Postawski K., Olech-Fudali E., Korobowicz E., Jakowicki J. A., Keith G., Baranowski W.
[Hydrophobic DNA adducts in relationship to estrogen and progesterone receptors content in human uterine cancer.] Article de journal
Dans: Ginekol Pol, vol. 72, no. 9, p. 709-16, 2001, (0017-0011 Journal Article).
Résumé | BibTeX | Étiquettes: Abstract, Adducts/*analysis, Autoradiography, DNA, English, Estrogen/*analysis, Female, Human, Neoplasm/*analysis, Neoplasms/genetics/*pathology, Progesterone/*analysis, Receptors, Uterine
@article{,
title = {[Hydrophobic DNA adducts in relationship to estrogen and progesterone receptors content in human uterine cancer.]},
author = { K. Postawski and E. Olech-Fudali and E. Korobowicz and J. A. Jakowicki and G. Keith and W. Baranowski},
year = {2001},
date = {2001-01-01},
journal = {Ginekol Pol},
volume = {72},
number = {9},
pages = {709-16},
abstract = {OBJECTIVE: Determination of the relationship between hydrophobic DNA adducts (A) and estrogen receptors (ER) and progesterone (PR) receptor status in uterine cancers. METHODS: Using the P1 enriched version of 32P-postlabeling for hydrophobic DNA adducts detection on polyethyleneimine (PEI) cellulose thin layer chromatograms (TLC) we examined 11 uterine cancer DNAs. The quantification of the adducts was performed by Cerenkov counting of the spots. ER and PR status was recognized histochemically and H-score estimate was performed for each investigated cancer tissue. Patterns of uterine cancer DNA adducts were compared to the maps of adducts recognized in normal human endometrium. RESULTS: In three of the studied uterine cancers there was no positive staining of ER and PR; in one case there was a weak ER staining but PR staining was negative. In ER negative tumors the A level was significantly higher than in ER positive cancers (138.1 +/- 64.1 vs. 49.7 +/- 26.8 adducts per 10(9) nucleotides, respectively, p < 0.05). Highest A levels were found in two ER and PR negative G3 metastatic tumors. Finally, in all investigated cancers there was a strong, inverse correlation between ER content and A level (r = -0.67, p < 0.03). In addition, the correlation between PR level and A was of borderline significance (r = -0.6},
note = {0017-0011
Journal Article},
keywords = {Abstract, Adducts/*analysis, Autoradiography, DNA, English, Estrogen/*analysis, Female, Human, Neoplasm/*analysis, Neoplasms/genetics/*pathology, Progesterone/*analysis, Receptors, Uterine},
pubstate = {published},
tppubtype = {article}
}
Heitzler J., Marechal-Drouard L., Dirheimer G., Keith G.
Use of a dot blot hybridization method for identification of pure tRNA species on different membranes Article de journal
Dans: Biochim Biophys Acta-Gene Regul Mech, vol. 1129, no. 3, p. 273-7, 1992, (0006-3002 Journal Article).
Résumé | BibTeX | Étiquettes: *Membranes, Acid, Artificial, Autoradiography, cerevisiae/genetics, Fungal/genetics, Gov't, Hybridization, Met/genetics, Non-U.S., Nucleic, RNA, Saccharomyces, Support, Transfer, Transfer/*genetics
@article{,
title = {Use of a dot blot hybridization method for identification of pure tRNA species on different membranes},
author = { J. Heitzler and L. Marechal-Drouard and G. Dirheimer and G. Keith},
year = {1992},
date = {1992-01-01},
journal = {Biochim Biophys Acta-Gene Regul Mech},
volume = {1129},
number = {3},
pages = {273-7},
abstract = {The characterization of a tRNA in purification procedures usually involves aminoacylation assays but recently, the hybridization by dot blot with specific oligonucleotides as probes has been used for the tRNA identification. We present here an optimization of a dot blot hybridization method for the tRNA detection by comparing the efficiency of eight different nylon membranes. Neutral 0.22 microns porosity membranes (Nytran, Biodine A) give the best detection efficiency when small quantities of material (less than 40 ng of tRNA) are dotted on filter; by contrast, neutral 0.45 microns porosity membranes (such as Hybond N) are the most efficient when larger quantities of tRNA are dotted on the filter. The described technique allows to detect less than 20 pg of a pure tRNA species. Its use in the identification of Saccharomyces cerevisiae initiator tRNA(Met) in counter-current distribution fractions is shown.},
note = {0006-3002
Journal Article},
keywords = {*Membranes, Acid, Artificial, Autoradiography, cerevisiae/genetics, Fungal/genetics, Gov't, Hybridization, Met/genetics, Non-U.S., Nucleic, RNA, Saccharomyces, Support, Transfer, Transfer/*genetics},
pubstate = {published},
tppubtype = {article}
}