Publications
1998
Braun A, Hoffmann Jules A, Meister Marie
Analysis of the Drosophila host defense in domino mutant larvae, which are devoid of hemocytes Article de journal
Dans: Proc. Natl. Acad. Sci. U.S.A., vol. 95, no. 24, p. 14337–14342, 1998, ISSN: 0027-8424.
Résumé | BibTeX | Étiquettes: Adipose Tissue, Animals, Candida, Escherichia coli, Fungal, Genotype, Hemocytes, hoffmann, Larva, M3i, Melanins, Micrococcus luteus, Spores
@article{braun_analysis_1998,
title = {Analysis of the Drosophila host defense in domino mutant larvae, which are devoid of hemocytes},
author = {A Braun and Jules A Hoffmann and Marie Meister},
issn = {0027-8424},
year = {1998},
date = {1998-11-01},
journal = {Proc. Natl. Acad. Sci. U.S.A.},
volume = {95},
number = {24},
pages = {14337--14342},
abstract = {We have analyzed the Drosophila immune response in domino mutant larvae, which are devoid of blood cells. The domino mutants have a good larval viability, but they die as prepupae. We show that, on immune challenge, induction of the genes encoding antimicrobial peptides in the fat body is not affected significantly in the mutant larvae, indicating that hemocytes are not essential in this process. The hemocoele of domino larvae contains numerous live microorganisms, the presence of which induces a weak antimicrobial response in the fat body. A full response is observed only after septic injury. We propose that the fat body cells are activated both by the presence of microorganisms and by injury and that injury potentiates the effect of microorganisms. Survival experiments after an immune challenge showed that domino mutants devoid of blood cells maintain a wild-type resistance to septic injury. This resistance was also observed in mutant larvae in which the synthesis of antibacterial peptides is impaired (immune deficiency larvae) and in mutants that are deficient for humoral melanization (Black cells larvae). However, if domino was combined with either the immune deficiency or the Black cell mutation, the resistance to septic injury was reduced severely. These results establish the relevance of the three immune reactions: phagocytosis, synthesis of antibacterial peptides, and melanization. By working in synergy, they provide Drosophila a highly effective defense against injury and/or infection.},
keywords = {Adipose Tissue, Animals, Candida, Escherichia coli, Fungal, Genotype, Hemocytes, hoffmann, Larva, M3i, Melanins, Micrococcus luteus, Spores},
pubstate = {published},
tppubtype = {article}
}
1992
Reichhart Jean-Marc, Meister Marie, Dimarcq Jean-Luc, Zachary Daniel, Hoffmann Danièle, Ruiz C, Richards G, Hoffmann Jules A
Insect immunity: developmental and inducible activity of the Drosophila diptericin promoter Article de journal
Dans: EMBO J., vol. 11, no. 4, p. 1469–1477, 1992, ISSN: 0261-4189.
Résumé | BibTeX | Étiquettes: Acute-Phase Proteins, Adipose Tissue, Animals, Base Sequence, beta-Galactosidase, Embryo, Gene Expression Regulation, Genetic, hoffmann, Insect Hormones, Insect Proteins, M3i, Mammals, Nonmammalian, Oligodeoxyribonucleotides, Promoter Regions, Recombinant Fusion Proteins, reichhart, Restriction Mapping
@article{reichhart_insect_1992,
title = {Insect immunity: developmental and inducible activity of the Drosophila diptericin promoter},
author = {Jean-Marc Reichhart and Marie Meister and Jean-Luc Dimarcq and Daniel Zachary and Danièle Hoffmann and C Ruiz and G Richards and Jules A Hoffmann},
issn = {0261-4189},
year = {1992},
date = {1992-01-01},
journal = {EMBO J.},
volume = {11},
number = {4},
pages = {1469--1477},
abstract = {Diptericins are 9 kDa inducible antibacterial peptides initially isolated from immune haemolymph of Phormia (Diptera). Following the isolation of a Drosophila cDNA encoding a diptericin homologue, we have now cloned a genomic fragment containing the Drosophila diptericin gene. To dissect the regulation of this gene, we have transformed flies with a fusion gene in which the reporter beta-galactosidase gene is under the control of 2.2 kb upstream sequences of the diptericin gene. We show that such a fusion gene is inducible by injection of live bacteria or complete Freund's adjuvant and respects the tissue specific expression pattern of the resident diptericin gene. Our analysis reveals at least four distinct phases in the regulation of this gene: young larvae, late third instar larvae, pupae and adults. This complexity may be related to the presence in the upstream sequences of multiple copies of response elements previously characterized in genes encoding acute phase response proteins in mammals (e.g. NK-kappa B, NF-kappa B related, NF-IL6 response elements).},
keywords = {Acute-Phase Proteins, Adipose Tissue, Animals, Base Sequence, beta-Galactosidase, Embryo, Gene Expression Regulation, Genetic, hoffmann, Insect Hormones, Insect Proteins, M3i, Mammals, Nonmammalian, Oligodeoxyribonucleotides, Promoter Regions, Recombinant Fusion Proteins, reichhart, Restriction Mapping},
pubstate = {published},
tppubtype = {article}
}
1976
Lagueux Marie, Hirn M, de Reggi M, Hoffmann Jules A
Ecdysteroid levels and ovarian development in adult female Locust migratoria Article de journal
Dans: C.R. Hebd. Seances Acad. Sci., Ser. D, Sci. Nat., vol. 282, no. 12, p. 1187–1190, 1976.
Résumé | BibTeX | Étiquettes: Adipose Tissue, Animals, Castration, Female, Grasshoppers, Hemolymph, hoffmann, Invertebrate Hormones, M3i, Neurosecretory Systems, Oogenesis, Ovary, Oviposition
@article{lagueux_ecdysteroid_1976,
title = {Ecdysteroid levels and ovarian development in adult female Locust migratoria},
author = {Marie Lagueux and M Hirn and M de Reggi and Jules A Hoffmann},
year = {1976},
date = {1976-03-01},
journal = {C.R. Hebd. Seances Acad. Sci., Ser. D, Sci. Nat.},
volume = {282},
number = {12},
pages = {1187--1190},
abstract = {During ovarian development in adult females of Locusta, an important biosynthesis of ecdysteroids is observed at the end of the maturation process of the terminal oocyte. This phenomenon occurs regularly during the different ovarian cycles. No synthesis is observed in allatectomized or ovariectomized female adults.},
keywords = {Adipose Tissue, Animals, Castration, Female, Grasshoppers, Hemolymph, hoffmann, Invertebrate Hormones, M3i, Neurosecretory Systems, Oogenesis, Ovary, Oviposition},
pubstate = {published},
tppubtype = {article}
}