Popiela A., Gabrys M. S., Rabczynski J., Panszczyk M., Keith G., Baranowski W.
[Estimation of DNA methylation level in endometrial cancer tissues] Article de journal
Dans: Ginekol Pol, vol. 73, no. 11, p. 966-9, 2002, (0017-0011 Journal Article).
Résumé | BibTeX | Étiquettes: *DNA, 80, Abstract, Adenocarcinoma/chemistry/*genetics, Aged, and, Case-Control, Endometrial, English, Expression, Female, Gene, Human, Hyperplasia/genetics, Methylation, Middle, Neoplasms/chemistry/*genetics, Neoplastic, over, Regulation, Studies
@article{,
title = {[Estimation of DNA methylation level in endometrial cancer tissues]},
author = { A. Popiela and M. S. Gabrys and J. Rabczynski and M. Panszczyk and G. Keith and W. Baranowski},
year = {2002},
date = {2002-01-01},
journal = {Ginekol Pol},
volume = {73},
number = {11},
pages = {966-9},
abstract = {OBJECTIVES: A level of DNA methylation plays an important role in regulation of cellular gene's expression. Estimation of DNA methylation level in endometrial neoplastic tissues compared to normal endometrial samples was the aim of this study. DESIGN: It was to be shown, that changes in methylation rate in promotory regions could lead to carcinogenesis in particular cell. Authors describe an analysis of DNA methylation level in endometrial cancer tissues compared to DNA methylation level in normal or hyperplastic endometrium. MATERIAL AND METHODS: Endometrial samples from 88 women were collected. 56 of them were classified as adenocarcinoma, 20 as hyperplastic changes, 12 as normal endometrium-control group. DNA was isolated from tissues and than prepared to pm5dC and pdC. Than we performed a statistical analysis of results. RESULTS: The median DNA methylation level was significantly higher in neoplastic tissues than in normal endometrium. There was no difference between DNA methylation level between normal endometrium and hyperplastic changes. CONCLUSIONS: Authors conclude that neoplastic endometrial tissues show high DNA methylation rate compared to normal or hyperplastic endometrium.},
note = {0017-0011
Journal Article},
keywords = {*DNA, 80, Abstract, Adenocarcinoma/chemistry/*genetics, Aged, and, Case-Control, Endometrial, English, Expression, Female, Gene, Human, Hyperplasia/genetics, Methylation, Middle, Neoplasms/chemistry/*genetics, Neoplastic, over, Regulation, Studies},
pubstate = {published},
tppubtype = {article}
}
Popiela A., Gabrys M. S., Rabczynski J., Panszczyk M., Keith G., Baranowski W.
[Estimation of DNA methylation level in nonendometrial uterus malignancies] Article de journal
Dans: Ginekol Pol, vol. 73, no. 11, p. 962-5, 2002, (0017-0011 Journal Article).
Résumé | BibTeX | Étiquettes: *DNA, 80, Abstract, Age, Aged, and, Case-Control, English, Expression, Factors, Female, Gene, Human, Mesodermal/genetics/*metabolism, Methylation, Middle, Mixed, Neoplasms/genetics/*metabolism, Neoplastic, over, Regulation, silencing, Studies, tumor, Uterine
@article{,
title = {[Estimation of DNA methylation level in nonendometrial uterus malignancies]},
author = { A. Popiela and M. S. Gabrys and J. Rabczynski and M. Panszczyk and G. Keith and W. Baranowski},
year = {2002},
date = {2002-01-01},
journal = {Ginekol Pol},
volume = {73},
number = {11},
pages = {962-5},
abstract = {OBJECTIVES: Rebuilding of genome structure leads to many pathological states including neoplastic malignancies. Rebuilding often occurs as a process caused by disturbances in gene silencing mechanism. DNA methylation pattern is one of the most important mechanisms connected to gene's silencing. Estimation of DNA methylation level in nonendometrial uterine neoplastic tissues compared to normal endometrial samples was the aim of this study. DESIGN: It was to be shown, that changes in methylation rate in promotory regions could lead to carcinogenesis in particular cell. Authors describe an analysis of DNA methylation level in nonendometrial neoplastic uterine tissues compared to DNA methylation level in normal endometrium. MATERIALS AND METHODS: Tissue samples from 9 women with tumor mixtus mesodermalis were collected. 12 samples were normal endometrium-control group. DNA was isolated from tissues and than we performed an estimation of DNA methylation levels. Than we performed a statistical analysis of results. RESULTS: The median DNA methylation level was significantly higher in neoplastic tissues than in normal endometrium. CONCLUSIONS: Authors conclude, that DNA methylation level is higher in neoplastic tissues, but does not correlate with clinical stage of the disease.},
note = {0017-0011
Journal Article},
keywords = {*DNA, 80, Abstract, Age, Aged, and, Case-Control, English, Expression, Factors, Female, Gene, Human, Mesodermal/genetics/*metabolism, Methylation, Middle, Mixed, Neoplasms/genetics/*metabolism, Neoplastic, over, Regulation, silencing, Studies, tumor, Uterine},
pubstate = {published},
tppubtype = {article}
}
Postawski K., Olech-Fudali E., Korobowicz E., Jakowicki J. A., Keith G., Baranowski W.
[Hydrophobic DNA adducts in relationship to estrogen and progesterone receptors content in human uterine cancer.] Article de journal
Dans: Ginekol Pol, vol. 72, no. 9, p. 709-16, 2001, (0017-0011 Journal Article).
Résumé | BibTeX | Étiquettes: Abstract, Adducts/*analysis, Autoradiography, DNA, English, Estrogen/*analysis, Female, Human, Neoplasm/*analysis, Neoplasms/genetics/*pathology, Progesterone/*analysis, Receptors, Uterine
@article{,
title = {[Hydrophobic DNA adducts in relationship to estrogen and progesterone receptors content in human uterine cancer.]},
author = { K. Postawski and E. Olech-Fudali and E. Korobowicz and J. A. Jakowicki and G. Keith and W. Baranowski},
year = {2001},
date = {2001-01-01},
journal = {Ginekol Pol},
volume = {72},
number = {9},
pages = {709-16},
abstract = {OBJECTIVE: Determination of the relationship between hydrophobic DNA adducts (A) and estrogen receptors (ER) and progesterone (PR) receptor status in uterine cancers. METHODS: Using the P1 enriched version of 32P-postlabeling for hydrophobic DNA adducts detection on polyethyleneimine (PEI) cellulose thin layer chromatograms (TLC) we examined 11 uterine cancer DNAs. The quantification of the adducts was performed by Cerenkov counting of the spots. ER and PR status was recognized histochemically and H-score estimate was performed for each investigated cancer tissue. Patterns of uterine cancer DNA adducts were compared to the maps of adducts recognized in normal human endometrium. RESULTS: In three of the studied uterine cancers there was no positive staining of ER and PR; in one case there was a weak ER staining but PR staining was negative. In ER negative tumors the A level was significantly higher than in ER positive cancers (138.1 +/- 64.1 vs. 49.7 +/- 26.8 adducts per 10(9) nucleotides, respectively, p < 0.05). Highest A levels were found in two ER and PR negative G3 metastatic tumors. Finally, in all investigated cancers there was a strong, inverse correlation between ER content and A level (r = -0.67, p < 0.03). In addition, the correlation between PR level and A was of borderline significance (r = -0.6},
note = {0017-0011
Journal Article},
keywords = {Abstract, Adducts/*analysis, Autoradiography, DNA, English, Estrogen/*analysis, Female, Human, Neoplasm/*analysis, Neoplasms/genetics/*pathology, Progesterone/*analysis, Receptors, Uterine},
pubstate = {published},
tppubtype = {article}
}
Postawski K., Olech-Fudali E., Jakowicki J. A., Korobowicz E., Keith G., Baranowski W.
[Overall genomic DNA methylation in relation to estrogen] Article de journal
Dans: Ginekol Pol, vol. 71, no. 9, p. 1206-11, 2000, (0017-0011 Journal Article).
Résumé | BibTeX | Étiquettes: 80, Abstract, Aged, and, Biopsy, DNA, English, Estrogen/*metabolism, Female, Gov't, Human, Methylases/metabolism, Methylation, Middle, modification, Neoplasms/*metabolism/*pathology, Non-U.S., over, Progesterone/*metabolism, Receptors, Support, Uterine, Uterus/*metabolism/pathology
@article{,
title = {[Overall genomic DNA methylation in relation to estrogen]},
author = { K. Postawski and E. Olech-Fudali and J. A. Jakowicki and E. Korobowicz and G. Keith and W. Baranowski},
year = {2000},
date = {2000-01-01},
journal = {Ginekol Pol},
volume = {71},
number = {9},
pages = {1206-11},
abstract = {Overall genomic DNA methylation was analyzed using enzymatic digestion into nucleotides, 32P postlabeling, two-dimensional thin-layer chromatography on cellulose plates and phosphobioimaging quantitation, in relation to immunohistochemically measured estrogen (ER) and progesterone receptor (PR) status of 15 uterine cancers. Mean 5-methyldeoxycytosine (m5dC) content did not differ between ER-positive and ER-negative neoplasms. Highest values of m5dC were noted both in ER-negative and ER-positive tumors. Additionally, there was no low DNA methylation in ER negative uterine cancer tissues. Decrease of the overall genomic DNA methylation could be related to the increase of ER/PR ratio, however it was not significant in our investigation. The potential role of steroid receptors status in uterine cancer tissue is discussed.},
note = {0017-0011
Journal Article},
keywords = {80, Abstract, Aged, and, Biopsy, DNA, English, Estrogen/*metabolism, Female, Gov't, Human, Methylases/metabolism, Methylation, Middle, modification, Neoplasms/*metabolism/*pathology, Non-U.S., over, Progesterone/*metabolism, Receptors, Support, Uterine, Uterus/*metabolism/pathology},
pubstate = {published},
tppubtype = {article}
}
Baranowski W., Tomaszewski J., Keith G.
[Methylation of DNA in tissue of ovarian malignant tumors in women] Article de journal
Dans: Ginekol Pol, vol. 64, no. 4, p. 169-73, 1993, (0017-0011 Journal Article).
Résumé | BibTeX | Étiquettes: Abstract, Adult, Aged, Carcinoma/genetics, Cell, DNA, English, Female, Human, Methylation, Middle, Neoplasm/*metabolism, Neoplasms/*genetics, Ovarian, Sertoli, Tumor/genetics
@article{,
title = {[Methylation of DNA in tissue of ovarian malignant tumors in women]},
author = { W. Baranowski and J. Tomaszewski and G. Keith},
year = {1993},
date = {1993-01-01},
journal = {Ginekol Pol},
volume = {64},
number = {4},
pages = {169-73},
abstract = {DNA methylation level from woman ovarian malignant tissues was investigated by 32P postlabeling of the single nucleotides, separation on TLC with followed autoradiography and Cerenkov counting. Slight differences in DNA methylation extent were found in malignant ovarian tumors as compared to normal myometrium and benign uterine tumor [myomas]. Lowest level of m5dC in relation to C and also in relation to total DNA was found in carcinoma embryonal tissue whereas maximal DNA methylation level was obtained in folliculoma tissue. This preliminary report needs further investigation of particularly genes methylation.},
note = {0017-0011
Journal Article},
keywords = {Abstract, Adult, Aged, Carcinoma/genetics, Cell, DNA, English, Female, Human, Methylation, Middle, Neoplasm/*metabolism, Neoplasms/*genetics, Ovarian, Sertoli, Tumor/genetics},
pubstate = {published},
tppubtype = {article}
}