Publications
2004
Mohr S., Bottin M. C., Lannes B., Neuville A., Bellocq J. P., Keith G., Rihn B. H.
Microdissection, mRNA amplification and microarray: a study of pleural mesothelial and malignant mesothelioma cells Article de journal
Dans: Biochimie, vol. 86, no. 1, p. 13-9, 2004, (0300-9084 Journal Article).
Résumé | BibTeX | Étiquettes: Analysis, Array, Chain, Epithelium/*metabolism, Expression, Female, Gene, Genetic, Human, KEITH, Lasers, Male, Markers, Mesothelioma/*genetics/metabolism, messenger, Microdissection, Neoplasms/*genetics/metabolism, Neoplastic/*genetics, Oligonucleotide, Pleura/*cytology/*metabolism, Pleural, Polymerase, Profiling, Reaction, Regulation, Reverse, RNA, Sequence, Transcriptase
@article{,
title = {Microdissection, mRNA amplification and microarray: a study of pleural mesothelial and malignant mesothelioma cells},
author = { S. Mohr and M.C. Bottin and B. Lannes and A. Neuville and J.P. Bellocq and G. Keith and B.H. Rihn},
year = {2004},
date = {2004-01-01},
journal = {Biochimie},
volume = {86},
number = {1},
pages = {13-9},
abstract = {The studies of molecular alterations in tumor cells with microarrays are often hampered by inherent tissue heterogeneity. The emergence of Laser Capture Microdissection (LCM) allowed us to overcome this challenge since it gives selective access to cancer cells that are isolated from their native tissue environment. In this report, we microdissected mesothelial cells and malignant mesothelioma cells of ex vivo resected specimens using LCM. Amplified RNA from mesothelial and mesothelioma microdissected cells allowed us to measure global gene expression with 10 K-microarrays in four independent experiments. We screened 9850 annotated human genes, 1275 of which have satisfied our data analysis requirements. They included 302 overexpressed genes and 160 downregulated genes in mesothelioma microdissected cells as compared to mesothelial microdissected cells. Among them, the expression levels of eight genes, namely BF, FTL, IGFBP7, RARRES1, RARRES2, RBP1, SAT, and TXN according to HUGO nomenclature, were increased, whereas six: ALOX5AP, CLNS1A, EIF4A2, ELK3, REQ and SYPL, were found to be underexpressed in mesothelioma microdissected cells. The ferritin light polypeptide (FTL) gene overexpression was confirmed by real time quantitative PCR. Our approach allowed a comprehensive in situ examination of mesothelioma and provided an accurate way to find new marker genes that may be useful for diagnosis and treatment of malignant pleural mesothelioma.},
note = {0300-9084
Journal Article},
keywords = {Analysis, Array, Chain, Epithelium/*metabolism, Expression, Female, Gene, Genetic, Human, KEITH, Lasers, Male, Markers, Mesothelioma/*genetics/metabolism, messenger, Microdissection, Neoplasms/*genetics/metabolism, Neoplastic/*genetics, Oligonucleotide, Pleura/*cytology/*metabolism, Pleural, Polymerase, Profiling, Reaction, Regulation, Reverse, RNA, Sequence, Transcriptase},
pubstate = {published},
tppubtype = {article}
}
Mohr S., Keith G., Galateau-Salle F., Icard P., Rihn B. H.
Cell protection, resistance and invasiveness of two malignant mesotheliomas as assessed by 10K-microarray Article de journal
Dans: Biochim Biophys Acta-Mol Basis Dis, vol. 1688, no. 1, p. 43-60, 2004, (0006-3002 Journal Article).
Résumé | BibTeX | Étiquettes: Analysis, Array, Biological/genetics, Cell, Expression, Family, Gene, Human, KEITH, Line, Markers, Mesothelioma/etiology/genetics/*pathology, Messenger/analysis, Multigene, Neoplasms/etiology/genetics/*pathology, Neoplastic, Pleural, Profiling, Protein, Regulation, RNA, tumor
@article{,
title = {Cell protection, resistance and invasiveness of two malignant mesotheliomas as assessed by 10K-microarray},
author = { S. Mohr and G. Keith and F. Galateau-Salle and P. Icard and B. H. Rihn},
year = {2004},
date = {2004-01-01},
journal = {Biochim Biophys Acta-Mol Basis Dis},
volume = {1688},
number = {1},
pages = {43-60},
abstract = {Malignant pleural mesothelioma (MPM) is an aggressive serosal tumor, strongly associated with former exposure to asbestos fibers and for which there is currently no effective treatment available. In human, MPM is characterized by a high local invasiveness, poor prognosis and therapeutic outcomes. In order to assess molecular changes that specify this phenotype, we performed a global gene expression profiling of human MPM. Using a 10,000-element microarray, we analyzed mRNA relative gene expression levels by comparing a mesothelioma cell line to either a pleural cell line or tumor specimens. To analyze these gene expression data, we used various bioinformatics softwares. Hierarchical clustering methods were used to group genes and samples with similar expression in an unsupervised mode. Genes of known function were further sorted by enzyme, function and pathway clusters using a supervised software (IncyteGenomics). Taken together, these data defined a molecular fingerprint of human MPM with more than 700 up- or down-regulated genes related to several traits of the malignant phenotype, specially associated with MPM invasiveness, protection and resistance to anticancer defenses. This portrait is meaningful in disease classification and management, and relevant in finding new specific markers of MPM. These molecular markers should improve the accuracy of mesothelioma diagnosis, prognosis and therapy.},
note = {0006-3002
Journal Article},
keywords = {Analysis, Array, Biological/genetics, Cell, Expression, Family, Gene, Human, KEITH, Line, Markers, Mesothelioma/etiology/genetics/*pathology, Messenger/analysis, Multigene, Neoplasms/etiology/genetics/*pathology, Neoplastic, Pleural, Profiling, Protein, Regulation, RNA, tumor},
pubstate = {published},
tppubtype = {article}
}
1994
Santos M. A., el-Adlouni C., Cox A. D., Luz J. M., Keith G., Tuite M. F.
Transfer RNA profiling: a new method for the identification of pathogenic Candida species Article de journal
Dans: Yeast, vol. 10, no. 5, p. 625-36, 1994, (0749-503x Journal Article).
Résumé | BibTeX | Étiquettes: Candida/classification/*genetics/pathogenicity, Electrophoresis, Fungal, Gel, Genetic, Gov't, Markers, Non-U.S., Polyacrylamide, RNA, Support, Transfer/*analysis
@article{,
title = {Transfer RNA profiling: a new method for the identification of pathogenic Candida species},
author = { M. A. Santos and C. el-Adlouni and A. D. Cox and J. M. Luz and G. Keith and M. F. Tuite},
year = {1994},
date = {1994-01-01},
journal = {Yeast},
volume = {10},
number = {5},
pages = {625-36},
abstract = {A new molecular taxonomic method applicable to the identification of medically important Candida species and other yeast species has been developed. It is based on the electrophoretic pattern of total tRNA samples (a 'tRNA profile') isolated from Candida species and generated using high-resolution semi-denaturing urea-polyacrylamide gel electrophoresis and methylene blue staining. Species-specific tRNA profiles for the species C. albicans, C. tropicalis, C. parapsilosis, C. guilliermondii, C. glabrata and Pichia guilliermondii were obtained. Detailed studies with the major human pathogen of the Candida genus, C. albicans, demonstrated that the tRNA profile for a given species was both reproducible and strain-independent; seven different C. albicans strains generated identical tRNA profiles. Minor strain-specific heterogeneities in the tRNA profiles of C. guilliermondii and C. parapsilosis were detected, but in neither case did they significantly alter the species-specific diagnostic tRNA profile. The potential of this method in clarifying taxonomic anomalies was demonstrated by the finding that Type I and Type II strains of C. stellatoidea generate very different tRNA profiles, with that of a Type II strain being identical to the C. albicans tRNA profile. This method offers a number of advantages over current electrophoretic karyotype methods for species identification, both within the Candida genus and with yeast species in general.},
note = {0749-503x
Journal Article},
keywords = {Candida/classification/*genetics/pathogenicity, Electrophoresis, Fungal, Gel, Genetic, Gov't, Markers, Non-U.S., Polyacrylamide, RNA, Support, Transfer/*analysis},
pubstate = {published},
tppubtype = {article}
}