@article{,
title = {Combining crystallogenesis methods to produce diffraction-quality crystals of a psychrophilic tRNA-maturation enzyme},
author = {R de Wijn and O Hennig and F G M Ernst and B Lorber and H Betat and M Mörl and C Sauter},
url = {https://www.ncbi.nlm.nih.gov/pubmed/30387781?dopt=Abstract},
doi = {10.1107/S2053230X18014590},
isbn = {30387781},
year = {2018},
date = {2018-01-01},
journal = {Acta Crystallogr F Struct Biol Commun},
volume = {74},
number = {Pt 11},
pages = {747-753},
abstract = {The determination of conditions for the reproducible growth of well diffracting crystals is a critical step in every biocrystallographic study. On the occasion of a new structural biology project, several advanced crystallogenesis approaches were tested in order to increase the success rate of crystallization. These methods included screening by microseed matrix screening, optimization by counter-diffusion and crystal detection by trace fluorescent labeling, and are easily accessible to any laboratory. Their combination proved to be particularly efficient in the case of the target, a 48 kDa CCA-adding enzyme from the psychrophilic bacterium Planococcus halocryophilus. A workflow summarizes the overall strategy, which led to the production of crystals that diffracted to better than 2 Å resolution and may be of general interest for a variety of applications.},
keywords = {CCA-adding enzyme Planococcus halocryophilus counter-diffusion crystallogenesis microseeding optimization tRNA maturation trace fluorescent labeling, SAUTER, Unité ARN},
pubstate = {published},
tppubtype = {article}
}
The determination of conditions for the reproducible growth of well diffracting crystals is a critical step in every biocrystallographic study. On the occasion of a new structural biology project, several advanced crystallogenesis approaches were tested in order to increase the success rate of crystallization. These methods included screening by microseed matrix screening, optimization by counter-diffusion and crystal detection by trace fluorescent labeling, and are easily accessible to any laboratory. Their combination proved to be particularly efficient in the case of the target, a 48 kDa CCA-adding enzyme from the psychrophilic bacterium Planococcus halocryophilus. A workflow summarizes the overall strategy, which led to the production of crystals that diffracted to better than 2 Å resolution and may be of general interest for a variety of applications.