Publications
2002
Schuster C, Isel C, Imbert I, Ehresmann C, Marquet R, Kieny M P
Secondary structure of the 3' terminus of hepatitis C virus minus-strand RNA Article de journal
Dans: J Virol, vol. 76, no. 16, p. 8058-8068, 2002, ISBN: 12134011, (0022-538x Journal Article).
Résumé | Liens | BibTeX | Étiquettes: Base Sequence DNA, MARQUET, Molecular Molecular Probe Techniques Molecular Sequence Data Nucleic Acid Conformation Plasmids/genetics RNA, Non-U.S. Gov't Virus Replication, Unité ARN, Viral/*chemistry/genetics Support, Viral/genetics Hepacivirus/*chemistry/genetics/physiology Human Models
@article{,
title = {Secondary structure of the 3' terminus of hepatitis C virus minus-strand RNA},
author = {C Schuster and C Isel and I Imbert and C Ehresmann and R Marquet and M P Kieny},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12134011},
isbn = {12134011},
year = {2002},
date = {2002-01-01},
journal = {J Virol},
volume = {76},
number = {16},
pages = {8058-8068},
abstract = {The 3'-terminal ends of both the positive and negative strands of the hepatitis C virus (HCV) RNA, the latter being the replicative intermediate, are most likely the initiation sites for replication by the viral RNA-dependent RNA polymerase, NS5B. The structural features of the very conserved 3' plus [(+)] strand untranslated region [3' (+) UTR] are well established (K. J. Blight and C. M. Rice, J. Virol. 71:7345-7352, 1997). However, little information is available concerning the 3' end of the minus [(-)] strand RNA. In the present work, we used chemical and enzymatic probing to investigate the conformation of that region, which is complementary to the 5' (+) UTR and the first 74 nucleotides of the HCV polyprotein coding sequence. By combining our experimental data with computer predictions, we have derived a secondary-structure model of this region. In our model, the last 220 nucleotides, where initiation of the (+) strand RNA synthesis presumably takes place, fold into five stable stem-loops, forming domain I. Domain I is linked to an overall less stable structure, named domain II, containing the sequences complementary to the pseudoknot of the internal ribosomal entry site in the 5' (+) UTR. Our results show that, even though the (-) strand 3'-terminal region has the antisense sequence of the 5' (+) UTR, it does not fold into its mirror image. Interestingly, comparison of the replication initiation sites on both strands reveals common structural features that may play key functions in the replication process.},
note = {0022-538x
Journal Article},
keywords = {Base Sequence DNA, MARQUET, Molecular Molecular Probe Techniques Molecular Sequence Data Nucleic Acid Conformation Plasmids/genetics RNA, Non-U.S. Gov't Virus Replication, Unité ARN, Viral/*chemistry/genetics Support, Viral/genetics Hepacivirus/*chemistry/genetics/physiology Human Models},
pubstate = {published},
tppubtype = {article}
}
The 3'-terminal ends of both the positive and negative strands of the hepatitis C virus (HCV) RNA, the latter being the replicative intermediate, are most likely the initiation sites for replication by the viral RNA-dependent RNA polymerase, NS5B. The structural features of the very conserved 3' plus [(+)] strand untranslated region [3' (+) UTR] are well established (K. J. Blight and C. M. Rice, J. Virol. 71:7345-7352, 1997). However, little information is available concerning the 3' end of the minus [(-)] strand RNA. In the present work, we used chemical and enzymatic probing to investigate the conformation of that region, which is complementary to the 5' (+) UTR and the first 74 nucleotides of the HCV polyprotein coding sequence. By combining our experimental data with computer predictions, we have derived a secondary-structure model of this region. In our model, the last 220 nucleotides, where initiation of the (+) strand RNA synthesis presumably takes place, fold into five stable stem-loops, forming domain I. Domain I is linked to an overall less stable structure, named domain II, containing the sequences complementary to the pseudoknot of the internal ribosomal entry site in the 5' (+) UTR. Our results show that, even though the (-) strand 3'-terminal region has the antisense sequence of the 5' (+) UTR, it does not fold into its mirror image. Interestingly, comparison of the replication initiation sites on both strands reveals common structural features that may play key functions in the replication process.