I2CT

@article{nokey,

title = {The virus-induced cyclic dinucleotide 2'3'-c-di-GMP mediates STING-dependent antiviral immunity in Drosophila},

author = {Cai H and Li L and Slavik KM and Huang J and Yin T and Ai X and Hédelin L and Haas G and Xiang Z and Yang Y and Li X and Chen Y and Wei Z and Deng H and Chen D and Jiao R and Martins N and Meignin C and Kranzusch PJ and Imler JL},

editor = {Elsevier Inc. },

url = {https://pubmed.ncbi.nlm.nih.gov/37659413/},

doi = {10.1016/j.immuni.2023.08.006 },

year  = {2023},

date = {2023-09-12},

urldate = {2023-09-12},

journal = {Immunity},

volume = {56},

issue = {9},

pages = {1991-2005},

abstract = {In mammals, the enzyme cGAS senses the presence of cytosolic DNA and synthesizes the cyclic dinucleotide (CDN) 2'3'-cGAMP, which triggers STING-dependent immunity. In Drosophila melanogaster, two cGAS-like receptors (cGLRs) produce 3'2'-cGAMP and 2'3'-cGAMP to activate STING. We explored CDN-mediated immunity in 14 Drosophila species covering 50 million years of evolution and found that 2'3'-cGAMP and 3'2'-cGAMP failed to control infection by Drosophila C virus in D. serrata and two other species. We discovered diverse CDNs produced in a cGLR-dependent manner in response to viral infection in D. melanogaster, including 2'3'-c-di-GMP. This CDN was a more potent STING agonist than cGAMP in D. melanogaster and it also activated a strong antiviral transcriptional response in D. serrata. Our results shed light on the evolution of cGLRs in flies and provide a basis for understanding the function and regulation of this emerging family of pattern recognition receptors in animal innate immunity. },

keywords = {c-di-GMP, cGAMP, cGAS, cGLR, cyclic dinucleotide, Drosophila, Evolution, Hua, imler, M3i, meignin, pattern recognition receptor, STING, virus},

pubstate = {published},

tppubtype = {article}

}

@article{dedaMarques2022,

title = {Invading viral DNA triggers dsRNA synthesis by RNA polymerase II to activate antiviral RNA interference in Drosophila},

author = {Isaque J.S. de Faria and Eric R.G.R. Aguiar and Roenick P. Olmo and Juliana Alves da Silva and Laurent Daeffler and Richard W. Carthew and Jean-Luc Imler and Joao T. Marques},

doi = {10.1016/j.celrep.2022.110976},

year  = {2022},

date = {2022-06-21},

urldate = {2022-06-21},

journal = {Cell Reports},

volume = {39},

pages = {110976},

abstract = {dsRNA sensing triggers antiviral responses against RNA and DNA viruses in diverse eukaryotes. In Drosophila, Invertebrate iridescent virus 6 (IIV-6), a large DNA virus, triggers production of small interfering RNAs (siRNAs) by the dsRNA sensor Dicer-2. Here, we show that host RNA polymerase II (RNAPII) bidirec- tionally transcribes specific AT-rich regions of the IIV-6 DNA genome to generate dsRNA. Both replicative and naked IIV-6 genomes trigger production of dsRNA in Drosophila cells, implying direct sensing of invading DNA. Loquacious-PD, a Dicer-2 co-factor essential for the biogenesis of endogenous siRNAs, is dispensable for processing of IIV-6-derived dsRNAs, which suggests that they are distinct. Consistent with this finding, inhibition of the RNAPII co-factor P-TEFb affects the synthesis of endogenous, but not virus-derived, dsRNA. Altogether, our results suggest that a non-canonical RNAPII complex recognizes invading viral DNA to synthesize virus-derived dsRNA, which activates the antiviral siRNA pathway in Drosophila.},

keywords = {antiviral, Drosophila, dsRNA, imler, M3i, Marques, protocol, RNA Interference},

pubstate = {published},

tppubtype = {article}

}

@article{Imler2022,

title = {cGAS-like receptor-mediated immunity: the insect perspective},

author = {H Cai and C Meignin and JL Imler},

doi = {10.1016/j.coi.2022.01.005},

year  = {2022},

date = {2022-02-08},

urldate = {2022-02-08},

journal = {Current Opinion in Immunology},

volume = {74},

pages = {183-189},

abstract = {The cGAS-STING pathway plays a central role in the detection of DNA in the cytosol of mammalian cells and activation of immunity. Although the early evolutionary origin of this pathway in animals has been noted, its ancestral functions have remained elusive so far. We review here new findings in invertebrates establishing a role in sensing and signaling infection, triggering potent transcriptional responses, in addition to autophagy. Results from flies and moths/butterflies points to the importance of STING signaling in antiviral immunity in insects. The recent characterization of cGAS-like receptors in Drosophila reveals the plasticity of this family of pattern-recognition receptors, able to accommodate ligands different from DNA and to produce cyclic dinucleotides beyond 2′3′-cGAMP.},

keywords = {imler, M3i, meignin},

pubstate = {published},

tppubtype = {article}

}

@article{pmid34853303,

title = {Cross-species analysis of viral nucleic acid interacting proteins identifies TAOKs as innate immune regulators},

author = {Friederike L Pennemann and Assel Mussabekova and Christian Urban and Alexey Stukalov and Line Lykke Andersen and Vincent Grass and Teresa Maria Lavacca and Cathleen Holze and Lila Oubraham and Yasmine Benamrouche and Enrico Girardi and Rasha E Boulos and Rune Hartmann and Giulio Superti-Furga and Matthias Habjan and Jean-Luc Imler and Carine Meignin and Andreas Pichlmair},

doi = {10.1038/s41467-021-27192-w},

issn = {2041-1723},

year  = {2021},

date = {2021-12-01},

urldate = {2021-12-01},

journal = {Nat Commun},

volume = {12},

number = {1},

pages = {7009},

abstract = {The cell intrinsic antiviral response of multicellular organisms developed over millions of years and critically relies on the ability to sense and eliminate viral nucleic acids. Here we use an affinity proteomics approach in evolutionary distant species (human, mouse and fly) to identify proteins that are conserved in their ability to associate with diverse viral nucleic acids. This approach shows a core of orthologous proteins targeting viral genetic material and species-specific interactions. Functional characterization of the influence of 181 candidates on replication of 6 distinct viruses in human cells and flies identifies 128 nucleic acid binding proteins with an impact on virus growth. We identify the family of TAO kinases (TAOK1, -2 and -3) as dsRNA-interacting antiviral proteins and show their requirement for type-I interferon induction. Depletion of TAO kinases in mammals or flies leads to an impaired response to virus infection characterized by a reduced induction of interferon stimulated genes in mammals and impaired expression of srg1 and diedel in flies. Overall, our study shows a larger set of proteins able to mediate the interaction between viral genetic material and host factors than anticipated so far, attesting to the ancestral roots of innate immunity and to the lineage-specific pressures exerted by viruses.},

keywords = {antiviral innate immunity, imler, M3i, meignin},

pubstate = {published},

tppubtype = {article}

}

@article{Hartmann2021,

title = {Two cGAS-like receptors induce antiviral immunity in Drosophila},

author = {Andreas Holleufer AND Kasper Grønbjerg Winther AND Hans Henrik Gad AND Xianlong Ai AND Yuqiang Chen AND Lihua Li AND Ziming Wei AND Huimin Deng AND Jiyong Liu AND Ninna Ahlmann Frederiksen AND Bine Simonsen AND Line Lykke Andersen AND Karin Kleigrewe AND Louise Dalskov AND Andreas Pichlmair AND Hua Cai AND Jean-Luc Imler AND Rune Hartmann},

editor = {Nature Publishing Group},

doi = {https://doi.org/10.1038/s41586-021-03800-z},

year  = {2021},

date = {2021-07-14},

journal = {Nature},

volume = {597},

pages = {114-118},

abstract = {In mammals, cyclic GMP-AMP (cGAMP) synthase (cGAS) produces the cyclic dinucleotide (CDN) 2'3'-cGAMP in response to cytosolic DNA and this triggers an antiviral immune response. cGAS belongs to a large family of cGAS/DncV-like nucleotidyltransferases, present in both prokaryotes1 and eukaryotes2–5. In bacteria, these enzymes synthesize a range of cyclic oligonucleotide and have recently emerged as important regulators of phage infections6–8. Here, we identify two novel cGAS-like receptors (cGLRs) in the insect Drosophila melanogaster. We show that cGLR1 and cGLR2 activate Sting and NF-κB dependent antiviral immunity in response to infection with RNA or DNA viruses. cGLR1 is activated by dsRNA to produce the novel CDN 3'2'-cGAMP whereas cGLR2 produces a combination of 2'3'-cGAMP and 3'2' cGAMP in response to a yet unidentified stimulus. Our data establish cGAS as the founding member of a family of receptors sensing different types of nucleic acids and triggering immunity through production of CDNs beyond 2'3'-cGAMP.},

keywords = {antiviral immunity, cGAS-like receptors, Drosophila, imler, M3i},

pubstate = {published},

tppubtype = {article}

}

@article{pmid34261127,

title = {cGAS-like receptors sense RNA and control 3'2'-cGAMP signalling in Drosophila},

author = {Kailey M Slavik and Benjamin R Morehouse and Adelyn E Ragucci and Wen Zhou and Xianlong Ai and Yuqiang Chen and Lihua Li and Ziming Wei and Heike Bähre and Martin König and Roland Seifert and Amy S Y Lee and Hua Cai and Jean-Luc Imler and Philip J Kranzusch},

doi = {10.1038/s41586-021-03743-5},

issn = {1476-4687},

year  = {2021},

date = {2021-07-14},

journal = {Nature},

volume = {597},

number = {7874},

pages = {109--113},

abstract = {Cyclic GMP-AMP synthase (cGAS) is a cytosolic DNA sensor that produces the second messenger cG[2'-5']pA[3'-5']p (2'3'-cGAMP) and controls activation of innate immunity in mammalian cells. Animal genomes typically encode multiple proteins with predicted homology to cGAS, but the function of these uncharacterized enzymes is unknown. Here we show that cGAS-like receptors (cGLRs) are innate immune sensors that are capable of recognizing divergent molecular patterns and catalysing synthesis of distinct nucleotide second messenger signals. Crystal structures of human and insect cGLRs reveal a nucleotidyltransferase signalling core shared with cGAS and a diversified primary ligand-binding surface modified with notable insertions and deletions. We demonstrate that surface remodelling of cGLRs enables altered ligand specificity and used a forward biochemical screen to identify cGLR1 as a double-stranded RNA sensor in the model organism Drosophila melanogaster. We show that RNA recognition activates Drosophila cGLR1 to synthesize the novel product cG[3'-5']pA[2'-5']p (3'2'-cGAMP). A crystal structure of Drosophila stimulator of interferon genes (dSTING) in complex with 3'2'-cGAMP explains selective isomer recognition, and 3'2'-cGAMP induces an enhanced antiviral state in vivo that protects from viral infection. Similar to radiation of Toll-like receptors in pathogen immunity, our results establish cGLRs as a diverse family of metazoan pattern recognition receptors.},

keywords = {imler, M3i},

pubstate = {published},

tppubtype = {article}

}

@article{Cai2021,

title = {cGAS-STING: insight on the evolution of a primordial antiviral signaling cassette},

author = {Hua Cai and Jean-Luc Imler

},

url = { https://doi.org/10.12703/r/10-54},

doi = {10.12703/r/10-54},

year  = {2021},

date = {2021-06-08},

journal = {Faculty Reviews},

volume = {10},

pages = {54},

abstract = {Stimulator of interferon genes (STING) functions in the cytosolic DNA-sensing pathway of innate immunity in mammals. It is activated upon binding the cyclic dinucleotide 2′3′-cGAMP, a second messenger produced by the enzyme cyclic guanosine monophosphate–adenosine monophosphate synthase (cGAS), which acts as the receptor for DNA in this pathway, and triggers the expression of interferons and other viral stress-induced genes. The ancient origin of STING in the evolution of animals had been noted, but its primitive function was speculative. We review here recent advances in the remarkable history of cGAS-STING signaling, which establish that cGAS is a member of the family of cGAS/DncV-like nucleotidyltransferases (CD-NTases). In bacteria, CD-NTases synthesize a wide range of cyclic oligonucleotide second messengers in response to bacteriophage infections, which in turn activate a variety of effector proteins to abort phage infection. Among these effectors, some are related to STING, revealing an ancestral function for the cGAS-STING cassette in antiviral host defense. Study of STING signaling in invertebrate animals is consistent with an early acquisition in the history of metazoans of CD-NTase- and STING-encoding genes to counter the universal threat of viruses. In particular, STING-dependent immunity appears to play a previously unsuspected important role in some insects. These discoveries open up interesting perspectives for the use of model organisms to decipher emerging aspects of cGAS-STING biology in mammals, such as the activation of interferon-independent responses or the function and regulation of cGAS in the nucleus.},

keywords = {imler, M3i},

pubstate = {published},

tppubtype = {article}

}

@article{Leite2021,

title = {Distinct Roles of Hemocytes at Different Stages of Infection by Dengue and Zika Viruses in Aedes aegypti Mosquitoes},

author = {Thiago H J F Leite and Alvaro G A Ferreira and Jean-Luc Imler and João T Marques},

url = {https://www.frontiersin.org/articles/10.3389/fimmu.2021.660873/full},

doi = {10.3389/fimmu.2021.660873},

year  = {2021},

date = {2021-05-13},

journal = {Frontiers in immunology},

volume = {12},

pages = {660873},

abstract = {Aedes aegypti mosquitoes are vectors for arboviruses of medical importance such as dengue (DENV) and Zika (ZIKV) viruses. Different innate immune pathways contribute to the control of arboviruses in the mosquito vector including RNA interference, Toll and Jak- STAT pathways. However, the role of cellular responses mediated by circulating macrophage-like cells known as hemocytes remains unclear. Here we show that hemocytes are recruited to the midgut of Ae. aegypti mosquitoes in response to DENV or ZIKV. Blockade of the phagocytic function of hemocytes using latex beads induced increased accumulation of hemocytes in the midgut and a reduction in virus infection levels in this organ. In contrast, inhibition of phagocytosis by hemocytes led to increased systemic dissemination and replication of DENV and ZIKV. Hence, our work reveals a dual role for hemocytes in Ae. aegypti mosquitoes, whereby phagocytosis is not required to control viral infection in the midgut but is essential to restrict systemic dissemination. Further understanding of the mechanism behind this duality could help the design of vector-based strategies to prevent transmission of arboviruses.},

keywords = {Aedes, Dengue, Hemocytes, imler, innate immunity, M3i, Marques, Zika},

pubstate = {published},

tppubtype = {article}

}

@article{H2021,

title = {Insect decline: immediate action is needed},

author = {H Jactel and JL Imler and L Lambrechts and AB Failloux and JD Lebreton and Y Le Maho and JC Duplessy and P Cossart and P Grandcolas},

url = {https://doi.org/10.5802/crbiol.37},

doi = {10.5802/crbiol.37},

issn = {17683238},

year  = {2021},

date = {2021-01-25},

journal = {Comptes Rendus. Biologies},

abstract = { Insects appeared more than 400 million years ago and they represent the richest and most diverse taxonomic group with several million species. Yet, under the combined effect of the loss of natural habitats, the intensification of agriculture with massive use of pesticides, global warming and biological invasions, insects show alarming signs of decline. Although difficult to quantify, species extinction and population reductions are confirmed for many ecosystems. This results in a loss of services such as the pollination of plants, including food crops, the recycling of organic matter, the supply of goods such as honey and the stability of food webs. It is therefore urgent to halt the decline of Insects. We recommend implementing long-term monitoring of populations, tackling the causes of insect decline by reducing the use of synthetic insecticides, preserving natural habitats, and reinventing a positive relationship between humans and insects.



Apparus il y a plus de 400 millions d’années, les Insectes représentent le groupe taxonomique le plus riche et diversifié, avec plusieurs millions d’espèces. Sous l’effet de la disparition des habitats, de l’intensification de l’agriculture avec l’usage massif des pesticides, du réchauffement climatique et des invasions biologiques, les Insectes montrent des signes alarmants de déclin. Bien que difficiles à quantifier, la disparition des espèces et la réduction de leurs populations sont avérées et communes à de nombreux écosystèmes. Elles se traduisent par une perte des services rendus, comme la pollinisa- tion des plantes vivrières, le recyclage de la matière organique, la fourniture de biens comme le miel, et l’équilibre des réseaux trophiques. Il est donc urgent de freiner le déclin des Insectes. Pour cela, il faut mettre en œuvre des suivis à long terme des populations, réduire l’usage des insecticides de syn- thèse, préserver les habitats naturels, et réinventer la relation de l’Homme à l’Insecte en revalorisant son image et ses usages.},

keywords = {imler, M3i},

pubstate = {published},

tppubtype = {article}

}

@article{Schneider2020,

title = {Sensing and signalling viral infection in drosophila},

author = {J Schneider and JL Imler},

url = {https://doi.org/10.1016/j.dci.2020.103985

},

doi = {10.1016/j.dci.2020.103985},

year  = {2020},

date = {2020-12-23},

journal = {Developmental and Comparative Immunology},

volume = {117},

abstract = {The fruitfly Drosophila melanogaster is a valuable model to unravel mechanisms of innate immunity, in particular in the context of viral infections. RNA interference, and more specifically the small interfering RNA pathway, is a major component of antiviral immunity in drosophila. In addition, the contribution of inducible transcriptional responses to the control of viruses in drosophila and other invertebrates is increasingly recognized. In particular, the recent discovery of a STING-IKKβ-Relish signalling cassette in drosophila has confirmed that NF-κB tran- scription factors play an important role in the control of viral infections, in addition to bacterial and fungal infections. Here, we review recent developments in the field, which begin to shed light on the mechanisms involved in sensing of viral infections and in signalling leading to production of antiviral effectors.},

keywords = {imler, M3i},

pubstate = {published},

tppubtype = {article}

}

@article{Cai_2020,

title = {2'3'-cGAMP triggers a STING- and NF-κB-dependent broad antiviral response in Drosophila},

author = {H Cai and A Holleufer and B Simonsen and J Schneider and A Lemoine and HH Gad and J Huang and J Huang and D Chen and T Peng and JT Marques and R Hartmann and N Martins and JL Imler},

url = {https://pubmed.ncbi.nlm.nih.gov/33262294/},

doi = {10.1126/scisignal.abc4537 },

year  = {2020},

date = {2020-12-01},

journal = {Science Signaling},

volume = {13},

number = {660},

pages = {eabc4537},

abstract = {We previously reported that an ortholog of STING regulates infection by picorna-like viruses in Drosophila In mammals, STING is activated by the cyclic dinucleotide 2'3'-cGAMP produced by cGAS, which acts as a receptor for cytosolic DNA. Here, we showed that injection of flies with 2'3'-cGAMP induced the expression of dSTING-regulated genes. Coinjection of 2'3'-cGAMP with a panel of RNA or DNA viruses resulted in substantially reduced viral replication. This 2'3'-cGAMP-mediated protection was still observed in flies with mutations in Atg7 and AGO2, genes that encode key components of the autophagy and small interfering RNA pathways, respectively. By contrast, this protection was abrogated in flies with mutations in the gene encoding the NF-κB transcription factor Relish. Transcriptomic analysis of 2'3'-cGAMP-injected flies revealed a complex response pattern in which genes were rapidly induced, induced after a delay, or induced in a sustained manner. Our results reveal that dSTING regulates an NF-κB-dependent antiviral program that predates the emergence of interferons in vertebrates. },

keywords = {imler, M3i, Marques, STING},

pubstate = {published},

tppubtype = {article}

}

@article{Aguiar_2020,

title = {A single unidirectional piRNA cluster similar to the flamenco locus is the major source of EVE-derived transcription and small RNAs in Aedes aegypti mosquitoes },

author = {ERGR Aguiar and JPP de Almeida and LR Queiroz and LS Oliveira and RP Olmo and IJDS de Faria and JL Imler and A Gruber and BJ Matthews and JT Marques},

url = {https://rnajournal.cshlp.org/content/26/5/581.long},

doi = {10.1261/rna.073965.119},

year  = {2020},

date = {2020-01-29},

journal = {RNA},

volume = {26},

number = {5},

pages = {581-594},

abstract = {Endogenous viral elements (EVEs) are found in many eukaryotic genomes. Despite considerable knowledge about genomic elements such as transposons (TEs) and retroviruses, we still lack information about nonretroviral EVEs. Aedes aegypti mosquitoes have a highly repetitive genome that is covered with EVEs. Here, we identified 129 nonretroviral EVEs in the AaegL5 version of the A. aegypti genome. These EVEs were significantly associated with TEs and preferentially located in repeat-rich clusters within intergenic regions. Genome-wide transcriptome analysis showed that most EVEs generated transcripts although only around 1.4% were sense RNAs. The majority of EVE transcription was antisense and correlated with the generation of EVE-derived small RNAs. A single genomic cluster of EVEs located in a 143 kb repetitive region in chromosome 2 contributed with 42% of antisense transcription and 45% of small RNAs derived from viral elements. This region was enriched for TE-EVE hybrids organized in the same coding strand. These generated a single long antisense transcript that correlated with the generation of phased primary PIWI-interacting RNAs (piRNAs). The putative promoter of this region had a conserved binding site for the transcription factor Cubitus interruptus, a key regulator of the flamenco locus in Drosophila melanogaster Here, we have identified a single unidirectional piRNA cluster in the A. aegypti genome that is the major source of EVE transcription fueling the generation of antisense small RNAs in mosquitoes. We propose that this region is a flamenco-like locus in A. aegypti due to its relatedness to the major unidirectional piRNA cluster in Drosophila melanogaster. },

keywords = {A. aegypti, Aedes aegypti, endogenous viral elements, EVE, flamenco locus, imler, M3i, Marques, piRNA, piRNAs, RNA Interference},

pubstate = {published},

tppubtype = {article}

}

@article{goto2020,

title = {The Kinase IKKβ Regulates a STING-and NF-κB-Dependent Antiviral Response Pathway in Drosophila},

author = {Akira Goto and Kiyoshi Okado and Nelson Martins and Hua Cai and Vincent Barbier and Olivier Lamiable and Laurent Troxler and Estelle Santiago and Lauriane Kuhn and Donggi Paik and Neal Silverman and Andreas Holleufer and Rune Hartmann and Jiyong Liu and Tao Peng and Jules A Hoffmann and Carine Meignin and Laurent Daeffler and Jean-Luc Imler

},

url = {https://www-sciencedirect-com.insb.bib.cnrs.fr/science/article/pii/S107476131930528X},

doi = {10.1016/j.immuni.2019.12.009 },

year  = {2020},

date = {2020-01-14},

journal = {Immunity},

volume = {52},

number = {1},

pages = {200},

abstract = {Antiviral immunity inDrosophilainvolves RNA inter-ference and poorly characterized inducible re-sponses. Here, we showed that two components ofthe IMD pathway, the kinase dIKKband the tran-scription factor Relish, were required to controlinfection by two picorna-like viruses. We identifieda set of genes induced by viral infection and regu-lated by dIKKband Relish, which included an ortho-log of STING. We showed that dSTING participatedin the control of infection by picorna-like viruses,acting upstream of dIKKbto regulate expression ofNazo, an antiviral factor. Our data reveal an antiviralfunction for STING in an animal model devoid of inter-ferons and suggest an evolutionarily ancient role forthis molecule in antiviral immunity.},

keywords = {antiviral, Drosophila, hoffmann, imler, Kinase, M3i, meignin, STING},

pubstate = {published},

tppubtype = {article}

}

@article{Talide_2020,

title = {Sensing Viral Infections in Insects: A Dearth of Pathway Receptors},

author = {L Talide and JL Imler and C Meignin},

url = {https://www.caister.com/insectvirology2},

doi = {10.21775/cimb.034.031},

isbn = {9781912530090},

year  = {2019},

date = {2019-06-06},

journal = {Curr Issues Mol Biol},

volume = {34},

pages = {31-60},

abstract = {Insects, the most diverse group of animals, can be infected by an extraordinary diversity of viruses. Among them, arthropod-borne viruses can be transmitted to humans, while bee and silkworm viruses cause important economic losses. Like all invertebrates, insects rely solely on innate immunity to counter viral infections. Protein-based mechanisms, involving restriction factors and evolutionarily conserved signaling pathways regulating transcription factors of the NF-kB and STAT families, participate in the control of viral infections in insects. In addition, RNA-based responses play a major role in the silencing of viral RNAs. We review here our current state of knowledge on insect antiviral defense mechanisms, which include conserved as well as adaptive, insect-specific strategies. Identification of the innate immunity receptors that sense viral infection in insects remains a major challenge for the field. },

keywords = {imler, innate immunity, M3i, meignin, STAT, viral Infection},

pubstate = {published},

tppubtype = {article}

}

@article{Olmo_2019,

title = {The insect reservoir of biodiversity for viruses and for antiviral mechanisms},

author = {RP Olmo and NE Martins and ERGR Aguiar and JT Marques and JL Imler},

url = {https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000600604&lng=en&nrm=iso&tlng=en},

doi = {10.1590/0001-3765201920190122},

year  = {2019},

date = {2019-06-03},

journal = { An Acad Bras Cienc },

volume = {91},

number = {Suppl 3},

pages = {e20190122},

abstract = {Insects are the most diverse group of animals. They can be infected by an extraordinary diversity of viruses. Among them, arthropod-borne viruses (arboviruses) can be transmitted to humans. High-throughput sequencing of small RNAs from insects provides insight into their virome, which may help understand the dynamics of vector borne infectious diseases. Furthermore, investigating the mechanisms that restrict viral infections in insects points to genetic innovations that may inspire novel antiviral strategies. },

keywords = {antiviral immunity, imler, insects, M3i, Marques, metagenomics, restriction factors, RNA Interference, virome},

pubstate = {published},

tppubtype = {article}

}

@article{Martins_2019jbio,

title = {Les insectes : un fantastique réservoir de virus et de gènes antiviraux},

author = {NE Martins and RP Olmo and ERGR Aguiar and JT Marques and JL Imler},

url = {https://www.biologie-journal.org/articles/jbio/abs/2018/02/jbio190008/jbio190008.html},

doi = {10.1051/jbio/2019008},

year  = {2019},

date = {2019-04-11},

journal = {Biologie Aujourd'hui},

volume = {212},

number = {3-4},

pages = {101-106},

abstract = {Les insectes forment le groupe d’animaux qui présente la plus grande diversité. Des travaux récents de métagénomique montrent qu’ils peuvent être infectés par une diversité extraordinaire de virus. Parmi eux, les arbovirus (arthropod-borne viruses) peuvent être transmis à l’Homme par les insectes hématophages, notamment les moustiques. Le séquençage à haut débit des petits ARN des insectes fournit des informations sur leur virome, un paramètre qui pourrait contribuer à expliquer la dynamique de la transmission des maladies infectieuses par des insectes vecteurs. D’autre part, la caractérisation des mécanismes qui restreignent les infections virales chez les insectes révèle des innovations génétiques qui pourraient à terme inspirer de nouvelles stratégies antivirales.},

keywords = {antiviral immunity, ARN interference, imler, Insect, M3i, Marques, metagenomic, virome},

pubstate = {published},

tppubtype = {article}

}

@article{Martins_2019i,

title = {A Transgenic Flock House Virus Replicon Reveals an RNAi Independent Antiviral Mechanism Acting in Drosophila Follicular Somatic Cells },

author = {N Martins and A Lemoine and E Santiago and S Paro and JL Imler and C Meignin},

url = {https://www.g3journal.org/content/9/2/403.long},

doi = {10.1534/g3.118.200872},

year  = {2019},

date = {2019-02-07},

journal = { G3 (Bethesda)},

volume = {9},

number = {2},

pages = {403-412},

keywords = {antiviral immunity, Drosophila melanogaster, Follicular somatic cells, Genetics of Immunity, imler, M3i, meignin, replicon, Viral replicon},

pubstate = {published},

tppubtype = {article}

}

@article{Olmo_2018,

title = {Control of dengue virus in the midgut of Aedes aegypti by ectopic expression of the dsRNA-binding protein Loqs2 },

author = {RP Olmo and AGA Ferreira and TC Izidoro-Toledo and ERGR Aguiar and IJS de Faria and KPR de Souza and KP Osório and L Kuhn and P Hammann and EG de Andrade and YM Todjro and MN Rocha and THJF Leite and SCG Amadou and JN Armache and S Paro and CD de Oliveira and FD Carvalho and LA Moreira and E Marois and JL Imler and JT Marques},

url = {https://www.nature.com/articles/s41564-018-0268-6},

doi = {10.1038/s41564-018-0268-6},

year  = {2018},

date = {2018-10-29},

journal = {Nature Microbiology},

volume = {3},

number = {12},

pages = {1385-1393},

abstract = {Dengue virus (DENV) is an arbovirus transmitted to humans by Aedes mosquitoes. In the insect vector, the small interfering RNA (siRNA) pathway is an important antiviral mechanism against DENV. However, it remains unclear when and where the siRNA pathway acts during the virus cycle. Here, we show that the siRNA pathway fails to efficiently silence DENV in the midgut of Aedes aegypti although it is essential to restrict systemic replication. Accumulation of DENV-derived siRNAs in the midgut reveals that impaired silencing results from a defect downstream of small RNA biogenesis. Notably, silencing triggered by endogenous and exogenous dsRNAs remained effective in the midgut where known components of the siRNA pathway, including the double-stranded RNA (dsRNA)-binding proteins Loquacious and r2d2, had normal expression levels. We identified an Aedes-specific paralogue of loquacious and r2d2, hereafter named loqs2, which is not expressed in the midgut. Loqs2 interacts with Loquacious and r2d2 and is required to control systemic replication of DENV and also Zika virus. Furthermore, ectopic expression of Loqs2 in the midgut of transgenic mosquitoes is sufficient to restrict DENV replication and dissemination. Together, our data reveal a mechanism of tissue-specific regulation of the mosquito siRNA pathway controlled by Loqs2. },

keywords = {Aedes aegypti, Dengue, imler, M3i, marois, Marques, Zika},

pubstate = {published},

tppubtype = {article}

}