Serag Maged F, Kaji Noritada, Gaillard Claire, Okamoto Yukihiro, Terasaka Kazuyoshi, Jabasini Mohammad, Tokeshi Manabu, Mizukami Hajime, Bianco Alberto, Baba Yoshinobu
Trafficking and subcellular localization of multiwalled carbon nanotubes in plant cells Article de journal
Dans: ACS nano, vol. 5, no. 1, p. 493–499, 2011, ISSN: 1936-086X.
Résumé | Liens | BibTeX | Étiquettes: Biological Transport, carbon, Catharanthus, Cell Membrane, Endosomes, I2CT, Intracellular Space, Nanotubes, Protoplasts, Team-Bianco
@article{serag_trafficking_2011,
title = {Trafficking and subcellular localization of multiwalled carbon nanotubes in plant cells},
author = {Maged F Serag and Noritada Kaji and Claire Gaillard and Yukihiro Okamoto and Kazuyoshi Terasaka and Mohammad Jabasini and Manabu Tokeshi and Hajime Mizukami and Alberto Bianco and Yoshinobu Baba},
doi = {10.1021/nn102344t},
issn = {1936-086X},
year = {2011},
date = {2011-01-01},
journal = {ACS nano},
volume = {5},
number = {1},
pages = {493--499},
abstract = {Major barriers to delivery of biomolecules are crossing the cellular membranes and achieving a high cytoplasmic concentration by circumventing entrapment into endosomes and other lytic organelles. Motivated by such aim, we have investigated the capability of multiwalled carbon nanotubes (MWCNTs) to penetrate the cell membrane of plant protoplasts (plant cells made devoid of their cell walls via enzymatic treatment) and studied their internalization mechanism via confocal imaging and TEM techniques. Our results indentified an endosome-escaping uptake mode of MWCNTs by plant protoplasts. Moreover, short MWCNTs (textbackslashtextless100 nm) were observed to target specific cellular substructures including the nucleus, plastids, and vacuoles. These findings are expected to have a significant impact on plant cell biology and transformation technologies.},
keywords = {Biological Transport, carbon, Catharanthus, Cell Membrane, Endosomes, I2CT, Intracellular Space, Nanotubes, Protoplasts, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Benincasa Monica, Pacor Sabrina, Wu Wei, Prato Maurizio, Bianco Alberto, Gennaro Renato
Antifungal activity of amphotericin B conjugated to carbon nanotubes Article de journal
Dans: ACS nano, vol. 5, no. 1, p. 199–208, 2011, ISSN: 1936-086X.
Résumé | Liens | BibTeX | Étiquettes: Amphotericin B, Antifungal Agents, Candida, carbon, Cell Membrane, Deoxycholic Acid, Drug Design, Drug Resistance, Fungal, Humans, I2CT, Jurkat Cells, Kinetics, Membrane Potentials, Nanotubes, Team-Bianco
@article{benincasa_antifungal_2011,
title = {Antifungal activity of amphotericin B conjugated to carbon nanotubes},
author = {Monica Benincasa and Sabrina Pacor and Wei Wu and Maurizio Prato and Alberto Bianco and Renato Gennaro},
doi = {10.1021/nn1023522},
issn = {1936-086X},
year = {2011},
date = {2011-01-01},
journal = {ACS nano},
volume = {5},
number = {1},
pages = {199--208},
abstract = {Amphotericin B (AMB) has long been considered the most effective drug in the treatment of serious invasive fungal infections. There are, however, major limitations to its use, due to several adverse effects, including acute infusional reactions and, most relevant, a dose-dependent nephrotoxicity. At least some of these effects are attributed to the aggregation of AMB as a result of its poor water solubility. To overcome this problem, reformulated versions of the drug have been developed, including a micellar dispersion of AMB with sodium deoxycholate (AMBD), its encapsulation into liposomes, or its incorporation into lipidic complexes. The development of nanobiotechnologies provides novel potential drug delivery systems that make use of nanomaterials such as functionalized carbon nanotubes (f-CNTs), which are emerging as an innovative and efficient tool for the transport and cellular translocation of therapeutic molecules. In this study, we prepared two conjugates between f-CNTs and AMB. The antifungal activity of these conjugates was tested against a collection of reference and clinical fungal strains, in comparison to that of AMB alone or AMBD. Measured minimum inhibition concentration (MIC) values for f-CNT-AMB conjugates were either comparable to or better than those displayed by AMB and AMBD. Furthermore, AMBD-resistant Candida strains were found to be susceptible to f-CNT-AMB 1. Additional studies, aimed at understanding the mechanism of action of the conjugates, suggest a nonlytic mechanism, since the compounds show a major permeabilizing effect on the tested fungal strains only after extended incubation. Interestingly, the f-CNT-AMB 1 does not show any significant toxic effect on Jurkat cells at antifungal concentrations.},
keywords = {Amphotericin B, Antifungal Agents, Candida, carbon, Cell Membrane, Deoxycholic Acid, Drug Design, Drug Resistance, Fungal, Humans, I2CT, Jurkat Cells, Kinetics, Membrane Potentials, Nanotubes, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Herrero Antonia M, Lacerda Lara, Bianco Alberto, Kostarelos Kostas, Prato Maurizio
Functionalised carbon nanotubes: high biocompatibility with lack of toxicity Article de journal
Dans: International Journal of Nanotechnology, vol. 8, no. 10/11/12, p. 885, 2011, ISSN: 1475-7435, 1741-8151.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{herrero_functionalised_2011,
title = {Functionalised carbon nanotubes: high biocompatibility with lack of toxicity},
author = {Antonia M Herrero and Lara Lacerda and Alberto Bianco and Kostas Kostarelos and Maurizio Prato},
url = {http://www.inderscience.com/link.php?id=44433},
doi = {10.1504/IJNT.2011.044433},
issn = {1475-7435, 1741-8151},
year = {2011},
date = {2011-01-01},
urldate = {2020-04-01},
journal = {International Journal of Nanotechnology},
volume = {8},
number = {10/11/12},
pages = {885},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Cellot Giada, Ballerini Laura, Prato Maurizio, Bianco Alberto
Neurons are able to internalize soluble carbon nanotubes: new opportunities or old risks? Article de journal
Dans: Small (Weinheim an Der Bergstrasse, Germany), vol. 6, no. 23, p. 2630–2633, 2010, ISSN: 1613-6829.
Liens | BibTeX | Étiquettes: carbon, Cell Line, Cells, Cultured, Humans, I2CT, Nanotubes, Neurons, Team-Bianco, tumor
@article{cellot_neurons_2010,
title = {Neurons are able to internalize soluble carbon nanotubes: new opportunities or old risks?},
author = {Giada Cellot and Laura Ballerini and Maurizio Prato and Alberto Bianco},
doi = {10.1002/smll.201000906},
issn = {1613-6829},
year = {2010},
date = {2010-12-01},
journal = {Small (Weinheim an Der Bergstrasse, Germany)},
volume = {6},
number = {23},
pages = {2630--2633},
keywords = {carbon, Cell Line, Cells, Cultured, Humans, I2CT, Nanotubes, Neurons, Team-Bianco, tumor},
pubstate = {published},
tppubtype = {article}
}
Al-Jamal Khuloud T, Toma Francesca M, Yilmazer Açelya, Ali-Boucetta Hanene, Nunes Antonio, Herrero Maria-Antonia, Tian Bowen, Eddaoudi Ayad, Eddaoui Ayad, Al-Jamal Wafa' T, Bianco Alberto, Prato Maurizio, Kostarelo Kostas
Enhanced cellular internalization and gene silencing with a series of cationic dendron-multiwalled carbon nanotube:siRNA complexes Article de journal
Dans: FASEB journal: official publication of the Federation of American Societies for Experimental Biology, vol. 24, no. 11, p. 4354–4365, 2010, ISSN: 1530-6860.
Résumé | Liens | BibTeX | Étiquettes: Biological Transport, carbon, Cations, Cell Line, Cell Survival, Gene Silencing, HeLa Cells, Humans, I2CT, Models, Molecular, Nanotubes, RNA, Small Interfering, Team-Bianco, Transfection, tumor
@article{al-jamal_enhanced_2010,
title = {Enhanced cellular internalization and gene silencing with a series of cationic dendron-multiwalled carbon nanotube:siRNA complexes},
author = {Khuloud T Al-Jamal and Francesca M Toma and Açelya Yilmazer and Hanene Ali-Boucetta and Antonio Nunes and Maria-Antonia Herrero and Bowen Tian and Ayad Eddaoudi and Ayad Eddaoui and Wafa' T Al-Jamal and Alberto Bianco and Maurizio Prato and Kostas Kostarelo},
doi = {10.1096/fj.09-141036},
issn = {1530-6860},
year = {2010},
date = {2010-11-01},
journal = {FASEB journal: official publication of the Federation of American Societies for Experimental Biology},
volume = {24},
number = {11},
pages = {4354--4365},
abstract = {One of the major obstacles to the clinical development of gene silencing by small interfering RNA (siRNA) is its effective cytoplasmic delivery. Carbon nanotubes have been proposed as novel nanomaterials that can offer significant advantages for the intracellular delivery of nucleic acids, such as siRNA. We recently demonstrated in a proof-of-principle study that amino-functionalized multiwalled carbon nanotubes (f-MWNT) can effectively deliver in vivo an siRNA sequence, triggering cell apoptosis that results in human lung xenograft eradication and prolonged survival. In the present study, we demonstrate how a newly synthesized series of polycationic dendron-MWNT constructs with a precisely tailored number of amino functions (dendron generations) can complex and effectively deliver double-stranded siRNA to achieve gene silencing in vitro. A systematic comparison between the f-MWNT series in terms of cellular uptake, cytotoxicity, and siRNA complexation is offered. Significant improvement in siRNA delivery with the dendron-MWNT conjugates is shown, and gene silencing was obtained in 2 human cell lines using 2 different siRNA sequences. The study reveals that through f-MWNT structure-biological function analysis novel nanotube-based siRNA transfer vectors can be designed with minimal cytotoxicity and effective delivery and gene-silencing capabilities.},
keywords = {Biological Transport, carbon, Cations, Cell Line, Cell Survival, Gene Silencing, HeLa Cells, Humans, I2CT, Models, Molecular, Nanotubes, RNA, Small Interfering, Team-Bianco, Transfection, tumor},
pubstate = {published},
tppubtype = {article}
}
den Bossche Jeroen Van, Al-Jamal Wafa' T, Tian Bowen, Nunes Antonio, Fabbro Chiara, Bianco Alberto, Prato Maurizio, Kostarelos Kostas
Efficient receptor-independent intracellular translocation of aptamers mediated by conjugation to carbon nanotubes Article de journal
Dans: Chemical Communications (Cambridge, England), vol. 46, no. 39, p. 7379–7381, 2010, ISSN: 1364-548X.
Résumé | Liens | BibTeX | Étiquettes: Aptamers, Base Sequence, Biological Transport, carbon, Cell Line, Cell Surface, DNA Primers, Electron, Electrophoresis, Humans, I2CT, Microscopy, Nanotubes, Nucleotide, Polyacrylamide Gel, Receptors, Team-Bianco, Transmission, tumor
@article{van_den_bossche_efficient_2010,
title = {Efficient receptor-independent intracellular translocation of aptamers mediated by conjugation to carbon nanotubes},
author = {Jeroen Van den Bossche and Wafa' T Al-Jamal and Bowen Tian and Antonio Nunes and Chiara Fabbro and Alberto Bianco and Maurizio Prato and Kostas Kostarelos},
doi = {10.1039/c0cc02092c},
issn = {1364-548X},
year = {2010},
date = {2010-10-01},
journal = {Chemical Communications (Cambridge, England)},
volume = {46},
number = {39},
pages = {7379--7381},
abstract = {We have covalently grafted aptamers onto carboxylated carbon nanotubes to design a novel vector system that can easily translocate into the cytosol of different cell types independent of receptor-mediated uptake. We propose the use of carbon nanotubes for the efficient intracellular delivery of biologically active aptamers for potential therapeutic applications.},
keywords = {Aptamers, Base Sequence, Biological Transport, carbon, Cell Line, Cell Surface, DNA Primers, Electron, Electrophoresis, Humans, I2CT, Microscopy, Nanotubes, Nucleotide, Polyacrylamide Gel, Receptors, Team-Bianco, Transmission, tumor},
pubstate = {published},
tppubtype = {article}
}
Samorì Cristian, Sainz Raquel, Ménard-Moyon Cécilia, Toma Francesca M, Venturelli Enrica, Singh Prabhpreet, Ballestri Marco, Prato Maurizio, Bianco Alberto
Potentiometric titration as a straightforward method to assess the number of functional groups on shortened carbon nanotubes Article de journal
Dans: Carbon, vol. 48, no. 9, p. 2447–2454, 2010, ISSN: 0008-6223.
Résumé | Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{samori_potentiometric_2010,
title = {Potentiometric titration as a straightforward method to assess the number of functional groups on shortened carbon nanotubes},
author = {Cristian Samorì and Raquel Sainz and Cécilia Ménard-Moyon and Francesca M Toma and Enrica Venturelli and Prabhpreet Singh and Marco Ballestri and Maurizio Prato and Alberto Bianco},
url = {http://www.sciencedirect.com/science/article/pii/S0008622310001806},
doi = {10.1016/j.carbon.2010.03.015},
issn = {0008-6223},
year = {2010},
date = {2010-08-01},
urldate = {2020-03-31},
journal = {Carbon},
volume = {48},
number = {9},
pages = {2447--2454},
abstract = {The conditions for oxidizing multi-walled carbon nanotubes to shorten them to a narrow length distribution have been optimized. One of the most difficult achievements is to fully characterize this material from a chemical point of view, and to find a good quantitative correlation among different techniques. Herein, we report on the combination of different methods to determine the number of functional groups generated during strong acid treatment and a further amidation reaction. A good correlation was found using the colorimetric Kaiser test, thermogravimetric analysis and potentiometric argentometric titration. The final technique, used for the first time in this field, is highly versatile and, being non-destructive, allows a complete recovery of the starting material. Short carbon nanotubes are particularly useful for applications in biomedicine, and the control and precise assessment of their functionalization is critical when used as carriers for therapeutic molecules.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Fabre Bruno, Ababou-Girard Soraya, Singh Prabhpreet, Kumar Jitendra, Verma Sandeep, Bianco Alberto
Noncovalent assembly of ferrocene on modified gold surfaces mediated by uracil–adenine base pairs Article de journal
Dans: Electrochemistry Communications, vol. 12, no. 6, p. 831–834, 2010, ISSN: 1388-2481.
Résumé | Liens | BibTeX | Étiquettes: Electrochemistry, Ferrocene, Gold surface, I2CT, Monolayer, Nucleobases, Team-Bianco
@article{fabre_noncovalent_2010,
title = {Noncovalent assembly of ferrocene on modified gold surfaces mediated by uracil–adenine base pairs},
author = {Bruno Fabre and Soraya Ababou-Girard and Prabhpreet Singh and Jitendra Kumar and Sandeep Verma and Alberto Bianco},
url = {http://www.sciencedirect.com/science/article/pii/S1388248110001451},
doi = {10.1016/j.elecom.2010.03.045},
issn = {1388-2481},
year = {2010},
date = {2010-06-01},
urldate = {2020-04-01},
journal = {Electrochemistry Communications},
volume = {12},
number = {6},
pages = {831--834},
abstract = {Redox-active ferrocene was assembled on gold surfaces through the hydrogen bonding interactions between adenine-substituted ferrocene and a uracil-terminated organothiol monolayer. The surface coverage of ferrocene Γ could be varied from ca. 4×10−11 to 2.0×10−10molcm−2 by diluting the thiol-modified uracil derivative with inert 1-octanethiol. A decrease in the apparent electron transfer rate constant for ferrocene, kapp, from ca. 50 to 10s−1 was observed upon increasing Γ.},
keywords = {Electrochemistry, Ferrocene, Gold surface, I2CT, Monolayer, Nucleobases, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Kostarelos K, Bianco A, Prato M
Complement monitoring of carbon nanotubes Article de journal
Dans: Nature Nanotechnology, vol. 5, no. 6, p. 382–383, 2010, ISSN: 1748-3395.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{kostarelos_complement_2010,
title = {Complement monitoring of carbon nanotubes},
author = {K Kostarelos and A Bianco and M Prato},
url = {https://www.nature.com/articles/nnano.2010.110},
doi = {10.1038/nnano.2010.110},
issn = {1748-3395},
year = {2010},
date = {2010-06-01},
urldate = {2020-04-01},
journal = {Nature Nanotechnology},
volume = {5},
number = {6},
pages = {382--383},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Lacotte Stéphanie, Dumortier Hélène, Décossas Marion, Briand Jean-Paul, Muller Sylviane
Identification of new pathogenic players in lupus: autoantibody-secreting cells are present in nephritic kidneys of (NZBxNZW)F1 mice Article de journal
Dans: Journal of Immunology (Baltimore, Md.: 1950), vol. 184, no. 7, p. 3937–3945, 2010, ISSN: 1550-6606.
Résumé | Liens | BibTeX | Étiquettes: Animals, Autoantibodies, Autoantigens, B-Lymphocytes, Dumortier, Enzyme-Linked Immunosorbent Assay, Female, Histones, I2CT, Immunoblotting, Immunohistochemistry, Inbred BALB C, Inbred NZB, Lupus Nephritis, Mice, Team-Dumortier
@article{lacotte_identification_2010,
title = {Identification of new pathogenic players in lupus: autoantibody-secreting cells are present in nephritic kidneys of (NZBxNZW)F1 mice},
author = {Stéphanie Lacotte and Hélène Dumortier and Marion Décossas and Jean-Paul Briand and Sylviane Muller},
doi = {10.4049/jimmunol.0902595},
issn = {1550-6606},
year = {2010},
date = {2010-04-01},
journal = {Journal of Immunology (Baltimore, Md.: 1950)},
volume = {184},
number = {7},
pages = {3937--3945},
abstract = {An important hallmark of systemic lupus erythematosus is the production of autoantibodies specific for nuclear Ags, among which nucleosomes and their constituents, DNA and histones. It is widely admitted that some of these autoantibodies contribute largely in lupus pathogenesis because of their nephritogenic potential. However, the underlying mechanisms are still debated. In this study, we analyzed the autoimmune response against histone H2B during the course of the disease in lupus-prone (NZBxNZW)F1 mice, both in lymphoid organs and kidneys, and we assessed its potential involvement in lupus pathogenicity. We found that the N-terminal region of histone H2B represents a preferential target for circulating autoantibodies, which kinetics of appearance positively correlates with disease development. Furthermore, immunization of preautoimmune (NZBxNZW)F1 mice with H2B peptide 1-25 accelerates the disease. Kidney eluates from diseased (NZBxNZW)F1 mice do contain IgG Abs reacting with this peptide, and this H2B sequence was found to be accessible to specific Ab probes in Ag-containing deposits detected in nephritic kidneys. Finally, compared with control normal mice and to young preautoimmune (NZBxNZW)F1 animals, the frequency of cells secreting autoantibodies reacting with peptide 1-25 was significantly raised in the spleen and bone marrow and most importantly on a pathophysiological point of view, locally, in nephritic kidneys of diseased (NZBxNZW)F1 mice. Altogether our results demonstrate the existence in (NZBxNZW)F1 mice of both a systemic and local B cell response targeting the N-terminal region of histone H2B, and highlight the potential implication of this nuclear domain in lupus pathology.},
keywords = {Animals, Autoantibodies, Autoantigens, B-Lymphocytes, Dumortier, Enzyme-Linked Immunosorbent Assay, Female, Histones, I2CT, Immunoblotting, Immunohistochemistry, Inbred BALB C, Inbred NZB, Lupus Nephritis, Mice, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}
Marega Riccardo, Aroulmoji Vincent, Bergamin Massimo, Feruglio Luigi, Dinon Francesca, Bianco Alberto, Murano Erminio, Prato Maurizio
Two-dimensional diffusion-ordered NMR spectroscopy as a tool for monitoring functionalized carbon nanotube purification and composition Article de journal
Dans: ACS nano, vol. 4, no. 4, p. 2051–2058, 2010, ISSN: 1936-086X.
Résumé | Liens | BibTeX | Étiquettes: carbon, Diffusion, I2CT, Magnetic Resonance Spectroscopy, Nanotubes, Polyethylene Glycols, Solubility, Team-Bianco, Temperature, water
@article{marega_two-dimensional_2010,
title = {Two-dimensional diffusion-ordered NMR spectroscopy as a tool for monitoring functionalized carbon nanotube purification and composition},
author = {Riccardo Marega and Vincent Aroulmoji and Massimo Bergamin and Luigi Feruglio and Francesca Dinon and Alberto Bianco and Erminio Murano and Maurizio Prato},
doi = {10.1021/nn100257h},
issn = {1936-086X},
year = {2010},
date = {2010-04-01},
journal = {ACS nano},
volume = {4},
number = {4},
pages = {2051--2058},
abstract = {Functionalized carbon nanotube (CNT) derivatives are currently under thorough investigation in different biomedical investigations. In this field of research, the composition of sample either in terms of covalently attached or physisorbed moieties can greatly affect the observed results and hamper the comparison between different studies. Therefore, the availability of a fast and reliable analytical technique to assess both the type of interaction (covalent vs noncovalent) and the composition of CNT conjugates is of great importance. Here we describe that the two-dimensional diffusion-ordered (DOSY) NMR spectroscopy is extremely useful to discriminate between conjugated and unconjugated polyethylene glycol groups in samples obtained by condensation with oxidized single-walled carbon nanotubes (SWNTs). This fast and nondestructive technique allows us to follow the removal of unconjugated polyethylene glycol chains during the purification. In particular, DOSY analysis reveal that about 1/3 (wt %) of the polyethylene glycol used for the condensation remained physisorbed to functionalized SWNTs after dialysis. Complete elimination of physisorbed polyethylene glycol was achieved using diafiltration.},
keywords = {carbon, Diffusion, I2CT, Magnetic Resonance Spectroscopy, Nanotubes, Polyethylene Glycols, Solubility, Team-Bianco, Temperature, water},
pubstate = {published},
tppubtype = {article}
}
Samorì Cristian, Ali-Boucetta Hanene, Sainz Raquel, Guo Chang, Toma Francesca Maria, Fabbro Chiara, da Ros Tatiana, Prato Maurizio, Kostarelos Kostas, Bianco Alberto
Enhanced anticancer activity of multi-walled carbon nanotube-methotrexate conjugates using cleavable linkers Article de journal
Dans: Chemical Communications (Cambridge, England), vol. 46, no. 9, p. 1494–1496, 2010, ISSN: 1364-548X.
Résumé | Liens | BibTeX | Étiquettes: Antineoplastic Agents, Azo Compounds, carbon, Cell Line, Cross-Linking Reagents, Humans, I2CT, Methotrexate, Nanotubes, Team-Bianco, Thiosemicarbazones, tumor
@article{samori_enhanced_2010,
title = {Enhanced anticancer activity of multi-walled carbon nanotube-methotrexate conjugates using cleavable linkers},
author = {Cristian Samorì and Hanene Ali-Boucetta and Raquel Sainz and Chang Guo and Francesca Maria Toma and Chiara Fabbro and Tatiana da Ros and Maurizio Prato and Kostas Kostarelos and Alberto Bianco},
doi = {10.1039/b923560d},
issn = {1364-548X},
year = {2010},
date = {2010-03-01},
journal = {Chemical Communications (Cambridge, England)},
volume = {46},
number = {9},
pages = {1494--1496},
abstract = {Methotrexate was tethered to multi-walled carbon nanotubes through different cleavable linkers exploiting the ammonium functionalities introduced by 1,3-dipolar cycloaddition reaction of azomethine ylides to the nanotubes. The new nanobio-hybrid conjugates were internalized into human breast cancer cells and it was shown that the cytotoxic activity was strongly dependent on the presence and type of linker.},
keywords = {Antineoplastic Agents, Azo Compounds, carbon, Cell Line, Cross-Linking Reagents, Humans, I2CT, Methotrexate, Nanotubes, Team-Bianco, Thiosemicarbazones, tumor},
pubstate = {published},
tppubtype = {article}
}
Geotti-Bianchini Piero, Moretto Alessandro, Peggion Cristina, Beyrath Julien, Bianco Alberto, Formaggio Fernando
Replacement of Ala by Aib improves structuration and biological stability in thymine-based α-nucleopeptides Article de journal
Dans: Organic & Biomolecular Chemistry, vol. 8, no. 6, p. 1315–1321, 2010, ISSN: 1477-0539.
Résumé | Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{geotti-bianchini_replacement_2010,
title = {Replacement of Ala by Aib improves structuration and biological stability in thymine-based α-nucleopeptides},
author = {Piero Geotti-Bianchini and Alessandro Moretto and Cristina Peggion and Julien Beyrath and Alberto Bianco and Fernando Formaggio},
url = {https://pubs.rsc.org/en/content/articlelanding/2010/ob/b920211k},
doi = {10.1039/B920211K},
issn = {1477-0539},
year = {2010},
date = {2010-03-01},
urldate = {2020-03-31},
journal = {Organic & Biomolecular Chemistry},
volume = {8},
number = {6},
pages = {1315--1321},
abstract = {Three thymine-based nucleo-heptapeptides, each containing two nucleo-amino acids and zero, one or four Aib residues, respectively, have been synthesized. A single Aib residue is enough to promote the adoption of a helical structure in our nucleopeptides and to increase significantly their resistance towards enzymatic degradation. The insertion of four Aib residues, out of seven residues in the sequence, affords a rigid, 310-helical nucleopeptide that is substantially unaffected by serum enzymes and is not cytotoxic.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Al-Jamal Khuloud T, Al-Jamal Wafa’ T, Akerman Simon, Podesta Jennifer E, Yilmazer Açelya, Turton John A, Bianco Alberto, Vargesson Neil, Kanthou Chryso, Florence Alexander T, Tozer Gillian M, Kostarelos Kostas
Systemic antiangiogenic activity of cationic poly-L-lysine dendrimer delays tumor growth Article de journal
Dans: Proceedings of the National Academy of Sciences, vol. 107, no. 9, p. 3966–3971, 2010, ISSN: 0027-8424, 1091-6490.
Résumé | Liens | BibTeX | Étiquettes: angiogenesis, cancer, I2CT, nanoparticle, Team-Bianco
@article{al-jamal_systemic_2010,
title = {Systemic antiangiogenic activity of cationic poly-L-lysine dendrimer delays tumor growth},
author = {Khuloud T Al-Jamal and Wafa’ T Al-Jamal and Simon Akerman and Jennifer E Podesta and Açelya Yilmazer and John A Turton and Alberto Bianco and Neil Vargesson and Chryso Kanthou and Alexander T Florence and Gillian M Tozer and Kostas Kostarelos},
url = {https://www.pnas.org/content/107/9/3966},
doi = {10.1073/pnas.0908401107},
issn = {0027-8424, 1091-6490},
year = {2010},
date = {2010-03-01},
urldate = {2020-04-01},
journal = {Proceedings of the National Academy of Sciences},
volume = {107},
number = {9},
pages = {3966--3971},
abstract = {This study describes the previously unreported intrinsic capacity of poly-L-lysine (PLL) sixth generation (G6) dendrimer molecules to exhibit systemic antiangiogenic activity that could lead to solid tumor growth arrest. The PLL-dendrimer-inhibited tubule formation of SVEC4-10 murine endothelial cells and neovascularization in the chick embryo chick chorioallantoic membrane (CAM) assay. Intravenous administration of the PLL-dendrimer molecules into C57BL/6 mice inhibited vascularisation in Matrigel plugs implanted subcutaneously. Antiangiogenic activity was further evidenced using intravital microscopy of tumors grown within dorsal skinfold window chambers. Reduced vascularization of P22 rat sarcoma implanted in the dorsal window chamber of SCID mice was observed following tail vein administration (i.v.) of the PLL dendrimers. Also, the in vivo toxicological profile of the PLL-dendrimer molecules was shown to be safe at the dose regime studied. The antiangiogenic activity of the PLL dendrimer was further shown to be associated with significant suppression of B16F10 solid tumor volume and delayed tumor growth. Enhanced apoptosis/necrosis within tumors of PLL-dendrimer-treated animals only and reduction in the number of CD31 positive cells were observed in comparison to protamine treatment. This study suggests that PLL-dendrimer molecules can exhibit a systemic antiangiogenic activity that may be used for therapy of solid tumors, and in combination with their capacity to carry other therapeutic or diagnostic agents may potentially offer capabilities for the design of theranostic systems.},
keywords = {angiogenesis, cancer, I2CT, nanoparticle, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Ben-Valid Shoshana, Dumortier Hélène, Décossas Marion, Sfez Ruthy, Meneghetti Moreno, Bianco Alberto, Yitzchaik Shlomo
Polyaniline-coated single-walled carbon nanotubes: synthesis, characterization and impact on primary immune cells Article de journal
Dans: Journal of Materials Chemistry, vol. 20, no. 12, p. 2408–2417, 2010, ISSN: 1364-5501.
Résumé | Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{ben-valid_polyaniline-coated_2010,
title = {Polyaniline-coated single-walled carbon nanotubes: synthesis, characterization and impact on primary immune cells},
author = {Shoshana Ben-Valid and Hélène Dumortier and Marion Décossas and Ruthy Sfez and Moreno Meneghetti and Alberto Bianco and Shlomo Yitzchaik},
url = {https://pubs.rsc.org/en/content/articlelanding/2010/jm/b921828a},
doi = {10.1039/B921828A},
issn = {1364-5501},
year = {2010},
date = {2010-03-01},
urldate = {2020-04-01},
journal = {Journal of Materials Chemistry},
volume = {20},
number = {12},
pages = {2408--2417},
abstract = {Functionalized carbon nanotubes are increasingly exploited as innovative components for the development of advanced biomedical devices. In this study we report a novel synthetic route for the formation of single-walled carbon nanotube (SWCNT)–polyaniline (PANI) hybrids by in situ chemical polymerization. The surfactant sodium dodecylsulfate (SDS) is used as a template for monomer assembly and polymerization. The resulting composite preserves the surfactant and is characterized by a tight binding between SWCNTs and PANI. Having the idea of integrating these new types of SWCNT conjugates into advanced biomedical tools (i.e. implantable multi-electrode arrays), we explored their potential impact on the viability and function of cells from the immune system. We have compared the cytotoxic effects of SWCNT-COOH, SWCNT/SDS and SWCNT/SDS/PANI on mouse spleen cells and macrophages. The results indicate that biocompatibility of the different SWCNT conjugates is dependent both on the doses used and the type of cells.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Ménard-Moyon Cécilia, Kostarelos Kostas, Prato Maurizio, Bianco Alberto
Functionalized carbon nanotubes for probing and modulating molecular functions Article de journal
Dans: Chemistry & Biology, vol. 17, no. 2, p. 107–115, 2010, ISSN: 1879-1301.
Résumé | Liens | BibTeX | Étiquettes: Antibodies, Antigens, Atomic Force, Biosensing Techniques, carbon, Drug Delivery Systems, enzymes, Glycoproteins, I2CT, Ion Channels, Microscopy, Nanotubes, RNA, Small Interfering, Team-Bianco
@article{menard-moyon_functionalized_2010,
title = {Functionalized carbon nanotubes for probing and modulating molecular functions},
author = {Cécilia Ménard-Moyon and Kostas Kostarelos and Maurizio Prato and Alberto Bianco},
doi = {10.1016/j.chembiol.2010.01.009},
issn = {1879-1301},
year = {2010},
date = {2010-02-01},
journal = {Chemistry & Biology},
volume = {17},
number = {2},
pages = {107--115},
abstract = {Carbon nanotubes (CNTs) entered the domain of biological research a few years ago, creating a significant amount of interest due to their extraordinary physicochemical properties. The integration of CNT-based strategies with biology necessitates a multidisciplinary approach that requires competences in the diverse fields of chemistry, physics, and life sciences. In the biomedical domain CNTs are extensively explored as novel drug delivery systems for therapy and diagnosis. Additionally, CNTs can also be designed as new tools for modulation of molecular functions, by directly affecting various biological processes or by interaction with bioactive molecules. The aim of this review is to discuss how CNTs can be exploited as new probes for molecular functions. The different sections illustrate various applications of CNTs, including gene silencing, surface cell interactions via glycoproteins, biosensing, intracellular drug delivery using an atomic force microscopy tip-based nanoinjector, modulation of antibody/antigen interaction and enzyme activity, and blocking of ion channels.},
keywords = {Antibodies, Antigens, Atomic Force, Biosensing Techniques, carbon, Drug Delivery Systems, enzymes, Glycoproteins, I2CT, Ion Channels, Microscopy, Nanotubes, RNA, Small Interfering, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Parietti Véronique, Chifflot Hélène, Sibilia Jean, Muller Sylviane, Monneaux Fanny
Rituximab treatment overcomes reduction of regulatory iNKT cells in patients with rheumatoid arthritis Article de journal
Dans: Clinical Immunology (Orlando, Fla.), vol. 134, no. 3, p. 331–339, 2010, ISSN: 1521-7035.
Résumé | Liens | BibTeX | Étiquettes: Adult, Age Factors, Aged, Antibodies, Antirheumatic Agents, arthritis, Female, Flow Cytometry, Humans, I2CT, Longitudinal Studies, Male, Middle Aged, Monneaux, Monoclonal, Murine-Derived, Natural Killer T-Cells, Nonparametric, rheumatoid, Rituximab, Sex Factors, Statistics, Team-Dumortier, Young Adult
@article{parietti_rituximab_2010,
title = {Rituximab treatment overcomes reduction of regulatory iNKT cells in patients with rheumatoid arthritis},
author = {Véronique Parietti and Hélène Chifflot and Jean Sibilia and Sylviane Muller and Fanny Monneaux},
doi = {10.1016/j.clim.2009.11.007},
issn = {1521-7035},
year = {2010},
date = {2010-01-01},
journal = {Clinical Immunology (Orlando, Fla.)},
volume = {134},
number = {3},
pages = {331--339},
abstract = {Invariant natural killer T (iNKT) cells are a subset of T cells that recognize glycolipid antigens presented by the CD1d molecule. Accumulating evidences showed that iNKT cells are implicated in the regulatory mechanisms that control autoimmunity. We evaluated the number of circulating iNKT cells in patients with rheumatoid arthritis (RA) by flow cytometry and performed a longitudinal analysis of iNKT cell frequency in RA patients who were given an anti-CD20 therapy. Significantly lower iNKT cell numbers were measured in the blood from RA patients compared to healthy individuals (ptextless0.0001) and low iNKT cell frequencies were rather associated with an active disease. In RA patients who received rituximab treatment, iNKT cell number was increased in relation to the clinical outcome. We demonstrated that the number of iNKT cells is altered in RA patients and that following rituximab therapy, clinical remission of RA is associated with an increase of iNKT cell frequency.},
keywords = {Adult, Age Factors, Aged, Antibodies, Antirheumatic Agents, arthritis, Female, Flow Cytometry, Humans, I2CT, Longitudinal Studies, Male, Middle Aged, Monneaux, Monoclonal, Murine-Derived, Natural Killer T-Cells, Nonparametric, rheumatoid, Rituximab, Sex Factors, Statistics, Team-Dumortier, Young Adult},
pubstate = {published},
tppubtype = {article}
}
Ménard-Moyon Cécilia, Venturelli Enrica, Fabbro Chiara, Samorì Cristian, Ros Tatiana Da, Kostarelos Kostas, Prato Maurizio, Bianco Alberto
The alluring potential of functionalized carbon nanotubes in drug discovery Article de journal
Dans: Expert Opinion on Drug Discovery, vol. 5, no. 7, p. 691–707, 2010, ISSN: 1746-0441.
Résumé | Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{menard-moyon_alluring_2010,
title = {The alluring potential of functionalized carbon nanotubes in drug discovery},
author = {Cécilia Ménard-Moyon and Enrica Venturelli and Chiara Fabbro and Cristian Samorì and Tatiana Da Ros and Kostas Kostarelos and Maurizio Prato and Alberto Bianco},
doi = {10.1517/17460441.2010.490552},
issn = {1746-0441},
year = {2010},
date = {2010-01-01},
journal = {Expert Opinion on Drug Discovery},
volume = {5},
number = {7},
pages = {691--707},
abstract = {IMPORTANCE OF THE FIELD: The possibility of carbon nanotube integration into living systems for therapeutic and diagnostic purposes has opened the way to explore their applications in drug delivery and discovery. A wide variety of chemical approaches has been developed to functionalize carbon nanotubes with therapeutic molecules towards different biomedical uses. AREAS COVERED IN THIS REVIEW: This review covers the recent advances in the development of functionalized carbon nanotubes to offer improvements for different diseases, in particular for cancer therapy. WHAT THE READER WILL GAIN: Functionalized carbon nanotubes are able to transport therapeutic agents. Targeted methodologies using carbon nanotube-based conjugates have been investigated to improve the efficacy of some drugs. The capacity of such nanomaterials to seamlessly translocate into cells with alternative various mechanisms and their pharmacokinetic properties is also discussed. TAKE HOME MESSAGE: Although at its infancy, functionalized carbon nanotubes are very promising as a new nanomedicine platform in the field of drug discovery and delivery. They have the capacity to cross biological barriers and can be eliminated via renal and/or fecal excretion. They can transport small drug molecules while maintaining - and in some cases improving - their therapeutic efficacy.},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Partidos Charalambos D, Hoebeke Johan, Wieckowski Sébastien, Chaloin Olivier, Bianco Alberto, Moreau Emmanuel, Briand Jean-Paul, Desgranges Claude, Muller Sylviane
Immunomodulatory consequences of ODN CpG-polycation complexes Article de journal
Dans: Methods (San Diego, Calif.), vol. 49, no. 4, p. 328–333, 2009, ISSN: 1095-9130.
Résumé | Liens | BibTeX | Étiquettes: Animals, carbon, CpG Islands, Humans, I2CT, Immunologic Factors, Nanotubes, Oligodeoxyribonucleotides, Polyamines, Team-Bianco, Transcriptional Activation
@article{partidos_immunomodulatory_2009,
title = {Immunomodulatory consequences of ODN CpG-polycation complexes},
author = {Charalambos D Partidos and Johan Hoebeke and Sébastien Wieckowski and Olivier Chaloin and Alberto Bianco and Emmanuel Moreau and Jean-Paul Briand and Claude Desgranges and Sylviane Muller},
doi = {10.1016/j.ymeth.2009.03.005},
issn = {1095-9130},
year = {2009},
date = {2009-12-01},
journal = {Methods (San Diego, Calif.)},
volume = {49},
number = {4},
pages = {328--333},
abstract = {Immunostimulatory ODN CpGs have extensively been tested as adjuvants and immunotherapeutics and hold a lot of promise for human use. In our studies we took advantage of their negative charge to study their biological activities after being complexed with carbon nanotubes, a novel vector for vaccine delivery and Tat protein of HIV, a target protein for therapeutic or prophylactic intervention. In the case of carbon nanotubes, ODN CpGs were able to form stable complexes based on charge interaction and exert increased immunostimulatory activity in vitro. With regard to the Tat protein, ODN CpGs were shown to bind effectively through the basic domain of the protein representing residues 44-61. Moreover, using surface Plasmon Resonance Technology and an in vitro cellular system, ODN CpGs were shown to inhibit the interaction of Tat protein with the transactivation responsive element, a bulged RNA hairpin structure. However, when ODN CpGs were complexed with Tat they readily increased the apoptotic properties of this protein as studied in CD3-stimulated Jurkat cells. Overall, our findings together with published data support the view that for harnessing the beneficial effects of ODN CpGs a careful consideration has to be given depending on the target intervention.},
keywords = {Animals, carbon, CpG Islands, Humans, I2CT, Immunologic Factors, Nanotubes, Oligodeoxyribonucleotides, Polyamines, Team-Bianco, Transcriptional Activation},
pubstate = {published},
tppubtype = {article}
}
Chamouard Patrick, Monneaux Fanny, Richert Zoe, Voegeli Anne-Claire, Lavaux Thomas, Gaub Marie Pierre, Baumann René, Oudet Pierre, Muller Sylviane
Diminution of Circulating CD4+CD25 high Ŧ cells in naïve Crohn's disease Article de journal
Dans: Digestive Diseases and Sciences, vol. 54, no. 10, p. 2084–2093, 2009, ISSN: 1573-2568.
Résumé | Liens | BibTeX | Étiquettes: Adult, Aged, Blood Cell Count, CD4 Antigens, Colitis, Crohn Disease, Female, Flow Cytometry, Humans, I2CT, Interleukin-2 Receptor alpha Subunit, Lymphocyte Subsets, Male, Middle Aged, Monneaux, Regulatory, T-Lymphocytes, Team-Dumortier, Ulcerative
@article{chamouard_diminution_2009,
title = {Diminution of Circulating CD4+CD25 high Ŧ cells in naïve Crohn's disease},
author = {Patrick Chamouard and Fanny Monneaux and Zoe Richert and Anne-Claire Voegeli and Thomas Lavaux and Marie Pierre Gaub and René Baumann and Pierre Oudet and Sylviane Muller},
doi = {10.1007/s10620-008-0590-6},
issn = {1573-2568},
year = {2009},
date = {2009-10-01},
journal = {Digestive Diseases and Sciences},
volume = {54},
number = {10},
pages = {2084--2093},
abstract = {Crohn's disease is considered to be caused either by an excess of T-cell effector functions and/or by a defective regulatory T-cell compartment. The aim of this study was to assess in Crohn's disease the frequency of circulating CD4(+)CD25(high) T cells that possess regulatory T-cell functions and CD4(+)CD25(low) T cells that contain activated T cells. Flow cytometry of peripheral blood was used to assess CD4(+)CD25(high) and CD4(+)CD25(low) T-cell frequencies in a cohort of 66 patients with Crohn's disease in comparison to 19 patients with ulcerative colitis and 31 healthy individuals enrolled as controls. The CD4(+)CD25(high) T-cell frequency was significantly lowered in naïve Crohn's disease (P = 0.013) and in ulcerative colitis (P = 0.001). CD4(+)CD25(low) T-cell frequency was increased in Crohn's disease (P = 0.0001) and in ulcerative colitis (P = 0.0002). Both CD4(+)CD25(high) and CD4(+)CD25(low) T-cell frequencies are altered in naïve Crohn's disease resulting in an imbalance between both populations and a relative contraction of the CD4(+)CD25(high) T-cell population.},
keywords = {Adult, Aged, Blood Cell Count, CD4 Antigens, Colitis, Crohn Disease, Female, Flow Cytometry, Humans, I2CT, Interleukin-2 Receptor alpha Subunit, Lymphocyte Subsets, Male, Middle Aged, Monneaux, Regulatory, T-Lymphocytes, Team-Dumortier, Ulcerative},
pubstate = {published},
tppubtype = {article}
}
Zacchigna Marina, Klumpp Cedric, Prato Maurizio, Bianco Alberto
In vitro behavior of multifunctionalized fullerene-warfarin conjugates Article de journal
Dans: Journal of Nanoscience and Nanotechnology, vol. 9, no. 10, p. 6210–6221, 2009, ISSN: 1533-4880.
Résumé | Liens | BibTeX | Étiquettes: Animals, Anticoagulants, Fullerenes, I2CT, In Vitro Techniques, Mice, Spectrophotometry, Team-Bianco, Ultraviolet, Warfarin
@article{zacchigna_vitro_2009,
title = {In vitro behavior of multifunctionalized fullerene-warfarin conjugates},
author = {Marina Zacchigna and Cedric Klumpp and Maurizio Prato and Alberto Bianco},
doi = {10.1166/jnn.2009.1551},
issn = {1533-4880},
year = {2009},
date = {2009-10-01},
journal = {Journal of Nanoscience and Nanotechnology},
volume = {9},
number = {10},
pages = {6210--6221},
abstract = {In this study we have covalently linked the anticoagulant warfarin to polyfunctionalized fullerenes. The objective was to explore the possibility of modifying the biological profile of a drug by covalent binding to functionalized fullerene. We have chosen warfarin as a model compound because it a widely used drug. We have analyzed the stability in vitro of the conjugates and found that the drug is released from the carbon support only after incubation in mouse plasma.},
keywords = {Animals, Anticoagulants, Fullerenes, I2CT, In Vitro Techniques, Mice, Spectrophotometry, Team-Bianco, Ultraviolet, Warfarin},
pubstate = {published},
tppubtype = {article}
}
Kostarelos K, Bianco A, Prato M
Promises, facts and challenges for carbon nanotubes in imaging and therapeutics Article de journal
Dans: Nature Nanotechnology, vol. 4, no. 10, p. 627–633, 2009, ISSN: 1748-3395.
Résumé | Liens | BibTeX | Étiquettes: Animals, carbon, Diagnostic Imaging, Drug Evaluation, Humans, I2CT, Nanomedicine, Nanotubes, Preclinical, Team-Bianco, therapeutics
@article{kostarelos_promises_2009,
title = {Promises, facts and challenges for carbon nanotubes in imaging and therapeutics},
author = {K Kostarelos and A Bianco and M Prato},
doi = {10.1038/nnano.2009.241},
issn = {1748-3395},
year = {2009},
date = {2009-10-01},
journal = {Nature Nanotechnology},
volume = {4},
number = {10},
pages = {627--633},
abstract = {The use of carbon nanotubes in medicine is now at the crossroads between a proof-of-principle concept and an established preclinical candidate for a variety of therapeutic and diagnostic applications. Progress towards clinical trials will depend on the outcomes of efficacy and toxicology studies, which will provide the necessary risk-to-benefit assessments for carbon-nanotube-based materials. Here we focus on carbon nanotubes that have been studied in preclinical animal models, and draw attention to the promises, facts and challenges of these materials as they transition from research to the clinical phase. We address common questions regarding the use of carbon nanotubes in disease imaging and therapy, and highlight the opportunities and challenges ahead.},
keywords = {Animals, carbon, Diagnostic Imaging, Drug Evaluation, Humans, I2CT, Nanomedicine, Nanotubes, Preclinical, Team-Bianco, therapeutics},
pubstate = {published},
tppubtype = {article}
}
Singh Prabhpreet, Kumar Jitendra, Toma Francesca Maria, Raya Jesus, Prato Maurizio, Fabre Bruno, Verma Sandeep, Bianco Alberto
Synthesis and characterization of nucleobase-carbon nanotube hybrids Article de journal
Dans: Journal of the American Chemical Society, vol. 131, no. 37, p. 13555–13562, 2009, ISSN: 1520-5126.
Résumé | Liens | BibTeX | Étiquettes: Adenine, Amides, Amines, Biosensing Techniques, carbon, Catalysis, Electrochemistry, Graphite, I2CT, Magnetic Resonance Spectroscopy, Nanotubes, Nanowires, Surface Properties, Team-Bianco
@article{singh_synthesis_2009,
title = {Synthesis and characterization of nucleobase-carbon nanotube hybrids},
author = {Prabhpreet Singh and Jitendra Kumar and Francesca Maria Toma and Jesus Raya and Maurizio Prato and Bruno Fabre and Sandeep Verma and Alberto Bianco},
doi = {10.1021/ja905041b},
issn = {1520-5126},
year = {2009},
date = {2009-09-01},
journal = {Journal of the American Chemical Society},
volume = {131},
number = {37},
pages = {13555--13562},
abstract = {We report the synthesis and characterization of adenine-single-walled carbon nanotube (SWCNT) hybrid materials, where for the first time nucleobases are covalently attached to the exosurface of SWCNTs. The structural properties of all hybrids have been characterized using usual spectroscopic and microscopic techniques. The degree of functional groups for functionalized SWCNTs (f-SWCNTs) 2a and 2b is one adenine group for each 26 and 37 carbon atoms, respectively. Solid-state magic angle spinning (13)C NMR spectroscopy (MAS NMR) and electrochemistry have been also applied for the characterization of these f-SWCNTs. AFM images of f-SWCNT 2b showed an interesting feature of horizontally aligned nanotubes along the surface when deposited on highly oriented pyrolytic graphite surface. Furthermore, we evaluated the coordinating ability of these hybrid materials toward silver ions, and interestingly, we found a pattern of silver nanoparticles localized over the surface of the carbon nanotube network. The presence of aligned and randomly oriented CNTs and their ability to coordinate with metal ions make this class of materials very interesting for applications in the development of novel electronic devices and as new supports for different catalytic transformations.},
keywords = {Adenine, Amides, Amines, Biosensing Techniques, carbon, Catalysis, Electrochemistry, Graphite, I2CT, Magnetic Resonance Spectroscopy, Nanotubes, Nanowires, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Singh Prabhpreet, Campidelli Stéphane, Giordani Silvia, Bonifazi Davide, Bianco Alberto, Prato Maurizio
Organic functionalisation and characterisation of single-walled carbon nanotubes Article de journal
Dans: Chemical Society Reviews, vol. 38, no. 8, p. 2214–2230, 2009, ISSN: 1460-4744.
Résumé | Liens | BibTeX | Étiquettes: Alkylation, carbon, Esterification, Free Radicals, Halogenation, I2CT, Microscopy, Nanotubes, Oxidation-Reduction, Spectrum Analysis, Team-Bianco
@article{singh_organic_2009,
title = {Organic functionalisation and characterisation of single-walled carbon nanotubes},
author = {Prabhpreet Singh and Stéphane Campidelli and Silvia Giordani and Davide Bonifazi and Alberto Bianco and Maurizio Prato},
doi = {10.1039/b518111a},
issn = {1460-4744},
year = {2009},
date = {2009-08-01},
journal = {Chemical Society Reviews},
volume = {38},
number = {8},
pages = {2214--2230},
abstract = {Since carbon nanotubes (CNTs) display unique structures and remarkable physical properties, a variety of applications have emerged in both materials and life sciences. In terms of applications, the functionalisation of nanotubes is extremely important, as it increases their solubility and processability, and combines the unique properties of single-walled carbon nanotubes (SWCNTs) with those of other classes of materials. A number of methods have been developed, which can be divided into two major approaches: (1) non-covalent supramolecular modifications, and (2) covalent functionalisation. In this tutorial review, we survey the covalent modification of SWCNTs with organic moieties, and illustrate the major analytical techniques routinely used to characterise the functionalised materials.},
keywords = {Alkylation, carbon, Esterification, Free Radicals, Halogenation, I2CT, Microscopy, Nanotubes, Oxidation-Reduction, Spectrum Analysis, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Herrero Antonia M, Toma Francesca M, Al-Jamal Khuloud T, Kostarelos Kostas, Bianco Alberto, Ros Tatiana Da, Bano Fouzia, Casalis Loredana, Scoles Giacinto, Prato Maurizio
Synthesis and characterization of a carbon nanotube-dendron series for efficient siRNA delivery Article de journal
Dans: Journal of the American Chemical Society, vol. 131, no. 28, p. 9843–9848, 2009, ISSN: 1520-5126.
Résumé | Liens | BibTeX | Étiquettes: Acrylates, Animals, Azo Compounds, Biological Transport, carbon, Cytoplasm, Dendrimers, Drug Carriers, Ethylenediamines, Gene Silencing, HeLa Cells, Humans, I2CT, Nanotubes, Polyamines, RNA, Small Interfering, Solubility, Team-Bianco, Thiosemicarbazones, Transfection, water
@article{herrero_synthesis_2009,
title = {Synthesis and characterization of a carbon nanotube-dendron series for efficient siRNA delivery},
author = {Antonia M Herrero and Francesca M Toma and Khuloud T Al-Jamal and Kostas Kostarelos and Alberto Bianco and Tatiana Da Ros and Fouzia Bano and Loredana Casalis and Giacinto Scoles and Maurizio Prato},
doi = {10.1021/ja903316z},
issn = {1520-5126},
year = {2009},
date = {2009-07-01},
journal = {Journal of the American Chemical Society},
volume = {131},
number = {28},
pages = {9843--9848},
abstract = {A new series of dendron-functionalized multiwalled carbon nanotube (MWNT) derivatives, characterized by the presence of numerous positively charged tetraalkyl ammonium salts at the periphery of the dendron, has been synthesized. The positive charges on the MWNT surface, coupled with the unique ability of carbon nanotubes (CNTs) to penetrate cell membranes, make the new derivatives potentially ideal vectors for siRNA delivery. Using a fluorescently labeled, noncoding siRNA sequence, we demonstrate that cytoplasmic delivery of the nucleic acid is remarkably increased throughout the different dendron generations. The work reported here highlights the fact that dendron-functionalized CNTs can be rationally designed as efficient carriers of siRNA that can eventually lead to gene silencing.},
keywords = {Acrylates, Animals, Azo Compounds, Biological Transport, carbon, Cytoplasm, Dendrimers, Drug Carriers, Ethylenediamines, Gene Silencing, HeLa Cells, Humans, I2CT, Nanotubes, Polyamines, RNA, Small Interfering, Solubility, Team-Bianco, Thiosemicarbazones, Transfection, water},
pubstate = {published},
tppubtype = {article}
}
Marega Riccardo, Aroulmoji Vincent, Dinon Francesca, Vaccari Lisa, Giordani Silvia, Bianco Alberto, Murano Erminio, Prato Maurizio
Diffusion-ordered NMR spectroscopy in the structural characterization of functionalized carbon nanotubes Article de journal
Dans: Journal of the American Chemical Society, vol. 131, no. 25, p. 9086–9093, 2009, ISSN: 1520-5126.
Résumé | Liens | BibTeX | Étiquettes: carbon, Diffusion, I2CT, Magnetic Resonance Spectroscopy, Nanotubes, Oxidation-Reduction, Surface Properties, Team-Bianco
@article{marega_diffusion-ordered_2009,
title = {Diffusion-ordered NMR spectroscopy in the structural characterization of functionalized carbon nanotubes},
author = {Riccardo Marega and Vincent Aroulmoji and Francesca Dinon and Lisa Vaccari and Silvia Giordani and Alberto Bianco and Erminio Murano and Maurizio Prato},
doi = {10.1021/ja902728w},
issn = {1520-5126},
year = {2009},
date = {2009-07-01},
journal = {Journal of the American Chemical Society},
volume = {131},
number = {25},
pages = {9086--9093},
abstract = {The emerging applications of functionalized carbon nanotubes (CNTs) in various research domains necessitate the use of many different analytical techniques to confirm their structural modifications in a fast and reliable manner. Thus far, NMR spectroscopy has not been among the main tools for characterization of organically modified carbon nanostructures. (1)H analysis is limited because the signals in these derivatives are typically weak and broad, resulting in uncertainties of a few parts per million, and because of the strong interference of residual solvent signals. To overcome these limitations, we investigated the applicability of proton NMR spectroscopy based on gradient-edited diffusion pulse sequences (1D diffusion-ordered spectroscopy, DOSY) in the characterization of CNT derivatives. In general, diffusion NMR experiments allow the separation of NMR signals of different species present in a mixture, according to their own diffusion coefficients, merging spectroscopy information with size analysis. In the present study, a selected set of CNT derivatives was synthesized and analyzed using 1D DOSY experiments by applying strong magnetic field gradients (up to 42.6 G cm(-1)). Colorimetric tests (i.e., Kaiser test) and TGA analysis support the NMR findings, which are related to isolated and/or bundled short SWNTs, on the basis of TEM and AFM characterization. The overall results show that the diffusion-based NMR spectroscopy is a fast and promising approach for the characterization of covalently modified CNT derivatives.},
keywords = {carbon, Diffusion, I2CT, Magnetic Resonance Spectroscopy, Nanotubes, Oxidation-Reduction, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Schett G, Dumortier H, Hoefler E, Muller S, Steiner G
B cell epitopes of the heterogeneous nuclear ribonucleoprotein A2: identification of a new specific antibody marker for active lupus disease Article de journal
Dans: Annals of the Rheumatic Diseases, vol. 68, no. 5, p. 729–735, 2009, ISSN: 1468-2060.
Résumé | Liens | BibTeX | Étiquettes: Autoantibodies, B-Lymphocyte, Biomarkers, Dumortier, Enzyme-Linked Immunosorbent Assay, Epitopes, Female, Follow-Up Studies, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, Humans, I2CT, Lupus Erythematosus, Male, Rheumatic Diseases, Severity of Illness Index, Systemic, Team-Dumortier
@article{schett_b_2009,
title = {B cell epitopes of the heterogeneous nuclear ribonucleoprotein A2: identification of a new specific antibody marker for active lupus disease},
author = {G Schett and H Dumortier and E Hoefler and S Muller and G Steiner},
doi = {10.1136/ard.2007.087502},
issn = {1468-2060},
year = {2009},
date = {2009-05-01},
journal = {Annals of the Rheumatic Diseases},
volume = {68},
number = {5},
pages = {729--735},
abstract = {OBJECTIVES: Autoantibody formation and T cell reactivity against the heterogeneous nuclear ribonucleoprotein A2 (hnRNP-A2) has been observed in patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). Since no differences in epitope recognition were reported and the usefulness of anti-hnRNP-A2 antibodies as diagnostic markers of SLE is unknown, it was our objective to characterise linear B cell epitopes of hnRNP-A2 and to relate the anti-hnRNP-A2 antibody responses to disease activity and clinical features of SLE.
METHODS: Sequential serum samples from 15 patients with SLE and sera from patients with other rheumatic diseases and healthy subjects were investigated by ELISA for autoantibody reactivities against a set of 13 overlapping peptides spanning the RNA-binding region of hnRNP-A2. Antibody reactivity against the complete protein was determined by western immunoblotting and ELISA. SLE disease activity was assessed by European Consensus Lupus Activity Measure scores, by SLE Index scores and the British Isles Lupus Assessment index.
RESULTS: Anti-peptide antibody reactivities were found in 60% of SLE sera but in only 5% of control samples, and were mainly directed to four peptides, one of which (p155-175) appeared to be immunodominant. Antibodies to p155-175 were exclusively seen in patients with SLE and correlated with clinical disease activity as well as kidney and skin involvement. No correlations were found for the other anti-peptide antibody responses.
CONCLUSION: Peptide p155-175 encompasses a disease-specific immunodominant epitope of hnRNP-A2. Since antibodies to p155-175 correlate with disease activity and nephritis, they may be useful as markers for active SLE.},
keywords = {Autoantibodies, B-Lymphocyte, Biomarkers, Dumortier, Enzyme-Linked Immunosorbent Assay, Epitopes, Female, Follow-Up Studies, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, Humans, I2CT, Lupus Erythematosus, Male, Rheumatic Diseases, Severity of Illness Index, Systemic, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}
Podesta Jennifer E, Al-Jamal Khuloud T, Herrero Antonia M, Tian Bowen, Ali-Boucetta Hanene, Hegde Vikas, Bianco Alberto, Prato Maurizio, Kostarelos Kostas
Antitumor activity and prolonged survival by carbon-nanotube-mediated therapeutic siRNA silencing in a human lung xenograft model Article de journal
Dans: Small (Weinheim an Der Bergstrasse, Germany), vol. 5, no. 10, p. 1176–1185, 2009, ISSN: 1613-6829.
Résumé | Liens | BibTeX | Étiquettes: Animals, Antineoplastic Agents, Apoptosis, carbon, Cell Line, Cell Proliferation, Electrophoresis, Gene Silencing, Humans, I2CT, Liposomes, Lung Neoplasms, Mice, Nanomedicine, Nanotubes, RNA, Small Interfering, Survival Analysis, Team-Bianco, tumor, Xenograft Model Antitumor Assays
@article{podesta_antitumor_2009,
title = {Antitumor activity and prolonged survival by carbon-nanotube-mediated therapeutic siRNA silencing in a human lung xenograft model},
author = {Jennifer E Podesta and Khuloud T Al-Jamal and Antonia M Herrero and Bowen Tian and Hanene Ali-Boucetta and Vikas Hegde and Alberto Bianco and Maurizio Prato and Kostas Kostarelos},
doi = {10.1002/smll.200801572},
issn = {1613-6829},
year = {2009},
date = {2009-05-01},
journal = {Small (Weinheim an Der Bergstrasse, Germany)},
volume = {5},
number = {10},
pages = {1176--1185},
abstract = {Carbon nanotubes are novel nanomaterials that are thought to offer potential benefits to a variety of biomedical and clinical applications. In this study, the treatment of a human lung carcinoma model in vivo using siRNA sequences leading to cytotoxicity and cell death is carried out using either cationic liposomes (DOTAP:cholesterol) or amino-functionalized multi-walled carbon nanotubes (MWNT - NH(+)(3)). Validation for the most cytotoxic siRNA sequence using a panel of human carcinoma and murine cells reveals that the proprietary siTOX sequence is human specific and can lead to significant cytotoxic activities delivered both by liposome or MWNT - NH(+)(3) in vitro. A comparative study using both types of vector indicates that only MWNT - NH(+)(3):siRNA complexes administered intratumorally can elicit delayed tumor growth and increased survival of xenograft-bearing animals. siTOX delivery via the cationic MWNT - NH(+)(3) is biologically active in vivo by triggering an apoptotic cascade, leading to extensive necrosis of the human tumor mass. This suggests that carbon-nanotube-mediated delivery of siRNA by intratumoral administration leads to successful and statistically significant suppression of tumor volume, followed by a concomitant prolongation of survival of human lung tumor-bearing animals. The direct comparison between carbon nanotubes and liposomes demonstrates the potential advantages offered by carbon nanotubes for the intracellular delivery of therapeutic agents in vivo. The present work may act as the impetus for further studies to explore the therapeutic capacity of chemically functionalized carbon nanotubes to deliver siRNA directly into the cytoplasm of target cells and achieve effective therapeutic silencing in various disease indications where local delivery is feasible or desirable.},
keywords = {Animals, Antineoplastic Agents, Apoptosis, carbon, Cell Line, Cell Proliferation, Electrophoresis, Gene Silencing, Humans, I2CT, Liposomes, Lung Neoplasms, Mice, Nanomedicine, Nanotubes, RNA, Small Interfering, Survival Analysis, Team-Bianco, tumor, Xenograft Model Antitumor Assays},
pubstate = {published},
tppubtype = {article}
}
Bianco Alberto
Potential usefulness of carbon nanotubes for cancer therapy Article de journal
Dans: Medecine Sciences: M/S, vol. 25, no. 2, p. 125–127, 2009, ISSN: 0767-0974.
Liens | BibTeX | Étiquettes: carbon, Graphite, Humans, I2CT, Nanotubes, Neoplasms, Team-Bianco
@article{bianco_potential_2009,
title = {Potential usefulness of carbon nanotubes for cancer therapy},
author = {Alberto Bianco},
doi = {10.1051/medsci/2009252125},
issn = {0767-0974},
year = {2009},
date = {2009-02-01},
journal = {Medecine Sciences: M/S},
volume = {25},
number = {2},
pages = {125--127},
keywords = {carbon, Graphite, Humans, I2CT, Nanotubes, Neoplasms, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Fauny Jean-Daniel, Thisse Bernard, Thisse Christine
The entire zebrafish blastula-gastrula margin acts as an organizer dependent on the ratio of Nodal to BMP activity Article de journal
Dans: Development (Cambridge, England), vol. 136, no. 22, p. 3811–3819, 2009, ISSN: 1477-9129.
Résumé | Liens | BibTeX | Étiquettes: Animals, Blastula, Bone Morphogenetic Proteins, Embryonic, Gastrula, I2CT, Imagerie, Nodal Protein, Organizers, Zebrafish, Zebrafish Proteins
@article{fauny_entire_2009,
title = {The entire zebrafish blastula-gastrula margin acts as an organizer dependent on the ratio of Nodal to BMP activity},
author = {Jean-Daniel Fauny and Bernard Thisse and Christine Thisse},
url = {http://www.ncbi.nlm.nih.gov/pubmed/19855023},
doi = {10.1242/dev.039693},
issn = {1477-9129},
year = {2009},
date = {2009-01-01},
urldate = {2011-10-24},
journal = {Development (Cambridge, England)},
volume = {136},
number = {22},
pages = {3811--3819},
abstract = {Formation of the vertebrate embryo is known to depend on the activity of organizing centers. The dorsal Spemann organizer is the source of growth factor antagonists that participate in the creation of signaling gradients. In various species, the existence of head, trunk and trunk-tail inducers has been proposed to explain the formation of different parts of the embryo along the anteroposterior (A/P) axis. In zebrafish, two organizing centers have been described, the dorsal and tail organizers, located at the dorsal and ventral gastrula margins, respectively. Here, we report that organizer functions are executed not only by the dorsal and ventral margins, but also by all parts of the blastula-gastrula margin. The position of different marginal territories along the dorsoventral axis defines the A/P nature of the structures they are able to organize. At the molecular level, we show that this organizing activity results from the simultaneous activation of BMP and Nodal signaling pathways. Furthermore, the A/P character of the organized structures is not defined by absolute levels but instead by the ratio of BMP and Nodal activities. Rather than resulting from the activity of discrete centers, organization of the zebrafish embryo depends on the activity of the entire margin acting as a continuous and global organizer that is established by a gradual ventral-to-dorsal modulation of the ratio of marginal BMP to Nodal activity.},
keywords = {Animals, Blastula, Bone Morphogenetic Proteins, Embryonic, Gastrula, I2CT, Imagerie, Nodal Protein, Organizers, Zebrafish, Zebrafish Proteins},
pubstate = {published},
tppubtype = {article}
}
Monneaux Fanny, Muller Sylviane
Molecular therapies for systemic lupus erythematosus: clinical trials and future prospects Article de journal
Dans: Arthritis Research & Therapy, vol. 11, no. 3, p. 234, 2009, ISSN: 1478-6362.
Résumé | Liens | BibTeX | Étiquettes: Animals, Clinical Trials as Topic, Forecasting, Genetic Therapy, Humans, I2CT, Lupus Erythematosus, Monneaux, Systemic, Team-Dumortier
@article{monneaux_molecular_2009,
title = {Molecular therapies for systemic lupus erythematosus: clinical trials and future prospects},
author = {Fanny Monneaux and Sylviane Muller},
doi = {10.1186/ar2711},
issn = {1478-6362},
year = {2009},
date = {2009-01-01},
journal = {Arthritis Research & Therapy},
volume = {11},
number = {3},
pages = {234},
abstract = {The prognosis of patients with systemic lupus erythematosus has greatly improved since treatment regimens combining corticosteroids and immunosuppressive medications have been widely adopted in therapeutic strategies given to these patients. Immune suppression is evidently efficient but also leads to higher susceptibility to infectious and malignant diseases. Toxic effects and sometimes unexpectedly dramatic complications of current therapies have been progressively reported. Identifying novel molecular targets therefore remains an important issue in the treatment of lupus. The aim of this review article is to highlight emerging pharmacological options and new therapeutic avenues for lupus with a particular focus on non-antibody molecular strategies.},
keywords = {Animals, Clinical Trials as Topic, Forecasting, Genetic Therapy, Humans, I2CT, Lupus Erythematosus, Monneaux, Systemic, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}
Lacotte Stéphanie, Brun Susana, Muller Sylviane, Dumortier Hélène
CXCR3, inflammation, and autoimmune diseases Article de journal
Dans: Annals of the New York Academy of Sciences, vol. 1173, p. 310–317, 2009, ISSN: 1749-6632.
Résumé | Liens | BibTeX | Étiquettes: Animals, arthritis, Biological, Chemokine CXCL10, Chemokine CXCL11, Chemokine CXCL9, CXCR3, Dumortier, Humans, I2CT, inflammation, Lupus Erythematosus, Models, Receptors, rheumatoid, Systemic, Team-Dumortier
@article{lacotte_cxcr3_2009,
title = {CXCR3, inflammation, and autoimmune diseases},
author = {Stéphanie Lacotte and Susana Brun and Sylviane Muller and Hélène Dumortier},
doi = {10.1111/j.1749-6632.2009.04813.x},
issn = {1749-6632},
year = {2009},
date = {2009-01-01},
journal = {Annals of the New York Academy of Sciences},
volume = {1173},
pages = {310--317},
abstract = {CXCR3 is a G protein-coupled, seven-transmembrane receptor that binds and is activated by the three IFN-gamma-inducible chemokines of the CXC family named CXCL9, CXCL10, and CXCL11. These chemokines are not constitutively expressed but are up-regulated in a proinflammatory cytokine milieu. Consequently, their major function is to selectively recruit immune cells at inflammation sites, but they also play a role in angiogenesis mechanisms. In the last few years, strong experimental and clinical evidence has been obtained supporting the idea that the CXCR3 pathway is involved in the development of autoimmune diseases, especially by creating local amplification loops of inflammation in target organs, thereby inducing worsening of clinical manifestations. This article briefly reviews what we know today about the nature and functions of CXCR3, with special emphasis on its involvement in two main rheumatic systemic autoimmune diseases, namely rheumatoid arthritis and systemic lupus erythematosus.},
keywords = {Animals, arthritis, Biological, Chemokine CXCL10, Chemokine CXCL11, Chemokine CXCL9, CXCR3, Dumortier, Humans, I2CT, inflammation, Lupus Erythematosus, Models, Receptors, rheumatoid, Systemic, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}
Gaillard Claire, Cellot Giada, Li Shouping, Toma Francesca Maria, Dumortier Hélène, Spalluto Giampiero, Cacciari Barbara, Prato Maurizio, Ballerini Laura, Bianco Alberto
Carbon Nanotubes Carrying Cell-Adhesion Peptides do not Interfere with Neuronal Functionality Article de journal
Dans: Advanced Materials, vol. 21, no. 28, p. 2903–2908, 2009, ISSN: 1521-4095.
Résumé | Liens | BibTeX | Étiquettes: Carbon nanotubes, Cytotoxicity, I2CT, mammalian cells, Neurons, Peptides, Team-Bianco
@article{gaillard_carbon_2009,
title = {Carbon Nanotubes Carrying Cell-Adhesion Peptides do not Interfere with Neuronal Functionality},
author = {Claire Gaillard and Giada Cellot and Shouping Li and Francesca Maria Toma and Hélène Dumortier and Giampiero Spalluto and Barbara Cacciari and Maurizio Prato and Laura Ballerini and Alberto Bianco},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.200900050},
doi = {10.1002/adma.200900050},
issn = {1521-4095},
year = {2009},
date = {2009-01-01},
urldate = {2020-03-31},
journal = {Advanced Materials},
volume = {21},
number = {28},
pages = {2903--2908},
abstract = {Water-soluble carbon nanotubes functionalized with cell-adhesion peptides do not affect the viability of different cell types, including Jurkat cells, splenocytes, and neurons. They also do not modify the neuronal morphology and basic functions, thus representing a promising candidate for the exploitation of novel drug-delivery systems or for designing a new generation of self-assembling nerve bridges.},
keywords = {Carbon nanotubes, Cytotoxicity, I2CT, mammalian cells, Neurons, Peptides, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Bianco Alberto
Les nanotubes de carbone : un nouvel outil contre le cancer Article de journal
Dans: M/S. Médecine sciences [ISSN papier : 0767-0974 ; ISSN numérique : 1958-5381], 2009, Vol. 25, N° 2; p. 125-127, 2009, ISSN: 1958-5381.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{bianco_les_2009,
title = {Les nanotubes de carbone : un nouvel outil contre le cancer},
author = {Alberto Bianco},
url = {http://www.ipubli.inserm.fr/handle/10608/6569},
doi = {10.1051/medsci/2009252125},
issn = {1958-5381},
year = {2009},
date = {2009-01-01},
urldate = {2020-04-01},
journal = {M/S. Médecine sciences [ISSN papier : 0767-0974 ; ISSN numérique : 1958-5381], 2009, Vol. 25, N° 2; p. 125-127},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Guichard Gilles, Trouche Nathalie, Wieckowski Sébastien, Sun Weimin, Chaloin Olivier, Bianco Alberto, Hoebeke Johan, Schneider Pascal, Fournel Sylvie
Rationally-designed multivalent architectures for mimicking homotrimers of CD40L, a member of the TNF superfamily Article de journal
Dans: Advances in Experimental Medicine and Biology, vol. 611, p. 355–357, 2009, ISSN: 0065-2598.
Liens | BibTeX | Étiquettes: Biopolymers, CD40 Ligand, I2CT, Models, Molecular, Molecular Mimicry, Team-Bianco, X-Ray Diffraction
@article{guichard_rationally-designed_2009,
title = {Rationally-designed multivalent architectures for mimicking homotrimers of CD40L, a member of the TNF superfamily},
author = {Gilles Guichard and Nathalie Trouche and Sébastien Wieckowski and Weimin Sun and Olivier Chaloin and Alberto Bianco and Johan Hoebeke and Pascal Schneider and Sylvie Fournel},
doi = {10.1007/978-0-387-73657-0_157},
issn = {0065-2598},
year = {2009},
date = {2009-01-01},
journal = {Advances in Experimental Medicine and Biology},
volume = {611},
pages = {355--357},
keywords = {Biopolymers, CD40 Ligand, I2CT, Models, Molecular, Molecular Mimicry, Team-Bianco, X-Ray Diffraction},
pubstate = {published},
tppubtype = {article}
}
Geotti-Bianchini Piero, Crisma Marco, Peggion Cristina, Bianco Alberto, Formaggio Fernando
Conformationally controlled, thymine-based alpha-nucleopeptides Article de journal
Dans: Chemical Communications (Cambridge, England), no. 22, p. 3178–3180, 2009, ISSN: 1359-7345.
Résumé | Liens | BibTeX | Étiquettes: I2CT, Magnetic Resonance Spectroscopy, Oligopeptides, Protein Conformation, Team-Bianco, thymine, X-Ray Diffraction
@article{geotti-bianchini_conformationally_2009,
title = {Conformationally controlled, thymine-based alpha-nucleopeptides},
author = {Piero Geotti-Bianchini and Marco Crisma and Cristina Peggion and Alberto Bianco and Fernando Formaggio},
doi = {10.1039/b822789f},
issn = {1359-7345},
year = {2009},
date = {2009-01-01},
journal = {Chemical Communications (Cambridge, England)},
number = {22},
pages = {3178--3180},
abstract = {Rigid peptide backbones and backbone-to-side chain H-bonds permit the design of alpha-nucleopeptides with known 3D-structure; thymine-thymine base pairing is also observed.},
keywords = {I2CT, Magnetic Resonance Spectroscopy, Oligopeptides, Protein Conformation, Team-Bianco, thymine, X-Ray Diffraction},
pubstate = {published},
tppubtype = {article}
}
Geotti-Bianchini Piero, Crisma Marco, Peggion Cristina, Bianco Alberto, Formaggio Fernando
Synthesis and 3D-structure of conformationally controlled nucleo-peptides Article de journal
Dans: Advances in Experimental Medicine and Biology, vol. 611, p. 37–38, 2009, ISSN: 0065-2598.
Liens | BibTeX | Étiquettes: I2CT, Models, Molecular, Nucleoproteins, Peptides, Protein Conformation, Team-Bianco
@article{geotti-bianchini_synthesis_2009,
title = {Synthesis and 3D-structure of conformationally controlled nucleo-peptides},
author = {Piero Geotti-Bianchini and Marco Crisma and Cristina Peggion and Alberto Bianco and Fernando Formaggio},
doi = {10.1007/978-0-387-73657-0_16},
issn = {0065-2598},
year = {2009},
date = {2009-01-01},
journal = {Advances in Experimental Medicine and Biology},
volume = {611},
pages = {37--38},
keywords = {I2CT, Models, Molecular, Nucleoproteins, Peptides, Protein Conformation, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Yandar Nubia, Pastorin Giorgia, Prato Maurizio, Bianco Alberto, Patarroyo Manuel Elkin, Lozano José Manuel
Immunological profile of a Plasmodium vivax AMA-1 N-terminus peptide-carbon nanotube conjugate in an infected Plasmodium berghei mouse model Article de journal
Dans: Vaccine, vol. 26, no. 46, p. 5864–5873, 2008, ISSN: 0264-410X.
Résumé | Liens | BibTeX | Étiquettes: Apical membrane antigen 1 (AMA-1), Functionalized carbon nanotubes (-CNT), I2CT, Synthetic vaccine delivery system, Team-Bianco
@article{yandar_immunological_2008,
title = {Immunological profile of a Plasmodium vivax AMA-1 N-terminus peptide-carbon nanotube conjugate in an infected Plasmodium berghei mouse model},
author = {Nubia Yandar and Giorgia Pastorin and Maurizio Prato and Alberto Bianco and Manuel Elkin Patarroyo and José Manuel Lozano},
url = {http://www.sciencedirect.com/science/article/pii/S0264410X08010736},
doi = {10.1016/j.vaccine.2008.08.014},
issn = {0264-410X},
year = {2008},
date = {2008-10-01},
urldate = {2020-03-31},
journal = {Vaccine},
volume = {26},
number = {46},
pages = {5864--5873},
abstract = {We have covalently conjugated an N-terminus Plasmodium vivax apical membrane antigen-1 (AMA-1) peptide to functionalized carbon nanotubes (f-CNT). Immunological characterization of this molecular conjugate revealed that the immunogen-AMA-1 peptide was appropriately presented after being conjugated to CNTs as well as being recognized by BALB/c polyclonal antibodies. Subsequent experiments lead us to assess the AMA-1 peptide alone, as well as the CNT-peptide conjugate regarding rodent malarial infection. Remarkably, the peptide effectively controlled and delayed Plasmodium berghei-challenged animals’ parasitaemia. The peptide-CNT conjugate displayed similar immunological properties to the peptide alone by protecting or delaying malarial infection. The peptide presentation by f-CNT to the immune system thus constitutes a promising approach for synthetic malarial vaccine formulation since the immunogen peptide conformation is well preserved.},
keywords = {Apical membrane antigen 1 (AMA-1), Functionalized carbon nanotubes (-CNT), I2CT, Synthetic vaccine delivery system, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Geotti-Bianchini Piero, Beyrath Julien, Chaloin Olivier, Formaggio Fernando, Bianco Alberto
Design and synthesis of intrinsically cell-penetrating nucleopeptides Article de journal
Dans: Organic & Biomolecular Chemistry, vol. 6, no. 20, p. 3661–3663, 2008, ISSN: 1477-0539.
Résumé | Liens | BibTeX | Étiquettes: Amino Acid Sequence, Cell Line, Cells, Drug Design, Humans, I2CT, Molecular Sequence Data, Peptides, Purines, Pyrimidines, Team-Bianco
@article{geotti-bianchini_design_2008,
title = {Design and synthesis of intrinsically cell-penetrating nucleopeptides},
author = {Piero Geotti-Bianchini and Julien Beyrath and Olivier Chaloin and Fernando Formaggio and Alberto Bianco},
doi = {10.1039/b811639c},
issn = {1477-0539},
year = {2008},
date = {2008-10-01},
journal = {Organic & Biomolecular Chemistry},
volume = {6},
number = {20},
pages = {3661--3663},
abstract = {Nucleopeptides, which are constituted of alpha-amino acids bearing nucleobases at their side chains, are able to penetrate into cells and to reach the nucleus without cytotoxic effects.},
keywords = {Amino Acid Sequence, Cell Line, Cells, Drug Design, Humans, I2CT, Molecular Sequence Data, Peptides, Purines, Pyrimidines, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Lacerda Lara, Herrero Maria A, Venner Kerrie, Bianco Alberto, Prato Maurizio, Kostarelos Kostas
Carbon-nanotube shape and individualization critical for renal excretion Article de journal
Dans: Small (Weinheim an Der Bergstrasse, Germany), vol. 4, no. 8, p. 1130–1132, 2008, ISSN: 1613-6829.
Liens | BibTeX | Étiquettes: Animals, Biological Transport, carbon, Electron, Female, Glomerular Filtration Rate, I2CT, Inbred BALB C, Kidney Glomerulus, Mice, Microscopy, Nanoparticles, nanotechnology, Nanotubes, Team-Bianco, Transmission
@article{lacerda_carbon-nanotube_2008,
title = {Carbon-nanotube shape and individualization critical for renal excretion},
author = {Lara Lacerda and Maria A Herrero and Kerrie Venner and Alberto Bianco and Maurizio Prato and Kostas Kostarelos},
doi = {10.1002/smll.200800323},
issn = {1613-6829},
year = {2008},
date = {2008-08-01},
journal = {Small (Weinheim an Der Bergstrasse, Germany)},
volume = {4},
number = {8},
pages = {1130--1132},
keywords = {Animals, Biological Transport, carbon, Electron, Female, Glomerular Filtration Rate, I2CT, Inbred BALB C, Kidney Glomerulus, Mice, Microscopy, Nanoparticles, nanotechnology, Nanotubes, Team-Bianco, Transmission},
pubstate = {published},
tppubtype = {article}
}
Parietti Véronique, Monneaux Fanny, Décossas Marion, Muller Sylviane
Function of CD4+,CD25+ Treg cells in MRL/lpr mice is compromised by intrinsic defects in antigen-presenting cells and effector Ŧ cells Article de journal
Dans: Arthritis and Rheumatism, vol. 58, no. 6, p. 1751–1761, 2008, ISSN: 0004-3591.
Résumé | Liens | BibTeX | Étiquettes: Animal, Animals, Antigen-Presenting Cells, Antigens, B7-1 Antigen, B7-2 Antigen, CD, Cell Communication, Cells, Coculture Techniques, CTLA-4 Antigen, Cultured, Disease Models, Female, I2CT, Interleukin-1, Interleukin-2 Receptor alpha Subunit, Lupus Erythematosus, Mice, Monneaux, Regulatory, Systemic, T-Lymphocyte Subsets, T-Lymphocytes, Team-Dumortier
@article{parietti_function_2008,
title = {Function of CD4+,CD25+ Treg cells in MRL/lpr mice is compromised by intrinsic defects in antigen-presenting cells and effector Ŧ cells},
author = {Véronique Parietti and Fanny Monneaux and Marion Décossas and Sylviane Muller},
doi = {10.1002/art.23464},
issn = {0004-3591},
year = {2008},
date = {2008-06-01},
journal = {Arthritis and Rheumatism},
volume = {58},
number = {6},
pages = {1751--1761},
abstract = {OBJECTIVE: Naturally occurring CD4+,CD25+ Treg cells are central in the maintenance of peripheral tolerance. Impaired activity and/or a lower frequency of these cells is involved in the emergence of autoimmunity. We undertook this study to analyze relative proportions and functional alterations of Treg cells in MRL/lpr mice.
METHODS: The frequency of CD4+,CD25+ T cells in the peripheral blood of healthy and autoimmune mice was compared by flow cytometry. The capacity of CD4+,CD25+ T cells to inhibit the proliferation and cytokine secretion of CD4+,CD25- T cells was assessed after polyclonal activation.
RESULTS: MRL/lpr mice exhibited a normal percentage of CD4+,CD25 high T cells, and forkhead box P3 messenger RNA and protein expression in Treg cells was not altered. However, MRL/lpr Treg cells displayed a reduced capacity to suppress proliferation and to inhibit interferon-gamma secretion by syngeneic effector CD4+,CD25- T cells, as compared with syngeneic cocultures of CBA/J T cells. Moreover, effector MRL/lpr CD4+,CD25- T cells were substantially less susceptible to suppression even when cultured with CBA/J or MRL/lpr Treg cells. Crossover experiments led us to conclude that in MRL/lpr mice, each partner engaged in T cell regulation displays altered functions. Molecules involved in suppressive mechanisms (CTLA-4 and CD80/CD86) are underexpressed, and antigen-presenting cells (APCs) produce raised levels of interleukin-6, which is known to abrogate suppression.
CONCLUSION: Our results suggest that although the frequency and phenotype of Treg cells in MRL/lpr mice are similar to those in normal mice, Treg cells in MRL/lpr mice are not properly stimulated by APCs and are unable to suppress proinflammatory cytokine secretion from effector T cells.},
keywords = {Animal, Animals, Antigen-Presenting Cells, Antigens, B7-1 Antigen, B7-2 Antigen, CD, Cell Communication, Cells, Coculture Techniques, CTLA-4 Antigen, Cultured, Disease Models, Female, I2CT, Interleukin-1, Interleukin-2 Receptor alpha Subunit, Lupus Erythematosus, Mice, Monneaux, Regulatory, Systemic, T-Lymphocyte Subsets, T-Lymphocytes, Team-Dumortier},
pubstate = {published},
tppubtype = {article}
}
Fabre Bruno, Hauquier Fanny, Herrier Cyril, Pastorin Giorgia, Wu Wei, Bianco Alberto, Prato Maurizio, Hapiot Philippe, Zigah Dodzi, Prasciolu Mauro, Vaccari Lisa
Covalent assembly and micropatterning of functionalized multiwalled carbon nanotubes to monolayer-modified Si(111) surfaces Article de journal
Dans: Langmuir: the ACS journal of surfaces and colloids, vol. 24, no. 13, p. 6595–6602, 2008, ISSN: 0743-7463.
Résumé | Liens | BibTeX | Étiquettes: Atomic Force, carbon, Electrochemistry, Electron, I2CT, Microscopy, Nanotubes, scanning, Silicon, Surface Properties, Team-Bianco
@article{fabre_covalent_2008,
title = {Covalent assembly and micropatterning of functionalized multiwalled carbon nanotubes to monolayer-modified Si(111) surfaces},
author = {Bruno Fabre and Fanny Hauquier and Cyril Herrier and Giorgia Pastorin and Wei Wu and Alberto Bianco and Maurizio Prato and Philippe Hapiot and Dodzi Zigah and Mauro Prasciolu and Lisa Vaccari},
doi = {10.1021/la800358w},
issn = {0743-7463},
year = {2008},
date = {2008-06-01},
journal = {Langmuir: the ACS journal of surfaces and colloids},
volume = {24},
number = {13},
pages = {6595--6602},
abstract = {Multiwalled carbon nanotubes (MWNTs) covalently bound to monocrystalline p-type Si(111) surfaces have been prepared by attaching soluble amine-functionalized MWNTs onto a preassembled undecanoic acid monolayer using carbodiimide coupling. SEM analysis of these functionalized surfaces shows that the bound MWNTs are parallel to the surface rather than perpendicular. The voltammetric and electrochemical impedance spectroscopy measurements reveal that the electron transfer at the MWNT-modified surface is faster than that observed at a MWNT-free alkyl monolayer. We have also demonstrated that it is possible to prepare MWNT micropatterns using this surface amidation reaction and a "reagentless" UV photolithography technique. Following this approach, MWNT patterns surrounded by n-dodecyl areas have been produced and the local electrochemical properties of these micropatterned surfaces have been examined by scanning electrochemical microscopy. In particular, it is demonstrated that the MWNT patterns allow a faster charge transfer which is consistent with the results obtained for the uniformly modified surfaces.},
keywords = {Atomic Force, carbon, Electrochemistry, Electron, I2CT, Microscopy, Nanotubes, scanning, Silicon, Surface Properties, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Li Shouping, Wu Wei, Campidelli Stéphane, Sarnatskaïa Veronika, Prato Maurizio, Tridon Arlette, Nikolaev Andrey, Nikolaev Vladimir, Bianco Alberto, Snezhkova Elisaveta
Adsorption of carbon nanotubes on active carbon microparticles Article de journal
Dans: Carbon, vol. 46, no. 7, p. 1091–1095, 2008, ISSN: 0008-6223.
Liens | BibTeX | Étiquettes: I2CT, Team-Bianco
@article{li_adsorption_2008,
title = {Adsorption of carbon nanotubes on active carbon microparticles},
author = {Shouping Li and Wei Wu and Stéphane Campidelli and Veronika Sarnatskaïa and Maurizio Prato and Arlette Tridon and Andrey Nikolaev and Vladimir Nikolaev and Alberto Bianco and Elisaveta Snezhkova},
url = {http://www.sciencedirect.com/science/article/pii/S0008622308001462},
doi = {10.1016/j.carbon.2008.03.010},
issn = {0008-6223},
year = {2008},
date = {2008-06-01},
urldate = {2020-03-31},
journal = {Carbon},
volume = {46},
number = {7},
pages = {1091--1095},
keywords = {I2CT, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Kostarelos Kostas, Bianco Alberto, Prato Maurizio
Hype around nanotubes creates unrealistic hopes Article de journal
Dans: Nature, vol. 453, no. 7193, p. 280, 2008, ISSN: 1476-4687.
Liens | BibTeX | Étiquettes: Adult Stem Cells, carbon, Humans, I2CT, Nanomedicine, Nanotubes, Reproducibility of Results, Team-Bianco
@article{kostarelos_hype_2008,
title = {Hype around nanotubes creates unrealistic hopes},
author = {Kostas Kostarelos and Alberto Bianco and Maurizio Prato},
doi = {10.1038/453280c},
issn = {1476-4687},
year = {2008},
date = {2008-05-01},
journal = {Nature},
volume = {453},
number = {7193},
pages = {280},
keywords = {Adult Stem Cells, carbon, Humans, I2CT, Nanomedicine, Nanotubes, Reproducibility of Results, Team-Bianco},
pubstate = {published},
tppubtype = {article}
}
Lacerda Lara, Ali-Boucetta Hanene, Herrero Maria A, Pastorin Giorgia, Bianco Alberto, Prato Maurizio, Kostarelos Kostas
Tissue histology and physiology following intravenous administration of different types of functionalized multiwalled carbon nanotubes Article de journal
Dans: Nanomedicine (London, England), vol. 3, no. 2, p. 149–161, 2008, ISSN: 1748-6963.
Résumé | Liens | BibTeX | Étiquettes: Animals, carbon, Female, I2CT, Inbred BALB C, Injections, Intravenous, Mice, Nanotubes, Organ Specificity, Team-Bianco, Tissue Distribution
@article{lacerda_tissue_2008,
title = {Tissue histology and physiology following intravenous administration of different types of functionalized multiwalled carbon nanotubes},
author = {Lara Lacerda and Hanene Ali-Boucetta and Maria A Herrero and Giorgia Pastorin and Alberto Bianco and Maurizio Prato and Kostas Kostarelos},
doi = {10.2217/17435889.3.2.149},
issn = {1748-6963},
year = {2008},
date = {2008-04-01},
journal = {Nanomedicine (London, England)},
volume = {3},
number = {2},
pages = {149--161},
abstract = {BACKGROUND: Carbon nanotubes (CNTs) constitute one of the most important types of nanomaterials, increasingly gaining interest as tools for nanomedicine applications, such as sensors, implants or delivery systems. Our groups have reported previously that chemical functionalization of CNTs can lead to their almost complete elimination from the body of animals through the urinary excretion route. The administration of CNTs may, however, impact the physiological function of organs through which CNTs traverse or accumulate. AIM: The present study addresses the short-term impact (first 24 h) of intravenous administration of various types of multiwalled nanotubes (MWNTs) on the physiology of healthy mice. MATERIALS & METHODS: Nonfunctionalized, purified MWNTs (pMWNTs) and different types of water-dispersible, functionalized MWNTs (f-MWNTs) were tail-vein injected. Histological examination of tissues (kidney, liver, spleen and lung) harvested 24 h post-administration indicated that organ accumulation depended on the degree of ammonium (NH(3)(+)) functionalization at the f-MWNT surface. RESULTS: The higher the degree of functionalization of MWNT-NH(3)(+), the less their accumulation in tissues. pMWNTs coated with autologous serum proteins prior to injection accumulated almost entirely in the lung and liver in large dark clusters. Moreover, various indicators of serum and urine analyses also confirmed that MWNT-NH(3)(+) injections did not induce any physiological abnormality in all major organs within the first 24 h post-injection. Interestingly, no abnormalities were observed either for f-MWNTs highly functionalized with carboxylate groups (diethylentriaminepentaacetic-functionalized MWNTs) or by upscaling to the highest doses ever injected so far in vivo (20 mg/kg). CONCLUSION: The high degree of f-MWNT functionalization responsible for adequate individualization of nanotubes and not the nature of the functional groups was the critical factor leading to less tissue accumulation and normal tissue physiology at least within the first 24 h post-administration, even at the highest carbon nanotube doses ever administered in any study today.},
keywords = {Animals, carbon, Female, I2CT, Inbred BALB C, Injections, Intravenous, Mice, Nanotubes, Organ Specificity, Team-Bianco, Tissue Distribution},
pubstate = {published},
tppubtype = {article}
}
Goulev Youlian, Fauny Jean Daniel, Gonzalez-Marti Beatriz, Flagiello Domenico, Silber Joël, Zider Alain
SCALLOPED interacts with YORKIE, the nuclear effector of the hippo tumor-suppressor pathway in Drosophila Article de journal
Dans: Current Biology: CB, vol. 18, no. 6, p. 435–441, 2008, ISSN: 0960-9822.
Résumé | Liens | BibTeX | Étiquettes: Animals, Cell Proliferation, Drosophila, Drosophila Proteins, HeLa Cells, Humans, I2CT, Imagerie, Intracellular Signaling Peptides and Proteins, Morphogenesis, Nuclear Proteins, Protein Kinases, Protein-Serine-Threonine Kinases, Signal Transduction, Trans-Activators, Transcription Factors, Tumor Suppressor Proteins, Wing
@article{goulev_scalloped_2008,
title = {SCALLOPED interacts with YORKIE, the nuclear effector of the hippo tumor-suppressor pathway in Drosophila},
author = {Youlian Goulev and Jean Daniel Fauny and Beatriz Gonzalez-Marti and Domenico Flagiello and Joël Silber and Alain Zider},
url = {http://www.ncbi.nlm.nih.gov/pubmed/18313299},
doi = {10.1016/j.cub.2008.02.034},
issn = {0960-9822},
year = {2008},
date = {2008-01-01},
urldate = {2011-10-24},
journal = {Current Biology: CB},
volume = {18},
number = {6},
pages = {435--441},
abstract = {In Drosophila, SCALLOPED (SD) belongs to a family of evolutionarily conserved proteins characterized by the presence of a TEA/ATTS DNA-binding domain [1, 2]. SD physically interacts with the product of the vestigial (vg) gene, where the dimer functions as a master gene controlling wing formation [3, 4]. The VG-SD dimer activates the transcription of several specific wing genes, including sd and vg themselves [5, 6]. The dimer drives cell-cycle progression by inducing expression of the dE2F1 transcription factor [7], which regulates genes involved in DNA replication and cell-cycle progression. Recently, YORKIE (YKI) was identified as a transcriptional coactivator that is the downstream effector of the Hippo signaling pathway, which controls cell proliferation and apoptosis in Drosophila[8]. We identified SD as a partner for YKI. We show that interaction between YKI and SD increases SD transcriptional activity both ex vivo in Drosophila S2 cells and in vivo in Drosophila wing discs and promotes YKI nuclear localization. We also show that YKI overexpression induces vg and dE2F1 expression and that proliferation induced by YKI or by a dominant-negative form of FAT in wing disc is significantly reduced in a sd hypomorphic mutant context. Contrary to YKI, SD is not required in all imaginal tissues. This indicates that YKI-SD interaction acts in a tissue-specific fashion and that other YKI partners must exist.},
keywords = {Animals, Cell Proliferation, Drosophila, Drosophila Proteins, HeLa Cells, Humans, I2CT, Imagerie, Intracellular Signaling Peptides and Proteins, Morphogenesis, Nuclear Proteins, Protein Kinases, Protein-Serine-Threonine Kinases, Signal Transduction, Trans-Activators, Transcription Factors, Tumor Suppressor Proteins, Wing},
pubstate = {published},
tppubtype = {article}
}
Muller Sylviane, Monneaux Fanny, Schall Nicolas, Rashkov Rasho K, Oparanov Boycho A, Wiesel Philippe, Geiger Jean-Marie, Zimmer Robert
Spliceosomal peptide P140 for immunotherapy of systemic lupus erythematosus: results of an early phase II clinical trial Article de journal
Dans: Arthritis and Rheumatism, vol. 58, no. 12, p. 3873–3883, 2008, ISSN: 0004-3591.
Résumé | Liens | BibTeX | Étiquettes: Adolescent, Adult, Aged, Antibodies, Antinuclear, C-Reactive Protein, DNA, Female, Humans, I2CT, Immunotherapy, Lupus Erythematosus, Male, Middle Aged, Monneaux, Peptide Fragments, Peptides, Severity of Illness Index, Spliceosomes, Systemic, Team-Dumortier, Treatment Outcome, Young Adult
@article{muller_spliceosomal_2008,
title = {Spliceosomal peptide P140 for immunotherapy of systemic lupus erythematosus: results of an early phase II clinical trial},
author = {Sylviane Muller and Fanny Monneaux and Nicolas Schall and Rasho K Rashkov and Boycho A Oparanov and Philippe Wiesel and Jean-Marie Geiger and Robert Zimmer},
doi = {10.1002/art.24027},
issn = {0004-3591},
year = {2008},
date = {2008-01-01},
journal = {Arthritis and Rheumatism},
volume = {58},
number = {12},
pages = {3873--3883},
abstract = {OBJECTIVE: To assess the safety, tolerability, and efficacy of spliceosomal peptide P140 (IPP-201101; sequence 131-151 of the U1-70K protein phosphorylated at Ser140), which is recognized by lupus CD4+ T cells, in the treatment of patients with systemic lupus erythematosus (SLE).
METHODS: An open-label, dose-escalation phase II study was conducted in two centers in Bulgaria. Twenty patients (2 male and 18 female) with moderately active SLE received 3 subcutaneous (SC) administrations of a clinical batch of P140 peptide at 2-week intervals. Clinical evaluation was performed using approved scales. A panel of autoantibodies, including antinuclear antibodies, antibodies to extractable nuclear antigens (U1 RNP, SmD1, Ro/SSA, La/SSB), and antibodies to double-stranded DNA (anti-dsDNA), chromatin, cardiolipin, and peptides of the U1-70K protein, was tested by enzyme-linked immunosorbent assay (ELISA). The plasma levels of C-reactive protein, total Ig, IgG, IgG subclasses, IgM, IgA, and IgE, and of the cytokines interleukin-2 and tumor necrosis factor alpha were measured by ELISA and nephelometry.
RESULTS: IgG anti-dsDNA antibody levels decreased by at least 20% in 7 of 10 patients who received 3 x 200 microg IPP-201101 (group 1), but only in 1 patient in the group receiving 3 x 1,000 microg IPP-201101 (group 2). Physician's global assessment of disease activity scores and scores on the SLE Disease Activity Index were significantly decreased in group 1. The changes occurred progressively in the population of responders, increased in magnitude during the treatment period, and were sustained. No clinical or biologic adverse effects were observed in the individuals, except for some local irritation at the highest concentration.
CONCLUSION: IPP-201101 was found to be safe and well tolerated by subjects. Three SC doses of IPP-201101 at 200 microg significantly improved the clinical and biologic status of lupus patients.},
keywords = {Adolescent, Adult, Aged, Antibodies, Antinuclear, C-Reactive Protein, DNA, Female, Humans, I2CT, Immunotherapy, Lupus Erythematosus, Male, Middle Aged, Monneaux, Peptide Fragments, Peptides, Severity of Illness Index, Spliceosomes, Systemic, Team-Dumortier, Treatment Outcome, Young Adult},
pubstate = {published},
tppubtype = {article}
}
Dieker J, Cisterna B, Monneaux F, Decossas M, van der Vlag J, Biggiogera M, Muller S
Apoptosis-linked changes in the phosphorylation status and subcellular localization of the spliceosomal autoantigen U1-70K Article de journal
Dans: Cell Death and Differentiation, vol. 15, no. 4, p. 793–804, 2008, ISSN: 1350-9047.
Résumé | Liens | BibTeX | Étiquettes: Apoptosis, Autoantigens, Autoimmunity, Caspase 3, Chromatin, HeLa Cells, Humans, I2CT, Jurkat Cells, Lupus Erythematosus, Monneaux, Phosphorylation, Post-Translational, Protein Phosphatase 1, Protein Processing, Protein Transport, Recombinant Proteins, Ribonucleoprotein, RNA Splicing, Serine, Spliceosomes, Systemic, Team-Dumortier, Time Factors, U1 Small Nuclear
@article{dieker_apoptosis-linked_2008,
title = {Apoptosis-linked changes in the phosphorylation status and subcellular localization of the spliceosomal autoantigen U1-70K},
author = {J Dieker and B Cisterna and F Monneaux and M Decossas and J van der Vlag and M Biggiogera and S Muller},
doi = {10.1038/sj.cdd.4402312},
issn = {1350-9047},
year = {2008},
date = {2008-01-01},
journal = {Cell Death and Differentiation},
volume = {15},
number = {4},
pages = {793--804},
abstract = {Apoptosis consists of highly regulated pathways involving post-translational modifications and cleavage of proteins leading to sequential inactivation of the main cellular processes. Here, we focused on the apoptotic processing of one of the essential components of the mRNA splicing machinery, the U1-70K snRNP protein. We found that at an early stage of apoptosis, before the cleavage of the C-terminal part of the protein by caspase-3, the basal phosphorylation of the Ser140 residue located within the RNA recognition motif, increases very significantly. A caspase-dependent, PP1-mediated dephosphorylation of other serine residues takes place in a subset of U1-70K proteins. The U1-70K protein phosphorylated at Ser140 is clustered in heterogeneous ectopic RNP-derived structures, which are finally extruded in apoptotic bodies. The elaborate processing of the spliceosomal U1-70K protein we identified might play an important role in the regulated breakdown of the mRNA splicing machinery during early apoptosis. In addition, these specific changes in the phosphorylation/dephosphorylation balance and the subcellular localization of the U1-70K protein might explain why the region encompassing the Ser140 residue becomes a central autoantigen during the autoimmune disease systemic lupus erythematosus.},
keywords = {Apoptosis, Autoantigens, Autoimmunity, Caspase 3, Chromatin, HeLa Cells, Humans, I2CT, Jurkat Cells, Lupus Erythematosus, Monneaux, Phosphorylation, Post-Translational, Protein Phosphatase 1, Protein Processing, Protein Transport, Recombinant Proteins, Ribonucleoprotein, RNA Splicing, Serine, Spliceosomes, Systemic, Team-Dumortier, Time Factors, U1 Small Nuclear},
pubstate = {published},
tppubtype = {article}
}
Kwan Wing-Hong, Navarro-Sanchez Erika, Dumortier Hélène, Decossas Marion, Vachon Hortense, dos Santos Flavia Barreto, Fridman Hervé W, Rey Félix A, Harris Eva, Despres Philippe, Mueller Christopher G
Dermal-type macrophages expressing CD209/DC-SIGN show inherent resistance to dengue virus growth Article de journal
Dans: PLoS neglected tropical diseases, vol. 2, no. 10, p. e311, 2008, ISSN: 1935-2735.
Résumé | Liens | BibTeX | Étiquettes: C-Type, Cell Adhesion Molecules, Cell Line, Cell Surface, Cells, Cultured, Dengue, Dengue virus, Dumortier, Gene Expression, Humans, I2CT, Lectins, Macrophages, Protein Binding, Receptors, Skin, Team-Dumortier, Team-Mueller, Viral Envelope Proteins
@article{kwan_dermal-type_2008,
title = {Dermal-type macrophages expressing CD209/DC-SIGN show inherent resistance to dengue virus growth},
author = {Wing-Hong Kwan and Erika Navarro-Sanchez and Hélène Dumortier and Marion Decossas and Hortense Vachon and Flavia Barreto dos Santos and Hervé W Fridman and Félix A Rey and Eva Harris and Philippe Despres and Christopher G Mueller},
doi = {10.1371/journal.pntd.0000311},
issn = {1935-2735},
year = {2008},
date = {2008-01-01},
journal = {PLoS neglected tropical diseases},
volume = {2},
number = {10},
pages = {e311},
abstract = {BACKGROUND: An important question in dengue pathogenesis is the identity of immune cells involved in the control of dengue virus infection at the site of the mosquito bite. There is evidence that infection of immature myeloid dendritic cells plays a crucial role in dengue pathogenesis and that the interaction of the viral envelope E glycoprotein with CD209/DC-SIGN is a key element for their productive infection. Dermal macrophages express CD209, yet little is known about their role in dengue virus infection.
METHODS AND FINDINGS: Here, we showed that dermal macrophages bound recombinant envelope E glycoprotein fused to green fluorescent protein. Because dermal macrophages stain for IL-10 in situ, we generated dermal-type macrophages from monocytes in the presence of IL-10 to study their infection by dengue virus. The macrophages were able to internalize the virus, but progeny virus production was undetectable in the infected cells. In addition, no IFN-alpha was produced in response to the virus. The inability of dengue virus to grow in the macrophages was attributable to accumulation of internalized virus particles into poorly-acidified phagosomes.
CONCLUSIONS: Aborting infection by viral sequestration in early phagosomes would present a novel means to curb infection of enveloped virus and may constitute a prime defense system to prevent dengue virus spread shortly after the bite of the infected mosquito.},
keywords = {C-Type, Cell Adhesion Molecules, Cell Line, Cell Surface, Cells, Cultured, Dengue, Dengue virus, Dumortier, Gene Expression, Humans, I2CT, Lectins, Macrophages, Protein Binding, Receptors, Skin, Team-Dumortier, Team-Mueller, Viral Envelope Proteins},
pubstate = {published},
tppubtype = {article}
}
Ali-Boucetta Hanene, Al-Jamal Khuloud T, McCarthy David, Prato Maurizio, Bianco Alberto, Kostarelos Kostas
Multiwalled carbon nanotube-doxorubicin supramolecular complexes for cancer therapeutics Article de journal
Dans: Chemical Communications (Cambridge, England), no. 4, p. 459–461, 2008, ISSN: 1359-7345.
Résumé | Liens | BibTeX | Étiquettes: Antineoplastic Agents, Breast Neoplasms, carbon, Cultured, Doxorubicin, Electron, Humans, I2CT, Microscopy, Nanotubes, Team-Bianco, Transmission, Tumor Cells
@article{ali-boucetta_multiwalled_2008,
title = {Multiwalled carbon nanotube-doxorubicin supramolecular complexes for cancer therapeutics},
author = {Hanene Ali-Boucetta and Khuloud T Al-Jamal and David McCarthy and Maurizio Prato and Alberto Bianco and Kostas Kostarelos},
doi = {10.1039/b712350g},
issn = {1359-7345},
year = {2008},
date = {2008-01-01},
journal = {Chemical Communications (Cambridge, England)},
number = {4},
pages = {459--461},
abstract = {Multiwalled carbon nanotube aqueous dispersions using block copolymers are able to form supramolecular complexes with the aromatic chromophore and anticancer agent doxorubicin via pi-pi stacking and enhance its cytotoxic activity.},
keywords = {Antineoplastic Agents, Breast Neoplasms, carbon, Cultured, Doxorubicin, Electron, Humans, I2CT, Microscopy, Nanotubes, Team-Bianco, Transmission, Tumor Cells},
pubstate = {published},
tppubtype = {article}
}