Wang-Sattler Rui, Blandin Stephanie A, Ning Ye, Blass Claudia, Dolo Guimogo, Touré Yeya T, delle Torre Alessandra, Lanzaro Gregory C, Steinmetz Lars M, Kafatos Fotis C, Zheng Liangbiao
Mosaic genome architecture of the Anopheles gambiae species complex Article de journal
Dans: PLoS ONE, vol. 2, no. 11, p. e1249, 2007, ISSN: 1932-6203.
Résumé | Liens | BibTeX | Étiquettes: Animals, Anopheles gambiae, Artificial, Bacterial, Biological Evolution, blandin, Chromosomes, Female, Genetic Markers, Genetic Variation, Genome, M3i, Microsatellite Repeats, Mosaicism
@article{wang-sattler_mosaic_2007,
title = {Mosaic genome architecture of the Anopheles gambiae species complex},
author = {Rui Wang-Sattler and Stephanie A Blandin and Ye Ning and Claudia Blass and Guimogo Dolo and Yeya T Touré and Alessandra delle Torre and Gregory C Lanzaro and Lars M Steinmetz and Fotis C Kafatos and Liangbiao Zheng},
doi = {10.1371/journal.pone.0001249},
issn = {1932-6203},
year = {2007},
date = {2007-01-01},
journal = {PLoS ONE},
volume = {2},
number = {11},
pages = {e1249},
abstract = {BACKGROUND: Attempts over the last three decades to reconstruct the phylogenetic history of the Anopheles gambiae species complex have been important for developing better strategies to control malaria transmission. METHODOLOGY: We used fingerprint genotyping data from 414 field-collected female mosquitoes at 42 microsatellite loci to infer the evolutionary relationships of four species in the A. gambiae complex, the two major malaria vectors A. gambiae sensu stricto (A. gambiae s.s.) and A. arabiensis, as well as two minor vectors, A. merus and A. melas. PRINCIPAL FINDINGS: We identify six taxonomic units, including a clear separation of West and East Africa A. gambiae s.s. S molecular forms. We show that the phylogenetic relationships vary widely between different genomic regions, thus demonstrating the mosaic nature of the genome of these species. The two major malaria vectors are closely related and closer to A. merus than to A. melas at the genome-wide level, which is also true if only autosomes are considered. However, within the Xag inversion region of the X chromosome, the M and two S molecular forms are most similar to A. merus. Near the X centromere, outside the Xag region, the two S forms are highly dissimilar to the other taxa. Furthermore, our data suggest that the centromeric region of chromosome 3 is a strong discriminator between the major and minor malaria vectors. CONCLUSIONS: Although further studies are needed to elucidate the basis of the phylogenetic variation among the different regions of the genome, the preponderance of sympatric admixtures among taxa strongly favor introgression of different genomic regions between species, rather than lineage sorting of ancestral polymorphism, as a possible mechanism.},
keywords = {Animals, Anopheles gambiae, Artificial, Bacterial, Biological Evolution, blandin, Chromosomes, Female, Genetic Markers, Genetic Variation, Genome, M3i, Microsatellite Repeats, Mosaicism},
pubstate = {published},
tppubtype = {article}
}
Manfruelli P, Reichhart Jean-Marc, Steward R, Hoffmann Jules A, Lemaitre Bruno
A mosaic analysis in Drosophila fat body cells of the control of antimicrobial peptide genes by the Rel proteins Dorsal and DIF Article de journal
Dans: EMBO J., vol. 18, no. 12, p. 3380–3391, 1999, ISSN: 0261-4189.
Résumé | Liens | BibTeX | Étiquettes: Animals, Anti-Infective Agents, Cell Surface, Clone Cells, DNA-Binding Proteins, Fat Body, Female, Gene Expression Regulation, Genes, hoffmann, Insect, Insect Proteins, Larva, M3i, Male, Membrane Glycoproteins, Mosaicism, Mutation, Nuclear Proteins, Phosphoproteins, Receptors, reichhart, Reporter, Signal Transduction, Toll-Like Receptors, Transcription Factors
@article{manfruelli_mosaic_1999,
title = {A mosaic analysis in Drosophila fat body cells of the control of antimicrobial peptide genes by the Rel proteins Dorsal and DIF},
author = {P Manfruelli and Jean-Marc Reichhart and R Steward and Jules A Hoffmann and Bruno Lemaitre},
doi = {10.1093/emboj/18.12.3380},
issn = {0261-4189},
year = {1999},
date = {1999-06-01},
journal = {EMBO J.},
volume = {18},
number = {12},
pages = {3380--3391},
abstract = {Expression of the gene encoding the antifungal peptide Drosomycin in Drosophila adults is controlled by the Toll signaling pathway. The Rel proteins Dorsal and DIF (Dorsal-related immunity factor) are possible candidates for the transactivating protein in the Toll pathway that directly regulates the drosomycin gene. We have examined the requirement of Dorsal and DIF for drosomycin expression in larval fat body cells, the predominant immune-responsive tissue, using the yeast site-specific flp/FRT recombination system to generate cell clones homozygous for a deficiency uncovering both the dorsal and the dif genes. Here we show that in the absence of both genes, the immune-inducibility of drosomycin is lost but can be rescued by overexpression of either dorsal or dif under the control of a heat-shock promoter. This result suggests a functional redundancy between both Rel proteins in the control of drosomycin gene expression in the larvae of Drosophila. Interestingly, the gene encoding the antibacterial peptide Diptericin remains fully inducible in the absence of the dorsal and dif genes. Finally, we have used fat body cell clones homozygous for various mutations to show that a linear activation cascade Spaetzle--textgreater Toll--textgreaterCactus--textgreaterDorsal/DIF leads to the induction of the drosomycin gene in larval fat body cells.},
keywords = {Animals, Anti-Infective Agents, Cell Surface, Clone Cells, DNA-Binding Proteins, Fat Body, Female, Gene Expression Regulation, Genes, hoffmann, Insect, Insect Proteins, Larva, M3i, Male, Membrane Glycoproteins, Mosaicism, Mutation, Nuclear Proteins, Phosphoproteins, Receptors, reichhart, Reporter, Signal Transduction, Toll-Like Receptors, Transcription Factors},
pubstate = {published},
tppubtype = {article}
}