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2015
Marchesan Silvia, Kostarelos Kostas, Bianco Alberto, Prato Maurizio
The winding road for carbon nanotubes in nanomedicine Article de journal
Dans: Materials Today, vol. 18, no. 1, p. 12–19, 2015, ISSN: 1369-7021.
Résumé | Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{marchesan_winding_2015,
title = {The winding road for carbon nanotubes in nanomedicine},
author = {Silvia Marchesan and Kostas Kostarelos and Alberto Bianco and Maurizio Prato},
url = {http://www.sciencedirect.com/science/article/pii/S1369702114002594},
doi = {10.1016/j.mattod.2014.07.009},
issn = {1369-7021},
year = {2015},
date = {2015-01-01},
urldate = {2020-04-01},
journal = {Materials Today},
volume = {18},
number = {1},
pages = {12--19},
abstract = {Carbon nanotubes (CNTs) are recognized as promising nanomaterials for technological advancement. However, the stigma of structural similarity with asbestos fibers has slowed down progress of CNTs in nanomedicine. Nevertheless, it also prompted thorough studies that have revealed that functionalized CNTs (fCNTs) can biologically behave in a very different and safer manner. Here we review pristine and fCNT fate in biological settings, focusing on the importance of protein interaction, formation of the protein corona, and modulation of immune response. The emerging consensus on the desirable fCNT properties to achieve immunological neutrality, and even biodegradation, shows great promise for CNT adoption in medicine.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes (CNTs) are recognized as promising nanomaterials for technological advancement. However, the stigma of structural similarity with asbestos fibers has slowed down progress of CNTs in nanomedicine. Nevertheless, it also prompted thorough studies that have revealed that functionalized CNTs (fCNTs) can biologically behave in a very different and safer manner. Here we review pristine and fCNT fate in biological settings, focusing on the importance of protein interaction, formation of the protein corona, and modulation of immune response. The emerging consensus on the desirable fCNT properties to achieve immunological neutrality, and even biodegradation, shows great promise for CNT adoption in medicine.
2014
Marangon Iris, Ménard‐Moyon Cécilia, Kolosnjaj‐Tabi Jelena, Béoutis Marie Lys, Lartigue Lénaic, Alloyeau Damien, Pach Elzbieta, Ballesteros Belén, Autret Gwennhael, Ninjbadgar Tsedev, Brougham Dermot F, Bianco Alberto, Gazeau Florence
Covalent Functionalization of Multi-walled Carbon Nanotubes with a Gadolinium Chelate for Efficient T1-Weighted Magnetic Resonance Imaging Article de journal
Dans: Advanced Functional Materials, vol. 24, no. 45, p. 7173–7186, 2014, ISSN: 1616-3028.
Résumé | Liens | BibTeX | Étiquettes: Carbon nanotubes, contrast agents, I2CT, magnetic resonance imaging, Nanomedicine, Team-Bianco
@article{marangon_covalent_2014,
title = {Covalent Functionalization of Multi-walled Carbon Nanotubes with a Gadolinium Chelate for Efficient T1-Weighted Magnetic Resonance Imaging},
author = {Iris Marangon and Cécilia Ménard‐Moyon and Jelena Kolosnjaj‐Tabi and Marie Lys Béoutis and Lénaic Lartigue and Damien Alloyeau and Elzbieta Pach and Belén Ballesteros and Gwennhael Autret and Tsedev Ninjbadgar and Dermot F Brougham and Alberto Bianco and Florence Gazeau},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.201402234},
doi = {10.1002/adfm.201402234},
issn = {1616-3028},
year = {2014},
date = {2014-01-01},
urldate = {2020-04-02},
journal = {Advanced Functional Materials},
volume = {24},
number = {45},
pages = {7173--7186},
abstract = {Given the promise of carbon nanotubes (CNTs) for photothermal therapy, drug delivery, tissue engineering, and gene therapy, there is a need for non-invasive imaging methods to monitor CNT distribution and fate in the body. In this study, non-ionizing whole-body high field magnetic resonance imaging (MRI) is used to follow the distribution of water-dispersible non-toxic functionalized CNTs administrated intravenously to mice. Oxidized CNTs are endowed with positive MRI contrast properties by covalent functionalization with the chelating ligand diethylenetriaminepentaacetic dianhydride (DTPA), followed by chelation to Gd3+. The structural and magnetic properties, MR relaxivities, cellular uptake, and application for MRI cell imaging of Gd-CNTs in comparison to the precursor oxidized CNTs are evaluated. Despite the intrinsic T2 contrast of oxidized CNTs internalized in macrophages, the anchoring of paramagnetic gadolinium onto the nanotube sidewall allows efficient T1 contrast and MR signal enhancement, which is preserved after CNT internalization by cells. Hence, due to their high dispersibility, Gd-CNTs have the potential to produce positive contrast in vivo following injection into the bloodstream. The uptake of Gd-CNTs in the liver and spleen is assessed using MRI, while rapid renal clearance of extracellular Gd-CNTs is observed, confirming the evidences of other studies using different imaging modalities.},
keywords = {Carbon nanotubes, contrast agents, I2CT, magnetic resonance imaging, Nanomedicine, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Given the promise of carbon nanotubes (CNTs) for photothermal therapy, drug delivery, tissue engineering, and gene therapy, there is a need for non-invasive imaging methods to monitor CNT distribution and fate in the body. In this study, non-ionizing whole-body high field magnetic resonance imaging (MRI) is used to follow the distribution of water-dispersible non-toxic functionalized CNTs administrated intravenously to mice. Oxidized CNTs are endowed with positive MRI contrast properties by covalent functionalization with the chelating ligand diethylenetriaminepentaacetic dianhydride (DTPA), followed by chelation to Gd3+. The structural and magnetic properties, MR relaxivities, cellular uptake, and application for MRI cell imaging of Gd-CNTs in comparison to the precursor oxidized CNTs are evaluated. Despite the intrinsic T2 contrast of oxidized CNTs internalized in macrophages, the anchoring of paramagnetic gadolinium onto the nanotube sidewall allows efficient T1 contrast and MR signal enhancement, which is preserved after CNT internalization by cells. Hence, due to their high dispersibility, Gd-CNTs have the potential to produce positive contrast in vivo following injection into the bloodstream. The uptake of Gd-CNTs in the liver and spleen is assessed using MRI, while rapid renal clearance of extracellular Gd-CNTs is observed, confirming the evidences of other studies using different imaging modalities.
Lamanna Giuseppe, Grillaud Maxime, Macri Christophe, Chaloin Olivier, Muller Sylviane, Bianco Alberto
Adamantane-based dendrons for trimerization of the therapeutic P140 peptide Article de journal
Dans: Biomaterials, vol. 35, no. 26, p. 7553–7561, 2014, ISSN: 1878-5905.
Résumé | Liens | BibTeX | Étiquettes: Adamantane, Animals, Biotin, C3-symmetry, Dendrimers, Dendrons, Drug Carriers, Female, HSC70 Heat-Shock Proteins, HSPA8 protein, Humans, I2CT, Inbred MRL lpr, Lupus Erythematosus, Mice, P140 peptide, Peptide Fragments, Systemic, Team-Bianco
@article{lamanna_adamantane-based_2014,
title = {Adamantane-based dendrons for trimerization of the therapeutic P140 peptide},
author = {Giuseppe Lamanna and Maxime Grillaud and Christophe Macri and Olivier Chaloin and Sylviane Muller and Alberto Bianco},
doi = {10.1016/j.biomaterials.2014.05.017},
issn = {1878-5905},
year = {2014},
date = {2014-01-01},
journal = {Biomaterials},
volume = {35},
number = {26},
pages = {7553--7561},
abstract = {Dendrons constituted of an adamantane core, a focal point and three arms, were synthetized starting from a multifunctional adamantane derivative. Maleimido groups at the periphery of the scaffold were used to covalently attach the peptide called P140, a therapeutic phosphopeptide controlling disease activity in systemic lupus, both in mice and patients. Biotinylation of the trimers at the focal point was performed using click chemistry and the conjugates were studied in terms of solubility, binding affinity to its receptor, the HSPA8/HSC70 chaperone protein, effect on HSPA8 folding property and in vivo activity. The results showed that the trimerization of P140 peptide does not trigger aggregation or steric hindrances during the interaction with HSPA8 protein. Compared to the monomeric cognate peptide, the trivalent P140 peptide displayed the same capacity, in vitro, to down-regulate HSPA8 activity and, in vivo in MRL/lpr lupus-prone mice, to reduce abnormal blood hypercellularity. The control trimer synthesized with the same scaffold and a scrambled sequence of P140 showed no effect in vivo. This work reveals that adamantane-based scaffolds with a well-defined spatial conformation are promising trivalent systems for molecular recognition and for biomedical applications.},
keywords = {Adamantane, Animals, Biotin, C3-symmetry, Dendrimers, Dendrons, Drug Carriers, Female, HSC70 Heat-Shock Proteins, HSPA8 protein, Humans, I2CT, Inbred MRL lpr, Lupus Erythematosus, Mice, P140 peptide, Peptide Fragments, Systemic, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Dendrons constituted of an adamantane core, a focal point and three arms, were synthetized starting from a multifunctional adamantane derivative. Maleimido groups at the periphery of the scaffold were used to covalently attach the peptide called P140, a therapeutic phosphopeptide controlling disease activity in systemic lupus, both in mice and patients. Biotinylation of the trimers at the focal point was performed using click chemistry and the conjugates were studied in terms of solubility, binding affinity to its receptor, the HSPA8/HSC70 chaperone protein, effect on HSPA8 folding property and in vivo activity. The results showed that the trimerization of P140 peptide does not trigger aggregation or steric hindrances during the interaction with HSPA8 protein. Compared to the monomeric cognate peptide, the trivalent P140 peptide displayed the same capacity, in vitro, to down-regulate HSPA8 activity and, in vivo in MRL/lpr lupus-prone mice, to reduce abnormal blood hypercellularity. The control trimer synthesized with the same scaffold and a scrambled sequence of P140 showed no effect in vivo. This work reveals that adamantane-based scaffolds with a well-defined spatial conformation are promising trivalent systems for molecular recognition and for biomedical applications.
Servant A, Bianco A, Prato M, Kostarelos K
Graphene for multi-functional synthetic biology: the last 'zeitgeist' in nanomedicine Article de journal
Dans: Bioorganic & Medicinal Chemistry Letters, vol. 24, no. 7, p. 1638–1649, 2014, ISSN: 1464-3405.
Résumé | Liens | BibTeX | Étiquettes: Antineoplastic Agents, carbon, Drug delivery, Drug Design, Graphite, I2CT, Nanomaterials, Nanomedicine, nanotechnology, Synthetic Biology, Team-Bianco
@article{servant_graphene_2014,
title = {Graphene for multi-functional synthetic biology: the last 'zeitgeist' in nanomedicine},
author = {A Servant and A Bianco and M Prato and K Kostarelos},
doi = {10.1016/j.bmcl.2014.01.051},
issn = {1464-3405},
year = {2014},
date = {2014-01-01},
journal = {Bioorganic & Medicinal Chemistry Letters},
volume = {24},
number = {7},
pages = {1638--1649},
abstract = {The high versatility of graphene has attracted significant attention in many areas of scientific research from electronics to physics and mechanics. One of the most intriguing utilisation of graphene remains however in nanomedicine and synthetic biology. In particular, the last decade has witnessed an exponential growth in the generation of novel candidate therapeutics of multiple biological activities based on graphene constructs with small molecules, such as anti-cancer drugs. In this Digest, we summarise the different synthetic strategies and routes available to fabricate these promising graphene conjugates and the opportunities for the design of multi-functional tools for synthetic biology that they offer.},
keywords = {Antineoplastic Agents, carbon, Drug delivery, Drug Design, Graphite, I2CT, Nanomaterials, Nanomedicine, nanotechnology, Synthetic Biology, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
The high versatility of graphene has attracted significant attention in many areas of scientific research from electronics to physics and mechanics. One of the most intriguing utilisation of graphene remains however in nanomedicine and synthetic biology. In particular, the last decade has witnessed an exponential growth in the generation of novel candidate therapeutics of multiple biological activities based on graphene constructs with small molecules, such as anti-cancer drugs. In this Digest, we summarise the different synthetic strategies and routes available to fabricate these promising graphene conjugates and the opportunities for the design of multi-functional tools for synthetic biology that they offer.
2013
Bianco Alberto, Cheng Hui-Ming, Enoki Toshiaki, Gogotsi Yury, Hurt Robert H, Koratkar Nikhil, Kyotani Takashi, Monthioux Marc, Park Chong Rae, Tascon Juan M D, Zhang Jin
All in the graphene family – A recommended nomenclature for two-dimensional carbon materials Article de journal
Dans: Carbon, vol. 65, p. 1–6, 2013, ISSN: 0008-6223.
Résumé | Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{bianco_all_2013,
title = {All in the graphene family – A recommended nomenclature for two-dimensional carbon materials},
author = {Alberto Bianco and Hui-Ming Cheng and Toshiaki Enoki and Yury Gogotsi and Robert H Hurt and Nikhil Koratkar and Takashi Kyotani and Marc Monthioux and Chong Rae Park and Juan M D Tascon and Jin Zhang},
url = {http://www.sciencedirect.com/science/article/pii/S0008622313008002},
doi = {10.1016/j.carbon.2013.08.038},
issn = {0008-6223},
year = {2013},
date = {2013-12-01},
urldate = {2020-03-31},
journal = {Carbon},
volume = {65},
pages = {1--6},
abstract = {Interest in two-dimensional, sheet-like or flake-like carbon forms has expanded beyond monolayer graphene to include related materials with significant variations in layer number, lateral dimension, rotational faulting, and chemical modification. Describing this family of “graphene materials” has been causing confusion in the Carbon journal and in the scientific literature as a whole. The international editorial team for Carbon believes that the time has come for a discussion on a rational naming system for two-dimensional carbon forms. We propose here a first nomenclature for two-dimensional carbons that could guide authors toward a more precise description of their subject materials, and could allow the field to move forward with a higher degree of common understanding.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Interest in two-dimensional, sheet-like or flake-like carbon forms has expanded beyond monolayer graphene to include related materials with significant variations in layer number, lateral dimension, rotational faulting, and chemical modification. Describing this family of “graphene materials” has been causing confusion in the Carbon journal and in the scientific literature as a whole. The international editorial team for Carbon believes that the time has come for a discussion on a rational naming system for two-dimensional carbon forms. We propose here a first nomenclature for two-dimensional carbons that could guide authors toward a more precise description of their subject materials, and could allow the field to move forward with a higher degree of common understanding.
Serag Maged F, Kaji Noritada, Habuchi Satoshi, Bianco Alberto, Baba Yoshinobu
Nanobiotechnology meets plant cell biology: carbon nanotubes as organelle targeting nanocarriers Article de journal
Dans: RSC Advances, vol. 3, no. 15, p. 4856–4862, 2013, ISSN: 2046-2069.
Résumé | Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{serag_nanobiotechnology_2013,
title = {Nanobiotechnology meets plant cell biology: carbon nanotubes as organelle targeting nanocarriers},
author = {Maged F Serag and Noritada Kaji and Satoshi Habuchi and Alberto Bianco and Yoshinobu Baba},
url = {https://pubs.rsc.org/en/content/articlelanding/2013/ra/c2ra22766e},
doi = {10.1039/C2RA22766E},
issn = {2046-2069},
year = {2013},
date = {2013-03-01},
urldate = {2020-04-01},
journal = {RSC Advances},
volume = {3},
number = {15},
pages = {4856--4862},
abstract = {For years, nanotechnology has shown great promise in the fields of biomedical and biotechnological sciences and medical research. In this review, we demonstrate its versatility and applicability in plant cell biology studies. Specifically, we discuss the ability of functionalized carbon nanotubes to penetrate the plant cell wall, target specific organelles, probe protein-carrier activity and induce organelle recycling in plant cells. We also, shed light on prospective applications of carbon nanomaterials in cell biology and plant cell transformation.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
For years, nanotechnology has shown great promise in the fields of biomedical and biotechnological sciences and medical research. In this review, we demonstrate its versatility and applicability in plant cell biology studies. Specifically, we discuss the ability of functionalized carbon nanotubes to penetrate the plant cell wall, target specific organelles, probe protein-carrier activity and induce organelle recycling in plant cells. We also, shed light on prospective applications of carbon nanomaterials in cell biology and plant cell transformation.
Boscolo S, Pelin M, Bortoli M De, Fontanive G, Barreras A, Berti F, Sosa S, Chaloin O, Bianco A, Yasumoto T, Prato M, Poli M, Tubaro A
Sandwich ELISA Assay for the Quantitation of Palytoxin and Its Analogs in Natural Samples Article de journal
Dans: Environmental Science & Technology, vol. 47, no. 4, p. 2034–2042, 2013, ISSN: 0013-936X.
Résumé | Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{boscolo_sandwich_2013,
title = {Sandwich ELISA Assay for the Quantitation of Palytoxin and Its Analogs in Natural Samples},
author = {S Boscolo and M Pelin and M De Bortoli and G Fontanive and A Barreras and F Berti and S Sosa and O Chaloin and A Bianco and T Yasumoto and M Prato and M Poli and A Tubaro},
url = {https://doi.org/10.1021/es304222t},
doi = {10.1021/es304222t},
issn = {0013-936X},
year = {2013},
date = {2013-02-01},
urldate = {2020-04-01},
journal = {Environmental Science & Technology},
volume = {47},
number = {4},
pages = {2034--2042},
abstract = {Palytoxins are potent marine biotoxins that have recently become endemic to the Mediterranean Sea, and are becoming more frequently associated with seafood. Due to their high toxicity, suitable methods to quantify palytoxins are needed. Thus, we developed an indirect sandwich ELISA for palytoxin and 42-hydroxy-palytoxin. An intralaboratory study demonstrated sensitivity (limit of detection},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Palytoxins are potent marine biotoxins that have recently become endemic to the Mediterranean Sea, and are becoming more frequently associated with seafood. Due to their high toxicity, suitable methods to quantify palytoxins are needed. Thus, we developed an indirect sandwich ELISA for palytoxin and 42-hydroxy-palytoxin. An intralaboratory study demonstrated sensitivity (limit of detection
Russier Julie, Treossi Emanuele, Scarsi Alessia, Perrozzi Francesco, Dumortier Hélène, Ottaviano Luca, Meneghetti Moreno, Palermo Vincenzo, Bianco Alberto
Evidencing the mask effect of graphene oxide: a comparative study on primary human and murine phagocytic cells Article de journal
Dans: Nanoscale, vol. 5, no. 22, p. 11234–11247, 2013, ISSN: 2040-3372.
Résumé | Liens | BibTeX | Étiquettes: Animals, Cell Survival, Cells, Cultured, Cytokines, Dumortier, Graphite, Humans, I2CT, Macrophages, Mice, Monocytes, Oxidative Stress, Oxides, Reactive Oxygen Species, Team-Bianco, Team-Dumortier
@article{russier_evidencing_2013,
title = {Evidencing the mask effect of graphene oxide: a comparative study on primary human and murine phagocytic cells},
author = {Julie Russier and Emanuele Treossi and Alessia Scarsi and Francesco Perrozzi and Hélène Dumortier and Luca Ottaviano and Moreno Meneghetti and Vincenzo Palermo and Alberto Bianco},
doi = {10.1039/c3nr03543c},
issn = {2040-3372},
year = {2013},
date = {2013-01-01},
journal = {Nanoscale},
volume = {5},
number = {22},
pages = {11234--11247},
abstract = {Graphene oxide (GO) is attracting an ever-growing interest in different fields and applications. Not much is known about the possible impact of GO sheet lateral dimensions on their effects in vitro, especially on human primary cells. In an attempt to address this issue, we present a study to evaluate, how highly soluble 2-dimensional GO constituted of large or small flakes affects human monocyte derived macrophages (hMDM). For this purpose, the lateral size of GO was tuned using sonication and three samples were obtained. The non sonicated one presented large flakes (textasciitilde1.32 μm) while sonication for 2 and 26 hours generated small (textasciitilde0.27 μm) and very small (textasciitilde0.13 μm) sheets of GO, respectively. Cell studies were then conducted to evaluate the cytotoxicity, the oxidative stress induction, the activation potential and the pro-inflammatory effects of these different types of GO at increasing concentrations. In comparison, the same experiments were run on murine intraperitoneal macrophages (mIPM). The interaction between GO and cells was further examined by TEM and Raman spectroscopy. Our data revealed that the GO sheet size had a significant impact on different cellular parameters (i.e. cellular viability, ROS generation, and cellular activation). Indeed, the more the lateral dimensions of GO were reduced, the higher were the cellular internalization and the effects on cellular functionality. Our data also revealed a particular interaction of GO flakes with the cellular membrane. In fact, a GO mask due to the parallel arrangement of the graphene sheets on the cellular surface was observed. Considering the mask effect, we have hypothesized that this particular contact between GO sheets and the cell membrane could either promote their internalization or isolate cells from their environment, thus possibly accounting for the following impact on cellular parameters.},
keywords = {Animals, Cell Survival, Cells, Cultured, Cytokines, Dumortier, Graphite, Humans, I2CT, Macrophages, Mice, Monocytes, Oxidative Stress, Oxides, Reactive Oxygen Species, Team-Bianco, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}
Graphene oxide (GO) is attracting an ever-growing interest in different fields and applications. Not much is known about the possible impact of GO sheet lateral dimensions on their effects in vitro, especially on human primary cells. In an attempt to address this issue, we present a study to evaluate, how highly soluble 2-dimensional GO constituted of large or small flakes affects human monocyte derived macrophages (hMDM). For this purpose, the lateral size of GO was tuned using sonication and three samples were obtained. The non sonicated one presented large flakes (textasciitilde1.32 μm) while sonication for 2 and 26 hours generated small (textasciitilde0.27 μm) and very small (textasciitilde0.13 μm) sheets of GO, respectively. Cell studies were then conducted to evaluate the cytotoxicity, the oxidative stress induction, the activation potential and the pro-inflammatory effects of these different types of GO at increasing concentrations. In comparison, the same experiments were run on murine intraperitoneal macrophages (mIPM). The interaction between GO and cells was further examined by TEM and Raman spectroscopy. Our data revealed that the GO sheet size had a significant impact on different cellular parameters (i.e. cellular viability, ROS generation, and cellular activation). Indeed, the more the lateral dimensions of GO were reduced, the higher were the cellular internalization and the effects on cellular functionality. Our data also revealed a particular interaction of GO flakes with the cellular membrane. In fact, a GO mask due to the parallel arrangement of the graphene sheets on the cellular surface was observed. Considering the mask effect, we have hypothesized that this particular contact between GO sheets and the cell membrane could either promote their internalization or isolate cells from their environment, thus possibly accounting for the following impact on cellular parameters.
2012
Singh Prabhpreet, Ménard-Moyon Cécilia, Kumar Jitendra, Fabre Bruno, Verma Sandeep, Bianco Alberto
Nucleobase-pairing triggers the self-assembly of uracil-ferrocene on adenine functionalized multi-walled carbon nanotubes Article de journal
Dans: Carbon, vol. 50, no. 9, p. 3170–3177, 2012, ISSN: 0008-6223.
Résumé | Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{singh_nucleobase-pairing_2012,
title = {Nucleobase-pairing triggers the self-assembly of uracil-ferrocene on adenine functionalized multi-walled carbon nanotubes},
author = {Prabhpreet Singh and Cécilia Ménard-Moyon and Jitendra Kumar and Bruno Fabre and Sandeep Verma and Alberto Bianco},
url = {http://www.sciencedirect.com/science/article/pii/S0008622311008670},
doi = {10.1016/j.carbon.2011.10.037},
issn = {0008-6223},
year = {2012},
date = {2012-08-01},
urldate = {2020-03-31},
journal = {Carbon},
volume = {50},
number = {9},
pages = {3170--3177},
series = {Festschrift dedicated to Peter A. Thrower, Editor-in-Chief, 1972 - 2012},
abstract = {Shortened and oxidized multi-walled carbon nanotubes (MWCNTs) were functionalized with adenine using the amidation strategy. The adenine functionalized MWCNTs (Ad-MWCNTs) were complexed with a uracil substituted ferrocene and characterized by transmission electron microscopy (TEM), high resolution TEM (HRTEM), electron diffraction X-ray spectroscopy (EDX), and atomic force microscopy (AFM). The electrochemical properties of these novel nanohybrids were studied by cyclic voltammetry. The favorable supramolecular interaction of the electroactive species with the functionalized nanotubes through the efficient adenine–uracil base-pairing can be exploited for the design of new electronic devices.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Shortened and oxidized multi-walled carbon nanotubes (MWCNTs) were functionalized with adenine using the amidation strategy. The adenine functionalized MWCNTs (Ad-MWCNTs) were complexed with a uracil substituted ferrocene and characterized by transmission electron microscopy (TEM), high resolution TEM (HRTEM), electron diffraction X-ray spectroscopy (EDX), and atomic force microscopy (AFM). The electrochemical properties of these novel nanohybrids were studied by cyclic voltammetry. The favorable supramolecular interaction of the electroactive species with the functionalized nanotubes through the efficient adenine–uracil base-pairing can be exploited for the design of new electronic devices.
Serag Maged F, Kaji Noritada, Tokeshi Manabu, Bianco Alberto, Baba Yoshinobu
The plant cell uses carbon nanotubes to build tracheary elements Article de journal
Dans: Integrative Biology: Quantitative Biosciences from Nano to Macro, vol. 4, no. 2, p. 127–131, 2012, ISSN: 1757-9708.
Résumé | Liens | BibTeX | Étiquettes: Arabidopsis, Atomic Force, carbon, Cell Differentiation, Confocal, Endocytosis, I2CT, Lignin, Microscopy, Nanotubes, Plant Cells, Team-Bianco
@article{serag_plant_2012,
title = {The plant cell uses carbon nanotubes to build tracheary elements},
author = {Maged F Serag and Noritada Kaji and Manabu Tokeshi and Alberto Bianco and Yoshinobu Baba},
doi = {10.1039/c2ib00135g},
issn = {1757-9708},
year = {2012},
date = {2012-02-01},
journal = {Integrative Biology: Quantitative Biosciences from Nano to Macro},
volume = {4},
number = {2},
pages = {127--131},
abstract = {Since their discovery, carbon nanotubes (CNTs) have been eminent members of the nanomaterial family. Because of their unique physical, chemical and mechanical properties, they are regarded as new potential materials to bring enormous benefits in cell biology studies. Undoubtedly, the first step to prove the advantages of CNTs is to understand the basic behavior of CNTs inside the cells. In a number of studies, CNTs have been demonstrated as new carrier systems for the delivery of DNA, proteins and therapeutic molecules into living cells. However, post-uptake behavior of CNTs inside the cells has not received much consideration. Utilizing the plant cell model, we have shown in this study that the plant cells, differentiating into tracheary elements, incorporate cup-stacked carbon nanotubes (CSCNTs) into cell structure via oxidative cross-linking of monolignols to the nanotubes surface during lignin biosynthesis. This finding highlights the fate of CNTs inside plant cells and provides an example on how the plant cell can handle internalized carbon nanomaterials.},
keywords = {Arabidopsis, Atomic Force, carbon, Cell Differentiation, Confocal, Endocytosis, I2CT, Lignin, Microscopy, Nanotubes, Plant Cells, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Since their discovery, carbon nanotubes (CNTs) have been eminent members of the nanomaterial family. Because of their unique physical, chemical and mechanical properties, they are regarded as new potential materials to bring enormous benefits in cell biology studies. Undoubtedly, the first step to prove the advantages of CNTs is to understand the basic behavior of CNTs inside the cells. In a number of studies, CNTs have been demonstrated as new carrier systems for the delivery of DNA, proteins and therapeutic molecules into living cells. However, post-uptake behavior of CNTs inside the cells has not received much consideration. Utilizing the plant cell model, we have shown in this study that the plant cells, differentiating into tracheary elements, incorporate cup-stacked carbon nanotubes (CSCNTs) into cell structure via oxidative cross-linking of monolignols to the nanotubes surface during lignin biosynthesis. This finding highlights the fate of CNTs inside plant cells and provides an example on how the plant cell can handle internalized carbon nanomaterials.
Delogu Lucia Gemma, Venturelli Enrica, Manetti Roberto, Pinna Gérard Aimé, Carru Ciriaco, Madeddu Roberto, Murgia Luciano, Sgarrella Francesco, Dumortier Hélène, Bianco Alberto
Ex vivo impact of functionalized carbon nanotubes on human immune cells Article de journal
Dans: Nanomedicine (London, England), vol. 7, no. 2, p. 231–243, 2012, ISSN: 1748-6963.
Résumé | Liens | BibTeX | Étiquettes: carbon, Cells, Cultured, Cytokines, Dumortier, Humans, I2CT, Immunity, Innate, Materials Testing, Nanotubes, T-Lymphocytes, Team-Bianco, Team-Dumortier
@article{delogu_ex_2012,
title = {Ex vivo impact of functionalized carbon nanotubes on human immune cells},
author = {Lucia Gemma Delogu and Enrica Venturelli and Roberto Manetti and Gérard Aimé Pinna and Ciriaco Carru and Roberto Madeddu and Luciano Murgia and Francesco Sgarrella and Hélène Dumortier and Alberto Bianco},
doi = {10.2217/nnm.11.101},
issn = {1748-6963},
year = {2012},
date = {2012-02-01},
journal = {Nanomedicine (London, England)},
volume = {7},
number = {2},
pages = {231--243},
abstract = {AIM: Different studies, carried out by us and others, have investigated the impact of carbon nanotubes (CNTs) in vitro and in animal models. To date, only a few studies have been performed on human cells ex vivo. There is also a lack of comparison between CNTs with varied functionalization and structural properties and their impact on different cell types.
MATERIALS & METHODS: The present ex vivo human study focuses on the impact of a series of functionalized multiwalled CNTs on human T and B lymphocytes, natural killer (NK) cells and monocytes.
RESULTS: Smaller diameter nanotubes are internalized more efficiently. Viability assays displayed the absence of cytotoxicity of all multiwalled CNTs used. Activation assay demonstrated a strong effect on monocytes and NK cells.
CONCLUSION: Our results, on human cells ex vivo, confirmed previous studies demonstrating appropriately functionalized CNTs are nontoxic. The effects on cell functionality were significant for the monocytes and NK cells. These findings encourage the possible use of CNTs for biomedical applications either as carriers of therapeutic molecules or as immune modulator systems.},
keywords = {carbon, Cells, Cultured, Cytokines, Dumortier, Humans, I2CT, Immunity, Innate, Materials Testing, Nanotubes, T-Lymphocytes, Team-Bianco, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}
AIM: Different studies, carried out by us and others, have investigated the impact of carbon nanotubes (CNTs) in vitro and in animal models. To date, only a few studies have been performed on human cells ex vivo. There is also a lack of comparison between CNTs with varied functionalization and structural properties and their impact on different cell types.
MATERIALS & METHODS: The present ex vivo human study focuses on the impact of a series of functionalized multiwalled CNTs on human T and B lymphocytes, natural killer (NK) cells and monocytes.
RESULTS: Smaller diameter nanotubes are internalized more efficiently. Viability assays displayed the absence of cytotoxicity of all multiwalled CNTs used. Activation assay demonstrated a strong effect on monocytes and NK cells.
CONCLUSION: Our results, on human cells ex vivo, confirmed previous studies demonstrating appropriately functionalized CNTs are nontoxic. The effects on cell functionality were significant for the monocytes and NK cells. These findings encourage the possible use of CNTs for biomedical applications either as carriers of therapeutic molecules or as immune modulator systems.
MATERIALS & METHODS: The present ex vivo human study focuses on the impact of a series of functionalized multiwalled CNTs on human T and B lymphocytes, natural killer (NK) cells and monocytes.
RESULTS: Smaller diameter nanotubes are internalized more efficiently. Viability assays displayed the absence of cytotoxicity of all multiwalled CNTs used. Activation assay demonstrated a strong effect on monocytes and NK cells.
CONCLUSION: Our results, on human cells ex vivo, confirmed previous studies demonstrating appropriately functionalized CNTs are nontoxic. The effects on cell functionality were significant for the monocytes and NK cells. These findings encourage the possible use of CNTs for biomedical applications either as carriers of therapeutic molecules or as immune modulator systems.
Lamanna Giuseppe, Russier Julie, Dumortier Hélène, Bianco Alberto
Enhancement of anti-inflammatory drug activity by multivalent adamantane-based dendrons Article de journal
Dans: Biomaterials, vol. 33, no. 22, p. 5610–5617, 2012, ISSN: 1878-5905.
Résumé | Liens | BibTeX | Étiquettes: Animals, Anti-Inflammatory Agents, Cell Line, Cell Survival, Dendrimers, Drug Synergism, Dumortier, I2CT, Ibuprofen, Macrophages, Mice, Team-Bianco, Team-Dumortier
@article{lamanna_enhancement_2012,
title = {Enhancement of anti-inflammatory drug activity by multivalent adamantane-based dendrons},
author = {Giuseppe Lamanna and Julie Russier and Hélène Dumortier and Alberto Bianco},
doi = {10.1016/j.biomaterials.2012.03.072},
issn = {1878-5905},
year = {2012},
date = {2012-01-01},
journal = {Biomaterials},
volume = {33},
number = {22},
pages = {5610--5617},
abstract = {We have developed a straightforward method to prepare 1(st) and 2(nd) generation adamantane-based dendrons, previously called HYDRAmers, bearing at the periphery the anti-inflammatory drug, ibuprofen. The multivalency effect on the drug activity was studied, demonstrating that our multivalent ibuprofen-dendron conjugates exert an enhanced anti-inflammatory activity compared to free ibuprofen, in vitro. These results provide insights into the effect of HYDRAmer multivalency on biological interactions for therapeutic applications.},
keywords = {Animals, Anti-Inflammatory Agents, Cell Line, Cell Survival, Dendrimers, Drug Synergism, Dumortier, I2CT, Ibuprofen, Macrophages, Mice, Team-Bianco, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}
We have developed a straightforward method to prepare 1(st) and 2(nd) generation adamantane-based dendrons, previously called HYDRAmers, bearing at the periphery the anti-inflammatory drug, ibuprofen. The multivalency effect on the drug activity was studied, demonstrating that our multivalent ibuprofen-dendron conjugates exert an enhanced anti-inflammatory activity compared to free ibuprofen, in vitro. These results provide insights into the effect of HYDRAmer multivalency on biological interactions for therapeutic applications.
Lacerda Lara, Russier Julie, Pastorin Giorgia, Herrero Antonia M, Venturelli Enrica, Dumortier Hélène, Al-Jamal Khuloud T, Prato Maurizio, Kostarelos Kostas, Bianco Alberto
Translocation mechanisms of chemically functionalised carbon nanotubes across plasma membranes Article de journal
Dans: Biomaterials, vol. 33, no. 11, p. 3334–3343, 2012, ISSN: 1878-5905.
Résumé | Liens | BibTeX | Étiquettes: Animals, carbon, Cell Line, Cell Membrane, Dumortier, I2CT, Macrophages, Mice, Nanotubes, Phagocytosis, Team-Bianco, Team-Dumortier
@article{lacerda_translocation_2012,
title = {Translocation mechanisms of chemically functionalised carbon nanotubes across plasma membranes},
author = {Lara Lacerda and Julie Russier and Giorgia Pastorin and Antonia M Herrero and Enrica Venturelli and Hélène Dumortier and Khuloud T Al-Jamal and Maurizio Prato and Kostas Kostarelos and Alberto Bianco},
doi = {10.1016/j.biomaterials.2012.01.024},
issn = {1878-5905},
year = {2012},
date = {2012-01-01},
journal = {Biomaterials},
volume = {33},
number = {11},
pages = {3334--3343},
abstract = {Understanding the mechanisms responsible for carbon nanotube (CNT) internalisation into live cells is considered critical both from a fundamental point of view and for further engineering of CNT-based delivery systems to intracellular targets. While several studies are focused on the development of such CNT-based delivery systems, attempts to systematically elucidate the cellular uptake mechanisms of CNTs are still rather limited. The aim of the present study is to evaluate the cellular internalisation of chemically functionalised multi-walled carbon nanotubes (f-MWCNTs) in the presence of different well-known cellular uptake inhibitors. Our data reveal how f-MWCNTs are able to translocate across cell membranes of both phagocytic and non-phagocytic cell lines. We have evidenced that at least 30-50% of f-MWCNTs are taken up by cells through an energy-independent mechanism. This characteristic makes nanotubes loaded with therapeutic or diagnostic cargos extremely interesting as the release of active molecules directly into the cytoplasm increase their biological activity and therapeutic efficacy.},
keywords = {Animals, carbon, Cell Line, Cell Membrane, Dumortier, I2CT, Macrophages, Mice, Nanotubes, Phagocytosis, Team-Bianco, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}
Understanding the mechanisms responsible for carbon nanotube (CNT) internalisation into live cells is considered critical both from a fundamental point of view and for further engineering of CNT-based delivery systems to intracellular targets. While several studies are focused on the development of such CNT-based delivery systems, attempts to systematically elucidate the cellular uptake mechanisms of CNTs are still rather limited. The aim of the present study is to evaluate the cellular internalisation of chemically functionalised multi-walled carbon nanotubes (f-MWCNTs) in the presence of different well-known cellular uptake inhibitors. Our data reveal how f-MWCNTs are able to translocate across cell membranes of both phagocytic and non-phagocytic cell lines. We have evidenced that at least 30-50% of f-MWCNTs are taken up by cells through an energy-independent mechanism. This characteristic makes nanotubes loaded with therapeutic or diagnostic cargos extremely interesting as the release of active molecules directly into the cytoplasm increase their biological activity and therapeutic efficacy.
Lamanna Giuseppe, Battigelli Alessia, Ménard-Moyon Cécilia, Bianco Alberto
Multifunctionalized carbon nanotubes as advanced multimodal nanomaterials for biomedical applications Article de journal
Dans: Nanotechnology Reviews, vol. 1, no. 1, p. 17–29, 2012, ISSN: 2191-9089, 2191-9097.
Résumé | Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{lamanna_multifunctionalized_2012,
title = {Multifunctionalized carbon nanotubes as advanced multimodal nanomaterials for biomedical applications},
author = {Giuseppe Lamanna and Alessia Battigelli and Cécilia Ménard-Moyon and Alberto Bianco},
url = {https://www.degruyter.com/view/journals/ntrev/1/1/article-p17.xml},
doi = {10.1515/ntrev-2011-0002},
issn = {2191-9089, 2191-9097},
year = {2012},
date = {2012-01-01},
urldate = {2020-04-01},
journal = {Nanotechnology Reviews},
volume = {1},
number = {1},
pages = {17--29},
abstract = {textbackslashtextlesssection class="abstract"textbackslashtextgreatertextbackslashtextlessh2 class="abstractTitle text-title my-1" id="d739e2"textbackslashtextgreaterAbstracttextbackslashtextless/h2textbackslashtextgreatertextbackslashtextlessptextbackslashtextgreaterThe increasing importance of nanotechnology in the field of biomedical applications has encouraged the development of new nanomaterials endowed with multiple functions. Novel nanoscale drug delivery systems with diagnostic, imaging and therapeutic properties hold many promises for the treatment of different types of diseases, including cancer, infection and neurodegenerative syndromes. Functionalized carbon nanotubes (CNTs) are one of the most recent type of nanomaterial developed in biomedicine as they can be designed and imparted with multimodal capabilities. Indeed, the possibility of inserting different functionalities on CNTs is opening the possibility to exploit them on new strategies that combine diagnosis with improved therapeutic efficacies. In this review, we describe the different approaches that have been recently developed to generate multifunctionalized CNTs for biomedical applications. In particular, covalent and non-covalent double and triple functionalization methods are discussed, putting in evidence their use textbackslashtextlessemtextbackslashtextgreaterin vitrotextbackslashtextless/emtextbackslashtextgreater and textbackslashtextlessemtextbackslashtextgreaterin vivotextbackslashtextless/emtextbackslashtextgreater and highlighting the advantages and the drawbacks of these new systems. Preclinical studies have demonstrated that multifunctional CNTs are highly promising when combining diagnostic, imaging and therapeutic modalities.textbackslashtextless/ptextbackslashtextgreatertextbackslashtextless/sectiontextbackslashtextgreater},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
textbackslashtextlesssection class="abstract"textbackslashtextgreatertextbackslashtextlessh2 class="abstractTitle text-title my-1" id="d739e2"textbackslashtextgreaterAbstracttextbackslashtextless/h2textbackslashtextgreatertextbackslashtextlessptextbackslashtextgreaterThe increasing importance of nanotechnology in the field of biomedical applications has encouraged the development of new nanomaterials endowed with multiple functions. Novel nanoscale drug delivery systems with diagnostic, imaging and therapeutic properties hold many promises for the treatment of different types of diseases, including cancer, infection and neurodegenerative syndromes. Functionalized carbon nanotubes (CNTs) are one of the most recent type of nanomaterial developed in biomedicine as they can be designed and imparted with multimodal capabilities. Indeed, the possibility of inserting different functionalities on CNTs is opening the possibility to exploit them on new strategies that combine diagnosis with improved therapeutic efficacies. In this review, we describe the different approaches that have been recently developed to generate multifunctionalized CNTs for biomedical applications. In particular, covalent and non-covalent double and triple functionalization methods are discussed, putting in evidence their use textbackslashtextlessemtextbackslashtextgreaterin vitrotextbackslashtextless/emtextbackslashtextgreater and textbackslashtextlessemtextbackslashtextgreaterin vivotextbackslashtextless/emtextbackslashtextgreater and highlighting the advantages and the drawbacks of these new systems. Preclinical studies have demonstrated that multifunctional CNTs are highly promising when combining diagnostic, imaging and therapeutic modalities.textbackslashtextless/ptextbackslashtextgreatertextbackslashtextless/sectiontextbackslashtextgreater
Fabre Bruno, Samorì Cristian, Bianco Alberto
Immobilization of double functionalized carbon nanotubes on glassy carbon electrodes for the electrochemical sensing of the biotin–avidin affinity Article de journal
Dans: Journal of Electroanalytical Chemistry, vol. 665, p. 90–94, 2012, ISSN: 1572-6657.
Résumé | Liens | BibTeX | Étiquettes: Avidin, Biotin, Electrochemical detection, Ferrocene, Functionalized carbon nanotubes, I2CT, Nafion, Team-Bianco
@article{fabre_immobilization_2012,
title = {Immobilization of double functionalized carbon nanotubes on glassy carbon electrodes for the electrochemical sensing of the biotin–avidin affinity},
author = {Bruno Fabre and Cristian Samorì and Alberto Bianco},
url = {http://www.sciencedirect.com/science/article/pii/S1572665711005893},
doi = {10.1016/j.jelechem.2011.11.029},
issn = {1572-6657},
year = {2012},
date = {2012-01-01},
urldate = {2020-04-01},
journal = {Journal of Electroanalytical Chemistry},
volume = {665},
pages = {90--94},
abstract = {Multi-walled carbon nanotubes (MWCNTs) double functionalized with redox-active ferrocene and biotin (Fc–Biot-MWCNTs) were synthesized and used for the electrochemical detection of avidin. After dispersion in perfluorosulfonated polymer Nafion and immobilization on the electrode surfaces, the cyclic voltammetry response of the modified electrodes showed in aqueous medium a quasi-reversible one-electron system at 0.46V vs. SCE, assigned to the bound ferrocene/ferrocenium redox couple. Upon the addition of avidin in the range 0.9–20nM, a stepwise decrease of both anodic and cathodic peak currents ascribed to the ferrocene was observed. These electrochemical changes are specifically due to the formation of biotin–avidin complex and are explained by complexation-induced modifications in the environment of covalently bound ferrocene.},
keywords = {Avidin, Biotin, Electrochemical detection, Ferrocene, Functionalized carbon nanotubes, I2CT, Nafion, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Multi-walled carbon nanotubes (MWCNTs) double functionalized with redox-active ferrocene and biotin (Fc–Biot-MWCNTs) were synthesized and used for the electrochemical detection of avidin. After dispersion in perfluorosulfonated polymer Nafion and immobilization on the electrode surfaces, the cyclic voltammetry response of the modified electrodes showed in aqueous medium a quasi-reversible one-electron system at 0.46V vs. SCE, assigned to the bound ferrocene/ferrocenium redox couple. Upon the addition of avidin in the range 0.9–20nM, a stepwise decrease of both anodic and cathodic peak currents ascribed to the ferrocene was observed. These electrochemical changes are specifically due to the formation of biotin–avidin complex and are explained by complexation-induced modifications in the environment of covalently bound ferrocene.
2011
Serag Maged F, Kaji Noritada, Venturelli Enrica, Okamoto Yukihiro, Terasaka Kazuyoshi, Tokeshi Manabu, Mizukami Hajime, Braeckmans Kevin, Bianco Alberto, Baba Yoshinobu
Functional platform for controlled subcellular distribution of carbon nanotubes Article de journal
Dans: ACS nano, vol. 5, no. 11, p. 9264–9270, 2011, ISSN: 1936-086X.
Résumé | Liens | BibTeX | Étiquettes: Biological Transport, carbon, Catharanthus, Exocytosis, Fluorescence Recovery After Photobleaching, Fluorescent Dyes, I2CT, Intracellular Space, Nanotubes, Surface Properties, Team-Bianco, Vacuoles
@article{serag_functional_2011,
title = {Functional platform for controlled subcellular distribution of carbon nanotubes},
author = {Maged F Serag and Noritada Kaji and Enrica Venturelli and Yukihiro Okamoto and Kazuyoshi Terasaka and Manabu Tokeshi and Hajime Mizukami and Kevin Braeckmans and Alberto Bianco and Yoshinobu Baba},
doi = {10.1021/nn2035654},
issn = {1936-086X},
year = {2011},
date = {2011-11-01},
journal = {ACS nano},
volume = {5},
number = {11},
pages = {9264--9270},
abstract = {As nanoparticles can cross different cellular barriers and access different tissues, control of their uptake and cellular fate presents a functional approach that will be broadly applicable to nanoscale technologies in cell biology. Here we show that the trafficking of single-walled carbon nanotubes (SWCNTs) through various subcellular membranes of the plant cell is facilitated or inhibited by attaching a suitable functional tag and controlling medium components. This enables a unique control over the uptake and the subcellular distribution of SWCNTs and provides a key strategy to promote their cellular elimination to minimize toxicity. Our results also demonstrate that SWCNTs are involved in a carrier-mediated transport (CMT) inside cells; this is a phenomenon that scientists could use to obtain novel molecular insights into CMT, with the potential translation to advances in subcellular nanobiology.},
keywords = {Biological Transport, carbon, Catharanthus, Exocytosis, Fluorescence Recovery After Photobleaching, Fluorescent Dyes, I2CT, Intracellular Space, Nanotubes, Surface Properties, Team-Bianco, Vacuoles},
pubstate = {published},
tppubtype = {article}
}
As nanoparticles can cross different cellular barriers and access different tissues, control of their uptake and cellular fate presents a functional approach that will be broadly applicable to nanoscale technologies in cell biology. Here we show that the trafficking of single-walled carbon nanotubes (SWCNTs) through various subcellular membranes of the plant cell is facilitated or inhibited by attaching a suitable functional tag and controlling medium components. This enables a unique control over the uptake and the subcellular distribution of SWCNTs and provides a key strategy to promote their cellular elimination to minimize toxicity. Our results also demonstrate that SWCNTs are involved in a carrier-mediated transport (CMT) inside cells; this is a phenomenon that scientists could use to obtain novel molecular insights into CMT, with the potential translation to advances in subcellular nanobiology.
Ali-Boucetta Hanene, Al-Jamal Khuloud T, Müller Karin H, Li Shouping, Porter Alexandra E, Eddaoudi Ayad, Prato Maurizio, Bianco Alberto, Kostarelos Kostas
Cellular uptake and cytotoxic impact of chemically functionalized and polymer-coated carbon nanotubes Article de journal
Dans: Small (Weinheim an Der Bergstrasse, Germany), vol. 7, no. 22, p. 3230–3238, 2011, ISSN: 1613-6829.
Résumé | Liens | BibTeX | Étiquettes: Annexin A5, carbon, Cell Death, Cell Line, Endocytosis, Flow Cytometry, Fluorescein-5-isothiocyanate, Humans, I2CT, L-Lactate Dehydrogenase, mitochondria, Nanotubes, Polymers, Propidium, Surface Properties, Team-Bianco, tumor, water
@article{ali-boucetta_cellular_2011,
title = {Cellular uptake and cytotoxic impact of chemically functionalized and polymer-coated carbon nanotubes},
author = {Hanene Ali-Boucetta and Khuloud T Al-Jamal and Karin H Müller and Shouping Li and Alexandra E Porter and Ayad Eddaoudi and Maurizio Prato and Alberto Bianco and Kostas Kostarelos},
doi = {10.1002/smll.201101004},
issn = {1613-6829},
year = {2011},
date = {2011-11-01},
journal = {Small (Weinheim an Der Bergstrasse, Germany)},
volume = {7},
number = {22},
pages = {3230--3238},
abstract = {The impact of nanomaterials such as carbon nanotubes on biological matter is a topic of increasing interest and concern and requires a multifaceted approach to be resolved. A modified cytotoxic (lactate dehydrogenase (LDH)) assay is developed in an attempt to offer a valid and reliable methodology for screening carbon nanotube toxicity in vitro. Two of the most widely used types of surface-modified multiwalled carbon nanotubes (MWNTs) are tested: ammonium-functionalized MWNTs (MWNT-NH3+ ) and Pluronic F127 coated MWNTs (MWNT:F127). Chemically functionalized MWNTs show significantly greater cellular uptake into lung epithelial A549 cells compared to the non-covalently Pluronic F127-coated MWNTs. In spite of this, MWNT:F127 exhibit enhanced cytotoxicity according to the modified LDH assay. The validity of the modified LDH assay is further validated by direct comparison with other less reliable or accurate cytotoxicity assays. These findings indicate the reliability of the modified LDH assay as a screening tool to assess carbon nanotube cytotoxicity and illustrate that high levels of carbon nanotube cellular internalization do not necessarily lead to adverse responses.},
keywords = {Annexin A5, carbon, Cell Death, Cell Line, Endocytosis, Flow Cytometry, Fluorescein-5-isothiocyanate, Humans, I2CT, L-Lactate Dehydrogenase, mitochondria, Nanotubes, Polymers, Propidium, Surface Properties, Team-Bianco, tumor, water},
pubstate = {published},
tppubtype = {article}
}
The impact of nanomaterials such as carbon nanotubes on biological matter is a topic of increasing interest and concern and requires a multifaceted approach to be resolved. A modified cytotoxic (lactate dehydrogenase (LDH)) assay is developed in an attempt to offer a valid and reliable methodology for screening carbon nanotube toxicity in vitro. Two of the most widely used types of surface-modified multiwalled carbon nanotubes (MWNTs) are tested: ammonium-functionalized MWNTs (MWNT-NH3+ ) and Pluronic F127 coated MWNTs (MWNT:F127). Chemically functionalized MWNTs show significantly greater cellular uptake into lung epithelial A549 cells compared to the non-covalently Pluronic F127-coated MWNTs. In spite of this, MWNT:F127 exhibit enhanced cytotoxicity according to the modified LDH assay. The validity of the modified LDH assay is further validated by direct comparison with other less reliable or accurate cytotoxicity assays. These findings indicate the reliability of the modified LDH assay as a screening tool to assess carbon nanotube cytotoxicity and illustrate that high levels of carbon nanotube cellular internalization do not necessarily lead to adverse responses.
Montellano Alejandro, Ros Tatiana Da, Bianco Alberto, Prato Maurizio
Fullerene C₆₀ as a multifunctional system for drug and gene delivery Article de journal
Dans: Nanoscale, vol. 3, no. 10, p. 4035–4041, 2011, ISSN: 2040-3372.
Résumé | Liens | BibTeX | Étiquettes: DNA, Drug Carriers, Fullerenes, Gene Transfer Techniques, I2CT, Immunoconjugates, Plasmids, Team-Bianco
@article{montellano_fullerene_2011,
title = {Fullerene C₆₀ as a multifunctional system for drug and gene delivery},
author = {Alejandro Montellano and Tatiana Da Ros and Alberto Bianco and Maurizio Prato},
doi = {10.1039/c1nr10783f},
issn = {2040-3372},
year = {2011},
date = {2011-10-01},
journal = {Nanoscale},
volume = {3},
number = {10},
pages = {4035--4041},
abstract = {The fullerene family, and especially C(60), has delighted the scientific community during the last 25 years with perspective applications in a wide variety of fields, including the biological and the biomedical domains. Several biomedical uses have been explored using water-soluble C(60)-derivatives. However, the employment of fullerenes for drug delivery is still at an early stage of development. The design and synthesis of multifunctionalized and multimodal C(60) systems able to cross the cell membranes and efficiently deliver active molecules is an attracting challenge that involves multidisciplinary strategies. Promising results have emerged in the last years, bringing fullerenes again to the front of interest. Herein, the state of the art of this emerging field is presented and illustrated with some of the most representative examples.},
keywords = {DNA, Drug Carriers, Fullerenes, Gene Transfer Techniques, I2CT, Immunoconjugates, Plasmids, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
The fullerene family, and especially C(60), has delighted the scientific community during the last 25 years with perspective applications in a wide variety of fields, including the biological and the biomedical domains. Several biomedical uses have been explored using water-soluble C(60)-derivatives. However, the employment of fullerenes for drug delivery is still at an early stage of development. The design and synthesis of multifunctionalized and multimodal C(60) systems able to cross the cell membranes and efficiently deliver active molecules is an attracting challenge that involves multidisciplinary strategies. Promising results have emerged in the last years, bringing fullerenes again to the front of interest. Herein, the state of the art of this emerging field is presented and illustrated with some of the most representative examples.
Bianco Alberto, Kostarelos Kostas, Prato Maurizio
Making carbon nanotubes biocompatible and biodegradable Article de journal
Dans: Chemical Communications (Cambridge, England), vol. 47, no. 37, p. 10182–10188, 2011, ISSN: 1364-548X.
Résumé | Liens | BibTeX | Étiquettes: Animals, Biocompatible, carbon, Coated Materials, HeLa Cells, Humans, I2CT, nanotechnology, Nanotubes, Team-Bianco
@article{bianco_making_2011,
title = {Making carbon nanotubes biocompatible and biodegradable},
author = {Alberto Bianco and Kostas Kostarelos and Maurizio Prato},
doi = {10.1039/c1cc13011k},
issn = {1364-548X},
year = {2011},
date = {2011-10-01},
journal = {Chemical Communications (Cambridge, England)},
volume = {47},
number = {37},
pages = {10182--10188},
abstract = {Carbon nanotubes are promising nanomaterials with great potential in the field of nanomedicine for both therapeutic and diagnostic applications. Different approaches have been developed to render this material biocompatible and to modulate any ensuing toxic effects. In the context of medical use, although chemically functionalised carbon nanotubes display reduced toxicity, they are still considered with scepticism due to their perceived non-biodegradability. Recently, it has been demonstrated that functionalised carbon nanotubes can be degraded by oxidative enzymes. This finding is offering a new perspective for the development of carbon nanotubes in medicine. This article highlights recent advances that can act as paradigm-shifts towards the design of biocompatible and biodegradable functionalised carbon nanotubes and allow their translation into the clinic.},
keywords = {Animals, Biocompatible, carbon, Coated Materials, HeLa Cells, Humans, I2CT, nanotechnology, Nanotubes, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes are promising nanomaterials with great potential in the field of nanomedicine for both therapeutic and diagnostic applications. Different approaches have been developed to render this material biocompatible and to modulate any ensuing toxic effects. In the context of medical use, although chemically functionalised carbon nanotubes display reduced toxicity, they are still considered with scepticism due to their perceived non-biodegradability. Recently, it has been demonstrated that functionalised carbon nanotubes can be degraded by oxidative enzymes. This finding is offering a new perspective for the development of carbon nanotubes in medicine. This article highlights recent advances that can act as paradigm-shifts towards the design of biocompatible and biodegradable functionalised carbon nanotubes and allow their translation into the clinic.
Singh Prabhpreet, Lamanna Giuseppe, Ménard-Moyon Cécilia, Toma Francesca Maria, Magnano Elena, Bondino Federica, Prato Maurizio, Verma Sandeep, Bianco Alberto
Formation of efficient catalytic silver nanoparticles on carbon nanotubes by adenine functionalization Article de journal
Dans: Angewandte Chemie (International Ed. in English), vol. 50, no. 42, p. 9893–9897, 2011, ISSN: 1521-3773.
Résumé | Liens | BibTeX | Étiquettes: Adenine, carbon, Catalysis, I2CT, Metal Nanoparticles, Molecular Structure, Nanotubes, Silver, Surface Properties, Team-Bianco
@article{singh_formation_2011,
title = {Formation of efficient catalytic silver nanoparticles on carbon nanotubes by adenine functionalization},
author = {Prabhpreet Singh and Giuseppe Lamanna and Cécilia Ménard-Moyon and Francesca Maria Toma and Elena Magnano and Federica Bondino and Maurizio Prato and Sandeep Verma and Alberto Bianco},
doi = {10.1002/anie.201102976},
issn = {1521-3773},
year = {2011},
date = {2011-10-01},
journal = {Angewandte Chemie (International Ed. in English)},
volume = {50},
number = {42},
pages = {9893--9897},
abstract = {Stuck together: adenine/carbon nanotube hybrids trigger the formation of controlled-size catalytic silver nanoparticles on the nanotube surface. The catalytic efficiency of the resulting species was assessed in the oxidation of 2-methylhydroquinone to its corresponding benzoquinone, with complete recovery and without loss of activity of the catalyst.},
keywords = {Adenine, carbon, Catalysis, I2CT, Metal Nanoparticles, Molecular Structure, Nanotubes, Silver, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Stuck together: adenine/carbon nanotube hybrids trigger the formation of controlled-size catalytic silver nanoparticles on the nanotube surface. The catalytic efficiency of the resulting species was assessed in the oxidation of 2-methylhydroquinone to its corresponding benzoquinone, with complete recovery and without loss of activity of the catalyst.
Venturelli Enrica, Fabbro Chiara, Chaloin Olivier, Ménard-Moyon Cécilia, Smulski Cristian R, Ros Tatiana Da, Kostarelos Kostas, Prato Maurizio, Bianco Alberto
Antibody covalent immobilization on carbon nanotubes and assessment of antigen binding Article de journal
Dans: Small (Weinheim an Der Bergstrasse, Germany), vol. 7, no. 15, p. 2179–2187, 2011, ISSN: 1613-6829.
Résumé | Liens | BibTeX | Étiquettes: Antibodies, Antigens, carbon, I2CT, Immobilized, Mucin-1, nanotechnology, Nanotubes, Protein Binding, Team-Bianco, Thermogravimetry
@article{venturelli_antibody_2011,
title = {Antibody covalent immobilization on carbon nanotubes and assessment of antigen binding},
author = {Enrica Venturelli and Chiara Fabbro and Olivier Chaloin and Cécilia Ménard-Moyon and Cristian R Smulski and Tatiana Da Ros and Kostas Kostarelos and Maurizio Prato and Alberto Bianco},
doi = {10.1002/smll.201100137},
issn = {1613-6829},
year = {2011},
date = {2011-08-01},
journal = {Small (Weinheim an Der Bergstrasse, Germany)},
volume = {7},
number = {15},
pages = {2179--2187},
abstract = {Controlling the covalent bonding of antibodies onto functionalized carbon nanotubes is a key step in the design and preparation of nanotube-based conjugates for targeting cancer cells. For this purpose, an anti-MUC1 antibody (Ab) is linked to both multi-walled (MWCNTs) and double-walled carbon nanotubes (DWCNTs) using different synthetic strategies. The presence of the Ab attached to the nanotubes is confirmed by gel electrophoresis and thermogravimetric analysis. Most importantly, molecular recognition of the antigen by surface plasmon resonance is able to determine similar Ab binding capacities for both Ab-DWCNTs and Ab-MWCNTs. These results are very relevant for the design of future receptor-targeting strategies using chemically functionalized carbon nanotubes.},
keywords = {Antibodies, Antigens, carbon, I2CT, Immobilized, Mucin-1, nanotechnology, Nanotubes, Protein Binding, Team-Bianco, Thermogravimetry},
pubstate = {published},
tppubtype = {article}
}
Controlling the covalent bonding of antibodies onto functionalized carbon nanotubes is a key step in the design and preparation of nanotube-based conjugates for targeting cancer cells. For this purpose, an anti-MUC1 antibody (Ab) is linked to both multi-walled (MWCNTs) and double-walled carbon nanotubes (DWCNTs) using different synthetic strategies. The presence of the Ab attached to the nanotubes is confirmed by gel electrophoresis and thermogravimetric analysis. Most importantly, molecular recognition of the antigen by surface plasmon resonance is able to determine similar Ab binding capacities for both Ab-DWCNTs and Ab-MWCNTs. These results are very relevant for the design of future receptor-targeting strategies using chemically functionalized carbon nanotubes.
Lamanna Giuseppe, Russier Julie, Ménard-Moyon Cécilia, Bianco Alberto
HYDRAmers: design, synthesis and characterization of different generation novel Hydra-like dendrons based on multifunctionalized adamantane Article de journal
Dans: Chemical Communications (Cambridge, England), vol. 47, no. 31, p. 8955–8957, 2011, ISSN: 1364-548X.
Résumé | Liens | BibTeX | Étiquettes: Adamantane, Animals, Cell Line, Dendrimers, Drug Design, Humans, I2CT, L-Lactate Dehydrogenase, Magnetic Resonance Spectroscopy, Mice, Team-Bianco, tumor
@article{lamanna_hydramers_2011,
title = {HYDRAmers: design, synthesis and characterization of different generation novel Hydra-like dendrons based on multifunctionalized adamantane},
author = {Giuseppe Lamanna and Julie Russier and Cécilia Ménard-Moyon and Alberto Bianco},
doi = {10.1039/c1cc11689d},
issn = {1364-548X},
year = {2011},
date = {2011-08-01},
journal = {Chemical Communications (Cambridge, England)},
volume = {47},
number = {31},
pages = {8955--8957},
abstract = {In this communication we present a new synthetic strategy to different generation Hydra-like dendrons based on tetrafunctionalized adamantane as a building block. The novel dendrons, which we termed HYDRAmers, possess at the periphery and at the central core orthogonal protections that can be exploited for conjugation of targeting ligands, drugs and/or imaging probes.},
keywords = {Adamantane, Animals, Cell Line, Dendrimers, Drug Design, Humans, I2CT, L-Lactate Dehydrogenase, Magnetic Resonance Spectroscopy, Mice, Team-Bianco, tumor},
pubstate = {published},
tppubtype = {article}
}
In this communication we present a new synthetic strategy to different generation Hydra-like dendrons based on tetrafunctionalized adamantane as a building block. The novel dendrons, which we termed HYDRAmers, possess at the periphery and at the central core orthogonal protections that can be exploited for conjugation of targeting ligands, drugs and/or imaging probes.
Al-Jamal Khuloud T, Gherardini Lisa, Bardi Giuseppe, Nunes Antonio, Guo Chang, Bussy Cyrill, Herrero Antonia M, Bianco Alberto, Prato Maurizio, Kostarelos Kostas, Pizzorusso Tommaso
Functional motor recovery from brain ischemic insult by carbon nanotube-mediated siRNA silencing Article de journal
Dans: Proceedings of the National Academy of Sciences of the United States of America, vol. 108, no. 27, p. 10952–10957, 2011, ISSN: 1091-6490.
Résumé | Liens | BibTeX | Étiquettes: Animals, Apoptosis, Base Sequence, Brain Ischemia, carbon, Caspase 3, Caspase Inhibitors, Cell Line, Cells, Cultured, Electron, Endothelin-1, Female, Genetic Therapy, I2CT, Inbred C57BL, Mice, Microscopy, Nanomedicine, Nanotubes, Neurons, Psychomotor Performance, Rats, RNA, RNA Interference, Small Interfering, Sprague-Dawley, Team-Bianco, Transmission
@article{al-jamal_functional_2011,
title = {Functional motor recovery from brain ischemic insult by carbon nanotube-mediated siRNA silencing},
author = {Khuloud T Al-Jamal and Lisa Gherardini and Giuseppe Bardi and Antonio Nunes and Chang Guo and Cyrill Bussy and Antonia M Herrero and Alberto Bianco and Maurizio Prato and Kostas Kostarelos and Tommaso Pizzorusso},
doi = {10.1073/pnas.1100930108},
issn = {1091-6490},
year = {2011},
date = {2011-07-01},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {108},
number = {27},
pages = {10952--10957},
abstract = {Stroke is the second cause of death worldwide with ischemic stroke accounting for 80% of all stroke insults. Caspase-3 activation contributes to brain tissue loss and downstream biochemical events that lead to programmed cell death after traumatic brain injury. Alleviation of symptoms following ischemic neuronal injury can be potentially achieved by either genetic disruption or pharmacological inhibition of caspases. Here, we studied whether silencing of Caspase-3 using carbon nanotube-mediated in vivo RNA interference (RNAi) could offer a therapeutic opportunity against stroke. Effective delivery of siRNA directly to the CNS has been shown to normalize phenotypes in animal models of several neurological diseases. It is shown here that peri-lesional stereotactic administration of a Caspase-3 siRNA (siCas 3) delivered by functionalized carbon nanotubes (f-CNT) reduced neurodegeneration and promoted functional preservation before and after focal ischemic damage of the rodent motor cortex using an endothelin-1 induced stroke model. These observations illustrate the opportunity offered by carbon nanotube-mediated siRNA delivery and gene silencing of neuronal tissue applicable to a variety of different neuropathological conditions where intervention at well localized brain foci may offer therapeutic and functional benefits.},
keywords = {Animals, Apoptosis, Base Sequence, Brain Ischemia, carbon, Caspase 3, Caspase Inhibitors, Cell Line, Cells, Cultured, Electron, Endothelin-1, Female, Genetic Therapy, I2CT, Inbred C57BL, Mice, Microscopy, Nanomedicine, Nanotubes, Neurons, Psychomotor Performance, Rats, RNA, RNA Interference, Small Interfering, Sprague-Dawley, Team-Bianco, Transmission},
pubstate = {published},
tppubtype = {article}
}
Stroke is the second cause of death worldwide with ischemic stroke accounting for 80% of all stroke insults. Caspase-3 activation contributes to brain tissue loss and downstream biochemical events that lead to programmed cell death after traumatic brain injury. Alleviation of symptoms following ischemic neuronal injury can be potentially achieved by either genetic disruption or pharmacological inhibition of caspases. Here, we studied whether silencing of Caspase-3 using carbon nanotube-mediated in vivo RNA interference (RNAi) could offer a therapeutic opportunity against stroke. Effective delivery of siRNA directly to the CNS has been shown to normalize phenotypes in animal models of several neurological diseases. It is shown here that peri-lesional stereotactic administration of a Caspase-3 siRNA (siCas 3) delivered by functionalized carbon nanotubes (f-CNT) reduced neurodegeneration and promoted functional preservation before and after focal ischemic damage of the rodent motor cortex using an endothelin-1 induced stroke model. These observations illustrate the opportunity offered by carbon nanotube-mediated siRNA delivery and gene silencing of neuronal tissue applicable to a variety of different neuropathological conditions where intervention at well localized brain foci may offer therapeutic and functional benefits.
Singh Prabhpreet, Toma Francesca Maria, Kumar Jitendra, Venkatesh V, Raya Jesus, Prato Maurizio, Verma Sandeep, Bianco Alberto
Carbon nanotube-nucleobase hybrids: nanorings from uracil-modified single-walled carbon nanotubes Article de journal
Dans: Chemistry (Weinheim an Der Bergstrasse, Germany), vol. 17, no. 24, p. 6772–6780, 2011, ISSN: 1521-3765.
Résumé | Liens | BibTeX | Étiquettes: carbon, I2CT, Magnetic Resonance Spectroscopy, Molecular Structure, Nanotubes, Team-Bianco, Uracil
@article{singh_carbon_2011,
title = {Carbon nanotube-nucleobase hybrids: nanorings from uracil-modified single-walled carbon nanotubes},
author = {Prabhpreet Singh and Francesca Maria Toma and Jitendra Kumar and V Venkatesh and Jesus Raya and Maurizio Prato and Sandeep Verma and Alberto Bianco},
doi = {10.1002/chem.201100312},
issn = {1521-3765},
year = {2011},
date = {2011-06-01},
journal = {Chemistry (Weinheim an Der Bergstrasse, Germany)},
volume = {17},
number = {24},
pages = {6772--6780},
abstract = {Single-walled carbon nanotubes (SWCNTs) have been covalently functionalized with uracil nucleobase. The hybrids have been characterized by using complementary spectroscopic and microscopic techniques including solid-state NMR spectroscopy. The uracil-functionalized SWCNTs are able to self-assemble into regular nanorings with a diameter of 50-70 nm, as observed by AFM and TEM. AFM shows that the rings do not have a consistent height and thickness, which indicates that they may be formed by separate bundles of CNTs. The simplest model for the nanoring formation likely involves two bundles of CNTs interacting with each other via uracil-uracil base-pairing at both CNT ends. These nanorings can be envisaged for the development of advanced electronic circuits.},
keywords = {carbon, I2CT, Magnetic Resonance Spectroscopy, Molecular Structure, Nanotubes, Team-Bianco, Uracil},
pubstate = {published},
tppubtype = {article}
}
Single-walled carbon nanotubes (SWCNTs) have been covalently functionalized with uracil nucleobase. The hybrids have been characterized by using complementary spectroscopic and microscopic techniques including solid-state NMR spectroscopy. The uracil-functionalized SWCNTs are able to self-assemble into regular nanorings with a diameter of 50-70 nm, as observed by AFM and TEM. AFM shows that the rings do not have a consistent height and thickness, which indicates that they may be formed by separate bundles of CNTs. The simplest model for the nanoring formation likely involves two bundles of CNTs interacting with each other via uracil-uracil base-pairing at both CNT ends. These nanorings can be envisaged for the development of advanced electronic circuits.
Murphy Fiona A, Poland Craig A, Duffin Rodger, Al-Jamal Khuloud T, Ali-Boucetta Hanene, Nunes Antonio, Byrne Fiona, Prina-Mello Adriele, Volkov Yuri, Li Shouping, Mather Stephen J, Bianco Alberto, Prato Maurizio, Macnee William, Wallace William A, Kostarelos Kostas, Donaldson Ken
Length-dependent retention of carbon nanotubes in the pleural space of mice initiates sustained inflammation and progressive fibrosis on the parietal pleura Article de journal
Dans: The American Journal of Pathology, vol. 178, no. 6, p. 2587–2600, 2011, ISSN: 1525-2191.
Résumé | Liens | BibTeX | Étiquettes: Animals, carbon, Cell Proliferation, Disease Progression, Emission-Computed, Epithelium, Fibrosis, I2CT, inflammation, Lymph Nodes, Mediastinum, Mice, Nanotubes, Nanowires, Particle Size, Pleura, Pleural Cavity, Single-Photon, Team-Bianco, Time Factors, Tomography, X-Ray Computed
@article{murphy_length-dependent_2011,
title = {Length-dependent retention of carbon nanotubes in the pleural space of mice initiates sustained inflammation and progressive fibrosis on the parietal pleura},
author = {Fiona A Murphy and Craig A Poland and Rodger Duffin and Khuloud T Al-Jamal and Hanene Ali-Boucetta and Antonio Nunes and Fiona Byrne and Adriele Prina-Mello and Yuri Volkov and Shouping Li and Stephen J Mather and Alberto Bianco and Maurizio Prato and William Macnee and William A Wallace and Kostas Kostarelos and Ken Donaldson},
doi = {10.1016/j.ajpath.2011.02.040},
issn = {1525-2191},
year = {2011},
date = {2011-06-01},
journal = {The American Journal of Pathology},
volume = {178},
number = {6},
pages = {2587--2600},
abstract = {The fibrous shape of carbon nanotubes (CNTs) raises concern that they may pose an asbestos-like inhalation hazard, leading to the development of diseases, especially mesothelioma. Direct instillation of long and short CNTs into the pleural cavity, the site of mesothelioma development, produced asbestos-like length-dependent responses. The response to long CNTs and long asbestos was characterized by acute inflammation, leading to progressive fibrosis on the parietal pleura, where stomata of strictly defined size limit the egress of long, but not short, fibers. This was confirmed by demonstrating clearance of short, but not long, CNT and nickel nanowires and by visualizing the migration of short CNTs from the pleural space by single-photon emission computed tomographic imaging. Our data confirm the hypothesis that, although a proportion of all deposited particles passes through the pleura, the pathogenicity of long CNTs and other fibers arises as a result of length-dependent retention at the stomata on the parietal pleura.},
keywords = {Animals, carbon, Cell Proliferation, Disease Progression, Emission-Computed, Epithelium, Fibrosis, I2CT, inflammation, Lymph Nodes, Mediastinum, Mice, Nanotubes, Nanowires, Particle Size, Pleura, Pleural Cavity, Single-Photon, Team-Bianco, Time Factors, Tomography, X-Ray Computed},
pubstate = {published},
tppubtype = {article}
}
The fibrous shape of carbon nanotubes (CNTs) raises concern that they may pose an asbestos-like inhalation hazard, leading to the development of diseases, especially mesothelioma. Direct instillation of long and short CNTs into the pleural cavity, the site of mesothelioma development, produced asbestos-like length-dependent responses. The response to long CNTs and long asbestos was characterized by acute inflammation, leading to progressive fibrosis on the parietal pleura, where stomata of strictly defined size limit the egress of long, but not short, fibers. This was confirmed by demonstrating clearance of short, but not long, CNT and nickel nanowires and by visualizing the migration of short CNTs from the pleural space by single-photon emission computed tomographic imaging. Our data confirm the hypothesis that, although a proportion of all deposited particles passes through the pleura, the pathogenicity of long CNTs and other fibers arises as a result of length-dependent retention at the stomata on the parietal pleura.
Al-Jamal Khuloud T, Nerl Hannah, Müller Karin H, Ali-Boucetta Hanene, Li Shouping, Haynes Peter D, Jinschek Joerg R, Prato Maurizio, Bianco Alberto, Kostarelos Kostas, Porter Alexandra E
Cellular uptake mechanisms of functionalised multi-walled carbon nanotubes by 3D electron tomography imaging Article de journal
Dans: Nanoscale, vol. 3, no. 6, p. 2627–2635, 2011, ISSN: 2040-3372.
Résumé | Liens | BibTeX | Étiquettes: carbon, Cell Line, Cell Membrane, Cytoplasm, Electron Microscope Tomography, Humans, I2CT, imaging, Macrophages, Nanotubes, Phagocytosis, Phagosomes, Team-Bianco, Three-Dimensional, tumor
@article{al-jamal_cellular_2011,
title = {Cellular uptake mechanisms of functionalised multi-walled carbon nanotubes by 3D electron tomography imaging},
author = {Khuloud T Al-Jamal and Hannah Nerl and Karin H Müller and Hanene Ali-Boucetta and Shouping Li and Peter D Haynes and Joerg R Jinschek and Maurizio Prato and Alberto Bianco and Kostas Kostarelos and Alexandra E Porter},
doi = {10.1039/c1nr10080g},
issn = {2040-3372},
year = {2011},
date = {2011-06-01},
journal = {Nanoscale},
volume = {3},
number = {6},
pages = {2627--2635},
abstract = {Carbon nanotubes (CNTs) are being investigated for a variety of biomedical applications. Despite numerous studies, the pathways by which carbon nanotubes enter cells and their subsequent intracellular trafficking and distribution remain poorly determined. Here, we use 3-D electron tomography techniques that offer optimum enhancement of contrast between carbon nanotubes and the plasma membrane to investigate the mechanisms involved in the cellular uptake of shortened, functionalised multi-walled carbon nanotubes (MWNT-NH(3)(+)). Both human lung epithelial (A549) cells, that are almost incapable of phagocytosis and primary macrophages, capable of extremely efficient phagocytosis, were used. We observed that MWNT-NH(3)(+) were internalised in both phagocytic and non-phagocytic cells by any one of three mechanisms: (a) individually via membrane wrapping; (b) individually by direct membrane translocation; and (c) in clusters within vesicular compartments. At early time points following intracellular translocation, we noticed accumulation of nanotube material within various intracellular compartments, while a long-term (14-day) study using primary human macrophages revealed that MWNT-NH(3)(+) were able to escape vesicular (phagosome) entrapment by translocating directly into the cytoplasm.},
keywords = {carbon, Cell Line, Cell Membrane, Cytoplasm, Electron Microscope Tomography, Humans, I2CT, imaging, Macrophages, Nanotubes, Phagocytosis, Phagosomes, Team-Bianco, Three-Dimensional, tumor},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes (CNTs) are being investigated for a variety of biomedical applications. Despite numerous studies, the pathways by which carbon nanotubes enter cells and their subsequent intracellular trafficking and distribution remain poorly determined. Here, we use 3-D electron tomography techniques that offer optimum enhancement of contrast between carbon nanotubes and the plasma membrane to investigate the mechanisms involved in the cellular uptake of shortened, functionalised multi-walled carbon nanotubes (MWNT-NH(3)(+)). Both human lung epithelial (A549) cells, that are almost incapable of phagocytosis and primary macrophages, capable of extremely efficient phagocytosis, were used. We observed that MWNT-NH(3)(+) were internalised in both phagocytic and non-phagocytic cells by any one of three mechanisms: (a) individually via membrane wrapping; (b) individually by direct membrane translocation; and (c) in clusters within vesicular compartments. At early time points following intracellular translocation, we noticed accumulation of nanotube material within various intracellular compartments, while a long-term (14-day) study using primary human macrophages revealed that MWNT-NH(3)(+) were able to escape vesicular (phagosome) entrapment by translocating directly into the cytoplasm.
Russier Julie, Ménard-Moyon Cécilia, Venturelli Enrica, Gravel Edmond, Marcolongo Gabriele, Meneghetti Moreno, Doris Eric, Bianco Alberto
Oxidative biodegradation of single- and multi-walled carbon nanotubes Article de journal
Dans: Nanoscale, vol. 3, no. 3, p. 893–896, 2011, ISSN: 2040-3372.
Résumé | Liens | BibTeX | Étiquettes: Absorbable Implants, Biocompatible Materials, Body Fluids, carbon, Horseradish Peroxidase, Hydrogen Peroxide, I2CT, Macromolecular Substances, Materials Testing, Molecular Conformation, Nanotubes, Oxidation-Reduction, Particle Size, Surface Properties, Team-Bianco
@article{russier_oxidative_2011,
title = {Oxidative biodegradation of single- and multi-walled carbon nanotubes},
author = {Julie Russier and Cécilia Ménard-Moyon and Enrica Venturelli and Edmond Gravel and Gabriele Marcolongo and Moreno Meneghetti and Eric Doris and Alberto Bianco},
doi = {10.1039/c0nr00779j},
issn = {2040-3372},
year = {2011},
date = {2011-03-01},
journal = {Nanoscale},
volume = {3},
number = {3},
pages = {893--896},
abstract = {In this study we compare the biodegradation of both single-walled (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) using two different oxidative conditions. In particular, we demonstrate that oxidized multi-walled carbon nanotubes are highly degraded, although not to completeness when treated with horseradish peroxidase (HRP) in the presence of hydrogen peroxide.},
keywords = {Absorbable Implants, Biocompatible Materials, Body Fluids, carbon, Horseradish Peroxidase, Hydrogen Peroxide, I2CT, Macromolecular Substances, Materials Testing, Molecular Conformation, Nanotubes, Oxidation-Reduction, Particle Size, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
In this study we compare the biodegradation of both single-walled (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) using two different oxidative conditions. In particular, we demonstrate that oxidized multi-walled carbon nanotubes are highly degraded, although not to completeness when treated with horseradish peroxidase (HRP) in the presence of hydrogen peroxide.
Ménard-Moyon Cécilia, Fabbro Chiara, Prato Maurizio, Bianco Alberto
One-pot triple functionalization of carbon nanotubes Article de journal
Dans: Chemistry (Weinheim an Der Bergstrasse, Germany), vol. 17, no. 11, p. 3222–3227, 2011, ISSN: 1521-3765.
Résumé | Liens | BibTeX | Étiquettes: Aniline Compounds, Azo Compounds, Benzylamines, carbon, I2CT, Nanotubes, Raman, Spectrophotometry, Spectrum Analysis, Surface Properties, Team-Bianco
@article{menard-moyon_one-pot_2011,
title = {One-pot triple functionalization of carbon nanotubes},
author = {Cécilia Ménard-Moyon and Chiara Fabbro and Maurizio Prato and Alberto Bianco},
doi = {10.1002/chem.201003050},
issn = {1521-3765},
year = {2011},
date = {2011-03-01},
journal = {Chemistry (Weinheim an Der Bergstrasse, Germany)},
volume = {17},
number = {11},
pages = {3222--3227},
abstract = {Carbon nanotubes (CNTs) are very promising as carriers for the delivery of bioactive molecules. The multifunctionalization of CNTs is necessary to impart multimodalities for the development of future CNT-based multipotent therapeutic constructs. In this context, we report the first example of covalent trifunctionalization of different types of CNTs. Our strategy is a simple and efficient methodology based on the simultaneous functionalization of the nanotube surface with three different active groups. The reaction is performed in one step by arylation with diazonium salts generated in situ. The CNTs are functionalized with benzylamine moieties blocked with three different protecting groups that can be selectively removed under specific conditions. The trifunctionalized CNTs were characterized by TEM, thermogravimetric analysis, and Raman and UV/Vis/NIR spectroscopy, while the amine loading was determined by using the Kaiser test. The sequential removal of the protecting groups of the amine functions allows the grafting of the molecules of interest on the nanotube surface to be controlled.},
keywords = {Aniline Compounds, Azo Compounds, Benzylamines, carbon, I2CT, Nanotubes, Raman, Spectrophotometry, Spectrum Analysis, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes (CNTs) are very promising as carriers for the delivery of bioactive molecules. The multifunctionalization of CNTs is necessary to impart multimodalities for the development of future CNT-based multipotent therapeutic constructs. In this context, we report the first example of covalent trifunctionalization of different types of CNTs. Our strategy is a simple and efficient methodology based on the simultaneous functionalization of the nanotube surface with three different active groups. The reaction is performed in one step by arylation with diazonium salts generated in situ. The CNTs are functionalized with benzylamine moieties blocked with three different protecting groups that can be selectively removed under specific conditions. The trifunctionalized CNTs were characterized by TEM, thermogravimetric analysis, and Raman and UV/Vis/NIR spectroscopy, while the amine loading was determined by using the Kaiser test. The sequential removal of the protecting groups of the amine functions allows the grafting of the molecules of interest on the nanotube surface to be controlled.
Gaillard Claire, Duval Monique, Dumortier Hélène, Bianco Alberto
Carbon nanotube-coupled cell adhesion peptides are non-immunogenic: a promising step toward new biomedical devices Article de journal
Dans: Journal of Peptide Science: An Official Publication of the European Peptide Society, vol. 17, no. 2, p. 139–142, 2011, ISSN: 1099-1387.
Résumé | Liens | BibTeX | Étiquettes: carbon, Dumortier, Enzyme-Linked Immunosorbent Assay, I2CT, Nanotubes, Peptides, Team-Bianco, Team-Dumortier
@article{gaillard_carbon_2011,
title = {Carbon nanotube-coupled cell adhesion peptides are non-immunogenic: a promising step toward new biomedical devices},
author = {Claire Gaillard and Monique Duval and Hélène Dumortier and Alberto Bianco},
doi = {10.1002/psc.1290},
issn = {1099-1387},
year = {2011},
date = {2011-02-01},
journal = {Journal of Peptide Science: An Official Publication of the European Peptide Society},
volume = {17},
number = {2},
pages = {139--142},
abstract = {Carbon nanotubes functionalized with cell adhesion peptides can be considered as novel, promising candidates for the development of advanced drug delivery systems or for designing new generation of self-assembling nerve 'bridges'. An important step toward the integration of these types of conjugates in living bodies is the assessment of their impact on the immune system. In this direction, an integrin-derived peptide has been covalently conjugated to carbon nanotubes. Following intraperitoneal administration, peptide-carbon nanotubes do not trigger an anti-peptide antibody production. Demonstration of the immune neutrality of peptide-carbon nanotubes reinforces their potential use as substrates for neuronal regeneration in vivo.},
keywords = {carbon, Dumortier, Enzyme-Linked Immunosorbent Assay, I2CT, Nanotubes, Peptides, Team-Bianco, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}
Carbon nanotubes functionalized with cell adhesion peptides can be considered as novel, promising candidates for the development of advanced drug delivery systems or for designing new generation of self-assembling nerve 'bridges'. An important step toward the integration of these types of conjugates in living bodies is the assessment of their impact on the immune system. In this direction, an integrin-derived peptide has been covalently conjugated to carbon nanotubes. Following intraperitoneal administration, peptide-carbon nanotubes do not trigger an anti-peptide antibody production. Demonstration of the immune neutrality of peptide-carbon nanotubes reinforces their potential use as substrates for neuronal regeneration in vivo.
Serag Maged F, Kaji Noritada, Gaillard Claire, Okamoto Yukihiro, Terasaka Kazuyoshi, Jabasini Mohammad, Tokeshi Manabu, Mizukami Hajime, Bianco Alberto, Baba Yoshinobu
Trafficking and subcellular localization of multiwalled carbon nanotubes in plant cells Article de journal
Dans: ACS nano, vol. 5, no. 1, p. 493–499, 2011, ISSN: 1936-086X.
Résumé | Liens | BibTeX | Étiquettes: Biological Transport, carbon, Catharanthus, Cell Membrane, Endosomes, I2CT, Intracellular Space, Nanotubes, Protoplasts, Team-Bianco
@article{serag_trafficking_2011,
title = {Trafficking and subcellular localization of multiwalled carbon nanotubes in plant cells},
author = {Maged F Serag and Noritada Kaji and Claire Gaillard and Yukihiro Okamoto and Kazuyoshi Terasaka and Mohammad Jabasini and Manabu Tokeshi and Hajime Mizukami and Alberto Bianco and Yoshinobu Baba},
doi = {10.1021/nn102344t},
issn = {1936-086X},
year = {2011},
date = {2011-01-01},
journal = {ACS nano},
volume = {5},
number = {1},
pages = {493--499},
abstract = {Major barriers to delivery of biomolecules are crossing the cellular membranes and achieving a high cytoplasmic concentration by circumventing entrapment into endosomes and other lytic organelles. Motivated by such aim, we have investigated the capability of multiwalled carbon nanotubes (MWCNTs) to penetrate the cell membrane of plant protoplasts (plant cells made devoid of their cell walls via enzymatic treatment) and studied their internalization mechanism via confocal imaging and TEM techniques. Our results indentified an endosome-escaping uptake mode of MWCNTs by plant protoplasts. Moreover, short MWCNTs (textbackslashtextless100 nm) were observed to target specific cellular substructures including the nucleus, plastids, and vacuoles. These findings are expected to have a significant impact on plant cell biology and transformation technologies.},
keywords = {Biological Transport, carbon, Catharanthus, Cell Membrane, Endosomes, I2CT, Intracellular Space, Nanotubes, Protoplasts, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Major barriers to delivery of biomolecules are crossing the cellular membranes and achieving a high cytoplasmic concentration by circumventing entrapment into endosomes and other lytic organelles. Motivated by such aim, we have investigated the capability of multiwalled carbon nanotubes (MWCNTs) to penetrate the cell membrane of plant protoplasts (plant cells made devoid of their cell walls via enzymatic treatment) and studied their internalization mechanism via confocal imaging and TEM techniques. Our results indentified an endosome-escaping uptake mode of MWCNTs by plant protoplasts. Moreover, short MWCNTs (textbackslashtextless100 nm) were observed to target specific cellular substructures including the nucleus, plastids, and vacuoles. These findings are expected to have a significant impact on plant cell biology and transformation technologies.
Benincasa Monica, Pacor Sabrina, Wu Wei, Prato Maurizio, Bianco Alberto, Gennaro Renato
Antifungal activity of amphotericin B conjugated to carbon nanotubes Article de journal
Dans: ACS nano, vol. 5, no. 1, p. 199–208, 2011, ISSN: 1936-086X.
Résumé | Liens | BibTeX | Étiquettes: Amphotericin B, Antifungal Agents, Candida, carbon, Cell Membrane, Deoxycholic Acid, Drug Design, Drug Resistance, Fungal, Humans, I2CT, Jurkat Cells, Kinetics, Membrane Potentials, Nanotubes, Team-Bianco
@article{benincasa_antifungal_2011,
title = {Antifungal activity of amphotericin B conjugated to carbon nanotubes},
author = {Monica Benincasa and Sabrina Pacor and Wei Wu and Maurizio Prato and Alberto Bianco and Renato Gennaro},
doi = {10.1021/nn1023522},
issn = {1936-086X},
year = {2011},
date = {2011-01-01},
journal = {ACS nano},
volume = {5},
number = {1},
pages = {199--208},
abstract = {Amphotericin B (AMB) has long been considered the most effective drug in the treatment of serious invasive fungal infections. There are, however, major limitations to its use, due to several adverse effects, including acute infusional reactions and, most relevant, a dose-dependent nephrotoxicity. At least some of these effects are attributed to the aggregation of AMB as a result of its poor water solubility. To overcome this problem, reformulated versions of the drug have been developed, including a micellar dispersion of AMB with sodium deoxycholate (AMBD), its encapsulation into liposomes, or its incorporation into lipidic complexes. The development of nanobiotechnologies provides novel potential drug delivery systems that make use of nanomaterials such as functionalized carbon nanotubes (f-CNTs), which are emerging as an innovative and efficient tool for the transport and cellular translocation of therapeutic molecules. In this study, we prepared two conjugates between f-CNTs and AMB. The antifungal activity of these conjugates was tested against a collection of reference and clinical fungal strains, in comparison to that of AMB alone or AMBD. Measured minimum inhibition concentration (MIC) values for f-CNT-AMB conjugates were either comparable to or better than those displayed by AMB and AMBD. Furthermore, AMBD-resistant Candida strains were found to be susceptible to f-CNT-AMB 1. Additional studies, aimed at understanding the mechanism of action of the conjugates, suggest a nonlytic mechanism, since the compounds show a major permeabilizing effect on the tested fungal strains only after extended incubation. Interestingly, the f-CNT-AMB 1 does not show any significant toxic effect on Jurkat cells at antifungal concentrations.},
keywords = {Amphotericin B, Antifungal Agents, Candida, carbon, Cell Membrane, Deoxycholic Acid, Drug Design, Drug Resistance, Fungal, Humans, I2CT, Jurkat Cells, Kinetics, Membrane Potentials, Nanotubes, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Amphotericin B (AMB) has long been considered the most effective drug in the treatment of serious invasive fungal infections. There are, however, major limitations to its use, due to several adverse effects, including acute infusional reactions and, most relevant, a dose-dependent nephrotoxicity. At least some of these effects are attributed to the aggregation of AMB as a result of its poor water solubility. To overcome this problem, reformulated versions of the drug have been developed, including a micellar dispersion of AMB with sodium deoxycholate (AMBD), its encapsulation into liposomes, or its incorporation into lipidic complexes. The development of nanobiotechnologies provides novel potential drug delivery systems that make use of nanomaterials such as functionalized carbon nanotubes (f-CNTs), which are emerging as an innovative and efficient tool for the transport and cellular translocation of therapeutic molecules. In this study, we prepared two conjugates between f-CNTs and AMB. The antifungal activity of these conjugates was tested against a collection of reference and clinical fungal strains, in comparison to that of AMB alone or AMBD. Measured minimum inhibition concentration (MIC) values for f-CNT-AMB conjugates were either comparable to or better than those displayed by AMB and AMBD. Furthermore, AMBD-resistant Candida strains were found to be susceptible to f-CNT-AMB 1. Additional studies, aimed at understanding the mechanism of action of the conjugates, suggest a nonlytic mechanism, since the compounds show a major permeabilizing effect on the tested fungal strains only after extended incubation. Interestingly, the f-CNT-AMB 1 does not show any significant toxic effect on Jurkat cells at antifungal concentrations.
Herrero Antonia M, Lacerda Lara, Bianco Alberto, Kostarelos Kostas, Prato Maurizio
Functionalised carbon nanotubes: high biocompatibility with lack of toxicity Article de journal
Dans: International Journal of Nanotechnology, vol. 8, no. 10/11/12, p. 885, 2011, ISSN: 1475-7435, 1741-8151.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{herrero_functionalised_2011,
title = {Functionalised carbon nanotubes: high biocompatibility with lack of toxicity},
author = {Antonia M Herrero and Lara Lacerda and Alberto Bianco and Kostas Kostarelos and Maurizio Prato},
url = {http://www.inderscience.com/link.php?id=44433},
doi = {10.1504/IJNT.2011.044433},
issn = {1475-7435, 1741-8151},
year = {2011},
date = {2011-01-01},
urldate = {2020-04-01},
journal = {International Journal of Nanotechnology},
volume = {8},
number = {10/11/12},
pages = {885},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
2010
Cellot Giada, Ballerini Laura, Prato Maurizio, Bianco Alberto
Neurons are able to internalize soluble carbon nanotubes: new opportunities or old risks? Article de journal
Dans: Small (Weinheim an Der Bergstrasse, Germany), vol. 6, no. 23, p. 2630–2633, 2010, ISSN: 1613-6829.
Liens | BibTeX | Étiquettes: carbon, Cell Line, Cells, Cultured, Humans, I2CT, Nanotubes, Neurons, Team-Bianco, tumor
@article{cellot_neurons_2010,
title = {Neurons are able to internalize soluble carbon nanotubes: new opportunities or old risks?},
author = {Giada Cellot and Laura Ballerini and Maurizio Prato and Alberto Bianco},
doi = {10.1002/smll.201000906},
issn = {1613-6829},
year = {2010},
date = {2010-12-01},
journal = {Small (Weinheim an Der Bergstrasse, Germany)},
volume = {6},
number = {23},
pages = {2630--2633},
keywords = {carbon, Cell Line, Cells, Cultured, Humans, I2CT, Nanotubes, Neurons, Team-Bianco, tumor},
pubstate = {published},
tppubtype = {article}
}
Al-Jamal Khuloud T, Toma Francesca M, Yilmazer Açelya, Ali-Boucetta Hanene, Nunes Antonio, Herrero Maria-Antonia, Tian Bowen, Eddaoudi Ayad, Eddaoui Ayad, Al-Jamal Wafa' T, Bianco Alberto, Prato Maurizio, Kostarelo Kostas
Enhanced cellular internalization and gene silencing with a series of cationic dendron-multiwalled carbon nanotube:siRNA complexes Article de journal
Dans: FASEB journal: official publication of the Federation of American Societies for Experimental Biology, vol. 24, no. 11, p. 4354–4365, 2010, ISSN: 1530-6860.
Résumé | Liens | BibTeX | Étiquettes: Biological Transport, carbon, Cations, Cell Line, Cell Survival, Gene Silencing, HeLa Cells, Humans, I2CT, Models, Molecular, Nanotubes, RNA, Small Interfering, Team-Bianco, Transfection, tumor
@article{al-jamal_enhanced_2010,
title = {Enhanced cellular internalization and gene silencing with a series of cationic dendron-multiwalled carbon nanotube:siRNA complexes},
author = {Khuloud T Al-Jamal and Francesca M Toma and Açelya Yilmazer and Hanene Ali-Boucetta and Antonio Nunes and Maria-Antonia Herrero and Bowen Tian and Ayad Eddaoudi and Ayad Eddaoui and Wafa' T Al-Jamal and Alberto Bianco and Maurizio Prato and Kostas Kostarelo},
doi = {10.1096/fj.09-141036},
issn = {1530-6860},
year = {2010},
date = {2010-11-01},
journal = {FASEB journal: official publication of the Federation of American Societies for Experimental Biology},
volume = {24},
number = {11},
pages = {4354--4365},
abstract = {One of the major obstacles to the clinical development of gene silencing by small interfering RNA (siRNA) is its effective cytoplasmic delivery. Carbon nanotubes have been proposed as novel nanomaterials that can offer significant advantages for the intracellular delivery of nucleic acids, such as siRNA. We recently demonstrated in a proof-of-principle study that amino-functionalized multiwalled carbon nanotubes (f-MWNT) can effectively deliver in vivo an siRNA sequence, triggering cell apoptosis that results in human lung xenograft eradication and prolonged survival. In the present study, we demonstrate how a newly synthesized series of polycationic dendron-MWNT constructs with a precisely tailored number of amino functions (dendron generations) can complex and effectively deliver double-stranded siRNA to achieve gene silencing in vitro. A systematic comparison between the f-MWNT series in terms of cellular uptake, cytotoxicity, and siRNA complexation is offered. Significant improvement in siRNA delivery with the dendron-MWNT conjugates is shown, and gene silencing was obtained in 2 human cell lines using 2 different siRNA sequences. The study reveals that through f-MWNT structure-biological function analysis novel nanotube-based siRNA transfer vectors can be designed with minimal cytotoxicity and effective delivery and gene-silencing capabilities.},
keywords = {Biological Transport, carbon, Cations, Cell Line, Cell Survival, Gene Silencing, HeLa Cells, Humans, I2CT, Models, Molecular, Nanotubes, RNA, Small Interfering, Team-Bianco, Transfection, tumor},
pubstate = {published},
tppubtype = {article}
}
One of the major obstacles to the clinical development of gene silencing by small interfering RNA (siRNA) is its effective cytoplasmic delivery. Carbon nanotubes have been proposed as novel nanomaterials that can offer significant advantages for the intracellular delivery of nucleic acids, such as siRNA. We recently demonstrated in a proof-of-principle study that amino-functionalized multiwalled carbon nanotubes (f-MWNT) can effectively deliver in vivo an siRNA sequence, triggering cell apoptosis that results in human lung xenograft eradication and prolonged survival. In the present study, we demonstrate how a newly synthesized series of polycationic dendron-MWNT constructs with a precisely tailored number of amino functions (dendron generations) can complex and effectively deliver double-stranded siRNA to achieve gene silencing in vitro. A systematic comparison between the f-MWNT series in terms of cellular uptake, cytotoxicity, and siRNA complexation is offered. Significant improvement in siRNA delivery with the dendron-MWNT conjugates is shown, and gene silencing was obtained in 2 human cell lines using 2 different siRNA sequences. The study reveals that through f-MWNT structure-biological function analysis novel nanotube-based siRNA transfer vectors can be designed with minimal cytotoxicity and effective delivery and gene-silencing capabilities.
den Bossche Jeroen Van, Al-Jamal Wafa' T, Tian Bowen, Nunes Antonio, Fabbro Chiara, Bianco Alberto, Prato Maurizio, Kostarelos Kostas
Efficient receptor-independent intracellular translocation of aptamers mediated by conjugation to carbon nanotubes Article de journal
Dans: Chemical Communications (Cambridge, England), vol. 46, no. 39, p. 7379–7381, 2010, ISSN: 1364-548X.
Résumé | Liens | BibTeX | Étiquettes: Aptamers, Base Sequence, Biological Transport, carbon, Cell Line, Cell Surface, DNA Primers, Electron, Electrophoresis, Humans, I2CT, Microscopy, Nanotubes, Nucleotide, Polyacrylamide Gel, Receptors, Team-Bianco, Transmission, tumor
@article{van_den_bossche_efficient_2010,
title = {Efficient receptor-independent intracellular translocation of aptamers mediated by conjugation to carbon nanotubes},
author = {Jeroen Van den Bossche and Wafa' T Al-Jamal and Bowen Tian and Antonio Nunes and Chiara Fabbro and Alberto Bianco and Maurizio Prato and Kostas Kostarelos},
doi = {10.1039/c0cc02092c},
issn = {1364-548X},
year = {2010},
date = {2010-10-01},
journal = {Chemical Communications (Cambridge, England)},
volume = {46},
number = {39},
pages = {7379--7381},
abstract = {We have covalently grafted aptamers onto carboxylated carbon nanotubes to design a novel vector system that can easily translocate into the cytosol of different cell types independent of receptor-mediated uptake. We propose the use of carbon nanotubes for the efficient intracellular delivery of biologically active aptamers for potential therapeutic applications.},
keywords = {Aptamers, Base Sequence, Biological Transport, carbon, Cell Line, Cell Surface, DNA Primers, Electron, Electrophoresis, Humans, I2CT, Microscopy, Nanotubes, Nucleotide, Polyacrylamide Gel, Receptors, Team-Bianco, Transmission, tumor},
pubstate = {published},
tppubtype = {article}
}
We have covalently grafted aptamers onto carboxylated carbon nanotubes to design a novel vector system that can easily translocate into the cytosol of different cell types independent of receptor-mediated uptake. We propose the use of carbon nanotubes for the efficient intracellular delivery of biologically active aptamers for potential therapeutic applications.
Samorì Cristian, Sainz Raquel, Ménard-Moyon Cécilia, Toma Francesca M, Venturelli Enrica, Singh Prabhpreet, Ballestri Marco, Prato Maurizio, Bianco Alberto
Potentiometric titration as a straightforward method to assess the number of functional groups on shortened carbon nanotubes Article de journal
Dans: Carbon, vol. 48, no. 9, p. 2447–2454, 2010, ISSN: 0008-6223.
Résumé | Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{samori_potentiometric_2010,
title = {Potentiometric titration as a straightforward method to assess the number of functional groups on shortened carbon nanotubes},
author = {Cristian Samorì and Raquel Sainz and Cécilia Ménard-Moyon and Francesca M Toma and Enrica Venturelli and Prabhpreet Singh and Marco Ballestri and Maurizio Prato and Alberto Bianco},
url = {http://www.sciencedirect.com/science/article/pii/S0008622310001806},
doi = {10.1016/j.carbon.2010.03.015},
issn = {0008-6223},
year = {2010},
date = {2010-08-01},
urldate = {2020-03-31},
journal = {Carbon},
volume = {48},
number = {9},
pages = {2447--2454},
abstract = {The conditions for oxidizing multi-walled carbon nanotubes to shorten them to a narrow length distribution have been optimized. One of the most difficult achievements is to fully characterize this material from a chemical point of view, and to find a good quantitative correlation among different techniques. Herein, we report on the combination of different methods to determine the number of functional groups generated during strong acid treatment and a further amidation reaction. A good correlation was found using the colorimetric Kaiser test, thermogravimetric analysis and potentiometric argentometric titration. The final technique, used for the first time in this field, is highly versatile and, being non-destructive, allows a complete recovery of the starting material. Short carbon nanotubes are particularly useful for applications in biomedicine, and the control and precise assessment of their functionalization is critical when used as carriers for therapeutic molecules.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
The conditions for oxidizing multi-walled carbon nanotubes to shorten them to a narrow length distribution have been optimized. One of the most difficult achievements is to fully characterize this material from a chemical point of view, and to find a good quantitative correlation among different techniques. Herein, we report on the combination of different methods to determine the number of functional groups generated during strong acid treatment and a further amidation reaction. A good correlation was found using the colorimetric Kaiser test, thermogravimetric analysis and potentiometric argentometric titration. The final technique, used for the first time in this field, is highly versatile and, being non-destructive, allows a complete recovery of the starting material. Short carbon nanotubes are particularly useful for applications in biomedicine, and the control and precise assessment of their functionalization is critical when used as carriers for therapeutic molecules.
Fabre Bruno, Ababou-Girard Soraya, Singh Prabhpreet, Kumar Jitendra, Verma Sandeep, Bianco Alberto
Noncovalent assembly of ferrocene on modified gold surfaces mediated by uracil–adenine base pairs Article de journal
Dans: Electrochemistry Communications, vol. 12, no. 6, p. 831–834, 2010, ISSN: 1388-2481.
Résumé | Liens | BibTeX | Étiquettes: Electrochemistry,