Kurapati Rajendra, Muzi Laura, de Garibay Aritz Perez Ruiz, Russier Julie, Voiry Damien, Vacchi Isabella A, Chhowalla Manish, Bianco Alberto
Enzymatic Biodegradability of Pristine and Functionalized Transition Metal Dichalcogenide MoS2 Nanosheets Article de journal
Dans: Advanced Functional Materials, vol. 27, no. 7, p. 1605176, 2017, ISSN: 1616-3028.
Résumé | Liens | BibTeX | Étiquettes: Cytotoxicity, degradation, graphene-related materials, I2CT, molybdenum disulfide, peroxidases, Team-Bianco
@article{kurapati_enzymatic_2017,
title = {Enzymatic Biodegradability of Pristine and Functionalized Transition Metal Dichalcogenide MoS2 Nanosheets},
author = {Rajendra Kurapati and Laura Muzi and Aritz Perez Ruiz de Garibay and Julie Russier and Damien Voiry and Isabella A Vacchi and Manish Chhowalla and Alberto Bianco},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.201605176},
doi = {10.1002/adfm.201605176},
issn = {1616-3028},
year = {2017},
date = {2017-01-01},
urldate = {2020-04-01},
journal = {Advanced Functional Materials},
volume = {27},
number = {7},
pages = {1605176},
abstract = {2D transition metal dichalcogenide MoS2 nanosheets are increasingly attracting interests due to their promising applications in materials science and biomedicine. However, their biocompatibility and their biodegradability have not been thoroughly studied yet. Here, the biodegradability of exfoliated pristine and covalently functionalized MoS2 (f-MoS2) is investigated. First, biodegradability of these nanomaterials is evaluated using plant horseradish peroxidase and human myeloperoxidase. The results reveal that the enzymatic degradability rate of MoS2 and f-MoS2 is slower than in the case of the simple treatment with H2O2 alone. In parallel, high biocompatibility of both pristine and f-MoS2 nanosheets is found up to 100 µg mL−1 in both cell lines (HeLa and Raw264.7) and primary immune cells. In addition, no immune cell activation and minimal pro-inflammatory cytokine release are observed in RAW264.7 and human monocyte-derived macrophages, suggesting a negligible cellular impact of such materials. Furthermore, the effects of degraded MoS2 and partially degraded f-MoS2 products on cell viability and activation are studied in cancer and immune cells. A certain cytotoxicity is measured at the highest concentrations. Finally, to prove that the cellular impact is due to cell uptake, the internalization of both pristine and functionalized MoS2 in cancer and primary immune cells is assessed.},
keywords = {Cytotoxicity, degradation, graphene-related materials, I2CT, molybdenum disulfide, peroxidases, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Flacher Vincent, Tripp Christoph H, Mairhofer David G, Steinman Ralph M, Stoitzner Patrizia, Idoyaga Juliana, Romani Nikolaus
Murine Langerin+ dermal dendritic cells prime CD8+ Ŧ cells while Langerhans cells induce cross-tolerance Article de journal
Dans: EMBO molecular medicine, vol. 6, no. 9, p. 1191–1204, 2014, ISSN: 1757-4684.
Résumé | Liens | BibTeX | Étiquettes: agonists, Animals, Antibodies, antibody, Antigen, Antigen Presentation, Antigens, C-Type, C-type lectin, cancer, CD70, CD8-Positive T-Lymphocytes, CD8+ T cells, CD8+ T‐cell responses, Cellular, CROSS-PRESENTATION, Cross-Priming, Cytotoxicity, Dendritic Cells, DERMAL DENDRITIC CELLS, DERMATOLOGY, disease, imiquimod, Immunization, IMMUNOGENICITY, Immunologic Memory, Immunological, Immunology, In vivo, Inbred C57BL, INDUCTION, Intradermal, Langerhans Cells, LECTIN, Lectins, Mannose-Binding Lectins, Maturation, Mice, Models, murine, OVALBUMIN, physiology, priming, RESPONSES, Skin, Surface, T CELLS, T-CELLS, Team-Mueller, tolerance, Vaccination, vaccine, Vaccines
@article{flacher_murine_2014,
title = {Murine Langerin+ dermal dendritic cells prime CD8+ Ŧ cells while Langerhans cells induce cross-tolerance},
author = {Vincent Flacher and Christoph H Tripp and David G Mairhofer and Ralph M Steinman and Patrizia Stoitzner and Juliana Idoyaga and Nikolaus Romani},
doi = {10.15252/emmm.201303283},
issn = {1757-4684},
year = {2014},
date = {2014-09-01},
journal = {EMBO molecular medicine},
volume = {6},
number = {9},
pages = {1191--1204},
abstract = {Skin dendritic cells (DCs) control the immunogenicity of cutaneously administered vaccines. Antigens targeted to DCs via the C-type lectin Langerin/CD207 are cross-presented to CD8(+) T cells in vivo. We investigated the relative roles of Langerhans cells (LCs) and Langerin(+) dermal DCs (dDCs) in different vaccination settings. Poly(I:C) and anti-CD40 agonist antibody promoted cytotoxic responses upon intradermal immunization with ovalbumin (OVA)-coupled anti-Langerin antibodies (Langerin/OVA). This correlated with CD70 upregulation in Langerin(+) dDCs, but not LCs. In chimeric mice where Langerin targeting was restricted to dDCs, CD8(+) T-cell memory was enhanced. Conversely, providing Langerin/OVA exclusively to LCs failed to prime cytotoxicity, despite initial antigen cross-presentation to CD8(+) T cells. Langerin/OVA combined with imiquimod could not prime CD8(+) T cells and resulted in poor cytotoxicity in subsequent responses. This tolerance induction required targeting and maturation of LCs. Altogether, Langerin(+) dDCs prime long-lasting cytotoxic responses, while cross-presentation by LCs negatively influences CD8(+) T-cell priming. Moreover, this highlights that DCs exposed to TLR agonists can still induce tolerance and supports the existence of qualitatively different DC maturation programs.},
keywords = {agonists, Animals, Antibodies, antibody, Antigen, Antigen Presentation, Antigens, C-Type, C-type lectin, cancer, CD70, CD8-Positive T-Lymphocytes, CD8+ T cells, CD8+ T‐cell responses, Cellular, CROSS-PRESENTATION, Cross-Priming, Cytotoxicity, Dendritic Cells, DERMAL DENDRITIC CELLS, DERMATOLOGY, disease, imiquimod, Immunization, IMMUNOGENICITY, Immunologic Memory, Immunological, Immunology, In vivo, Inbred C57BL, INDUCTION, Intradermal, Langerhans Cells, LECTIN, Lectins, Mannose-Binding Lectins, Maturation, Mice, Models, murine, OVALBUMIN, physiology, priming, RESPONSES, Skin, Surface, T CELLS, T-CELLS, Team-Mueller, tolerance, Vaccination, vaccine, Vaccines},
pubstate = {published},
tppubtype = {article}
}
Gaillard Claire, Cellot Giada, Li Shouping, Toma Francesca Maria, Dumortier Hélène, Spalluto Giampiero, Cacciari Barbara, Prato Maurizio, Ballerini Laura, Bianco Alberto
Carbon Nanotubes Carrying Cell-Adhesion Peptides do not Interfere with Neuronal Functionality Article de journal
Dans: Advanced Materials, vol. 21, no. 28, p. 2903–2908, 2009, ISSN: 1521-4095.
Résumé | Liens | BibTeX | Étiquettes: Carbon nanotubes, Cytotoxicity, I2CT, mammalian cells, Neurons, Peptides, Team-Bianco
@article{gaillard_carbon_2009,
title = {Carbon Nanotubes Carrying Cell-Adhesion Peptides do not Interfere with Neuronal Functionality},
author = {Claire Gaillard and Giada Cellot and Shouping Li and Francesca Maria Toma and Hélène Dumortier and Giampiero Spalluto and Barbara Cacciari and Maurizio Prato and Laura Ballerini and Alberto Bianco},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.200900050},
doi = {10.1002/adma.200900050},
issn = {1521-4095},
year = {2009},
date = {2009-01-01},
urldate = {2020-03-31},
journal = {Advanced Materials},
volume = {21},
number = {28},
pages = {2903--2908},
abstract = {Water-soluble carbon nanotubes functionalized with cell-adhesion peptides do not affect the viability of different cell types, including Jurkat cells, splenocytes, and neurons. They also do not modify the neuronal morphology and basic functions, thus representing a promising candidate for the exploitation of novel drug-delivery systems or for designing a new generation of self-assembling nerve bridges.},
keywords = {Carbon nanotubes, Cytotoxicity, I2CT, mammalian cells, Neurons, Peptides, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Lacotte Stéphanie, García Ainara, Décossas Marion, Al‐Jamal Wafa' T, Li Shouping, Kostarelos Kostas, Muller Sylviane, Prato Maurizio, Dumortier Hélène, Bianco Alberto
Interfacing Functionalized Carbon Nanohorns with Primary Phagocytic Cells Article de journal
Dans: Advanced Materials, vol. 20, no. 12, p. 2421–2426, 2008, ISSN: 1521-4095.
Résumé | Liens | BibTeX | Étiquettes: carbon nanostructures, Cells, Cytotoxicity, Drug delivery, I2CT, Team-Bianco
@article{lacotte_interfacing_2008,
title = {Interfacing Functionalized Carbon Nanohorns with Primary Phagocytic Cells},
author = {Stéphanie Lacotte and Ainara García and Marion Décossas and Wafa' T Al‐Jamal and Shouping Li and Kostas Kostarelos and Sylviane Muller and Maurizio Prato and Hélène Dumortier and Alberto Bianco},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.200702753},
doi = {10.1002/adma.200702753},
issn = {1521-4095},
year = {2008},
date = {2008-01-01},
urldate = {2020-03-31},
journal = {Advanced Materials},
volume = {20},
number = {12},
pages = {2421--2426},
abstract = {Functionalized carbon nanohorns (f-CNH) are uptaken by macrophages, without affecting cell viability. f-CNH induce the production of reactive oxygen species and of pro-inflammatory cytokines. The level of inflammation, although moderate, should be taken into consideration when using f-CNH for drug delivery. However, it could be exploited as an intrinsic f-CNH adjuvant function for biomedical applications requiring some activation of the immune system.},
keywords = {carbon nanostructures, Cells, Cytotoxicity, Drug delivery, I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Dumortier Hélène, van Mierlo Geertje J D, Egan Deirdre, van Ewijk Willem, Toes René E M, Offringa Rienk, Melief Cornelis J M
Dans: Journal of Immunology (Baltimore, Md.: 1950), vol. 175, no. 2, p. 855–863, 2005, ISSN: 0022-1767.
Résumé | Liens | BibTeX | Étiquettes: Adenovirus E1A Proteins, Animals, Antigen, Antigen Presentation, CD8-Positive T-Lymphocytes, Cell Differentiation, Cell Line, Cell Movement, Clonal Deletion, Cytotoxic, Cytotoxicity, Dendritic Cells, Down-Regulation, Dumortier, Epitopes, Female, I2CT, Immunologic, Immunologic Memory, Inbred C57BL, Lipopolysaccharides, Lymphocyte Activation, Mice, Myeloid Cells, Receptors, Regulatory, T-Cell, T-Lymphocyte, T-Lymphocytes, Team-Dumortier, transgenic
@article{dumortier_antigen_2005,
title = {Antigen presentation by an immature myeloid dendritic cell line does not cause CTL deletion in vivo, but generates CD8+ central memory-like Ŧ cells that can be rescued for full effector function},
author = {Hélène Dumortier and Geertje J D van Mierlo and Deirdre Egan and Willem van Ewijk and René E M Toes and Rienk Offringa and Cornelis J M Melief},
doi = {10.4049/jimmunol.175.2.855},
issn = {0022-1767},
year = {2005},
date = {2005-01-01},
journal = {Journal of Immunology (Baltimore, Md.: 1950)},
volume = {175},
number = {2},
pages = {855--863},
abstract = {Immature dendritic cells (DC), in contrast to their mature counterparts, are incapable of mobilizing a CD8+ CTL response, and, instead, have been reported to induce CTL tolerance. We directly addressed the impact of immature vs mature DC on CTL responses by infusing adenovirus peptide-loaded DC (of the D1 cell line) into mice that had received adenovirus-specific naive TCR-transgenic CD8+ T cells. Whereas i.v. injection of mature DC triggered vigorous CTL expansion, immature DC elicited little proliferation involving only a minority of the TCR-transgenic CTL. Even though the latter CTL developed effector functions, including cytolytic activity and proinflammatory cytokine secretion, these cells differed significantly from CTL primed by mature DC in that they did not exhibit down-regulation of CD62L and CCR7, receptors involved in trapping of T cells in the lymphoid organs. Interestingly, adoptive transfer of CTL effector cells harvested after priming by either mature or immature DC into naive recipient mice, followed by exposure to adenovirus, yielded quantitatively and qualitatively indistinguishable CTL memory responses. Therefore, in vivo priming of naive CD8+ T cells by immature DC, although failing to induce a full-blown, systemic CTL response, resulted in the formation of central memory-like T cells that were able to expand and produce IFN-gamma upon secondary antigenic stimulation.},
keywords = {Adenovirus E1A Proteins, Animals, Antigen, Antigen Presentation, CD8-Positive T-Lymphocytes, Cell Differentiation, Cell Line, Cell Movement, Clonal Deletion, Cytotoxic, Cytotoxicity, Dendritic Cells, Down-Regulation, Dumortier, Epitopes, Female, I2CT, Immunologic, Immunologic Memory, Inbred C57BL, Lipopolysaccharides, Lymphocyte Activation, Mice, Myeloid Cells, Receptors, Regulatory, T-Cell, T-Lymphocyte, T-Lymphocytes, Team-Dumortier, transgenic},
pubstate = {published},
tppubtype = {article}
}
van Mierlo Geertje J D, Boonman Zita F H M, Dumortier Hélène M H, den Boer Annemieke Th, Fransen Marieke F, Nouta Jan, van der Voort Ellen I H, Offringa Rienk, Toes René E M, Melief Cornelis J M
Activation of dendritic cells that cross-present tumor-derived antigen licenses CD8+ CTL to cause tumor eradication Article de journal
Dans: Journal of Immunology (Baltimore, Md.: 1950), vol. 173, no. 11, p. 6753–6759, 2004, ISSN: 0022-1767.
Résumé | Liens | BibTeX | Étiquettes: Adenovirus E1A Proteins, Animals, Antibodies, Antigen-Presenting Cells, Antigens, CD11c Antigen, CD40 Antigens, Cross-Priming, Cultured, Cytotoxic, Cytotoxicity, Dendritic Cells, Dumortier, Epitopes, Experimental, I2CT, Immunologic, Inbred C57BL, Injections, Intralesional, Intravenous, Knockout, Male, Mice, Monoclonal, Neoplasms, T-Lymphocyte, T-Lymphocytes, Team-Dumortier, transgenic, tumor, Tumor Cells, Viral
@article{van_mierlo_activation_2004,
title = {Activation of dendritic cells that cross-present tumor-derived antigen licenses CD8+ CTL to cause tumor eradication},
author = {Geertje J D van Mierlo and Zita F H M Boonman and Hélène M H Dumortier and Annemieke Th den Boer and Marieke F Fransen and Jan Nouta and Ellen I H van der Voort and Rienk Offringa and René E M Toes and Cornelis J M Melief},
doi = {10.4049/jimmunol.173.11.6753},
issn = {0022-1767},
year = {2004},
date = {2004-12-01},
journal = {Journal of Immunology (Baltimore, Md.: 1950)},
volume = {173},
number = {11},
pages = {6753--6759},
abstract = {The fate of naive CD8(+) T cells is determined by the environment in which they encounter MHC class I presented peptide Ags. The manner in which tumor Ags are presented is a longstanding matter of debate. Ag presentation might be mediated by tumor cells in tumor draining lymph nodes or via cross-presentation by professional APC. Either pathway is insufficient to elicit protective antitumor immunity. We now demonstrate using a syngeneic mouse tumor model, expressing an Ag derived from the early region 1A of human adenovirus type 5, that the inadequate nature of the antitumor CTL response is not due to direct Ag presentation by the tumor cells, but results from presentation of tumor-derived Ag by nonactivated CD11c(+) APC. Although this event results in division of naive CTL in tumor draining lymph nodes, it does not establish a productive immune response. Treatment of tumor-bearing mice with dendritic cell-stimulating agonistic anti-CD40 mAb resulted in systemic efflux of CTL with robust effector function capable to eradicate established tumors. For efficacy of anti-CD40 treatment, CD40 ligation of host APC is required because adoptive transfer of CD40-proficient tumor-specific TCR transgenic CTL into CD40-deficient tumor-bearing mice did not lead to productive antitumor immunity after CD40 triggering in vivo. CpG and detoxified LPS (MPL) acted similarly as agonistic anti-CD40 mAb with respect to CD8(+) CTL efflux and tumor eradication. Together these results indicate that dendritic cells, depending on their activation state, orchestrate the outcome of CTL-mediated immunity against tumors, leading either to an ineffective immune response or potent antitumor immunity.},
keywords = {Adenovirus E1A Proteins, Animals, Antibodies, Antigen-Presenting Cells, Antigens, CD11c Antigen, CD40 Antigens, Cross-Priming, Cultured, Cytotoxic, Cytotoxicity, Dendritic Cells, Dumortier, Epitopes, Experimental, I2CT, Immunologic, Inbred C57BL, Injections, Intralesional, Intravenous, Knockout, Male, Mice, Monoclonal, Neoplasms, T-Lymphocyte, T-Lymphocytes, Team-Dumortier, transgenic, tumor, Tumor Cells, Viral},
pubstate = {published},
tppubtype = {article}
}