Publications
2018
Gros Frédéric, Fournel Sylvie, Liégeois Samuel, Richard Daniel, Soulas-Sprauel Pauline
Atlas d'immunologie Ouvrage
Editions Dunod, 2018, ISBN: 9782100767052.
Résumé | BibTeX | Étiquettes: atlas, cours, ferrandon, immunologie, M3i
@book{Gros2018,
title = {Atlas d'immunologie},
author = {Frédéric Gros and Sylvie Fournel and Samuel Liégeois and Daniel Richard and Pauline Soulas-Sprauel},
editor = {Editions Dunod },
isbn = {9782100767052},
year = {2018},
date = {2018-09-01},
edition = {Editions Dunod},
abstract = {Cet ouvrage présente de façon synthétique et illustrée, les nombreux mécanismes de la réponse immunitaire et les différents types de cellules intervenant dans le système immunitaire.
Les notions de base en immunologie, les modes d'explorations de l'immunité et les applications médicales de l'immunologie et les pathologies sont présentés sous forme de fiches illustrées par de nombreux schémas et photos en couleur.},
keywords = {atlas, cours, ferrandon, immunologie, M3i},
pubstate = {published},
tppubtype = {book}
}
Les notions de base en immunologie, les modes d'explorations de l'immunité et les applications médicales de l'immunologie et les pathologies sont présentés sous forme de fiches illustrées par de nombreux schémas et photos en couleur.
Goto Akira, Okado Kiyoshi, Martins Nelson, Cai Hua, Barbier Vincent, Lamiable Olivier, Troxler Laurent, Santiago Estelle, Kuhn Lauriane, Paik Donggi, Silverman Neal, Holleufer Andreas, Hartmann Rune, Liu Jiyong, Peng Tao, Hoffmann Jules A, Meignin Carine, Daeffler Laurent, Imler Jean-Luc
The Kinase IKKβ Regulates a STING- and NF-κB-Dependent Antiviral Response Pathway in Drosophila Article de journal
Dans: Immunity, no. 49, p. 225-234, 2018.
Résumé | Liens | BibTeX | Étiquettes: hoffmann, imler, M3i, meignin, PPSE
@article{Goto2018,
title = {The Kinase IKKβ Regulates a STING- and NF-κB-Dependent Antiviral Response Pathway in Drosophila},
author = {Akira Goto and Kiyoshi Okado and Nelson Martins and Hua Cai and Vincent Barbier and Olivier Lamiable and Laurent Troxler and Estelle Santiago and Lauriane Kuhn and Donggi Paik and Neal Silverman and Andreas Holleufer and Rune Hartmann and Jiyong Liu and Tao Peng and Jules A Hoffmann and Carine Meignin and Laurent Daeffler and Jean-Luc Imler},
editor = {Elsevier Inc.},
url = {https://doi.org/10.1016/j.immuni.2018.07.013},
doi = {j.immuni.2018.07.013},
year = {2018},
date = {2018-08-21},
journal = {Immunity},
number = {49},
pages = {225-234},
abstract = {Antiviral immunity in Drosophila involves RNA interference and poorly characterized inducible responses. Here, we showed that two components of the IMD pathway, the kinase dIKKβ and the transcription factor Relish, were required to control infection by two picorna-like viruses. We identified a set of genes induced by viral infection and regulated by dIKKβ and Relish, which included an ortholog of STING. We showed that dSTING participated in the control of infection by picorna-like viruses, acting upstream of dIKKβ to regulate expression of Nazo, an antiviral factor. Our data reveal an antiviral function for STING in an animal model devoid of interferons and suggest an evolutionarily ancient role for this molecule in antiviral immunity.},
keywords = {hoffmann, imler, M3i, meignin, PPSE},
pubstate = {published},
tppubtype = {article}
}
J. Bouyer, E. Marois
Pests and vector-borne diseases in the livestock industry Chapitre d'ouvrage
Dans: Academic, Wageningen (Ed.): vol. 5, Chapitre Genetic control of vectors, p. 435–451, 2018, ISBN: 9789086868636 .
Résumé | Liens | BibTeX | Étiquettes: insect resistance, M3i, marois
@inbook{Bouyer2018,
title = {Pests and vector-borne diseases in the livestock industry},
author = {Bouyer J. and Marois E.},
editor = {Wageningen Academic},
url = {https://doi.org/10.3920/978-90-8686-863-6_14 },
doi = {10.3920/978-90-8686-863-6_14},
isbn = {9789086868636 },
year = {2018},
date = {2018-08-15},
volume = {5},
pages = {435–451},
chapter = {Genetic control of vectors},
series = {Ecology and Control of Vector-borne Diseases},
abstract = {In a context of tighter regulations on approved insecticide molecules, the spread of insecticide resistance in insect vectors of human and animal diseases and the introduction of exotic vectors to new territories call for the development of new pest control methods and strategies. New genetic control methods, related to the ancestral sterile insect technique (SIT), show particular promise and are being developed in response to increasing health and agricultural challenges. These include the use of symbionts like Wolbachia and the use of transgenic insect strains, some of which incorporate gene editing techniques that can lead to transgene spread (gene drive). Here we present the principles, associated opportunities and risks, as well as the degree of advancement of these various techniques for a subset of livestock pests and disease vectors including screwworms, tsetse, mosquitoes and stomoxes. We then present some case studies on recent improvements in the use of the SIT in tsetse and the release of insects carrying a dominant lethal gene, symbiont-based approaches and gene drive in mosquitoes. Finally, we call to speed up the development of genetic control, within a rigorous benefit-risk analysis framework including international public consultation.},
keywords = {insect resistance, M3i, marois},
pubstate = {published},
tppubtype = {inbook}
}
Haller Samantha, Franchet Adrien, Hakkim A, Chen J, Drenkard E, Yu S, Schirmeier Steffi, Li Zi, Martins Nelson, Ausubel FM, Liégeois Samuel, Ferrandon Dominique
Quorum-sensing regulator RhlR but not its autoinducer RhlI enables Pseudomonas to evade opsonization Article de journal
Dans: EMBO Reports, 2018.
Liens | BibTeX | Étiquettes: Drosophila, ferrandon, M3i, Opsonin Proteins, Phagocytosis, Pseudomonas aeruginosa, Quorum Sensing
@article{S2018,
title = {Quorum-sensing regulator RhlR but not its autoinducer RhlI enables Pseudomonas to evade opsonization},
author = {Samantha Haller and Adrien Franchet and A Hakkim and J Chen and E Drenkard and S Yu and Steffi Schirmeier and Zi Li and Nelson Martins and FM Ausubel and Samuel Liégeois and Dominique Ferrandon},
url = {http://embor.embopress.org/content/early/2018/03/09/embr.201744880},
doi = {10.15252/embr.201744880},
year = {2018},
date = {2018-03-09},
journal = {EMBO Reports},
keywords = {Drosophila, ferrandon, M3i, Opsonin Proteins, Phagocytosis, Pseudomonas aeruginosa, Quorum Sensing},
pubstate = {published},
tppubtype = {article}
}
Dong Yuemei, Simões Maria L., Marois Eric, Dimopoulos George
CRISPR/Cas9 -mediated gene knockout of Anopheles gambiae FREP1 suppresses malaria parasite infection Article de journal
Dans: PLoS Pathog, vol. 14, no. 3, 2018.
Résumé | Liens | BibTeX | Étiquettes: Anopheles gambiae, CRISPR/Cas9, Knockout, M3i, Malaria, marois
@article{Dong2018,
title = {CRISPR/Cas9 -mediated gene knockout of Anopheles gambiae FREP1 suppresses malaria parasite infection},
author = { Yuemei Dong and Maria L. Simões and Eric Marois and George Dimopoulos },
url = {https://doi.org/10.1371/journal.ppat.1006898},
doi = {10.1371/journal.ppat.1006898},
year = {2018},
date = {2018-03-08},
urldate = {2018-03-08},
journal = {PLoS Pathog},
volume = {14},
number = {3},
abstract = {Plasmodium relies on numerous agonists during its journey through the mosquito vector, and these agonists represent potent targets for transmission-blocking by either inhibiting or interfering with them pre- or post-transcriptionally. The recently developed CRISPR/Cas9-based genome editing tools for Anopheles mosquitoes provide new and promising opportunities for the study of agonist function and for developing malaria control strategies through gene deletion to achieve complete agonist inactivation. Here we have established a modified CRISPR/Cas9 gene editing procedure for the malaria vector Anopheles gambiae, and studied the effect of inactivating the fibrinogen-related protein 1 (FREP1) gene on the mosquito’s susceptibility to Plasmodium and on mosquito fitness. FREP1 knockout mutants developed into adult mosquitoes that showed profound suppression of infection with both human and rodent malaria parasites at the oocyst and sporozoite stages. FREP1 inactivation, however, resulted in fitness costs including a significantly lower blood-feeding propensity, fecundity and egg hatching rate, a retarded pupation time, and reduced longevity after a blood meal.},
keywords = {Anopheles gambiae, CRISPR/Cas9, Knockout, M3i, Malaria, marois},
pubstate = {published},
tppubtype = {article}
}
Issa Najwa, Guillaumot Nina, Lauret Emilie, Matt Nicolas, Scaeffer-Reiss Christine, Dorsselaer Alain Van, Reichhart Jean-Marc, Veillard Florian
The circulating protease Persephone is an immune sensor for microbial proteolytic activities upstream of the Drosophila Toll pathway. Article de journal
Dans: Molecular Cell, vol. 69, no. 4, p. 539-550, 2018, ISSN: 1097-2765.
Résumé | Liens | BibTeX | Étiquettes: circulating protease, immune sensor, M3i, matt, persephone, Protease, proteolitic activities, reichhart, toll pathway
@article{Issa2018,
title = {The circulating protease Persephone is an immune sensor for microbial proteolytic activities upstream of the Drosophila Toll pathway.},
author = {Najwa Issa and Nina Guillaumot and Emilie Lauret and Nicolas Matt and Christine Scaeffer-Reiss and Alain Van Dorsselaer and Jean-Marc Reichhart and Florian Veillard},
url = {http://www.cell.com/molecular-cell/fulltext/S1097-2765(18)30058-3?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1097276518300583%3Fshowall%3Dtrue},
doi = {10.1016/j.molcel.2018.01.029},
issn = {1097-2765},
year = {2018},
date = {2018-02-15},
journal = {Molecular Cell},
volume = {69},
number = {4},
pages = {539-550},
abstract = {Microbial or endogenous molecular patterns as well as pathogen functional features can activate innate immune systems. Whereas detection of infection by pattern recognition receptors has been investigated in details, sensing of virulence factors activities remains less characterized. In Drosophila, genetic evidences indicate that the serine protease Persephone belongs to a danger pathway activated by abnormal proteolytic activities to induce Toll signaling. However, neither the activation mechanism of this pathway nor its specificity has been determined. Here, we identify a unique region in the pro-domain of Persephone that functions as bait for exogenous proteases independently of their origin, type, or specificity. Cleavage in this bait region constitutes the first step of a sequential activation and licenses the subsequent maturation of Persephone to the endogenous cysteine cathepsin 26-29-p. Our results establish Persephone itself as an immune receptor able to sense a broad range of microbes through virulence factor activities rather than molecular patterns.},
keywords = {circulating protease, immune sensor, M3i, matt, persephone, Protease, proteolitic activities, reichhart, toll pathway},
pubstate = {published},
tppubtype = {article}
}
Ferreira Flávia Viana, Aguiar Eric Roberto Guimarães Rocha, Olmo Roenick Proveti, de Oliveira Karla Pollyanna Vieira, Silva Emanuele Guimarães, Sant'Anna Maurício Roberto Viana, de Gontijo Nelder Figueiredo, Kroon Erna Geessien, Imler Jean-Luc, Marques João Trindade
The small non-coding RNA response to virus infection in the Leishmania vector Lutzomyia longipalpis Article de journal
Dans: PLoS Negl Trop Dis, vol. 12, no. 6, p. e0006569, 2018, ISSN: 1935-2735.
Résumé | Liens | BibTeX | Étiquettes: Animals, Host-Pathogen Interactions, imler, Insect Vectors, Leishmania, M3i, ncRNA, Psychodidae, RNA, RNA Interference, Small Interfering, Untranslated, Vesicular stomatitis Indiana virus, Viral
@article{ferreira_small_2018,
title = {The small non-coding RNA response to virus infection in the Leishmania vector Lutzomyia longipalpis},
author = {Flávia Viana Ferreira and Eric Roberto Guimarães Rocha Aguiar and Roenick Proveti Olmo and Karla Pollyanna Vieira de Oliveira and Emanuele Guimarães Silva and Maurício Roberto Viana Sant'Anna and Nelder Figueiredo de Gontijo and Erna Geessien Kroon and Jean-Luc Imler and João Trindade Marques},
doi = {10.1371/journal.pntd.0006569},
issn = {1935-2735},
year = {2018},
date = {2018-01-01},
journal = {PLoS Negl Trop Dis},
volume = {12},
number = {6},
pages = {e0006569},
abstract = {Sandflies are well known vectors for Leishmania but also transmit a number of arthropod-borne viruses (arboviruses). Few studies have addressed the interaction between sandflies and arboviruses. RNA interference (RNAi) mechanisms utilize small non-coding RNAs to regulate different aspects of host-pathogen interactions. The small interfering RNA (siRNA) pathway is a broad antiviral mechanism in insects. In addition, at least in mosquitoes, another RNAi mechanism mediated by PIWI interacting RNAs (piRNAs) is activated by viral infection. Finally, endogenous microRNAs (miRNA) may also regulate host immune responses. Here, we analyzed the small non-coding RNA response to Vesicular stomatitis virus (VSV) infection in the sandfly Lutzoymia longipalpis. We detected abundant production of virus-derived siRNAs after VSV infection in adult sandflies. However, there was no production of virus-derived piRNAs and only mild changes in the expression of vector miRNAs in response to infection. We also observed abundant production of virus-derived siRNAs against two other viruses in Lutzomyia Lulo cells. Together, our results suggest that the siRNA but not the piRNA pathway mediates an antiviral response in sandflies. In agreement with this hypothesis, pre-treatment of cells with dsRNA against VSV was able to inhibit viral replication while knock-down of the central siRNA component, Argonaute-2, led to increased virus levels. Our work begins to elucidate the role of RNAi mechanisms in the interaction between L. longipalpis and viruses and should also open the way for studies with other sandfly-borne pathogens.},
keywords = {Animals, Host-Pathogen Interactions, imler, Insect Vectors, Leishmania, M3i, ncRNA, Psychodidae, RNA, RNA Interference, Small Interfering, Untranslated, Vesicular stomatitis Indiana virus, Viral},
pubstate = {published},
tppubtype = {article}
}
2017
Kuhn Lauriane, Majzoub Karim, Einhorn Evelyne, Chicher Johana, Pompon Julien, Imler Jean-Luc, Hammann Philippe, Meignin Carine
Definition of a RACK1 Interaction Network in Drosophila melanogaster Using SWATH-MS Article de journal
Dans: G3 (Bethesda), 2017, ISSN: 2160-1836.
Résumé | Liens | BibTeX | Étiquettes: imler, M3i, meignin, PPSE
@article{kuhn_definition_2017,
title = {Definition of a RACK1 Interaction Network in Drosophila melanogaster Using SWATH-MS},
author = {Lauriane Kuhn and Karim Majzoub and Evelyne Einhorn and Johana Chicher and Julien Pompon and Jean-Luc Imler and Philippe Hammann and Carine Meignin},
doi = {10.1534/g3.117.042564},
issn = {2160-1836},
year = {2017},
date = {2017-12-31},
journal = {G3 (Bethesda)},
abstract = {Receptor for Activated C kinase 1 (RACK1) is a scaffold protein that has been found in association with several signaling complexes, and with the 40S subunit of the ribosome. Using the model organism Drosophila melanogaster, we recently showed that RACK1 is required at the ribosome for IRES-mediated translation of viruses. Here, we report a proteomic characterization of the interactome of RACK1 in Drosophila S2 cells. We carried out Label-Free quantitation using both Data-Dependent and Data-Independent Acquisition and observed a significant advantage for the Sequential Window Acquisition of all THeoretical fragment-ion spectra (SWATH) method both in terms of identification of interactants and quantification of low abundance proteins. These data represent the first SWATH spectral library available for Drosophila and will be a useful resource for the community. A total of 52 interacting proteins were identified, including several molecules involved in translation such as structural components of the ribosome, factors regulating translation initiation or elongation and RNA binding proteins. Among these 52 proteins, 15 were identified as partners by the SWATH strategy only. Interestingly, these 15 proteins are significantly enriched for the functions translation and nucleic acid binding. This enrichment reflects the engagement of RACK1 at the ribosome and highlights the added value of SWATH analysis. A functional screen did not reveal any protein sharing the interesting properties of RACK1, which is required for IRES-dependent translation and not essential for cell viability. Intriguingly however, 10 of the RACK1 partners identified restrict replication of Cricket paralysis virus, an IRES-containing virus.},
keywords = {imler, M3i, meignin, PPSE},
pubstate = {published},
tppubtype = {article}
}
G Triller, SW Scally, G Costa, M Pissarev, C Kreschel, A Bosch, E Marois, BK Sack, R Murugan, AM Salman, CJ Janse, SM Khan, SH Kappe, AA Adegnika, B Mordmüller, EA Levashina, JP Julien, H Wardemann
Natural parasite exposure induces protective human anti-malarial antibodies Article de journal
Dans: Immunity , vol. 47, iss. 6, p. 1197-1209, 2017.
Résumé | Liens | BibTeX | Étiquettes: anti-malarial antibodie, M3i, marois
@article{Triller2017,
title = {Natural parasite exposure induces protective human anti-malarial antibodies},
author = {Triller G and Scally SW and Costa G and Pissarev M and Kreschel C and Bosch A and Marois E and Sack BK and Murugan R and Salman AM and Janse CJ and Khan SM and Kappe SH and Adegnika AA and Mordmüller B and Levashina EA and Julien JP and Wardemann H},
url = {https://doi.org/10.1016/j.immuni.2017.11.007},
doi = {10.1016/j.immuni.2017.11.007},
year = {2017},
date = {2017-12-19},
journal = {Immunity },
volume = {47},
issue = {6},
pages = {1197-1209},
abstract = {Antibodies against the NANP repeat of circumsporozoite protein (CSP), the major surface antigen of Plasmodium falciparum (Pf) sporozoites, can protect from malaria in animal models but protective humoral immunity is difficult to induce in humans. Here we cloned and characterized rare affinity-matured human NANP-reactive memory B cell antibodies elicited by natural Pf exposure that potently inhibited parasite transmission and development in vivo. We unveiled the molecular details of antibody binding to two distinct protective epitopes within the NANP repeat. NANP repeat recognition was largely mediated by germline encoded and immunoglobulin (Ig) heavy-chain complementarity determining region 3 (HCDR3) residues, whereas affinity maturation contributed predominantly to stabilizing the antigen-binding site conformation. Combined, our findings illustrate the power of exploring human anti-CSP antibody responses to develop tools for malaria control in the mammalian and the mosquito vector and provide a molecular basis for the structure-based design of next-generation CSP malaria vaccines. },
keywords = {anti-malarial antibodie, M3i, marois},
pubstate = {published},
tppubtype = {article}
}
Gross E, Vincens Q, Einhorn E, Noireterre A, Schaeffer L, Kuhn L, Imler JL, Eriani, Meignin C, Martin F
The IRES5'UTR of the dicistrovirus cricket paralysis virus is a type III IRES containing an essential pseudoknot structure Article de journal
Dans: Nucleic Acids Research, vol. 45, no. 15, p. 8993-9004, 2017.
Résumé | Liens | BibTeX | Étiquettes: imler, IRES, M3i, meignin
@article{Gross_2017,
title = {The IRES5'UTR of the dicistrovirus cricket paralysis virus is a type III IRES containing an essential pseudoknot structure},
author = {E Gross and Q Vincens and E Einhorn and A Noireterre and L Schaeffer and L Kuhn and JL Imler and Eriani and C Meignin and F Martin},
url = {https://academic.oup.com/nar/article/45/15/8993/3978035},
doi = {10.1093/nar/gkx622 },
year = {2017},
date = {2017-09-06},
journal = {Nucleic Acids Research},
volume = {45},
number = {15},
pages = {8993-9004},
abstract = {Cricket paralysis virus (CrPV) is a dicistrovirus. Its positive-sense single-stranded RNA genome contains two internal ribosomal entry sites (IRESs). The 5' untranslated region (5'UTR) IRES5'UTR mediates translation of non-structural proteins encoded by ORF1 whereas the well-known intergenic region (IGR) IRESIGR is required for translation of structural proteins from open reading frame 2 in the late phase of infection. Concerted action of both IRES is essential for host translation shut-off and viral translation. IRESIGR has been extensively studied, in contrast the IRES5'UTR remains largely unexplored. Here, we define the minimal IRES element required for efficient translation initiation in drosophila S2 cell-free extracts. We show that IRES5'UTR promotes direct recruitment of the ribosome on the cognate viral AUG start codon without any scanning step, using a Hepatitis-C virus-related translation initiation mechanism. Mass spectrometry analysis revealed that IRES5'UTR recruits eukaryotic initiation factor 3, confirming that it belongs to type III class of IRES elements. Using Selective 2'-hydroxyl acylation analyzed by primer extension and DMS probing, we established a secondary structure model of 5'UTR and of the minimal IRES5'UTR. The IRES5'UTR contains a pseudoknot structure that is essential for proper folding and ribosome recruitment. Overall, our results pave the way for studies addressing the synergy and interplay between the two IRES from CrPV. },
keywords = {imler, IRES, M3i, meignin},
pubstate = {published},
tppubtype = {article}
}
FM Cao C Cogni R Barbier V Jiggins
Complex Coding and Regulatory Polymorphisms in a Restriction Factor Determine the Susceptibility of Drosophila to Viral Infection Article de journal
Dans: Genetics, vol. 206, p. 2159-2173, 2017.
Résumé | Liens | BibTeX | Étiquettes: imler, M3i
@article{FM2017,
title = {Complex Coding and Regulatory Polymorphisms in a Restriction Factor Determine the Susceptibility of Drosophila to Viral Infection},
author = {Cao C
Cogni R
Barbier V
Jiggins FM},
url = {https://academic.oup.com/genetics/article/206/4/2159/6072650},
doi = {10.1534/genetics.117.201970},
year = {2017},
date = {2017-06-19},
journal = {Genetics},
volume = {206},
pages = {2159-2173},
abstract = {It is common to find that major-effect genes are an important cause of variation in susceptibility to infection. Here we have characterized natural variation in a gene called pastrel that explains over half of the genetic variance in susceptibility to the Drosophila C virus (DCV) in populations of Drosophila melanogaster. We found extensive allelic heterogeneity, with a sample of seven alleles of pastrel from around the world conferring four phenotypically distinct levels of resistance. By modifying candidate SNPs in transgenic flies, we show that the largest effect is caused by an amino acid polymorphism that arose when an ancestral threonine was mutated to alanine, greatly increasing resistance to DCV. Overexpression of the ancestral, susceptible allele provides strong protection against DCV; indicating that this mutation acted to improve an existing restriction factor. The pastrel locus also contains complex structural variation and cis-regulatory polymorphisms altering gene expression. We find that higher expression of pastrel is associated with increased survival after DCV infection. To understand why this variation is maintained in populations, we investigated genetic variation surrounding the amino acid variant that is causing flies to be resistant. We found no evidence of natural selection causing either recent changes in allele frequency or geographical variation in frequency, suggesting that this is an old polymorphism that has been maintained at a stable frequency. Overall, our data demonstrate how complex genetic variation at a single locus can control susceptibility to a virulent natural pathogen.},
keywords = {imler, M3i},
pubstate = {published},
tppubtype = {article}
}
Volohonsky Gloria, Hopp Ann-Katrin, Saenger Mélanie, Soichot Julien, Scholze Heidi, Boch Jens, Blandin Stéphanie A, Marois Eric
Transgenic Expression of the Anti-parasitic Factor TEP1 in the Malaria Mosquito Anopheles gambiae Article de journal
Dans: PLOS Pathogens, vol. 13, no. 1, p. e1006113, 2017, ISSN: 1553-7374.
Liens | BibTeX | Étiquettes: Anopheles gambiae, anti-parasitic factor, blandin, M3i, Malaria, marois, TEP1, transgenic
@article{volohonsky_transgenic_2017,
title = {Transgenic Expression of the Anti-parasitic Factor TEP1 in the Malaria Mosquito Anopheles gambiae},
author = {Gloria Volohonsky and Ann-Katrin Hopp and Mélanie Saenger and Julien Soichot and Heidi Scholze and Jens Boch and Stéphanie A Blandin and Eric Marois},
editor = {Kenneth D Vernick},
url = {http://dx.plos.org/10.1371/journal.ppat.1006113},
doi = {10.1371/journal.ppat.1006113},
issn = {1553-7374},
year = {2017},
date = {2017-01-01},
urldate = {2017-02-01},
journal = {PLOS Pathogens},
volume = {13},
number = {1},
pages = {e1006113},
keywords = {Anopheles gambiae, anti-parasitic factor, blandin, M3i, Malaria, marois, TEP1, transgenic},
pubstate = {published},
tppubtype = {article}
}
Mussabekova Assel, Daeffler Laurent, Imler Jean-Luc
Innate and intrinsic antiviral immunity in Drosophila Article de journal
Dans: Cell. Mol. Life Sci., 2017, ISSN: 1420-9071.
Résumé | Liens | BibTeX | Étiquettes: Argonaute 2, Dicer-2, IMD pathway, imler, Jak/STAT pathway, M3i, NF-κB
@article{mussabekova_innate_2017,
title = {Innate and intrinsic antiviral immunity in Drosophila},
author = {Assel Mussabekova and Laurent Daeffler and Jean-Luc Imler},
doi = {10.1007/s00018-017-2453-9},
issn = {1420-9071},
year = {2017},
date = {2017-01-01},
journal = {Cell. Mol. Life Sci.},
abstract = {The fruit fly Drosophila melanogaster has been a valuable model to investigate the genetic mechanisms of innate immunity. Initially focused on the resistance to bacteria and fungi, these studies have been extended to include antiviral immunity over the last decade. Like all living organisms, insects are continually exposed to viruses and have developed efficient defense mechanisms. We review here our current understanding on antiviral host defense in fruit flies. A major antiviral defense in Drosophila is RNA interference, in particular the small interfering (si) RNA pathway. In addition, complex inducible responses and restriction factors contribute to the control of infections. Some of the genes involved in these pathways have been conserved through evolution, highlighting loci that may account for susceptibility to viral infections in humans. Other genes are not conserved and represent species-specific innovations.},
keywords = {Argonaute 2, Dicer-2, IMD pathway, imler, Jak/STAT pathway, M3i, NF-κB},
pubstate = {published},
tppubtype = {article}
}
Koltun Bella, Shackelford Eliza, Bonnay François, Matt Nicolas, Reichhart Jean-Marc, Orian Amir
The SUMO-targeted ubiquitin ligase, Dgrn, is essential for Drosophila innate immunity Article de journal
Dans: The International Journal of Developmental Biology, vol. 61, no. 3-4-5, p. 319–327, 2017, ISSN: 0214-6282.
Liens | BibTeX | Étiquettes: Dgrn, Drosophila, innate immunity, Ligase, M3i, matt, reichhart, SUMO, target, ubiquitin
@article{koltun_sumo-targeted_2017,
title = {The SUMO-targeted ubiquitin ligase, Dgrn, is essential for Drosophila innate immunity},
author = {Bella Koltun and Eliza Shackelford and François Bonnay and Nicolas Matt and Jean-Marc Reichhart and Amir Orian},
url = {http://www.intjdevbiol.com/paper.php?doi=160250ao},
doi = {10.1387/ijdb.160250ao},
issn = {0214-6282},
year = {2017},
date = {2017-01-01},
urldate = {2017-07-12},
journal = {The International Journal of Developmental Biology},
volume = {61},
number = {3-4-5},
pages = {319--327},
keywords = {Dgrn, Drosophila, innate immunity, Ligase, M3i, matt, reichhart, SUMO, target, ubiquitin},
pubstate = {published},
tppubtype = {article}
}
Chamy Laure El, Matt Nicolas, Reichhart Jean-Marc
Advances in Myeloid-Like Cell Origins and Functions in the Model Organism Drosophila melanogaster Article de journal
Dans: Microbiology Spectrum, vol. 5, no. 1, 2017, ISSN: 2165-0497.
Liens | BibTeX | Étiquettes: Drosophila melanogaster, M3i, matt, Myeloid-Like Cell Origins, reichhart
@article{Chamy2017,
title = {Advances in Myeloid-Like Cell Origins and Functions in the Model Organism Drosophila melanogaster},
author = {Laure El Chamy and Nicolas Matt and Jean-Marc Reichhart},
url = {http://www.asmscience.org/content/journal/microbiolspec/10.1128/microbiolspec.MCHD-0038-2016},
doi = {10.1128/microbiolspec.MCHD-0038-2016},
issn = {2165-0497},
year = {2017},
date = {2017-01-01},
urldate = {2017-07-12},
journal = {Microbiology Spectrum},
volume = {5},
number = {1},
keywords = {Drosophila melanogaster, M3i, matt, Myeloid-Like Cell Origins, reichhart},
pubstate = {published},
tppubtype = {article}
}
2016
Lee Kwang-Zin, Lestradet Matthieu, Socha Catherine, Schirmeier Stefanie, Schmitz Antonin, Spenlé Caroline, Lefebvre Olivier, Keime Céline, Yamba Wennida M., Bou Aoun Richard, Liegeois Samuel, Schwab Yannick, Simon-Assmann Patricia, Dalle Frédéric, Ferrandon Dominique
Enterocyte Purge and Rapid Recovery Is a Resilience Reaction of the Gut Epithelium to Pore-Forming Toxin Attack Article de journal
Dans: Cell Host & Microbe, 2016, ISSN: 1931-3128.
Résumé | Liens | BibTeX | Étiquettes: Epithelium, ferrandon, gut, M3i, resilience
@article{Lee2016,
title = {Enterocyte Purge and Rapid Recovery Is a Resilience Reaction of the Gut Epithelium to Pore-Forming Toxin Attack},
author = {Kwang-Zin Lee and Matthieu Lestradet and Catherine Socha and Stefanie Schirmeier and Antonin Schmitz and Caroline Spenlé and Olivier Lefebvre and Céline Keime and Wennida M. Yamba and Richard Bou Aoun and Samuel Liegeois and Yannick Schwab and Patricia Simon-Assmann and Frédéric Dalle and Dominique Ferrandon},
editor = {L Abate},
url = {http://www.sciencedirect.com/science/article/pii/S193131281630436X},
doi = {10.1016/j.chom.2016.10.010},
issn = {1931-3128},
year = {2016},
date = {2016-11-23},
urldate = {2016-11-25},
journal = {Cell Host & Microbe},
abstract = {Summary
Besides digesting nutrients, the gut protects the host against invasion by pathogens. Enterocytes may be subjected to damage by both microbial and host defensive responses, causing their death. Here, we report a rapid epithelial response that alleviates infection stress and protects the enterocytes from the action of microbial virulence factors. Intestinal epithelia exposed to hemolysin, a pore-forming toxin secreted by Serratia marcescens, undergo an evolutionarily conserved process of thinning followed by the recovery of their initial thickness within a few hours. In response to hemolysin attack, Drosophila melanogaster enterocytes extrude most of their apical cytoplasm, including damaged organelles such as mitochondria, yet do not lyse. We identify two secreted peptides, the expression of which requires CyclinJ, that mediate the recovery phase in which enterocytes regain their original shape and volume. Epithelial thinning and recovery constitute a fast and efficient response to intestinal infections, with pore-forming toxins acting as alarm signals.},
keywords = {Epithelium, ferrandon, gut, M3i, resilience},
pubstate = {published},
tppubtype = {article}
}
Besides digesting nutrients, the gut protects the host against invasion by pathogens. Enterocytes may be subjected to damage by both microbial and host defensive responses, causing their death. Here, we report a rapid epithelial response that alleviates infection stress and protects the enterocytes from the action of microbial virulence factors. Intestinal epithelia exposed to hemolysin, a pore-forming toxin secreted by Serratia marcescens, undergo an evolutionarily conserved process of thinning followed by the recovery of their initial thickness within a few hours. In response to hemolysin attack, Drosophila melanogaster enterocytes extrude most of their apical cytoplasm, including damaged organelles such as mitochondria, yet do not lyse. We identify two secreted peptides, the expression of which requires CyclinJ, that mediate the recovery phase in which enterocytes regain their original shape and volume. Epithelial thinning and recovery constitute a fast and efficient response to intestinal infections, with pore-forming toxins acting as alarm signals.
Ehrhardt Katharina, Deregnaucourt Christiane, Goetz Alice-Anne, Tzanova Tzvetomira, Gallo Valentina, Arese Paolo, Pradines Bruno, Adjalley Sophie H, Bagrel Denyse, Blandin Stephanie A, Lanzer Michael, Davioud-Charvet Elisabeth
The redox-cycler plasmodione is a fast acting antimalarial lead compound with pronounced activity against sexual and early asexual blood-stage parasites Article de journal
Dans: Antimicrob. Agents Chemother., vol. 60, no. 9, p. 5146-5158, 2016, ISSN: 1098-6596.
Résumé | Liens | BibTeX | Étiquettes: antimalarial, blandin, M3i, parasites, Plasmodium, redox-cycler
@article{ehrhardt_redox-cycler_2016,
title = {The redox-cycler plasmodione is a fast acting antimalarial lead compound with pronounced activity against sexual and early asexual blood-stage parasites},
author = {Katharina Ehrhardt and Christiane Deregnaucourt and Alice-Anne Goetz and Tzvetomira Tzanova and Valentina Gallo and Paolo Arese and Bruno Pradines and Sophie H Adjalley and Denyse Bagrel and Stephanie A Blandin and Michael Lanzer and Elisabeth Davioud-Charvet},
url = {http://aac.asm.org/content/60/9/5146},
doi = {10.1128/AAC.02975-15},
issn = {1098-6596},
year = {2016},
date = {2016-09-01},
journal = {Antimicrob. Agents Chemother.},
volume = {60},
number = {9},
pages = {5146-5158},
abstract = {Previously, we presented the chemical design of a promising series of antimalarial agents, 3-[substituted-benzyl]-menadiones, with potent in vitro and in vivo activities. Ongoing studies on the mode of action of antimalarial 3-[substituted-benzyl]-menadiones revealed that these agents disturb the redox balance of the parasitized erythrocyte by acting as redox-cyclers - a strategy that is broadly recognized for the development of new antimalarial agents. Here, we report a detailed parasitological characterization of the in vitro activity profile of the lead compound 3-[4-(trifluoromethyl)benzyl]-menadione 1c (henceforth called plasmodione) against intra-erythrocytic stages of the human malaria parasite Plasmodium falciparum We show that plasmodione acts rapidly against asexual blood stages, thereby disrupting the clinically relevant intra-erythrocytic life cycle of the parasite, and furthermore has potent activity against early gametocytes. The lead's antiplasmodial activity was unaffected by the most common resistance mechanisms to clinically used antimalarials. Moreover, plasmodione has a low potential to induce drug resistance and a fast killing speed as observed by culturing parasites under continuous drug pressure. Drug interactions with licensed antimalarial drugs were also established using the fixed-ratio isobologram method. Initial toxicological profiling suggests that it is a safe agent for possible human use. Our studies identify plasmodione as a promising antimalarial lead compound and strongly support the future development of redox-active benzylmenadiones as antimalarial agents.},
keywords = {antimalarial, blandin, M3i, parasites, Plasmodium, redox-cycler},
pubstate = {published},
tppubtype = {article}
}
Paro Simona, Imler Jean-Luc
Encyclopedia of Immunobiology Chapitre d'ouvrage
Dans: Ratcliffe, M (Ed.): vol. 1, Chapitre “Immunity in insects”, p. 454-461, Elsevier, 2016.
BibTeX | Étiquettes: imler, Immunity, Insect, M3i
@inbook{Paro0000,
title = {Encyclopedia of Immunobiology},
author = {Simona Paro and Jean-Luc Imler},
editor = {M Ratcliffe},
year = {2016},
date = {2016-08-01},
volume = {1},
pages = {454-461},
publisher = {Elsevier},
chapter = {“Immunity in insects”},
keywords = {imler, Immunity, Insect, M3i},
pubstate = {published},
tppubtype = {inbook}
}
Lamiable Olivier, Kellenberger Christine, Kemp Cordula, Troxler Laurent, Pelte Nadège, Boutros Michael, Marques Joao Trindade, Daeffler Laurent, Hoffmann Jules A, Roussel Alain, Imler Jean-Luc
Cytokine Diedel and a viral homologue suppress the IMD pathway in Drosophila Article de journal
Dans: PNAS, vol. 113, no. 3, p. 698–703, 2016, ISSN: 0027-8424, 1091-6490.
Résumé | Liens | BibTeX | Étiquettes: antiviral immunity, bioinformatic, cytokine, Edin, hoffmann, imler, M3i, Sindbis Virus, virokine
@article{lamiable_cytokine_2016,
title = {Cytokine Diedel and a viral homologue suppress the IMD pathway in Drosophila},
author = {Olivier Lamiable and Christine Kellenberger and Cordula Kemp and Laurent Troxler and Nadège Pelte and Michael Boutros and Joao Trindade Marques and Laurent Daeffler and Jules A Hoffmann and Alain Roussel and Jean-Luc Imler},
url = {http://www.pnas.org/content/113/3/698.abstract},
doi = {10.1073/pnas.1516122113},
issn = {0027-8424, 1091-6490},
year = {2016},
date = {2016-01-19},
urldate = {2016-01-07},
journal = {PNAS},
volume = {113},
number = {3},
pages = {698–703},
abstract = {Viruses are obligatory intracellular parasites that suffer strong evolutionary pressure from the host immune system. Rapidly evolving viral genomes can adapt to this pressure by acquiring genes that counteract host defense mechanisms. For example, many vertebrate DNA viruses have hijacked cellular genes encoding cytokines or cytokine receptors to disrupt host cell communication. Insect viruses express suppressors of RNA interference or apoptosis, highlighting the importance of these cell intrinsic antiviral mechanisms in invertebrates. Here, we report the identification and characterization of a family of proteins encoded by insect DNA viruses that are homologous to a 12-kDa circulating protein encoded by the virus-induced Drosophila gene diedel (die). We show that die mutant flies have shortened lifespan and succumb more rapidly than controls when infected with Sindbis virus. This reduced viability is associated with deregulated activation of the immune deficiency (IMD) pathway of host defense and can be rescued by mutations in the genes encoding the homolog of IKKγ or IMD itself. Our results reveal an endogenous pathway that is exploited by insect viruses to modulate NF-κB signaling and promote fly survival during the antiviral response.},
keywords = {antiviral immunity, bioinformatic, cytokine, Edin, hoffmann, imler, M3i, Sindbis Virus, virokine},
pubstate = {published},
tppubtype = {article}
}
Lamiable Olivier, Arnold Johan, da de Faria Isaque Joao Silva, Olmo Roenick Proveti, Bergami Francesco, Meignin Carine, Hoffmann Jules A, Marques Joao Trindade, Imler Jean-Luc
Analysis of the Contribution of Hemocytes and Autophagy to Drosophila Antiviral Immunity Article de journal
Dans: J. Virol., vol. 90, no. 11, p. 5415–5426, 2016, ISSN: 0022-538X, 1098-5514.
Liens | BibTeX | Étiquettes: antiviral immunity, Autophagy, Hemocytes, hoffmann, imler, M3i, meignin
@article{lamiable_analysis_2016,
title = {Analysis of the Contribution of Hemocytes and Autophagy to Drosophila Antiviral Immunity},
author = {Olivier Lamiable and Johan Arnold and Isaque Joao Silva da de Faria and Roenick Proveti Olmo and Francesco Bergami and Carine Meignin and Jules A Hoffmann and Joao Trindade Marques and Jean-Luc Imler},
url = {http://jvi.asm.org/content/90/11/5415},
doi = {10.1128/JVI.00238-16},
issn = {0022-538X, 1098-5514},
year = {2016},
date = {2016-01-01},
urldate = {2016-06-05},
journal = {J. Virol.},
volume = {90},
number = {11},
pages = {5415--5426},
keywords = {antiviral immunity, Autophagy, Hemocytes, hoffmann, imler, M3i, meignin},
pubstate = {published},
tppubtype = {article}
}
Marques João T, Imler Jean-Luc
The diversity of insect antiviral immunity: insights from viruses Article de journal
Dans: Current Opinion in Microbiology, vol. 32, p. 71–76, 2016, ISSN: 1369-5274.
Résumé | Liens | BibTeX | Étiquettes: imler, M3i
@article{marques_diversity_2016,
title = {The diversity of insect antiviral immunity: insights from viruses},
author = {João T Marques and Jean-Luc Imler},
url = {http://www.sciencedirect.com/science/article/pii/S1369527416300571},
doi = {10.1016/j.mib.2016.05.002},
issn = {1369-5274},
year = {2016},
date = {2016-01-01},
urldate = {2016-06-05},
journal = {Current Opinion in Microbiology},
volume = {32},
pages = {71--76},
abstract = {Insects represent over 70% of all animal species. Recent virome analyses reveal unprecedented genetic diversity of insect viruses, which appears to match that of their hosts. Thus, insect-virus interactions may provide information on a vast repertoire of antiviral immune mechanisms. Tapping into this diversity is challenging because of several constraints imposed by the uniqueness of each insect model. Nevertheless, it is clear that many conserved and divergent pathways participate in the control of viral infection in insects. Co-evolution between hosts and viruses favors the development of immune evasion mechanisms by the pathogen. Viral suppressors can offer unique perspective on host pathways and emphasize the importance of RNA interference, apoptosis, but also NF-κB pathways and translation control in insect antiviral immunity.},
keywords = {imler, M3i},
pubstate = {published},
tppubtype = {article}
}
Martins Nelson, Imler Jean-Luc, Meignin Carine
Discovery of novel targets for antivirals: learning from flies Article de journal
Dans: Curr Opin Virol, vol. 20, p. 64–70, 2016, ISSN: 1879-6265.
Résumé | Liens | BibTeX | Étiquettes: antiviral, imler, M3i, meignin, target
@article{martins_discovery_2016,
title = {Discovery of novel targets for antivirals: learning from flies},
author = {Nelson Martins and Jean-Luc Imler and Carine Meignin},
url = {http://www.sciencedirect.com/science/article/pii/S1879625716301274},
doi = {10.1016/j.coviro.2016.09.005},
issn = {1879-6265},
year = {2016},
date = {2016-01-01},
journal = {Curr Opin Virol},
volume = {20},
pages = {64--70},
abstract = {Developing antiviral drugs is challenging due to the small number of targets in viruses, and the rapid evolution of viral genes. Animals have evolved a number of efficient antiviral defence mechanisms, which can serve as a source of inspiration for novel therapies. The genetically tractable insect Drosophila belongs to the most diverse group of animals. Genetic and transcriptomic analyses have recently identified Drosophila genes encoding viral restriction factors. Some of them represent evolutionary novelties and their characterization may provide hints for the design of directly acting antivirals. In addition, functional screens revealed conserved host factors required for efficient viral translation, such as the ribosomal protein RACK1 and the release factor Pelo. These proteins are promising candidates for host-targeted antivirals.},
keywords = {antiviral, imler, M3i, meignin, target},
pubstate = {published},
tppubtype = {article}
}
Lamiable Olivier, Meignin Carine, Imler Jean-Luc
WntD and Diedel: Two immunomodulatory cytokines in Drosophila immunity Article de journal
Dans: Fly (Austin), vol. 10, no. 4, p. 187–194, 2016, ISSN: 1933-6942.
Résumé | Liens | BibTeX | Étiquettes: Cytokines, Drosophila, IMD pathway, imler, innate immunity, M3i, meignin, virus
@article{lamiable_wntd_2016,
title = {WntD and Diedel: Two immunomodulatory cytokines in Drosophila immunity},
author = {Olivier Lamiable and Carine Meignin and Jean-Luc Imler},
url = {http://www.tandfonline.com/doi/abs/10.1080/19336934.2016.1202387?journalCode=kfly20},
doi = {10.1080/19336934.2016.1202387},
issn = {1933-6942},
year = {2016},
date = {2016-01-01},
journal = {Fly (Austin)},
volume = {10},
number = {4},
pages = {187--194},
abstract = {Remarkable progress has been made on the understanding of the basic mechanisms of innate immunity in flies, from sensing infection to production of effector molecules. However, how the immune response is orchestrated at the level of the organism remains poorly understood. While cytokines activating immune responses, such as Spaetzle or Unpaired-3, have been identified and characterized in Drosophila, much less is known regarding immunosuppressor cytokines. In a recent publication, we reported the identification of a novel cytokine, Diedel, which acts as systemic negative regulator of the IMD pathway. Here, we discuss the similarities between Diedel and WntD, another immunomodulatory cytokine and present evidence that the 2 molecules act independently from one another.},
keywords = {Cytokines, Drosophila, IMD pathway, imler, innate immunity, M3i, meignin, virus},
pubstate = {published},
tppubtype = {article}
}
Baron Olga Lucia, Deleury Emeline, Reichhart Jean-Marc, Coustau Christine
The LBP/BPI multigenic family in invertebrates: Evolutionary history and evidences of specialization in mollusks Article de journal
Dans: Developmental & Comparative Immunology, vol. 57, p. 20–30, 2016, ISSN: 0145305X.
Liens | BibTeX | Étiquettes: BPI, Evolution, Invertebrate, LBP, M3i, mollusks, multigenic family, reichhart
@article{baron_lbp/bpi_2016,
title = {The LBP/BPI multigenic family in invertebrates: Evolutionary history and evidences of specialization in mollusks},
author = {Olga Lucia Baron and Emeline Deleury and Jean-Marc Reichhart and Christine Coustau},
url = {http://linkinghub.elsevier.com/retrieve/pii/S0145305X15300756},
doi = {10.1016/j.dci.2015.11.006},
issn = {0145305X},
year = {2016},
date = {2016-01-01},
urldate = {2017-07-12},
journal = {Developmental & Comparative Immunology},
volume = {57},
pages = {20--30},
keywords = {BPI, Evolution, Invertebrate, LBP, M3i, mollusks, multigenic family, reichhart},
pubstate = {published},
tppubtype = {article}
}
Enkler L, Richer D, Marchand A L, Ferrandon D, Jossinet F
Genome engineering in the yeast pathogen Candida glabrata using the CRISPR-Cas9 system. Article de journal
Dans: Sci Rep, vol. 6, p. 35766, 2016, ISBN: 27767081.
Résumé | Liens | BibTeX | Étiquettes: JOSSINET, M3i, Unité ARN
@article{,
title = {Genome engineering in the yeast pathogen Candida glabrata using the CRISPR-Cas9 system.},
author = {L Enkler and D Richer and A L Marchand and D Ferrandon and F Jossinet},
url = {https://www.ncbi.nlm.nih.gov/pubmed/27767081?dopt=Abstract},
doi = {10.1038/srep35766},
isbn = {27767081},
year = {2016},
date = {2016-01-01},
journal = {Sci Rep},
volume = {6},
pages = {35766},
abstract = {Among Candida species, the opportunistic fungal pathogen Candida glabrata has become the second most common causative agent of candidiasis in the world and a major public health concern. Yet, few molecular tools and resources are available to explore the biology of C. glabrata and to better understand its virulence during infection. In this study, we describe a robust experimental strategy to generate loss-of-function mutants in C. glabrata. The procedure is based on the development of three main tools: (i) a recombinant strain of C. glabrata constitutively expressing the CRISPR-Cas9 system, (ii) an online program facilitating the selection of the most efficient guide RNAs for a given C. glabrata gene, and (iii) the identification of mutant strains by the Surveyor technique and sequencing. As a proof-of-concept, we have tested the virulence of some mutants in vivo in a Drosophila melanogaster infection model. Our results suggest that yps11 and a previously uncharacterized serine/threonine kinase are involved, directly or indirectly, in the ability of the pathogenic yeast to infect this model host organism.},
keywords = {JOSSINET, M3i, Unité ARN},
pubstate = {published},
tppubtype = {article}
}
2015
Veillard Florian, Troxler Laurent, Reichhart Jean-Marc
Drosophila melanogaster clip-domain serine proteases: Structure, function and regulation Article de journal
Dans: Biochimie, vol. 122, p. 255-269, 2015, ISBN: 0300-9084.
Résumé | Liens | BibTeX | Étiquettes: bioinformatic, Chymotrypsin, clip domain, Immunity, Insect, M3i, Melanization, reichhart, Serine Proteases, Serpin, Toll
@article{veillard_drosophila_2015,
title = {Drosophila melanogaster clip-domain serine proteases: Structure, function and regulation},
author = {Florian Veillard and Laurent Troxler and Jean-Marc Reichhart},
url = {http://www.sciencedirect.com/science/article/pii/S030090841500317X},
doi = {10.1016/j.biochi.2015.10.007},
isbn = {0300-9084},
year = {2015},
date = {2015-10-08},
journal = {Biochimie},
volume = {122},
pages = {255-269},
abstract = {Mammalian chymotrypsin-like serine proteases (SPs) are one of the best-studied family of enzymes with roles in a wide range of physiological processes, including digestion, blood coagulation, fibrinolysis and humoral immunity. Extracellular SPs can form cascades, in which one protease activates the zymogen of the next protease in the chain, to amplify physiological or pathological signals. These extracellular SPs are generally multi-domain proteins, with pro-domains that are involved in protein–protein interactions critical for the sequential organization of the cascades, the control of their intensity and their proper localization. Far less is known about invertebrate SPs than their mammalian counterparts. In insect genomes, SPs and their proteolytically inactive homologs (SPHs) constitute large protein families. In addition to the chymotrypsin fold, many of these proteins contain additional structural domains, often with conserved mammalian orthologues. However, the largest group of arthropod SP regulatory modules is the clip domains family, which has only been identified in arthropods. The clip-domain SPs are extracellular and have roles in the immune response and embryonic development. The powerful reverse-genetics tools in Drosophila melanogaster have been essential to identify the functions of clip-SPs and their organization in sequential cascades. This review focuses on the current knowledge of Drosophila clip-SPs and presents, when necessary, data obtained in other insect models. We will first cover the biochemical and structural features of clip domain SPs and SPHs. Clip-SPs are implicated in three main biological processes: the control of the dorso-ventral patterning during embryonic development; the activation of the Toll-mediated response to microbial infections and the prophenoloxydase cascade, which triggers melanization. Finally, we review the regulation of SPs and SPHs, from specificity of activation to inhibition by endogenous or pathogen-encoded inhibitors.},
keywords = {bioinformatic, Chymotrypsin, clip domain, Immunity, Insect, M3i, Melanization, reichhart, Serine Proteases, Serpin, Toll},
pubstate = {published},
tppubtype = {article}
}
Chamy Laure El, Matt Nicolas, Ntwasa Monde, Reichhart Jean-Marc
The multilayered innate immune defense of the gut Article de journal
Dans: Biomed J, vol. 38, no. 4, p. 276–284, 2015, ISSN: 2320-2890.
Résumé | Liens | BibTeX | Étiquettes: fly, gut, innate immunity, M3i, matt, reichhart
@article{el_chamy_multilayered_2015,
title = {The multilayered innate immune defense of the gut},
author = {Laure El Chamy and Nicolas Matt and Monde Ntwasa and Jean-Marc Reichhart},
url = {http://www.biomedj.org/text.asp?2015/38/4/276/158621},
doi = {10.4103/2319-4170.158621},
issn = {2320-2890},
year = {2015},
date = {2015-08-01},
journal = {Biomed J},
volume = {38},
number = {4},
pages = {276--284},
abstract = {In the wild, the fruit fly Drosophila melanogaster thrives on rotten fruit. The digestive tract maintains a powerful gut immune barrier to regulate the ingested microbiota, including entomopathogenic bacteria. This gut immune barrier includes a chitinous peritrophic matrix that isolates the gut contents from the epithelial cells. In addition, the epithelial cells are tightly sealed by septate junctions and can mount an inducible immune response. This local response can be activated by invasive bacteria, or triggered by commensal bacteria in the gut lumen. As with chronic inflammation in mammals, constitutive activation of the gut innate immune response is detrimental to the health of flies. Accordingly, the Drosophila gut innate immune response is tightly regulated to maintain the endogenous microbiota, while preventing infections by pathogenic microorganisms.},
keywords = {fly, gut, innate immunity, M3i, matt, reichhart},
pubstate = {published},
tppubtype = {article}
}
Aguiar Eric Roberto Guimarães Rocha, Olmo Roenick Proveti, Paro Simona, Ferreira Flavia Viana, da de Faria Isaque João Silva, Todjro Yaovi Mathias Honore, Lobo Francisco Pereira, Kroon Erna Geessien, Meignin Carine, Gatherer Derek, Imler Jean-Luc, Marques João Trindade
Sequence-independent characterization of viruses based on the pattern of viral small RNAs produced by the host Article de journal
Dans: Nucleic Acids Research, vol. 43, no. 13, p. 6191–6206, 2015, ISSN: 1362-4962.
Résumé | Liens | BibTeX | Étiquettes: Animals, Contig Mapping, Female, imler, insects, M3i, meignin, Ovary, Plants, RNA, Sequence Analysis, Small Untranslated, Vertebrates, Viral, Viral Tropism, viruses
@article{aguiar_sequence-independent_2015,
title = {Sequence-independent characterization of viruses based on the pattern of viral small RNAs produced by the host},
author = {Eric Roberto Guimarães Rocha Aguiar and Roenick Proveti Olmo and Simona Paro and Flavia Viana Ferreira and Isaque João Silva da de Faria and Yaovi Mathias Honore Todjro and Francisco Pereira Lobo and Erna Geessien Kroon and Carine Meignin and Derek Gatherer and Jean-Luc Imler and João Trindade Marques},
url = {http://nar.oxfordjournals.org/lookup/doi/10.1093/nar/gkv587},
doi = {10.1093/nar/gkv587},
issn = {1362-4962},
year = {2015},
date = {2015-07-01},
journal = {Nucleic Acids Research},
volume = {43},
number = {13},
pages = {6191--6206},
abstract = {Virus surveillance in vector insects is potentially of great benefit to public health. Large-scale sequencing of small and long RNAs has previously been used to detect viruses, but without any formal comparison of different strategies. Furthermore, the identification of viral sequences largely depends on similarity searches against reference databases. Here, we developed a sequence-independent strategy based on virus-derived small RNAs produced by the host response, such as the RNA interference pathway. In insects, we compared sequences of small and long RNAs, demonstrating that viral sequences are enriched in the small RNA fraction. We also noted that the small RNA size profile is a unique signature for each virus and can be used to identify novel viral sequences without known relatives in reference databases. Using this strategy, we characterized six novel viruses in the viromes of laboratory fruit flies and wild populations of two insect vectors: mosquitoes and sandflies. We also show that the small RNA profile could be used to infer viral tropism for ovaries among other aspects of virus biology. Additionally, our results suggest that virus detection utilizing small RNAs can also be applied to vertebrates, although not as efficiently as to plants and insects.},
keywords = {Animals, Contig Mapping, Female, imler, insects, M3i, meignin, Ovary, Plants, RNA, Sequence Analysis, Small Untranslated, Vertebrates, Viral, Viral Tropism, viruses},
pubstate = {published},
tppubtype = {article}
}
Majzoub Karim, Imler Jean-Luc
Encyclopedia of Molecular Cell Biology and Molecular Medicine Chapitre d'ouvrage
Dans: Verlag, Wiley-VCH (Ed.): vol. 1, Chapitre « RNAi to treat virus infections », p. 192-228, GmbH & Co. KGaA, 2015.
Résumé | Liens | BibTeX | Étiquettes: antiviral, Argonaute, Delivery, imler, Immunity, lipofection, M3i, microRNA (miRNA), RNA Virus Infections, RNAi, small hairpin RNA (shRNA), small interfering RNA (siRNA)
@inbook{Majzoub2015,
title = {Encyclopedia of Molecular Cell Biology and Molecular Medicine},
author = {Karim Majzoub and Jean-Luc Imler},
editor = {Wiley-VCH Verlag},
doi = {10.1002/3527600906.mcb.201500003},
year = {2015},
date = {2015-04-28},
volume = {1},
pages = {192-228},
publisher = {GmbH & Co. KGaA},
chapter = {« RNAi to treat virus infections »},
abstract = {In spite of its young age, the field of RNA interference has already yielded major advances in the laboratory. This sequence-specific mechanism of gene regulation also holds strong promise for the development of a new generation of drugs, in particular to control the everlasting threat of viral infections. Here, the mechanisms and pathways of RNA interference are reviewed, with emphasis placed on how RNA silencing forms a potent antiviral immune mechanism in plants and invertebrates. The approaches developed to use RNA interference to control viral infections in mammals are then described. Finally, the problems encountered while translating this revolutionary technology into the clinic are presented, and the advances currently developed to overcome these limitations are discussed.},
keywords = {antiviral, Argonaute, Delivery, imler, Immunity, lipofection, M3i, microRNA (miRNA), RNA Virus Infections, RNAi, small hairpin RNA (shRNA), small interfering RNA (siRNA)},
pubstate = {published},
tppubtype = {inbook}
}
Volohonsky Gloria, Terenzi Olivier, Soichot Julien, Naujoks Daniel A, Nolan Tony, Windbichler Nikolai, Kapps Delphine, Smidler Andie L, Vittu Anaïs, Costa Giulia, Steinert Stefanie, Levashina Elena A, Blandin Stéphanie A, Marois Eric
Tools for Anopheles gambiae Transgenesis Article de journal
Dans: G3 (Bethesda), vol. 5, no. 6, p. 1151-63, 2015.
Résumé | Liens | BibTeX | Étiquettes: Anopheles gambiae, bioinformatic, blandin, M3i, marois, transgenesis
@article{G2015,
title = {Tools for Anopheles gambiae Transgenesis},
author = {Gloria Volohonsky and Olivier Terenzi and Julien Soichot and Daniel A Naujoks and Tony Nolan and Nikolai Windbichler and Delphine Kapps and Andie L Smidler and Anaïs Vittu and Giulia Costa and Stefanie Steinert and Elena A Levashina and Stéphanie A Blandin and Eric Marois},
url = {http://www.ncbi.nlm.nih.gov/pubmed/25869647},
year = {2015},
date = {2015-04-13},
journal = {G3 (Bethesda)},
volume = {5},
number = {6},
pages = {1151-63},
abstract = {Transgenesis is an essential tool to investigate gene function and to introduce desired characters in laboratory organisms. Setting-up transgenesis in non-model organisms is challenging due to the diversity of biological life traits and due to knowledge gaps in genomic information. Some procedures will be broadly applicable to many organisms, and others have to be specifically developed for the target species. Transgenesis in disease vector mosquitoes has existed since the 2000s but has remained limited by the delicate biology of these insects. Here, we report a compilation of the transgenesis tools that we have designed for the malaria vector Anopheles gambiae, including new docking strains, convenient transgenesis plasmids, a puromycin resistance selection marker, mosquitoes expressing cre recombinase, and various reporter lines defining the activity of cloned promoters. This toolbox contributed to rendering transgenesis routine in this species and is now enabling the development of increasingly refined genetic manipulations such as targeted mutagenesis. Some of the reagents and procedures reported here are easily transferable to other nonmodel species, including other disease vector or agricultural pest insects.},
keywords = {Anopheles gambiae, bioinformatic, blandin, M3i, marois, transgenesis},
pubstate = {published},
tppubtype = {article}
}
Paro Simona, Imler Jean-Luc, Meignin Carine
Sensing viral RNAs by Dicer/RIG-I like ATPases across species Article de journal
Dans: Current Opinion in Immunology, vol. 32, p. 106–113, 2015, ISSN: 1879-0372.
Résumé | Liens | BibTeX | Étiquettes: Adenosine Triphosphatases, Animals, DEAD-box RNA Helicases, Humans, imler, M3i, meignin, Protein Binding, Protein Interaction Domains and Motifs, Ribonuclease III, RNA, Viral, Virus Diseases, viruses
@article{paro_sensing_2015,
title = {Sensing viral RNAs by Dicer/RIG-I like ATPases across species},
author = {Simona Paro and Jean-Luc Imler and Carine Meignin},
url = {http://linkinghub.elsevier.com/retrieve/pii/S0952791515000102},
doi = {10.1016/j.coi.2015.01.009},
issn = {1879-0372},
year = {2015},
date = {2015-02-01},
journal = {Current Opinion in Immunology},
volume = {32},
pages = {106--113},
abstract = {Induction of antiviral immunity in vertebrates and invertebrates relies on members of the RIG-I-like receptor and Dicer families, respectively. Although these proteins have different size and domain composition, members of both families share a conserved DECH-box helicase domain. This helicase, also known as a duplex RNA activated ATPase, or DRA domain, plays an important role in viral RNA sensing. Crystallographic and electron microscopy studies of the RIG-I and Dicer DRA domains indicate a common structure and that similar conformational changes are induced by dsRNA binding. Genetic and biochemical studies on the function and regulation of DRAs reveal similarities, but also some differences, between viral RNA sensing mechanisms in nematodes, flies and mammals.},
keywords = {Adenosine Triphosphatases, Animals, DEAD-box RNA Helicases, Humans, imler, M3i, meignin, Protein Binding, Protein Interaction Domains and Motifs, Ribonuclease III, RNA, Viral, Virus Diseases, viruses},
pubstate = {published},
tppubtype = {article}
}
Neafsey D E, Waterhouse R M, Abai M R, Aganezov S S, Alekseyev M A, Allen J E, Amon J, Arcà B, Arensburger P, Artemov G, Assour L A, Basseri H, Berlin A, Birren B W, Blandin Stéphanie A, Brockman A I, Burkot T R, Burt A, Chan C S, Chauve C, Chiu J C, Christensen M, Costantini C, Davidson V L M, Deligianni E, Dottorini T, Dritsou V, Gabriel S B, Guelbeogo W M, Hall A B, Han M W, Hlaing T, Hughes D S T, Jenkins A M, Jiang X, Jungreis I, Kakani E G, Kamali M, Kemppainen P, Kennedy R C, Kirmitzoglou I K, Koekemoer L L, Laban N, Langridge N, Lawniczak M K N, Lirakis M, Lobo N F, Lowy E, MacCallum R M, Mao C, Maslen G, Mbogo C, McCarthy J, Michel K, Mitchell S N, Moore W, Murphy K A, Naumenko A N, Nolan Tony, Novoa E M, O’Loughlin S, Oringanje C, Oshaghi M A, Pakpour N, Papathanos P A, Peery A N, Povelones Michael, Prakash A, Price D A, Rajaraman A, Reimer L J, Rinker D C, Rokas A, Russell T L, Sagnon N F, Sharakhova M V, Shea T, Simão F A, Simard F, Slotman M A, Somboon P, Stegniy V, Struchiner C J, Thomas G W C, Tojo M, Topalis P, Tubio J M C, Unger M F, Vontas J, Walton C, Wilding C S, Willis J H, Wu Y-C, Yan G, Zdobnov E M, Zhou X, Catteruccia Flaminia, Christophides Georges K, Collins F H, Cornman R S, Crisanti Andrea, Donnelly M J, Emrich S J, Fontaine M C, Gelbart W, Hahn M W, Hansen I A, Howell P I, Kafatos Fotis C, Kellis M, Lawson D, Louis C, Luckhart S, Muskavitch M A T, Ribeiro J M, Riehle M A, Sharakhov I V, Tu Z, Zwiebel L J, Besansky N J
Highly evolvable malaria vectors: The genomes of 16 Anopheles mosquitoes. Article de journal
Dans: Science, vol. 347, no. 6217, p. 1258522, 2015.
Résumé | Liens | BibTeX | Étiquettes: Anopheles, blandin, genomes, M3i
@article{DE2015,
title = {Highly evolvable malaria vectors: The genomes of 16 Anopheles mosquitoes.},
author = {D E Neafsey and R M Waterhouse and M R Abai and S S Aganezov and M A Alekseyev and J E Allen and J Amon and B Arcà and P Arensburger and G Artemov and L A Assour and H Basseri and A Berlin and B W Birren and Stéphanie A Blandin and A I Brockman and T R Burkot and A Burt and C S Chan and C Chauve and J C Chiu and M Christensen and C Costantini and V L M Davidson and E Deligianni and T Dottorini and V Dritsou and S B Gabriel and W M Guelbeogo and A B Hall and M W Han and T Hlaing and D S T Hughes and A M Jenkins and X Jiang and I Jungreis and E G Kakani and M Kamali and P Kemppainen and R C Kennedy and I K Kirmitzoglou and L L Koekemoer and N Laban and N Langridge and M K N Lawniczak and M Lirakis and N F Lobo and E Lowy and R M MacCallum and C Mao and G Maslen and C Mbogo and J McCarthy and K Michel and S N Mitchell and W Moore and K A Murphy and A N Naumenko and Tony Nolan and E M Novoa and S O’Loughlin and C Oringanje and M A Oshaghi and N Pakpour and P A Papathanos and A N Peery and Michael Povelones and A Prakash and D A Price and A Rajaraman and L J Reimer and D C Rinker and A Rokas and T L Russell and N F Sagnon and M V Sharakhova and T Shea and F A Simão and F Simard and M A Slotman and P Somboon and V Stegniy and C J Struchiner and G W C Thomas and M Tojo and P Topalis and J M C Tubio and M F Unger and J Vontas and C Walton and C S Wilding and J H Willis and Y-C Wu and G Yan and E M Zdobnov and X Zhou and Flaminia Catteruccia and Georges K Christophides and F H Collins and R S Cornman and Andrea Crisanti and M J Donnelly and S J Emrich and M C Fontaine and W Gelbart and M W Hahn and I A Hansen and P I Howell and Fotis C Kafatos and M Kellis and D Lawson and C Louis and S Luckhart and M A T Muskavitch and J M Ribeiro and M A Riehle and I V Sharakhov and Z Tu and L J Zwiebel and N J Besansky},
url = {http://www.ncbi.nlm.nih.gov/pubmed/25554792},
year = {2015},
date = {2015-01-02},
journal = {Science},
volume = {347},
number = {6217},
pages = {1258522},
abstract = {Variation in vectorial capacity for human malaria among Anopheles mosquito species is determined by many factors, including behavior, immunity, and life history. To investigate the genomic basis of vectorial capacity and explore new avenues for vector control, we sequenced the genomes of 16 anopheline mosquito species from diverse locations spanning ~100 million years of evolution. Comparative analyses show faster rates of gene gain and loss, elevated gene shuffling on the X chromosome, and more intron losses, relative to Drosophila. Some determinants of vectorial capacity, such as chemosensory genes, do not show elevated turnover but instead diversify through protein-sequence changes. This dynamism of anopheline genes and genomes may contribute to their flexible capacity to take advantage of new ecological niches, including adapting to humans as primary hosts.},
keywords = {Anopheles, blandin, genomes, M3i},
pubstate = {published},
tppubtype = {article}
}
Hammond Andrew, Galizi Roberto, Kyrou Kyros, Simoni Alekos, Siniscalchi Carla, Katsanos Dimitris, Gribble Matthew, Baker Dean, Marois Eric, Russell Steven, Burt Austin, Windbichler Nikolai, Crisanti Andrea, Nolan Tony
A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae Article de journal
Dans: Nature Biotechnology, vol. 34, no. 1, p. 78–83, 2015, ISSN: 1087-0156, 1546-1696.
Liens | BibTeX | Étiquettes: M3i, marois
@article{hammond_crispr-cas9_2015,
title = {A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae},
author = {Andrew Hammond and Roberto Galizi and Kyros Kyrou and Alekos Simoni and Carla Siniscalchi and Dimitris Katsanos and Matthew Gribble and Dean Baker and Eric Marois and Steven Russell and Austin Burt and Nikolai Windbichler and Andrea Crisanti and Tony Nolan},
url = {http://www.nature.com/doifinder/10.1038/nbt.3439},
doi = {10.1038/nbt.3439},
issn = {1087-0156, 1546-1696},
year = {2015},
date = {2015-01-01},
urldate = {2016-01-26},
journal = {Nature Biotechnology},
volume = {34},
number = {1},
pages = {78--83},
keywords = {M3i, marois},
pubstate = {published},
tppubtype = {article}
}
Brunke Sascha, Quintin Jessica, Kasper Lydia, Jacobsen Ilse D, Richter Martin E, Hiller Ekkehard, Schwarzmüller Tobias, d'Enfert Christophe, Kuchler Karl, Rupp Steffen, Hube Bernhard, Ferrandon Dominique
Of mice, flies--and men? Comparing fungal infection models for large-scale screening efforts Article de journal
Dans: Dis Model Mech, vol. 8, no. 5, p. 473–486, 2015, ISSN: 1754-8411.
Résumé | Liens | BibTeX | Étiquettes: Alternative infection models, Candida glabrata, ferrandon, Fungal virulence factors, M3i, Mutant library, Signature-tagged mutagenesis
@article{brunke_mice_2015b,
title = {Of mice, flies--and men? Comparing fungal infection models for large-scale screening efforts},
author = {Sascha Brunke and Jessica Quintin and Lydia Kasper and Ilse D Jacobsen and Martin E Richter and Ekkehard Hiller and Tobias Schwarzmüller and Christophe d'Enfert and Karl Kuchler and Steffen Rupp and Bernhard Hube and Dominique Ferrandon},
url = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4415897/},
doi = {10.1242/dmm.019901},
issn = {1754-8411},
year = {2015},
date = {2015-01-01},
journal = {Dis Model Mech},
volume = {8},
number = {5},
pages = {473--486},
abstract = {Studying infectious diseases requires suitable hosts for experimental in vivo infections. Recent years have seen the advent of many alternatives to murine infection models. However, the use of non-mammalian models is still controversial because it is often unclear how well findings from these systems predict virulence potential in humans or other mammals. Here, we compare the commonly used models, fruit fly and mouse (representing invertebrate and mammalian hosts), for their similarities and degree of correlation upon infection with a library of mutants of an important fungal pathogen, the yeast Candida glabrata. Using two indices, for fly survival time and for mouse fungal burden in specific organs, we show a good agreement between the models. We provide a suitable predictive model for estimating the virulence potential of C. glabrata mutants in the mouse from fly survival data. As examples, we found cell wall integrity mutants attenuated in flies, and mutants of a MAP kinase pathway had defective virulence in flies and reduced relative pathogen fitness in mice. In addition, mutants with strongly reduced in vitro growth generally, but not always, had reduced virulence in flies. Overall, we demonstrate that surveying Drosophila survival after infection is a suitable model to predict the outcome of murine infections, especially for severely attenuated C. glabrata mutants. Pre-screening of mutants in an invertebrate Drosophila model can, thus, provide a good estimate of the probability of finding a strain with reduced microbial burden in the mouse host.},
keywords = {Alternative infection models, Candida glabrata, ferrandon, Fungal virulence factors, M3i, Mutant library, Signature-tagged mutagenesis},
pubstate = {published},
tppubtype = {article}
}
2014
Chtarbanova Stanislava, Lamiable Olivier, Lee Kwang-Zin, Galiana Delphine, Troxler Laurent, Meignin Carine, Hetru Charles, Hoffmann Jules A, Daeffler Laurent, Imler Jean-Luc
Drosophila C virus systemic infection leads to intestinal obstruction Article de journal
Dans: Journal of Virology, vol. 88, no. 24, p. 14057–14069, 2014, ISSN: 1098-5514.
Résumé | Liens | BibTeX | Étiquettes: Animals, bioinformatic, Dicistroviridae, Female, Gastrointestinal Tract, Gene Expression Profiling, hoffmann, imler, Intestinal Obstruction, M3i, meignin, Muscle, Nodaviridae, Sindbis Virus, Smooth, Viral Tropism
@article{chtarbanova_drosophila_2014,
title = {Drosophila C virus systemic infection leads to intestinal obstruction},
author = {Stanislava Chtarbanova and Olivier Lamiable and Kwang-Zin Lee and Delphine Galiana and Laurent Troxler and Carine Meignin and Charles Hetru and Jules A Hoffmann and Laurent Daeffler and Jean-Luc Imler},
url = {http://jvi.asm.org/content/88/24/14057},
doi = {10.1128/JVI.02320-14},
issn = {1098-5514},
year = {2014},
date = {2014-12-01},
journal = {Journal of Virology},
volume = {88},
number = {24},
pages = {14057--14069},
abstract = {Drosophila C virus (DCV) is a positive-sense RNA virus belonging to the Dicistroviridae family. This natural pathogen of the model organism Drosophila melanogaster is commonly used to investigate antiviral host defense in flies, which involves both RNA interference and inducible responses. Although lethality is used routinely as a readout for the efficiency of the antiviral immune response in these studies, virus-induced pathologies in flies still are poorly understood. Here, we characterize the pathogenesis associated with systemic DCV infection. Comparison of the transcriptome of flies infected with DCV or two other positive-sense RNA viruses, Flock House virus and Sindbis virus, reveals that DCV infection, unlike those of the other two viruses, represses the expression of a large number of genes. Several of these genes are expressed specifically in the midgut and also are repressed by starvation. We show that systemic DCV infection triggers a nutritional stress in Drosophila which results from intestinal obstruction with the accumulation of peritrophic matrix at the entry of the midgut and the accumulation of the food ingested in the crop, a blind muscular food storage organ. The related virus cricket paralysis virus (CrPV), which efficiently grows in Drosophila, does not trigger this pathology. We show that DCV, but not CrPV, infects the smooth muscles surrounding the crop, causing extensive cytopathology and strongly reducing the rate of contractions. We conclude that the pathogenesis associated with systemic DCV infection results from the tropism of the virus for an important organ within the foregut of dipteran insects, the crop. IMPORTANCE: DCV is one of the few identified natural viral pathogens affecting the model organism Drosophila melanogaster. As such, it is an important virus for the deciphering of host-virus interactions in insects. We characterize here the pathogenesis associated with DCV infection in flies and show that it results from the tropism of the virus for an essential but poorly characterized organ in the digestive tract, the crop. Our results may have relevance for other members of the Dicistroviridae, some of which are pathogenic to beneficial or pest insect species.},
keywords = {Animals, bioinformatic, Dicistroviridae, Female, Gastrointestinal Tract, Gene Expression Profiling, hoffmann, imler, Intestinal Obstruction, M3i, meignin, Muscle, Nodaviridae, Sindbis Virus, Smooth, Viral Tropism},
pubstate = {published},
tppubtype = {article}
}
Gabrieli P, Marois Eric, Catteruccia Flaminia
Transgenic insects: techniques and applications Chapitre d'ouvrage
Dans: Benedicts, MQ (Ed.): Chapitre Sexual sterilization of mosquitoes, p. 188-207, CABI, 2014.
BibTeX | Étiquettes: insects, M3i, marois, sterile
@inbook{P2014,
title = {Transgenic insects: techniques and applications},
author = {P Gabrieli and Eric Marois and Flaminia Catteruccia},
editor = {MQ Benedicts},
year = {2014},
date = {2014-10-01},
pages = {188-207},
publisher = {CABI},
chapter = {Sexual sterilization of mosquitoes},
keywords = {insects, M3i, marois, sterile},
pubstate = {published},
tppubtype = {inbook}
}
Bonnay François, Nguyen Xuan-Hung, Cohen-Berros Eva, Troxler Laurent, Batsche Eric, Camonis Jacques, Takeuchi Osamu, Reichhart Jean-Marc, Matt Nicolas
Akirin specifies NF-κB selectivity of Drosophila innate immune response via chromatin remodeling Article de journal
Dans: EMBO J., vol. 33, no. 20, p. 2349–2362, 2014, ISSN: 1460-2075.
Résumé | Liens | BibTeX | Étiquettes: Animals, bioinformatic, Cell Cycle Proteins, Chromatin Assembly and Disassembly, chromatin remodeling, DNA-Binding Proteins, Female, Genetic, Immunity, Innate, Innate immune response, M3i, Male, matt, Mutation, NF-kappa B, NF‐κB, Promoter Regions, proteomics, reichhart, Trans-Activators, Transcription Factors, Transcriptional Activation, Two-Hybrid System Techniques
@article{bonnay_akirin_2014,
title = {Akirin specifies NF-κB selectivity of Drosophila innate immune response via chromatin remodeling},
author = {François Bonnay and Xuan-Hung Nguyen and Eva Cohen-Berros and Laurent Troxler and Eric Batsche and Jacques Camonis and Osamu Takeuchi and Jean-Marc Reichhart and Nicolas Matt},
doi = {10.15252/embj.201488456},
issn = {1460-2075},
year = {2014},
date = {2014-10-01},
journal = {EMBO J.},
volume = {33},
number = {20},
pages = {2349--2362},
abstract = {The network of NF-κB-dependent transcription that activates both pro- and anti-inflammatory genes in mammals is still unclear. As NF-κB factors are evolutionarily conserved, we used Drosophila to understand this network. The NF-κB transcription factor Relish activates effector gene expression following Gram-negative bacterial immune challenge. Here, we show, using a genome-wide approach, that the conserved nuclear protein Akirin is a NF-κB co-factor required for the activation of a subset of Relish-dependent genes correlating with the presence of H3K4ac epigenetic marks. A large-scale unbiased proteomic analysis revealed that Akirin orchestrates NF-κB transcriptional selectivity through the recruitment of the Osa-containing-SWI/SNF-like Brahma complex (BAP). Immune challenge in Drosophila shows that Akirin is required for the transcription of a subset of effector genes, but dispensable for the transcription of genes that are negative regulators of the innate immune response. Therefore, Akirins act as molecular selectors specifying the choice between subsets of NF-κB target genes. The discovery of this mechanism, conserved in mammals, paves the way for the establishment of more specific and less toxic anti-inflammatory drugs targeting pro-inflammatory genes.},
keywords = {Animals, bioinformatic, Cell Cycle Proteins, Chromatin Assembly and Disassembly, chromatin remodeling, DNA-Binding Proteins, Female, Genetic, Immunity, Innate, Innate immune response, M3i, Male, matt, Mutation, NF-kappa B, NF‐κB, Promoter Regions, proteomics, reichhart, Trans-Activators, Transcription Factors, Transcriptional Activation, Two-Hybrid System Techniques},
pubstate = {published},
tppubtype = {article}
}
Tartey Sarang, Matsushita Kazufumi, Vandenbon Alexis, Ori Daisuke, Imamura Tomoko, Mino Takashi, Standley Daron M, Hoffmann Jules A, Reichhart Jean-Marc, Akira Shizuo, Takeuchi Osamu
Akirin2 is critical for inducing inflammatory genes by bridging IκB-ζ and the SWI/SNF complex Article de journal
Dans: EMBO J., vol. 33, no. 20, p. 2332–2348, 2014, ISSN: 1460-2075.
Résumé | Liens | BibTeX | Étiquettes: Adaptor Proteins, Animals, Cell Nucleus, Chromatin Assembly and Disassembly, chromatin remodeling, Chromosomal Proteins, cytokine, Cytokines, Female, Gene Expression Regulation, gene regulation, Genetic, hoffmann, Humans, Immunity, Innate, innate immunity, Knockout, Listeria monocytogenes, M3i, Macrophages, Male, Mice, Multiprotein Complexes, Non-Histone, Nuclear Proteins, Promoter Regions, Protein Binding, reichhart, Repressor Proteins, Sequence Deletion, Signal Transducing, Transcriptional Activation
@article{tartey_akirin2_2014,
title = {Akirin2 is critical for inducing inflammatory genes by bridging IκB-ζ and the SWI/SNF complex},
author = {Sarang Tartey and Kazufumi Matsushita and Alexis Vandenbon and Daisuke Ori and Tomoko Imamura and Takashi Mino and Daron M Standley and Jules A Hoffmann and Jean-Marc Reichhart and Shizuo Akira and Osamu Takeuchi},
doi = {10.15252/embj.201488447},
issn = {1460-2075},
year = {2014},
date = {2014-10-01},
journal = {EMBO J.},
volume = {33},
number = {20},
pages = {2332--2348},
abstract = {Transcription of inflammatory genes in innate immune cells is coordinately regulated by transcription factors, including NF-κB, and chromatin modifiers. However, it remains unclear how microbial sensing initiates chromatin remodeling. Here, we show that Akirin2, an evolutionarily conserved nuclear protein, bridges NF-κB and the chromatin remodeling SWI/SNF complex by interacting with BRG1-Associated Factor 60 (BAF60) proteins as well as IκB-ζ, which forms a complex with the NF-κB p50 subunit. These interactions are essential for Toll-like receptor-, RIG-I-, and Listeria-mediated expression of proinflammatory genes including Il6 and Il12b in macrophages. Consistently, effective clearance of Listeria infection required Akirin2. Furthermore, Akirin2 and IκB-ζ recruitment to the Il6 promoter depend upon the presence of IκB-ζ and Akirin2, respectively, for regulation of chromatin remodeling. BAF60 proteins were also essential for the induction of Il6 in response to LPS stimulation. Collectively, the IκB-ζ-Akirin2-BAF60 complex physically links the NF-κB and SWI/SNF complexes in innate immune cell activation. By recruiting SWI/SNF chromatin remodellers to IκB-ζ, transcriptional coactivator for NF-κB, the conserved nuclear protein Akirin2 stimulates pro-inflammatory gene promoters in mouse macrophages during innate immune responses to viral or bacterial infection.},
keywords = {Adaptor Proteins, Animals, Cell Nucleus, Chromatin Assembly and Disassembly, chromatin remodeling, Chromosomal Proteins, cytokine, Cytokines, Female, Gene Expression Regulation, gene regulation, Genetic, hoffmann, Humans, Immunity, Innate, innate immunity, Knockout, Listeria monocytogenes, M3i, Macrophages, Male, Mice, Multiprotein Complexes, Non-Histone, Nuclear Proteins, Promoter Regions, Protein Binding, reichhart, Repressor Proteins, Sequence Deletion, Signal Transducing, Transcriptional Activation},
pubstate = {published},
tppubtype = {article}
}
Amcheslavsky Alla, Song Wei, Li Qi, Nie Yingchao, Bragatto Ivan, Ferrandon Dominique, Perrimon Norbert, Ip Tony Y
Enteroendocrine cells support intestinal stem-cell-mediated homeostasis in Drosophila Article de journal
Dans: Cell Rep, vol. 9, no. 1, p. 32–39, 2014, ISSN: 2211-1247.
Résumé | Liens | BibTeX | Étiquettes: Animals, Cell Differentiation, Enterocytes, Enteroendocrine Cells, Female, ferrandon, Homeostasis, Intestines, M3i, Male, Stem Cells, Tachykinins
@article{amcheslavsky_enteroendocrine_2014b,
title = {Enteroendocrine cells support intestinal stem-cell-mediated homeostasis in Drosophila},
author = {Alla Amcheslavsky and Wei Song and Qi Li and Yingchao Nie and Ivan Bragatto and Dominique Ferrandon and Norbert Perrimon and Tony Y Ip},
doi = {10.1016/j.celrep.2014.08.052},
issn = {2211-1247},
year = {2014},
date = {2014-10-01},
journal = {Cell Rep},
volume = {9},
number = {1},
pages = {32--39},
abstract = {Intestinal stem cells in the adult Drosophila midgut are regulated by growth factors produced from the surrounding niche cells including enterocytes and visceral muscle. The role of the other major cell type, the secretory enteroendocrine cells, in regulating intestinal stem cells remains unclear. We show here that newly eclosed scute loss-of-function mutant flies are completely devoid of enteroendocrine cells. These enteroendocrine cell-less flies have normal ingestion and fecundity but shorter lifespan. Moreover, in these newly eclosed mutant flies, the diet-stimulated midgut growth that depends on the insulin-like peptide 3 expression in the surrounding muscle is defective. The depletion of Tachykinin-producing enteroendocrine cells or knockdown of Tachykinin leads to a similar although less severe phenotype. These results establish that enteroendocrine cells serve as an important link between diet and visceral muscle expression of an insulin-like growth factor to stimulate intestinal stem cell proliferation and tissue growth.},
keywords = {Animals, Cell Differentiation, Enterocytes, Enteroendocrine Cells, Female, ferrandon, Homeostasis, Intestines, M3i, Male, Stem Cells, Tachykinins},
pubstate = {published},
tppubtype = {article}
}
Lamiable Olivier, Imler Jean-Luc
Induced antiviral innate immunity in Drosophila Article de journal
Dans: Current Opinion in Microbiology, vol. 20, p. 62–68, 2014, ISSN: 1879-0364.
Résumé | Liens | BibTeX | Étiquettes: Animals, Gene Expression Regulation, Host-Pathogen Interactions, imler, Immunity, Innate, M3i, RNA Viruses, Signal Transduction
@article{lamiable_induced_2014,
title = {Induced antiviral innate immunity in Drosophila},
author = {Olivier Lamiable and Jean-Luc Imler},
doi = {10.1016/j.mib.2014.05.006},
issn = {1879-0364},
year = {2014},
date = {2014-08-01},
journal = {Current Opinion in Microbiology},
volume = {20},
pages = {62--68},
abstract = {Immunity to viral infections in the model organism Drosophila melanogaster involves both RNA interference and additional induced responses. The latter include not only cellular mechanisms such as programmed cell death and autophagy, but also the induction of a large set of genes, some of which contribute to the control of viral replication and resistance to infection. This induced response to infection is complex and involves both virus-specific and cell-type specific mechanisms. We review here recent developments, from the sensing of viral infection to the induction of signaling pathways and production of antiviral effector molecules. Our current understanding, although still partial, validates the Drosophila model of antiviral induced immunity for insect pests and disease vectors, as well as for mammals.},
keywords = {Animals, Gene Expression Regulation, Host-Pathogen Interactions, imler, Immunity, Innate, M3i, RNA Viruses, Signal Transduction},
pubstate = {published},
tppubtype = {article}
}
Goto Akira, Fukuyama Hidehiro, Imler Jean-Luc, Hoffmann Jules A
The chromatin regulator DMAP1 modulates activity of the nuclear factor B (NF-B) transcription factor Relish in the Drosophila innate immune response Article de journal
Dans: The Journal of Biological Chemistry, vol. 289, no. 30, p. 20470–20476, 2014, ISSN: 1083-351X.
Résumé | Liens | BibTeX | Étiquettes: Animals, Cell Line, Chromatin Assembly and Disassembly, Epistasis, Escherichia coli, Escherichia coli Infections, Genetic, hoffmann, imler, Immunity, Innate, M3i, NF-kappa B, Repressor Proteins, Signal Transduction, Transcription Factors
@article{goto_chromatin_2014,
title = {The chromatin regulator DMAP1 modulates activity of the nuclear factor B (NF-B) transcription factor Relish in the Drosophila innate immune response},
author = {Akira Goto and Hidehiro Fukuyama and Jean-Luc Imler and Jules A Hoffmann},
doi = {10.1074/jbc.C114.553719},
issn = {1083-351X},
year = {2014},
date = {2014-07-01},
journal = {The Journal of Biological Chemistry},
volume = {289},
number = {30},
pages = {20470--20476},
abstract = {The host defense of the model organism Drosophila is under the control of two major signaling cascades controlling transcription factors of the NF-B family, the Toll and the immune deficiency (IMD) pathways. The latter shares extensive similarities with the mammalian TNF-R pathway and was initially discovered for its role in anti-Gram-negative bacterial reactions. A previous interactome study from this laboratory reported that an unexpectedly large number of proteins are binding to the canonical components of the IMD pathway. Here, we focus on DNA methyltransferase-associated protein 1 (DMAP1), which this study identified as an interactant of Relish, a Drosophila transcription factor reminiscent of the mammalian p105 NF-B protein. We show that silencing of DMAP1 expression both in S2 cells and in flies results in a significant reduction of Escherichia coli-induced expression of antimicrobial peptides. Epistatic analysis indicates that DMAP1 acts in parallel or downstream of Relish. Co-immunoprecipitation experiments further reveal that, in addition to Relish, DMAP1 also interacts with Akirin and the Brahma-associated protein 55 kDa (BAP55). Taken together, these results reveal that DMAP1 is a novel nuclear modulator of the IMD pathway, possibly acting at the level of chromatin remodeling.},
keywords = {Animals, Cell Line, Chromatin Assembly and Disassembly, Epistasis, Escherichia coli, Escherichia coli Infections, Genetic, hoffmann, imler, Immunity, Innate, M3i, NF-kappa B, Repressor Proteins, Signal Transduction, Transcription Factors},
pubstate = {published},
tppubtype = {article}
}
Imler Jean-Luc, Hoffmann Jules A
Le défi des maladies infectieuses Chapitre d'ouvrage
Dans: collection Guérir et prévenir demain, Ph. Cramer (Ed.): Chapitre « L’immunité innée », p. 167-174, Editions DOCIS, 2014, ISBN: 978-2-85525-390-9.
Résumé | BibTeX | Étiquettes: hoffmann, imler, innate immunity, M3i, maladies infectieuses
@inbook{Imler2014,
title = {Le défi des maladies infectieuses},
author = {Jean-Luc Imler and Jules A Hoffmann},
editor = {Ph. Cramer collection Guérir et prévenir demain},
isbn = {978-2-85525-390-9},
year = {2014},
date = {2014-06-01},
pages = {167-174},
publisher = {Editions DOCIS},
chapter = {« L’immunité innée »},
abstract = {La lèpre fait des ravages dès l’Antiquité, les épidémies de peste tuent au Moyen Âge et celles de choléra dévastent l’Inde. La tuberculose émerge véritablement au XIXème siècle, la grippe espagnole a fait vingt millions de morts en 1918 et de nouvelles maladies apparaissent : les infections hospitalières, les hépatites, le SIDA, les fièvres hémorragiques, la légionellose,…
Toutes ces maladies ont un point commun, ce sont des maladies infectieuses qui sont pour la médecine un vrai défi, tant elles sont dévastatrices. L’innovation a eu et continue à avoir un rôle essentiel dans la caractérisation de ces maladies, la découverte de l’agent responsable, leur traitement et leur prévention. Ce livre, dont les auteurs font partie des plus éminents spécialistes français et européens des maladies infectieuses décrit, de façon abordable par tous, aussi bien les découvertes et les inventions essentielles à ce domaine que les avancées médicales.},
keywords = {hoffmann, imler, innate immunity, M3i, maladies infectieuses},
pubstate = {published},
tppubtype = {inbook}
}
Toutes ces maladies ont un point commun, ce sont des maladies infectieuses qui sont pour la médecine un vrai défi, tant elles sont dévastatrices. L’innovation a eu et continue à avoir un rôle essentiel dans la caractérisation de ces maladies, la découverte de l’agent responsable, leur traitement et leur prévention. Ce livre, dont les auteurs font partie des plus éminents spécialistes français et européens des maladies infectieuses décrit, de façon abordable par tous, aussi bien les découvertes et les inventions essentielles à ce domaine que les avancées médicales.
Bernardini F, Galizi R, Menichelli M, Papathanos P A, Dritsou V, Marois Eric, Crisanti Andrea, Windbichler Nikolai
Site-specific genetic engineering of the Anopheles gambiae Y chromosome Article de journal
Dans: Proc Natl Acad Sci U S A., vol. 111, no. 21, p. 7600-5, 2014.
Résumé | Liens | BibTeX | Étiquettes: Anopheles gambiae, Biotechnology, M3i, marois, SIT, transgenesis
@article{F2014b,
title = {Site-specific genetic engineering of the Anopheles gambiae Y chromosome},
author = {F Bernardini and R Galizi and M Menichelli and P A Papathanos and V Dritsou and Eric Marois and Andrea Crisanti and Nikolai Windbichler},
url = {http://www.ncbi.nlm.nih.gov/pubmed/24821795},
year = {2014},
date = {2014-05-27},
journal = {Proc Natl Acad Sci U S A.},
volume = {111},
number = {21},
pages = {7600-5},
abstract = {Despite its function in sex determination and its role in driving genome evolution, the Y chromosome remains poorly understood in most species. Y chromosomes are gene-poor, repeat-rich and largely heterochromatic and therefore represent a difficult target for genetic engineering. The Y chromosome of the human malaria vector Anopheles gambiae appears to be involved in sex determination although very little is known about both its structure and function. Here, we characterize a transgenic strain of this mosquito species, obtained by transposon-mediated integration of a transgene construct onto the Y chromosome. Using meganuclease-induced homologous repair we introduce a site-specific recombination signal onto the Y chromosome and show that the resulting docking line can be used for secondary integration. To demonstrate its utility, we study the activity of a germ-line-specific promoter when located on the Y chromosome. We also show that Y-linked fluorescent transgenes allow automated sex separation of this important vector species, providing the means to generate large single-sex populations. Our findings will aid studies of sex chromosome function and enable the development of male-exclusive genetic traits for vector control.},
keywords = {Anopheles gambiae, Biotechnology, M3i, marois, SIT, transgenesis},
pubstate = {published},
tppubtype = {article}
}
Imler Jean-Luc
Overview of Drosophila immunity: a historical perspective Article de journal
Dans: Developmental and Comparative Immunology, vol. 42, no. 1, p. 3–15, 2014, ISSN: 1879-0089.
Résumé | Liens | BibTeX | Étiquettes: Allergy and Immunology, Animal, Animals, Antimicrobial Cationic Peptides, Antimicrobial peptides, history, Humans, IMD pathway, imler, Immunity, Innate, innate immunity, M3i, Models, Pattern recognition receptors, Signal Transduction, Toll-Like Receptors
@article{imler_overview_2014,
title = {Overview of Drosophila immunity: a historical perspective},
author = {Jean-Luc Imler},
doi = {10.1016/j.dci.2013.08.018},
issn = {1879-0089},
year = {2014},
date = {2014-01-01},
journal = {Developmental and Comparative Immunology},
volume = {42},
number = {1},
pages = {3--15},
abstract = {The functional analysis of genes from the model organism Drosophila melanogaster has provided invaluable information for many cellular and developmental or physiological processes, including immunity. The best-understood aspect of Drosophila immunity is the inducible humoral response, first recognized in 1972. This pioneering work led to a remarkable series of findings over the next 30 years, ranging from the identification and characterization of the antimicrobial peptides produced, to the deciphering of the signalling pathways activating the genes that encode them and, ultimately, to the discovery of the receptors sensing infection. These studies on an insect model coincided with a revival of the field of innate immunity, and had an unanticipated impact on the biomedical field.},
keywords = {Allergy and Immunology, Animal, Animals, Antimicrobial Cationic Peptides, Antimicrobial peptides, history, Humans, IMD pathway, imler, Immunity, Innate, innate immunity, M3i, Models, Pattern recognition receptors, Signal Transduction, Toll-Like Receptors},
pubstate = {published},
tppubtype = {article}
}
Leclerc Vincent, Chamy Laure El, Ntwasa Monde, Reichhart Jean-Marc
Drosophila melanogaster model in innate immunity. Article de journal
Dans: Trends in Entomology, vol. 10, p. 73–86, 2014.
Résumé | BibTeX | Étiquettes: M3i, reichhart
@article{leclerc_drosophila_2014,
title = {Drosophila melanogaster model in innate immunity.},
author = {Vincent Leclerc and Laure El Chamy and Monde Ntwasa and Jean-Marc Reichhart},
year = {2014},
date = {2014-01-01},
journal = {Trends in Entomology},
volume = {10},
pages = {73--86},
abstract = {Due relative simplicity of the fly and availability of large amounts of genetic tools, Drosophila melanogaster has proven to be an excellent model to study the basic principles of innate immunity. This is illustrated by the discovery of the Toll-like receptor functions in pathogen sensing, recognised by the 2011 Nobel Prize in Physiology and Medicine awarded to Jules Hoffmann. Drosophila can also be used as an in vivo, genetically tractable model, to analyse various aspects of host-pathogen interactions including virulence factor mechanisms of action.},
keywords = {M3i, reichhart},
pubstate = {published},
tppubtype = {article}
}
Haller Samantha, Limmer Stefanie, Ferrandon Dominique
Assessing Pseudomonas virulence with a nonmammalian host: Drosophila melanogaster Article de journal
Dans: Methods Mol. Biol., vol. 1149, p. 723–740, 2014, ISSN: 1940-6029.
Résumé | Liens | BibTeX | Étiquettes: Animal, Animals, Antimicrobial Cationic Peptides, Biological Assay, Colony Count, Disease Models, ferrandon, Hemolymph, Host-Pathogen Interactions, M3i, Mammals, Microbial, Pseudomonas aeruginosa, Pseudomonas Infections, Reverse Transcriptase Polymerase Chain Reaction, Virulence
@article{haller_assessing_2014b,
title = {Assessing Pseudomonas virulence with a nonmammalian host: Drosophila melanogaster},
author = {Samantha Haller and Stefanie Limmer and Dominique Ferrandon},
doi = {10.1007/978-1-4939-0473-0_56},
issn = {1940-6029},
year = {2014},
date = {2014-01-01},
journal = {Methods Mol. Biol.},
volume = {1149},
pages = {723--740},
abstract = {Drosophila melanogaster flies represent an interesting model to study host-pathogen interactions as: (1) they are cheap and easy to raise rapidly and do not bring up ethical issues, (2) available genetic tools are highly sophisticated, for instance allowing tissue-specific alteration of gene expression, e.g., of immune genes, (3) they have a relatively complex organization, with distinct digestive tract and body cavity in which local or systemic infections, respectively, take place, (4) a medium throughput can be achieved in genetic screens, for instance looking for Pseudomonas aeruginosa mutants with altered virulence. We present here the techniques used to investigate host-pathogen relationships, namely the two major models of infections as well as the relevant parameters used to monitor the infection (survival, bacterial titer, induction of host immune response).},
keywords = {Animal, Animals, Antimicrobial Cationic Peptides, Biological Assay, Colony Count, Disease Models, ferrandon, Hemolymph, Host-Pathogen Interactions, M3i, Mammals, Microbial, Pseudomonas aeruginosa, Pseudomonas Infections, Reverse Transcriptase Polymerase Chain Reaction, Virulence},
pubstate = {published},
tppubtype = {article}
}
Lestradet Matthieu, Lee Kwan Zin, Ferrandon Dominique
Drosophila as a model for intestinal infections Article de journal
Dans: Methods Mol Biol, vol. 1197, p. 11–40, 2014, ISSN: 1940-6029 (Electronic) 1064-3745 (Linking).
Résumé | Liens | BibTeX | Étiquettes: Animal, Animals, Bacterial Physiological Phenomena, Disease Models, ferrandon, Gastrointestinal Tract, Host-Pathogen Interactions, M3i
@article{lestradet_drosophila_2014b,
title = {Drosophila as a model for intestinal infections},
author = {Matthieu Lestradet and Kwan Zin Lee and Dominique Ferrandon},
doi = {10.1007/978-1-4939-1261-2_2},
issn = {1940-6029 (Electronic) 1064-3745 (Linking)},
year = {2014},
date = {2014-01-01},
journal = {Methods Mol Biol},
volume = {1197},
pages = {11--40},
abstract = {Drosophila melanogaster is a powerful model to study infections thanks to the power of its genetics and knowledge on its biology accumulated for over a century. While the systemic humoral immune response against invading microbes has been intensively studied in the past two decades, the study of intestinal infections is more recent. Here, we present the methods that are currently in use to probe various aspects of the host-pathogen interactions between Drosophila and ingested microbes, with an emphasis on the study of the midgut epithelium, which constitutes the major interface between the organism and the microbe-rich ingested food.},
keywords = {Animal, Animals, Bacterial Physiological Phenomena, Disease Models, ferrandon, Gastrointestinal Tract, Host-Pathogen Interactions, M3i},
pubstate = {published},
tppubtype = {article}
}
Schwarzmüller Tobias, Ma Biao, Hiller Ekkehard, Istel Fabian, Tscherner Michael, Brunke Sascha, Ames Lauren, Firon Arnaud, Green Brian, Cabral Vitor, Marcet-Houben Marina, Jacobsen Ilse D, Quintin Jessica, Seider Katja, Frohner Ingrid, Glaser Walter, Jungwirth Helmut, Bachellier-Bassi Sophie, Chauvel Murielle, Zeidler Ute, Ferrandon Dominique, Gabaldón Toni, Hube Bernhard, d'Enfert Christophe, Rupp Steffen, Cormack Brendan, Haynes Ken, Kuchler Karl
Systematic phenotyping of a large-scale Candida glabrata deletion collection reveals novel antifungal tolerance genes Article de journal
Dans: PLoS Pathog., vol. 10, no. 6, p. e1004211, 2014, ISSN: 1553-7374.
Résumé | Liens | BibTeX | Étiquettes: Antifungal Agents, Azoles, Biofilms, Candida glabrata, Candidiasis, Cell Wall, Drug Resistance, Echinocandins, ferrandon, Fungal, Fungal Proteins, Gene Deletion, Gene Knockout Techniques, Gene Library, M3i, Microbial Sensitivity Tests, Osmotic Pressure, Phenotype
@article{schwarzmuller_systematic_2014b,
title = {Systematic phenotyping of a large-scale Candida glabrata deletion collection reveals novel antifungal tolerance genes},
author = {Tobias Schwarzmüller and Biao Ma and Ekkehard Hiller and Fabian Istel and Michael Tscherner and Sascha Brunke and Lauren Ames and Arnaud Firon and Brian Green and Vitor Cabral and Marina Marcet-Houben and Ilse D Jacobsen and Jessica Quintin and Katja Seider and Ingrid Frohner and Walter Glaser and Helmut Jungwirth and Sophie Bachellier-Bassi and Murielle Chauvel and Ute Zeidler and Dominique Ferrandon and Toni Gabaldón and Bernhard Hube and Christophe d'Enfert and Steffen Rupp and Brendan Cormack and Ken Haynes and Karl Kuchler},
doi = {10.1371/journal.ppat.1004211},
issn = {1553-7374},
year = {2014},
date = {2014-01-01},
journal = {PLoS Pathog.},
volume = {10},
number = {6},
pages = {e1004211},
abstract = {The opportunistic fungal pathogen Candida glabrata is a frequent cause of candidiasis, causing infections ranging from superficial to life-threatening disseminated disease. The inherent tolerance of C. glabrata to azole drugs makes this pathogen a serious clinical threat. To identify novel genes implicated in antifungal drug tolerance, we have constructed a large-scale C. glabrata deletion library consisting of 619 unique, individually bar-coded mutant strains, each lacking one specific gene, all together representing almost 12% of the genome. Functional analysis of this library in a series of phenotypic and fitness assays identified numerous genes required for growth of C. glabrata under normal or specific stress conditions, as well as a number of novel genes involved in tolerance to clinically important antifungal drugs such as azoles and echinocandins. We identified 38 deletion strains displaying strongly increased susceptibility to caspofungin, 28 of which encoding proteins that have not previously been linked to echinocandin tolerance. Our results demonstrate the potential of the C. glabrata mutant collection as a valuable resource in functional genomics studies of this important fungal pathogen of humans, and to facilitate the identification of putative novel antifungal drug target and virulence genes.},
keywords = {Antifungal Agents, Azoles, Biofilms, Candida glabrata, Candidiasis, Cell Wall, Drug Resistance, Echinocandins, ferrandon, Fungal, Fungal Proteins, Gene Deletion, Gene Knockout Techniques, Gene Library, M3i, Microbial Sensitivity Tests, Osmotic Pressure, Phenotype},
pubstate = {published},
tppubtype = {article}
}
Majzoub K, Hafirassou M L, Meignin C, Goto A, Marzi S, Fedorova A, Verdier Y, Vinh J, Hoffmann J A, Martin F, Baumert T F, Schuster C, Imler JL
RACK1 Controls IRES-Mediated Translation of Viruses. Article de journal
Dans: Cell, vol. 159, no. 5, p. 1086-1095, 2014, ISBN: 25416947.
Résumé | Liens | BibTeX | Étiquettes: ERIANI, hoffmann, imler, M3i, meignin, ROMBY, Unité ARN
@article{,
title = {RACK1 Controls IRES-Mediated Translation of Viruses.},
author = {K Majzoub and M L Hafirassou and C Meignin and A Goto and S Marzi and A Fedorova and Y Verdier and J Vinh and J A Hoffmann and F Martin and T F Baumert and C Schuster and JL Imler},
url = {http://www.ncbi.nlm.nih.gov/pubmed/25416947},
doi = {10.1016/j.cell.2014.10.041},
isbn = {25416947},
year = {2014},
date = {2014-01-01},
journal = {Cell},
volume = {159},
number = {5},
pages = {1086-1095},
abstract = {Fighting viral infections is hampered by the scarcity of viral targets and their variability, resulting in development of resistance. Viruses depend on cellular molecules-which are attractive alternative targets-for their life cycle, provided that they are dispensable for normal cell functions. Using the model organism Drosophila melanogaster, we identify the ribosomal protein RACK1 as a cellular factor required for infection by internal ribosome entry site (IRES)-containing viruses. We further show that RACK1 is an essential determinant for hepatitis C virus translation and infection, indicating that its function is conserved for distantly related human and fly viruses. Inhibition of RACK1 does not affect Drosophila or human cell viability and proliferation, and RACK1-silenced adult flies are viable, indicating that this protein is not essential for general translation. Our findings demonstrate a specific function for RACK1 in selective mRNA translation and uncover a target for the development of broad antiviral intervention.},
keywords = {ERIANI, hoffmann, imler, M3i, meignin, ROMBY, Unité ARN},
pubstate = {published},
tppubtype = {article}
}
2013
Petrillo Jessica E, Venter Arno P, Short James R, Gopal Radhika, Deddouche Safia, Lamiable Olivier, Imler Jean-Luc, Schneemann Anette
Cytoplasmic granule formation and translational inhibition of nodaviral RNAs in the absence of the double-stranded RNA binding protein B2 Article de journal
Dans: Journal of Virology, vol. 87, no. 24, p. 13409–13421, 2013, ISSN: 1098-5514.
Résumé | Liens | BibTeX | Étiquettes: Animals, Capsid Proteins, Cell Line, Cricetinae, Cytoplasmic Granules, Double-Stranded, imler, M3i, Nodaviridae, Protein Biosynthesis, RNA, RNA Virus Infections, RNA-Binding Proteins, Viral, Viral Proteins
@article{petrillo_cytoplasmic_2013,
title = {Cytoplasmic granule formation and translational inhibition of nodaviral RNAs in the absence of the double-stranded RNA binding protein B2},
author = {Jessica E Petrillo and Arno P Venter and James R Short and Radhika Gopal and Safia Deddouche and Olivier Lamiable and Jean-Luc Imler and Anette Schneemann},
doi = {10.1128/JVI.02362-13},
issn = {1098-5514},
year = {2013},
date = {2013-12-01},
journal = {Journal of Virology},
volume = {87},
number = {24},
pages = {13409--13421},
abstract = {Flock House virus (FHV) is a positive-sense RNA insect virus with a bipartite genome. RNA1 encodes the RNA-dependent RNA polymerase, and RNA2 encodes the capsid protein. A third protein, B2, is translated from a subgenomic RNA3 derived from the 3' end of RNA1. B2 is a double-stranded RNA (dsRNA) binding protein that inhibits RNA silencing, a major antiviral defense pathway in insects. FHV is conveniently propagated in Drosophila melanogaster cells but can also be grown in mammalian cells. It was previously reported that B2 is dispensable for FHV RNA replication in BHK21 cells; therefore, we chose this cell line to generate a viral mutant that lacked the ability to produce B2. Consistent with published results, we found that RNA replication was indeed vigorous but the yield of progeny virus was negligible. Closer inspection revealed that infected cells contained very small amounts of coat protein despite an abundance of RNA2. B2 mutants that had reduced affinity for dsRNA produced analogous results, suggesting that the dsRNA binding capacity of B2 somehow played a role in coat protein synthesis. Using fluorescence in situ hybridization of FHV RNAs, we discovered that RNA2 is recruited into large cytoplasmic granules in the absence of B2, whereas the distribution of RNA1 remains largely unaffected. We conclude that B2, by binding to double-stranded regions in progeny RNA2, prevents recruitment of RNA2 into cellular structures, where it is translationally silenced. This represents a novel function of B2 that further contributes to successful completion of the nodaviral life cycle.},
keywords = {Animals, Capsid Proteins, Cell Line, Cricetinae, Cytoplasmic Granules, Double-Stranded, imler, M3i, Nodaviridae, Protein Biosynthesis, RNA, RNA Virus Infections, RNA-Binding Proteins, Viral, Viral Proteins},
pubstate = {published},
tppubtype = {article}
}