Publications
2006
Singh Ravi, Pantarotto Davide, Lacerda Lara, Pastorin Giorgia, Klumpp Cédric, Prato Maurizio, Bianco Alberto, Kostarelos Kostas
Tissue biodistribution and blood clearance rates of intravenously administered carbon nanotube radiotracers Article de journal
Dans: Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 9, p. 3357–3362, 2006, ISSN: 0027-8424.
Résumé | Liens | BibTeX | Étiquettes: Animals, carbon, Electron, Female, Half-Life, I2CT, Inbred BALB C, Indium Radioisotopes, Injections, Intravenous, Mice, Microscopy, Molecular Structure, Nanotubes, Pentetic Acid, Team-Bianco, Tissue Distribution, Transmission
@article{singh_tissue_2006,
title = {Tissue biodistribution and blood clearance rates of intravenously administered carbon nanotube radiotracers},
author = {Ravi Singh and Davide Pantarotto and Lara Lacerda and Giorgia Pastorin and Cédric Klumpp and Maurizio Prato and Alberto Bianco and Kostas Kostarelos},
doi = {10.1073/pnas.0509009103},
issn = {0027-8424},
year = {2006},
date = {2006-02-01},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {103},
number = {9},
pages = {3357--3362},
abstract = {Carbon nanotubes (CNT) are intensively being developed for biomedical applications including drug and gene delivery. Although all possible clinical applications will require compatibility of CNT with the biological milieu, their in vivo capabilities and limitations have not yet been explored. In this work, water-soluble, single-walled CNT (SWNT) have been functionalized with the chelating molecule diethylentriaminepentaacetic (DTPA) and labeled with indium ((111)In) for imaging purposes. Intravenous (i.v.) administration of these functionalized SWNT (f-SWNT) followed by radioactivity tracing using gamma scintigraphy indicated that f-SWNT are not retained in any of the reticuloendothelial system organs (liver or spleen) and are rapidly cleared from systemic blood circulation through the renal excretion route. The observed rapid blood clearance and half-life (3 h) of f-SWNT has major implications for all potential clinical uses of CNT. Moreover, urine excretion studies using both f-SWNT and functionalized multiwalled CNT followed by electron microscopy analysis of urine samples revealed that both types of nanotubes were excreted as intact nanotubes. This work describes the pharmacokinetic parameters of i.v. administered functionalized CNT relevant for various therapeutic and diagnostic applications.},
keywords = {Animals, carbon, Electron, Female, Half-Life, I2CT, Inbred BALB C, Indium Radioisotopes, Injections, Intravenous, Mice, Microscopy, Molecular Structure, Nanotubes, Pentetic Acid, Team-Bianco, Tissue Distribution, Transmission},
pubstate = {published},
tppubtype = {article}
}
2005
Bianco Alberto, Kostarelos Kostas, Prato Maurizio
Applications of carbon nanotubes in drug delivery Article de journal
Dans: Current Opinion in Chemical Biology, vol. 9, no. 6, p. 674–679, 2005, ISSN: 1367-5931.
Résumé | Liens | BibTeX | Étiquettes: Biological, carbon, Drug Delivery Systems, Electron, HeLa Cells, Humans, I2CT, Microscopy, Models, Nanotubes, Nucleic Acids, Peptides, scanning, Team-Bianco
@article{bianco_applications_2005,
title = {Applications of carbon nanotubes in drug delivery},
author = {Alberto Bianco and Kostas Kostarelos and Maurizio Prato},
doi = {10.1016/j.cbpa.2005.10.005},
issn = {1367-5931},
year = {2005},
date = {2005-12-01},
journal = {Current Opinion in Chemical Biology},
volume = {9},
number = {6},
pages = {674--679},
abstract = {The development of new and efficient drug delivery systems is of fundamental importance to improve the pharmacological profiles of many classes of therapeutic molecules. Many different types of drug delivery systems are currently available. Within the family of nanomaterials, carbon nanotubes (CNT) have emerged as a new alternative and efficient tool for transporting and translocating therapeutic molecules. CNT can be functionalised with bioactive peptides, proteins, nucleic acids and drugs, and used to deliver their cargos to cells and organs. Because functionalised CNT display low toxicity and are not immunogenic, such systems hold great potential in the field of nanobiotechnology and nanomedicine.},
keywords = {Biological, carbon, Drug Delivery Systems, Electron, HeLa Cells, Humans, I2CT, Microscopy, Models, Nanotubes, Nucleic Acids, Peptides, scanning, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Wu Wei, Wieckowski Sébastien, Pastorin Giorgia, Benincasa Monica, Klumpp Cédric, Briand Jean-Paul, Gennaro Renato, Prato Maurizio, Bianco Alberto
Targeted delivery of amphotericin B to cells by using functionalized carbon nanotubes Article de journal
Dans: Angewandte Chemie (International Ed. in English), vol. 44, no. 39, p. 6358–6362, 2005, ISSN: 1433-7851.
Liens | BibTeX | Étiquettes: Amphotericin B, Antifungal Agents, carbon, Drug Carriers, Drug Delivery Systems, Fungi, Humans, I2CT, Jurkat Cells, Molecular Structure, Nanotubes, Particle Size, Solubility, Surface Properties, Team-Bianco
@article{wu_targeted_2005,
title = {Targeted delivery of amphotericin B to cells by using functionalized carbon nanotubes},
author = {Wei Wu and Sébastien Wieckowski and Giorgia Pastorin and Monica Benincasa and Cédric Klumpp and Jean-Paul Briand and Renato Gennaro and Maurizio Prato and Alberto Bianco},
doi = {10.1002/anie.200501613},
issn = {1433-7851},
year = {2005},
date = {2005-10-01},
journal = {Angewandte Chemie (International Ed. in English)},
volume = {44},
number = {39},
pages = {6358--6362},
keywords = {Amphotericin B, Antifungal Agents, carbon, Drug Carriers, Drug Delivery Systems, Fungi, Humans, I2CT, Jurkat Cells, Molecular Structure, Nanotubes, Particle Size, Solubility, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Singh Ravi, Pantarotto Davide, McCarthy David, Chaloin Olivier, Hoebeke Johan, Partidos Charalambos D, Briand Jean-Paul, Prato Maurizio, Bianco Alberto, Kostarelos Kostas
Binding and condensation of plasmid DNA onto functionalized carbon nanotubes: toward the construction of nanotube-based gene delivery vectors Article de journal
Dans: Journal of the American Chemical Society, vol. 127, no. 12, p. 4388–4396, 2005, ISSN: 0002-7863.
Résumé | Liens | BibTeX | Étiquettes: carbon, Cations, DNA, Electron, Gene Transfer Techniques, Genetic Vectors, I2CT, Lysine, Microscopy, Nanotubes, Plasmids, Quaternary Ammonium Compounds, scanning, Surface Plasmon Resonance, Team-Bianco
@article{singh_binding_2005,
title = {Binding and condensation of plasmid DNA onto functionalized carbon nanotubes: toward the construction of nanotube-based gene delivery vectors},
author = {Ravi Singh and Davide Pantarotto and David McCarthy and Olivier Chaloin and Johan Hoebeke and Charalambos D Partidos and Jean-Paul Briand and Maurizio Prato and Alberto Bianco and Kostas Kostarelos},
doi = {10.1021/ja0441561},
issn = {0002-7863},
year = {2005},
date = {2005-03-01},
journal = {Journal of the American Chemical Society},
volume = {127},
number = {12},
pages = {4388--4396},
abstract = {Carbon nanotubes (CNTs) constitute a class of nanomaterials that possess characteristics suitable for a variety of possible applications. Their compatibility with aqueous environments has been made possible by the chemical functionalization of their surface, allowing for exploration of their interactions with biological components including mammalian cells. Functionalized CNTs (f-CNTs) are being intensively explored in advanced biotechnological applications ranging from molecular biosensors to cellular growth substrates. We have been exploring the potential of f-CNTs as delivery vehicles of biologically active molecules in view of possible biomedical applications, including vaccination and gene delivery. Recently we reported the capability of ammonium-functionalized single-walled CNTs to penetrate human and murine cells and facilitate the delivery of plasmid DNA leading to expression of marker genes. To optimize f-CNTs as gene delivery vehicles, it is essential to characterize their interactions with DNA. In the present report, we study the interactions of three types of f-CNTs, ammonium-functionalized single-walled and multiwalled carbon nanotubes (SWNT-NH3+; MWNT-NH3+), and lysine-functionalized single-walled carbon nanotubes (SWNT-Lys-NH3+), with plasmid DNA. Nanotube-DNA complexes were analyzed by scanning electron microscopy, surface plasmon resonance, PicoGreen dye exclusion, and agarose gel shift assay. The results indicate that all three types of cationic carbon nanotubes are able to condense DNA to varying degrees, indicating that both nanotube surface area and charge density are critical parameters that determine the interaction and electrostatic complex formation between f-CNTs with DNA. All three different f-CNT types in this study exhibited upregulation of marker gene expression over naked DNA using a mammalian (human) cell line. Differences in the levels of gene expression were correlated with the structural and biophysical data obtained for the f-CNT:DNA complexes to suggest that large surface area leading to very efficient DNA condensation is not necessary for effective gene transfer. However, it will require further investigation to determine whether the degree of binding and tight association between DNA and nanotubes is a desirable trait to increase gene expression efficiency in vitro or in vivo. This study constitutes the first thorough investigation into the physicochemical interactions between cationic functionalized carbon nanotubes and DNA toward construction of carbon nanotube-based gene transfer vector systems.},
keywords = {carbon, Cations, DNA, Electron, Gene Transfer Techniques, Genetic Vectors, I2CT, Lysine, Microscopy, Nanotubes, Plasmids, Quaternary Ammonium Compounds, scanning, Surface Plasmon Resonance, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Bianco Alberto, Kostarelos Kostas, Partidos Charalambos D, Prato Maurizio
Biomedical applications of functionalised carbon nanotubes Article de journal
Dans: Chemical Communications (Cambridge, England), no. 5, p. 571–577, 2005, ISSN: 1359-7345.
Résumé | Liens | BibTeX | Étiquettes: Antigens, carbon, Chemical, Drug Delivery Systems, Gene Transfer Techniques, Humans, I2CT, Models, Molecular Structure, nanotechnology, Nanotubes, Team-Bianco, Vaccines
@article{bianco_biomedical_2005,
title = {Biomedical applications of functionalised carbon nanotubes},
author = {Alberto Bianco and Kostas Kostarelos and Charalambos D Partidos and Maurizio Prato},
doi = {10.1039/b410943k},
issn = {1359-7345},
year = {2005},
date = {2005-01-01},
journal = {Chemical Communications (Cambridge, England)},
number = {5},
pages = {571--577},
abstract = {The organic functionalisation of carbon nanotubes can improve substantially their solubility and biocompatibility profile; as a consequence, their manipulation and integration into biological systems has become possible so that functionalised carbon nanotubes hold currently strong promise as novel systems for the delivery of drugs, antigens and genes.},
keywords = {Antigens, carbon, Chemical, Drug Delivery Systems, Gene Transfer Techniques, Humans, I2CT, Models, Molecular Structure, nanotechnology, Nanotubes, Team-Bianco, Vaccines},
pubstate = {published},
tppubtype = {article}
}
Bianco Alberto, Hoebeke Johan, Godefroy Sylvie, Chaloin Olivier, Pantarotto Davide, Briand Jean-Paul, Muller Sylviane, Prato Maurizio, Partidos Charalambos D
Cationic carbon nanotubes bind to CpG oligodeoxynucleotides and enhance their immunostimulatory properties Article de journal
Dans: Journal of the American Chemical Society, vol. 127, no. 1, p. 58–59, 2005, ISSN: 0002-7863.
Résumé | Liens | BibTeX | Étiquettes: Adjuvants, Animals, carbon, Cations, CpG Islands, I2CT, Immunologic, Interferon-gamma, Interleukin-6, Kinetics, Lymphocytes, Mice, Nanotubes, oligonucleotides, Surface Plasmon Resonance, Team-Bianco
@article{bianco_cationic_2005,
title = {Cationic carbon nanotubes bind to CpG oligodeoxynucleotides and enhance their immunostimulatory properties},
author = {Alberto Bianco and Johan Hoebeke and Sylvie Godefroy and Olivier Chaloin and Davide Pantarotto and Jean-Paul Briand and Sylviane Muller and Maurizio Prato and Charalambos D Partidos},
doi = {10.1021/ja044293y},
issn = {0002-7863},
year = {2005},
date = {2005-01-01},
journal = {Journal of the American Chemical Society},
volume = {127},
number = {1},
pages = {58--59},
abstract = {Functionalized cationic carbon nanotubes are able to form a stable complex with CpG ODN based on charge interaction and to increase the immunostimulatory activity of CpG motifs.},
keywords = {Adjuvants, Animals, carbon, Cations, CpG Islands, I2CT, Immunologic, Interferon-gamma, Interleukin-6, Kinetics, Lymphocytes, Mice, Nanotubes, oligonucleotides, Surface Plasmon Resonance, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
2004
Bianco Alberto
Carbon nanotubes for the delivery of therapeutic molecules Article de journal
Dans: Expert Opinion on Drug Delivery, vol. 1, no. 1, p. 57–65, 2004, ISSN: 1742-5247.
Résumé | Liens | BibTeX | Étiquettes: carbon, Drug Carriers, Gene Transfer Techniques, I2CT, Nanotubes, Pharmaceutical Preparations, Team-Bianco
@article{bianco_carbon_2004,
title = {Carbon nanotubes for the delivery of therapeutic molecules},
author = {Alberto Bianco},
doi = {10.1517/17425247.1.1.57},
issn = {1742-5247},
year = {2004},
date = {2004-11-01},
journal = {Expert Opinion on Drug Delivery},
volume = {1},
number = {1},
pages = {57--65},
abstract = {Functionalised carbon nanotubes (f-CNTs) are emerging as new tools in the field of nanobiotechnology and nanomedicine. This is because they can be easily manipulated and modified by encapsulation with biopolymers or by covalent linking of solubilising groups to the external walls and tips. The possibility of incorporating f-CNTs into biological systems has opened the way to the exploration of their potential applications in biology and medicinal chemistry. Within the different fields of applications (i.e., biosensors, composite materials, molecular electronics), one use of CNTs is as new carrier systems for the delivery of therapeutic molecules. Research discussed in this review is focused on recent advances in the development of CNT technology for the delivery of drugs, antigens and genes.},
keywords = {carbon, Drug Carriers, Gene Transfer Techniques, I2CT, Nanotubes, Pharmaceutical Preparations, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Pantarotto Davide, Briand Jean-Paul, Prato Maurizio, Bianco Alberto
Translocation of bioactive peptides across cell membranes by carbon nanotubes Article de journal
Dans: Chemical Communications (Cambridge, England), no. 1, p. 16–17, 2004, ISSN: 1359-7345.
Résumé | Liens | BibTeX | Étiquettes: 3T3 Cells, Animals, carbon, Cell Membrane, Confocal, Flow Cytometry, fluorescence, I2CT, Mice, Microscopy, Nanotubes, Particle Size, Peptides, Protein Transport, Team-Bianco
@article{pantarotto_translocation_2004,
title = {Translocation of bioactive peptides across cell membranes by carbon nanotubes},
author = {Davide Pantarotto and Jean-Paul Briand and Maurizio Prato and Alberto Bianco},
doi = {10.1039/b311254c},
issn = {1359-7345},
year = {2004},
date = {2004-01-01},
journal = {Chemical Communications (Cambridge, England)},
number = {1},
pages = {16--17},
abstract = {Functionalised carbon nanotubes are able to cross the cell membrane and to accumulate in the cytoplasm or reach the nucleus without being toxic for the cell up to 10 [micro sign]M.},
keywords = {3T3 Cells, Animals, carbon, Cell Membrane, Confocal, Flow Cytometry, fluorescence, I2CT, Mice, Microscopy, Nanotubes, Particle Size, Peptides, Protein Transport, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
2003
Pantarotto Davide, Partidos Charalambos D, Hoebeke Johan, Brown Fred, Kramer Ed, Briand Jean-Paul, Muller Sylviane, Prato Maurizio, Bianco Alberto
Immunization with peptide-functionalized carbon nanotubes enhances virus-specific neutralizing antibody responses Article de journal
Dans: Chemistry & Biology, vol. 10, no. 10, p. 961–966, 2003, ISSN: 1074-5521.
Liens | BibTeX | Étiquettes: Animals, Antibodies, Antigen-Antibody Reactions, carbon, Drug Delivery Systems, Epitopes, Foot-and-Mouth Disease Virus, I2CT, Immunization, Mice, Monoclonal, Nanotubes, Neutralization Tests, Peptides, Team-Bianco, Vaccines, Viral
@article{pantarotto_immunization_2003,
title = {Immunization with peptide-functionalized carbon nanotubes enhances virus-specific neutralizing antibody responses},
author = {Davide Pantarotto and Charalambos D Partidos and Johan Hoebeke and Fred Brown and Ed Kramer and Jean-Paul Briand and Sylviane Muller and Maurizio Prato and Alberto Bianco},
doi = {10.1016/j.chembiol.2003.09.011},
issn = {1074-5521},
year = {2003},
date = {2003-10-01},
journal = {Chemistry & Biology},
volume = {10},
number = {10},
pages = {961--966},
keywords = {Animals, Antibodies, Antigen-Antibody Reactions, carbon, Drug Delivery Systems, Epitopes, Foot-and-Mouth Disease Virus, I2CT, Immunization, Mice, Monoclonal, Nanotubes, Neutralization Tests, Peptides, Team-Bianco, Vaccines, Viral},
pubstate = {published},
tppubtype = {article}
}
Pantarotto Davide, Partidos Charalambos D, Graff Roland, Hoebeke Johan, Briand Jean-Paul, Prato Maurizio, Bianco Alberto
Synthesis, structural characterization, and immunological properties of carbon nanotubes functionalized with peptides Article de journal
Dans: Journal of the American Chemical Society, vol. 125, no. 20, p. 6160–6164, 2003, ISSN: 0002-7863.
Résumé | Liens | BibTeX | Étiquettes: B-Lymphocyte, biomolecular, Capsid Proteins, carbon, Chromatography, Epitopes, Foot-and-Mouth Disease Virus, High Pressure Liquid, I2CT, nanotechnology, Nanotubes, Nuclear Magnetic Resonance, Peptide Fragments, Team-Bianco
@article{pantarotto_synthesis_2003,
title = {Synthesis, structural characterization, and immunological properties of carbon nanotubes functionalized with peptides},
author = {Davide Pantarotto and Charalambos D Partidos and Roland Graff and Johan Hoebeke and Jean-Paul Briand and Maurizio Prato and Alberto Bianco},
doi = {10.1021/ja034342r},
issn = {0002-7863},
year = {2003},
date = {2003-05-01},
journal = {Journal of the American Chemical Society},
volume = {125},
number = {20},
pages = {6160--6164},
abstract = {Carbon nanotubes (NTs) are becoming highly attractive molecules for applications in medicinal chemistry. The main problem of insolubility in aqueous media has been solved by developing a synthetic protocol that allows highly water-soluble carbon NTs to be obtained. As a result, biologically active peptides can be easily linked through a stable covalent bond to carbon NTs. We have demonstrated that a bound peptide from the foot-and-mouth disease virus, corresponding to the 141-159 region of the viral envelope protein VP1, retained the structural integrity and was recognized by monoclonal and polyclonal antibodies. In addition, this peptide-NT conjugate is immunogenic, eliciting antibody responses of the right specificity. Such a system could be greatly advantageous for diagnostic purposes and could find future applications in vaccine delivery.},
keywords = {B-Lymphocyte, biomolecular, Capsid Proteins, carbon, Chromatography, Epitopes, Foot-and-Mouth Disease Virus, High Pressure Liquid, I2CT, nanotechnology, Nanotubes, Nuclear Magnetic Resonance, Peptide Fragments, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
2002
Georgakilas Vasilios, Tagmatarchis Nikos, Pantarotto Davide, Bianco Alberto, Briand Jean-Paul, Prato Maurizio
Amino acid functionalisation of water soluble carbon nanotubes Article de journal
Dans: Chemical Communications (Cambridge, England), no. 24, p. 3050–3051, 2002, ISSN: 1359-7345.
Résumé | Liens | BibTeX | Étiquettes: Amino Acids, carbon, I2CT, Nanotubes, Solubility, Team-Bianco, water
@article{georgakilas_amino_2002,
title = {Amino acid functionalisation of water soluble carbon nanotubes},
author = {Vasilios Georgakilas and Nikos Tagmatarchis and Davide Pantarotto and Alberto Bianco and Jean-Paul Briand and Maurizio Prato},
doi = {10.1039/b209843a},
issn = {1359-7345},
year = {2002},
date = {2002-12-01},
journal = {Chemical Communications (Cambridge, England)},
number = {24},
pages = {3050--3051},
abstract = {High solubility of SWNTs and MWNTs in water is obtained by organic functionalisation; derivatisation with N-protected glycine is also easily achieved.},
keywords = {Amino Acids, carbon, I2CT, Nanotubes, Solubility, Team-Bianco, water},
pubstate = {published},
tppubtype = {article}
}