Imagerie PI2

@article{mueller_polymerase_1997,

title = {Polymerase chain reaction-based identification of a novel serpin from human dendritic cells},

author = {C G F Mueller and S Ho and C Massacrier and S Lebecque and Y J Liu},

year  = {1997},

date = {1997-01-01},

journal = {European Journal of Immunology},

volume = {27},

pages = {3130--3134},

keywords = {Dendritic Cells, Human, Serpin},

pubstate = {published},

tppubtype = {article}

}

@article{,

title = {2'-O-methyl-5-formylcytidine (f5Cm), a new modified nucleotide at the 'wobble' of two cytoplasmic tRNAs Leu (NAA) from bovine liver},

author = { J. P. Pais de Barros and G. Keith and C. El Adlouni and A. L. Glasser and G. Mack and G. Dirheimer and J. Desgres},

year  = {1996},

date = {1996-01-01},

journal = {Nucleic Acids Res},

volume = {24},

number = {8},

pages = {1489-96},

abstract = {The nucleotide analysis of a cytoplasmic tRNA(Leu) isolated from bovine liver revealed the presence of an unknown modified nucleotide N. The corresponding N nucleoside was isolated by different enzymatic and chromatographic protocols from a partially purified preparation of this tRNA(Leu). Its chemical characterization was determined from its chromatographic properties, UV-absorption spectroscopy and mass spectrometric measurements, as well as from those of the borohydride reduced N nucleoside and its etheno-trimethylsilyl derivative. The structure of N was established as 2'-O-methyl-5-formylcytidine (f5CM), and its reduced derivative as 2'-O-methyl-5-hydroxy-methylcytidine (om5Cm). By sequencing the bovine liver tRNA(Leu), the structure of the anticodon was determined as f5CmAA. In addition, the nucleotide sequence showed two primary structures differing only by the nucleotide 47c which is either uridine or adenosine. The two slightly differing bovine liver tRNAs-Leu(f5CmAA) are the only tRNAs so far sequenced which contain f5Cm. The role of such a modified cytidine at the first position of the anticodon is discussed in terms of decoding properties for the UUG and UUA leucine codons. Recently, precise evidence was obtained for the presence of f5Cm at the same position in tRNAs(Leu)(NAA) isolated from rabbit and lamb liver. Therefore, the 2'-O-methyl-5-formyl modification of cytidine at position 34 could be a general feature of cytoplasmic tRNAs(Leu)(NAA) in mammals.},

note = {0305-1048 

Journal Article},

keywords = {&, Acid, Acyl/*chemistry/isolation, Amino, Animals, Base, Borohydrides/chemistry, Cattle, Cells, Conformation, Cytidine/*analogs, Cytoplasm, Data, derivatives/chemistry/isolation, Fragmentography, Gov't, Hela, Human, Liver/*chemistry, Mass, Molecular, Non-U.S., Nucleic, purification, RNA, Sequence, structure, Support, Transfer},

pubstate = {published},

tppubtype = {article}

}

@article{,

title = {Variations in tRNA modifications, particularly of their queuine content in higher eukaryotes. Its relation to malignancy grading},

author = { G. Dirheimer and W. Baranowski and G. Keith},

year  = {1995},

date = {1995-01-01},

journal = {Biochimie},

volume = {77},

number = {1-2},

pages = {99-103},

abstract = {Literature references dealing with the variations in the modification level of nucleosides in total eukaryotic tRNAs as a function of different physiological status and after drug administration as well as in sequenced cytoplasmic tRNAs between normal and tumor cells and in SV40-transformed cells are reviewed. In addition, special attention is given to guanine replacement of queuine in the first position of the anticodon of tRNAs. A correlation between the level of this undermodification in cancer tissues and the malignancy grading could be found in human ovarian tumors, confirming the results reported in several laboratories for lymphomas and lung cancer tissues. Indeed tRNAs from primary and metastatic human ovarian malignant tumors are Q deficient as compared to tRNAs from normal tissues or benign tumors: thus queuine deficiency increases with malignancy and grading of differentiation.},

note = {0300-9084 

Journal Article 

Review 

Review, Tutorial},

keywords = {&, Animals, Cell, derivatives/analysis, Female, Gov't, Guanine/*analogs, Human, Neoplasms/*genetics/pathology, Neoplastic/genetics, Non-U.S., Ovarian, post-transcriptional, Processing, Purines/analysis, Pyrimidines/analysis, RNA, Support, Transfer/*chemistry/metabolism, Transformation},

pubstate = {published},

tppubtype = {article}

}

@article{,

title = {Postlabeling: a sensitive method for studying DNA adducts and their role in carcinogenesis},

author = { G. Keith and G. Dirheimer},

year  = {1995},

date = {1995-01-01},

journal = {Curr Opin Biotechnol},

volume = {6},

number = {1},

pages = {3-11},

abstract = {The covalent binding of xenobiotics to DNA is an important trigger of the multistage process that leads to carcinogenesis. 32P-postlabeling represents a highly sensitive method for biomonitoring exposure to genotoxic agents and for cancer risk assessment; it is capable of detecting less than one DNA adduct per human genome. Recent improvements to the technique have shown that the resistance of adducted DNA to enzyme digestion may lead to an overestimation of the number of different adducts present in a sample.},

note = {0958-1669 

Journal Article 

Review 

Review, Academic},

keywords = {*Cell, *Genome, Adducts/*analysis, and, Animals, Base, Cell, Conditions/genetics/pathology, Data, Dilution, Division, DNA, Genetic, Gov't, Human, Models, Molecular, Mutagenesis, Neoplasms/*genetics/pathology, Neoplastic, Non-U.S., Phosphorus, Precancerous, Radioisotope, Radioisotopes, Sensitivity, Sequence, Specificity, Support, Technique, Transformation, Xenobiotics},

pubstate = {published},

tppubtype = {article}

}

@article{,

title = {Deficiency of queuine, a highly modified purine base, in transfer RNAs from primary and metastatic ovarian malignant tumors in women},

author = { W. Baranowski and G. Dirheimer and J. A. Jakowicki and G. Keith},

year  = {1994},

date = {1994-01-01},

journal = {Cancer Res},

volume = {54},

number = {16},

pages = {4468-71},

abstract = {The tRNAs from rapidly growing tissues, particularly from neoplasia, often exhibit queuine deficiency. In order to check whether different kinds of ovarian tumors display queuine deficiencies we have analyzed tRNA samples from 16 ovarian malignancies. The tRNAs from histologically normal myometrium (4 samples) and myoma (6 samples) were taken as healthy tissue and benign tumor references. Queuine deficiency was determined by an exchange assay using [8-3H]guanine and tRNA:guanine transglycosylase from Escherichia coli. The mean values of queuine deficiencies in tRNAs were: 10.95 +/- 2.21 (SD) pmol/A260 in gonadal and germ cell tumors (5 cases); 23.75 +/- 7.89 pmol/A260 in primary epithelial tumors (9 cases); and 34.58 +/- 7.18 pmol/A260 in metastatic tumors (2 cases). These values displayed statistically significant differences (P = 0.0003, Kruskal-Wallis test). The queuine deficiencies in tRNAs significantly increased when moving from well-differentiated through moderately differentiated to poorly differentiated tumors, with the highest values found in poorly differentiated metastatic tumors (P = 0.0002, Kruskal-Wallis test). Queuine deficiency determination in tRNAs is proposed as a factor for clinical outcome prognosis of ovarian malignancies.},

note = {0008-5472 

Journal Article},

keywords = {&, Adolescent, Adult, Aged, derivatives/analysis, Female, Gov't, Guanine/*analogs, Human, Middle, Neoplasm/*chemistry, Neoplasms/*chemistry/pathology, Non-U.S., Ovarian, RNA, Support, Transfer/*chemistry},

pubstate = {published},

tppubtype = {article}

}

@article{,

title = {[Methylation of DNA in tissue of ovarian malignant tumors in women]},

author = { W. Baranowski and J. Tomaszewski and G. Keith},

year  = {1993},

date = {1993-01-01},

journal = {Ginekol Pol},

volume = {64},

number = {4},

pages = {169-73},

abstract = {DNA methylation level from woman ovarian malignant tissues was investigated by 32P postlabeling of the single nucleotides, separation on TLC with followed autoradiography and Cerenkov counting. Slight differences in DNA methylation extent were found in malignant ovarian tumors as compared to normal myometrium and benign uterine tumor [myomas]. Lowest level of m5dC in relation to C and also in relation to total DNA was found in carcinoma embryonal tissue whereas maximal DNA methylation level was obtained in folliculoma tissue. This preliminary report needs further investigation of particularly genes methylation.},

note = {0017-0011 

Journal Article},

keywords = {Abstract, Adult, Aged, Carcinoma/genetics, Cell, DNA, English, Female, Human, Methylation, Middle, Neoplasm/*metabolism, Neoplasms/*genetics, Ovarian, Sertoli, Tumor/genetics},

pubstate = {published},

tppubtype = {article}

}

@article{,

title = {Unusual deficiency of the modified purine base queuine in transfer ribonucleic acid from the human placenta as tested by enzymatic assay},

author = { W. Baranowski and J. Tomaszewski and G. Keith},

year  = {1993},

date = {1993-01-01},

journal = {Am J Obstet Gynecol},

volume = {169},

number = {3},

pages = {581-2},

abstract = {Transfer ribonucleic acid from rapidly growing tissues, particularly from neoplasia, is partially deficient in queuine, a highly modified transfer ribonucleic acid constituent. By means of an enzymatic assay we also found a queuine deficiency (14%) in human placenta transfer ribonucleic acid despite its high concentrations in the amniotic fluid. Proposed cause and significance of the results are discussed.},

note = {0002-9378 

Journal Article},

keywords = {&, derivatives/chemistry, Female, Guanine/*analogs, Human, Placenta/*chemistry, Pregnancy, RNA, Transfer/*chemistry},

pubstate = {published},

tppubtype = {article}

}

@article{,

title = {The major RNA in prosomes of HeLa cells and duck erythroblasts is tRNA(Lys,3)},

author = { H. G. Nothwang and O. Coux and G. Keith and I. Silva-Pereira and K. Scherrer},

year  = {1992},

date = {1992-01-01},

journal = {Nucleic Acids Res},

volume = {20},

number = {8},

pages = {1959-65},

abstract = {Two-dimensional gel electrophoresis of HeLa cell prosomal RNAs, 3'-end labeled by RNA ligase, revealed one prominent spot. Determination of a partial sequence at the 3'-end indicated full homology to the 18 nucleotides at the 3'-end of tRNA(Lys,3) from rabbit, the bovine and the human species. An oligonucleotide complementary to the 3'-end of tRNA(Lys,3) hybridized on Northern blots with prosomal RNA from both HeLa cells and duck erythroblasts. In two-dimensional PAGE, the major pRNA of HeLa cells co-migrated with bovine tRNA(Lys,3). Reconstitution of the CCA 3'-end of RNA from both human and duck prosomes, by tRNA-nucleotidyl-transferase, confirmed the tRNA character of this type of RNA. Furthermore, it revealed at least one additional tRNA band about 85 nt long among the prosomal RNA from both species. Finally, confirming an original property of prosomal RNA, we show that in vitro synthesized tRNA(Lys,3) hybridizes stably to duck globin mRNA, and to poly(A)(+)- and poly(A)(-)-RNA from HeLa cells.},

note = {0305-1048 

Journal Article},

keywords = {Animals, Base, Blotting, Cells, Data, Ducks, effects, Electrophoresis, Erythroblasts, Gel, Gov't, Hela, Human, Lys/*analysis/metabolism, Molecular, Non-U.S., Northern, Nucleotidyltransferases/metabolism, Ribonucleoproteins/*chemistry/drug, RNA, Sequence, Support, Transfer, Two-Dimensional, Zinc/pharmacology},

pubstate = {published},

tppubtype = {article}

}

@article{,

title = {Rapid transfer of small RNAs from a polyacrylamide gel onto a nylon membrane using a gel dryer},

author = { M. L. Wilhelm and W. Baranowski and G. Keith and F. X. Wilhelm},

year  = {1992},

date = {1992-01-01},

journal = {Nucleic Acids Res},

volume = {20},

number = {15},

pages = {4106},

note = {0305-1048 

Journal Article},

keywords = {&, 5S/*isolation, Acrylic, Human, Nuclear/*isolation, Nylons, purification, Resins, Ribosomal, RNA, Small, Transfer/*isolation, Yeasts/genetics},

pubstate = {published},

tppubtype = {article}

}