of retroviral genomic RNA reverse transcription. The two polypurine tracts, PPT1 and PPT2, have an identical sequence GGGTGGTA. Mutations replacing purines by pyrimidines in this sequence significantly diminish or abolish initiation of plus-strand synthesis. Ty1 elements bearing a mutated PPT2 sequence are not defective for transposition whereas mutations in PPT1 abolish transposition.},
note = {0022-2836
Journal Article},
keywords = {*DNA, *Genes, *Repetitive, *Retroelements, Acid, Base, C/analysis, cerevisiae/genetics/*virology, Chain, Cloning, Data, DNA, Fungal, Fungal/biosynthesis, Genes, Genetic, Genome, Gov't, Mapping, Molecular, Non-U.S., Nucleic, pol, Poly, Polymerase, Primers, Reaction, Replication, Restriction, Saccharomyces, Sequence, Sequences, Support, Transcription, Viral, Viral/*biosynthesis},
pubstate = {published},
tppubtype = {article}
}