Publications
2000
Fagegaltier D, Lescure A, Walczak R, Carbon P, Krol A
Structural analysis of new local features in SECIS RNA hairpins Article de journal
Dans: Nucleic Acids Res, vol. 28, no. 14, p. 2679-2689, 2000, ISBN: 10908323, (1362-4962 Journal Article).
Résumé | Liens | BibTeX | Étiquettes: Animals Base Sequence COS Cells DNA/chemistry/genetics DNA, DNA Support, Factual Drosophila melanogaster/genetics Glutathione Peroxidase/genetics/metabolism Human Mice Molecular Sequence Data Mutagenesis, LESCURE, Non-U.S. Gov't Xenopus laevis, Nucleic Acid/*genetics Selenocysteine/*genetics/metabolism Sequence Alignment Sequence Analysis, Recombinant/genetics/metabolism Databases, Site-Directed Nucleic Acid Conformation Phosphotransferases/genetics RNA/chemistry/*genetics Rats Regulatory Sequences, Unité ARN
@article{,
title = {Structural analysis of new local features in SECIS RNA hairpins},
author = {D Fagegaltier and A Lescure and R Walczak and P Carbon and A Krol},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10908323},
isbn = {10908323},
year = {2000},
date = {2000-01-01},
journal = {Nucleic Acids Res},
volume = {28},
number = {14},
pages = {2679-2689},
abstract = {Decoding of the UGA selenocysteine codon for selenoprotein translation requires the SECIS element, a stem-loop motif in the 3'-UTR of the mRNA carrying short or large apical loops. In previous structural studies, we derived a secondary structure model for SECIS RNAs with short apical loops. Work from others proposed that intra-apical loop base pairing can occur in those SECIS that possess large apical loops, yielding form 2 SECIS versus the form 1 with short loops. In this work, SECIS elements arising from eight different selenoprotein mRNAs were assayed by enzymatic and/or chemical probing showing that seven can adopt form 2. Further, database searches led to the discovery in drosophila and zebrafish of SECIS elements in the selenophosphate synthetase 2, type 1 deiodinase and SelW mRNAs. Alignment of SECIS sequences not only highlighted the predominance of form 2 but also made it possible to classify the SECIS elements according to the type of selenoprotein mRNA they belong to. Interestingly, the alignment revealed that an unpaired adenine, previously thought to be invariant, is replaced by a guanine in four SECIS elements. Tested in vivo, neither the A to G nor the A to U changes at this position greatly affected the activity while the most detrimental effect was provided by a C. The putative contribution of the various SECIS motifs to function and ligand binding is discussed.},
note = {1362-4962
Journal Article},
keywords = {Animals Base Sequence COS Cells DNA/chemistry/genetics DNA, DNA Support, Factual Drosophila melanogaster/genetics Glutathione Peroxidase/genetics/metabolism Human Mice Molecular Sequence Data Mutagenesis, LESCURE, Non-U.S. Gov't Xenopus laevis, Nucleic Acid/*genetics Selenocysteine/*genetics/metabolism Sequence Alignment Sequence Analysis, Recombinant/genetics/metabolism Databases, Site-Directed Nucleic Acid Conformation Phosphotransferases/genetics RNA/chemistry/*genetics Rats Regulatory Sequences, Unité ARN},
pubstate = {published},
tppubtype = {article}
}