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I2CT
Immunologie, Immunopathologie et Chimie Thérapeutique
Publications
2001
Rihn B., Saliou C., Bottin M. C., Keith G., Packer L.
From ancient remedies to modern therapeutics: pine bark uses in skin disorders revisited Article de journal
Dans: Phytother Res, vol. 15, no. 1, p. 76-8, 2001, (0951-418x Journal Article).
Résumé | BibTeX | Étiquettes: *Plants, Cell, Diseases/drug, effects, Expression, Flavonoids/*pharmacology/therapeutic, Gene, Human, Keratinocytes/*drug, Line/drug, Medicinal, Regulation, Skin, therapy/*genetics, use
@article{,
title = {From ancient remedies to modern therapeutics: pine bark uses in skin disorders revisited},
author = { B. Rihn and C. Saliou and M. C. Bottin and G. Keith and L. Packer},
year = {2001},
date = {2001-01-01},
journal = {Phytother Res},
volume = {15},
number = {1},
pages = {76-8},
abstract = {The effect of French maritime pine bark extract (PBE) on the gene expression profile of HaCaT human keratinocytes was studied using high density filter arrays. The expression profile of both control and PBE-treated cells was determined. Interestingly, PBE was shown to downregulate both calgranulin A and B genes which are known to be upregulated in psoriasis and various dermatoses. Thus, PBE could be considered in human dermatoses.},
note = {0951-418x
Journal Article},
keywords = {*Plants, Cell, Diseases/drug, effects, Expression, Flavonoids/*pharmacology/therapeutic, Gene, Human, Keratinocytes/*drug, Line/drug, Medicinal, Regulation, Skin, therapy/*genetics, use},
pubstate = {published},
tppubtype = {article}
}
The effect of French maritime pine bark extract (PBE) on the gene expression profile of HaCaT human keratinocytes was studied using high density filter arrays. The expression profile of both control and PBE-treated cells was determined. Interestingly, PBE was shown to downregulate both calgranulin A and B genes which are known to be upregulated in psoriasis and various dermatoses. Thus, PBE could be considered in human dermatoses.
1993
Pfeiffer P., Jung J. L., Heitzler J., Keith G.
Unusual structure of the double-stranded RNA associated with the '447' cytoplasmic male sterility in Vicia faba Article de journal
Dans: J Gen Virol, vol. 74, no. Pt 6, p. 1167-73, 1993, (0022-1317 Journal Article).
Résumé | BibTeX | Étiquettes: *Plants, &, Base, Bodies, Cytoplasm, Data, Extrachromosomal, Fabaceae/*genetics, Genetic, Inclusion, Infertility/genetics, Inheritance/*genetics, Medicinal, Molecular, Pollen/genetics, purification, Replicase/metabolism, RNA, RNA/*genetics/isolation, Sequence, Transcription, Viral
@article{,
title = {Unusual structure of the double-stranded RNA associated with the '447' cytoplasmic male sterility in Vicia faba},
author = { P. Pfeiffer and J. L. Jung and J. Heitzler and G. Keith},
year = {1993},
date = {1993-01-01},
journal = {J Gen Virol},
volume = {74},
number = {Pt 6},
pages = {1167-73},
abstract = {The 16.7 kbp dsRNA specific to the '447' cytoplasmic male sterility (CMS) line of Vicia faba was labelled in vitro with [alpha-32P]ATP and poly(A) polymerase, and by T4 RNA ligase-mediated addition of [32P]pCp. Analysis of the reaction products under denaturing conditions revealed in both cases extensive labelling of a 4.5 kb ssRNA, already detected in previous experiments in which the RNA-dependent RNA polymerase associated with the dsRNA was allowed to pursue RNA synthesis on preinitiated complexes. Mobility shift analysis of total pCp-labelled dsRNA revealed not two but three different 3' termini. The most prominent sequencing pattern corresponded to the 4.5 kb ssRNA, indicating that this RNA species has a preferentially accessible, free 3' OH extremity. Northern blot analysis of the denatured dsRNA confirmed that the 4.5 kb ssRNA is a subgenomic mRNA and detected its counterpart of about 12 kb. Nearly all 16.7 kbp dsRNA molecules featured an interrupted positive-sense strand, indicating a marked prevalence of transcription over replication complexes. This unusual strategy of transcription by a strand displacement mechanism, following initiation at an internal discontinuity, is compared with that of other dsRNA viruses or defective viruses, and is discussed in relation to the expression of the CMS trait.},
note = {0022-1317
Journal Article},
keywords = {*Plants, &, Base, Bodies, Cytoplasm, Data, Extrachromosomal, Fabaceae/*genetics, Genetic, Inclusion, Infertility/genetics, Inheritance/*genetics, Medicinal, Molecular, Pollen/genetics, purification, Replicase/metabolism, RNA, RNA/*genetics/isolation, Sequence, Transcription, Viral},
pubstate = {published},
tppubtype = {article}
}
The 16.7 kbp dsRNA specific to the '447' cytoplasmic male sterility (CMS) line of Vicia faba was labelled in vitro with [alpha-32P]ATP and poly(A) polymerase, and by T4 RNA ligase-mediated addition of [32P]pCp. Analysis of the reaction products under denaturing conditions revealed in both cases extensive labelling of a 4.5 kb ssRNA, already detected in previous experiments in which the RNA-dependent RNA polymerase associated with the dsRNA was allowed to pursue RNA synthesis on preinitiated complexes. Mobility shift analysis of total pCp-labelled dsRNA revealed not two but three different 3' termini. The most prominent sequencing pattern corresponded to the 4.5 kb ssRNA, indicating that this RNA species has a preferentially accessible, free 3' OH extremity. Northern blot analysis of the denatured dsRNA confirmed that the 4.5 kb ssRNA is a subgenomic mRNA and detected its counterpart of about 12 kb. Nearly all 16.7 kbp dsRNA molecules featured an interrupted positive-sense strand, indicating a marked prevalence of transcription over replication complexes. This unusual strategy of transcription by a strand displacement mechanism, following initiation at an internal discontinuity, is compared with that of other dsRNA viruses or defective viruses, and is discussed in relation to the expression of the CMS trait.