Imagerie PI2

@article{,

title = {AKAP149 binds to HIV-1 reverse transcriptase and is involved in the reverse transcription},

author = {J Lemay and P Maidou-Peindara and R Cancio and E Ennifar and G Coadou and G Maga and J C Rain and R Benarous and L X Liu},

url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18786546},

isbn = {18786546},

year  = {2008},

date = {2008-01-01},

journal = {J Mol Biol},

volume = {383},

number = {4},

pages = {783-796},

abstract = {Like all retroviruses, human immunodeficiency virus type 1 (HIV-1) undergoes reverse transcription during its replication cycle. The cellular cofactors potentially involved in this process still remain to be identified. We show here that A-kinase anchoring protein 149 (AKAP149) interacts with HIV-1 reverse transcriptase (RT) in both the yeast two-hybrid system and human cells. The AKAP149 binding site has been mapped to the RNase H domain of HIV-1 RT. AKAP149 silencing by RNA interference in HIV-1-infected cells inhibited viral replication at the reverse transcription step. We selected single-point mutants of RT defective for AKAP149 binding and demonstrated that mutant G462R, despite retaining significant intrinsic RT activity in vitro, failed to carry out HIV-1 reverse transcription correctly in infected cells. This suggests that the interaction between RT and AKAP149 in infected cells may play an important role in HIV-1 reverse transcription.},

note = {1089-8638 (Electronic) 

Journal Article 

Research Support, Non-U.S. Gov't},

keywords = {DUMAS  A Kinase Anchor Proteins/chemistry/genetics/*metabolism  Amino Acid Sequence  Animals  Binding Sites  Cell Line  HIV Reverse Transcriptase/chemistry/genetics/*metabolism  HIV-1/enzymology/genetics  Humans  Models, ENNIFAR, Molecular  Molecular Sequence Data  Mutagenesis  Protein Binding  Protein Structure, Tertiary  RNA Interference  Recombinant Fusion Proteins/genetics/metabolism  Reverse Transcription/*physiology  Two-Hybrid System Techniques  Virion/metabolism  Virus Replication/physiology, Unité ARN},

pubstate = {published},

tppubtype = {article}

}