Myslinski E, Schuster C, Krol A, Carbon P
Promoter strength and structure dictate module composition in RNA polymerase III transcriptional activator elements Article de journal
Dans: J Mol Biol, vol. 234, no. 2, p. 311-318, 1993, ISBN: 7693950, (0022-2836 Journal Article).
Résumé | Liens | BibTeX | Étiquettes: Animals Base Sequence Molecular Sequence Data Mutagenesis, Genetic/*physiology Xenopus laevis, Non-U.S. Gov't Transcription, Nucleic Acid/*physiology Selenocysteine/genetics Support, Site-Directed Oocytes/metabolism Promoter Regions (Genetics)/*genetics RNA/*genetics RNA Polymerase III/*metabolism RNA, Small Nuclear/genetics RNA, Transfer/genetics Regulatory Sequences, Unité ARN
@article{,
title = {Promoter strength and structure dictate module composition in RNA polymerase III transcriptional activator elements},
author = {E Myslinski and C Schuster and A Krol and P Carbon},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7693950},
isbn = {7693950},
year = {1993},
date = {1993-01-01},
journal = {J Mol Biol},
volume = {234},
number = {2},
pages = {311-318},
abstract = {RNA polymerase III transcription of genes with external promoters only (e.g. U6 snRNA) or containing in addition an internal B box (selenocysteine tRNA(Sec)) is stimulated by upstream elements; a distal sequence element (DSE) for U6 or an activator element in the tRNA(Sec) gene. In contrast to the composite structure of the DSE which requires an octamer motif, the Xenopus tRNA(Sec) activator element contains an SPH motif only. In vivo transcription is optimally stimulated by SPH in an absolute octamer-independent manner since adding octamer does not induce superstimulation. Experiments performed in the work presented here led to the following observations. Co-operation between SPH and octamer motifs can be detected in two distinct cases: first when these motifs are placed in front of B box-less tRNA(Sec) or U6 external promoters and second, if either element of the external promoter (proximal sequence element or TATA element), or the SPH motif itself, are altered. Altogether, our data provide evidence that an SPH motif can function alone in an optimized promoter only. In contrast, an octamer becomes indispensable when the basal promoter is weak or disabled. It follows that module composition of Pol III transcriptional activator elements is dependent on the structure and strength of the promoter. This reveals the existence of cross-talk between activator and promoter elements, mediated by the bound transcription factors, which are thus able to compensate for each other in order to allow successful assembly of the transcription complex.},
note = {0022-2836
Journal Article},
keywords = {Animals Base Sequence Molecular Sequence Data Mutagenesis, Genetic/*physiology Xenopus laevis, Non-U.S. Gov't Transcription, Nucleic Acid/*physiology Selenocysteine/genetics Support, Site-Directed Oocytes/metabolism Promoter Regions (Genetics)/*genetics RNA/*genetics RNA Polymerase III/*metabolism RNA, Small Nuclear/genetics RNA, Transfer/genetics Regulatory Sequences, Unité ARN},
pubstate = {published},
tppubtype = {article}
}
Myslinski E, Krol A, Carbon P
Optimal tRNA((Ser)Sec) gene activity requires an upstream SPH motif Article de journal
Dans: Nucleic Acids Res, vol. 20, no. 2, p. 203-209, 1992, ISBN: 1311068, (0305-1048 Journal Article).
Résumé | Liens | BibTeX | Étiquettes: Amino Acid-Specific/*genetics Recombinant Fusion Proteins/genetics/metabolism Simian virus 40/*genetics Support, Animals Base Sequence DNA Mutational Analysis DNA-Binding Proteins/genetics Enhancer Elements (Genetics)/*genetics/physiology Gene Expression Regulation/*genetics Molecular Sequence Data Promoter Regions (Genetics)/genetics RNA, Non-U.S. Gov't TATA Box/genetics Xenopus laevis/*genetics, Small Nuclear/genetics RNA, Transfer, Unité ARN
@article{,
title = {Optimal tRNA((Ser)Sec) gene activity requires an upstream SPH motif},
author = {E Myslinski and A Krol and P Carbon},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1311068},
isbn = {1311068},
year = {1992},
date = {1992-01-01},
journal = {Nucleic Acids Res},
volume = {20},
number = {2},
pages = {203-209},
abstract = {The X. laevis tRNA((Ser)Sec) gene is different from the other tRNA genes in that its promoter contains two external elements, a PSE and a TATA box functionally equivalent to those of the U6 snRNA gene. Of the two internal promoters governing classical tRNA gene transcription, only subsists the internal B box. In this report, we show that the tRNA((Ser)Sec) contains in addition an activator element (AE) which we have mapped by extensive mutagenesis. Activation is only dependent on a 15 bp fragment residing between -209 and -195 and containing an SPH motif. In vitro, this element forms a complex with a nuclear protein which is different from the TEF-1 transcriptional activator that binds the SV40 Sph motifs. This AE is versatile since it shows capacity of activating a variety of genes in vivo, including U1 and U6 snRNAs and HSV thymidine kinase. Unexpectedly for an snRNA-related gene, the tRNA((Ser)Sec) is deprived of octamer or octamer-like motifs. The X.laevis tRNA((Ser)Sec) gene represents the first example of a Pol III snRNA-type gene whose activation of transcription is completely octamer-independent.},
note = {0305-1048
Journal Article},
keywords = {Amino Acid-Specific/*genetics Recombinant Fusion Proteins/genetics/metabolism Simian virus 40/*genetics Support, Animals Base Sequence DNA Mutational Analysis DNA-Binding Proteins/genetics Enhancer Elements (Genetics)/*genetics/physiology Gene Expression Regulation/*genetics Molecular Sequence Data Promoter Regions (Genetics)/genetics RNA, Non-U.S. Gov't TATA Box/genetics Xenopus laevis/*genetics, Small Nuclear/genetics RNA, Transfer, Unité ARN},
pubstate = {published},
tppubtype = {article}
}